Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene52267.t1 | XP_003598099.1 | 100 | 147 | 0 | 0 | 2 | 148 | 1 | 147 | 5.90E-70 | 273.5 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene52267.t1 | Q9LJ98 | 70.7 | 150 | 39 | 3 | 2 | 148 | 1 | 148 | 3.9e-46 | 185.7 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene52267.t1 | G7J0A7 | 100.0 | 147 | 0 | 0 | 2 | 148 | 1 | 147 | 4.2e-70 | 273.5 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene053006 | MS.gene52267 | 0.812046 | 5.16E-51 | -1.69E-46 |
| MS.gene055778 | MS.gene52267 | 0.809805 | 1.58E-50 | -1.69E-46 |
| MS.gene05795 | MS.gene52267 | 0.802117 | 6.53E-49 | -1.69E-46 |
| MS.gene058037 | MS.gene52267 | 0.801233 | 9.92E-49 | -1.69E-46 |
| MS.gene060429 | MS.gene52267 | 0.810604 | 1.06E-50 | -1.69E-46 |
| MS.gene060586 | MS.gene52267 | 0.810395 | 1.18E-50 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Find 45 sgRNAs with CRISPR-Local
Find 43 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| CATTTCAAGTTCCGTTATTT+TGG | 0.192430 | 3.1:-2710534 | None:intergenic |
| GCAACTGCTTGTTCATTTAC+TGG | 0.248625 | 3.1:-2710467 | None:intergenic |
| TACTGGCTCCTTGCGATCTT+CGG | 0.331035 | 3.1:-2710450 | None:intergenic |
| CAAAATAACGGAACTTGAAA+TGG | 0.346568 | 3.1:+2710535 | MS.gene52267:CDS |
| CGGCGTCTGCCTTCCTCATC+CGG | 0.403003 | 3.1:-2710781 | None:intergenic |
| TATTCATTTGTGTAGAATCA+TGG | 0.403780 | 3.1:+2710418 | None:intergenic |
| AGACATTGGAAAAGAAGAAA+AGG | 0.418775 | 3.1:+2710726 | MS.gene52267:CDS |
| GGAGTACTTGTGGGCCCTGC+GGG | 0.425415 | 3.1:+2710845 | MS.gene52267:CDS |
| AGCAGGCAAGACCTCTTTGA+TGG | 0.434394 | 3.1:-2710654 | None:intergenic |
| AGACGATGCTATCGAATGAT+TGG | 0.440454 | 3.1:+2710611 | MS.gene52267:CDS |
| TTCGATAGCATCGTCTAGAT+TGG | 0.460560 | 3.1:-2710604 | None:intergenic |
| TGTCATTCGCTGCCACCCGC+AGG | 0.461645 | 3.1:-2710860 | None:intergenic |
| TCTTAATGCCATTCAGCCGC+TGG | 0.472683 | 3.1:+2710580 | MS.gene52267:CDS |
| AGATTGGTCCAGCGGCTGAA+TGG | 0.484859 | 3.1:-2710588 | None:intergenic |
| TCTTTATTTCGCTGCACAGC+AGG | 0.487561 | 3.1:-2710671 | None:intergenic |
| TTGAGACTAAATATAAAATC+CGG | 0.490885 | 3.1:+2710762 | MS.gene52267:CDS |
| GACATTGGAAAAGAAGAAAA+GGG | 0.500685 | 3.1:+2710727 | MS.gene52267:CDS |
| GTGTAGAATCATGGCTAGTA+AGG | 0.508954 | 3.1:+2710427 | None:intergenic |
| GCCGAGGTGAATGATGAATC+TGG | 0.515159 | 3.1:+2710800 | MS.gene52267:CDS |
| GAATGATGAATCTGGTAAGA+AGG | 0.524633 | 3.1:+2710808 | MS.gene52267:CDS |
| CCAAATTTACACCAAAATAA+CGG | 0.527386 | 3.1:+2710523 | MS.gene52267:CDS |
| GTACTTGTGGGCCCTGCGGG+TGG | 0.538420 | 3.1:+2710848 | MS.gene52267:CDS |
| TGCGAGGCTTAACGAGACAT+TGG | 0.548123 | 3.1:+2710712 | MS.gene52267:CDS |
| GTCATTCGCTGCCACCCGCA+GGG | 0.549383 | 3.1:-2710859 | None:intergenic |
| TCAGCTCAAGGAGTACTTGT+GGG | 0.554339 | 3.1:+2710836 | MS.gene52267:CDS |
| AAGAAGGAAGGTTCAGCTCA+AGG | 0.558366 | 3.1:+2710824 | MS.gene52267:CDS |
| CGATGCTATCGAATGATTGG+AGG | 0.563926 | 3.1:+2710614 | MS.gene52267:CDS |
| GATGAATCTGGTAAGAAGGA+AGG | 0.576871 | 3.1:+2710812 | MS.gene52267:CDS |
| ATATAAAATCCGGATGAGGA+AGG | 0.583660 | 3.1:+2710772 | MS.gene52267:CDS |
| GCTGTGCAGCGAAATAAAGA+AGG | 0.585102 | 3.1:+2710674 | MS.gene52267:CDS |
| CTAAATATAAAATCCGGATG+AGG | 0.587775 | 3.1:+2710768 | MS.gene52267:CDS |
| TAGTAAGGCCGAAGATCGCA+AGG | 0.597879 | 3.1:+2710442 | MS.gene52267:CDS |
| GGAAGTCAGCGAGCACTCGT+TGG | 0.600111 | 3.1:+2710556 | MS.gene52267:CDS |
| AGGAGTACTTGTGGGCCCTG+CGG | 0.600783 | 3.1:+2710844 | MS.gene52267:CDS |
| TTCAGCTCAAGGAGTACTTG+TGG | 0.603923 | 3.1:+2710835 | MS.gene52267:CDS |
| AATGATTGGAGGGGTGCTTG+TGG | 0.605277 | 3.1:+2710625 | MS.gene52267:CDS |
| AAATAAAGAAGGACTTGAAG+AGG | 0.608952 | 3.1:+2710685 | MS.gene52267:CDS |
| GATGCTATCGAATGATTGGA+GGG | 0.617244 | 3.1:+2710615 | MS.gene52267:CDS |
| ACCAGATTCATCATTCACCT+CGG | 0.628665 | 3.1:-2710801 | None:intergenic |
| GACTTGAAGAGGTTGTTGCG+AGG | 0.642407 | 3.1:+2710696 | MS.gene52267:CDS |
| CATCGTCTAGATTGGTCCAG+CGG | 0.650779 | 3.1:-2710596 | None:intergenic |
| GATGAGGAAGGCAGACGCCG+AGG | 0.652733 | 3.1:+2710784 | MS.gene52267:CDS |
| CAAATATGTATGCTGCTATG+AGG | 0.656265 | 3.1:+2710489 | MS.gene52267:CDS |
| TGTGGAAAGAACCATCAAAG+AGG | 0.689890 | 3.1:+2710643 | MS.gene52267:CDS |
| ATGCTATCGAATGATTGGAG+GGG | 0.721195 | 3.1:+2710616 | MS.gene52267:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | TTGAGACTAAATATAAAATC+CGG | + | chr3.1:2710762-2710781 | MS.gene52267:CDS | 20.0% |
| ! | CCAAATTTACACCAAAATAA+CGG | + | chr3.1:2710523-2710542 | MS.gene52267:CDS | 25.0% |
| AAATAAAGAAGGACTTGAAG+AGG | + | chr3.1:2710685-2710704 | MS.gene52267:CDS | 30.0% | |
| AGACATTGGAAAAGAAGAAA+AGG | + | chr3.1:2710726-2710745 | MS.gene52267:CDS | 30.0% | |
| CTAAATATAAAATCCGGATG+AGG | + | chr3.1:2710768-2710787 | MS.gene52267:CDS | 30.0% | |
| GACATTGGAAAAGAAGAAAA+GGG | + | chr3.1:2710727-2710746 | MS.gene52267:CDS | 30.0% | |
| ! | CAAAATAACGGAACTTGAAA+TGG | + | chr3.1:2710535-2710554 | MS.gene52267:CDS | 30.0% |
| !! | TTTTGGTGTAAATTTGGTTG+AGG | - | chr3.1:2710520-2710539 | None:intergenic | 30.0% |
| !!! | CATTTCAAGTTCCGTTATTT+TGG | - | chr3.1:2710537-2710556 | None:intergenic | 30.0% |
| !!! | CCGTTATTTTGGTGTAAATT+TGG | - | chr3.1:2710526-2710545 | None:intergenic | 30.0% |
| ATATAAAATCCGGATGAGGA+AGG | + | chr3.1:2710772-2710791 | MS.gene52267:CDS | 35.0% | |
| CAAATATGTATGCTGCTATG+AGG | + | chr3.1:2710489-2710508 | MS.gene52267:CDS | 35.0% | |
| GAATGATGAATCTGGTAAGA+AGG | + | chr3.1:2710808-2710827 | MS.gene52267:CDS | 35.0% | |
| ACCAGATTCATCATTCACCT+CGG | - | chr3.1:2710804-2710823 | None:intergenic | 40.0% | |
| AGACGATGCTATCGAATGAT+TGG | + | chr3.1:2710611-2710630 | MS.gene52267:CDS | 40.0% | |
| ATGCTATCGAATGATTGGAG+GGG | + | chr3.1:2710616-2710635 | MS.gene52267:CDS | 40.0% | |
| GATGAATCTGGTAAGAAGGA+AGG | + | chr3.1:2710812-2710831 | MS.gene52267:CDS | 40.0% | |
| GATGCTATCGAATGATTGGA+GGG | + | chr3.1:2710615-2710634 | MS.gene52267:CDS | 40.0% | |
| GCAACTGCTTGTTCATTTAC+TGG | - | chr3.1:2710470-2710489 | None:intergenic | 40.0% | |
| TGTGGAAAGAACCATCAAAG+AGG | + | chr3.1:2710643-2710662 | MS.gene52267:CDS | 40.0% | |
| TTCGATAGCATCGTCTAGAT+TGG | - | chr3.1:2710607-2710626 | None:intergenic | 40.0% | |
| AAGAAGGAAGGTTCAGCTCA+AGG | + | chr3.1:2710824-2710843 | MS.gene52267:CDS | 45.0% | |
| CGATGCTATCGAATGATTGG+AGG | + | chr3.1:2710614-2710633 | MS.gene52267:CDS | 45.0% | |
| GCTGTGCAGCGAAATAAAGA+AGG | + | chr3.1:2710674-2710693 | MS.gene52267:CDS | 45.0% | |
| ! | TCTTTATTTCGCTGCACAGC+AGG | - | chr3.1:2710674-2710693 | None:intergenic | 45.0% |
| !! | TCAGCTCAAGGAGTACTTGT+GGG | + | chr3.1:2710836-2710855 | MS.gene52267:CDS | 45.0% |
| !! | TTCAGCTCAAGGAGTACTTG+TGG | + | chr3.1:2710835-2710854 | MS.gene52267:CDS | 45.0% |
| AGCAGGCAAGACCTCTTTGA+TGG | - | chr3.1:2710657-2710676 | None:intergenic | 50.0% | |
| GACTTGAAGAGGTTGTTGCG+AGG | + | chr3.1:2710696-2710715 | MS.gene52267:CDS | 50.0% | |
| GCCGAGGTGAATGATGAATC+TGG | + | chr3.1:2710800-2710819 | MS.gene52267:CDS | 50.0% | |
| TAGTAAGGCCGAAGATCGCA+AGG | + | chr3.1:2710442-2710461 | MS.gene52267:CDS | 50.0% | |
| TCTTAATGCCATTCAGCCGC+TGG | + | chr3.1:2710580-2710599 | MS.gene52267:CDS | 50.0% | |
| TGCGAGGCTTAACGAGACAT+TGG | + | chr3.1:2710712-2710731 | MS.gene52267:CDS | 50.0% | |
| ! | CATCGTCTAGATTGGTCCAG+CGG | - | chr3.1:2710599-2710618 | None:intergenic | 50.0% |
| !! | AATGATTGGAGGGGTGCTTG+TGG | + | chr3.1:2710625-2710644 | MS.gene52267:CDS | 50.0% |
| !! | TACTGGCTCCTTGCGATCTT+CGG | - | chr3.1:2710453-2710472 | None:intergenic | 50.0% |
| ! | AGATTGGTCCAGCGGCTGAA+TGG | - | chr3.1:2710591-2710610 | None:intergenic | 55.0% |
| GGAAGTCAGCGAGCACTCGT+TGG | + | chr3.1:2710556-2710575 | MS.gene52267:CDS | 60.0% | |
| !! | AGGAGTACTTGTGGGCCCTG+CGG | + | chr3.1:2710844-2710863 | MS.gene52267:CDS | 60.0% |
| CGGCGTCTGCCTTCCTCATC+CGG | - | chr3.1:2710784-2710803 | None:intergenic | 65.0% | |
| GATGAGGAAGGCAGACGCCG+AGG | + | chr3.1:2710784-2710803 | MS.gene52267:CDS | 65.0% | |
| ! | GGAGTACTTGTGGGCCCTGC+GGG | + | chr3.1:2710845-2710864 | MS.gene52267:CDS | 65.0% |
| GTACTTGTGGGCCCTGCGGG+TGG | + | chr3.1:2710848-2710867 | MS.gene52267:CDS | 70.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr3.1 | gene | 2710433 | 2710880 | 2710433 | ID=MS.gene52267 |
| chr3.1 | mRNA | 2710433 | 2710880 | 2710433 | ID=MS.gene52267.t1;Parent=MS.gene52267 |
| chr3.1 | exon | 2710433 | 2710880 | 2710433 | ID=MS.gene52267.t1.exon1;Parent=MS.gene52267.t1 |
| chr3.1 | CDS | 2710433 | 2710880 | 2710433 | ID=cds.MS.gene52267.t1;Parent=MS.gene52267.t1 |
| Gene Sequence |
| Protein sequence |
