AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene057750


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene057750.t1 MTR_4g076940 98.837 86 1 0 1 86 1 86 2.10e-53 176
MS.gene057750.t1 MTR_4g076940 98.837 86 1 0 1 86 41 126 2.92e-53 176
MS.gene057750.t1 MTR_4g076900 98.148 54 1 0 33 86 9 62 1.80e-32 118
MS.gene057750.t1 MTR_4g076970 62.069 87 29 2 1 86 1 84 5.21e-26 100
MS.gene057750.t1 MTR_4g094060 57.895 57 24 0 30 86 50 106 3.54e-16 72.0
MS.gene057750.t1 MTR_4g093845 64.815 54 17 1 33 86 41 92 3.93e-16 71.6
MS.gene057750.t1 MTR_4g094050 57.895 57 24 0 30 86 50 106 4.39e-16 71.6
MS.gene057750.t1 MTR_7g091350 57.407 54 20 1 32 85 2 52 3.72e-15 66.2
MS.gene057750.t1 MTR_4g053980 53.448 58 27 0 30 87 46 103 8.16e-15 64.3
MS.gene057750.t1 MTR_4g094090 52.459 61 27 2 26 86 6 64 2.01e-13 63.9
MS.gene057750.t1 MTR_6g066240 54.717 53 23 1 33 85 47 98 1.18e-12 62.0
MS.gene057750.t1 MTR_4g094010 42.045 88 42 3 1 86 3 83 3.15e-12 60.8
MS.gene057750.t1 MTR_1g108340 48.148 54 28 0 33 86 73 126 8.64e-11 56.6
MS.gene057750.t1 MTR_1g108340 48.148 54 28 0 33 86 73 126 8.65e-11 56.6
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene057750.t1 AT1G51500 78.333 60 13 0 27 86 18 77 1.27e-28 107
MS.gene057750.t1 AT3G21090 76.667 60 14 0 27 86 19 78 4.50e-28 105
MS.gene057750.t1 AT1G51460 76.364 55 13 0 32 86 9 63 4.58e-23 91.7
MS.gene057750.t1 AT1G17840 60.377 53 20 1 33 85 50 101 1.82e-15 70.1

Find 21 sgRNAs with CRISPR-Local

Find 26 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
AGAGTTATGTTGCCAAATTT+TGG 0.179448 4.4:+36253497 MS.gene057750:CDS
GTTGGTCCTTTCCCAAAATT+TGG 0.179855 4.4:-36253509 None:intergenic
AGGTTACTTAATGGCCTTAA+TGG 0.232273 4.4:+36253536 MS.gene057750:CDS
TGGTAGAATCATGGCTATTA+TGG 0.271739 4.4:+36253571 MS.gene057750:CDS
TTGGGAAAGGACCAACTAAA+AGG 0.304543 4.4:+36253516 MS.gene057750:CDS
CCAACTAAAAGGTTACTTAA+TGG 0.346671 4.4:+36253527 MS.gene057750:CDS
ACTTTGCTTGATACACTTGC+AGG 0.353311 4.4:+36253617 MS.gene057750:CDS
CAGGCTCAGCAAAACCATTA+AGG 0.385540 4.4:-36253550 None:intergenic
ATGGCTATTATGGGTCCTTC+TGG 0.416172 4.4:+36253581 MS.gene057750:CDS
ATTATGGGTCCTTCTGGCTC+TGG 0.429652 4.4:+36253587 MS.gene057750:CDS
GGTAGAATCATGGCTATTAT+GGG 0.431697 4.4:+36253572 MS.gene057750:CDS
TGCTGAGCCTGGTAGAATCA+TGG 0.476639 4.4:+36253562 MS.gene057750:CDS
GCATATTGTGGTCTTAATAG+AGG 0.502246 4.4:+36253449 MS.gene057750:CDS
ATAGAGGAAGCTTCTTAGCA+TGG 0.554454 4.4:+36253465 MS.gene057750:CDS
TAGAGGAAGCTTCTTAGCAT+GGG 0.556492 4.4:+36253466 MS.gene057750:CDS
TGCAAGTGTATCAAGCAAAG+TGG 0.586411 4.4:-36253615 None:intergenic
ATAATAGCCATGATTCTACC+AGG 0.623399 4.4:-36253569 None:intergenic
CAACAACAAAGGATGCAACA+TGG 0.630369 4.4:+36253409 MS.gene057750:CDS
ATGGAGATTGAAGCAGCAAG+TGG 0.659585 4.4:+36253383 MS.gene057750:CDS
GCAAGTGGTAGCAACAACAA+AGG 0.683774 4.4:+36253398 MS.gene057750:CDS
AGTGATGAAGAAGCATATTG+TGG 0.688694 4.4:+36253437 MS.gene057750:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
CCAACTAAAAGGTTACTTAA+TGG + chr4.4:36253527-36253546 MS.gene057750:CDS 30.0%
! AGAGTTATGTTGCCAAATTT+TGG + chr4.4:36253497-36253516 MS.gene057750:CDS 30.0%
! CCATTAAGTAACCTTTTAGT+TGG - chr4.4:36253530-36253549 None:intergenic 30.0%
! GAGTTATGTTGCCAAATTTT+GGG + chr4.4:36253498-36253517 MS.gene057750:CDS 30.0%
ATAATAGCCATGATTCTACC+AGG - chr4.4:36253572-36253591 None:intergenic 35.0%
GGTAGAATCATGGCTATTAT+GGG + chr4.4:36253572-36253591 MS.gene057750:CDS 35.0%
TGGTAGAATCATGGCTATTA+TGG + chr4.4:36253571-36253590 MS.gene057750:CDS 35.0%
! AGGTTACTTAATGGCCTTAA+TGG + chr4.4:36253536-36253555 MS.gene057750:CDS 35.0%
! AGTGATGAAGAAGCATATTG+TGG + chr4.4:36253437-36253456 MS.gene057750:CDS 35.0%
! GCATATTGTGGTCTTAATAG+AGG + chr4.4:36253449-36253468 MS.gene057750:CDS 35.0%
!! ATGTTGCCAAATTTTGGGAA+AGG + chr4.4:36253503-36253522 MS.gene057750:CDS 35.0%
ACTTTGCTTGATACACTTGC+AGG + chr4.4:36253617-36253636 MS.gene057750:CDS 40.0%
ATAGAGGAAGCTTCTTAGCA+TGG + chr4.4:36253465-36253484 MS.gene057750:CDS 40.0%
CAACAACAAAGGATGCAACA+TGG + chr4.4:36253409-36253428 MS.gene057750:CDS 40.0%
GTTGGTCCTTTCCCAAAATT+TGG - chr4.4:36253512-36253531 None:intergenic 40.0%
TAGAGGAAGCTTCTTAGCAT+GGG + chr4.4:36253466-36253485 MS.gene057750:CDS 40.0%
TGCAAGTGTATCAAGCAAAG+TGG - chr4.4:36253618-36253637 None:intergenic 40.0%
TTGGGAAAGGACCAACTAAA+AGG + chr4.4:36253516-36253535 MS.gene057750:CDS 40.0%
ATGGAGATTGAAGCAGCAAG+TGG + chr4.4:36253383-36253402 MS.gene057750:CDS 45.0%
ATGGCTATTATGGGTCCTTC+TGG + chr4.4:36253581-36253600 MS.gene057750:CDS 45.0%
CAGGCTCAGCAAAACCATTA+AGG - chr4.4:36253553-36253572 None:intergenic 45.0%
GCAAGTGGTAGCAACAACAA+AGG + chr4.4:36253398-36253417 MS.gene057750:CDS 45.0%
!! CTTAATGGTTTTGCTGAGCC+TGG + chr4.4:36253551-36253570 MS.gene057750:CDS 45.0%
TGCTGAGCCTGGTAGAATCA+TGG + chr4.4:36253562-36253581 MS.gene057750:CDS 50.0%
! GTGGATTTTCCAGAGCCAGA+AGG - chr4.4:36253599-36253618 None:intergenic 50.0%
!! ATTATGGGTCCTTCTGGCTC+TGG + chr4.4:36253587-36253606 MS.gene057750:CDS 50.0%


Chromosome Type Strat End Strand Name
chr4.4 gene 36253383 36253646 36253383 ID=MS.gene057750
chr4.4 mRNA 36253383 36253646 36253383 ID=MS.gene057750.t1;Parent=MS.gene057750
chr4.4 exon 36253383 36253646 36253383 ID=MS.gene057750.t1.exon1;Parent=MS.gene057750.t1
chr4.4 CDS 36253383 36253646 36253383 ID=cds.MS.gene057750.t1;Parent=MS.gene057750.t1
Gene Sequence

>MS.gene057750

ATGGAGATTGAAGCAGCAAGTGGTAGCAACAACAAAGGATGCAACATGGATGAGAGTGATGAAGAAGCATATTGTGGTCTTAATAGAGGAAGCTTCTTAGCATGGGAAGATCTAAGAGTTATGTTGCCAAATTTTGGGAAAGGACCAACTAAAAGGTTACTTAATGGCCTTAATGGTTTTGCTGAGCCTGGTAGAATCATGGCTATTATGGGTCCTTCTGGCTCTGGAAAATCCACTTTGCTTGATACACTTGCAGGTTCATAA

Protein sequence

>MS.gene057750.t1

MEIEAASGSNNKGCNMDESDEEAYCGLNRGSFLAWEDLRVMLPNFGKGPTKRLLNGLNGFAEPGRIMAIMGPSGSGKSTLLDTLAGS