AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene52943

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene52943.t1 MTR_1g093120 92.818 181 13 0 1 181 1 181 1.55e-121 351
MS.gene52943.t1 MTR_1g093120 92.222 90 7 0 171 260 281 370 6.24e-50 168
MS.gene52943.t1 MTR_1g093110 80.110 181 34 1 1 179 2 182 4.97e-102 301
MS.gene52943.t1 MTR_1g093110 74.725 91 22 1 171 260 284 374 7.33e-38 136
MS.gene52943.t1 MTR_1g093140 77.717 184 35 3 1 178 1 184 1.66e-100 298
MS.gene52943.t1 MTR_1g093140 83.696 92 13 1 171 260 287 378 5.29e-45 155
MS.gene52943.t1 MTR_7g078010 60.109 183 63 4 1 178 1 178 2.63e-70 218
MS.gene52943.t1 MTR_7g078010 60.109 183 63 4 1 178 1 178 3.30e-69 218
MS.gene52943.t1 MTR_7g078010 56.989 93 37 1 171 260 281 373 2.10e-27 108
MS.gene52943.t1 MTR_4g119410 59.649 171 65 2 8 178 12 178 2.50e-67 213
MS.gene52943.t1 MTR_4g119410 67.742 93 27 1 171 260 281 373 8.94e-36 131
MS.gene52943.t1 MTR_7g056347 58.857 175 68 2 4 178 8 178 2.90e-67 213
MS.gene52943.t1 MTR_7g056347 58.065 93 36 1 171 260 281 373 1.71e-29 114
MS.gene52943.t1 MTR_4g024660 57.143 182 70 3 1 178 1 178 2.98e-66 210
MS.gene52943.t1 MTR_4g024660 54.545 110 43 2 158 260 264 373 1.08e-30 117
MS.gene52943.t1 MTR_8g062600 56.044 182 72 3 1 178 1 178 7.45e-66 209
MS.gene52943.t1 MTR_8g062600 57.273 110 40 2 158 260 264 373 7.63e-32 120
MS.gene52943.t1 MTR_8g006930 58.235 170 67 2 9 178 13 178 3.09e-65 207
MS.gene52943.t1 MTR_8g006930 63.441 93 31 1 171 260 281 373 2.30e-33 124
MS.gene52943.t1 MTR_8g063070 53.552 183 77 3 1 179 1 179 3.92e-64 205
MS.gene52943.t1 MTR_8g063070 56.989 93 37 1 171 260 281 373 1.58e-26 106
MS.gene52943.t1 MTR_7g056393 54.945 182 76 3 2 183 8 183 3.34e-63 202
MS.gene52943.t1 MTR_7g056393 66.667 93 28 1 171 260 281 373 6.89e-36 131
MS.gene52943.t1 MTR_4g099380 58.182 165 62 2 15 179 124 281 1.79e-62 197
MS.gene52943.t1 MTR_2g069010 51.724 174 82 1 8 181 12 183 2.61e-61 197
MS.gene52943.t1 MTR_2g069010 59.574 94 33 2 171 260 283 375 6.03e-27 107
MS.gene52943.t1 MTR_2g069020 51.724 174 82 1 8 181 10 181 4.12e-61 197
MS.gene52943.t1 MTR_2g069020 60.440 91 34 2 171 260 281 370 9.83e-25 101
MS.gene52943.t1 MTR_2g069020 51.724 174 82 1 8 181 10 181 1.20e-60 196
MS.gene52943.t1 MTR_2g069020 60.440 91 34 2 171 260 281 370 1.25e-24 101
MS.gene52943.t1 MTR_2g068950 50.296 169 82 1 13 181 11 177 1.61e-56 185
MS.gene52943.t1 MTR_2g068950 54.255 94 38 2 171 260 277 369 1.60e-23 98.2
MS.gene52943.t1 MTR_2g068960 50.296 169 82 1 13 181 11 177 2.22e-56 185
MS.gene52943.t1 MTR_2g068960 53.191 94 39 2 171 260 277 369 7.01e-23 96.3
MS.gene52943.t1 MTR_8g469400 53.005 183 66 4 1 179 1 167 1.47e-55 182
MS.gene52943.t1 MTR_8g469400 62.366 93 32 1 171 260 268 360 8.93e-33 123
MS.gene52943.t1 MTR_7g056370 46.995 183 82 4 25 197 8 185 1.59e-51 167
MS.gene52943.t1 MTR_2g069060 42.791 215 89 4 4 218 8 188 2.26e-50 169
MS.gene52943.t1 MTR_2g069060 57.778 90 35 2 171 260 263 349 1.05e-24 101
MS.gene52943.t1 MTR_2g069070 46.591 176 72 2 4 179 8 161 1.57e-49 161
MS.gene52943.t1 MTR_8g009080 46.961 181 93 2 4 184 34 211 2.26e-49 166
MS.gene52943.t1 MTR_8g009080 46.961 181 93 2 4 184 34 211 3.25e-49 165
MS.gene52943.t1 MTR_8g009080 46.961 181 93 2 4 184 34 211 1.36e-48 165
MS.gene52943.t1 MTR_8g009080 50.000 100 46 1 165 260 298 397 2.98e-24 100
MS.gene52943.t1 MTR_8g041620 42.703 185 99 4 12 194 16 195 1.72e-48 164
MS.gene52943.t1 MTR_8g041620 50.549 91 44 1 171 260 284 374 1.31e-22 95.9
MS.gene52943.t1 MTR_1g070185 43.915 189 101 3 8 196 13 196 2.02e-48 164
MS.gene52943.t1 MTR_1g070185 48.235 85 39 2 177 260 287 367 1.49e-15 75.9
MS.gene52943.t1 MTR_8g028435 45.562 169 82 5 18 184 17 177 3.89e-48 163
MS.gene52943.t1 MTR_8g028435 52.222 90 43 0 171 260 275 364 1.61e-25 103
MS.gene52943.t1 MTR_8g041600 44.311 167 88 3 12 176 16 179 1.38e-47 160
MS.gene52943.t1 MTR_8g008930 48.148 162 74 5 18 176 17 171 1.46e-47 162
MS.gene52943.t1 MTR_8g008930 48.889 90 46 0 171 260 276 365 2.43e-23 97.4
MS.gene52943.t1 MTR_8g009090 44.944 178 88 5 18 193 17 186 3.64e-47 160
MS.gene52943.t1 MTR_8g009090 50.000 90 45 0 171 260 275 364 3.51e-24 99.8
MS.gene52943.t1 MTR_8g041600 44.311 167 88 3 12 176 16 179 1.11e-46 160
MS.gene52943.t1 MTR_8g041600 45.312 128 58 3 145 260 247 374 3.99e-25 102
MS.gene52943.t1 MTR_2g069210 39.583 192 112 3 1 192 1 188 2.50e-46 159
MS.gene52943.t1 MTR_2g069210 53.535 99 39 1 169 260 269 367 3.52e-25 102
MS.gene52943.t1 MTR_8g009160 43.636 165 84 4 17 179 16 173 9.50e-46 157
MS.gene52943.t1 MTR_8g009160 50.000 90 45 0 171 260 275 364 7.09e-26 104
MS.gene52943.t1 MTR_1g043770 41.436 181 95 5 14 192 5 176 1.68e-44 152
MS.gene52943.t1 MTR_1g043770 40.884 181 96 5 14 192 5 176 3.06e-44 153
MS.gene52943.t1 MTR_1g043770 45.556 90 49 0 171 260 266 355 6.11e-20 88.2
MS.gene52943.t1 MTR_0032s0040 42.564 195 98 5 5 191 4 192 5.04e-44 153
MS.gene52943.t1 MTR_0032s0040 50.549 91 44 1 171 260 283 373 4.14e-21 91.7
MS.gene52943.t1 MTR_1g043760 44.382 178 93 4 14 191 5 176 6.23e-44 152
MS.gene52943.t1 MTR_1g043760 46.739 92 42 1 169 253 258 349 1.24e-16 78.6
MS.gene52943.t1 MTR_8g009170 46.296 162 77 5 18 176 17 171 1.38e-43 149
MS.gene52943.t1 MTR_4g117880 43.333 180 91 6 17 193 16 187 2.85e-43 150
MS.gene52943.t1 MTR_4g117880 53.623 69 32 0 171 239 276 344 3.19e-18 83.2
MS.gene52943.t1 MTR_8g009170 45.294 170 82 6 18 184 17 178 4.69e-43 150
MS.gene52943.t1 MTR_8g009170 50.549 91 44 1 171 260 276 366 2.95e-25 102
MS.gene52943.t1 MTR_2g069300 39.766 171 100 2 14 184 4 171 7.38e-43 149
MS.gene52943.t1 MTR_2g069300 53.333 90 42 0 171 260 269 358 3.71e-23 97.1
MS.gene52943.t1 MTR_1g044105 43.275 171 90 5 14 180 5 172 9.03e-43 144
MS.gene52943.t1 MTR_2g069060 42.373 177 90 2 42 218 2 166 1.65e-42 145
MS.gene52943.t1 MTR_8g009110 42.105 171 88 6 17 184 16 178 1.90e-42 148
MS.gene52943.t1 MTR_8g009110 51.724 87 42 0 174 260 279 365 4.07e-25 102
MS.gene52943.t1 MTR_8g009120 43.114 167 87 4 18 182 17 177 2.65e-42 147
MS.gene52943.t1 MTR_8g009120 54.545 77 35 0 184 260 277 353 5.18e-21 90.9
MS.gene52943.t1 MTR_2g069190 40.782 179 102 3 14 192 18 192 7.72e-42 147
MS.gene52943.t1 MTR_2g069190 50.505 99 42 1 169 260 273 371 4.00e-24 99.8
MS.gene52943.t1 MTR_8g009150 43.478 161 83 4 18 176 17 171 3.04e-41 145
MS.gene52943.t1 MTR_8g009150 51.111 90 44 0 171 260 256 345 2.79e-24 99.8
MS.gene52943.t1 MTR_7g029655 39.444 180 101 3 5 181 4 178 8.10e-41 144
MS.gene52943.t1 MTR_7g029655 48.544 103 46 3 165 260 268 370 1.98e-19 86.7
MS.gene52943.t1 MTR_7g105070 41.111 180 102 3 10 189 5 180 1.34e-40 144
MS.gene52943.t1 MTR_2g069090 40.936 171 97 3 14 184 18 184 2.81e-40 143
MS.gene52943.t1 MTR_2g069090 52.222 90 43 0 171 260 283 372 2.10e-24 100
MS.gene52943.t1 MTR_1g043810 38.636 176 104 2 14 189 5 176 5.47e-40 141
MS.gene52943.t1 MTR_1g043810 38.636 176 104 2 14 189 5 176 9.34e-40 141
MS.gene52943.t1 MTR_1g043810 50.000 84 42 0 177 260 274 357 2.91e-19 86.3
MS.gene52943.t1 MTR_1g043775 41.341 179 95 6 14 189 5 176 1.38e-38 137
MS.gene52943.t1 MTR_1g043775 41.341 179 95 6 14 189 5 176 2.72e-38 137
MS.gene52943.t1 MTR_1g043775 47.619 84 44 0 177 260 274 357 2.61e-17 80.5
MS.gene52943.t1 MTR_4g099390 64.286 84 28 1 179 260 1 84 3.25e-33 116
MS.gene52943.t1 MTR_1g032220 40.230 174 95 5 13 184 21 187 9.66e-33 123
MS.gene52943.t1 MTR_7g056353 36.667 180 81 5 12 191 18 164 3.44e-32 123
MS.gene52943.t1 MTR_7g056353 55.435 92 38 1 171 259 255 346 4.91e-26 106
MS.gene52943.t1 MTR_1g032250 36.813 182 110 2 3 184 11 187 1.23e-31 120
MS.gene52943.t1 MTR_1g032140 38.857 175 102 2 9 183 10 179 1.64e-30 117
MS.gene52943.t1 MTR_8g009180 50.588 85 42 0 171 255 205 289 2.07e-23 96.7
MS.gene52943.t1 MTR_8g008960 51.389 72 35 0 189 260 31 102 5.18e-21 85.5
MS.gene52943.t1 MTR_2g069130 50.526 95 41 2 71 163 12 102 1.63e-18 79.7
MS.gene52943.t1 MTR_1g043840 29.762 252 131 12 14 246 5 229 3.19e-17 80.1
MS.gene52943.t1 MTR_1g043840 40.964 83 46 2 78 158 257 338 1.43e-14 72.8
MS.gene52943.t1 MTR_1g032230 33.333 150 76 2 5 154 7 132 1.33e-14 72.0
MS.gene52943.t1 MTR_3g091350 28.889 180 110 7 8 175 2 175 1.59e-14 72.8
MS.gene52943.t1 MTR_8g024120 31.250 112 75 1 56 167 31 140 4.88e-14 71.2
MS.gene52943.t1 MTR_7g102820 30.864 162 102 5 14 169 10 167 5.08e-14 71.2
MS.gene52943.t1 MTR_5g032870 39.326 89 54 0 57 145 28 116 8.85e-14 70.5
MS.gene52943.t1 MTR_3g092890 29.560 159 94 4 32 181 5 154 8.41e-13 67.4
MS.gene52943.t1 MTR_5g048570 35.789 95 54 2 39 133 21 108 1.71e-12 66.6
MS.gene52943.t1 MTR_5g032880 39.535 86 44 1 57 142 28 105 1.83e-12 67.4
MS.gene52943.t1 MTR_1g062110 31.757 148 88 5 28 169 26 166 2.13e-12 66.6
MS.gene52943.t1 MTR_5g065010 29.730 111 77 1 67 176 39 149 2.63e-12 66.2
MS.gene52943.t1 MTR_1g062110 31.757 148 88 5 28 169 26 166 2.73e-12 65.9
MS.gene52943.t1 MTR_5g048740 33.945 109 65 3 29 137 11 112 5.53e-12 65.1
MS.gene52943.t1 MTR_5g055820 28.671 143 89 5 40 178 22 155 9.62e-12 64.7
MS.gene52943.t1 MTR_3g091360 29.286 140 86 6 35 167 21 154 1.41e-11 63.9
MS.gene52943.t1 MTR_1g048940 27.132 129 88 2 40 167 22 145 2.09e-11 63.5
MS.gene52943.t1 MTR_7g068650 28.571 161 96 6 20 171 6 156 5.36e-11 62.4
MS.gene52943.t1 MTR_7g081710 33.036 112 71 2 56 167 41 148 5.49e-11 62.4
MS.gene52943.t1 MTR_4g087100 34.848 132 60 7 47 169 14 128 8.52e-11 61.6
MS.gene52943.t1 MTR_5g048550 30.070 143 87 5 40 178 22 155 8.82e-11 61.6
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene52943.t1 AT1G06620 49.425 174 85 2 3 176 1 171 3.32e-55 179
MS.gene52943.t1 AT1G06620 49.425 174 85 2 3 176 1 171 2.96e-54 179
MS.gene52943.t1 AT1G06620 54.118 85 39 0 171 255 276 360 7.54e-23 96.3
MS.gene52943.t1 AT2G30840 48.864 176 86 2 11 184 3 176 1.08e-52 173
MS.gene52943.t1 AT2G30840 48.864 176 86 2 11 184 3 176 5.05e-52 173
MS.gene52943.t1 AT2G30840 55.128 78 33 2 184 260 280 356 1.49e-16 78.6
MS.gene52943.t1 AT2G30840 48.864 176 86 2 11 184 3 176 5.20e-52 173
MS.gene52943.t1 AT2G30840 53.846 91 40 2 171 260 273 362 1.61e-20 89.7
MS.gene52943.t1 AT2G25450 45.349 172 90 2 13 184 5 172 7.82e-52 172
MS.gene52943.t1 AT2G25450 47.525 101 43 4 169 260 260 359 5.88e-17 79.7
MS.gene52943.t1 AT5G59540 41.346 208 106 5 13 218 9 202 3.81e-47 158
MS.gene52943.t1 AT1G03410 45.665 173 90 3 14 184 5 175 1.16e-46 159
MS.gene52943.t1 AT1G03410 38.614 101 56 3 165 260 262 361 8.45e-12 64.7
MS.gene52943.t1 AT1G03410 45.665 173 90 3 14 184 42 212 2.15e-46 159
MS.gene52943.t1 AT1G03410 38.614 101 56 3 165 260 299 398 6.69e-12 65.1
MS.gene52943.t1 AT5G59540 41.346 208 106 5 13 218 9 202 2.45e-46 159
MS.gene52943.t1 AT5G59540 58.333 84 35 0 177 260 283 366 3.12e-25 102
MS.gene52943.t1 AT1G06650 42.286 175 96 3 13 184 10 182 2.18e-45 155
MS.gene52943.t1 AT3G61400 40.884 181 99 3 9 184 5 182 3.56e-45 154
MS.gene52943.t1 AT1G06640 42.614 176 96 3 12 184 9 182 7.47e-45 152
MS.gene52943.t1 AT2G30830 43.353 173 92 3 13 184 5 172 7.51e-45 154
MS.gene52943.t1 AT2G30830 50.000 100 41 3 169 260 260 358 1.40e-21 92.8
MS.gene52943.t1 AT1G06650 42.286 175 96 3 13 184 10 182 7.66e-45 155
MS.gene52943.t1 AT1G06650 47.253 91 47 1 171 260 279 369 1.46e-19 87.0
MS.gene52943.t1 AT1G04350 40.984 183 102 3 10 191 3 180 1.01e-44 154
MS.gene52943.t1 AT1G04350 50.495 101 45 2 165 260 260 360 7.78e-20 87.8
MS.gene52943.t1 AT1G06640 42.857 175 95 3 12 183 9 181 1.34e-44 153
MS.gene52943.t1 AT3G61400 40.884 181 99 3 9 184 5 182 1.56e-44 154
MS.gene52943.t1 AT3G61400 43.023 86 48 1 171 255 281 366 2.29e-15 75.1
MS.gene52943.t1 AT1G06640 42.614 176 96 3 12 184 9 182 3.34e-44 153
MS.gene52943.t1 AT1G06640 47.253 91 47 1 171 260 279 369 1.59e-18 84.3
MS.gene52943.t1 AT1G03400 42.690 171 88 2 14 184 5 165 1.23e-42 149
MS.gene52943.t1 AT1G03400 44.706 85 45 2 177 260 268 351 1.12e-12 67.4
MS.gene52943.t1 AT1G03400 42.690 171 88 2 14 184 5 165 2.11e-42 149
MS.gene52943.t1 AT1G03400 44.706 85 45 2 177 260 296 379 1.14e-12 67.4
MS.gene52943.t1 AT5G59530 39.535 215 111 8 13 224 8 206 2.84e-42 148
MS.gene52943.t1 AT5G59530 60.714 84 33 0 177 260 281 364 1.55e-26 106
MS.gene52943.t1 AT1G06645 44.720 161 84 3 12 169 22 180 4.60e-42 148
MS.gene52943.t1 AT1G06645 45.055 91 49 1 171 260 289 379 8.21e-19 85.1
MS.gene52943.t1 AT5G43450 43.902 164 87 3 14 176 9 168 5.27e-40 140
MS.gene52943.t1 AT5G43450 43.902 164 87 3 14 176 9 168 1.87e-39 140
MS.gene52943.t1 AT5G43450 48.101 79 41 0 177 255 279 357 5.51e-15 73.9
MS.gene52943.t1 AT5G43440 43.114 167 85 5 15 176 10 171 4.17e-38 135
MS.gene52943.t1 AT5G43440 43.114 167 85 5 15 176 10 171 2.35e-37 135
MS.gene52943.t1 AT5G43440 50.000 84 42 0 177 260 282 365 2.38e-18 83.6
MS.gene52943.t1 AT1G04380 39.333 150 85 2 40 189 21 164 1.77e-32 120
MS.gene52943.t1 AT1G04380 39.333 150 85 2 40 189 21 164 7.70e-32 120
MS.gene52943.t1 AT1G04380 59.524 84 34 0 177 260 262 345 6.89e-25 101
MS.gene52943.t1 AT3G13610 27.523 218 134 8 10 212 7 215 2.79e-16 77.8
MS.gene52943.t1 AT2G36690 31.507 146 96 2 26 169 18 161 2.54e-15 75.1
MS.gene52943.t1 AT2G36690 31.507 146 96 2 26 169 18 161 2.54e-15 75.1
MS.gene52943.t1 AT1G55290 36.364 143 78 7 28 166 25 158 7.24e-15 73.9
MS.gene52943.t1 AT5G24530 29.747 158 93 4 32 180 5 153 2.76e-14 72.0
MS.gene52943.t1 AT4G10500 33.577 137 83 3 62 192 39 173 2.03e-13 69.3
MS.gene52943.t1 AT2G44800 28.667 150 104 2 18 167 5 151 6.33e-13 68.2
MS.gene52943.t1 AT1G17020 31.429 140 90 4 29 167 17 151 1.80e-12 66.6
MS.gene52943.t1 AT3G60290 33.945 109 71 1 59 167 44 151 3.57e-12 65.9
MS.gene52943.t1 AT4G10490 29.104 134 87 2 66 193 41 172 1.00e-11 64.7
MS.gene52943.t1 AT2G38240 26.415 159 105 5 21 176 4 153 3.30e-11 63.2

Find 72 sgRNAs with CRISPR-Local

Find 115 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TCTGATAACAATTCCTTTAT+TGG 0.093755 1.1:+56969598 None:intergenic
ACTCTTCCTTGCACCCTTTC+AGG 0.130980 1.1:-56969498 MS.gene52943:CDS
CATACTTGCGGTATTTCTTC+TGG 0.229972 1.1:+56970330 None:intergenic
CTACTTTCTCATGGAGTTAG+AGG 0.275776 1.1:-56970853 None:intergenic
ATCGGAGATCAAGTTCAAAT+TGG 0.289399 1.1:+56970698 None:intergenic
AAGAAAATCCACCAATCTAT+AGG 0.298151 1.1:-56969576 MS.gene52943:CDS
TGGTATTCCGGTTAGTGTTT+TGG 0.298579 1.1:-56970532 MS.gene52943:CDS
TGGCAGCAGGGTCCGTAAAA+AGG 0.305500 1.1:+56970400 None:intergenic
AAAGTTCGATATTTCTCTAA+TGG 0.338662 1.1:-56970426 MS.gene52943:CDS
TCTTCTGGATTGGGAGGATC+GGG 0.344839 1.1:+56970345 None:intergenic
TGCGGTATTTCTTCTGGATT+GGG 0.351699 1.1:+56970336 None:intergenic
AGTGATTAATCATGGTATTC+CGG 0.365484 1.1:-56970544 MS.gene52943:CDS
TTGCGGTATTTCTTCTGGAT+TGG 0.368792 1.1:+56970335 None:intergenic
CAAGGTCCTTGGTCCAATAA+AGG 0.378696 1.1:-56969611 MS.gene52943:CDS
ATATCTTGAAGGTCTATGAT+AGG 0.390808 1.1:+56970642 None:intergenic
TTCTTCTGGATTGGGAGGAT+CGG 0.396249 1.1:+56970344 None:intergenic
ATCTCATCCAAAACACTAAC+CGG 0.405079 1.1:+56970525 None:intergenic
AGAAAATCCACCAATCTATA+GGG 0.416314 1.1:-56969575 MS.gene52943:CDS
TTACGGACCCTGCTGCCAAC+TGG 0.424630 1.1:-56970395 MS.gene52943:CDS
GATTAGTGTTTATATCTTGA+AGG 0.437519 1.1:+56970631 None:intergenic
GAGTCCCTCCAGTTGGCAGC+AGG 0.447236 1.1:+56970387 None:intergenic
TCAAGTTCAAATTGGGAGAA+TGG 0.458398 1.1:+56970706 None:intergenic
ACAGTAGTATCCCTATAGAT+TGG 0.466253 1.1:+56969565 None:intergenic
TCTTAATTCACAACCTGAAA+GGG 0.469355 1.1:+56969485 None:intergenic
TATGATAGGGACACCGAACT+TGG 0.475304 1.1:+56970656 None:intergenic
GTGAAAGGCCTCTTAGAATC+TGG 0.477165 1.1:-56970759 MS.gene52943:CDS
AAGCTTGCTATGGAAACTCT+AGG 0.481857 1.1:+56969661 None:intergenic
CTTTATTGGACCAAGGACCT+TGG 0.485130 1.1:+56969612 None:intergenic
CAAGGAAACCATTCTATACA+AGG 0.487279 1.1:-56970461 MS.gene52943:CDS
TCGGAGATCAAGTTCAAATT+GGG 0.487470 1.1:+56970699 None:intergenic
TTCTTAATTCACAACCTGAA+AGG 0.491843 1.1:+56969484 None:intergenic
TTCTAAGAGGCCTTTCACGC+CGG 0.492137 1.1:+56970764 None:intergenic
GAAGATTCGAAGTGCATGTA+AGG 0.497098 1.1:-56970583 MS.gene52943:CDS
TACTACTGTGAAAGAAGTCT+TGG 0.498074 1.1:-56969551 MS.gene52943:CDS
AGTGAAGCATCTTCCAAGTT+CGG 0.515387 1.1:-56970669 MS.gene52943:CDS
TTCCGCTGTTCTATCATATG+TGG 0.516063 1.1:+56970812 None:intergenic
TATCTTGAAGGTCTATGATA+GGG 0.518400 1.1:+56970643 None:intergenic
TACATATTCCAGATTCTAAG+AGG 0.531431 1.1:+56970751 None:intergenic
GAGGTTACATCCAAGGTCCT+TGG 0.536404 1.1:-56969622 MS.gene52943:CDS
AGTCCCTCCAGTTGGCAGCA+GGG 0.536596 1.1:+56970388 None:intergenic
TAATCCATGTTTACATGTTG+AGG 0.536783 1.1:-56970613 MS.gene52943:CDS
AGATGCTTCACTTTCATGAT+CGG 0.537892 1.1:+56970680 None:intergenic
TCGAAGTGCATGTAAGGAGT+GGG 0.538796 1.1:-56970577 MS.gene52943:CDS
TGTTCACAAGTGTTTACCAC+CGG 0.539446 1.1:-56969705 MS.gene52943:CDS
GTTTGCAAAGAAGCTTGCTA+TGG 0.540340 1.1:+56969651 None:intergenic
CTTCTTTGCAAACTCAGTAG+AGG 0.552479 1.1:-56969641 MS.gene52943:CDS
CTTCTGGATTGGGAGGATCG+GGG 0.565105 1.1:+56970346 None:intergenic
GCAAGGAAGAGTTCCCATCA+AGG 0.565323 1.1:+56969509 None:intergenic
CATCTTGTTCATGAAACCTA+CGG 0.566748 1.1:+56970490 None:intergenic
TGTTCTATCATATGTGGAAG+TGG 0.581025 1.1:+56970818 None:intergenic
CTCAGTAGAGGTTACATCCA+AGG 0.583457 1.1:-56969629 MS.gene52943:CDS
CGGACCCTGCTGCCAACTGG+AGG 0.587707 1.1:-56970392 MS.gene52943:CDS
ATGAACAAGATGCTGATGCA+AGG 0.589480 1.1:-56970479 MS.gene52943:CDS
TCACAACCTGAAAGGGTGCA+AGG 0.591043 1.1:+56969492 None:intergenic
TTCGAAGTGCATGTAAGGAG+TGG 0.595798 1.1:-56970578 MS.gene52943:CDS
AGCTTGCTATGGAAACTCTA+GGG 0.600458 1.1:+56969662 None:intergenic
CTATATGCTTCGATAAAACC+CGG 0.600515 1.1:+56969686 None:intergenic
GTAGTATCCCTATAGATTGG+TGG 0.600698 1.1:+56969568 None:intergenic
ACAATTCCTTTATTGGACCA+AGG 0.603315 1.1:+56969605 None:intergenic
GGTATTTCTTCTGGATTGGG+AGG 0.605051 1.1:+56970339 None:intergenic
TGAAAGTGAGTCCCTCCAGT+TGG 0.622368 1.1:+56970380 None:intergenic
GGACCCTGCTGCCAACTGGA+GGG 0.624510 1.1:-56970391 MS.gene52943:CDS
ATGGAGTTAGAGGCAAGTAA+TGG 0.628115 1.1:-56970843 MS.gene52943:CDS
AGTACCTCAACATGTAAACA+TGG 0.631590 1.1:+56970609 None:intergenic
AGATGATTAGTGGAATCCGT+AGG 0.632212 1.1:-56970506 MS.gene52943:CDS
GTTCACAAGTGTTTACCACC+GGG 0.650598 1.1:-56969704 MS.gene52943:CDS
CGAAGTGCATGTAAGGAGTG+GGG 0.653285 1.1:-56970576 MS.gene52943:CDS
TGGGAGAATGGAACATACGT+GGG 0.666519 1.1:+56970718 None:intergenic
AAGGAAACCATTCTATACAA+GGG 0.666588 1.1:-56970460 MS.gene52943:CDS
TTGGGAGAATGGAACATACG+TGG 0.674537 1.1:+56970717 None:intergenic
TTCCACATATGATAGAACAG+CGG 0.752551 1.1:-56970814 MS.gene52943:CDS
TATGCTTCGATAAAACCCGG+TGG 0.773902 1.1:+56969689 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! AATGTTAGTTTTATATCTTT+TGG - chr1.1:56970033-56970052 MS.gene52943:intron 15.0%
!! AATTGTCATGAAATCAATAT+CGG + chr1.1:56970392-56970411 None:intergenic 20.0%
!!! TAGTTTTATATCTTTTGGAT+CGG - chr1.1:56970038-56970057 MS.gene52943:intron 20.0%
!!! TTTTTTCAAGTGATTAATCA+TGG - chr1.1:56969783-56969802 MS.gene52943:intron 20.0%
! AAAGTTCGATATTTCTCTAA+TGG - chr1.1:56969909-56969928 MS.gene52943:intron 25.0%
! GATTAGTGTTTATATCTTGA+AGG + chr1.1:56969707-56969726 None:intergenic 25.0%
! TCTGATAACAATTCCTTTAT+TGG + chr1.1:56970740-56970759 None:intergenic 25.0%
! TTGATGAGATGATTATGTAT+TGG - chr1.1:56970172-56970191 MS.gene52943:intron 25.0%
!!! AGTTTTATATCTTTTGGATC+GGG - chr1.1:56970039-56970058 MS.gene52943:intron 25.0%
!!! TTGGCACATTATTTTACAAA+AGG - chr1.1:56970803-56970822 MS.gene52943:CDS 25.0%
AAGAAAATCCACCAATCTAT+AGG - chr1.1:56970759-56970778 MS.gene52943:CDS 30.0%
AAGGAAACCATTCTATACAA+GGG - chr1.1:56969875-56969894 MS.gene52943:intron 30.0%
AAGTTCTTCATCAGAATCAA+TGG - chr1.1:56970440-56970459 MS.gene52943:CDS 30.0%
AGAAAATCCACCAATCTATA+GGG - chr1.1:56970760-56970779 MS.gene52943:CDS 30.0%
AGAAGTTCCCATAGTTAATT+CGG + chr1.1:56970362-56970381 None:intergenic 30.0%
AGTGATTAATCATGGTATTC+CGG - chr1.1:56969791-56969810 MS.gene52943:intron 30.0%
AGTTCTTCATCAGAATCAAT+GGG - chr1.1:56970441-56970460 MS.gene52943:CDS 30.0%
ATATCTTGAAGGTCTATGAT+AGG + chr1.1:56969696-56969715 None:intergenic 30.0%
GAGAAATTGTCCATAATCTA+AGG + chr1.1:56970308-56970327 None:intergenic 30.0%
GATGTTAAGTGTAAACACTT+TGG - chr1.1:56970526-56970545 MS.gene52943:CDS 30.0%
TAATCATCTCATCAAAGACA+AGG + chr1.1:56970167-56970186 None:intergenic 30.0%
TAATCCATGTTTACATGTTG+AGG - chr1.1:56969722-56969741 MS.gene52943:CDS 30.0%
TACATATTCCAGATTCTAAG+AGG + chr1.1:56969587-56969606 None:intergenic 30.0%
TATCTTGAAGGTCTATGATA+GGG + chr1.1:56969695-56969714 None:intergenic 30.0%
TCAAAGAAAGTAAGAGCTTT+AGG - chr1.1:56970217-56970236 MS.gene52943:intron 30.0%
! ATTTTACAAAAGGCCTTGAT+GGG - chr1.1:56970813-56970832 MS.gene52943:CDS 30.0%
! CTTTTATCCCTTGTATAGAA+TGG + chr1.1:56969885-56969904 None:intergenic 30.0%
! TATTTTACAAAAGGCCTTGA+TGG - chr1.1:56970812-56970831 MS.gene52943:CDS 30.0%
ACAATTCCTTTATTGGACCA+AGG + chr1.1:56970733-56970752 None:intergenic 35.0%
ACAGTAGTATCCCTATAGAT+TGG + chr1.1:56970773-56970792 None:intergenic 35.0%
AGAATAGCTCAAAGATTGTC+AGG + chr1.1:56970243-56970262 None:intergenic 35.0%
AGATGCTTCACTTTCATGAT+CGG + chr1.1:56969658-56969677 None:intergenic 35.0%
AGTACCTCAACATGTAAACA+TGG + chr1.1:56969729-56969748 None:intergenic 35.0%
ATCGGAGATCAAGTTCAAAT+TGG + chr1.1:56969640-56969659 None:intergenic 35.0%
ATCTCATCCAAAACACTAAC+CGG + chr1.1:56969813-56969832 None:intergenic 35.0%
ATTCTTCTACAAGACCAGAT+TGG - chr1.1:56970409-56970428 MS.gene52943:CDS 35.0%
CAAGGAAACCATTCTATACA+AGG - chr1.1:56969874-56969893 MS.gene52943:intron 35.0%
CATCTTGTTCATGAAACCTA+CGG + chr1.1:56969848-56969867 None:intergenic 35.0%
CTATATGCTTCGATAAAACC+CGG + chr1.1:56970652-56970671 None:intergenic 35.0%
GAAGTTCCCATAGTTAATTC+GGG + chr1.1:56970361-56970380 None:intergenic 35.0%
TAACATTACCTGCATACTTG+CGG + chr1.1:56970020-56970039 None:intergenic 35.0%
TACTACTGTGAAAGAAGTCT+TGG - chr1.1:56970784-56970803 MS.gene52943:CDS 35.0%
TCAAGTTCAAATTGGGAGAA+TGG + chr1.1:56969632-56969651 None:intergenic 35.0%
TCCTTCTTACCTTAGAGATT+TGG - chr1.1:56970282-56970301 MS.gene52943:intron 35.0%
TCGGAGATCAAGTTCAAATT+GGG + chr1.1:56969639-56969658 None:intergenic 35.0%
TGTTCTATCATATGTGGAAG+TGG + chr1.1:56969520-56969539 None:intergenic 35.0%
TTCCACATATGATAGAACAG+CGG - chr1.1:56969521-56969540 MS.gene52943:CDS 35.0%
! AATCTAAGGCCAAATCTCTA+AGG + chr1.1:56970294-56970313 None:intergenic 35.0%
! CTCTAATGGTAGCCTTTTTA+CGG - chr1.1:56969923-56969942 MS.gene52943:intron 35.0%
! GCCTTTGAAGAATCAAAAAC+CGG - chr1.1:56969552-56969571 MS.gene52943:CDS 35.0%
! GGATCTCTTGTTGTAAACAT+TGG - chr1.1:56970484-56970503 MS.gene52943:CDS 35.0%
!! AGAGATTTGGCCTTAGATTA+TGG - chr1.1:56970295-56970314 MS.gene52943:intron 35.0%
!! GTTTTGGATGAGATGATTAG+TGG - chr1.1:56969819-56969838 MS.gene52943:intron 35.0%
AAGAAATACCGCAAGTATGC+AGG - chr1.1:56970009-56970028 MS.gene52943:intron 40.0%
AGATGATTAGTGGAATCCGT+AGG - chr1.1:56969829-56969848 MS.gene52943:intron 40.0%
ATGAACAAGATGCTGATGCA+AGG - chr1.1:56969856-56969875 MS.gene52943:intron 40.0%
ATGGAGTTAGAGGCAAGTAA+TGG - chr1.1:56969492-56969511 MS.gene52943:CDS 40.0%
CATACTTGCGGTATTTCTTC+TGG + chr1.1:56970008-56970027 None:intergenic 40.0%
CCTGAACCCGAATTAACTAT+GGG - chr1.1:56970352-56970371 MS.gene52943:CDS 40.0%
CTTCTTTGCAAACTCAGTAG+AGG - chr1.1:56970694-56970713 MS.gene52943:CDS 40.0%
GAAGATTCGAAGTGCATGTA+AGG - chr1.1:56969752-56969771 MS.gene52943:intron 40.0%
GAGCTATTCTCAGAAGCTTT+AGG - chr1.1:56970253-56970272 MS.gene52943:intron 40.0%
GTAGTATCCCTATAGATTGG+TGG + chr1.1:56970770-56970789 None:intergenic 40.0%
TGCGGTATTTCTTCTGGATT+GGG + chr1.1:56970002-56970021 None:intergenic 40.0%
TGTTCACAAGTGTTTACCAC+CGG - chr1.1:56970630-56970649 MS.gene52943:CDS 40.0%
TTCCGCTGTTCTATCATATG+TGG + chr1.1:56969526-56969545 None:intergenic 40.0%
TTGCGGTATTTCTTCTGGAT+TGG + chr1.1:56970003-56970022 None:intergenic 40.0%
! AAACATTGGTGATCTTCTGC+AGG - chr1.1:56970498-56970517 MS.gene52943:CDS 40.0%
! AGTGAAGCATCTTCCAAGTT+CGG - chr1.1:56969666-56969685 MS.gene52943:CDS 40.0%
! CCATAGTTAATTCGGGTTCA+GGG + chr1.1:56970354-56970373 None:intergenic 40.0%
! TGGTATTCCGGTTAGTGTTT+TGG - chr1.1:56969803-56969822 MS.gene52943:intron 40.0%
!! AAGCTTGCTATGGAAACTCT+AGG + chr1.1:56970677-56970696 None:intergenic 40.0%
!! AGCTTGCTATGGAAACTCTA+GGG + chr1.1:56970676-56970695 None:intergenic 40.0%
!! CGGGTTTTATCGAAGCATAT+AGG - chr1.1:56970650-56970669 MS.gene52943:CDS 40.0%
!! CTCTAAGGTAAGAAGGATCA+AGG + chr1.1:56970279-56970298 None:intergenic 40.0%
!! GCCAAATCTCTAAGGTAAGA+AGG + chr1.1:56970286-56970305 None:intergenic 40.0%
!! GCCGGTTTTTGATTCTTCAA+AGG + chr1.1:56969556-56969575 None:intergenic 40.0%
!! GTTTGCAAAGAAGCTTGCTA+TGG + chr1.1:56970687-56970706 None:intergenic 40.0%
ACAACAAGAGATCCATGCAG+AGG + chr1.1:56970478-56970497 None:intergenic 45.0%
CAAGGTCCTTGGTCCAATAA+AGG - chr1.1:56970724-56970743 MS.gene52943:CDS 45.0%
CCCTGAACCCGAATTAACTA+TGG - chr1.1:56970351-56970370 MS.gene52943:CDS 45.0%
CTCAGTAGAGGTTACATCCA+AGG - chr1.1:56970706-56970725 MS.gene52943:CDS 45.0%
CTTCTACAAGACCAGATTGG+TGG - chr1.1:56970412-56970431 MS.gene52943:CDS 45.0%
CTTTATTGGACCAAGGACCT+TGG + chr1.1:56970726-56970745 None:intergenic 45.0%
GAACTTGAAGACCACCAATC+TGG + chr1.1:56970426-56970445 None:intergenic 45.0%
GAATCAAAAACCGGCGTGAA+AGG - chr1.1:56969561-56969580 MS.gene52943:CDS 45.0%
GTGAAAGGCCTCTTAGAATC+TGG - chr1.1:56969576-56969595 MS.gene52943:CDS 45.0%
GTTCACAAGTGTTTACCACC+GGG - chr1.1:56970631-56970650 MS.gene52943:CDS 45.0%
TATGATAGGGACACCGAACT+TGG + chr1.1:56969682-56969701 None:intergenic 45.0%
TATGCTTCGATAAAACCCGG+TGG + chr1.1:56970649-56970668 None:intergenic 45.0%
TCGAAGTGCATGTAAGGAGT+GGG - chr1.1:56969758-56969777 MS.gene52943:intron 45.0%
TGGGAGAATGGAACATACGT+GGG + chr1.1:56969620-56969639 None:intergenic 45.0%
TTCGAAGTGCATGTAAGGAG+TGG - chr1.1:56969757-56969776 MS.gene52943:intron 45.0%
TTGGGAGAATGGAACATACG+TGG + chr1.1:56969621-56969640 None:intergenic 45.0%
! CCCATAGTTAATTCGGGTTC+AGG + chr1.1:56970355-56970374 None:intergenic 45.0%
! GGTATTTCTTCTGGATTGGG+AGG + chr1.1:56969999-56970018 None:intergenic 45.0%
! TTCTTCTGGATTGGGAGGAT+CGG + chr1.1:56969994-56970013 None:intergenic 45.0%
ACAAGAGATCCATGCAGAGG+AGG + chr1.1:56970475-56970494 None:intergenic 50.0%
ACTCTTCCTTGCACCCTTTC+AGG - chr1.1:56970837-56970856 MS.gene52943:CDS 50.0%
CGAAGTGCATGTAAGGAGTG+GGG - chr1.1:56969759-56969778 MS.gene52943:intron 50.0%
GAGGTTACATCCAAGGTCCT+TGG - chr1.1:56970713-56970732 MS.gene52943:CDS 50.0%
GCAAGGAAGAGTTCCCATCA+AGG + chr1.1:56970829-56970848 None:intergenic 50.0%
GTTGATGTTCCTCCTCTGCA+TGG - chr1.1:56970463-56970482 MS.gene52943:CDS 50.0%
TGAAAGTGAGTCCCTCCAGT+TGG + chr1.1:56969958-56969977 None:intergenic 50.0%
! TCACAACCTGAAAGGGTGCA+AGG + chr1.1:56970846-56970865 None:intergenic 50.0%
! TCTTCTGGATTGGGAGGATC+GGG + chr1.1:56969993-56970012 None:intergenic 50.0%
! TTCTAAGAGGCCTTTCACGC+CGG + chr1.1:56969574-56969593 None:intergenic 50.0%
TGGCAGCAGGGTCCGTAAAA+AGG + chr1.1:56969938-56969957 None:intergenic 55.0%
! AATTCGGGTTCAGGGCATGC+AGG + chr1.1:56970346-56970365 None:intergenic 55.0%
! CTTCTGGATTGGGAGGATCG+GGG + chr1.1:56969992-56970011 None:intergenic 55.0%
AGTCCCTCCAGTTGGCAGCA+GGG + chr1.1:56969950-56969969 None:intergenic 60.0%
TTACGGACCCTGCTGCCAAC+TGG - chr1.1:56969940-56969959 MS.gene52943:intron 60.0%
GAGTCCCTCCAGTTGGCAGC+AGG + chr1.1:56969951-56969970 None:intergenic 65.0%
GGACCCTGCTGCCAACTGGA+GGG - chr1.1:56969944-56969963 MS.gene52943:intron 65.0%
CGGACCCTGCTGCCAACTGG+AGG - chr1.1:56969943-56969962 MS.gene52943:intron 70.0%


Chromosome Type Strat End Strand Name
chr1.1 gene 56969492 56970865 56969492 ID=MS.gene52943
chr1.1 mRNA 56969492 56970865 56969492 ID=MS.gene52943.t1;Parent=MS.gene52943
chr1.1 exon 56970335 56970865 56970335 ID=MS.gene52943.t1.exon1;Parent=MS.gene52943.t1
chr1.1 CDS 56970335 56970865 56970335 ID=cds.MS.gene52943.t1;Parent=MS.gene52943.t1
chr1.1 exon 56969492 56969743 56969492 ID=MS.gene52943.t1.exon2;Parent=MS.gene52943.t1
chr1.1 CDS 56969492 56969743 56969492 ID=cds.MS.gene52943.t1;Parent=MS.gene52943.t1
Gene Sequence

>MS.gene52943

ATGGAGTTAGAGGCAAGTAATGGTTCCACTTCCACATATGATAGAACAGCGGAAGTGAAAGCCTTTGAAGAATCAAAAACCGGCGTGAAAGGCCTCTTAGAATCTGGAATATGTACGACAAAGATCCCACGTATGTTCCATTCTCCCAATTTGAACTTGATCTCCGATCATGAAAGTGAAGCATCTTCCAAGTTCGGTGTCCCTATCATAGACCTTCAAGATATAAACACTAATCCATGTTTACATGTTGAGGTACTTGAGAAGATTCGAAGTGCATGTAAGGAGTGGGGATTTTTTCAAGTGATTAATCATGGTATTCCGGTTAGTGTTTTGGATGAGATGATTAGTGGAATCCGTAGGTTTCATGAACAAGATGCTGATGCAAGGAAACCATTCTATACAAGGGATAAAAGTAAGAAAGTTCGATATTTCTCTAATGGTAGCCTTTTTACGGACCCTGCTGCCAACTGGAGGGACTCACTTTCATTTTTTGTAAGCCCCGATCCTCCCAATCCAGAAGAAATACCGCAAGTATGCAGGTAATGTTAGTTTTATATCTTTTGGATCGGGCAAAGTTATGTCACTTAAAGATCAATCACTAAGTTTAATTACAAGTTTTTCTTAAACTACACTTATAAGTTTTTCTTAAATTTCACTTCTGTATGTTTTTTTCCTTGTCTTTGATGAGATGATTATGTATTGGCAGAGACATTGTGATTGAATATTCAAAGAAAGTAAGAGCTTTAGGCCTGACAATCTTTGAGCTATTCTCAGAAGCTTTAGGCCTTGATCCTTCTTACCTTAGAGATTTGGCCTTAGATTATGGACAATTTCTCTTGTGTCACTACTATCCTGCATGCCCTGAACCCGAATTAACTATGGGAACTTCTAAGCACACCGATATTGATTTCATGACAATTCTTCTACAAGACCAGATTGGTGGTCTTCAAGTTCTTCATCAGAATCAATGGGTTGATGTTCCTCCTCTGCATGGATCTCTTGTTGTAAACATTGGTGATCTTCTGCAGGTAAGCGATGTTAAGTGTAAACACTTTGGTTTTTTCTGTACTGTCTTGTGACATTTAATTTAATAAAGATTACATTTATCAATTTTCTTTGCAGCTTGTAACAAATGACATGTTCACAAGTGTTTACCACCGGGTTTTATCGAAGCATATAGGCCCTAGAGTTTCCATAGCAAGCTTCTTTGCAAACTCAGTAGAGGTTACATCCAAGGTCCTTGGTCCAATAAAGGAATTGTTATCAGAAGAAAATCCACCAATCTATAGGGATACTACTGTGAAAGAAGTCTTGGCACATTATTTTACAAAAGGCCTTGATGGGAACTCTTCCTTGCACCCTTTCAGGTTGTGA
Protein sequence

>MS.gene52943.t1

MELEASNGSTSTYDRTAEVKAFEESKTGVKGLLESGICTTKIPRMFHSPNLNLISDHESEASSKFGVPIIDLQDINTNPCLHVEVLEKIRSACKEWGFFQVINHGIPVSVLDEMISGIRRFHEQDADARKPFYTRDKSKKVRYFSNGSLFTDPAANWRDSLSFFVSPDPPNPEEIPQLVTNDMFTSVYHRVLSKHIGPRVSIASFFANSVEVTSKVLGPIKELLSEENPPIYRDTTVKEVLAHYFTKGLDGNSSLHPFRL