AlfalfaGEDB | MS.gene059276

M. sativa cultivar XinJiangDaYe / MS.gene059276

KEGG: -
GO: GO:0003674 GO:0003676 GO:0005488 GO:0008270 GO:0043167 GO:0043169 GO:0046872 GO:0046914 GO:0097159 GO:1901363
PFAM: -

Nr:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene059276.t1	XP_028762943.1	64.9	94	27	1	12	99	255	348	3.10E-27	131

Swissprot:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score

Trembl:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene059276.t1	A0A151TBS9	63.6	88	31	1	10	97	136	222	3.6e-25	123.6

TFs/TRs:

Gene ID	Type	Classification

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network:

Gene1	Gene2	correlation coefficient	p_value	FDR
MS.gene049262	MS.gene059276	0.823432	1.39E-53	-1.69E-46
MS.gene049701	MS.gene059276	0.816276	6.01E-52	-1.69E-46
MS.gene049853	MS.gene059276	0.800525	1.38E-48	-1.69E-46
MS.gene050026	MS.gene059276	0.803925	2.76E-49	-1.69E-46
MS.gene050221	MS.gene059276	0.831115	2.00E-55	-1.69E-46
MS.gene051710	MS.gene059276	0.829759	4.30E-55	-1.69E-46
MS.gene05422	MS.gene059276	0.811222	7.80E-51	-1.69E-46
MS.gene05423	MS.gene059276	0.802226	6.20E-49	-1.69E-46
MS.gene054651	MS.gene059276	0.801957	7.05E-49	-1.69E-46
MS.gene054801	MS.gene059276	0.809804	1.58E-50	-1.69E-46
MS.gene054991	MS.gene059276	0.807648	4.56E-50	-1.69E-46
MS.gene055056	MS.gene059276	0.821132	4.75E-53	-1.69E-46
MS.gene055058	MS.gene059276	0.824278	8.80E-54	-1.69E-46
MS.gene056854	MS.gene059276	0.800455	1.43E-48	-1.69E-46
MS.gene05762	MS.gene059276	0.808439	3.10E-50	-1.69E-46
MS.gene058515	MS.gene059276	0.829362	5.37E-55	-1.69E-46
MS.gene059272	MS.gene059276	0.822935	1.81E-53	-1.69E-46
MS.gene059276	MS.gene059584	0.801697	7.97E-49	-1.69E-46
MS.gene059276	MS.gene059601	0.819087	1.40E-52	-1.69E-46
MS.gene059276	MS.gene061485	0.807888	4.06E-50	-1.69E-46
MS.gene059276	MS.gene06269	0.826537	2.57E-54	-1.69E-46
MS.gene059276	MS.gene063357	0.811694	6.16E-51	-1.69E-46
MS.gene059276	MS.gene06365	0.807319	5.36E-50	-1.69E-46
MS.gene059276	MS.gene063846	0.824606	7.37E-54	-1.69E-46
MS.gene059276	MS.gene064007	0.80181	7.55E-49	-1.69E-46
MS.gene059276	MS.gene064075	0.841132	5.72E-58	-1.69E-46
MS.gene059276	MS.gene064192	0.804443	2.15E-49	-1.69E-46
MS.gene059276	MS.gene064600	0.820375	7.09E-53	-1.69E-46
MS.gene059276	MS.gene065209	0.838374	2.99E-57	-1.69E-46
MS.gene059276	MS.gene066313	0.824672	7.11E-54	-1.69E-46
MS.gene059276	MS.gene066335	0.819989	8.69E-53	-1.69E-46
MS.gene059276	MS.gene066752	0.832058	1.17E-55	-1.69E-46
MS.gene059276	MS.gene067656	0.815337	9.73E-52	-1.69E-46
MS.gene059276	MS.gene068012	0.80543	1.34E-49	-1.69E-46
MS.gene059276	MS.gene068308	0.829603	4.69E-55	-1.69E-46
MS.gene059276	MS.gene068403	0.813028	3.15E-51	-1.69E-46
MS.gene059276	MS.gene068452	0.810029	1.41E-50	-1.69E-46
MS.gene059276	MS.gene068453	0.824541	7.63E-54	-1.69E-46
MS.gene059276	MS.gene068494	0.811963	5.38E-51	-1.69E-46
MS.gene059276	MS.gene068924	0.800832	1.20E-48	-1.69E-46
MS.gene059276	MS.gene070301	0.804832	1.79E-49	-1.69E-46
MS.gene059276	MS.gene070444	0.82575	3.95E-54	-1.69E-46
MS.gene059276	MS.gene070466	0.843366	1.46E-58	-1.69E-46
MS.gene059276	MS.gene070467	0.853204	2.79E-61	-1.69E-46
MS.gene059276	MS.gene071330	0.838975	2.09E-57	-1.69E-46
MS.gene059276	MS.gene072067	0.804009	2.65E-49	-1.69E-46
MS.gene059276	MS.gene072819	0.803519	3.35E-49	-1.69E-46
MS.gene059276	MS.gene072915	0.828362	9.38E-55	-1.69E-46
MS.gene059276	MS.gene09348	0.807475	4.96E-50	-1.69E-46
MS.gene059276	MS.gene09451	0.822327	2.51E-53	-1.69E-46
MS.gene059276	MS.gene09504	0.85497	8.63E-62	-1.69E-46
MS.gene059276	MS.gene20657	0.86306	3.26E-64	-1.69E-46
MS.gene059276	MS.gene22611	0.837851	4.07E-57	-1.69E-46
MS.gene059276	MS.gene23940	0.81407	1.86E-51	-1.69E-46
MS.gene059276	MS.gene24211	0.830379	3.04E-55	-1.69E-46
MS.gene059276	MS.gene25854	0.832556	8.84E-56	-1.69E-46
MS.gene059276	MS.gene26056	0.865518	5.57E-65	-1.69E-46
MS.gene059276	MS.gene26098	0.803083	4.13E-49	-1.69E-46
MS.gene059276	MS.gene27421	0.804096	2.55E-49	-1.69E-46
MS.gene059276	MS.gene27720	0.801956	7.05E-49	-1.69E-46
MS.gene059276	MS.gene28203	0.82121	4.56E-53	-1.69E-46
MS.gene059276	MS.gene28461	0.809481	1.85E-50	-1.69E-46
MS.gene059276	MS.gene28508	0.80367	3.12E-49	-1.69E-46
MS.gene059276	MS.gene29008	0.801533	8.61E-49	-1.69E-46
MS.gene059276	MS.gene29009	0.837546	4.88E-57	-1.69E-46
MS.gene059276	MS.gene29850	0.820198	7.78E-53	-1.69E-46
MS.gene059276	MS.gene30073	0.840506	8.34E-58	-1.69E-46
MS.gene059276	MS.gene30139	0.837332	5.53E-57	-1.69E-46
MS.gene059276	MS.gene30580	0.870704	1.19E-66	-1.69E-46
MS.gene059276	MS.gene31231	0.814122	1.81E-51	-1.69E-46
MS.gene059276	MS.gene32747	0.812644	3.82E-51	-1.69E-46
MS.gene059276	MS.gene33228	0.844953	5.49E-59	-1.69E-46
MS.gene059276	MS.gene33462	0.823384	1.43E-53	-1.69E-46
MS.gene059276	MS.gene34251	0.828589	8.27E-55	-1.69E-46
MS.gene059276	MS.gene34615	0.855903	4.62E-62	-1.69E-46
MS.gene059276	MS.gene38011	0.836648	8.27E-57	-1.69E-46
MS.gene059276	MS.gene38187	0.805767	1.14E-49	-1.69E-46
MS.gene059276	MS.gene38429	0.850456	1.68E-60	-1.69E-46
MS.gene059276	MS.gene38444	0.8271	1.88E-54	-1.69E-46
MS.gene059276	MS.gene38850	0.853455	2.36E-61	-1.69E-46
MS.gene059276	MS.gene40925	0.828294	9.74E-55	-1.69E-46
MS.gene059276	MS.gene41240	0.80618	9.32E-50	-1.69E-46
MS.gene059276	MS.gene41450	0.80145	8.95E-49	-1.69E-46
MS.gene059276	MS.gene41507	0.820824	5.59E-53	-1.69E-46
MS.gene059276	MS.gene41508	0.803387	3.57E-49	-1.69E-46
MS.gene059276	MS.gene42887	0.839495	1.53E-57	-1.69E-46
MS.gene059276	MS.gene42891	0.839473	1.55E-57	-1.69E-46
MS.gene059276	MS.gene43560	0.803194	3.92E-49	-1.69E-46
MS.gene059276	MS.gene44575	0.802754	4.83E-49	-1.69E-46
MS.gene059276	MS.gene44743	0.823324	1.47E-53	-1.69E-46
MS.gene059276	MS.gene45794	0.844606	6.81E-59	-1.69E-46
MS.gene059276	MS.gene46082	0.805126	1.55E-49	-1.69E-46
MS.gene059276	MS.gene46359	0.840795	7.01E-58	-1.69E-46
MS.gene059276	MS.gene47167	0.816214	6.21E-52	-1.69E-46
MS.gene059276	MS.gene47552	0.826103	3.26E-54	-1.69E-46
MS.gene059276	MS.gene47943	0.805165	1.52E-49	-1.69E-46
MS.gene059276	MS.gene48858	0.952359	2.87E-110	-1.69E-46
MS.gene059276	MS.gene48919	0.840365	9.08E-58	-1.69E-46
MS.gene059276	MS.gene51189	0.838113	3.49E-57	-1.69E-46
MS.gene059276	MS.gene51252	0.83579	1.37E-56	-1.69E-46
MS.gene059276	MS.gene52525	0.833298	5.78E-56	-1.69E-46
MS.gene059276	MS.gene52559	0.82881	7.31E-55	-1.69E-46
MS.gene059276	MS.gene53827	0.804621	1.98E-49	-1.69E-46
MS.gene059276	MS.gene54320	0.821393	4.14E-53	-1.69E-46
MS.gene059276	MS.gene54717	0.809608	1.74E-50	-1.69E-46
MS.gene059276	MS.gene54921	0.853485	2.32E-61	-1.69E-46
MS.gene059276	MS.gene56618	0.832458	9.35E-56	-1.69E-46
MS.gene059276	MS.gene57103	0.814167	1.77E-51	-1.69E-46
MS.gene059276	MS.gene57808	0.813552	2.42E-51	-1.69E-46
MS.gene059276	MS.gene58638	0.835783	1.37E-56	-1.69E-46
MS.gene059276	MS.gene58802	0.801668	8.08E-49	-1.69E-46
MS.gene059276	MS.gene59780	0.843863	1.08E-58	-1.69E-46
MS.gene059276	MS.gene61742	0.881164	3.01E-70	-1.69E-46
MS.gene059276	MS.gene62748	0.85138	9.21E-61	-1.69E-46
MS.gene059276	MS.gene63141	0.808468	3.05E-50	-1.69E-46
MS.gene059276	MS.gene63142	0.806599	7.61E-50	-1.69E-46
MS.gene059276	MS.gene63607	0.803062	4.17E-49	-1.69E-46
MS.gene059276	MS.gene64383	0.824299	8.70E-54	-1.69E-46
MS.gene059276	MS.gene64567	0.842226	2.94E-58	-1.69E-46
MS.gene059276	MS.gene65003	0.828305	9.68E-55	-1.69E-46
MS.gene059276	MS.gene66192	0.853142	2.91E-61	-1.69E-46
MS.gene059276	MS.gene66682	0.855246	7.17E-62	-1.69E-46
MS.gene059276	MS.gene66686	0.818952	1.50E-52	-1.69E-46
MS.gene059276	MS.gene66688	0.808914	2.45E-50	-1.69E-46
MS.gene059276	MS.gene66936	0.811673	6.22E-51	-1.69E-46
MS.gene059276	MS.gene67182	0.82884	7.19E-55	-1.69E-46
MS.gene059276	MS.gene67618	0.814508	1.49E-51	-1.69E-46
MS.gene059276	MS.gene67991	0.811739	6.02E-51	-1.69E-46
MS.gene059276	MS.gene68999	0.83462	2.70E-56	-1.69E-46
MS.gene059276	MS.gene69102	0.830622	2.65E-55	-1.69E-46
MS.gene059276	MS.gene69594	0.822657	2.11E-53	-1.69E-46
MS.gene059276	MS.gene70908	0.844902	5.67E-59	-1.69E-46
MS.gene059276	MS.gene71430	0.808716	2.70E-50	-1.69E-46
MS.gene059276	MS.gene72205	0.817641	2.97E-52	-1.69E-46
MS.gene059276	MS.gene73540	0.808684	2.74E-50	-1.69E-46
MS.gene059276	MS.gene74025	0.822826	1.92E-53	-1.69E-46
MS.gene059276	MS.gene76275	0.814973	1.17E-51	-1.69E-46
MS.gene059276	MS.gene76389	0.837291	5.67E-57	-1.69E-46
MS.gene059276	MS.gene77862	0.853189	2.82E-61	-1.69E-46
MS.gene059276	MS.gene77863	0.843218	1.60E-58	-1.69E-46
MS.gene059276	MS.gene78647	0.836927	7.02E-57	-1.69E-46
MS.gene059276	MS.gene79147	0.833649	4.72E-56	-1.69E-46
MS.gene059276	MS.gene79723	0.801624	8.25E-49	-1.69E-46
MS.gene059276	MS.gene79824	0.813574	2.39E-51	-1.69E-46
MS.gene059276	MS.gene80427	0.802534	5.36E-49	-1.69E-46
MS.gene059276	MS.gene80844	0.808845	2.54E-50	-1.69E-46
MS.gene059276	MS.gene82614	0.815481	9.05E-52	-1.69E-46
MS.gene059276	MS.gene82663	0.811031	8.58E-51	-1.69E-46
MS.gene059276	MS.gene82668	0.847203	1.34E-59	-1.69E-46
MS.gene059276	MS.gene82669	0.819881	9.20E-53	-1.69E-46
MS.gene059276	MS.gene82682	0.844598	6.85E-59	-1.69E-46
MS.gene059276	MS.gene82793	0.803744	3.01E-49	-1.69E-46
MS.gene059276	MS.gene82962	0.863065	3.25E-64	-1.69E-46
MS.gene059276	MS.gene83166	0.800922	1.15E-48	-1.69E-46
MS.gene059276	MS.gene84001	0.812795	3.54E-51	-1.69E-46
MS.gene059276	MS.gene84968	0.847574	1.06E-59	-1.69E-46
MS.gene059276	MS.gene84994	0.829366	5.36E-55	-1.69E-46
MS.gene059276	MS.gene85079	0.807954	3.93E-50	-1.69E-46
MS.gene059276	MS.gene85361	0.819873	9.24E-53	-1.69E-46
MS.gene059276	MS.gene85436	0.826371	2.81E-54	-1.69E-46
MS.gene059276	MS.gene85694	0.838929	2.15E-57	-1.69E-46
MS.gene059276	MS.gene85698	0.849872	2.45E-60	-1.69E-46
MS.gene059276	MS.gene87216	0.820429	6.89E-53	-1.69E-46
MS.gene059276	MS.gene88533	0.833581	4.91E-56	-1.69E-46
MS.gene059276	MS.gene88539	0.844372	7.87E-59	-1.69E-46
MS.gene059276	MS.gene88548	0.847607	1.04E-59	-1.69E-46
MS.gene059276	MS.gene89778	0.811248	7.70E-51	-1.69E-46
MS.gene059276	MS.gene91451	0.839437	1.59E-57	-1.69E-46
MS.gene059276	MS.gene92276	0.808808	2.58E-50	-1.69E-46
MS.gene059276	MS.gene94001	0.812068	5.11E-51	-1.69E-46
MS.gene059276	MS.gene96078	0.804114	2.52E-49	-1.69E-46
MS.gene059276	MS.gene97534	0.835819	1.34E-56	-1.69E-46
MS.gene059276	MS.gene97797	0.835396	1.72E-56	-1.69E-46
MS.gene059276	MS.gene98550	0.869782	2.39E-66	-1.69E-46
MS.gene059276	MS.gene98673	0.837804	4.19E-57	-1.69E-46
MS.gene059276	MS.gene98774	0.816808	4.57E-52	-1.69E-46

PPI:

Gene1	Gene2	Type

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene059276.t1	MTR_1g036690	58.537	82	27	3	19	99	5	80	4.34e-24	87.4

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score

Find 14 sgRNAs with CRISPR-Local

Find 17 sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
GATTTCTTTGTTGATCCTTA+TGG	0.227069	4.4:-74743230	MS.gene059276:CDS
CACTTCAAAATAAGGATAAA+AGG	0.234592	4.4:-74743328	MS.gene059276:CDS
CCAACAATCACTTCAAAATA+AGG	0.244294	4.4:-74743336	MS.gene059276:CDS
AGAAATCAGCAGCATCTAAA+TGG	0.308817	4.4:+74743246	None:intergenic
TAAAGATCAACAAGATGTTT+TGG	0.333763	4.4:+74743359	None:intergenic
TGAAGATGATTATGGTAATA+TGG	0.338642	4.4:-74743276	MS.gene059276:CDS
GAAAATGATGAAGATGATTA+TGG	0.394855	4.4:-74743284	MS.gene059276:CDS
GGAATGATTGATCATCTCAT+TGG	0.401226	4.4:-74743209	MS.gene059276:CDS
GTTGTGGAGGCAAAGGTCAT+TGG	0.462627	4.4:-74743400	MS.gene059276:CDS
ACTTCAAAATAAGGATAAAA+GGG	0.487291	4.4:-74743327	MS.gene059276:CDS
TGCTATCGTTGTGGAGGCAA+AGG	0.489090	4.4:-74743407	MS.gene059276:CDS
AGATGATCAATCATTCCATA+AGG	0.536978	4.4:+74743215	None:intergenic
GAGAGTATTTGCTATCGTTG+TGG	0.583351	4.4:-74743416	MS.gene059276:CDS
AGTATTTGCTATCGTTGTGG+AGG	0.613176	4.4:-74743413	MS.gene059276:CDS

CRISPR-GE

badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content
!!	AATGTTGAAAAAAATGATAT+TGG	-	chr4.4:74743193-74743212	MS.gene059276:CDS	15.0%
!!	ACTTCAAAATAAGGATAAAA+GGG	-	chr4.4:74743324-74743343	MS.gene059276:CDS	20.0%
!	CACTTCAAAATAAGGATAAA+AGG	-	chr4.4:74743323-74743342	MS.gene059276:CDS	25.0%
!	GAAAATGATGAAGATGATTA+TGG	-	chr4.4:74743367-74743386	MS.gene059276:CDS	25.0%
!	TGAAGATGATTATGGTAATA+TGG	-	chr4.4:74743375-74743394	MS.gene059276:CDS	25.0%
!!	TAAAGATCAACAAGATGTTT+TGG	+	chr4.4:74743295-74743314	None:intergenic	25.0%
	AGATGATCAATCATTCCATA+AGG	+	chr4.4:74743439-74743458	None:intergenic	30.0%
	CCAACAATCACTTCAAAATA+AGG	-	chr4.4:74743315-74743334	MS.gene059276:CDS	30.0%
	GATTTCTTTGTTGATCCTTA+TGG	-	chr4.4:74743421-74743440	MS.gene059276:CDS	30.0%
!!!	CCTTATTTTGAAGTGATTGT+TGG	+	chr4.4:74743318-74743337	None:intergenic	30.0%
	AGAAATCAGCAGCATCTAAA+TGG	+	chr4.4:74743408-74743427	None:intergenic	35.0%
	GGAATGATTGATCATCTCAT+TGG	-	chr4.4:74743442-74743461	MS.gene059276:CDS	35.0%
!!	AGTATTTGCTATCGTTGTGG+AGG	-	chr4.4:74743238-74743257	MS.gene059276:CDS	40.0%
!!	GAGAGTATTTGCTATCGTTG+TGG	-	chr4.4:74743235-74743254	MS.gene059276:CDS	40.0%
!!!	AAGATGTTTTGGCGTACGAC+AGG	+	chr4.4:74743284-74743303	None:intergenic	45.0%
	GTTGTGGAGGCAAAGGTCAT+TGG	-	chr4.4:74743251-74743270	MS.gene059276:CDS	50.0%
	TGCTATCGTTGTGGAGGCAA+AGG	-	chr4.4:74743244-74743263	MS.gene059276:CDS	50.0%

Chromosome	Type	Strat	End	Strand	Name
chr4.4	gene	74743187	74743486	74743187	ID=MS.gene059276
chr4.4	mRNA	74743187	74743486	74743187	ID=MS.gene059276.t1;Parent=MS.gene059276
chr4.4	exon	74743187	74743486	74743187	ID=MS.gene059276.t1.exon1;Parent=MS.gene059276.t1
chr4.4	CDS	74743187	74743486	74743187	ID=cds.MS.gene059276.t1;Parent=MS.gene059276.t1

Gene Sequence	>MS.gene059276 ATGAAAAATGTTGAAAAAAATGATATTGGTCAAAGCAGCAAAAGTAATGAGAGTATTTGCTATCGTTGTGGAGGCAAAGGTCATTGGAGTCGTACCTGTCGTACGCCAAAACATCTTGTTGATCTTTACCAACAATCACTTCAAAATAAGGATAAAAGGGCTGAAACGCATTTTGCATGTGAAAATGATGAAGATGATTATGGTAATATGGAAAGTATCCATTTAGATGCTGCTGATTTCTTTGTTGATCCTTATGGAATGATTGATCATCTCATTGGAGATGAAAATGTCCAGAAATAA
Protein sequence	>MS.gene059276.t1 MKNVEKNDIGQSSKSNESICYRCGGKGHWSRTCRTPKHLVDLYQQSLQNKDKRAETHFACENDEDDYGNMESIHLDAADFFVDPYGMIDHLIGDENVQK