Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene52241.t1 | AES80153.2 | 92.1 | 76 | 5 | 1 | 2 | 76 | 7 | 82 | 1.10E-27 | 132.1 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene52241.t1 | Q8W4Y8 | 63.2 | 76 | 18 | 4 | 1 | 76 | 4 | 69 | 1.3e-13 | 76.6 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene52241.t1 | G7L4K5 | 92.1 | 76 | 5 | 1 | 2 | 76 | 7 | 82 | 7.8e-28 | 132.1 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene051719 | MS.gene52241 | 0.830416 | 2.97E-55 | -1.69E-46 |
| MS.gene051720 | MS.gene52241 | 0.838621 | 2.58E-57 | -1.69E-46 |
| MS.gene053144 | MS.gene52241 | 0.800806 | 1.21E-48 | -1.69E-46 |
| MS.gene053678 | MS.gene52241 | 0.813822 | 2.11E-51 | -1.69E-46 |
| MS.gene059015 | MS.gene52241 | 0.801007 | 1.10E-48 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene52241.t1 | MTR_7g077210 | 92.105 | 76 | 5 | 1 | 2 | 76 | 7 | 82 | 1.68e-43 | 136 |
| MS.gene52241.t1 | MTR_7g077340 | 69.231 | 78 | 17 | 3 | 1 | 76 | 5 | 77 | 1.76e-30 | 103 |
| MS.gene52241.t1 | MTR_7g077220 | 58.140 | 86 | 15 | 3 | 7 | 76 | 1 | 81 | 3.83e-24 | 88.2 |
| MS.gene52241.t1 | MTR_7g077260 | 63.636 | 77 | 23 | 4 | 1 | 76 | 4 | 76 | 2.17e-23 | 85.9 |
| MS.gene52241.t1 | MTR_7g077215 | 62.319 | 69 | 17 | 3 | 10 | 76 | 14 | 75 | 8.22e-20 | 76.6 |
| MS.gene52241.t1 | MTR_7g077310 | 49.296 | 71 | 31 | 4 | 8 | 76 | 1 | 68 | 1.93e-14 | 62.8 |
| MS.gene52241.t1 | MTR_7g077313 | 48.052 | 77 | 31 | 4 | 1 | 76 | 4 | 72 | 5.73e-11 | 53.9 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Find 11 sgRNAs with CRISPR-Local
Find 13 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| ACATGCTGTGGCTGTTGATT+TGG | 0.351329 | 7.4:-28715819 | None:intergenic |
| CAGCACAACGACACTGAGTA+AGG | 0.448584 | 7.4:-28715874 | None:intergenic |
| ATCACTCAAGTGATCTCAAA+TGG | 0.476956 | 7.4:+28715773 | MS.gene52241:CDS |
| TGAGATCACTTGAGTGATGA+AGG | 0.520877 | 7.4:-28715768 | None:intergenic |
| CAGTTGAAGTGAAACCCAAA+AGG | 0.552558 | 7.4:-28715718 | None:intergenic |
| TGGTGTTACTGAGCAAAGGC+AGG | 0.567676 | 7.4:-28715849 | None:intergenic |
| GGAGCTGATCATGAACAAGA+AGG | 0.596023 | 7.4:+28715664 | None:intergenic |
| GGGTTGGTGTTACTGAGCAA+AGG | 0.617598 | 7.4:-28715853 | None:intergenic |
| CAACGACACTGAGTAAGGGT+TGG | 0.634241 | 7.4:-28715869 | None:intergenic |
| GCAGGTATTACAACATGCTG+TGG | 0.640103 | 7.4:-28715831 | None:intergenic |
| AGCACAACGACACTGAGTAA+GGG | 0.641174 | 7.4:-28715873 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| ATCACTCAAGTGATCTCAAA+TGG | + | chr7.4:28715773-28715792 | MS.gene52241:CDS | 35.0% | |
| !!! | AGCTTTTCTGGTTTTCCTTT+TGG | + | chr7.4:28715703-28715722 | MS.gene52241:CDS | 35.0% |
| !!! | GATGATGAAGTTAGCTTTTC+TGG | + | chr7.4:28715691-28715710 | MS.gene52241:CDS | 35.0% |
| !!! | GCTTTTCTGGTTTTCCTTTT+GGG | + | chr7.4:28715704-28715723 | MS.gene52241:CDS | 35.0% |
| CAGTTGAAGTGAAACCCAAA+AGG | - | chr7.4:28715721-28715740 | None:intergenic | 40.0% | |
| TGAGATCACTTGAGTGATGA+AGG | - | chr7.4:28715771-28715790 | None:intergenic | 40.0% | |
| AGCACAACGACACTGAGTAA+GGG | - | chr7.4:28715876-28715895 | None:intergenic | 45.0% | |
| GCAGGTATTACAACATGCTG+TGG | - | chr7.4:28715834-28715853 | None:intergenic | 45.0% | |
| ! | ACATGCTGTGGCTGTTGATT+TGG | - | chr7.4:28715822-28715841 | None:intergenic | 45.0% |
| CAACGACACTGAGTAAGGGT+TGG | - | chr7.4:28715872-28715891 | None:intergenic | 50.0% | |
| CAGCACAACGACACTGAGTA+AGG | - | chr7.4:28715877-28715896 | None:intergenic | 50.0% | |
| !! | GGGTTGGTGTTACTGAGCAA+AGG | - | chr7.4:28715856-28715875 | None:intergenic | 50.0% |
| !! | TGGTGTTACTGAGCAAAGGC+AGG | - | chr7.4:28715852-28715871 | None:intergenic | 50.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr7.4 | gene | 28715674 | 28715904 | 28715674 | ID=MS.gene52241 |
| chr7.4 | mRNA | 28715674 | 28715904 | 28715674 | ID=MS.gene52241.t1;Parent=MS.gene52241 |
| chr7.4 | exon | 28715674 | 28715904 | 28715674 | ID=MS.gene52241.t1.exon1;Parent=MS.gene52241.t1 |
| chr7.4 | CDS | 28715674 | 28715904 | 28715674 | ID=cds.MS.gene52241.t1;Parent=MS.gene52241.t1 |
| Gene Sequence |
| Protein sequence |
