Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene52559.t1 | AES97291.2 | 91.8 | 282 | 23 | 0 | 2 | 283 | 1 | 282 | 8.70E-123 | 449.9 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene52559.t1 | G7JZA6 | 91.8 | 282 | 23 | 0 | 2 | 283 | 1 | 282 | 6.3e-123 | 449.9 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene059276 | MS.gene52559 | 0.82881 | 7.31E-55 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene52559.t1 | MTR_5g048290 | 92.553 | 282 | 21 | 0 | 2 | 283 | 1 | 282 | 0.0 | 529 |
| MS.gene52559.t1 | MTR_1g054370 | 68.276 | 290 | 84 | 2 | 2 | 283 | 1 | 290 | 1.02e-131 | 375 |
| MS.gene52559.t1 | MTR_1g054360 | 63.950 | 319 | 78 | 3 | 2 | 283 | 1 | 319 | 1.64e-130 | 373 |
| MS.gene52559.t1 | MTR_1g054365 | 47.267 | 311 | 95 | 6 | 2 | 283 | 1 | 271 | 1.04e-78 | 240 |
| MS.gene52559.t1 | MTR_5g085195 | 45.865 | 266 | 79 | 5 | 20 | 283 | 53 | 255 | 1.27e-62 | 198 |
| MS.gene52559.t1 | MTR_5g085150 | 46.360 | 261 | 90 | 5 | 21 | 278 | 5 | 218 | 1.11e-58 | 187 |
| MS.gene52559.t1 | MTR_5g085190 | 59.459 | 148 | 54 | 3 | 21 | 165 | 5 | 149 | 5.51e-51 | 166 |
| MS.gene52559.t1 | MTR_1g054385 | 43.216 | 199 | 85 | 2 | 87 | 282 | 77 | 250 | 8.70e-42 | 144 |
| MS.gene52559.t1 | MTR_1g054380 | 30.151 | 199 | 89 | 3 | 78 | 266 | 31 | 189 | 8.63e-20 | 85.9 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Find 55 sgRNAs with CRISPR-Local
Find 71 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| GGGCGTGTCCGGGGAATTAT+TGG | 0.254491 | 5.2:-38657642 | None:intergenic |
| CGGAACCTGTCAGGCTAGTT+TGG | 0.281389 | 5.2:-38657928 | None:intergenic |
| GACTAAAATGCTTATTAATA+AGG | 0.297095 | 5.2:-38657856 | None:intergenic |
| GACGAAAACTCAAAGAATAA+AGG | 0.310389 | 5.2:+38658226 | MS.gene52559:CDS |
| TCAGCTCCTGTACAAATTCC+TGG | 0.347173 | 5.2:+38657710 | MS.gene52559:CDS |
| CATGTCATTAGGCAAAAGTT+AGG | 0.365477 | 5.2:+38657971 | MS.gene52559:CDS |
| TCTTCCTTCTTCATGTCATT+AGG | 0.374498 | 5.2:+38657960 | MS.gene52559:CDS |
| CTTTATCGCCTTATCATAAC+CGG | 0.377316 | 5.2:-38658347 | None:intergenic |
| GACTTTGGAAGAAACGATCT+TGG | 0.379549 | 5.2:+38658165 | MS.gene52559:CDS |
| ATGGTGATACCACATCAGTC+TGG | 0.383647 | 5.2:+38657810 | MS.gene52559:CDS |
| AGATGAATTGAAGAAGACTT+TGG | 0.411616 | 5.2:+38658150 | MS.gene52559:CDS |
| ATGTCATTAGGCAAAAGTTA+GGG | 0.413793 | 5.2:+38657972 | MS.gene52559:CDS |
| AAAGAGTTGCTTGAACATAA+TGG | 0.417254 | 5.2:+38657581 | MS.gene52559:CDS |
| GTCTGACTATGAGAAGGATA+TGG | 0.426493 | 5.2:+38658108 | MS.gene52559:CDS |
| TGAAGTAAAACGGCCTCGAC+TGG | 0.437357 | 5.2:+38657676 | MS.gene52559:CDS |
| ACGAAAACTCAAAGAATAAA+GGG | 0.445207 | 5.2:+38658227 | MS.gene52559:CDS |
| CCAGCGATGTTCTTCGGAAT+GGG | 0.465611 | 5.2:+38657739 | MS.gene52559:CDS |
| ACCAGCGATGTTCTTCGGAA+TGG | 0.473956 | 5.2:+38657738 | MS.gene52559:CDS |
| CGTTACCGTTGACAAGTTGA+AGG | 0.489635 | 5.2:+38658279 | MS.gene52559:CDS |
| TCAAAACCGTTGAAGTAAAA+CGG | 0.502228 | 5.2:+38657666 | MS.gene52559:CDS |
| TTGTACGAACGGAACCTGTC+AGG | 0.506810 | 5.2:-38657937 | None:intergenic |
| TTTGCCTAATGACATGAAGA+AGG | 0.508169 | 5.2:-38657964 | None:intergenic |
| GTACAAAGCCGGTTATGATA+AGG | 0.509385 | 5.2:+38658339 | MS.gene52559:CDS |
| ACTTGGTCAAGGTTTAAGTC+CGG | 0.520256 | 5.2:-38658389 | None:intergenic |
| AAGGAAAACAAGGCTTATGT+TGG | 0.524301 | 5.2:+38658004 | MS.gene52559:CDS |
| CACATCTTTCACAGACATCA+TGG | 0.533611 | 5.2:-38657552 | None:intergenic |
| CCCATTCCGAAGAACATCGC+TGG | 0.535366 | 5.2:-38657739 | None:intergenic |
| GTACTCCAAACTAGCCTGAC+AGG | 0.535597 | 5.2:+38657923 | MS.gene52559:CDS |
| AAGAAACGATCTTGGAAAAG+AGG | 0.539483 | 5.2:+38658173 | MS.gene52559:CDS |
| TGGTGATACCACATCAGTCT+GGG | 0.549737 | 5.2:+38657811 | MS.gene52559:CDS |
| ATGTGTAAGGAACTCTCCAC+AGG | 0.568507 | 5.2:+38657610 | MS.gene52559:CDS |
| AGCTCCAACCAATAATTCCC+CGG | 0.574878 | 5.2:+38657634 | MS.gene52559:CDS |
| TTCAGCCTTCAACTTGTCAA+CGG | 0.575722 | 5.2:-38658284 | None:intergenic |
| AAGAAGGAAGATTGTACGAA+CGG | 0.580845 | 5.2:-38657948 | None:intergenic |
| ATAATGGTCAAAGATGTGTA+AGG | 0.581384 | 5.2:+38657597 | MS.gene52559:CDS |
| TTCACTGAGATCAATAGTAG+TGG | 0.585723 | 5.2:+38657785 | MS.gene52559:CDS |
| TTATTGGTTGGAGCTTCCTG+TGG | 0.594535 | 5.2:-38657626 | None:intergenic |
| ATGTCTGTGAAAGATGTGTC+GGG | 0.595976 | 5.2:+38657557 | MS.gene52559:CDS |
| GCGATGTTCTTCGGAATGGG+TGG | 0.600929 | 5.2:+38657742 | MS.gene52559:CDS |
| GAAGTTGTCTGACTATGAGA+AGG | 0.602034 | 5.2:+38658102 | MS.gene52559:CDS |
| TTATTAATAAGGTCTCCAAA+AGG | 0.608048 | 5.2:-38657845 | None:intergenic |
| TGTCATTAGGCAAAAGTTAG+GGG | 0.612482 | 5.2:+38657973 | MS.gene52559:CDS |
| GTGTCCGGGGAATTATTGGT+TGG | 0.612929 | 5.2:-38657638 | None:intergenic |
| GAACTTGAAACCTCCAGTCG+AGG | 0.613710 | 5.2:-38657689 | None:intergenic |
| GAATATGAGAAGAATTATGT+CGG | 0.622581 | 5.2:+38658058 | MS.gene52559:CDS |
| AAAGTTAGGGGATACAGTCA+AGG | 0.627819 | 5.2:+38657985 | MS.gene52559:CDS |
| GAGATCAATAGTAGTGGTGA+TGG | 0.628104 | 5.2:+38657791 | MS.gene52559:CDS |
| CTTGGAAAAGAGGCGAATGG+TGG | 0.628630 | 5.2:+38658183 | MS.gene52559:CDS |
| AGTAAAACGGCCTCGACTGG+AGG | 0.628790 | 5.2:+38657679 | MS.gene52559:CDS |
| GATGTCTGTGAAAGATGTGT+CGG | 0.637818 | 5.2:+38657556 | MS.gene52559:CDS |
| GGATACAGTCAAGGAAAACA+AGG | 0.641640 | 5.2:+38657994 | MS.gene52559:CDS |
| ATTATCACCCCAGACTGATG+TGG | 0.645625 | 5.2:-38657819 | None:intergenic |
| CGGAATGGGTGGATGAAATG+TGG | 0.646080 | 5.2:+38657753 | MS.gene52559:CDS |
| GATCTTGGAAAAGAGGCGAA+TGG | 0.677210 | 5.2:+38658180 | MS.gene52559:CDS |
| GGTGATACCACATCAGTCTG+GGG | 0.756996 | 5.2:+38657812 | MS.gene52559:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | AAAGTTTCATGAAAAAGTTA+AGG | + | chr5.2:38657883-38657902 | MS.gene52559:CDS | 20.0% |
| !! | GACTAAAATGCTTATTAATA+AGG | - | chr5.2:38657859-38657878 | None:intergenic | 20.0% |
| !!! | TATTAATAAGCATTTTAGTC+AGG | + | chr5.2:38657859-38657878 | MS.gene52559:CDS | 20.0% |
| ! | ACGAAAACTCAAAGAATAAA+GGG | + | chr5.2:38658227-38658246 | MS.gene52559:CDS | 25.0% |
| ! | GAATATGAGAAGAATTATGT+CGG | + | chr5.2:38658058-38658077 | MS.gene52559:CDS | 25.0% |
| ! | TTATTAATAAGGTCTCCAAA+AGG | - | chr5.2:38657848-38657867 | None:intergenic | 25.0% |
| AAAGAGTTGCTTGAACATAA+TGG | + | chr5.2:38657581-38657600 | MS.gene52559:CDS | 30.0% | |
| AGATGAATTGAAGAAGACTT+TGG | + | chr5.2:38658150-38658169 | MS.gene52559:CDS | 30.0% | |
| ATAATGGTCAAAGATGTGTA+AGG | + | chr5.2:38657597-38657616 | MS.gene52559:CDS | 30.0% | |
| ATGTCATTAGGCAAAAGTTA+GGG | + | chr5.2:38657972-38657991 | MS.gene52559:CDS | 30.0% | |
| GACGAAAACTCAAAGAATAA+AGG | + | chr5.2:38658226-38658245 | MS.gene52559:CDS | 30.0% | |
| TCAAAACCGTTGAAGTAAAA+CGG | + | chr5.2:38657666-38657685 | MS.gene52559:CDS | 30.0% | |
| TCATGAAAAAGTTAAGGAGT+TGG | + | chr5.2:38657889-38657908 | MS.gene52559:CDS | 30.0% | |
| !! | TTTTACTTCAACGGTTTTGA+GGG | - | chr5.2:38657666-38657685 | None:intergenic | 30.0% |
| !!! | TTTTTTCCAGGAATTTGTAC+AGG | - | chr5.2:38657719-38657738 | None:intergenic | 30.0% |
| AAAAAACCAGCGATGTTCTT+CGG | + | chr5.2:38657733-38657752 | MS.gene52559:CDS | 35.0% | |
| AAGAAACGATCTTGGAAAAG+AGG | + | chr5.2:38658173-38658192 | MS.gene52559:CDS | 35.0% | |
| AAGAAGGAAGATTGTACGAA+CGG | - | chr5.2:38657951-38657970 | None:intergenic | 35.0% | |
| AAGGAAAACAAGGCTTATGT+TGG | + | chr5.2:38658004-38658023 | MS.gene52559:CDS | 35.0% | |
| CATGTCATTAGGCAAAAGTT+AGG | + | chr5.2:38657971-38657990 | MS.gene52559:CDS | 35.0% | |
| CTTTATCGCCTTATCATAAC+CGG | - | chr5.2:38658350-38658369 | None:intergenic | 35.0% | |
| TCTTCCTTCTTCATGTCATT+AGG | + | chr5.2:38657960-38657979 | MS.gene52559:CDS | 35.0% | |
| TGTCATTAGGCAAAAGTTAG+GGG | + | chr5.2:38657973-38657992 | MS.gene52559:CDS | 35.0% | |
| TTCACTGAGATCAATAGTAG+TGG | + | chr5.2:38657785-38657804 | MS.gene52559:CDS | 35.0% | |
| TTTGCCTAATGACATGAAGA+AGG | - | chr5.2:38657967-38657986 | None:intergenic | 35.0% | |
| ! | GTTTTACTTCAACGGTTTTG+AGG | - | chr5.2:38657667-38657686 | None:intergenic | 35.0% |
| ! | TGGGGTGATAATTTTCCTTT+TGG | + | chr5.2:38657830-38657849 | MS.gene52559:CDS | 35.0% |
| !! | ATTTTAGTGCAGTACAAAGC+CGG | + | chr5.2:38658328-38658347 | MS.gene52559:CDS | 35.0% |
| !! | TCTTTGAGTTTTCGTCCATT+AGG | - | chr5.2:38658222-38658241 | None:intergenic | 35.0% |
| !!! | CAAGTTGTATTTTTTGCGTC+CGG | + | chr5.2:38658370-38658389 | MS.gene52559:CDS | 35.0% |
| !!! | TTTACTTCAACGGTTTTGAG+GGG | - | chr5.2:38657665-38657684 | None:intergenic | 35.0% |
| AAAGTTAGGGGATACAGTCA+AGG | + | chr5.2:38657985-38658004 | MS.gene52559:CDS | 40.0% | |
| ATGTCTGTGAAAGATGTGTC+GGG | + | chr5.2:38657557-38657576 | MS.gene52559:CDS | 40.0% | |
| GAAGTTGTCTGACTATGAGA+AGG | + | chr5.2:38658102-38658121 | MS.gene52559:CDS | 40.0% | |
| GAGATCAATAGTAGTGGTGA+TGG | + | chr5.2:38657791-38657810 | MS.gene52559:CDS | 40.0% | |
| GATGTCTGTGAAAGATGTGT+CGG | + | chr5.2:38657556-38657575 | MS.gene52559:CDS | 40.0% | |
| GGATACAGTCAAGGAAAACA+AGG | + | chr5.2:38657994-38658013 | MS.gene52559:CDS | 40.0% | |
| GGCAAGAGAAAAAGACCTAA+TGG | + | chr5.2:38658204-38658223 | MS.gene52559:CDS | 40.0% | |
| GTACAAAGCCGGTTATGATA+AGG | + | chr5.2:38658339-38658358 | MS.gene52559:CDS | 40.0% | |
| GTCTGACTATGAGAAGGATA+TGG | + | chr5.2:38658108-38658127 | MS.gene52559:CDS | 40.0% | |
| TTCAGCCTTCAACTTGTCAA+CGG | - | chr5.2:38658287-38658306 | None:intergenic | 40.0% | |
| ! | GACTTTGGAAGAAACGATCT+TGG | + | chr5.2:38658165-38658184 | MS.gene52559:CDS | 40.0% |
| AGCTCCAACCAATAATTCCC+CGG | + | chr5.2:38657634-38657653 | MS.gene52559:CDS | 45.0% | |
| ATGGTGATACCACATCAGTC+TGG | + | chr5.2:38657810-38657829 | MS.gene52559:CDS | 45.0% | |
| ATGTGTAAGGAACTCTCCAC+AGG | + | chr5.2:38657610-38657629 | MS.gene52559:CDS | 45.0% | |
| ATTATCACCCCAGACTGATG+TGG | - | chr5.2:38657822-38657841 | None:intergenic | 45.0% | |
| GATCTTGGAAAAGAGGCGAA+TGG | + | chr5.2:38658180-38658199 | MS.gene52559:CDS | 45.0% | |
| TCAGCTCCTGTACAAATTCC+TGG | + | chr5.2:38657710-38657729 | MS.gene52559:CDS | 45.0% | |
| TGGTGATACCACATCAGTCT+GGG | + | chr5.2:38657811-38657830 | MS.gene52559:CDS | 45.0% | |
| ! | CGTTACCGTTGACAAGTTGA+AGG | + | chr5.2:38658279-38658298 | MS.gene52559:CDS | 45.0% |
| ! | TTATTGGTTGGAGCTTCCTG+TGG | - | chr5.2:38657629-38657648 | None:intergenic | 45.0% |
| !! | TCGAGGCCGTTTTACTTCAA+CGG | - | chr5.2:38657675-38657694 | None:intergenic | 45.0% |
| !!! | GAACATCGCTGGTTTTTTCC+AGG | - | chr5.2:38657731-38657750 | None:intergenic | 45.0% |
| ACCAGCGATGTTCTTCGGAA+TGG | + | chr5.2:38657738-38657757 | MS.gene52559:CDS | 50.0% | |
| CCAGCGATGTTCTTCGGAAT+GGG | + | chr5.2:38657739-38657758 | MS.gene52559:CDS | 50.0% | |
| CGGAATGGGTGGATGAAATG+TGG | + | chr5.2:38657753-38657772 | MS.gene52559:CDS | 50.0% | |
| CTTGGAAAAGAGGCGAATGG+TGG | + | chr5.2:38658183-38658202 | MS.gene52559:CDS | 50.0% | |
| GAACTTGAAACCTCCAGTCG+AGG | - | chr5.2:38657692-38657711 | None:intergenic | 50.0% | |
| GGTGATACCACATCAGTCTG+GGG | + | chr5.2:38657812-38657831 | MS.gene52559:CDS | 50.0% | |
| GTGTCCGGGGAATTATTGGT+TGG | - | chr5.2:38657641-38657660 | None:intergenic | 50.0% | |
| TGAAGTAAAACGGCCTCGAC+TGG | + | chr5.2:38657676-38657695 | MS.gene52559:CDS | 50.0% | |
| TTGTACGAACGGAACCTGTC+AGG | - | chr5.2:38657940-38657959 | None:intergenic | 50.0% | |
| ! | GTACTCCAAACTAGCCTGAC+AGG | + | chr5.2:38657923-38657942 | MS.gene52559:CDS | 50.0% |
| AGTAAAACGGCCTCGACTGG+AGG | + | chr5.2:38657679-38657698 | MS.gene52559:CDS | 55.0% | |
| CCCATTCCGAAGAACATCGC+TGG | - | chr5.2:38657742-38657761 | None:intergenic | 55.0% | |
| GCGATGTTCTTCGGAATGGG+TGG | + | chr5.2:38657742-38657761 | MS.gene52559:CDS | 55.0% | |
| ! | CGGAACCTGTCAGGCTAGTT+TGG | - | chr5.2:38657931-38657950 | None:intergenic | 55.0% |
| GGGCGTGTCCGGGGAATTAT+TGG | - | chr5.2:38657645-38657664 | None:intergenic | 60.0% | |
| !!! | ACGGTTTTGAGGGGCGTGTC+CGG | - | chr5.2:38657656-38657675 | None:intergenic | 60.0% |
| !!! | CGGTTTTGAGGGGCGTGTCC+GGG | - | chr5.2:38657655-38657674 | None:intergenic | 65.0% |
| !!! | GGTTTTGAGGGGCGTGTCCG+GGG | - | chr5.2:38657654-38657673 | None:intergenic | 65.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr5.2 | gene | 38657554 | 38658405 | 38657554 | ID=MS.gene52559 |
| chr5.2 | mRNA | 38657554 | 38658405 | 38657554 | ID=MS.gene52559.t1;Parent=MS.gene52559 |
| chr5.2 | exon | 38657554 | 38658405 | 38657554 | ID=MS.gene52559.t1.exon1;Parent=MS.gene52559.t1 |
| chr5.2 | CDS | 38657554 | 38658405 | 38657554 | ID=cds.MS.gene52559.t1;Parent=MS.gene52559.t1 |
| Gene Sequence |
| Protein sequence |
