AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene053678


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene053678.t1 MTR_4g106510 98.519 135 2 0 3 137 341 475 8.12e-93 278
MS.gene053678.t1 MTR_3g078550 83.704 135 22 0 3 137 311 445 4.91e-81 246
MS.gene053678.t1 MTR_3g078550 83.704 135 22 0 3 137 341 475 1.26e-80 246
MS.gene053678.t1 MTR_3g078490 81.481 135 25 0 3 137 371 505 9.44e-77 237
MS.gene053678.t1 MTR_6g086300 43.182 132 73 1 3 134 350 479 2.61e-35 127
MS.gene053678.t1 MTR_6g086300 43.182 132 73 1 3 134 350 479 2.61e-35 127
MS.gene053678.t1 MTR_8g083520 42.424 132 74 1 3 134 229 358 1.10e-34 124
MS.gene053678.t1 MTR_8g083520 42.424 132 74 1 3 134 309 438 5.04e-34 124
MS.gene053678.t1 MTR_6g086340 42.424 132 74 1 3 134 348 477 5.65e-34 124
MS.gene053678.t1 MTR_8g083530 42.424 132 74 1 3 134 351 480 1.25e-33 123
MS.gene053678.t1 MTR_8g064170 42.537 134 75 1 3 136 357 488 2.16e-30 114
MS.gene053678.t1 MTR_8g064170 42.537 134 75 1 3 136 389 520 2.71e-30 114
MS.gene053678.t1 MTR_3g078170 41.791 134 76 1 3 136 390 521 4.70e-29 111
MS.gene053678.t1 MTR_2g067660 42.857 133 74 1 5 137 374 504 2.94e-27 105
MS.gene053678.t1 MTR_4g107040 39.552 134 79 1 3 136 392 523 5.94e-27 105
MS.gene053678.t1 MTR_2g034160 36.765 136 72 4 5 131 381 511 1.01e-19 84.7
MS.gene053678.t1 MTR_1399s0020 36.429 140 79 3 1 137 195 327 8.85e-19 81.3
MS.gene053678.t1 MTR_2g042330 34.266 143 86 4 5 142 350 489 2.36e-12 63.5
MS.gene053678.t1 MTR_2g009750 34.951 103 62 2 5 106 339 437 8.92e-12 62.0
MS.gene053678.t1 MTR_2g009750 34.951 103 62 2 5 106 340 438 9.01e-12 62.0
MS.gene053678.t1 MTR_2g009750 34.286 105 61 3 5 106 340 439 1.95e-11 60.8
MS.gene053678.t1 MTR_4g093170 30.702 114 75 2 5 115 367 479 4.72e-11 60.1
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene053678.t1 AT1G74920 83.704 135 22 0 3 137 341 475 4.99e-82 250
MS.gene053678.t1 AT1G74920 83.704 135 22 0 3 137 336 470 5.28e-82 249
MS.gene053678.t1 AT3G48170 78.519 135 29 0 3 137 341 475 6.10e-77 237
MS.gene053678.t1 AT3G24503 46.212 132 69 1 3 134 349 478 9.61e-35 126
MS.gene053678.t1 AT1G79440 45.113 133 71 1 5 137 380 510 1.95e-28 109
MS.gene053678.t1 AT3G48000 39.706 136 80 1 1 136 384 517 2.42e-28 109
MS.gene053678.t1 AT1G23800 38.060 134 81 1 3 136 382 513 4.10e-28 108
MS.gene053678.t1 AT3G66658 36.691 139 74 4 5 134 381 514 6.47e-20 85.5
MS.gene053678.t1 AT3G66658 36.691 139 74 4 5 134 381 514 6.52e-20 85.5
MS.gene053678.t1 AT2G14170 28.000 150 100 4 5 149 439 585 3.21e-13 66.2
MS.gene053678.t1 AT1G54100 33.793 145 85 5 3 142 350 488 1.75e-12 63.9
MS.gene053678.t1 AT1G54100 33.793 145 85 5 3 142 350 488 1.75e-12 63.9
MS.gene053678.t1 AT2G14170 31.858 113 73 2 5 114 330 441 5.63e-12 62.8
MS.gene053678.t1 AT2G14170 31.858 113 73 2 5 114 439 550 6.44e-12 62.4

Find 26 sgRNAs with CRISPR-Local

Find 82 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TGCTCTGATGCAGGCATTTA+AGG 0.134214 5.1:-47633335 MS.gene053678:intron
ATTTGGAAAGAAGAAGTATT+TGG 0.232708 5.1:-47633657 MS.gene053678:CDS
TGACTACTTCCATGCAAATT+TGG 0.235483 5.1:-47633674 MS.gene053678:CDS
GGCATTAAACGTAGTGGTTT+TGG 0.252055 5.1:-47633256 MS.gene053678:CDS
CATTTAAGGCCGAAATAGTA+TGG 0.301924 5.1:-47633321 MS.gene053678:CDS
GCAATTGACCCATACTATTT+CGG 0.370412 5.1:+47633312 None:intergenic
ATGCTTCACTCAAGCGCCAT+GGG 0.461661 5.1:-47633281 MS.gene053678:CDS
GTCCTCTACAGCTATGGCTT+AGG 0.463601 5.1:-47633479 MS.gene053678:intron
GTCACATCAGTTATGATGGT+TGG 0.495328 5.1:+47633693 None:intergenic
TTCTTCTTTCCAAATTTGCA+TGG 0.496084 5.1:+47633665 None:intergenic
CAATGGCAGCATCTAAAGAA+AGG 0.502314 5.1:-47633729 MS.gene053678:intron
AAGATGTGAGCGTGTAACTA+AGG 0.508552 5.1:-47633429 MS.gene053678:intron
CATGCTTCACTCAAGCGCCA+TGG 0.516509 5.1:-47633282 MS.gene053678:CDS
AGTAGTCACATCAGTTATGA+TGG 0.526637 5.1:+47633689 None:intergenic
CATACCATTCTCCTAGTTCA+CGG 0.538382 5.1:+47633233 None:intergenic
TTGGCCGTGAACTAGGAGAA+TGG 0.556599 5.1:-47633237 MS.gene053678:intron
GACTGGTGGGTCTCGACCAG+AGG 0.561492 5.1:-47633832 MS.gene053678:intron
TTCACTCAAGCGCCATGGGG+AGG 0.564185 5.1:-47633277 MS.gene053678:CDS
GTCTCGAACGCTAAGAGTGA+GGG 0.574738 5.1:-47633867 MS.gene053678:CDS
TAGATCAATAGCTTCTTCCT+CGG 0.596377 5.1:+47633605 None:intergenic
TGTCTCGAACGCTAAGAGTG+AGG 0.614021 5.1:-47633868 MS.gene053678:CDS
ATTTAAGGCCGAAATAGTAT+GGG 0.622761 5.1:-47633320 MS.gene053678:CDS
TGGGGAGGCATTAAACGTAG+TGG 0.638861 5.1:-47633262 MS.gene053678:CDS
CTACGTTTAATGCCTCCCCA+TGG 0.639138 5.1:+47633265 None:intergenic
CATGGCGCTTGAGTGAAGCA+TGG 0.643282 5.1:+47633283 None:intergenic
TGCTTCACTCAAGCGCCATG+GGG 0.659834 5.1:-47633280 MS.gene053678:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! AGAAAAATAAATAAAAAGAT+AGG + chr5.1:47633415-47633434 None:intergenic 10.0%
!!! TTTGTTTAATATTTTTAACA+GGG - chr5.1:47633853-47633872 MS.gene053678:CDS 10.0%
!!! TTTTGTTTAATATTTTTAAC+AGG - chr5.1:47633852-47633871 MS.gene053678:CDS 10.0%
!!! TCTTTTTATTTATTTTTCTC+TGG - chr5.1:47633416-47633435 MS.gene053678:intron 15.0%
!!! TTGTTTAATATTTTTAACAG+GGG - chr5.1:47633854-47633873 MS.gene053678:CDS 15.0%
!! AAAAGAAACCCATAATTATA+AGG - chr5.1:47633470-47633489 MS.gene053678:CDS 20.0%
!! AAATTGGAATTATACATGAT+AGG - chr5.1:47633637-47633656 MS.gene053678:CDS 20.0%
!! AATAAAAAGATAGGCATTTA+AGG + chr5.1:47633406-47633425 None:intergenic 20.0%
!! AATTGGAATTATACATGATA+GGG - chr5.1:47633638-47633657 MS.gene053678:CDS 20.0%
!! ATTAAACAAAATCAGACTTA+AGG + chr5.1:47633843-47633862 None:intergenic 20.0%
!! CAAAACATAATCAATTATCA+TGG + chr5.1:47632824-47632843 None:intergenic 20.0%
!! CAAGAAAACAACTAAATAAT+TGG - chr5.1:47633193-47633212 MS.gene053678:intron 20.0%
! AGACTTCACCTTATAATTAT+GGG + chr5.1:47633481-47633500 None:intergenic 25.0%
! GGTATCTAAGTTATATACTT+TGG - chr5.1:47633078-47633097 MS.gene053678:intron 25.0%
! TAGACTTCACCTTATAATTA+TGG + chr5.1:47633482-47633501 None:intergenic 25.0%
!! ATTATGTTTTGCAAATGCAA+TGG - chr5.1:47632833-47632852 MS.gene053678:intron 25.0%
!!! ATTTGGAAAGAAGAAGTATT+TGG - chr5.1:47632922-47632941 MS.gene053678:intron 25.0%
ACAGTCAATAAAGCTATGAA+CGG - chr5.1:47633583-47633602 MS.gene053678:intron 30.0%
TCTGACATTAATACAGGTTT+AGG + chr5.1:47633775-47633794 None:intergenic 30.0%
TTCTTCTTTCCAAATTTGCA+TGG + chr5.1:47632917-47632936 None:intergenic 30.0%
! AATTCCAATTTTACCATGCA+AGG + chr5.1:47633628-47633647 None:intergenic 30.0%
! ATTTAAGGCCGAAATAGTAT+GGG - chr5.1:47633259-47633278 MS.gene053678:CDS 30.0%
! TTCATCTGTATACTCTTTTC+CGG - chr5.1:47633042-47633061 MS.gene053678:intron 30.0%
!! TTATTTTTCTCTGGATGAAG+AGG - chr5.1:47633425-47633444 MS.gene053678:intron 30.0%
ACCTGTATTAATGTCAGATC+TGG - chr5.1:47633777-47633796 MS.gene053678:intron 35.0%
ACTAAGGTGAGACTGATTAT+AGG - chr5.1:47633166-47633185 MS.gene053678:intron 35.0%
AGTAGTCACATCAGTTATGA+TGG + chr5.1:47632893-47632912 None:intergenic 35.0%
ATGTTTCCTTGGTCTGAATT+TGG - chr5.1:47633685-47633704 MS.gene053678:CDS 35.0%
ATTTGGCATAAAGGGATAGT+TGG - chr5.1:47633702-47633721 MS.gene053678:CDS 35.0%
CATAAAGGGATAGTTGGATT+GGG - chr5.1:47633708-47633727 MS.gene053678:CDS 35.0%
CATTCCTTGCATGGTAAAAT+TGG - chr5.1:47633621-47633640 MS.gene053678:CDS 35.0%
GACTTAAGGTCAAATAAGAC+AGG + chr5.1:47633829-47633848 None:intergenic 35.0%
GCAATTGACCCATACTATTT+CGG + chr5.1:47633270-47633289 None:intergenic 35.0%
TAGATCAATAGCTTCTTCCT+CGG + chr5.1:47632977-47632996 None:intergenic 35.0%
TGACTACTTCCATGCAAATT+TGG - chr5.1:47632905-47632924 MS.gene053678:intron 35.0%
TGATAGGGATTAACATGTGT+CGG - chr5.1:47633653-47633672 MS.gene053678:CDS 35.0%
TGGTCTGAATTTGGCATAAA+GGG - chr5.1:47633694-47633713 MS.gene053678:CDS 35.0%
TTGGTCTGAATTTGGCATAA+AGG - chr5.1:47633693-47633712 MS.gene053678:CDS 35.0%
TTTATGCCAAATTCAGACCA+AGG + chr5.1:47633694-47633713 None:intergenic 35.0%
! AACGTTTTTACACACAGAAC+AGG + chr5.1:47632949-47632968 None:intergenic 35.0%
! AGAAAGCATAATCATACCTC+TGG + chr5.1:47632766-47632785 None:intergenic 35.0%
! CATTTAAGGCCGAAATAGTA+TGG - chr5.1:47633258-47633277 MS.gene053678:CDS 35.0%
AAGATGTGAGCGTGTAACTA+AGG - chr5.1:47633150-47633169 MS.gene053678:intron 40.0%
AGTTTATCTTGCTCTGATGC+AGG - chr5.1:47633235-47633254 MS.gene053678:intron 40.0%
ATTAATGTCAGATCTGGCGT+GGG - chr5.1:47633783-47633802 MS.gene053678:intron 40.0%
CAATGGCAGCATCTAAAGAA+AGG - chr5.1:47632850-47632869 MS.gene053678:intron 40.0%
CATACCATTCTCCTAGTTCA+CGG + chr5.1:47633349-47633368 None:intergenic 40.0%
GATACCATTCCACAAATGAC+CGG + chr5.1:47633064-47633083 None:intergenic 40.0%
GCATAAAGGGATAGTTGGAT+TGG - chr5.1:47633707-47633726 MS.gene053678:CDS 40.0%
GCCAGATCTGACATTAATAC+AGG + chr5.1:47633781-47633800 None:intergenic 40.0%
GTCACATCAGTTATGATGGT+TGG + chr5.1:47632889-47632908 None:intergenic 40.0%
TAAGGTGAAGTCTAAGTCTG+TGG - chr5.1:47633488-47633507 MS.gene053678:intron 40.0%
TATTAATGTCAGATCTGGCG+TGG - chr5.1:47633782-47633801 MS.gene053678:intron 40.0%
TGTAAAAACGTTTAGCACCG+AGG - chr5.1:47632957-47632976 MS.gene053678:intron 40.0%
! GGCATTAAACGTAGTGGTTT+TGG - chr5.1:47633323-47633342 MS.gene053678:CDS 40.0%
! TACTCTTTTCCGGTCATTTG+TGG - chr5.1:47633052-47633071 MS.gene053678:intron 40.0%
! TTTTCCGGTCATTTGTGGAA+TGG - chr5.1:47633057-47633076 MS.gene053678:intron 40.0%
ACTTTGGTCCTCTACAGCTA+TGG - chr5.1:47633094-47633113 MS.gene053678:intron 45.0%
ATGATCACTACACTGTCAGC+AGG + chr5.1:47633535-47633554 None:intergenic 45.0%
CTATGAACGGATCATGACAC+TGG - chr5.1:47633596-47633615 MS.gene053678:CDS 45.0%
CTGTGCAAGTGTGTTCAGTT+AGG - chr5.1:47632784-47632803 MS.gene053678:intron 45.0%
GGCACATTTGCATGTTTCCT+TGG - chr5.1:47633674-47633693 MS.gene053678:CDS 45.0%
TGATCACTACACTGTCAGCA+GGG + chr5.1:47633534-47633553 None:intergenic 45.0%
TGCTCTGATGCAGGCATTTA+AGG - chr5.1:47633244-47633263 MS.gene053678:CDS 45.0%
!!! GTGCGACAATTTTGACTGGT+GGG - chr5.1:47632734-47632753 MS.gene053678:intron 45.0%
ACACTGGTGCATTCCTTGCA+TGG - chr5.1:47633612-47633631 MS.gene053678:CDS 50.0%
ATGCTTCACTCAAGCGCCAT+GGG - chr5.1:47633298-47633317 MS.gene053678:CDS 50.0%
CACCTAAGCCATAGCTGTAG+AGG + chr5.1:47633105-47633124 None:intergenic 50.0%
CTACGTTTAATGCCTCCCCA+TGG + chr5.1:47633317-47633336 None:intergenic 50.0%
GTCCTCTACAGCTATGGCTT+AGG - chr5.1:47633100-47633119 MS.gene053678:intron 50.0%
GTCTCGAACGCTAAGAGTGA+GGG - chr5.1:47632712-47632731 MS.gene053678:intron 50.0%
TGTCTCGAACGCTAAGAGTG+AGG - chr5.1:47632711-47632730 MS.gene053678:intron 50.0%
TTGGCCGTGAACTAGGAGAA+TGG - chr5.1:47633342-47633361 MS.gene053678:intron 50.0%
!! GAGGGTGCGACAATTTTGAC+TGG - chr5.1:47632730-47632749 MS.gene053678:intron 50.0%
!! TGGGGAGGCATTAAACGTAG+TGG - chr5.1:47633317-47633336 MS.gene053678:CDS 50.0%
!!! AGTGGTTTTGGCCGTGAACT+AGG - chr5.1:47633335-47633354 MS.gene053678:intron 50.0%
!!! GGTGCGACAATTTTGACTGG+TGG - chr5.1:47632733-47632752 MS.gene053678:intron 50.0%
CATGCTTCACTCAAGCGCCA+TGG - chr5.1:47633297-47633316 MS.gene053678:CDS 55.0%
CATGGCGCTTGAGTGAAGCA+TGG + chr5.1:47633299-47633318 None:intergenic 55.0%
TGCTTCACTCAAGCGCCATG+GGG - chr5.1:47633299-47633318 MS.gene053678:CDS 55.0%
TTCACTCAAGCGCCATGGGG+AGG - chr5.1:47633302-47633321 MS.gene053678:CDS 60.0%
! GACTGGTGGGTCTCGACCAG+AGG - chr5.1:47632747-47632766 MS.gene053678:intron 65.0%


Chromosome Type Strat End Strand Name
chr5.1 gene 47632682 47633919 47632682 ID=MS.gene053678
chr5.1 mRNA 47632682 47633919 47632682 ID=MS.gene053678.t1;Parent=MS.gene053678
chr5.1 exon 47633833 47633919 47633833 ID=MS.gene053678.t1.exon1;Parent=MS.gene053678.t1
chr5.1 CDS 47633833 47633919 47633833 ID=cds.MS.gene053678.t1;Parent=MS.gene053678.t1
chr5.1 exon 47633591 47633742 47633591 ID=MS.gene053678.t1.exon2;Parent=MS.gene053678.t1
chr5.1 CDS 47633591 47633742 47633591 ID=cds.MS.gene053678.t1;Parent=MS.gene053678.t1
chr5.1 exon 47633430 47633490 47633430 ID=MS.gene053678.t1.exon3;Parent=MS.gene053678.t1
chr5.1 CDS 47633430 47633490 47633430 ID=cds.MS.gene053678.t1;Parent=MS.gene053678.t1
chr5.1 exon 47633238 47633344 47633238 ID=MS.gene053678.t1.exon4;Parent=MS.gene053678.t1
chr5.1 CDS 47633238 47633344 47633238 ID=cds.MS.gene053678.t1;Parent=MS.gene053678.t1
chr5.1 exon 47632682 47632727 47632682 ID=MS.gene053678.t1.exon5;Parent=MS.gene053678.t1
chr5.1 CDS 47632682 47632727 47632682 ID=cds.MS.gene053678.t1;Parent=MS.gene053678.t1
Gene Sequence

>MS.gene053678

ATGGTTCAGTACGAAAAAATATTGAAGTTTGTCTCGAACGCTAAGAGTGAGGGTGCGACAATTTTGACTGGTGGGTCTCGACCAGAGGTATGATTATGCTTTCTGTGCAAGTGTGTTCAGTTAGGTATTTCTGCATTTTCCATGATAATTGATTATGTTTTGCAAATGCAATGGCAGCATCTAAAGAAAGGATTCTTTGTTGAACCAACCATCATAACTGATGTGACTACTTCCATGCAAATTTGGAAAGAAGAAGTATTTGGACCTGTTCTGTGTGTAAAAACGTTTAGCACCGAGGAAGAAGCTATTGATCTAGCAAATGACACTATGTGAGTCAGTTTTCTGCGAACTAACGTTGACTTCATCTGTATACTCTTTTCCGGTCATTTGTGGAATGGTATCTAAGTTATATACTTTGGTCCTCTACAGCTATGGCTTAGGTGCTGCTGTAATATCAAATGATCTAGAAAGATGTGAGCGTGTAACTAAGGTGAGACTGATTATAGGTCATCAAGAAAACAACTAAATAATTGGATTGTTGTTGCAATTGAATAGTTTATCTTGCTCTGATGCAGGCATTTAAGGCCGAAATAGTATGGGTCAATTGCTGTCAGCCATGCTTCACTCAAGCGCCATGGGGAGGCATTAAACGTAGTGGTTTTGGCCGTGAACTAGGAGAATGGTATGACTCTATTTACTAAATTAGCTAATGTGAAAGTCTCCTTAAATGCCTATCTTTTTATTTATTTTTCTCTGGATGAAGAGGTGATCGCAGCTTCTTAATATGCAAAAGAAACCCATAATTATAAGGTGAAGTCTAAGTCTGTGGATTGTTAAATGTAGTACATTCCCTGCTGACAGTGTAGTGATCATCAGATACTGTACTTCTAAATTCAATTTAACAGTCAATAAAGCTATGAACGGATCATGACACTGGTGCATTCCTTGCATGGTAAAATTGGAATTATACATGATAGGGATTAACATGTGTCGGCACATTTGCATGTTTCCTTGGTCTGAATTTGGCATAAAGGGATAGTTGGATTGGGTAGACAATTAACTGTTACACAATCGCTGTAGAGACTACATACCTAAACCTGTATTAATGTCAGATCTGGCGTGGGAAATAAACAATTTCGCTAAACCTGTCTTATTTGACCTTAAGTCTGATTTTGTTTAATATTTTTAACAGGGGACAATTACTTGAGCGTGAAGCAAGTGACTCAGTACATCTCTGA

Protein sequence

>MS.gene053678.t1

MVQYEKILKFVSNAKSEGATILTGGSRPEHLKKGFFVEPTIITDVTTSMQIWKEEVFGPVLCVKTFSTEEEAIDLANDTIYGLGAAVISNDLERCERVTKAFKAEIVWVNCCQPCFTQAPWGGIKRSGFGRELGEWGQLLEREASDSVHL