Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene53234.t1 | XP_003598034.1 | 95.3 | 150 | 7 | 0 | 1 | 150 | 1 | 150 | 2.30E-74 | 288.1 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene53234.t1 | P56331 | 80.8 | 151 | 22 | 3 | 1 | 150 | 1 | 145 | 2.4e-59 | 229.6 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene53234.t1 | G7IYF3 | 95.3 | 150 | 7 | 0 | 1 | 150 | 1 | 150 | 1.7e-74 | 288.1 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene057208 | MS.gene53234 | 0.811124 | 8.19E-51 | -1.69E-46 |
| MS.gene058769 | MS.gene53234 | 0.863682 | 2.09E-64 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene53234.t1 | MTR_3g005520 | 95.333 | 150 | 7 | 0 | 1 | 150 | 1 | 150 | 5.66e-90 | 258 |
| MS.gene53234.t1 | MTR_8g028180 | 84.000 | 150 | 20 | 1 | 1 | 150 | 1 | 146 | 1.75e-74 | 219 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene53234.t1 | AT2G04520 | 82.000 | 150 | 22 | 2 | 1 | 150 | 1 | 145 | 3.55e-77 | 226 |
| MS.gene53234.t1 | AT5G35680 | 80.000 | 150 | 25 | 2 | 1 | 150 | 1 | 145 | 2.75e-71 | 211 |
| MS.gene53234.t1 | AT5G35680 | 80.000 | 150 | 25 | 2 | 1 | 150 | 1 | 145 | 2.75e-71 | 211 |
| MS.gene53234.t1 | AT5G35680 | 86.179 | 123 | 13 | 1 | 1 | 123 | 1 | 119 | 1.58e-69 | 207 |
Find 35 sgRNAs with CRISPR-Local
Find 36 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| TGTTGGATTACGTGATTATC+AGG | 0.304283 | 3.3:+888062 | MS.gene53234:CDS |
| ACGAGCTTCCGGAATCTATT+AGG | 0.305997 | 3.3:+888148 | MS.gene53234:CDS |
| AACGGAGATATCATTCTTGT+TGG | 0.394753 | 3.3:+888045 | MS.gene53234:CDS |
| GAAGGCTTATGACGAGCTTC+CGG | 0.397993 | 3.3:+888137 | MS.gene53234:CDS |
| TTGAGGATGAGGATATTGAT+AGG | 0.400368 | 3.3:+888241 | MS.gene53234:CDS |
| GTAAGATGCATAAGAAGGTT+TGG | 0.408295 | 3.3:+888016 | MS.gene53234:CDS |
| TTCAACAATCTTGCCTCATC+CGG | 0.420919 | 3.3:-888117 | None:intergenic |
| AGGCTTAATGAAGGGATTGC+TGG | 0.437830 | 3.3:+888168 | MS.gene53234:CDS |
| GCGTCCGTTTCCGAGCATAT+TGG | 0.444772 | 3.3:-887932 | None:intergenic |
| TCATTAAGCCTAATAGATTC+CGG | 0.444954 | 3.3:-888156 | None:intergenic |
| ATGCCGAAGAACAAGGGAAA+AGG | 0.492369 | 3.3:+887817 | MS.gene53234:CDS |
| GCTCGGAAACGGACGCTTGG+AGG | 0.497540 | 3.3:+887939 | MS.gene53234:CDS |
| GGATGAGGCAAGATTGTTGA+AGG | 0.502559 | 3.3:+888119 | MS.gene53234:CDS |
| TGAAGGGATTGCTGGTGGGA+TGG | 0.507121 | 3.3:+888176 | MS.gene53234:CDS |
| ACGTGATTATCAGGATGATA+AGG | 0.510200 | 3.3:+888071 | MS.gene53234:CDS |
| TATGCTCGGAAACGGACGCT+TGG | 0.513890 | 3.3:+887936 | MS.gene53234:CDS |
| GAATCTATTAGGCTTAATGA+AGG | 0.530788 | 3.3:+888159 | MS.gene53234:CDS |
| ATTGAAATATATGCCGGATG+AGG | 0.546058 | 3.3:+888104 | MS.gene53234:CDS |
| ATCAACCATGCCGAAGAACA+AGG | 0.554015 | 3.3:+887810 | None:intergenic |
| GTAACCAATATGCTCGGAAA+CGG | 0.559215 | 3.3:+887928 | MS.gene53234:CDS |
| CCGCGGTAAGATGCATAAGA+AGG | 0.576217 | 3.3:+888011 | MS.gene53234:CDS |
| AAGAAGGTTTGGATTGCTAA+CGG | 0.592201 | 3.3:+888027 | MS.gene53234:CDS |
| CCGAAGAACAAGGGAAAAGG+AGG | 0.596700 | 3.3:+887820 | MS.gene53234:CDS |
| CTTAATGAAGGGATTGCTGG+TGG | 0.602290 | 3.3:+888171 | MS.gene53234:CDS |
| AATCTATTAGGCTTAATGAA+GGG | 0.608999 | 3.3:+888160 | MS.gene53234:CDS |
| ATGGATGATCATGATGATGA+TGG | 0.612732 | 3.3:+888195 | MS.gene53234:CDS |
| TGTCATATTGAAATATATGC+CGG | 0.619355 | 3.3:+888098 | MS.gene53234:CDS |
| TCAACCATGCCGAAGAACAA+GGG | 0.623500 | 3.3:+887811 | None:intergenic |
| GGAGGAAAGAATCGAAAGCG+AGG | 0.627611 | 3.3:+887838 | MS.gene53234:CDS |
| CCTTCTTATGCATCTTACCG+CGG | 0.648150 | 3.3:-888011 | None:intergenic |
| GAACTCATCTTCAAAGAAGA+CGG | 0.648957 | 3.3:+887889 | MS.gene53234:CDS |
| GCACAGGTAACCAATATGCT+CGG | 0.649194 | 3.3:+887922 | MS.gene53234:CDS |
| TTAATGAAGGGATTGCTGGT+GGG | 0.657015 | 3.3:+888172 | MS.gene53234:CDS |
| AGACGGTCAAGAATACGCAC+AGG | 0.669279 | 3.3:+887906 | MS.gene53234:CDS |
| AAACGTCTCTGTCATATCCG+CGG | 0.693679 | 3.3:+887994 | MS.gene53234:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !!! | TAATGATTATGTTCATTTTG+AGG | + | chr3.3:888224-888243 | MS.gene53234:CDS | 20.0% |
| ! | AATCTATTAGGCTTAATGAA+GGG | + | chr3.3:888160-888179 | MS.gene53234:CDS | 25.0% |
| ! | TGTCATATTGAAATATATGC+CGG | + | chr3.3:888098-888117 | MS.gene53234:CDS | 25.0% |
| GAATCTATTAGGCTTAATGA+AGG | + | chr3.3:888159-888178 | MS.gene53234:CDS | 30.0% | |
| TCATTAAGCCTAATAGATTC+CGG | - | chr3.3:888159-888178 | None:intergenic | 30.0% | |
| !! | TTATGTTCATTTTGAGGATG+AGG | + | chr3.3:888230-888249 | MS.gene53234:CDS | 30.0% |
| AACGGAGATATCATTCTTGT+TGG | + | chr3.3:888045-888064 | MS.gene53234:CDS | 35.0% | |
| AAGAAGGTTTGGATTGCTAA+CGG | + | chr3.3:888027-888046 | MS.gene53234:CDS | 35.0% | |
| ACGTGATTATCAGGATGATA+AGG | + | chr3.3:888071-888090 | MS.gene53234:CDS | 35.0% | |
| ATTGAAATATATGCCGGATG+AGG | + | chr3.3:888104-888123 | MS.gene53234:CDS | 35.0% | |
| GAACTCATCTTCAAAGAAGA+CGG | + | chr3.3:887889-887908 | MS.gene53234:CDS | 35.0% | |
| GTAAGATGCATAAGAAGGTT+TGG | + | chr3.3:888016-888035 | MS.gene53234:CDS | 35.0% | |
| TGTTGGATTACGTGATTATC+AGG | + | chr3.3:888062-888081 | MS.gene53234:CDS | 35.0% | |
| TTGAGGATGAGGATATTGAT+AGG | + | chr3.3:888241-888260 | MS.gene53234:CDS | 35.0% | |
| ! | ATGGATGATCATGATGATGA+TGG | + | chr3.3:888195-888214 | MS.gene53234:CDS | 35.0% |
| GTAACCAATATGCTCGGAAA+CGG | + | chr3.3:887928-887947 | MS.gene53234:CDS | 40.0% | |
| TTAATGAAGGGATTGCTGGT+GGG | + | chr3.3:888172-888191 | MS.gene53234:CDS | 40.0% | |
| TTCAACAATCTTGCCTCATC+CGG | - | chr3.3:888120-888139 | None:intergenic | 40.0% | |
| AAACGTCTCTGTCATATCCG+CGG | + | chr3.3:887994-888013 | MS.gene53234:CDS | 45.0% | |
| ACGAGCTTCCGGAATCTATT+AGG | + | chr3.3:888148-888167 | MS.gene53234:CDS | 45.0% | |
| AGGCTTAATGAAGGGATTGC+TGG | + | chr3.3:888168-888187 | MS.gene53234:CDS | 45.0% | |
| ATGCCGAAGAACAAGGGAAA+AGG | + | chr3.3:887817-887836 | MS.gene53234:CDS | 45.0% | |
| CCTTCTTATGCATCTTACCG+CGG | - | chr3.3:888014-888033 | None:intergenic | 45.0% | |
| CTTAATGAAGGGATTGCTGG+TGG | + | chr3.3:888171-888190 | MS.gene53234:CDS | 45.0% | |
| GCACAGGTAACCAATATGCT+CGG | + | chr3.3:887922-887941 | MS.gene53234:CDS | 45.0% | |
| GGATGAGGCAAGATTGTTGA+AGG | + | chr3.3:888119-888138 | MS.gene53234:CDS | 45.0% | |
| ! | CCTCCTTTTCCCTTGTTCTT+CGG | - | chr3.3:887823-887842 | None:intergenic | 45.0% |
| AGACGGTCAAGAATACGCAC+AGG | + | chr3.3:887906-887925 | MS.gene53234:CDS | 50.0% | |
| CCGAAGAACAAGGGAAAAGG+AGG | + | chr3.3:887820-887839 | MS.gene53234:CDS | 50.0% | |
| CCGCGGTAAGATGCATAAGA+AGG | + | chr3.3:888011-888030 | MS.gene53234:CDS | 50.0% | |
| GAAGGCTTATGACGAGCTTC+CGG | + | chr3.3:888137-888156 | MS.gene53234:CDS | 50.0% | |
| GGAGGAAAGAATCGAAAGCG+AGG | + | chr3.3:887838-887857 | MS.gene53234:CDS | 50.0% | |
| GCGTCCGTTTCCGAGCATAT+TGG | - | chr3.3:887935-887954 | None:intergenic | 55.0% | |
| TATGCTCGGAAACGGACGCT+TGG | + | chr3.3:887936-887955 | MS.gene53234:CDS | 55.0% | |
| TGAAGGGATTGCTGGTGGGA+TGG | + | chr3.3:888176-888195 | MS.gene53234:CDS | 55.0% | |
| GCTCGGAAACGGACGCTTGG+AGG | + | chr3.3:887939-887958 | MS.gene53234:CDS | 65.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr3.3 | gene | 887817 | 888269 | 887817 | ID=MS.gene53234 |
| chr3.3 | mRNA | 887817 | 888269 | 887817 | ID=MS.gene53234.t1;Parent=MS.gene53234 |
| chr3.3 | exon | 887817 | 888269 | 887817 | ID=MS.gene53234.t1.exon1;Parent=MS.gene53234.t1 |
| chr3.3 | CDS | 887817 | 888269 | 887817 | ID=cds.MS.gene53234.t1;Parent=MS.gene53234.t1 |
| Gene Sequence |
| Protein sequence |
