Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene52240.t1 | AES80150.1 | 94 | 50 | 3 | 0 | 1 | 50 | 1 | 50 | 5.70E-17 | 96.7 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene52240.t1 | Q41066 | 47.8 | 67 | 18 | 1 | 3 | 52 | 4 | 70 | 3.2e-08 | 58.9 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene52240.t1 | G7L3N0 | 94.0 | 50 | 3 | 0 | 1 | 50 | 1 | 50 | 4.1e-17 | 96.7 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene055818 | MS.gene52240 | 0.809289 | 2.04E-50 | -1.69E-46 |
| MS.gene056366 | MS.gene52240 | 0.81119 | 7.92E-51 | -1.69E-46 |
| MS.gene056595 | MS.gene52240 | 0.817913 | 2.58E-52 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene52240.t1 | MTR_7g077180 | 96.512 | 86 | 3 | 0 | 1 | 86 | 1 | 86 | 2.25e-55 | 166 |
| MS.gene52240.t1 | MTR_7g077340 | 61.818 | 110 | 18 | 2 | 1 | 86 | 3 | 112 | 2.94e-38 | 124 |
| MS.gene52240.t1 | MTR_7g077215 | 62.887 | 97 | 15 | 1 | 11 | 86 | 14 | 110 | 4.02e-35 | 115 |
| MS.gene52240.t1 | MTR_7g077210 | 51.724 | 116 | 23 | 5 | 1 | 86 | 4 | 116 | 6.63e-25 | 90.1 |
| MS.gene52240.t1 | MTR_7g077300 | 48.544 | 103 | 35 | 3 | 1 | 86 | 1 | 102 | 1.73e-23 | 86.3 |
| MS.gene52240.t1 | MTR_7g077260 | 45.133 | 113 | 35 | 3 | 1 | 86 | 1 | 113 | 1.97e-22 | 83.6 |
| MS.gene52240.t1 | MTR_7g077310 | 47.115 | 104 | 28 | 2 | 9 | 86 | 1 | 103 | 4.49e-22 | 82.4 |
| MS.gene52240.t1 | MTR_7g077160 | 54.930 | 71 | 32 | 0 | 16 | 86 | 30 | 100 | 8.08e-21 | 79.3 |
| MS.gene52240.t1 | MTR_7g077220 | 46.875 | 96 | 16 | 2 | 8 | 80 | 1 | 84 | 1.39e-17 | 72.0 |
| MS.gene52240.t1 | MTR_7g077190 | 56.897 | 58 | 25 | 0 | 29 | 86 | 22 | 79 | 2.84e-17 | 69.7 |
| MS.gene52240.t1 | MTR_7g077313 | 38.384 | 99 | 39 | 3 | 3 | 81 | 4 | 100 | 7.53e-13 | 58.9 |
| MS.gene52240.t1 | MTR_7g077350 | 41.228 | 114 | 36 | 5 | 1 | 85 | 20 | 131 | 6.13e-11 | 54.7 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Find 13 sgRNAs with CRISPR-Local
Find 18 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| CAACATAGTTAATTACATTT+TGG | 0.190001 | 7.4:+28731529 | None:intergenic |
| AATGATGAAGTTAGCTTTGT+TGG | 0.284953 | 7.4:-28731755 | MS.gene52240:CDS |
| CAATGCCGTTGTCGTGATAT+TGG | 0.339584 | 7.4:-28731643 | MS.gene52240:CDS |
| CTTGGGCTTCACTTCAACCA+TGG | 0.414441 | 7.4:-28731725 | MS.gene52240:CDS |
| CACGACAACGGCATTGAGGA+GGG | 0.589377 | 7.4:+28731650 | None:intergenic |
| TCACGACAACGGCATTGAGG+AGG | 0.620003 | 7.4:+28731649 | None:intergenic |
| GCAGGAATCACAGCATGCAG+TGG | 0.649347 | 7.4:+28731690 | None:intergenic |
| TGGTTGAAGTGAAGCCCAAG+AGG | 0.657022 | 7.4:+28731728 | None:intergenic |
| ATATCACGACAACGGCATTG+AGG | 0.657096 | 7.4:+28731646 | None:intergenic |
| TCAGCGCAGTGACACTGTGG+AGG | 0.657650 | 7.4:+28731571 | None:intergenic |
| TTTCTCCAATATCACGACAA+CGG | 0.709966 | 7.4:+28731638 | None:intergenic |
| ATATCAGCGCAGTGACACTG+TGG | 0.729166 | 7.4:+28731568 | None:intergenic |
| AGTGGTTGTAGCATCAACCA+TGG | 0.768073 | 7.4:+28731708 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | AATGATGAAGTTAGCTTTGT+TGG | - | chr7.4:28731557-28731576 | MS.gene52240:CDS | 30.0% |
| TTTCTCCAATATCACGACAA+CGG | + | chr7.4:28731677-28731696 | None:intergenic | 35.0% | |
| ! | GAGGAATAGATTTTGTGCAA+AGG | + | chr7.4:28731725-28731744 | None:intergenic | 35.0% |
| !! | GAGGGATTGATTTTGTGCAA+GGG | + | chr7.4:28731647-28731666 | None:intergenic | 40.0% |
| !!! | AGCTTTGTTGGTTTTCCTCT+TGG | - | chr7.4:28731569-28731588 | MS.gene52240:CDS | 40.0% |
| !!! | GCTTTGTTGGTTTTCCTCTT+GGG | - | chr7.4:28731570-28731589 | MS.gene52240:CDS | 40.0% |
| AGTGGTTGTAGCATCAACCA+TGG | + | chr7.4:28731607-28731626 | None:intergenic | 45.0% | |
| ATATCACGACAACGGCATTG+AGG | + | chr7.4:28731669-28731688 | None:intergenic | 45.0% | |
| CAATGCCGTTGTCGTGATAT+TGG | - | chr7.4:28731669-28731688 | MS.gene52240:CDS | 45.0% | |
| !! | GATTGATTTTGTGCAAGGGC+AGG | + | chr7.4:28731643-28731662 | None:intergenic | 45.0% |
| !! | GGAGGGATTGATTTTGTGCA+AGG | + | chr7.4:28731648-28731667 | None:intergenic | 45.0% |
| ATATCAGCGCAGTGACACTG+TGG | + | chr7.4:28731747-28731766 | None:intergenic | 50.0% | |
| CTTGGGCTTCACTTCAACCA+TGG | - | chr7.4:28731587-28731606 | MS.gene52240:CDS | 50.0% | |
| TGGTTGAAGTGAAGCCCAAG+AGG | + | chr7.4:28731587-28731606 | None:intergenic | 50.0% | |
| GCAGGAATCACAGCATGCAG+TGG | + | chr7.4:28731625-28731644 | None:intergenic | 55.0% | |
| ! | CACGACAACGGCATTGAGGA+GGG | + | chr7.4:28731665-28731684 | None:intergenic | 55.0% |
| ! | TCACGACAACGGCATTGAGG+AGG | + | chr7.4:28731666-28731685 | None:intergenic | 55.0% |
| TCAGCGCAGTGACACTGTGG+AGG | + | chr7.4:28731744-28731763 | None:intergenic | 60.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr7.4 | gene | 28731537 | 28731797 | 28731537 | ID=MS.gene52240 |
| chr7.4 | mRNA | 28731537 | 28731797 | 28731537 | ID=MS.gene52240.t1;Parent=MS.gene52240 |
| chr7.4 | exon | 28731537 | 28731797 | 28731537 | ID=MS.gene52240.t1.exon1;Parent=MS.gene52240.t1 |
| chr7.4 | CDS | 28731537 | 28731797 | 28731537 | ID=cds.MS.gene52240.t1;Parent=MS.gene52240.t1 |
| Gene Sequence |
| Protein sequence |
