AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene52991

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene52991.t1 MTR_1g075440 98.214 112 2 0 1 112 1 112 8.03e-74 230
MS.gene52991.t1 MTR_1g041815 83.784 111 17 1 1 111 1 110 1.37e-60 196
MS.gene52991.t1 MTR_1g041835 81.982 111 19 1 1 111 1 110 5.75e-60 194
MS.gene52991.t1 MTR_1g042310 84.314 102 16 0 10 111 8 109 4.34e-58 189
MS.gene52991.t1 MTR_1g041875 75.000 112 27 1 1 112 3 113 3.58e-56 184
MS.gene52991.t1 MTR_7g098160 74.038 104 27 0 8 111 13 116 2.30e-54 179
MS.gene52991.t1 MTR_7g098180 70.270 111 33 0 1 111 4 114 1.67e-53 177
MS.gene52991.t1 MTR_7g098150 72.115 104 29 0 8 111 13 116 4.47e-53 174
MS.gene52991.t1 MTR_7g098090 72.115 104 29 0 8 111 13 116 4.69e-53 176
MS.gene52991.t1 MTR_7g098220 66.364 110 35 1 2 111 6 113 1.16e-49 167
MS.gene52991.t1 MTR_7g098040 69.903 103 31 0 8 110 14 116 1.46e-49 167
MS.gene52991.t1 MTR_7g098200 68.932 103 32 0 8 110 9 111 9.12e-49 164
MS.gene52991.t1 MTR_7g098200 68.932 103 32 0 8 110 9 111 9.15e-49 164
MS.gene52991.t1 MTR_3g072300 60.577 104 41 0 8 111 4 107 4.80e-43 149
MS.gene52991.t1 MTR_1g042310 80.556 72 14 0 40 111 1 72 1.18e-35 128
MS.gene52991.t1 MTR_3g462710 54.630 108 43 2 1 108 1 102 1.30e-34 125
MS.gene52991.t1 MTR_1g041885 81.818 66 12 0 47 112 1 66 2.82e-33 122
MS.gene52991.t1 MTR_1g042310 83.077 65 11 0 47 111 1 65 1.17e-32 120
MS.gene52991.t1 MTR_3g005010 53.211 109 49 1 3 111 7 113 1.20e-32 120
MS.gene52991.t1 MTR_7g098230 71.429 70 20 0 41 110 39 108 3.05e-31 116
MS.gene52991.t1 MTR_7g067010 47.115 104 55 0 8 111 4 107 2.75e-30 114
MS.gene52991.t1 MTR_4g015080 49.074 108 52 1 7 111 10 117 3.17e-30 113
MS.gene52991.t1 MTR_7g098220 65.217 69 24 0 43 111 1 69 1.26e-28 108
MS.gene52991.t1 MTR_4g033395 47.959 98 51 0 14 111 18 115 3.55e-27 104
MS.gene52991.t1 MTR_4g033375 46.939 98 52 0 14 111 18 115 2.00e-26 102
MS.gene52991.t1 MTR_3g069500 45.192 104 57 0 8 111 15 118 7.93e-26 101
MS.gene52991.t1 MTR_3g069420 41.905 105 61 0 8 112 14 118 9.82e-26 101
MS.gene52991.t1 MTR_0294s0020 48.485 99 51 0 9 107 12 110 1.45e-25 100
MS.gene52991.t1 MTR_4g033330 48.936 94 48 0 14 107 17 110 8.66e-25 98.2
MS.gene52991.t1 MTR_4g033310 48.936 94 48 0 14 107 17 110 1.18e-24 97.8
MS.gene52991.t1 MTR_4g033325 46.809 94 50 0 14 107 17 110 1.73e-24 97.4
MS.gene52991.t1 MTR_4g033315 48.936 94 48 0 14 107 17 110 1.85e-24 97.4
MS.gene52991.t1 MTR_4g033310 48.936 94 48 0 14 107 17 110 1.85e-24 97.4
MS.gene52991.t1 MTR_7g065080 39.474 114 66 1 1 111 1 114 2.87e-24 97.1
MS.gene52991.t1 MTR_3g069440 45.745 94 51 0 14 107 19 112 3.27e-22 91.3
MS.gene52991.t1 MTR_4g019580 38.235 102 63 0 10 111 18 119 5.70e-22 90.5
MS.gene52991.t1 MTR_8g038880 50.588 85 42 0 27 111 19 103 7.11e-22 90.1
MS.gene52991.t1 MTR_5g014150 34.951 103 67 0 9 111 25 127 9.23e-22 89.7
MS.gene52991.t1 MTR_3g112460 37.755 98 61 0 13 110 8 105 3.90e-21 88.2
MS.gene52991.t1 MTR_3g014790 41.414 99 58 0 13 111 2 100 4.03e-20 85.1
MS.gene52991.t1 MTR_7g006540 43.269 104 57 2 11 112 44 147 5.38e-20 84.7
MS.gene52991.t1 MTR_4g098800 35.577 104 67 0 9 112 17 120 6.90e-20 84.3
MS.gene52991.t1 MTR_3g112470 38.776 98 60 0 13 110 8 105 6.91e-20 84.3
MS.gene52991.t1 MTR_4g024930 33.333 99 66 0 12 110 20 118 7.04e-20 84.3
MS.gene52991.t1 MTR_4g098800 35.577 104 67 0 9 112 82 185 7.80e-20 84.3
MS.gene52991.t1 MTR_7g010820 35.922 103 66 0 9 111 7 109 1.87e-19 83.2
MS.gene52991.t1 MTR_5g012290 43.182 88 50 0 18 105 17 104 6.74e-19 81.6
MS.gene52991.t1 MTR_4g101380 42.045 88 51 0 18 105 17 104 1.04e-18 81.3
MS.gene52991.t1 MTR_7g088825 37.500 104 65 0 7 110 1 104 1.62e-18 80.5
MS.gene52991.t1 MTR_3g112450 38.298 94 58 0 17 110 12 105 2.68e-18 80.1
MS.gene52991.t1 MTR_5g012270 37.374 99 56 1 2 100 9 101 5.74e-18 79.0
MS.gene52991.t1 MTR_4g107670 39.450 109 62 1 1 109 1 105 7.09e-18 78.6
MS.gene52991.t1 MTR_7g088820 39.450 109 59 2 2 110 6 107 1.38e-17 77.8
MS.gene52991.t1 MTR_5g038060 41.860 86 50 0 26 111 29 114 4.38e-17 76.6
MS.gene52991.t1 MTR_5g055000 39.560 91 55 0 20 110 10 100 8.92e-17 75.5
MS.gene52991.t1 MTR_7g088790 40.426 94 56 0 17 110 18 111 1.04e-16 75.5
MS.gene52991.t1 MTR_8g469480 41.975 81 47 0 31 111 41 121 1.13e-16 75.1
MS.gene52991.t1 MTR_7g013270 35.227 88 57 0 18 105 80 167 1.85e-16 74.7
MS.gene52991.t1 MTR_2g005130 37.500 104 65 0 9 112 21 124 2.24e-16 74.3
MS.gene52991.t1 MTR_5g038380 39.796 98 59 0 14 111 14 111 8.86e-16 72.8
MS.gene52991.t1 MTR_1g116930 32.692 104 69 1 8 111 4 106 8.88e-16 72.8
MS.gene52991.t1 MTR_7g498330 65.909 44 14 1 8 51 9 51 7.24e-15 64.3
MS.gene52991.t1 MTR_8g469470 29.808 104 73 0 8 111 12 115 7.63e-15 70.1
MS.gene52991.t1 MTR_7g098080 57.143 49 18 1 33 78 13 61 1.05e-14 64.3
MS.gene52991.t1 MTR_1g026750 33.333 93 60 1 14 104 2 94 1.12e-14 69.7
MS.gene52991.t1 MTR_7g005930 34.906 106 66 2 10 114 19 122 1.30e-14 69.3
MS.gene52991.t1 MTR_4g114340 39.216 102 62 0 8 109 4 105 2.12e-14 68.9
MS.gene52991.t1 MTR_7g098210 48.438 64 18 1 47 110 1 49 5.58e-14 67.4
MS.gene52991.t1 MTR_1g026740 36.585 82 52 0 30 111 19 100 9.24e-14 67.0
MS.gene52991.t1 MTR_5g038190 35.106 94 61 0 19 112 15 108 1.49e-13 66.2
MS.gene52991.t1 MTR_8g469310 37.895 95 59 0 15 109 13 107 4.81e-13 65.1
MS.gene52991.t1 MTR_1g026770 38.667 75 46 0 30 104 17 91 6.16e-13 64.7
MS.gene52991.t1 MTR_5g085850 35.294 102 66 0 8 109 10 111 1.60e-12 63.5
MS.gene52991.t1 MTR_7g064160 27.434 113 78 2 1 109 1 113 6.48e-12 58.5
MS.gene52991.t1 MTR_1g009200 31.683 101 65 1 8 108 11 107 1.35e-11 60.8
MS.gene52991.t1 MTR_8g087810 34.000 100 66 0 9 108 2 101 1.96e-11 60.5
MS.gene52991.t1 MTR_7g064780 25.000 120 82 1 8 119 6 125 2.60e-11 60.1
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene52991.t1 AT5G46040 75.238 105 26 0 7 111 6 110 7.94e-52 172
MS.gene52991.t1 AT5G46050 72.642 106 29 0 7 112 6 111 8.72e-49 164
MS.gene52991.t1 AT2G40460 58.559 111 42 1 1 111 1 107 3.05e-43 149
MS.gene52991.t1 AT2G02040 53.571 112 50 1 1 110 12 123 1.83e-36 131
MS.gene52991.t1 AT3G54140 51.786 112 50 1 1 112 1 108 5.01e-36 129
MS.gene52991.t1 AT3G54140 51.786 112 50 1 1 112 1 108 5.01e-36 129
MS.gene52991.t1 AT5G01180 50.962 104 49 1 8 111 5 106 3.91e-32 119
MS.gene52991.t1 AT5G01180 50.962 104 49 1 8 111 5 106 3.91e-32 119
MS.gene52991.t1 AT5G01180 50.962 104 49 1 8 111 5 106 3.91e-32 119
MS.gene52991.t1 AT2G02020 45.133 113 59 1 3 112 14 126 2.59e-31 116
MS.gene52991.t1 AT2G02020 45.133 113 59 1 3 112 14 126 3.23e-31 116
MS.gene52991.t1 AT1G62200 43.220 118 55 2 1 108 15 130 4.04e-28 107
MS.gene52991.t1 AT1G62200 43.220 118 55 2 1 108 15 130 4.17e-28 107
MS.gene52991.t1 AT1G62200 43.220 118 55 2 1 108 29 144 4.28e-28 107
MS.gene52991.t1 AT3G54450 55.814 86 37 1 26 111 11 95 1.39e-25 100
MS.gene52991.t1 AT1G22570 48.113 106 54 1 2 107 5 109 1.64e-25 100
MS.gene52991.t1 AT1G22550 46.226 106 56 1 2 107 5 109 8.65e-25 98.6
MS.gene52991.t1 AT1G22540 52.083 96 46 0 16 111 18 113 1.62e-24 97.8
MS.gene52991.t1 AT3G53960 41.346 104 61 0 8 111 17 120 1.01e-23 95.5
MS.gene52991.t1 AT2G37900 38.462 104 64 0 8 111 18 121 1.23e-23 95.1
MS.gene52991.t1 AT1G72120 48.571 105 53 1 7 111 9 112 1.24e-22 92.4
MS.gene52991.t1 AT1G72140 40.909 110 64 1 2 111 9 117 1.35e-22 92.0
MS.gene52991.t1 AT1G72125 50.476 105 51 1 7 111 9 112 1.58e-21 89.4
MS.gene52991.t1 AT1G32450 37.864 103 64 0 9 111 18 120 2.07e-21 89.0
MS.gene52991.t1 AT1G12110 42.391 92 53 0 18 109 17 108 1.43e-20 86.7
MS.gene52991.t1 AT4G21680 37.864 103 62 1 9 109 9 111 2.76e-20 85.5
MS.gene52991.t1 AT5G13400 43.750 96 53 1 17 111 52 147 3.42e-20 85.5
MS.gene52991.t1 AT1G59740 38.596 114 67 1 2 112 12 125 4.61e-19 82.0
MS.gene52991.t1 AT1G72130 43.182 88 50 0 24 111 21 108 5.80e-19 82.0
MS.gene52991.t1 AT1G68570 39.091 110 62 3 1 110 1 105 7.25e-18 78.6
MS.gene52991.t1 AT1G69870 40.230 87 52 0 25 111 48 134 6.84e-17 75.9
MS.gene52991.t1 AT1G22570 56.061 66 29 0 42 107 7 72 7.06e-17 75.9
MS.gene52991.t1 AT5G62730 37.500 96 60 0 14 109 42 137 1.65e-15 72.0
MS.gene52991.t1 AT1G68570 46.377 69 37 0 42 110 7 75 2.51e-15 71.6
MS.gene52991.t1 AT5G19640 36.145 83 53 0 29 111 60 142 5.14e-15 70.5
MS.gene52991.t1 AT2G26690 39.216 102 62 0 8 109 4 105 1.01e-14 69.7
MS.gene52991.t1 AT1G33440 36.036 111 65 1 9 113 19 129 2.64e-14 68.6
MS.gene52991.t1 AT5G28470 38.202 89 52 1 24 109 13 101 4.54e-14 67.8
MS.gene52991.t1 AT5G28470 38.202 89 52 1 24 109 13 101 4.76e-14 67.8
MS.gene52991.t1 AT3G25260 35.644 101 61 1 5 105 2 98 2.42e-13 65.9
MS.gene52991.t1 AT3G16180 36.364 88 55 1 22 108 16 103 3.89e-13 65.1
MS.gene52991.t1 AT1G27080 37.349 83 52 0 26 108 12 94 4.79e-13 65.1
MS.gene52991.t1 AT1G69850 34.615 104 67 1 7 109 3 106 5.50e-13 64.7
MS.gene52991.t1 AT1G52190 34.940 83 54 0 26 108 19 101 3.65e-12 62.4
MS.gene52991.t1 AT3G01350 35.632 87 55 1 25 110 3 89 1.59e-11 60.5
MS.gene52991.t1 AT1G69860 29.070 86 61 0 26 111 16 101 3.79e-11 59.7
MS.gene52991.t1 AT5G14940 33.735 83 55 0 28 110 7 89 7.46e-11 58.5

Find 35 sgRNAs with CRISPR-Local

Find 123 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TTTGAAAGAATGGCATATTA+TGG 0.240890 1.1:-56429356 MS.gene52991:CDS
ATTTGGATGACACCTATATT+AGG 0.308097 1.1:-56429242 MS.gene52991:CDS
CAACCAAGATCTTTGGCTAT+TGG 0.309679 1.1:-56428791 MS.gene52991:CDS
AGCTTTCCAACCACCAGTTT+TGG 0.319121 1.1:+56430671 None:intergenic
CAAGATTCAACCAAGATCTT+TGG 0.329664 1.1:-56428798 MS.gene52991:CDS
CCAATTGGGTTGGGACCATT+TGG 0.334713 1.1:-56429259 MS.gene52991:CDS
GCAACATAAGCACCTAATAT+AGG 0.383360 1.1:+56429230 None:intergenic
CAAGTAACAATGTCACCAAT+TGG 0.391039 1.1:-56429274 MS.gene52991:CDS
TGCATCCTTCATTTATCTCT+CGG 0.404223 1.1:-56429171 MS.gene52991:intron
CCTGTTCTTAGATCCAAAAC+TGG 0.409508 1.1:-56430684 MS.gene52991:CDS
TTGAAGGATAACTATGGAAG+AGG 0.437888 1.1:-56430763 None:intergenic
GCTATTGGCACATGTTCCCT+AGG 0.459016 1.1:-56428776 MS.gene52991:CDS
AAAGCTTGTTCCTTTGTTGT+TGG 0.460711 1.1:-56430654 MS.gene52991:intron
TACTTGCAGTCACTGTTCCT+TGG 0.461462 1.1:+56429291 None:intergenic
CCAAATGGTCCCAACCCAAT+TGG 0.463312 1.1:+56429259 None:intergenic
GTGCCAATAGCCAAAGATCT+TGG 0.477684 1.1:+56428788 None:intergenic
GTATGAAGTATTTGAAAGAA+TGG 0.490979 1.1:-56429366 MS.gene52991:CDS
GAAGCTTCTTTGTGAGGAAG+AGG 0.495772 1.1:+56429315 None:intergenic
AAGGAGGAGTACACAGAAGA+TGG 0.498352 1.1:-56430729 MS.gene52991:CDS
AGAGGGCAGAGTAGTGAAGG+AGG 0.501220 1.1:-56430745 MS.gene52991:CDS
TAATATAGGTGTCATCCAAA+TGG 0.502308 1.1:+56429244 None:intergenic
AAGTAACAATGTCACCAATT+GGG 0.513695 1.1:-56429273 MS.gene52991:CDS
TTAGATCCAAAACTGGTGGT+TGG 0.517084 1.1:-56430677 MS.gene52991:CDS
ATTTATTATACTCAGAACCT+AGG 0.517324 1.1:+56428759 None:intergenic
CTTGGTGAAGCTTCTTTGTG+AGG 0.518141 1.1:+56429309 None:intergenic
AACAATGTCACCAATTGGGT+TGG 0.525729 1.1:-56429269 MS.gene52991:CDS
ACAATGTCACCAATTGGGTT+GGG 0.534361 1.1:-56429268 MS.gene52991:CDS
TATGTTGCTGATGCTCACCT+TGG 0.550523 1.1:-56429215 MS.gene52991:CDS
TTTATTATACTCAGAACCTA+GGG 0.557093 1.1:+56428760 None:intergenic
GGAAGAGGGCAGAGTAGTGA+AGG 0.558901 1.1:-56430748 MS.gene52991:CDS
GCTTACCGAGAGATAAATGA+AGG 0.578740 1.1:+56429166 None:intergenic
GATGGAACTGTGAATCTCAA+AGG 0.591833 1.1:-56430711 MS.gene52991:CDS
TGAAGGATAACTATGGAAGA+GGG 0.606507 1.1:-56430762 None:intergenic
GTTCTTAGATCCAAAACTGG+TGG 0.657227 1.1:-56430681 MS.gene52991:CDS
CTCACAAAGAAGCTTCACCA+AGG 0.674743 1.1:-56429308 MS.gene52991:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! AAAACAAATTGATCATAAAT+TGG - chr1.1:56429417-56429436 MS.gene52991:intron 15.0%
!! TTTCTACATTTATAAATTGA+AGG + chr1.1:56430086-56430105 None:intergenic 15.0%
!! TTTCTTATAAGAAAAACAAT+AGG - chr1.1:56429818-56429837 MS.gene52991:intron 15.0%
!!! ATATATTGTAGATAACTTTT+TGG - chr1.1:56429223-56429242 MS.gene52991:CDS 15.0%
!!! CAATAATTATAATGAGTTTT+TGG + chr1.1:56430478-56430497 None:intergenic 15.0%
!!! TGAGAAAACTTATTTTTTAA+GGG - chr1.1:56429193-56429212 MS.gene52991:CDS 15.0%
!! AACACATATCAAAACAAAAA+TGG - chr1.1:56429503-56429522 MS.gene52991:intron 20.0%
!! ATGTAATTAACATGTTAGAT+GGG - chr1.1:56430698-56430717 MS.gene52991:CDS 20.0%
!! GATAGAAAATAAAATATGCA+GGG + chr1.1:56429358-56429377 None:intergenic 20.0%
!! TAAATCCTAACAAAAAGATA+TGG + chr1.1:56428900-56428919 None:intergenic 20.0%
!! TAGCTAATAATAACAATTCA+TGG + chr1.1:56428947-56428966 None:intergenic 20.0%
!! TCTTATAAGAAAATTGTGAT+TGG + chr1.1:56429810-56429829 None:intergenic 20.0%
!! TGATAGAAAATAAAATATGC+AGG + chr1.1:56429359-56429378 None:intergenic 20.0%
!!! AAGAAGAAAAGTATTATAGA+AGG - chr1.1:56429261-56429280 MS.gene52991:CDS 20.0%
!!! ACTGTTTTTTTTTTAAGGAA+AGG + chr1.1:56429329-56429348 None:intergenic 20.0%
!!! ATAGAACTTTAGAGTAAAAA+GGG - chr1.1:56429024-56429043 MS.gene52991:intron 20.0%
!!! GTGAGAAAACTTATTTTTTA+AGG - chr1.1:56429192-56429211 MS.gene52991:CDS 20.0%
!!! TAAACTTATTTTGTTTCGTT+TGG + chr1.1:56429575-56429594 None:intergenic 20.0%
!!! TATAGAACTTTAGAGTAAAA+AGG - chr1.1:56429023-56429042 MS.gene52991:intron 20.0%
! ATACTTCAACATGTATTCAT+TGG - chr1.1:56429081-56429100 MS.gene52991:intron 25.0%
! CATGTAATTAACATGTTAGA+TGG - chr1.1:56430697-56430716 MS.gene52991:CDS 25.0%
! CTAACATGTTAATTACATGT+GGG + chr1.1:56430696-56430715 None:intergenic 25.0%
! GTATTCTGTAAAAGATTGAA+GGG - chr1.1:56429046-56429065 MS.gene52991:intron 25.0%
! TCTAACATGTTAATTACATG+TGG + chr1.1:56430697-56430716 None:intergenic 25.0%
! TTCTATCATATTTGATCCAA+TGG - chr1.1:56429371-56429390 MS.gene52991:intron 25.0%
! TTTGAAAGAATGGCATATTA+TGG - chr1.1:56430164-56430183 MS.gene52991:intron 25.0%
!! AATCTCCATATCTTTTTGTT+AGG - chr1.1:56428892-56428911 MS.gene52991:intron 25.0%
!! ACATCTTTATGACCATTTTA+TGG + chr1.1:56429477-56429496 None:intergenic 25.0%
!! GTATGAAGTATTTGAAAGAA+TGG - chr1.1:56430154-56430173 MS.gene52991:intron 25.0%
!! TAATTTTAAATACACCCCAT+GGG - chr1.1:56430637-56430656 MS.gene52991:intron 25.0%
!!! ACTAATTCTGAAATTCAAAG+AGG - chr1.1:56429108-56429127 MS.gene52991:intron 25.0%
!!! ATTGAGCATTGAAATGTTTT+TGG - chr1.1:56430113-56430132 MS.gene52991:intron 25.0%
!!! CTAATTCTGAAATTCAAAGA+GGG - chr1.1:56429109-56429128 MS.gene52991:intron 25.0%
!!! GGAAGACTGTTTTTTTTTTA+AGG + chr1.1:56429334-56429353 None:intergenic 25.0%
AAGTAACAATGTCACCAATT+GGG - chr1.1:56430247-56430266 MS.gene52991:intron 30.0%
AGAAAATAAAATATGCAGGG+TGG + chr1.1:56429355-56429374 None:intergenic 30.0%
AGACAAGAAAATCTTACCAT+TGG + chr1.1:56429390-56429409 None:intergenic 30.0%
ATTTGGATGACACCTATATT+AGG - chr1.1:56430278-56430297 MS.gene52991:intron 30.0%
CATGGTGTAACAATTATTCA+TGG + chr1.1:56428929-56428948 None:intergenic 30.0%
GGTATTCTGTAAAAGATTGA+AGG - chr1.1:56429045-56429064 MS.gene52991:intron 30.0%
TCAAAAGCTTCAACAATTCT+TGG - chr1.1:56429861-56429880 MS.gene52991:intron 30.0%
TTCAGTTTGAGTCTCATAAT+AGG - chr1.1:56430514-56430533 MS.gene52991:intron 30.0%
! CTCAAACTGAATAAAGTACT+AGG + chr1.1:56430505-56430524 None:intergenic 30.0%
! GGTAAGATTTTCTTGTCTAA+TGG - chr1.1:56429392-56429411 MS.gene52991:intron 30.0%
! GTAATTTTAAATACACCCCA+TGG - chr1.1:56430636-56430655 MS.gene52991:intron 30.0%
! TAATATAGGTGTCATCCAAA+TGG + chr1.1:56430279-56430298 None:intergenic 30.0%
AGATAAGCTGTACAAAGCTA+TGG + chr1.1:56430666-56430685 None:intergenic 35.0%
AGTTTGAGTCTCATAATAGG+AGG - chr1.1:56430517-56430536 MS.gene52991:intron 35.0%
ATAAGCTGTACAAAGCTATG+GGG + chr1.1:56430664-56430683 None:intergenic 35.0%
ATGCAAGAGGATACAAACAT+GGG + chr1.1:56430603-56430622 None:intergenic 35.0%
ATGTCCACAGATTGCATTTA+AGG - chr1.1:56430549-56430568 MS.gene52991:intron 35.0%
CAAGATTCAACCAAGATCTT+TGG - chr1.1:56430722-56430741 MS.gene52991:CDS 35.0%
CAAGTAACAATGTCACCAAT+TGG - chr1.1:56430246-56430265 MS.gene52991:intron 35.0%
CACACTCGTTTACCATAAAA+TGG - chr1.1:56429462-56429481 MS.gene52991:intron 35.0%
GATAAGCTGTACAAAGCTAT+GGG + chr1.1:56430665-56430684 None:intergenic 35.0%
GCAACATAAGCACCTAATAT+AGG + chr1.1:56430293-56430312 None:intergenic 35.0%
GTTAATTACATGTGGGAGTT+GGG + chr1.1:56430689-56430708 None:intergenic 35.0%
TCAACCTTAAATGCAATCTG+TGG + chr1.1:56430556-56430575 None:intergenic 35.0%
TGCATCCTTCATTTATCTCT+CGG - chr1.1:56430349-56430368 MS.gene52991:intron 35.0%
TGTTAATTACATGTGGGAGT+TGG + chr1.1:56430690-56430709 None:intergenic 35.0%
TTCACCAATGACGAATTGTT+CGG - chr1.1:56429634-56429653 MS.gene52991:intron 35.0%
TTCCTGAGTGAAACAATTCT+TGG - chr1.1:56429673-56429692 MS.gene52991:intron 35.0%
! AAAGCTTGTTCCTTTGTTGT+TGG - chr1.1:56428866-56428885 MS.gene52991:intron 35.0%
! AGATTGAAGGGTGATAATGA+TGG - chr1.1:56429058-56429077 MS.gene52991:intron 35.0%
! GGAGATTTTACCAACAACAA+AGG + chr1.1:56428879-56428898 None:intergenic 35.0%
AACAATGTCACCAATTGGGT+TGG - chr1.1:56430251-56430270 MS.gene52991:intron 40.0%
AAGAGGATACAAACATGGGT+AGG + chr1.1:56430599-56430618 None:intergenic 40.0%
ACAATGTCACCAATTGGGTT+GGG - chr1.1:56430252-56430271 MS.gene52991:intron 40.0%
ACCAAGAAATTGAGCTCAGA+TGG - chr1.1:56429602-56429621 MS.gene52991:intron 40.0%
ACCATCTGAGCTCAATTTCT+TGG + chr1.1:56429606-56429625 None:intergenic 40.0%
ATACAAACATGGGTAGGATG+AGG + chr1.1:56430593-56430612 None:intergenic 40.0%
ATATTGCTCTCCTACAAGTG+TGG - chr1.1:56429150-56429169 MS.gene52991:intron 40.0%
CAACCAAGATCTTTGGCTAT+TGG - chr1.1:56430729-56430748 MS.gene52991:CDS 40.0%
CATGCAAGAGGATACAAACA+TGG + chr1.1:56430604-56430623 None:intergenic 40.0%
CATGTTTGTATCCTCTTGCA+TGG - chr1.1:56430602-56430621 MS.gene52991:intron 40.0%
CCTGTTCTTAGATCCAAAAC+TGG - chr1.1:56428836-56428855 MS.gene52991:intron 40.0%
GAAAAGTCCAAAAACGACCA+AGG + chr1.1:56430325-56430344 None:intergenic 40.0%
GATGGAACTGTGAATCTCAA+AGG - chr1.1:56428809-56428828 MS.gene52991:intron 40.0%
GCTTACCGAGAGATAAATGA+AGG + chr1.1:56430357-56430376 None:intergenic 40.0%
GTTCTTAGATCCAAAACTGG+TGG - chr1.1:56428839-56428858 MS.gene52991:intron 40.0%
TCAAAGAGGGAAATGAGAGA+AGG - chr1.1:56429122-56429141 MS.gene52991:intron 40.0%
TCTTCCGAACAATTCGTCAT+TGG + chr1.1:56429641-56429660 None:intergenic 40.0%
TGTATCCTCTTGCATGGTAT+TGG - chr1.1:56430608-56430627 MS.gene52991:intron 40.0%
TTAGATCCAAAACTGGTGGT+TGG - chr1.1:56428843-56428862 MS.gene52991:intron 40.0%
TTATGGTAAACGAGTGTGAC+AGG + chr1.1:56429460-56429479 None:intergenic 40.0%
! CTCTCATTTTCCACACTTGT+AGG + chr1.1:56429163-56429182 None:intergenic 40.0%
!! TACATAGCGCTGGTTGAAAA+TGG + chr1.1:56429746-56429765 None:intergenic 40.0%
!!! CCAGTTTTGGATCTAAGAAC+AGG + chr1.1:56428839-56428858 None:intergenic 40.0%
ACGAATTGTTCGGAAGAACC+TGG - chr1.1:56429644-56429663 MS.gene52991:intron 45.0%
AGATAAGGATAGTCTGGCCA+AGG + chr1.1:56429693-56429712 None:intergenic 45.0%
ATGGTAATCCAGAGTGAGTG+CGG + chr1.1:56429721-56429740 None:intergenic 45.0%
CGAATTGTTCGGAAGAACCT+GGG - chr1.1:56429645-56429664 MS.gene52991:intron 45.0%
CTCACAAAGAAGCTTCACCA+AGG - chr1.1:56430212-56430231 MS.gene52991:intron 45.0%
CTTGGTGAAGCTTCTTTGTG+AGG + chr1.1:56430214-56430233 None:intergenic 45.0%
GAAGCTTCTTTGTGAGGAAG+AGG + chr1.1:56430208-56430227 None:intergenic 45.0%
GGCCAAGAATTGTTTCACTC+AGG + chr1.1:56429678-56429697 None:intergenic 45.0%
GTCGTGAGATAAGGATAGTC+TGG + chr1.1:56429699-56429718 None:intergenic 45.0%
GTGCCAATAGCCAAAGATCT+TGG + chr1.1:56430735-56430754 None:intergenic 45.0%
TACTTGCAGTCACTGTTCCT+TGG + chr1.1:56430232-56430251 None:intergenic 45.0%
TAGACCTTTCTACATAGCGC+TGG + chr1.1:56429756-56429775 None:intergenic 45.0%
TATGTTGCTGATGCTCACCT+TGG - chr1.1:56430305-56430324 MS.gene52991:intron 45.0%
TCAACCAGCGCTATGTAGAA+AGG - chr1.1:56429749-56429768 MS.gene52991:intron 45.0%
! AAGGAGGAGTACACAGAAGA+TGG - chr1.1:56428791-56428810 MS.gene52991:CDS 45.0%
! AGCTTTCCAACCACCAGTTT+TGG + chr1.1:56428852-56428871 None:intergenic 45.0%
! ATCCTCTTGCATGGTATTGG+TGG - chr1.1:56430611-56430630 MS.gene52991:intron 45.0%
!! ATGCTCACCTTGGTCGTTTT+TGG - chr1.1:56430315-56430334 MS.gene52991:intron 45.0%
!! TCCTCTTGCATGGTATTGGT+GGG - chr1.1:56430612-56430631 MS.gene52991:intron 45.0%
ACAAAGCTATGGGGTCCCAT+GGG + chr1.1:56430655-56430674 None:intergenic 50.0%
CCAAATGGTCCCAACCCAAT+TGG + chr1.1:56430264-56430283 None:intergenic 50.0%
GCTATTGGCACATGTTCCCT+AGG - chr1.1:56430744-56430763 MS.gene52991:CDS 50.0%
GGATAGTCTGGCCAAGGATA+AGG + chr1.1:56429896-56429915 None:intergenic 50.0%
GGCCAAGGATAAGGATAGTC+TGG + chr1.1:56429887-56429906 None:intergenic 50.0%
GGCCAGACTATCCTTATCCT+TGG - chr1.1:56429882-56429901 MS.gene52991:intron 50.0%
TACAAAGCTATGGGGTCCCA+TGG + chr1.1:56430656-56430675 None:intergenic 50.0%
TCCCACCAATACCATGCAAG+AGG + chr1.1:56430616-56430635 None:intergenic 50.0%
TTCACTCAGGAATCGAACCC+AGG + chr1.1:56429665-56429684 None:intergenic 50.0%
!! CCAATTGGGTTGGGACCATT+TGG - chr1.1:56430261-56430280 MS.gene52991:intron 50.0%
!! GCGCTGGTTGAAAATGGTCA+TGG + chr1.1:56429740-56429759 None:intergenic 50.0%
AGAGGGCAGAGTAGTGAAGG+AGG - chr1.1:56428775-56428794 MS.gene52991:CDS 55.0%
CAAAGCTATGGGGTCCCATG+GGG + chr1.1:56430654-56430673 None:intergenic 55.0%
GGAAGAGGGCAGAGTAGTGA+AGG - chr1.1:56428772-56428791 MS.gene52991:CDS 55.0%
GTGAGTGCGGTCGTGAGATA+AGG + chr1.1:56429708-56429727 None:intergenic 55.0%
TCTCACGACCGCACTCACTC+TGG - chr1.1:56429710-56429729 MS.gene52991:intron 60.0%


Chromosome Type Strat End Strand Name
chr1.1 gene 56428770 56430772 56428770 ID=MS.gene52991
chr1.1 mRNA 56428770 56430772 56428770 ID=MS.gene52991.t1;Parent=MS.gene52991
chr1.1 exon 56430655 56430772 56430655 ID=MS.gene52991.t1.exon1;Parent=MS.gene52991.t1
chr1.1 CDS 56430655 56430772 56430655 ID=cds.MS.gene52991.t1;Parent=MS.gene52991.t1
chr1.1 exon 56429172 56429389 56429172 ID=MS.gene52991.t1.exon2;Parent=MS.gene52991.t1
chr1.1 CDS 56429172 56429389 56429172 ID=cds.MS.gene52991.t1;Parent=MS.gene52991.t1
chr1.1 exon 56428770 56428826 56428770 ID=MS.gene52991.t1.exon3;Parent=MS.gene52991.t1
chr1.1 CDS 56428770 56428826 56428770 ID=cds.MS.gene52991.t1;Parent=MS.gene52991.t1
Gene Sequence

>MS.gene52991

ATGGAAGAGGGCAGAGTAGTGAAGGAGGAGTACACAGAAGATGGAACTGTGAATCTCAAAGGAAAACCTGTTCTTAGATCCAAAACTGGTGGTTGGAAAGCTTGTTCCTTTGTTGTTGGTAAAATCTCCATATCTTTTTGTTAGGATTTAGTTACACCATGAATAATTGTTACACCATGAATTGTTATTATTAGCTAAAAGTGTAAAACTTTTAACATTTTTTTTATATAGTCATTTTCATCTGATTTATATATATAGAACTTTAGAGTAAAAAGGGTATTCTGTAAAAGATTGAAGGGTGATAATGATGGATACTTCAACATGTATTCATTGGTCTTACTAATTCTGAAATTCAAAGAGGGAAATGAGAGAAGGTCAAAATATTGCTCTCCTACAAGTGTGGAAAATGAGAGAAAACATGAGTGAGAAAACTTATTTTTTAAGGGATTAAAAATATATTGTAGATAACTTTTTGGTGTAAAACATTTATAAAGAAGAAAAGTATTATAGAAGGAAAAATGTTTTGTCTAACTTGTACAAGTAAGTAAGTCTTGCACCTTTCCTTAAAAAAAAAACAGTCTTCCACCCTGCATATTTTATTTTCTATCATATTTGATCCAATGGTAAGATTTTCTTGTCTAATGGTGAAAACAAATTGATCATAAATTGGTCACACATGATGTATTACCTGTCACACTCGTTTACCATAAAATGGTCATAAAGATGTGTCTTTAACACATATCAAAACAAAAATGGTATAAGACATAGTCAAACTCAACTACAATAAAAGATACATGATAAACCAAACGAAACAAAATAAGTTTAAATGTTAACCAAGAAATTGAGCTCAGATGGTAAATAAGTTTCACCAATGACGAATTGTTCGGAAGAACCTGGGTTCGATTCCTGAGTGAAACAATTCTTGGCCAGACTATCCTTATCTCACGACCGCACTCACTCTGGATTACCATGACCATTTTCAACCAGCGCTATGTAGAAAGGTCTATAACTCTATTTCTACTACTAACAAATCATCTCCAATCACAATTTTCTTATAAGAAAAACAATAGGTTCATGTCTACATCAACACTTCAAAAGCTTCAACAATTCTTGGCCAGACTATCCTTATCCTTGGCCAGACTATCCTTATCTCACGACCGCACTCACTCTGGATTACCATGACCATTTTCAACCAGCGCTATGTAGAAAGGTCTATAACTCTATTTCTACTACTAACAAATCATCTCCAATCACAATTTTCTTATAAGAAAAACAATAGGTTCATGTCTACATCAACACTTCAAAAGCTTCAACCTTCAATTTATAAATGTAGAAACTTTATTATTGAGCATTGAAATGTTTTTGGTTGAATGTATGTTGCAGTGTATGAAGTATTTGAAAGAATGGCATATTATGGAATATCATCGAATTTGATCCTCTTCCTCACAAAGAAGCTTCACCAAGGAACAGTGACTGCAAGTAACAATGTCACCAATTGGGTTGGGACCATTTGGATGACACCTATATTAGGTGCTTATGTTGCTGATGCTCACCTTGGTCGTTTTTGGACTTTTCTCATTGCATCCTTCATTTATCTCTCGGTAAGCAATAGTATGCTATAACTTTATTTATAAGCAAAGTCTTAATCACAAGAGTTCAATTTCAATTAAAGTAGTATTTTTAATTTAAAACATACAATCATTGTCCAAAAACTCATTATAATTATTGTAGTCCTAGTACTTTATTCAGTTTGAGTCTCATAATAGGAGGACATCTAACATGTCCACAGATTGCATTTAAGGTTGATAATTTAATTGAAACCTCATCCTACCCATGTTTGTATCCTCTTGCATGGTATTGGTGGGAGTAATTTTAAATACACCCCATGGGACCCCATAGCTTTGTACAGCTTATCTCCCAACTCCCACATGTAATTAACATGTTAGATGGGTCAAGATTCAACCAAGATCTTTGGCTATTGGCACATGTTCCCTAGGTTCTGA
Protein sequence

>MS.gene52991.t1

MEEGRVVKEEYTEDGTVNLKGKPVLRSKTGGWKACSFVVVYEVFERMAYYGISSNLILFLTKKLHQGTVTASNNVTNWVGTIWMTPILGAYVADAHLGRFWTFLIASFIYLSMGQDSTKIFGYWHMFPRF