Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene050355 | MS.gene51879 | 0.809062 | 2.28E-50 | -1.69E-46 |
| MS.gene058037 | MS.gene51879 | 0.803242 | 3.83E-49 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Find 44 sgRNAs with CRISPR-Local
Find 69 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| TGATACTGGTCTTATTAATT+TGG | 0.074684 | 7.4:-21849160 | MS.gene51879:CDS |
| GAAGATGATGATAGTGATTA+TGG | 0.245383 | 7.4:-21849253 | MS.gene51879:intron |
| GATAAACCTCCTGAGCTTCT+TGG | 0.285689 | 7.4:+21849011 | None:intergenic |
| GATACTGGTCTTATTAATTT+GGG | 0.340633 | 7.4:-21849159 | MS.gene51879:CDS |
| TGTTGACTGTGCCTCGTATA+TGG | 0.343991 | 7.4:-21849681 | MS.gene51879:intron |
| GGGGATGGTGACACTGATTC+TGG | 0.344449 | 7.4:-21849232 | MS.gene51879:CDS |
| CGTGATAAGGATTATGATAC+TGG | 0.381257 | 7.4:-21849174 | MS.gene51879:CDS |
| TTCCAAGATGTTCGTTGAAA+AGG | 0.392455 | 7.4:-21849587 | MS.gene51879:CDS |
| GGCTGATGTTGCAGAAGTTA+AGG | 0.398889 | 7.4:-21849566 | MS.gene51879:CDS |
| GAATCCTCATCTTCAATTGA+TGG | 0.428377 | 7.4:-21849114 | MS.gene51879:CDS |
| GAAGCTTATTCATGCCTTTG+AGG | 0.433366 | 7.4:-21849052 | MS.gene51879:CDS |
| GCCCAAGGTGTTTGTTGAAA+AGG | 0.441839 | 7.4:-21849366 | MS.gene51879:CDS |
| CAATGTCGTTGTTGATCCGC+CGG | 0.464868 | 7.4:-21849497 | MS.gene51879:CDS |
| TGAAACACAAGCTCGTGATA+AGG | 0.467417 | 7.4:-21849187 | MS.gene51879:CDS |
| ACTGTCATCATATTTCAGAT+TGG | 0.468193 | 7.4:+21849086 | None:intergenic |
| TGTGGATGTTGGAGAAGGCT+TGG | 0.478175 | 7.4:-21849539 | MS.gene51879:CDS |
| AAGATGATGATAGTGATTAT+GGG | 0.478679 | 7.4:-21849252 | MS.gene51879:CDS |
| GTTAAGGTTGGTGTGGATGT+TGG | 0.484654 | 7.4:-21849550 | MS.gene51879:CDS |
| ATGATGACAGTTCTGTTAAG+AGG | 0.487072 | 7.4:-21849074 | MS.gene51879:CDS |
| GGTGATGACAACGACATTGT+TGG | 0.523107 | 7.4:-21849138 | MS.gene51879:CDS |
| TGCATGATACCAAGAAGCTC+AGG | 0.537367 | 7.4:-21849020 | MS.gene51879:CDS |
| GTGGAAGCACAGTCAGCAGC+CGG | 0.540550 | 7.4:+21849478 | None:intergenic |
| TGGTTCCTTTAGAGTGAAGA+GGG | 0.555963 | 7.4:-21849747 | MS.gene51879:intron |
| ATGGTTCCTTTAGAGTGAAG+AGG | 0.575437 | 7.4:-21849748 | MS.gene51879:intron |
| TAGAGTGAAGAGGGTGTGCA+AGG | 0.583452 | 7.4:-21849738 | MS.gene51879:CDS |
| TGCAGAAGTTAAGGTTGGTG+TGG | 0.586776 | 7.4:-21849557 | MS.gene51879:CDS |
| GATGATAGTGATTATGGGGA+TGG | 0.586801 | 7.4:-21849247 | MS.gene51879:CDS |
| CGGCGGATCAACAACGACAT+TGG | 0.588229 | 7.4:+21849498 | None:intergenic |
| TCGACCATCAATTGAAGATG+AGG | 0.588932 | 7.4:+21849110 | None:intergenic |
| GTTGGTGTGGATGTTGGAGA+AGG | 0.589946 | 7.4:-21849544 | MS.gene51879:CDS |
| TTCAGACCAGACACCTCAAG+AGG | 0.599996 | 7.4:+21849832 | None:intergenic |
| GATGTTGCAGAAGTTAAGGT+TGG | 0.614135 | 7.4:-21849562 | MS.gene51879:CDS |
| GAGGTTTATCAAGATTGATG+AGG | 0.616411 | 7.4:-21848998 | MS.gene51879:CDS |
| GAAGCACAGTCAGCAGCCGG+CGG | 0.633752 | 7.4:+21849481 | None:intergenic |
| ACAATATAAAATTACCTCGG+TGG | 0.641258 | 7.4:+21849459 | None:intergenic |
| GTGTTTGTTGAAAAGGCTGA+TGG | 0.647320 | 7.4:-21849359 | MS.gene51879:CDS |
| AACAACGACATTGGAAACAG+AGG | 0.647503 | 7.4:+21849507 | None:intergenic |
| AGATGATGATAGTGATTATG+GGG | 0.665226 | 7.4:-21849251 | MS.gene51879:CDS |
| ATGATACCAAGAAGCTCAGG+AGG | 0.668377 | 7.4:-21849017 | MS.gene51879:CDS |
| TGAAAAGGCTGATGGTACAG+AGG | 0.669136 | 7.4:-21849351 | MS.gene51879:intron |
| CATTGCATAGGAATTGCCCA+AGG | 0.671668 | 7.4:-21849381 | MS.gene51879:intron |
| ATGCACAACAGAATCCTCAA+AGG | 0.675171 | 7.4:+21849038 | None:intergenic |
| TGCTGACTGTGCTTCCACCG+AGG | 0.697715 | 7.4:-21849473 | MS.gene51879:intron |
| AGATAACCATACCATATACG+AGG | 0.737693 | 7.4:+21849670 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | TATACAATATAAAATTACCT+CGG | + | chr7.4:21849386-21849405 | None:intergenic | 15.0% |
| !! | TCTATAAATTATTGAATGCT+TGG | - | chr7.4:21849189-21849208 | MS.gene51879:CDS | 20.0% |
| !!! | AATTTAAATGCTTTGTTTGA+TGG | - | chr7.4:21849072-21849091 | MS.gene51879:CDS | 20.0% |
| !! | AAGATGATGATAGTGATTAT+GGG | - | chr7.4:21849587-21849606 | MS.gene51879:CDS | 25.0% |
| !!! | GATACTGGTCTTATTAATTT+GGG | - | chr7.4:21849680-21849699 | MS.gene51879:intron | 25.0% |
| !!! | TGATACTGGTCTTATTAATT+TGG | - | chr7.4:21849679-21849698 | MS.gene51879:intron | 25.0% |
| ACAATATAAAATTACCTCGG+TGG | + | chr7.4:21849383-21849402 | None:intergenic | 30.0% | |
| ACTGTCATCATATTTCAGAT+TGG | + | chr7.4:21849756-21849775 | None:intergenic | 30.0% | |
| GTTAATTTCTTCCATTGCAT+AGG | - | chr7.4:21849446-21849465 | MS.gene51879:intron | 30.0% | |
| TCTACATATGATTGATTGCT+TGG | - | chr7.4:21849410-21849429 | MS.gene51879:intron | 30.0% | |
| ! | AGATGATGATAGTGATTATG+GGG | - | chr7.4:21849588-21849607 | MS.gene51879:CDS | 30.0% |
| ! | GAAGATGATGATAGTGATTA+TGG | - | chr7.4:21849586-21849605 | MS.gene51879:CDS | 30.0% |
| AGATAACCATACCATATACG+AGG | + | chr7.4:21849172-21849191 | None:intergenic | 35.0% | |
| ATGATGACAGTTCTGTTAAG+AGG | - | chr7.4:21849765-21849784 | MS.gene51879:intron | 35.0% | |
| CGTGATAAGGATTATGATAC+TGG | - | chr7.4:21849665-21849684 | MS.gene51879:intron | 35.0% | |
| CTCTAACAGTGATCTTAATG+AGG | - | chr7.4:21849552-21849571 | MS.gene51879:CDS | 35.0% | |
| CTTAGTTTCTTCCATTGCAT+AGG | - | chr7.4:21849225-21849244 | MS.gene51879:CDS | 35.0% | |
| GAATCCTCATCTTCAATTGA+TGG | - | chr7.4:21849725-21849744 | MS.gene51879:CDS | 35.0% | |
| GAGGTTTATCAAGATTGATG+AGG | - | chr7.4:21849841-21849860 | MS.gene51879:intron | 35.0% | |
| TTCCAAGATGTTCGTTGAAA+AGG | - | chr7.4:21849252-21849271 | MS.gene51879:CDS | 35.0% | |
| !! | ATTGCTTGGTTTGCTTAATG+TGG | - | chr7.4:21849424-21849443 | MS.gene51879:intron | 35.0% |
| AACAACGACATTGGAAACAG+AGG | + | chr7.4:21849335-21849354 | None:intergenic | 40.0% | |
| ATGCACAACAGAATCCTCAA+AGG | + | chr7.4:21849804-21849823 | None:intergenic | 40.0% | |
| CTGATGGTACAGAGGTTAAA+AGG | - | chr7.4:21849496-21849515 | MS.gene51879:CDS | 40.0% | |
| CTTGGAAGATTCCTATGCAA+TGG | + | chr7.4:21849239-21849258 | None:intergenic | 40.0% | |
| GAAGCTTATTCATGCCTTTG+AGG | - | chr7.4:21849787-21849806 | MS.gene51879:intron | 40.0% | |
| GATGTTGCAGAAGTTAAGGT+TGG | - | chr7.4:21849277-21849296 | MS.gene51879:intron | 40.0% | |
| TCGACCATCAATTGAAGATG+AGG | + | chr7.4:21849732-21849751 | None:intergenic | 40.0% | |
| TGAAACACAAGCTCGTGATA+AGG | - | chr7.4:21849652-21849671 | MS.gene51879:intron | 40.0% | |
| ! | AGCCTTTTCAACAAACACCT+TGG | + | chr7.4:21849478-21849497 | None:intergenic | 40.0% |
| ! | AGCCTTTTCAACGAACATCT+TGG | + | chr7.4:21849257-21849276 | None:intergenic | 40.0% |
| ! | AGGTTAAAAGGTGTGGATGT+TGG | - | chr7.4:21849508-21849527 | MS.gene51879:CDS | 40.0% |
| ! | GATGATAGTGATTATGGGGA+TGG | - | chr7.4:21849592-21849611 | MS.gene51879:CDS | 40.0% |
| ! | GCCTTTTCAACAAACACCTT+GGG | + | chr7.4:21849477-21849496 | None:intergenic | 40.0% |
| ! | GTGTTTGTTGAAAAGGCTGA+TGG | - | chr7.4:21849480-21849499 | MS.gene51879:CDS | 40.0% |
| !! | ATGGTTCCTTTAGAGTGAAG+AGG | - | chr7.4:21849091-21849110 | MS.gene51879:CDS | 40.0% |
| !! | TGGTTCCTTTAGAGTGAAGA+GGG | - | chr7.4:21849092-21849111 | MS.gene51879:CDS | 40.0% |
| !!! | TGATGGATTTTGTCCTCTTG+AGG | - | chr7.4:21848994-21849013 | MS.gene51879:CDS | 40.0% |
| ACTGTGCCTCGTATATGGTA+TGG | - | chr7.4:21849163-21849182 | MS.gene51879:CDS | 45.0% | |
| ATGATACCAAGAAGCTCAGG+AGG | - | chr7.4:21849822-21849841 | MS.gene51879:intron | 45.0% | |
| CATTGCATAGGAATTGCCCA+AGG | - | chr7.4:21849458-21849477 | MS.gene51879:intron | 45.0% | |
| CTTGGGCAATTCCTATGCAA+TGG | + | chr7.4:21849460-21849479 | None:intergenic | 45.0% | |
| GATAAACCTCCTGAGCTTCT+TGG | + | chr7.4:21849831-21849850 | None:intergenic | 45.0% | |
| GGCTGATGTTGCAGAAGTTA+AGG | - | chr7.4:21849273-21849292 | MS.gene51879:intron | 45.0% | |
| GGTACAGAGGTTAAAAGGTG+TGG | - | chr7.4:21849501-21849520 | MS.gene51879:CDS | 45.0% | |
| GGTGATGACAACGACATTGT+TGG | - | chr7.4:21849701-21849720 | MS.gene51879:CDS | 45.0% | |
| TGAAAAGGCTGATGGTACAG+AGG | - | chr7.4:21849488-21849507 | MS.gene51879:CDS | 45.0% | |
| TGCATGATACCAAGAAGCTC+AGG | - | chr7.4:21849819-21849838 | MS.gene51879:intron | 45.0% | |
| ! | AAAGGTGTGGATGTTGGAGA+AGG | - | chr7.4:21849514-21849533 | MS.gene51879:CDS | 45.0% |
| ! | GCCCAAGGTGTTTGTTGAAA+AGG | - | chr7.4:21849473-21849492 | MS.gene51879:intron | 45.0% |
| ! | TGTTGACTGTGCCTCGTATA+TGG | - | chr7.4:21849158-21849177 | MS.gene51879:CDS | 45.0% |
| !! | GTTAAGGTTGGTGTGGATGT+TGG | - | chr7.4:21849289-21849308 | MS.gene51879:intron | 45.0% |
| !! | TGCAGAAGTTAAGGTTGGTG+TGG | - | chr7.4:21849282-21849301 | MS.gene51879:intron | 45.0% |
| !! | TTTTGTCCTCTTGAGGTGTC+TGG | - | chr7.4:21849001-21849020 | MS.gene51879:CDS | 45.0% |
| CAATGTCGTTGTTGATCCGC+CGG | - | chr7.4:21849342-21849361 | MS.gene51879:intron | 50.0% | |
| GCACACCCTCTTCACTCTAA+AGG | + | chr7.4:21849100-21849119 | None:intergenic | 50.0% | |
| GTTGGAGAAGGCTTGGATGT+TGG | - | chr7.4:21849307-21849326 | MS.gene51879:intron | 50.0% | |
| GTTGGTGTGGATGTTGGAGA+AGG | - | chr7.4:21849295-21849314 | MS.gene51879:intron | 50.0% | |
| TGTGGATGTTGGAGAAGGCT+TGG | - | chr7.4:21849300-21849319 | MS.gene51879:intron | 50.0% | |
| TTCAGACCAGACACCTCAAG+AGG | + | chr7.4:21849010-21849029 | None:intergenic | 50.0% | |
| ! | CTTGAGGTGTCTGGTCTGAA+GGG | - | chr7.4:21849010-21849029 | MS.gene51879:CDS | 50.0% |
| ! | TAGAGTGAAGAGGGTGTGCA+AGG | - | chr7.4:21849101-21849120 | MS.gene51879:CDS | 50.0% |
| ! | TCTTGAGGTGTCTGGTCTGA+AGG | - | chr7.4:21849009-21849028 | MS.gene51879:CDS | 50.0% |
| !! | TGTCTGGTCTGAAGGGTATC+AGG | - | chr7.4:21849017-21849036 | MS.gene51879:CDS | 50.0% |
| CGGCGGATCAACAACGACAT+TGG | + | chr7.4:21849344-21849363 | None:intergenic | 55.0% | |
| !! | GGGGATGGTGACACTGATTC+TGG | - | chr7.4:21849607-21849626 | MS.gene51879:intron | 55.0% |
| ! | GTGGAAGCACAGTCAGCAGC+CGG | + | chr7.4:21849364-21849383 | None:intergenic | 60.0% |
| ! | TGCTGACTGTGCTTCCACCG+AGG | - | chr7.4:21849366-21849385 | MS.gene51879:CDS | 60.0% |
| GAAGCACAGTCAGCAGCCGG+CGG | + | chr7.4:21849361-21849380 | None:intergenic | 65.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr7.4 | gene | 21848993 | 21849868 | 21848993 | ID=MS.gene51879 |
| chr7.4 | mRNA | 21848993 | 21849868 | 21848993 | ID=MS.gene51879.t1;Parent=MS.gene51879 |
| chr7.4 | exon | 21849846 | 21849868 | 21849846 | ID=MS.gene51879.t1.exon1;Parent=MS.gene51879.t1 |
| chr7.4 | CDS | 21849846 | 21849868 | 21849846 | ID=cds.MS.gene51879.t1;Parent=MS.gene51879.t1 |
| chr7.4 | exon | 21849682 | 21849757 | 21849682 | ID=MS.gene51879.t1.exon2;Parent=MS.gene51879.t1 |
| chr7.4 | CDS | 21849682 | 21849757 | 21849682 | ID=cds.MS.gene51879.t1;Parent=MS.gene51879.t1 |
| chr7.4 | exon | 21849474 | 21849614 | 21849474 | ID=MS.gene51879.t1.exon3;Parent=MS.gene51879.t1 |
| chr7.4 | CDS | 21849474 | 21849614 | 21849474 | ID=cds.MS.gene51879.t1;Parent=MS.gene51879.t1 |
| chr7.4 | exon | 21849352 | 21849393 | 21849352 | ID=MS.gene51879.t1.exon4;Parent=MS.gene51879.t1 |
| chr7.4 | CDS | 21849352 | 21849393 | 21849352 | ID=cds.MS.gene51879.t1;Parent=MS.gene51879.t1 |
| chr7.4 | exon | 21848993 | 21849271 | 21848993 | ID=MS.gene51879.t1.exon5;Parent=MS.gene51879.t1 |
| chr7.4 | CDS | 21848993 | 21849271 | 21848993 | ID=cds.MS.gene51879.t1;Parent=MS.gene51879.t1 |
| Gene Sequence |
| Protein sequence |
