AlfalfaGEDB | MS.gene52279

M. sativa cultivar XinJiangDaYe / MS.gene52279

KEGG: -
GO: -
PFAM: PF13891.9

Nr:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene52279.t1	XP_003624562.1	85.1	148	18	1	1	144	1	148	7.50E-62	246.5

Swissprot:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score

Trembl:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene52279.t1	G7KYC9	85.1	148	18	1	1	144	1	148	5.4e-62	246.5

TFs/TRs:

Gene ID	Type	Classification

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network:

Gene1	Gene2	correlation coefficient	p_value	FDR
MS.gene055555	MS.gene52279	-0.804603	2.00E-49	-1.69E-46

PPI:

Gene1	Gene2	Type

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene52279.t1	MTR_7g084890	90.541	148	10	1	1	144	1	148	1.69e-74	222
MS.gene52279.t1	MTR_1g116580	39.200	125	63	2	18	142	14	125	2.19e-24	94.0

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene52279.t1	AT1G05860	46.951	164	66	4	1	145	32	193	7.01e-38	130
MS.gene52279.t1	AT1G05860	46.951	164	66	4	1	145	32	193	7.01e-38	130
MS.gene52279.t1	AT1G05860	46.626	163	66	4	1	144	32	192	1.19e-37	128
MS.gene52279.t1	AT2G31600	44.099	161	65	5	8	145	56	214	9.37e-37	125
MS.gene52279.t1	AT2G31600	44.099	161	65	5	8	145	56	214	9.37e-37	125
MS.gene52279.t1	AT3G53860	45.333	150	71	2	7	145	45	194	6.10e-36	125
MS.gene52279.t1	AT2G31600	44.099	161	65	5	8	145	56	214	1.39e-35	125
MS.gene52279.t1	AT2G31600	44.099	161	65	5	8	145	56	214	1.39e-35	125
MS.gene52279.t1	AT2G31600	44.099	161	65	5	8	145	56	214	1.39e-35	125
MS.gene52279.t1	AT2G31600	44.099	161	65	5	8	145	56	214	1.39e-35	125

Find 42 sgRNAs with CRISPR-Local

Find 42 sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
GCTGTTACCGAGGTCATTAC+TGG	0.271359	7.3:-23734299	MS.gene52279:CDS
AGGTCATTACTGGGCGTTAA+TGG	0.298106	7.3:-23734289	MS.gene52279:CDS
GCTGCTGCTGCTGTTGATAA+TGG	0.339422	7.3:-23734168	MS.gene52279:CDS
GGAAGATGAAGAAAGAAATT+CGG	0.342169	7.3:-23734208	MS.gene52279:CDS
CTAAGTACAGAGAGTATTAC+TGG	0.348566	7.3:-23734254	MS.gene52279:CDS
GTTGCACCGCCGTGGCAAAA+AGG	0.349754	7.3:-23734035	MS.gene52279:CDS
AGAGAGTATTACTGGACTTA+TGG	0.365244	7.3:-23734246	MS.gene52279:CDS
GAAGATGAAGAAAGAAATTC+GGG	0.400485	7.3:-23734207	MS.gene52279:CDS
CCACGATAAAGCTTCTGATT+AGG	0.415729	7.3:+23734057	None:intergenic
CACGGCGGAGGCGGCGGACG+AGG	0.423786	7.3:+23734337	None:intergenic
GTCTGACACGGCGGAGGCGG+CGG	0.433953	7.3:+23734331	None:intergenic
GACCTCGCGGCGGGTTAAAA+CGG	0.448768	7.3:+23734359	None:intergenic
TGCTGTTGAGAGTCTTCCAT+TGG	0.466609	7.3:+23734450	None:intergenic
CTTTCTTCATCTTCCTTGAA+CGG	0.468778	7.3:+23734216	None:intergenic
GTTGTCTGACACGGCGGAGG+CGG	0.468951	7.3:+23734328	None:intergenic
GGCGTTAATGGAAGAGCTTA+AGG	0.485899	7.3:-23734277	MS.gene52279:CDS
ATAATGGTGATGACTTTGTT+CGG	0.491789	7.3:-23734152	MS.gene52279:CDS
GCGGACGAGGACCTCGCGGC+GGG	0.498422	7.3:+23734350	None:intergenic
TTATGGAAGGAGTCCGTTCA+AGG	0.519406	7.3:-23734229	MS.gene52279:CDS
TCGGCACCGTCGATTGTCAT+CGG	0.530875	7.3:+23734408	None:intergenic
GTCATCGGCGCGATTTGTTG+CGG	0.555912	7.3:+23734423	None:intergenic
CTGTTACCGAGGTCATTACT+GGG	0.578965	7.3:-23734298	MS.gene52279:CDS
CTTAGTAAGAGCCATAGCCT+TGG	0.581621	7.3:+23734107	None:intergenic
TTTGTTCGGTGTGCTTCTAG+TGG	0.584923	7.3:-23734138	MS.gene52279:CDS
AGAGTTGTCTGACACGGCGG+AGG	0.587249	7.3:+23734325	None:intergenic
TGGTTGCAAGACCAAGGCTA+TGG	0.587740	7.3:-23734118	MS.gene52279:CDS
CAACTCTCTCGCTGTTACCG+AGG	0.590857	7.3:-23734309	MS.gene52279:CDS
AGTATTACTGGACTTATGGA+AGG	0.608751	7.3:-23734242	MS.gene52279:CDS
GGCGGACGAGGACCTCGCGG+CGG	0.609662	7.3:+23734349	None:intergenic
AGCGAGGGCTAAGTCTTCGT+CGG	0.616825	7.3:+23734389	None:intergenic
TTATCGTGGTTGCACCGCCG+TGG	0.625527	7.3:-23734043	MS.gene52279:CDS
TTCTAGTGGTTGCAAGACCA+AGG	0.627196	7.3:-23734124	MS.gene52279:CDS
ATCGGCGCGATTTGTTGCGG+TGG	0.628254	7.3:+23734426	None:intergenic
GGCGGCGGACGAGGACCTCG+CGG	0.628435	7.3:+23734346	None:intergenic
CTCGCGGCGGGTTAAAACGG+AGG	0.662601	7.3:+23734362	None:intergenic
ATCGCGCCGATGACAATCGA+CGG	0.665199	7.3:-23734414	MS.gene52279:CDS
CCTAATCAGAAGCTTTATCG+TGG	0.666724	7.3:-23734057	MS.gene52279:CDS
TTAAAACGGAGGAGTTAGCG+AGG	0.671046	7.3:+23734373	None:intergenic
AGCGAGAGAGTTGTCTGACA+CGG	0.684863	7.3:+23734319	None:intergenic
TAAAACGGAGGAGTTAGCGA+GGG	0.688548	7.3:+23734374	None:intergenic
GAGAGAGTTGTCTGACACGG+CGG	0.692611	7.3:+23734322	None:intergenic
TTAACGCCCAGTAATGACCT+CGG	0.702952	7.3:+23734292	None:intergenic

CRISPR-GE

badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content
	GAAGATGAAGAAAGAAATTC+GGG	-	chr7.3:23734272-23734291	MS.gene52279:CDS	30.0%
	GGAAGATGAAGAAAGAAATT+CGG	-	chr7.3:23734271-23734290	MS.gene52279:CDS	30.0%
!	ATAATGGTGATGACTTTGTT+CGG	-	chr7.3:23734327-23734346	MS.gene52279:CDS	30.0%
	AGTATTACTGGACTTATGGA+AGG	-	chr7.3:23734237-23734256	MS.gene52279:CDS	35.0%
	CTTTCTTCATCTTCCTTGAA+CGG	+	chr7.3:23734266-23734285	None:intergenic	35.0%
!!	AGAGAGTATTACTGGACTTA+TGG	-	chr7.3:23734233-23734252	MS.gene52279:CDS	35.0%
!!	CTAAGTACAGAGAGTATTAC+TGG	-	chr7.3:23734225-23734244	MS.gene52279:CDS	35.0%
	CCACGATAAAGCTTCTGATT+AGG	+	chr7.3:23734425-23734444	None:intergenic	40.0%
!	CCTAATCAGAAGCTTTATCG+TGG	-	chr7.3:23734422-23734441	MS.gene52279:CDS	40.0%
	AGGTCATTACTGGGCGTTAA+TGG	-	chr7.3:23734190-23734209	MS.gene52279:CDS	45.0%
	CTGTTACCGAGGTCATTACT+GGG	-	chr7.3:23734181-23734200	MS.gene52279:CDS	45.0%
	CTTAGTAAGAGCCATAGCCT+TGG	+	chr7.3:23734375-23734394	None:intergenic	45.0%
	GGCGTTAATGGAAGAGCTTA+AGG	-	chr7.3:23734202-23734221	MS.gene52279:CDS	45.0%
	TAAAACGGAGGAGTTAGCGA+GGG	+	chr7.3:23734108-23734127	None:intergenic	45.0%
	TTAAAACGGAGGAGTTAGCG+AGG	+	chr7.3:23734109-23734128	None:intergenic	45.0%
	TTAACGCCCAGTAATGACCT+CGG	+	chr7.3:23734190-23734209	None:intergenic	45.0%
	TTATGGAAGGAGTCCGTTCA+AGG	-	chr7.3:23734250-23734269	MS.gene52279:CDS	45.0%
	TTCTAGTGGTTGCAAGACCA+AGG	-	chr7.3:23734355-23734374	MS.gene52279:CDS	45.0%
!!	TTTGTTCGGTGTGCTTCTAG+TGG	-	chr7.3:23734341-23734360	MS.gene52279:CDS	45.0%
	AGCGAGAGAGTTGTCTGACA+CGG	+	chr7.3:23734163-23734182	None:intergenic	50.0%
	GCTGTTACCGAGGTCATTAC+TGG	-	chr7.3:23734180-23734199	MS.gene52279:CDS	50.0%
	TGGTTGCAAGACCAAGGCTA+TGG	-	chr7.3:23734361-23734380	MS.gene52279:CDS	50.0%
!	GCTGCTGCTGCTGTTGATAA+TGG	-	chr7.3:23734311-23734330	MS.gene52279:CDS	50.0%
	AGCGAGGGCTAAGTCTTCGT+CGG	+	chr7.3:23734093-23734112	None:intergenic	55.0%
	CAACTCTCTCGCTGTTACCG+AGG	-	chr7.3:23734170-23734189	MS.gene52279:CDS	55.0%
	GAGAGAGTTGTCTGACACGG+CGG	+	chr7.3:23734160-23734179	None:intergenic	55.0%
!	ATCGCGCCGATGACAATCGA+CGG	-	chr7.3:23734065-23734084	MS.gene52279:CDS	55.0%
!	TCGGCACCGTCGATTGTCAT+CGG	+	chr7.3:23734074-23734093	None:intergenic	55.0%
!!	GTCATCGGCGCGATTTGTTG+CGG	+	chr7.3:23734059-23734078	None:intergenic	55.0%
	AGAGTTGTCTGACACGGCGG+AGG	+	chr7.3:23734157-23734176	None:intergenic	60.0%
	GTTGCACCGCCGTGGCAAAA+AGG	-	chr7.3:23734444-23734463	MS.gene52279:CDS	60.0%
	TTATCGTGGTTGCACCGCCG+TGG	-	chr7.3:23734436-23734455	MS.gene52279:CDS	60.0%
!!	ATCGGCGCGATTTGTTGCGG+TGG	+	chr7.3:23734056-23734075	None:intergenic	60.0%
!!	GACCTCGCGGCGGGTTAAAA+CGG	+	chr7.3:23734123-23734142	None:intergenic	60.0%
	GTTGTCTGACACGGCGGAGG+CGG	+	chr7.3:23734154-23734173	None:intergenic	65.0%
!!	CTCCGTTTTAACCCGCCGCG+AGG	-	chr7.3:23734118-23734137	MS.gene52279:CDS	65.0%
!!	CTCGCGGCGGGTTAAAACGG+AGG	+	chr7.3:23734120-23734139	None:intergenic	65.0%
	GTCTGACACGGCGGAGGCGG+CGG	+	chr7.3:23734151-23734170	None:intergenic	75.0%
!	GCGGACGAGGACCTCGCGGC+GGG	+	chr7.3:23734132-23734151	None:intergenic	80.0%
!	GGCGGACGAGGACCTCGCGG+CGG	+	chr7.3:23734133-23734152	None:intergenic	80.0%
!	GGCGGCGGACGAGGACCTCG+CGG	+	chr7.3:23734136-23734155	None:intergenic	80.0%
!	CACGGCGGAGGCGGCGGACG+AGG	+	chr7.3:23734145-23734164	None:intergenic	85.0%

Chromosome	Type	Strat	End	Strand	Name
chr7.3	gene	23734032	23734469	23734032	ID=MS.gene52279
chr7.3	mRNA	23734032	23734469	23734032	ID=MS.gene52279.t1;Parent=MS.gene52279
chr7.3	exon	23734032	23734469	23734032	ID=MS.gene52279.t1.exon1;Parent=MS.gene52279.t1
chr7.3	CDS	23734032	23734469	23734032	ID=cds.MS.gene52279.t1;Parent=MS.gene52279.t1

Gene Sequence

>MS.gene52279

ATGGAAGACTCTCAACAGCAACCACCGCAACAAATCGCGCCGATGACAATCGACGGTGCCGACGAAGACTTAGCCCTCGCTAACTCCTCCGTTTTAACCCGCCGCGAGGTCCTCGTCCGCCGCCTCCGCCGTGTCAGACAACTCTCTCGCTGTTACCGAGGTCATTACTGGGCGTTAATGGAAGAGCTTAAGGCTAAGTACAGAGAGTATTACTGGACTTATGGAAGGAGTCCGTTCAAGGAAGATGAAGAAAGAAATTCGGGTAACGCTTCTGCTGCTGCTGCTGCTGCTGTTGATAATGGTGATGACTTTGTTCGGTGTGCTTCTAGTGGTTGCAAGACCAAGGCTATGGCTCTTACTAAGTATTGTCATGCGCATATTTTGTCTGATCCTAATCAGAAGCTTTATCGTGGTTGCACCGCCGTGGCAAAAAGGTGA

Protein sequence

>MS.gene52279.t1

MEDSQQQPPQQIAPMTIDGADEDLALANSSVLTRREVLVRRLRRVRQLSRCYRGHYWALMEELKAKYREYYWTYGRSPFKEDEERNSGNASAAAAAAVDNGDDFVRCASSGCKTKAMALTKYCHAHILSDPNQKLYRGCTAVAKR