Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene51991.t1 | XP_003598365.1 | 98.2 | 325 | 4 | 1 | 1 | 323 | 1 | 325 | 7.00E-177 | 629.8 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene51991.t1 | G7IVH8 | 98.2 | 325 | 4 | 1 | 1 | 323 | 1 | 325 | 5.1e-177 | 629.8 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene051719 | MS.gene51991 | 0.818333 | 2.07E-52 | -1.69E-46 |
| MS.gene051720 | MS.gene51991 | 0.823244 | 1.54E-53 | -1.69E-46 |
| MS.gene053122 | MS.gene51991 | 0.809728 | 1.64E-50 | -1.69E-46 |
| MS.gene054584 | MS.gene51991 | 0.80232 | 5.93E-49 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene51991.t1 | MTR_3g010800 | 98.154 | 325 | 4 | 1 | 1 | 323 | 1 | 325 | 0.0 | 661 |
| MS.gene51991.t1 | MTR_3g062840 | 34.743 | 331 | 169 | 14 | 7 | 318 | 8 | 310 | 1.45e-38 | 139 |
| MS.gene51991.t1 | MTR_5g075750 | 33.125 | 320 | 190 | 12 | 1 | 318 | 4 | 301 | 1.74e-37 | 135 |
| MS.gene51991.t1 | MTR_8g461170 | 29.450 | 309 | 187 | 10 | 17 | 318 | 10 | 294 | 2.65e-29 | 114 |
| MS.gene51991.t1 | MTR_7g074120 | 28.660 | 321 | 199 | 9 | 7 | 317 | 13 | 313 | 4.46e-27 | 108 |
| MS.gene51991.t1 | MTR_8g028165 | 27.465 | 284 | 177 | 10 | 37 | 314 | 34 | 294 | 1.68e-21 | 92.8 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene51991.t1 | AT4G22900 | 47.059 | 340 | 149 | 9 | 1 | 317 | 1 | 332 | 1.14e-97 | 292 |
| MS.gene51991.t1 | AT4G11950 | 42.814 | 334 | 156 | 9 | 8 | 317 | 4 | 326 | 2.49e-85 | 260 |
| MS.gene51991.t1 | AT1G62981 | 40.588 | 340 | 170 | 13 | 3 | 318 | 10 | 341 | 2.28e-64 | 207 |
| MS.gene51991.t1 | AT1G62981 | 40.588 | 340 | 170 | 13 | 3 | 318 | 10 | 341 | 2.28e-64 | 207 |
| MS.gene51991.t1 | AT3G08600 | 32.550 | 298 | 161 | 13 | 33 | 313 | 37 | 311 | 1.41e-22 | 96.3 |
| MS.gene51991.t1 | AT4G23720 | 27.397 | 292 | 185 | 13 | 26 | 313 | 38 | 306 | 3.12e-18 | 84.0 |
| MS.gene51991.t1 | AT4G01140 | 27.645 | 293 | 186 | 12 | 27 | 316 | 28 | 297 | 1.76e-16 | 79.0 |
| MS.gene51991.t1 | AT1G62981 | 35.676 | 185 | 102 | 8 | 146 | 318 | 29 | 208 | 3.05e-16 | 76.6 |
Find 41 sgRNAs with CRISPR-Local
Find 50 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| GCTAAAACACATGGACATTT+TGG | 0.175170 | 3.3:-6093656 | MS.gene51991:CDS |
| TTCCATTGACTTAGTCTTAT+AGG | 0.189682 | 3.3:+6093587 | None:intergenic |
| AGTACTATTGGTGCTGCTTT+AGG | 0.264423 | 3.3:-6093551 | MS.gene51991:CDS |
| AAGCTACCTTCAAATTTATC+AGG | 0.315618 | 3.3:-6094124 | MS.gene51991:CDS |
| CCTTCAAAACTTGCACATAA+TGG | 0.317349 | 3.3:+6093734 | None:intergenic |
| CAACTTGTTTCTCCAATTGT+TGG | 0.336972 | 3.3:-6093905 | MS.gene51991:CDS |
| TAAGCTAAAATTCCAACAAT+TGG | 0.343703 | 3.3:+6093893 | None:intergenic |
| TTGTTGTCATTGGAATTTCA+TGG | 0.351140 | 3.3:-6094256 | MS.gene51991:CDS |
| TTCAACAACCAAGAAGAAAA+AGG | 0.352105 | 3.3:-6093770 | MS.gene51991:CDS |
| GTTGGTGTTGTTCTAGTTCC+AGG | 0.353242 | 3.3:+6093386 | None:intergenic |
| TTATGAAGAAGAAGCACTTC+AGG | 0.366104 | 3.3:-6093444 | MS.gene51991:CDS |
| GCATAGAGTATAATAATAAT+TGG | 0.373049 | 3.3:-6093970 | MS.gene51991:CDS |
| AAGGTATGGTGCAAAATTAA+AGG | 0.383254 | 3.3:-6094059 | MS.gene51991:CDS |
| AAGGAGTTTCATCTTGGTAT+TGG | 0.399934 | 3.3:-6094040 | MS.gene51991:CDS |
| ATTGCAGTTGGGAGTACTAT+TGG | 0.400361 | 3.3:-6093563 | MS.gene51991:CDS |
| TTGATGCCTGATAAATTTGA+AGG | 0.417038 | 3.3:+6094118 | None:intergenic |
| ACACAAACATTTGTTGCTAA+AGG | 0.423104 | 3.3:+6093680 | None:intergenic |
| GCACTTCAGGTTTCAATGGT+TGG | 0.434477 | 3.3:-6093431 | MS.gene51991:CDS |
| AGAAGCACTTCAGGTTTCAA+TGG | 0.442190 | 3.3:-6093435 | MS.gene51991:CDS |
| GCTTCAATTCCAAGTTGAAA+AGG | 0.444070 | 3.3:+6093836 | None:intergenic |
| AAATTAAAGGAGTTTCATCT+TGG | 0.452708 | 3.3:-6094046 | MS.gene51991:CDS |
| AACATTACTCTTTCAATACA+TGG | 0.463603 | 3.3:+6094004 | None:intergenic |
| TTTCAACTTGGAATTGAAGC+TGG | 0.480245 | 3.3:-6093833 | MS.gene51991:CDS |
| TTAGACAAAGATTTCAAGAC+AGG | 0.484697 | 3.3:-6094163 | MS.gene51991:CDS |
| GTTCTAGTTCCAGGTGCAGT+AGG | 0.488529 | 3.3:+6093395 | None:intergenic |
| TGAATGTGTAATCTCTTATG+AGG | 0.490113 | 3.3:+6094191 | None:intergenic |
| GTCAATGGAAAATTGCAGTT+GGG | 0.491640 | 3.3:-6093574 | MS.gene51991:CDS |
| AGCCTATAAGACTAAGTCAA+TGG | 0.492496 | 3.3:-6093589 | MS.gene51991:CDS |
| GGTTAAGAAAAGGTCAAGAA+TGG | 0.493692 | 3.3:-6093486 | MS.gene51991:CDS |
| GAAGGTAGCTTCACTGATTG+TGG | 0.501570 | 3.3:+6094136 | None:intergenic |
| TGTGGAAGTTTAACAAGGTA+TGG | 0.509899 | 3.3:-6094073 | MS.gene51991:CDS |
| AGTCAATGGAAAATTGCAGT+TGG | 0.516447 | 3.3:-6093575 | MS.gene51991:CDS |
| AAGGTCAAGAATGGTTCAGA+TGG | 0.524303 | 3.3:-6093477 | MS.gene51991:CDS |
| TGGTTGTTAGGGTTAAGAAA+AGG | 0.555158 | 3.3:-6093496 | MS.gene51991:CDS |
| GTGAGAGCTCCTACTGCACC+TGG | 0.585262 | 3.3:-6093404 | MS.gene51991:CDS |
| GTTTGTGTTGCTAAAACACA+TGG | 0.605683 | 3.3:-6093665 | MS.gene51991:CDS |
| TTAGATGTGGAAGTTTAACA+AGG | 0.623871 | 3.3:-6094078 | MS.gene51991:CDS |
| GATACTGTGAGATTTAGATG+TGG | 0.646729 | 3.3:-6094091 | MS.gene51991:CDS |
| TAGTCATGTTGCTCAAGTGT+TGG | 0.655160 | 3.3:+6093368 | None:intergenic |
| ATGTGTAATCTCTTATGAGG+AGG | 0.665772 | 3.3:+6094194 | None:intergenic |
| TGCAGTAAACCTTATCAACG+AGG | 0.756012 | 3.3:+6093332 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | GCATAGAGTATAATAATAAT+TGG | - | chr3.3:6093671-6093690 | MS.gene51991:CDS | 20.0% |
| ! | AAATTAAAGGAGTTTCATCT+TGG | - | chr3.3:6093595-6093614 | MS.gene51991:CDS | 25.0% |
| ! | AACATTACTCTTTCAATACA+TGG | + | chr3.3:6093640-6093659 | None:intergenic | 25.0% |
| ! | TAAGCTAAAATTCCAACAAT+TGG | + | chr3.3:6093751-6093770 | None:intergenic | 25.0% |
| AAGCTACCTTCAAATTTATC+AGG | - | chr3.3:6093517-6093536 | MS.gene51991:CDS | 30.0% | |
| AAGGTATGGTGCAAAATTAA+AGG | - | chr3.3:6093582-6093601 | MS.gene51991:CDS | 30.0% | |
| TGAATGTGTAATCTCTTATG+AGG | + | chr3.3:6093453-6093472 | None:intergenic | 30.0% | |
| TTAGACAAAGATTTCAAGAC+AGG | - | chr3.3:6093478-6093497 | MS.gene51991:CDS | 30.0% | |
| TTAGATGTGGAAGTTTAACA+AGG | - | chr3.3:6093563-6093582 | MS.gene51991:CDS | 30.0% | |
| TTCAACAACCAAGAAGAAAA+AGG | - | chr3.3:6093871-6093890 | MS.gene51991:CDS | 30.0% | |
| TTGATGCCTGATAAATTTGA+AGG | + | chr3.3:6093526-6093545 | None:intergenic | 30.0% | |
| TTGTTGTCATTGGAATTTCA+TGG | - | chr3.3:6093385-6093404 | MS.gene51991:CDS | 30.0% | |
| ! | ACACAAACATTTGTTGCTAA+AGG | + | chr3.3:6093964-6093983 | None:intergenic | 30.0% |
| ! | AGTAGTAATCCTTTTCAACT+TGG | - | chr3.3:6093796-6093815 | MS.gene51991:CDS | 30.0% |
| ! | CTTTGATACCTTTTTCTTCT+TGG | + | chr3.3:6093882-6093901 | None:intergenic | 30.0% |
| ! | GACATTTTCATTGTTGTCAT+TGG | - | chr3.3:6093375-6093394 | MS.gene51991:CDS | 30.0% |
| ! | TTTTGTAGCAATGGTTGTTA+GGG | - | chr3.3:6094134-6094153 | MS.gene51991:CDS | 30.0% |
| ! | TTTTTGTAGCAATGGTTGTT+AGG | - | chr3.3:6094133-6094152 | MS.gene51991:CDS | 30.0% |
| !! | TTCCATTGACTTAGTCTTAT+AGG | + | chr3.3:6094057-6094076 | None:intergenic | 30.0% |
| !!! | AGGTTTACTTTTTGTAGCAA+TGG | - | chr3.3:6094125-6094144 | MS.gene51991:CDS | 30.0% |
| AAGGAGTTTCATCTTGGTAT+TGG | - | chr3.3:6093601-6093620 | MS.gene51991:CDS | 35.0% | |
| AGCCTATAAGACTAAGTCAA+TGG | - | chr3.3:6094052-6094071 | MS.gene51991:CDS | 35.0% | |
| AGTCAATGGAAAATTGCAGT+TGG | - | chr3.3:6094066-6094085 | MS.gene51991:CDS | 35.0% | |
| ATGTGTAATCTCTTATGAGG+AGG | + | chr3.3:6093450-6093469 | None:intergenic | 35.0% | |
| CCTTCAAAACTTGCACATAA+TGG | + | chr3.3:6093910-6093929 | None:intergenic | 35.0% | |
| GATACTGTGAGATTTAGATG+TGG | - | chr3.3:6093550-6093569 | MS.gene51991:CDS | 35.0% | |
| GGTTAAGAAAAGGTCAAGAA+TGG | - | chr3.3:6094155-6094174 | MS.gene51991:CDS | 35.0% | |
| GTCAATGGAAAATTGCAGTT+GGG | - | chr3.3:6094067-6094086 | MS.gene51991:CDS | 35.0% | |
| TGGTTGTTAGGGTTAAGAAA+AGG | - | chr3.3:6094145-6094164 | MS.gene51991:CDS | 35.0% | |
| TGTGGAAGTTTAACAAGGTA+TGG | - | chr3.3:6093568-6093587 | MS.gene51991:CDS | 35.0% | |
| TTTCAACTTGGAATTGAAGC+TGG | - | chr3.3:6093808-6093827 | MS.gene51991:CDS | 35.0% | |
| ! | CAACTTGTTTCTCCAATTGT+TGG | - | chr3.3:6093736-6093755 | MS.gene51991:CDS | 35.0% |
| ! | GCTAAAACACATGGACATTT+TGG | - | chr3.3:6093985-6094004 | MS.gene51991:CDS | 35.0% |
| ! | GCTTCAATTCCAAGTTGAAA+AGG | + | chr3.3:6093808-6093827 | None:intergenic | 35.0% |
| ! | GTTTGTGTTGCTAAAACACA+TGG | - | chr3.3:6093976-6093995 | MS.gene51991:CDS | 35.0% |
| !! | CCATTATGTGCAAGTTTTGA+AGG | - | chr3.3:6093907-6093926 | MS.gene51991:CDS | 35.0% |
| !! | GCTTTAGGTGCTTTTCTTTT+AGG | - | chr3.3:6094105-6094124 | MS.gene51991:CDS | 35.0% |
| !! | TTATGAAGAAGAAGCACTTC+AGG | - | chr3.3:6094197-6094216 | MS.gene51991:CDS | 35.0% |
| !!! | AACACATGGACATTTTGGTT+TGG | - | chr3.3:6093990-6094009 | MS.gene51991:CDS | 35.0% |
| !!! | TGTGCAAGTTTTGAAGGAAA+TGG | - | chr3.3:6093913-6093932 | MS.gene51991:CDS | 35.0% |
| AAGGTCAAGAATGGTTCAGA+TGG | - | chr3.3:6094164-6094183 | MS.gene51991:CDS | 40.0% | |
| TAGTCATGTTGCTCAAGTGT+TGG | + | chr3.3:6094276-6094295 | None:intergenic | 40.0% | |
| !! | AGAAGCACTTCAGGTTTCAA+TGG | - | chr3.3:6094206-6094225 | MS.gene51991:CDS | 40.0% |
| !! | AGTACTATTGGTGCTGCTTT+AGG | - | chr3.3:6094090-6094109 | MS.gene51991:CDS | 40.0% |
| !! | ATTGCAGTTGGGAGTACTAT+TGG | - | chr3.3:6094078-6094097 | MS.gene51991:CDS | 40.0% |
| ! | GAAGGTAGCTTCACTGATTG+TGG | + | chr3.3:6093508-6093527 | None:intergenic | 45.0% |
| ! | GCACTTCAGGTTTCAATGGT+TGG | - | chr3.3:6094210-6094229 | MS.gene51991:CDS | 45.0% |
| !! | GTTGGTGTTGTTCTAGTTCC+AGG | + | chr3.3:6094258-6094277 | None:intergenic | 45.0% |
| !! | GTTCTAGTTCCAGGTGCAGT+AGG | + | chr3.3:6094249-6094268 | None:intergenic | 50.0% |
| GTGAGAGCTCCTACTGCACC+TGG | - | chr3.3:6094237-6094256 | MS.gene51991:CDS | 60.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr3.3 | gene | 6093346 | 6094317 | 6093346 | ID=MS.gene51991 |
| chr3.3 | mRNA | 6093346 | 6094317 | 6093346 | ID=MS.gene51991.t1;Parent=MS.gene51991 |
| chr3.3 | exon | 6093346 | 6094317 | 6093346 | ID=MS.gene51991.t1.exon1;Parent=MS.gene51991.t1 |
| chr3.3 | CDS | 6093346 | 6094317 | 6093346 | ID=cds.MS.gene51991.t1;Parent=MS.gene51991.t1 |
| Gene Sequence |
| Protein sequence |
