AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene53239

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene53239.t1 MTR_3g005560 94.582 646 9 1 1 620 49 694 0.0 1256
MS.gene53239.t1 MTR_3g450800 72.558 645 150 2 1 620 1 643 0.0 996
MS.gene53239.t1 MTR_2g037830 71.207 646 158 2 1 620 49 692 0.0 970
MS.gene53239.t1 MTR_0011s0020 69.659 646 168 2 1 620 49 692 0.0 950
MS.gene53239.t1 MTR_3g005560 93.469 490 6 1 1 464 49 538 0.0 944
MS.gene53239.t1 MTR_3g005560 91.957 373 4 1 1 347 49 421 0.0 705
MS.gene53239.t1 MTR_2g037830 74.588 425 82 1 1 399 49 473 0.0 664
MS.gene53239.t1 MTR_1g073740 40.916 655 338 8 4 618 66 711 0.0 550
MS.gene53239.t1 MTR_4g088225 41.960 653 336 6 4 618 65 712 0.0 548
MS.gene53239.t1 MTR_4g088225 41.960 653 336 6 4 618 65 712 0.0 547
MS.gene53239.t1 MTR_3g060220 34.436 665 370 11 4 618 58 706 6.73e-130 399
MS.gene53239.t1 MTR_3g060220 34.384 666 370 11 4 618 58 707 9.14e-130 398
MS.gene53239.t1 MTR_3g060240 33.483 669 375 11 4 618 58 710 6.13e-127 391
MS.gene53239.t1 MTR_3g060280 33.284 670 371 13 1 616 65 712 3.05e-123 382
MS.gene53239.t1 MTR_8g063270 31.356 708 385 16 5 619 36 735 1.07e-108 345
MS.gene53239.t1 MTR_8g063270 31.356 708 385 16 5 619 241 940 1.63e-106 344
MS.gene53239.t1 MTR_4g130510 28.631 723 400 14 3 619 285 997 2.79e-102 334
MS.gene53239.t1 MTR_3g060280 36.257 513 260 9 1 465 65 558 1.46e-100 318
MS.gene53239.t1 MTR_2g087900 30.963 675 393 18 3 612 56 722 9.62e-100 321
MS.gene53239.t1 MTR_3g060280 35.938 512 261 9 1 464 65 557 1.19e-99 316
MS.gene53239.t1 MTR_4g050000 29.895 669 398 16 3 612 54 710 1.97e-97 314
MS.gene53239.t1 MTR_8g086600 28.198 727 404 13 3 619 230 948 6.09e-97 318
MS.gene53239.t1 MTR_2g087960 30.832 613 364 11 12 569 66 673 6.21e-97 313
MS.gene53239.t1 MTR_1g069605 28.552 718 403 14 2 619 144 851 6.88e-97 316
MS.gene53239.t1 MTR_4g130510 27.477 757 399 17 3 619 285 1031 6.29e-96 317
MS.gene53239.t1 MTR_2g016630 27.633 731 406 13 3 619 215 936 7.26e-95 313
MS.gene53239.t1 MTR_2g087980 30.218 642 398 11 19 612 68 707 8.48e-94 305
MS.gene53239.t1 MTR_2g087950 30.120 664 397 14 3 612 54 704 4.93e-93 303
MS.gene53239.t1 MTR_2g087900 33.269 520 285 10 3 463 56 572 1.15e-89 290
MS.gene53239.t1 MTR_4g130510 30.809 581 290 12 3 480 285 856 1.73e-89 296
MS.gene53239.t1 MTR_3g007770 27.979 747 398 18 3 619 272 1008 2.92e-89 299
MS.gene53239.t1 MTR_2g087960 32.400 500 281 9 19 464 73 569 2.88e-87 283
MS.gene53239.t1 MTR_3g060280 36.364 407 189 10 1 356 65 452 7.52e-74 244
MS.gene53239.t1 MTR_2g087900 34.442 421 214 10 3 365 56 472 2.84e-72 241
MS.gene53239.t1 MTR_7g106680 35.329 334 160 5 2 279 262 595 1.92e-64 229
MS.gene53239.t1 MTR_7g106680 27.385 325 224 6 298 618 674 990 1.26e-37 150
MS.gene53239.t1 MTR_4g130510 30.717 446 207 8 3 348 285 728 6.15e-63 221
MS.gene53239.t1 MTR_8g092590 34.985 343 166 6 2 287 322 664 1.14e-61 222
MS.gene53239.t1 MTR_8g092590 27.988 343 232 8 282 618 721 1054 1.25e-38 153
MS.gene53239.t1 MTR_3g030040 35.435 333 159 6 3 279 305 637 1.70e-61 221
MS.gene53239.t1 MTR_3g030040 27.356 329 227 6 295 619 717 1037 5.53e-39 154
MS.gene53239.t1 MTR_8g037260 34.615 338 165 5 3 284 305 642 1.87e-61 221
MS.gene53239.t1 MTR_8g037260 27.492 331 228 6 293 619 715 1037 1.41e-40 159
MS.gene53239.t1 MTR_2g087900 37.500 288 137 5 3 247 56 343 3.28e-61 207
MS.gene53239.t1 MTR_1g098550 35.569 343 164 6 2 287 324 666 9.69e-61 219
MS.gene53239.t1 MTR_1g098550 28.319 339 204 8 292 613 734 1050 5.47e-33 135
MS.gene53239.t1 MTR_1g098550 35.569 343 164 6 2 287 320 662 1.03e-60 219
MS.gene53239.t1 MTR_1g098550 28.319 339 204 8 292 613 730 1046 5.40e-33 135
MS.gene53239.t1 MTR_3g107520 33.903 351 164 6 3 291 315 659 4.20e-59 214
MS.gene53239.t1 MTR_3g107520 27.273 330 226 8 295 619 725 1045 6.13e-35 142
MS.gene53239.t1 MTR_2g035780 33.533 334 166 5 2 279 255 588 8.40e-59 213
MS.gene53239.t1 MTR_2g035780 26.748 329 228 7 295 619 684 1003 2.03e-35 143
MS.gene53239.t1 MTR_5g029190 24.809 524 329 9 155 619 566 1083 1.14e-52 195
MS.gene53239.t1 MTR_5g029190 40.157 127 64 3 3 117 302 428 3.08e-22 102
MS.gene53239.t1 MTR_4g077910 25.872 545 325 10 141 619 573 1104 3.76e-52 194
MS.gene53239.t1 MTR_4g077910 41.732 127 62 2 3 117 323 449 4.18e-22 102
MS.gene53239.t1 MTR_2g438220 25.954 524 316 14 155 618 474 985 9.10e-52 192
MS.gene53239.t1 MTR_2g438220 34.641 153 84 4 3 143 209 357 5.51e-21 98.2
MS.gene53239.t1 MTR_1g039480 25.368 544 322 10 147 619 563 1093 1.11e-49 186
MS.gene53239.t1 MTR_1g039480 38.710 124 64 1 5 116 311 434 2.26e-21 99.8
MS.gene53239.t1 MTR_1g039445 25.472 530 325 11 147 619 549 1065 4.45e-49 184
MS.gene53239.t1 MTR_1g039445 41.129 124 61 3 5 116 297 420 2.48e-21 99.8
MS.gene53239.t1 MTR_3g091270 27.143 420 188 11 38 369 107 496 2.23e-37 145
MS.gene53239.t1 MTR_7g116230 29.861 288 147 4 141 374 394 680 2.39e-33 136
MS.gene53239.t1 MTR_7g116230 41.525 118 57 2 12 117 188 305 2.81e-20 95.9
MS.gene53239.t1 MTR_2g087900 43.590 117 63 1 3 116 56 172 7.19e-29 117
MS.gene53239.t1 MTR_7g109200 36.932 176 98 4 126 300 587 750 1.11e-27 119
MS.gene53239.t1 MTR_7g109200 38.281 128 67 2 5 120 349 476 1.75e-21 100
MS.gene53239.t1 MTR_7g109200 23.667 300 208 6 329 618 843 1131 1.75e-20 97.1
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene53239.t1 AT4G24000 34.709 654 366 9 1 613 57 690 1.60e-141 428
MS.gene53239.t1 AT4G24010 35.090 664 363 12 1 616 60 703 3.37e-140 426
MS.gene53239.t1 AT4G23990 35.464 657 356 11 5 613 83 719 5.68e-140 425
MS.gene53239.t1 AT1G55850 33.683 668 363 13 1 619 69 705 1.42e-119 372
MS.gene53239.t1 AT5G17420 28.966 725 398 15 2 619 271 985 3.12e-106 344
MS.gene53239.t1 AT4G24000 35.579 475 283 6 142 613 44 498 1.06e-105 330
MS.gene53239.t1 AT4G23990 35.685 482 280 7 142 613 44 505 6.30e-103 323
MS.gene53239.t1 AT4G18780 28.668 736 395 14 3 619 218 942 1.09e-100 328
MS.gene53239.t1 AT2G21770 28.320 738 401 16 2 618 315 1045 6.16e-99 326
MS.gene53239.t1 AT2G32530 32.562 648 351 19 3 577 56 690 8.53e-99 318
MS.gene53239.t1 AT4G32410 27.544 737 408 13 3 619 309 1039 1.81e-98 324
MS.gene53239.t1 AT2G25540 26.882 744 406 15 2 619 288 1019 7.86e-97 320
MS.gene53239.t1 AT2G32540 31.231 650 361 16 3 579 56 692 5.34e-93 303
MS.gene53239.t1 AT2G32620 31.963 657 352 19 3 581 56 695 2.39e-92 301
MS.gene53239.t1 AT4G15320 31.087 653 363 17 3 582 56 694 2.97e-91 298
MS.gene53239.t1 AT4G15320 31.087 653 363 17 3 582 56 694 3.13e-91 298
MS.gene53239.t1 AT4G15290 30.308 650 371 16 3 582 56 693 2.08e-89 293
MS.gene53239.t1 AT4G15320 30.389 668 363 17 3 582 56 709 6.65e-89 292
MS.gene53239.t1 AT2G32610 31.062 631 339 16 19 571 72 684 2.91e-88 290
MS.gene53239.t1 AT2G32620 32.248 614 326 17 43 581 1 599 6.31e-87 285
MS.gene53239.t1 AT2G32620 34.286 525 267 14 3 463 56 566 1.86e-86 282
MS.gene53239.t1 AT2G32620 35.421 463 223 12 3 402 56 505 6.65e-82 268
MS.gene53239.t1 AT4G15320 29.228 674 341 18 3 568 56 701 7.65e-76 258
MS.gene53239.t1 AT5G64740 26.969 749 400 20 2 618 309 1042 2.12e-69 244
MS.gene53239.t1 AT5G09870 36.443 343 161 6 2 287 297 639 2.49e-64 229
MS.gene53239.t1 AT5G09870 27.893 337 218 8 292 618 706 1027 1.13e-36 147
MS.gene53239.t1 AT5G05170 35.435 333 159 5 3 279 293 625 6.92e-62 222
MS.gene53239.t1 AT5G05170 26.536 358 242 8 266 619 684 1024 5.96e-38 151
MS.gene53239.t1 AT4G39350 34.731 334 162 6 2 279 310 643 1.01e-59 216
MS.gene53239.t1 AT4G39350 26.946 334 225 6 292 618 720 1041 1.66e-35 144
MS.gene53239.t1 AT5G44030 34.431 334 163 6 2 279 248 581 1.47e-58 213
MS.gene53239.t1 AT5G44030 26.957 345 220 10 291 619 680 1008 3.56e-32 133
MS.gene53239.t1 AT5G44030 34.431 334 163 6 2 279 277 610 1.50e-58 213
MS.gene53239.t1 AT5G44030 26.957 345 220 10 291 619 709 1037 4.63e-32 133
MS.gene53239.t1 AT5G44030 36.455 299 141 5 30 279 4 302 1.29e-55 202
MS.gene53239.t1 AT5G44030 26.957 345 220 10 291 619 401 729 2.40e-32 133
MS.gene53239.t1 AT5G16910 26.119 536 320 10 147 619 585 1107 7.82e-54 199
MS.gene53239.t1 AT5G16910 37.302 126 67 1 3 116 331 456 1.40e-21 100
MS.gene53239.t1 AT3G03050 25.417 539 323 9 147 619 582 1107 2.55e-52 194
MS.gene53239.t1 AT3G03050 40.476 126 63 3 3 116 328 453 7.94e-22 101
MS.gene53239.t1 AT4G38190 25.862 522 325 10 147 619 560 1068 3.84e-51 191
MS.gene53239.t1 AT4G38190 39.683 126 64 2 3 116 306 431 4.67e-22 102
MS.gene53239.t1 AT2G33100 23.841 604 379 12 79 618 413 999 3.92e-51 191
MS.gene53239.t1 AT2G33100 35.294 153 83 4 3 143 217 365 1.16e-20 97.4
MS.gene53239.t1 AT2G33100 23.841 604 379 12 79 618 413 999 3.92e-51 191
MS.gene53239.t1 AT2G33100 35.294 153 83 4 3 143 217 365 1.16e-20 97.4
MS.gene53239.t1 AT1G02730 24.457 552 331 10 141 618 604 1143 4.81e-49 185
MS.gene53239.t1 AT1G02730 37.405 131 70 2 2 120 351 481 1.25e-21 100
MS.gene53239.t1 AT1G32180 23.225 521 341 8 155 619 427 944 2.85e-48 182
MS.gene53239.t1 AT1G32180 41.406 128 63 1 2 117 155 282 6.45e-24 107
MS.gene53239.t1 AT1G32180 23.225 521 341 8 155 619 427 944 2.85e-48 182
MS.gene53239.t1 AT1G32180 41.406 128 63 1 2 117 155 282 6.45e-24 107
MS.gene53239.t1 AT4G15320 27.950 483 258 13 150 568 288 744 8.24e-44 168
MS.gene53239.t1 AT4G15320 45.299 117 59 2 3 116 56 170 2.95e-25 111

Find 107 sgRNAs with CRISPR-Local

Find 275 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TTGAGAGGTCCTGGTCTTTC+TGG 0.082152 3.3:+973367 MS.gene53239:CDS
GCTATTGGATTCATACTTTA+TGG 0.096303 3.3:+975510 MS.gene53239:CDS
TTTGCTTCTTTGGTGGTTTA+TGG 0.144769 3.3:+976498 MS.gene53239:CDS
GCAATATATTTAGAGGTTTC+TGG 0.151089 3.3:-973123 None:intergenic
GTGAACACTATTTGCTTCTT+TGG 0.183474 3.3:+976488 MS.gene53239:CDS
TAAACAACACGTGGAATTTC+TGG 0.183636 3.3:-972827 None:intergenic
CTTGAAAATCCATAAGTAAA+AGG 0.205743 3.3:-975432 None:intergenic
AATAGAAGTGCATTGTTATT+TGG 0.211328 3.3:+973406 MS.gene53239:CDS
CATTTGATAGAGGTCATTTC+TGG 0.217342 3.3:+976227 MS.gene53239:CDS
CTACTCAATTTGCTAAAGTT+TGG 0.265706 3.3:+971772 MS.gene53239:CDS
AACAAATGCAATATATTTAG+AGG 0.277003 3.3:-973130 None:intergenic
TATGAATCCAATAGCATATA+TGG 0.280573 3.3:-975502 None:intergenic
TAGACTTGCCAAAGTAGTTT+TGG 0.285658 3.3:-974569 None:intergenic
GGTGGGATGAACAAAGAATT+TGG 0.291492 3.3:+976270 MS.gene53239:CDS
ACAGGTCAAGTACGAGAAAA+TGG 0.301944 3.3:+972311 MS.gene53239:intron
ATTTCATCCATATATGCTAT+TGG 0.307281 3.3:+975495 MS.gene53239:CDS
AAAGAAACACATGGCTTGTT+TGG 0.307493 3.3:-973094 None:intergenic
TCTATTTATCTTTCTGATGA+TGG 0.311528 3.3:+971714 MS.gene53239:CDS
TAAATAGAAAGCTTATTAGA+AGG 0.312123 3.3:-971699 None:intergenic
TACTCAATTTGCTAAAGTTT+GGG 0.342067 3.3:+971773 MS.gene53239:CDS
TTTGGAAGTCCAATTCAAAA+AGG 0.344261 3.3:+973424 MS.gene53239:CDS
CTTGATGCCCAAAACTACTT+TGG 0.395964 3.3:+974561 MS.gene53239:CDS
CAAAGAAGGCATGCTTCAAT+TGG 0.430014 3.3:+975362 MS.gene53239:CDS
TGTTGTTTCTGCTATTGCTA+TGG 0.430946 3.3:+971671 MS.gene53239:CDS
ATAGAGGTCATTTCTGGTGA+TGG 0.431524 3.3:+976233 MS.gene53239:CDS
TTTCCTGGTTAGCTATGTAA+TGG 0.435646 3.3:+976565 MS.gene53239:CDS
TGGTTGCAGCTAAGAGTCTC+AGG 0.440382 3.3:+973003 MS.gene53239:intron
GAGGCAGTTAAGAAATGGTT+AGG 0.443261 3.3:+976329 MS.gene53239:CDS
CCGGCCTCCATCGGTATGCT+TGG 0.446486 3.3:-971533 None:intergenic
AACACTATTTGCTTCTTTGG+TGG 0.448242 3.3:+976491 MS.gene53239:CDS
CTACGAATCAAACACACAAT+GGG 0.452008 3.3:+974698 MS.gene53239:CDS
CTAAGGTTGTGAAACATTTG+AGG 0.452359 3.3:-973159 None:intergenic
TTGAAAATCCATAAGTAAAA+GGG 0.454918 3.3:-975431 None:intergenic
TGACAGAGGCAGTTAAGAAA+TGG 0.472408 3.3:+976324 MS.gene53239:CDS
ACCGGCTGACGAGAAGTTAC+CGG 0.481496 3.3:+971584 MS.gene53239:CDS
CCCGGTAACTTCTCGTCAGC+CGG 0.486188 3.3:-971585 None:intergenic
ATGGATGGATTGAGAGGTCC+TGG 0.486201 3.3:+973358 MS.gene53239:CDS
GGTGATGATCGAGAAATTAC+CGG 0.486468 3.3:+971566 MS.gene53239:CDS
ATGGCTTGTTTGGCTGAAGA+TGG 0.488092 3.3:-973084 None:intergenic
TTTACGTGTCTCGTGAAAGA+AGG 0.490832 3.3:+972846 MS.gene53239:CDS
TCACAGATAGGATTCTCATA+TGG 0.490844 3.3:+975228 MS.gene53239:intron
CAATCCACTGCAAGTACATA+AGG 0.491845 3.3:-973042 None:intergenic
ACATTTATGATAATCAATCT+AGG 0.492978 3.3:+973190 MS.gene53239:CDS
AAATAGAAAGCTTATTAGAA+GGG 0.495228 3.3:-971698 None:intergenic
GGTCCTGGTCTTTCTGGCAC+TGG 0.499821 3.3:+973373 MS.gene53239:CDS
TAAGGTTGTGAAACATTTGA+GGG 0.508349 3.3:-973158 None:intergenic
ATGCCTTCTTTGATATCAGT+TGG 0.510191 3.3:-975351 None:intergenic
TGTTACAGGTCACAGACCCT+TGG 0.513094 3.3:+976162 MS.gene53239:intron
GATGGTTTGTTGTCCTCGGA+TGG 0.514250 3.3:-974616 None:intergenic
ACAAATTCAACATTTGATAG+AGG 0.517780 3.3:+976217 MS.gene53239:CDS
TGGTGACTGATAGACCTTCT+CGG 0.518496 3.3:+972379 MS.gene53239:CDS
CCTCAAGTCTGCTTCTTGAA+AGG 0.530046 3.3:+975540 MS.gene53239:CDS
ACAATGTGGCAAGGGATGGA+TGG 0.530205 3.3:+973343 MS.gene53239:CDS
CCATTGTGTGTTTGATTCGT+AGG 0.531038 3.3:-974697 None:intergenic
TATTTCAGACAATGTGGCAA+GGG 0.537874 3.3:+973335 MS.gene53239:intron
CCGGCTGACGAGAAGTTACC+GGG 0.539126 3.3:+971585 MS.gene53239:CDS
ACTATGAGTAACACAACCAT+TGG 0.541281 3.3:-976467 None:intergenic
TTGAATTTGTGAGGAAGTGA+TGG 0.541973 3.3:-972869 None:intergenic
AAGTACACACCAATGTATTG+AGG 0.543839 3.3:-972901 None:intergenic
AGTACACACCAATGTATTGA+GGG 0.544381 3.3:-972900 None:intergenic
TAACTATGAAAAGTAAGAGT+GGG 0.548538 3.3:+976618 MS.gene53239:CDS
GTCTCAGGTTTAATCAGCAA+TGG 0.549566 3.3:+973018 MS.gene53239:CDS
ATCGACTCACCGGCCTCCAT+CGG 0.559180 3.3:-971542 None:intergenic
TGTAAAGTCAAGGTGTCCTA+AGG 0.559519 3.3:+971818 MS.gene53239:CDS
CTTGAGTGATTCTATGTATG+TGG 0.560568 3.3:-974592 None:intergenic
ATGGCTCAGTGTCAATGTGG+TGG 0.563672 3.3:+976252 MS.gene53239:CDS
TCAGACAATGTGGCAAGGGA+TGG 0.566411 3.3:+973339 MS.gene53239:intron
CCTACGAATCAAACACACAA+TGG 0.567795 3.3:+974697 MS.gene53239:CDS
TCAAGAAATATGAACAAGGT+AGG 0.568930 3.3:+976411 MS.gene53239:CDS
TGGCTCAGTGTCAATGTGGT+GGG 0.570848 3.3:+976253 MS.gene53239:CDS
TCAACCAAGCATACCGATGG+AGG 0.570936 3.3:+971529 MS.gene53239:CDS
ATAACTATGAAAAGTAAGAG+TGG 0.571693 3.3:+976617 MS.gene53239:CDS
GTGTTCATAACTTCAACAGT+TGG 0.573512 3.3:-971648 None:intergenic
GAGCCATTACATAGCTAACC+AGG 0.577568 3.3:-976568 None:intergenic
AGGAATATCTGTGTTTCCAA+AGG 0.584748 3.3:+975560 MS.gene53239:CDS
GCAATTTATTTCAGACAATG+TGG 0.587290 3.3:+973329 MS.gene53239:intron
GGATTTACCTCGATCCGAGA+AGG 0.587807 3.3:-972393 None:intergenic
CCAAGCATACCGATGGAGGC+CGG 0.589912 3.3:+971533 MS.gene53239:CDS
AGCTTTGTACATGTCTCCAA+TGG 0.591743 3.3:+976451 MS.gene53239:CDS
CCTTTCAAGAAGCAGACTTG+AGG 0.591846 3.3:-975540 None:intergenic
CACACAAATATGTCGAGTCC+CGG 0.593041 3.3:-971603 None:intergenic
GCATGCTTCAATTGGTGAAG+TGG 0.593888 3.3:+975370 MS.gene53239:CDS
TCACTTCCTCACAAATTCAA+AGG 0.597910 3.3:+972872 MS.gene53239:CDS
GCACCTCCTTTGAATTTGTG+AGG 0.599322 3.3:-972878 None:intergenic
TGGACCTTATGTACTTGCAG+TGG 0.600660 3.3:+973038 MS.gene53239:CDS
GCACCAACTGATATCAAAGA+AGG 0.601020 3.3:+975348 MS.gene53239:CDS
TTATTTCAGACAATGTGGCA+AGG 0.603544 3.3:+973334 MS.gene53239:intron
ATGGAGGCCGGTGAGTCGAT+CGG 0.604807 3.3:+971545 MS.gene53239:CDS
TATCAAATGTTGAATTTGTG+TGG 0.607267 3.3:-976213 None:intergenic
TTACTAGAGAGTACTATCAC+TGG 0.610277 3.3:+975255 MS.gene53239:CDS
AGGAGGTGCCCTCAATACAT+TGG 0.621577 3.3:+972892 MS.gene53239:CDS
CAAGGGATGGATGGATTGAG+AGG 0.624702 3.3:+973352 MS.gene53239:CDS
CGAGACACGTAAACAACACG+TGG 0.627556 3.3:-972836 None:intergenic
TTACCAGTGCCAGAAAGACC+AGG 0.629357 3.3:-973376 None:intergenic
ATCATCACCGATCGACTCAC+CGG 0.630972 3.3:-971552 None:intergenic
AATGCAAACACTGCAAACCA+AGG 0.637039 3.3:-976179 None:intergenic
CACATACATAGAATCACTCA+AGG 0.639480 3.3:+974593 MS.gene53239:CDS
ATGCAAACACTGCAAACCAA+GGG 0.640787 3.3:-976178 None:intergenic
TGATAGACCTTCTCGGATCG+AGG 0.649181 3.3:+972386 MS.gene53239:CDS
GAATCACTCAAGGCCATCCG+AGG 0.649360 3.3:+974603 MS.gene53239:CDS
TAGTTTGTTTGCAATGACAG+AGG 0.658463 3.3:+976310 MS.gene53239:CDS
CTTCCTCACAAATTCAAAGG+AGG 0.671490 3.3:+972875 MS.gene53239:CDS
TGGATCAAGAAAGAAACACA+TGG 0.680933 3.3:-973103 None:intergenic
GTGATGGCTCAGTGTCAATG+TGG 0.686344 3.3:+976249 MS.gene53239:CDS
GGTGCACACCCTAAGAAACA+TGG 0.694506 3.3:-975330 None:intergenic
AAAATCAAGAAATATGAACA+AGG 0.697470 3.3:+976407 MS.gene53239:CDS
TACGAATCAAACACACAATG+GGG 0.714112 3.3:+974699 MS.gene53239:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! AATCAACAAAATATTAATTA+AGG + chr3.3:973228-973247 MS.gene53239:intron 10.0%
!! AGATATAATTAATTAATGTT+TGG + chr3.3:972257-972276 MS.gene53239:intron 10.0%
!! ATAAGAATAAAAATAAATGA+AGG + chr3.3:973952-973971 MS.gene53239:intron 10.0%
!! GAAATTTGAAAAATAAAAAT+AGG + chr3.3:976019-976038 MS.gene53239:intron 10.0%
!!! TATTTTATTTTTCTTTTCTA+AGG + chr3.3:971924-971943 MS.gene53239:intron 10.0%
!! ATGATTGCAAAATAATAATT+AGG - chr3.3:973459-973478 None:intergenic 15.0%
!! CTTATATATGAAACAATAAA+TGG - chr3.3:975964-975983 None:intergenic 15.0%
!! TAAAATAAAATACCTTAATC+TGG - chr3.3:971911-971930 None:intergenic 15.0%
!! TACAATTTACAATTTACATT+CGG - chr3.3:972655-972674 None:intergenic 15.0%
!! TGAAAAAATATCAATCAATT+CGG - chr3.3:974966-974985 None:intergenic 15.0%
!! TTGAATATATAATTTACCTT+TGG - chr3.3:975579-975598 None:intergenic 15.0%
!!! ATTTTACAAGTTTATTTTGT+AGG + chr3.3:973868-973887 MS.gene53239:intron 15.0%
!!! GATTAACTATTTTTTAAATG+TGG + chr3.3:972575-972594 MS.gene53239:intron 15.0%
!!! TGTGTAAACTTTTAAAATTT+TGG - chr3.3:974889-974908 None:intergenic 15.0%
!!! TTTTATTGTTGTTAAAGATT+GGG + chr3.3:972734-972753 MS.gene53239:intron 15.0%
!! AAAATCAAGAAATATGAACA+AGG + chr3.3:976407-976426 MS.gene53239:CDS 20.0%
!! AAATAGAAAGCTTATTAGAA+GGG - chr3.3:971701-971720 None:intergenic 20.0%
!! AAATCAACCACATAAAAATT+TGG + chr3.3:974434-974453 MS.gene53239:intron 20.0%
!! AAATTGTCTTCACTTATAAA+CGG + chr3.3:974378-974397 MS.gene53239:intron 20.0%
!! AACAAATGCAATATATTTAG+AGG - chr3.3:973133-973152 None:intergenic 20.0%
!! AACTACATGAAATCTTATAA+TGG - chr3.3:972110-972129 None:intergenic 20.0%
!! AATGATTATTAGTCCTTTAT+TGG - chr3.3:972417-972436 None:intergenic 20.0%
!! ACATGTTAAATTATAAAACC+AGG - chr3.3:976129-976148 None:intergenic 20.0%
!! ACATTTATGATAATCAATCT+AGG + chr3.3:973190-973209 MS.gene53239:CDS 20.0%
!! ACTACATGAAATCTTATAAT+GGG - chr3.3:972109-972128 None:intergenic 20.0%
!! ATTAACCAACTTATTACAAA+TGG - chr3.3:972536-972555 None:intergenic 20.0%
!! GACTAAAATATCTATTTACT+TGG - chr3.3:974771-974790 None:intergenic 20.0%
!! GAGAAAAATATTGAGAAATT+CGG + chr3.3:972333-972352 MS.gene53239:CDS 20.0%
!! GATACATTTATCAAAATCTA+GGG + chr3.3:974740-974759 MS.gene53239:intron 20.0%
!! TAAATAGAAAGCTTATTAGA+AGG - chr3.3:971702-971721 None:intergenic 20.0%
!! TATTACTATTTCGTAAATTG+AGG + chr3.3:974999-975018 MS.gene53239:intron 20.0%
!! TCCAATCATAATAAAATTCA+TGG - chr3.3:975707-975726 None:intergenic 20.0%
!! TGATACATTTATCAAAATCT+AGG + chr3.3:974739-974758 MS.gene53239:intron 20.0%
!! TTGAAAATCCATAAGTAAAA+GGG - chr3.3:975434-975453 None:intergenic 20.0%
!!! AAAGAAAAGTACTCTTATAT+TGG - chr3.3:974518-974537 None:intergenic 20.0%
!!! AAATAGTCCAAATTTTTATG+TGG - chr3.3:974444-974463 None:intergenic 20.0%
!!! AAATATGTACCTTTTTGAAT+TGG - chr3.3:973436-973455 None:intergenic 20.0%
!!! AAATTCATGGTTTTAAATTG+CGG - chr3.3:975694-975713 None:intergenic 20.0%
!!! ACAACTAAAAACCTAATTTT+AGG - chr3.3:974054-974073 None:intergenic 20.0%
!!! ACACCATATTTTTTACAAAA+GGG - chr3.3:971801-971820 None:intergenic 20.0%
!!! ACCATGAATTTTATTATGAT+TGG + chr3.3:975703-975722 MS.gene53239:intron 20.0%
!!! ACTATATGTGGTATATTTTT+GGG - chr3.3:975127-975146 None:intergenic 20.0%
!!! ATTATTCAATGTAATTTGTG+AGG - chr3.3:972444-972463 None:intergenic 20.0%
!!! ATTTTGTACAACAATTTTAG+AGG - chr3.3:974931-974950 None:intergenic 20.0%
!!! CATGTTTTATAGATAGTAAT+AGG - chr3.3:975066-975085 None:intergenic 20.0%
!!! CTTTTATTGTTGTTAAAGAT+TGG + chr3.3:972733-972752 MS.gene53239:intron 20.0%
!!! TACACCATATTTTTTACAAA+AGG - chr3.3:971802-971821 None:intergenic 20.0%
!!! TACTATATGTGGTATATTTT+TGG - chr3.3:975128-975147 None:intergenic 20.0%
!!! TTGAATTTTTATTCACAGAT+AGG + chr3.3:975216-975235 MS.gene53239:intron 20.0%
!!! TTTCACTCATTTTTTTATTG+TGG + chr3.3:972983-973002 MS.gene53239:intron 20.0%
!!! TTTGATGATCATATAAATGA+TGG + chr3.3:973300-973319 MS.gene53239:intron 20.0%
!!! TTTTAATTTACTGTTGTTAC+AGG + chr3.3:976148-976167 MS.gene53239:intron 20.0%
!!! TTTTGAAAGATTCTTTTTGA+TGG - chr3.3:974637-974656 None:intergenic 20.0%
! AAAAATATGGTGTAAAGTCA+AGG + chr3.3:971808-971827 MS.gene53239:CDS 25.0%
! ACAAATTCAACATTTGATAG+AGG + chr3.3:976217-976236 MS.gene53239:CDS 25.0%
! AGTAAATTGTAAACTGTAAG+TGG + chr3.3:972602-972621 MS.gene53239:intron 25.0%
! AGTTTACACATAACATAACA+AGG + chr3.3:974899-974918 MS.gene53239:intron 25.0%
! ATAACTATGAAAAGTAAGAG+TGG + chr3.3:976617-976636 MS.gene53239:CDS 25.0%
! ATATGAGAGAAAAAAACCTT+AGG - chr3.3:971837-971856 None:intergenic 25.0%
! ATTTCATCCATATATGCTAT+TGG + chr3.3:975495-975514 MS.gene53239:CDS 25.0%
! CAAAAAGAATCTTTCAAAAG+AGG + chr3.3:974638-974657 MS.gene53239:CDS 25.0%
! CTTGAAAATCCATAAGTAAA+AGG - chr3.3:975435-975454 None:intergenic 25.0%
! GTACTATTCCTCTTAAAATT+TGG - chr3.3:973906-973925 None:intergenic 25.0%
! GTTAACCATTTGTAATAAGT+TGG + chr3.3:972528-972547 MS.gene53239:intron 25.0%
! GTTTACACATAACATAACAA+GGG + chr3.3:974900-974919 MS.gene53239:intron 25.0%
! TAAAGATTGGGAAAAAAAGA+AGG + chr3.3:972746-972765 MS.gene53239:intron 25.0%
! TAACTATGAAAAGTAAGAGT+GGG + chr3.3:976618-976637 MS.gene53239:CDS 25.0%
! TAGTTAAAGACAAATCGTTT+AGG - chr3.3:975888-975907 None:intergenic 25.0%
! TATCAAATGTTGAATTTGTG+TGG - chr3.3:976216-976235 None:intergenic 25.0%
! TATGAATCCAATAGCATATA+TGG - chr3.3:975505-975524 None:intergenic 25.0%
! TCTATTTATCTTTCTGATGA+TGG + chr3.3:971714-971733 MS.gene53239:CDS 25.0%
! TTACATGACAAGTAATAATG+TGG - chr3.3:973547-973566 None:intergenic 25.0%
!! AATAGAAGTGCATTGTTATT+TGG + chr3.3:973406-973425 MS.gene53239:CDS 25.0%
!! CTTAATCACAAATTCTAAGA+TGG - chr3.3:974322-974341 None:intergenic 25.0%
!! GATCATATTTTGTCTAATGT+AGG + chr3.3:974400-974419 MS.gene53239:intron 25.0%
!! GTTCCCTTTTGTAAAAAATA+TGG + chr3.3:971795-971814 MS.gene53239:CDS 25.0%
!! TAGTAAGTAAATTTTTGTCC+TGG + chr3.3:976108-976127 MS.gene53239:intron 25.0%
!! TCTATTTAAAATTGCGATTG+TGG + chr3.3:975610-975629 MS.gene53239:intron 25.0%
!! TGTCGATCTTTACAATTTTA+CGG - chr3.3:974793-974812 None:intergenic 25.0%
!!! AATTTTGGTGCAAAAATTTG+AGG - chr3.3:974874-974893 None:intergenic 25.0%
!!! CATAAATGTCTTTTTTGCTA+AGG - chr3.3:973179-973198 None:intergenic 25.0%
!!! GTTAGTTTGTTAATTAGTTG+AGG - chr3.3:972714-972733 None:intergenic 25.0%
!!! GTTGGTTTTTACAATTTAAC+AGG + chr3.3:972293-972312 MS.gene53239:intron 25.0%
!!! TAAGGTTTTTTTCTCTCATA+TGG + chr3.3:971836-971855 MS.gene53239:intron 25.0%
!!! TACTCAATTTGCTAAAGTTT+GGG + chr3.3:971773-971792 MS.gene53239:CDS 25.0%
AAATGCTAACTTCAATGTTG+TGG - chr3.3:974487-974506 None:intergenic 30.0%
AAGAGGAATAGTACATAACT+AGG + chr3.3:973912-973931 MS.gene53239:intron 30.0%
ATGTAAAAACCAAGTGATTG+AGG - chr3.3:975930-975949 None:intergenic 30.0%
ATTATCTACTAGCAGCTTAA+AGG - chr3.3:974017-974036 None:intergenic 30.0%
CAAAAACACCATGTTTCTTA+GGG + chr3.3:975322-975341 MS.gene53239:CDS 30.0%
GATCAACTGAATACTATATG+TGG - chr3.3:975139-975158 None:intergenic 30.0%
GATTGCAAAAAATGAATGTG+TGG - chr3.3:973505-973524 None:intergenic 30.0%
GCAATATATTTAGAGGTTTC+TGG - chr3.3:973126-973145 None:intergenic 30.0%
GCAATTTATTTCAGACAATG+TGG + chr3.3:973329-973348 MS.gene53239:intron 30.0%
GCTATTGGATTCATACTTTA+TGG + chr3.3:975510-975529 MS.gene53239:CDS 30.0%
GTAAAATCTCTTAGAACTTG+TGG + chr3.3:974095-974114 MS.gene53239:intron 30.0%
GTTATAATCCCCTCAAAAAT+TGG - chr3.3:976602-976621 None:intergenic 30.0%
GTTTACATTGTGGTAAAATG+CGG + chr3.3:975646-975665 MS.gene53239:intron 30.0%
TAAGGTTGTGAAACATTTGA+GGG - chr3.3:973161-973180 None:intergenic 30.0%
TACAAAAAGTTTAGACGTGT+TGG - chr3.3:973766-973785 None:intergenic 30.0%
TACTATTTCGTAAATTGAGG+AGG + chr3.3:975002-975021 MS.gene53239:intron 30.0%
TCAAGAAATATGAACAAGGT+AGG + chr3.3:976411-976430 MS.gene53239:CDS 30.0%
TTCTCTTCTTTAAACAAGTG+AGG + chr3.3:971952-971971 MS.gene53239:intron 30.0%
TTTGGAAGTCCAATTCAAAA+AGG + chr3.3:973424-973443 MS.gene53239:CDS 30.0%
! AAATACAGCCCTTTTACTTA+TGG + chr3.3:975423-975442 MS.gene53239:CDS 30.0%
! AGCTTATTCTATCAGTTTTG+TGG + chr3.3:971502-971521 MS.gene53239:CDS 30.0%
! CTAAGAAACATGGTGTTTTT+GGG - chr3.3:975323-975342 None:intergenic 30.0%
! CTTAGCTATCCAATTTTTGA+GGG + chr3.3:976590-976609 MS.gene53239:CDS 30.0%
! GATCACAACCAAATTTTAAG+AGG + chr3.3:973895-973914 MS.gene53239:intron 30.0%
! GCTAGTAGATAATTTTTGCT+AGG + chr3.3:974024-974043 MS.gene53239:intron 30.0%
! TCTTAGCTATCCAATTTTTG+AGG + chr3.3:976589-976608 MS.gene53239:CDS 30.0%
! TTCTTTTGAGCGTTACAATT+TGG - chr3.3:972147-972166 None:intergenic 30.0%
!! AGAGATTTTGAAGACATGTT+TGG + chr3.3:976536-976555 MS.gene53239:CDS 30.0%
!! CACTTGGTTTTTACATGAAA+CGG + chr3.3:975934-975953 MS.gene53239:intron 30.0%
!! CAGTTTTGTGTAGTTATGTT+AGG - chr3.3:974345-974364 None:intergenic 30.0%
!! CTACTCAATTTGCTAAAGTT+TGG + chr3.3:971772-971791 MS.gene53239:CDS 30.0%
!! GGCATTTTAGTCATTGAATT+TGG - chr3.3:975045-975064 None:intergenic 30.0%
!! TTAGCTATCCAATTTTTGAG+GGG + chr3.3:976591-976610 MS.gene53239:CDS 30.0%
!! TTCAACAGTTGGTTCTTTTT+CGG - chr3.3:971640-971659 None:intergenic 30.0%
!!! CTTATTTTTTCAAACCAAGC+AGG - chr3.3:975730-975749 None:intergenic 30.0%
!!! TACTCTTTTTGGGATTAAAG+AGG + chr3.3:971749-971768 MS.gene53239:CDS 30.0%
AACACTATTTGCTTCTTTGG+TGG + chr3.3:976491-976510 MS.gene53239:CDS 35.0%
AAGTACACACCAATGTATTG+AGG - chr3.3:972904-972923 None:intergenic 35.0%
AATCTCTTAGAACTTGTGGT+TGG + chr3.3:974099-974118 MS.gene53239:intron 35.0%
ACTATGAGTAACACAACCAT+TGG - chr3.3:976470-976489 None:intergenic 35.0%
AGCTTCAGTTAAGATCTGTT+GGG - chr3.3:974293-974312 None:intergenic 35.0%
AGGAATATCTGTGTTTCCAA+AGG + chr3.3:975560-975579 MS.gene53239:CDS 35.0%
AGTACACACCAATGTATTGA+GGG - chr3.3:972903-972922 None:intergenic 35.0%
ATGACAAGAACTATTGAGCT+TGG + chr3.3:974229-974248 MS.gene53239:intron 35.0%
ATGCCTTCTTTGATATCAGT+TGG - chr3.3:975354-975373 None:intergenic 35.0%
CACATACATAGAATCACTCA+AGG + chr3.3:974593-974612 MS.gene53239:CDS 35.0%
CATTTGATAGAGGTCATTTC+TGG + chr3.3:976227-976246 MS.gene53239:CDS 35.0%
CCAAAAACACCATGTTTCTT+AGG + chr3.3:975321-975340 MS.gene53239:CDS 35.0%
CTAAGGTTGTGAAACATTTG+AGG - chr3.3:973162-973181 None:intergenic 35.0%
CTACGAATCAAACACACAAT+GGG + chr3.3:974698-974717 MS.gene53239:CDS 35.0%
CTCAATAGTTCTTGTCATGA+GGG - chr3.3:974227-974246 None:intergenic 35.0%
CTTGAGTGATTCTATGTATG+TGG - chr3.3:974595-974614 None:intergenic 35.0%
GATCGAGGTAAATCCAATAA+AGG + chr3.3:972401-972420 MS.gene53239:intron 35.0%
GTGAACACTATTTGCTTCTT+TGG + chr3.3:976488-976507 MS.gene53239:CDS 35.0%
GTGTTCATAACTTCAACAGT+TGG - chr3.3:971651-971670 None:intergenic 35.0%
GTTTGCAAACACAATTGCAT+CGG - chr3.3:975672-975691 None:intergenic 35.0%
TAAACAACACGTGGAATTTC+TGG - chr3.3:972830-972849 None:intergenic 35.0%
TAAGAGTCGTTGAATGATGT+AGG + chr3.3:973810-973829 MS.gene53239:intron 35.0%
TACGAATCAAACACACAATG+GGG + chr3.3:974699-974718 MS.gene53239:CDS 35.0%
TATTTCAGACAATGTGGCAA+GGG + chr3.3:973335-973354 MS.gene53239:intron 35.0%
TCAATAGTTCTTGTCATGAG+GGG - chr3.3:974226-974245 None:intergenic 35.0%
TCACAGATAGGATTCTCATA+TGG + chr3.3:975228-975247 MS.gene53239:intron 35.0%
TCACTTCCTCACAAATTCAA+AGG + chr3.3:972872-972891 MS.gene53239:CDS 35.0%
TGCTAGGTGTACCTAAAATT+AGG + chr3.3:974040-974059 MS.gene53239:intron 35.0%
TGGATCAAGAAAGAAACACA+TGG - chr3.3:973106-973125 None:intergenic 35.0%
TTATTATGATTGGACCTGCT+TGG + chr3.3:975713-975732 MS.gene53239:intron 35.0%
TTATTTCAGACAATGTGGCA+AGG + chr3.3:973334-973353 MS.gene53239:intron 35.0%
TTGAATTTGTGAGGAAGTGA+TGG - chr3.3:972872-972891 None:intergenic 35.0%
TTTGGATTCACATGACATGT+TGG + chr3.3:972275-972294 MS.gene53239:intron 35.0%
! AGACTTGCCAAAGTAGTTTT+GGG - chr3.3:974571-974590 None:intergenic 35.0%
! AGTCACCATACGAAGATTTT+TGG - chr3.3:972367-972386 None:intergenic 35.0%
! CCTAAGAAACATGGTGTTTT+TGG - chr3.3:975324-975343 None:intergenic 35.0%
! CTTAATCTTGATGACCCATT+AGG + chr3.3:973738-973757 MS.gene53239:intron 35.0%
! CTTCTGCTGTTACTCTTTTT+GGG + chr3.3:971739-971758 MS.gene53239:CDS 35.0%
! GTCACCATACGAAGATTTTT+GGG - chr3.3:972366-972385 None:intergenic 35.0%
! TAGACTTGCCAAAGTAGTTT+TGG - chr3.3:974572-974591 None:intergenic 35.0%
! TCACCATACGAAGATTTTTG+GGG - chr3.3:972365-972384 None:intergenic 35.0%
! TTTCCTGGTTAGCTATGTAA+TGG + chr3.3:976565-976584 MS.gene53239:CDS 35.0%
! TTTGCTTCTTTGGTGGTTTA+TGG + chr3.3:976498-976517 MS.gene53239:CDS 35.0%
! TTTTGAGCGTTACAATTTGG+AGG - chr3.3:972144-972163 None:intergenic 35.0%
!! AAAGAAACACATGGCTTGTT+TGG - chr3.3:973097-973116 None:intergenic 35.0%
!! CAAGGTAGGTTTGATTTTCA+AGG + chr3.3:976425-976444 MS.gene53239:CDS 35.0%
!! CAGTTTTATGAGGTTATGCT+AGG - chr3.3:974135-974154 None:intergenic 35.0%
!! CATGTTTCAAAGAGTATCGA+CGG - chr3.3:973710-973729 None:intergenic 35.0%
!! TAGTTTGTTTGCAATGACAG+AGG + chr3.3:976310-976329 MS.gene53239:CDS 35.0%
!! TCAACAGTTGGTTCTTTTTC+GGG - chr3.3:971639-971658 None:intergenic 35.0%
!! TCAATCTAGGACTGCTTTTA+AGG + chr3.3:973203-973222 MS.gene53239:CDS 35.0%
!! TGTTGTTTCTGCTATTGCTA+TGG + chr3.3:971671-971690 MS.gene53239:CDS 35.0%
!! TTACTAGAGAGTACTATCAC+TGG + chr3.3:975255-975274 MS.gene53239:CDS 35.0%
!!! AACATGGTGTTTTTGGGTAA+AGG - chr3.3:975317-975336 None:intergenic 35.0%
!!! TTTTGATGGTTTGTTGTCCT+CGG - chr3.3:974623-974642 None:intergenic 35.0%
!!! TTTTTTCTCTCATATGGCTG+AGG + chr3.3:971842-971861 MS.gene53239:intron 35.0%
AATGCAAACACTGCAAACCA+AGG - chr3.3:976182-976201 None:intergenic 40.0%
ACAATGGACCATTGGGAAAT+TGG + chr3.3:975796-975815 MS.gene53239:intron 40.0%
ACAGGTCAAGTACGAGAAAA+TGG + chr3.3:972311-972330 MS.gene53239:intron 40.0%
ATGCAAACACTGCAAACCAA+GGG - chr3.3:976181-976200 None:intergenic 40.0%
CAACGACTCTTAATAGCTTC+AGG - chr3.3:973802-973821 None:intergenic 40.0%
CAATCCACTGCAAGTACATA+AGG - chr3.3:973045-973064 None:intergenic 40.0%
CCTACGAATCAAACACACAA+TGG + chr3.3:974697-974716 MS.gene53239:CDS 40.0%
CTATGCTACAATGGACCATT+GGG + chr3.3:975789-975808 MS.gene53239:intron 40.0%
CTGAGGATGAACATGTTCTT+AGG + chr3.3:971859-971878 MS.gene53239:intron 40.0%
CTTCCTCACAAATTCAAAGG+AGG + chr3.3:972875-972894 MS.gene53239:CDS 40.0%
CTTGATGCCCAAAACTACTT+TGG + chr3.3:974561-974580 MS.gene53239:CDS 40.0%
GACTTCCATGTTGTCAAATG+TGG + chr3.3:975085-975104 MS.gene53239:intron 40.0%
GAGCTTCAGTTAAGATCTGT+TGG - chr3.3:974294-974313 None:intergenic 40.0%
GAGGCAGTTAAGAAATGGTT+AGG + chr3.3:976329-976348 MS.gene53239:CDS 40.0%
GCACCAACTGATATCAAAGA+AGG + chr3.3:975348-975367 MS.gene53239:CDS 40.0%
GCTCAATAGTTCTTGTCATG+AGG - chr3.3:974228-974247 None:intergenic 40.0%
GGTGATGATCGAGAAATTAC+CGG + chr3.3:971566-971585 MS.gene53239:CDS 40.0%
GGTGGGATGAACAAAGAATT+TGG + chr3.3:976270-976289 MS.gene53239:CDS 40.0%
GTCTCAGGTTTAATCAGCAA+TGG + chr3.3:973018-973037 MS.gene53239:CDS 40.0%
TAGACGTGTTGGATCCTAAT+GGG - chr3.3:973755-973774 None:intergenic 40.0%
TCTTAGGACACAAGAATTCG+AGG + chr3.3:971875-971894 MS.gene53239:intron 40.0%
TGACAGAGGCAGTTAAGAAA+TGG + chr3.3:976324-976343 MS.gene53239:CDS 40.0%
TTAGACGTGTTGGATCCTAA+TGG - chr3.3:973756-973775 None:intergenic 40.0%
TTTACGTGTCTCGTGAAAGA+AGG + chr3.3:972846-972865 MS.gene53239:CDS 40.0%
! AGCTTTGTACATGTCTCCAA+TGG + chr3.3:976451-976470 MS.gene53239:CDS 40.0%
! ATCTTTTGCACTGTAGAGGA+TGG + chr3.3:975280-975299 MS.gene53239:CDS 40.0%
! CAAAGAAGGCATGCTTCAAT+TGG + chr3.3:975362-975381 MS.gene53239:CDS 40.0%
! CATGTTTGGTCAACTTTTCC+TGG + chr3.3:976550-976569 MS.gene53239:CDS 40.0%
! CCATTGTGTGTTTGATTCGT+AGG - chr3.3:974700-974719 None:intergenic 40.0%
! GCTTCTGCTGTTACTCTTTT+TGG + chr3.3:971738-971757 MS.gene53239:CDS 40.0%
! TGTAAAGTCAAGGTGTCCTA+AGG + chr3.3:971818-971837 MS.gene53239:CDS 40.0%
! TTTTCAACCAAGCATACCGA+TGG + chr3.3:971526-971545 MS.gene53239:CDS 40.0%
!! ATAGAGGTCATTTCTGGTGA+TGG + chr3.3:976233-976252 MS.gene53239:CDS 40.0%
!! CCTCACAAGTCAGTTTTATG+AGG - chr3.3:974145-974164 None:intergenic 40.0%
!! CCTCATAAAACTGACTTGTG+AGG + chr3.3:974142-974161 MS.gene53239:intron 40.0%
!! CTACACAAAACTGACTTGTG+AGG + chr3.3:974352-974371 MS.gene53239:intron 40.0%
!! TAAAACTGACTTGTGAGGAG+AGG + chr3.3:974147-974166 MS.gene53239:intron 40.0%
!! TGACAGAGACTTGCTGATTA+AGG + chr3.3:973987-974006 MS.gene53239:intron 40.0%
!!! CATACGAAGATTTTTGGGGT+CGG - chr3.3:972361-972380 None:intergenic 40.0%
ACCAAGTGATTGAGGTCAAG+TGG - chr3.3:975922-975941 None:intergenic 45.0%
ACCACTTGACCTCAATCACT+TGG + chr3.3:975918-975937 MS.gene53239:intron 45.0%
ACCATTGGGAAATTGGAGGT+CGG + chr3.3:975803-975822 MS.gene53239:intron 45.0%
ATGGACCATTGGGAAATTGG+AGG + chr3.3:975799-975818 MS.gene53239:intron 45.0%
CACACAAATATGTCGAGTCC+CGG - chr3.3:971606-971625 None:intergenic 45.0%
CCACTCCACATTTGACAACA+TGG - chr3.3:975093-975112 None:intergenic 45.0%
CCATGTTGTCAAATGTGGAG+TGG + chr3.3:975090-975109 MS.gene53239:intron 45.0%
CCTCAAGTCTGCTTCTTGAA+AGG + chr3.3:975540-975559 MS.gene53239:CDS 45.0%
CCTTTCAAGAAGCAGACTTG+AGG - chr3.3:975543-975562 None:intergenic 45.0%
CGACCCCAAAAATCTTCGTA+TGG + chr3.3:972359-972378 MS.gene53239:CDS 45.0%
GAGCCATTACATAGCTAACC+AGG - chr3.3:976571-976590 None:intergenic 45.0%
GCACCTCCTTTGAATTTGTG+AGG - chr3.3:972881-972900 None:intergenic 45.0%
GCATGCTTCAATTGGTGAAG+TGG + chr3.3:975370-975389 MS.gene53239:CDS 45.0%
TATCGACGGATGAACACTTG+AGG - chr3.3:973696-973715 None:intergenic 45.0%
TCTCACTCTTGAGCTGATCT+AGG + chr3.3:974073-974092 MS.gene53239:intron 45.0%
TGCTGCGAACGTTTACATTG+TGG + chr3.3:975636-975655 MS.gene53239:intron 45.0%
TGGACCTTATGTACTTGCAG+TGG + chr3.3:973038-973057 MS.gene53239:CDS 45.0%
TGGTGACTGATAGACCTTCT+CGG + chr3.3:972379-972398 MS.gene53239:CDS 45.0%
TGGTTCGTGGACATAAATGG+TGG + chr3.3:974249-974268 MS.gene53239:intron 45.0%
! GAACTATTGAGCTTGGTTCG+TGG + chr3.3:974236-974255 MS.gene53239:intron 45.0%
! GCTTGGTTCGTGGACATAAA+TGG + chr3.3:974246-974265 MS.gene53239:intron 45.0%
! GGCTATCTTTTGCACTGTAG+AGG + chr3.3:975276-975295 MS.gene53239:CDS 45.0%
! TCGTGGACATAAATGGTGGA+TGG + chr3.3:974253-974272 MS.gene53239:intron 45.0%
!! ATGGCTTGTTTGGCTGAAGA+TGG - chr3.3:973087-973106 None:intergenic 45.0%
!! AAATTTGAAAAATAAAAATA+GGG + chr3.3:976020-976039 MS.gene53239:intron 5.0%
!!! TTATATAATAATTTTTTTTG+TGG + chr3.3:972947-972966 MS.gene53239:intron 5.0%
ACAATGTGGCAAGGGATGGA+TGG + chr3.3:973343-973362 MS.gene53239:CDS 50.0%
ATGGCTCAGTGTCAATGTGG+TGG + chr3.3:976252-976271 MS.gene53239:CDS 50.0%
CCATTGGGAAATTGGAGGTC+GGG + chr3.3:975804-975823 MS.gene53239:intron 50.0%
CGAGACACGTAAACAACACG+TGG - chr3.3:972839-972858 None:intergenic 50.0%
GGATTTACCTCGATCCGAGA+AGG - chr3.3:972396-972415 None:intergenic 50.0%
GGCAGAGAGAGACCAGATTA+AGG + chr3.3:971896-971915 MS.gene53239:intron 50.0%
GGTGCACACCCTAAGAAACA+TGG - chr3.3:975333-975352 None:intergenic 50.0%
GTGATGGCTCAGTGTCAATG+TGG + chr3.3:976249-976268 MS.gene53239:CDS 50.0%
TCAACCAAGCATACCGATGG+AGG + chr3.3:971529-971548 MS.gene53239:CDS 50.0%
TCAGACAATGTGGCAAGGGA+TGG + chr3.3:973339-973358 MS.gene53239:intron 50.0%
TGATAGACCTTCTCGGATCG+AGG + chr3.3:972386-972405 MS.gene53239:CDS 50.0%
TGGCTCAGTGTCAATGTGGT+GGG + chr3.3:976253-976272 MS.gene53239:CDS 50.0%
TGGTTGCAGCTAAGAGTCTC+AGG + chr3.3:973003-973022 MS.gene53239:intron 50.0%
TGTTACAGGTCACAGACCCT+TGG + chr3.3:976162-976181 MS.gene53239:intron 50.0%
TTACCAGTGCCAGAAAGACC+AGG - chr3.3:973379-973398 None:intergenic 50.0%
! ATCATCACCGATCGACTCAC+CGG - chr3.3:971555-971574 None:intergenic 50.0%
! GAACTTGTGGTTGGTGTGCT+TGG + chr3.3:974108-974127 MS.gene53239:intron 50.0%
! TTGAGAGGTCCTGGTCTTTC+TGG + chr3.3:973367-973386 MS.gene53239:CDS 50.0%
!! AGGAGGTGCCCTCAATACAT+TGG + chr3.3:972892-972911 MS.gene53239:CDS 50.0%
!! ATGGATGGATTGAGAGGTCC+TGG + chr3.3:973358-973377 MS.gene53239:CDS 50.0%
!! CAAGGGATGGATGGATTGAG+AGG + chr3.3:973352-973371 MS.gene53239:CDS 50.0%
!! GATGGTTTGTTGTCCTCGGA+TGG - chr3.3:974619-974638 None:intergenic 50.0%
ACCGGCTGACGAGAAGTTAC+CGG + chr3.3:971584-971603 MS.gene53239:CDS 55.0%
CCCGACCTCCAATTTCCCAA+TGG - chr3.3:975807-975826 None:intergenic 55.0%
GAATCACTCAAGGCCATCCG+AGG + chr3.3:974603-974622 MS.gene53239:CDS 55.0%
TTGGGAAATTGGAGGTCGGG+TGG + chr3.3:975807-975826 MS.gene53239:intron 55.0%
ATGGAGGCCGGTGAGTCGAT+CGG + chr3.3:971545-971564 MS.gene53239:CDS 60.0%
CCAAGCATACCGATGGAGGC+CGG + chr3.3:971533-971552 MS.gene53239:CDS 60.0%
CCCGGTAACTTCTCGTCAGC+CGG - chr3.3:971588-971607 None:intergenic 60.0%
CCGGCTGACGAGAAGTTACC+GGG + chr3.3:971585-971604 MS.gene53239:CDS 60.0%
! ATCGACTCACCGGCCTCCAT+CGG - chr3.3:971545-971564 None:intergenic 60.0%
! GGTCCTGGTCTTTCTGGCAC+TGG + chr3.3:973373-973392 MS.gene53239:CDS 60.0%
CCGGCCTCCATCGGTATGCT+TGG - chr3.3:971536-971555 None:intergenic 65.0%


Chromosome Type Strat End Strand Name
chr3.3 gene 971489 976643 971489 ID=MS.gene53239
chr3.3 mRNA 971489 976643 971489 ID=MS.gene53239.t1;Parent=MS.gene53239
chr3.3 exon 971489 971839 971489 ID=MS.gene53239.t1.exon1;Parent=MS.gene53239.t1
chr3.3 CDS 971489 971839 971489 ID=cds.MS.gene53239.t1;Parent=MS.gene53239.t1
chr3.3 exon 972315 972407 972315 ID=MS.gene53239.t1.exon2;Parent=MS.gene53239.t1
chr3.3 CDS 972315 972407 972315 ID=cds.MS.gene53239.t1;Parent=MS.gene53239.t1
chr3.3 exon 972812 972913 972812 ID=MS.gene53239.t1.exon3;Parent=MS.gene53239.t1
chr3.3 CDS 972812 972913 972812 ID=cds.MS.gene53239.t1;Parent=MS.gene53239.t1
chr3.3 exon 973012 973224 973012 ID=MS.gene53239.t1.exon4;Parent=MS.gene53239.t1
chr3.3 CDS 973012 973224 973012 ID=cds.MS.gene53239.t1;Parent=MS.gene53239.t1
chr3.3 exon 973343 973445 973343 ID=MS.gene53239.t1.exon5;Parent=MS.gene53239.t1
chr3.3 CDS 973343 973445 973343 ID=cds.MS.gene53239.t1;Parent=MS.gene53239.t1
chr3.3 exon 974550 974728 974550 ID=MS.gene53239.t1.exon6;Parent=MS.gene53239.t1
chr3.3 CDS 974550 974728 974550 ID=cds.MS.gene53239.t1;Parent=MS.gene53239.t1
chr3.3 exon 975234 975581 975234 ID=MS.gene53239.t1.exon7;Parent=MS.gene53239.t1
chr3.3 CDS 975234 975581 975234 ID=cds.MS.gene53239.t1;Parent=MS.gene53239.t1
chr3.3 exon 976170 976643 976170 ID=MS.gene53239.t1.exon8;Parent=MS.gene53239.t1
chr3.3 CDS 976170 976643 976170 ID=cds.MS.gene53239.t1;Parent=MS.gene53239.t1
Gene Sequence

>MS.gene53239

ATGACACTAGCTGAGCTTATTCTATCAGTTTTGTGGTTTTTCAACCAAGCATACCGATGGAGGCCGGTGAGTCGATCGGTGATGATCGAGAAATTACCGGCTGACGAGAAGTTACCGGGACTCGACATATTTGTGTGTACAATTGATCCCGAAAAAGAACCAACTGTTGAAGTTATGAACACTGTTGTTTCTGCTATTGCTATGGATTACCCTTCTAATAAGCTTTCTATTTATCTTTCTGATGATGGTGCTTCTGCTGTTACTCTTTTTGGGATTAAAGAGGCTACTCAATTTGCTAAAGTTTGGGTTCCCTTTTGTAAAAAATATGGTGTAAAGTCAAGGTGTCCTAAGGTTTTTTTCTCTCATATGGCTGAGGATGAACATGTTCTTAGGACACAAGAATTCGAGGCAGAGAGAGACCAGATTAAGGTATTTTATTTTATTTTTCTTTTCTAAGGTTTTTTTCTCTTCTTTAAACAAGTGAGGTAGTAGTAGATACAATTAAATTCAACTCATGCTTTTATGTTCATTAATTGACGTAACAACATATTATTAATTACGTATGTAAAATAATTTTACACTGTTGTGTATTGCAATTAAACTCTTATAGTATATATCCCATTATAAGATTTCATGTAGTTAGTTTTCTTTTCCTCCAAATTGTAACGCTCAAAAGAAGTGATCTAGATTGATGTATGTAGACTGTAGTGATGTAATGAATAAGAACAAAGTATAGTTTGTAATTTGATTCAAATCTAAAGTCTAAAGAGATATAATTAATTAATGTTTGGATTCACATGACATGTTGGTTTTTACAATTTAACAGGTCAAGTACGAGAAAATGGAGAAAAATATTGAGAAATTCGGTTCCGACCCCAAAAATCTTCGTATGGTGACTGATAGACCTTCTCGGATCGAGGTAAATCCAATAAAGGACTAATAATCATTATATCCTCACAAATTACATTGAATAATTTAGTTTAATGAATAAAAATATGTTTTTGTTAATATATCTTATGTGTTAATATTCACACTAGTAGTTAACCATTTGTAATAAGTTGGTTAATTTTTTTTTTTTACTAAAGTGATTAACTATTTTTTAAATGTGGTCTAAGTAAATTGTAAACTGTAAGTGGTTAGTATATTTCAATTTTCTTTGACAACCGAATGTAAATTGTAAATTGTATCTGATTTGACAATACAAAATGTGCATTTCATAATTCCTCAACTAATTAACAAACTAACTTTTATTGTTGTTAAAGATTGGGAAAAAAAGAAGGTTTTATTTATAATGTCTATTCATATTTGTTTATTTTATTTTAGATTATAAATGAAGAACCAGAAATTCCACGTGTTGTTTACGTGTCTCGTGAAAGAAGGCCATCACTTCCTCACAAATTCAAAGGAGGTGCCCTCAATACATTGGTGTGTACTTTTGATTTTAATTTATGCTTTTCTTTATATAATAATTTTTTTTGTGGCACAATTAATAAATTTCACTCATTTTTTTATTGTGGTTGCAGCTAAGAGTCTCAGGTTTAATCAGCAATGGACCTTATGTACTTGCAGTGGATTGTGATATGTATTGCAATGATCCATCTTCAGCCAAACAAGCCATGTGTTTCTTTCTTGATCCAGAAACCTCTAAATATATTGCATTTGTTCAATTCCCTCAAATGTTTCACAACCTTAGCAAAAAAGACATTTATGATAATCAATCTAGGACTGCTTTTAAGGTAAATCAACAAAATATTAATTAAGGTTTTCATTTAATTTATGTTATTTTTGCAATTTCTTATTTGTTTTTTGTGTTTGATGATCATATAAATGATGGCATAATGCAATTTATTTCAGACAATGTGGCAAGGGATGGATGGATTGAGAGGTCCTGGTCTTTCTGGCACTGGTAATTACTTGAATAGAAGTGCATTGTTATTTGGAAGTCCAATTCAAAAAGGTACATATTTCCTAATTATTATTTTGCAATCATATAAATATATTATATATATAAATCCACACATTCATTTTTTGCAATCAGATTCTAATTACTATTTTCCACATTATTACTTGTCATGTAAATTATACTCAATGTCATAGAAAAAGTTAAAAAAAATAGTCTTGCTTGTAAATTCATATATACAATATGCTCACATCATATATAAATTTCTATTATATATTCTTACATCATTGTATAAAAATTCAAACCTCAAGTGTTCATCCGTCGATACTCTTTGAAACATGAAGTCTGTCTTAATCTTGATGACCCATTAGGATCCAACACGTCTAAACTTTTTGTAATAAAATAATAATCCTGAAGCTATTAAGAGTCGTTGAATGATGTAGGATCTGAAAAATGTTACTATAATTGTAGACATCTTCATTTTACAAGTTTATTTTGTAGGTTTTGATCACAACCAAATTTTAAGAGGAATAGTACATAACTAGGATATGAACAACACTACTATAAGAATAAAAATAAATGAAGGTTAAAGAAACTTTGACAGAGACTTGCTGATTAAGGTTGACCTTTAAGCTGCTAGTAGATAATTTTTGCTAGGTGTACCTAAAATTAGGTTTTTAGTTGTCTCACTCTTGAGCTGATCTAGGTAAAATCTCTTAGAACTTGTGGTTGGTGTGCTTGGACCTAGCATAACCTCATAAAACTGACTTGTGAGGAGAGGATTGCACTTATAAACAAAACATATTTTGTTCGATTTGTGACTCTTTTAACACACCCCTCATGACAAGAACTATTGAGCTTGGTTCGTGGACATAAATGGTGGATGGCTGATAGTAGATAGCCCAACAGATCTTAACTGAAGCTCTGATACCATCTTAGAATTTGTGATTAAGCCTAACATAACTACACAAAACTGACTTGTGAGGTGAAAATTGTCTTCACTTATAAACGGATCATATTTTGTCTAATGTAGGACTCTTTAACAAAATCAACCACATAAAAATTTGGACTATTTAATGATATGAGTGAAACTATCCACAACATTGAAGTTAGCATTTCTAGCTCACCAATATAAGAGTACTTTTCTTTTTATGATTGTAGATGACTATCTTCTTGATGCCCAAAACTACTTTGGCAAGTCTACCACATACATAGAATCACTCAAGGCCATCCGAGGACAACAAACCATCAAAAAGAATCTTTCAAAAGAGGAAATTTTGCGAGAAGCTCAAGTAGTTGCATCTTCTTCCTACGAATCAAACACACAATGGGGCACAGAAGTAAGAGTATTGATACATTTATCAAAATCTAGGGAGATACCAAGTAAATAGATATTTTAGTCCGTAAAATTGTAAAGATCGACAATATAATTTTTTAAATTAAGTCGCTCAAATTAAAAAATGTCAATCAATTTAGTTTTTTCCTCAAATTTTTGCACCAAAATTTTAAAAGTTTACACATAACATAACAAGGGTAGAACCTCTAAAATTGTTGTACAAAATTTGAGAAAATAACCGAATTGATTGATATTTTTTCAATTTAAGCGACTATATTACTATTTCGTAAATTGAGGAGGTTAACTTGACGACTCTTCCAAATTCAATGACTAAAATGCCTATTACTATCTATAAAACATGACTTCCATGTTGTCAAATGTGGAGTGGCAAAAGTTGCTCCCAAAAATATACCACATATAGTATTCAGTTGATCTTATATTAAATGTTTGTGTATATAATAAATGAATTGATGATTTTACTAAATGTAAATTTGAATTTTTATTCACAGATAGGATTCTCATATGGCATCTTACTAGAGAGTACTATCACTGGCTATCTTTTGCACTGTAGAGGATGGAAATCAGCTTACCTTTACCCAAAAACACCATGTTTCTTAGGGTGTGCACCAACTGATATCAAAGAAGGCATGCTTCAATTGGTGAAGTGGTTGTCTGAACTTTGCTTGTTTGCTGTCTCTAAATACAGCCCTTTTACTTATGGATTTTCAAGAATGTCTGCTATTCACAACTTCACTTATTGCTTCATGTCTATTTCATCCATATATGCTATTGGATTCATACTTTATGGCATTGTACCTCAAGTCTGCTTCTTGAAAGGAATATCTGTGTTTCCAAAGGTAAATTATATATTCAATTCTTTTTATGTCTATTTAAAATTGCGATTGTGGTTATGCTGCGAACGTTTACATTGTGGTAAAATGCGGCCGATGCAATTGTGTTTGCAAACCGCAATTTAAAACCATGAATTTTATTATGATTGGACCTGCTTGGTTTGAAAAAATAAGTTGTCAATTTATTATGATCACTTTTAATTGCAAAATTGTCTATGCTACAATGGACCATTGGGAAATTGGAGGTCGGGTGGCACGTAAGTAAAGATTGATCAATGATTGTTTTGTCATAATTGAAATTCGAATTCTCCTAAACGATTTGTCTTTAACTAGAGTTTATTAACCACTTGACCTCAATCACTTGGTTTTTACATGAAACGGTGAACCATTTATTGTTTCATATATAAGTTTAAAAAAAAAGAGAGAATTTTGCAATTAAAAGTGAAATTTGAAAAATAAAAATAGGGACAGATTTTTTAAATATTAAAGAAAACTTAGATTTTCTCTTTATATTTTTTATCATATACTCATATAGTAAGTAAATTTTTGTCCTGGTTTTATAATTTAACATGTTTTAATTTACTGTTGTTACAGGTCACAGACCCTTGGTTTGCAGTGTTTGCATTCTTATATGTTGCCACACAAATTCAACATTTGATAGAGGTCATTTCTGGTGATGGCTCAGTGTCAATGTGGTGGGATGAACAAAGAATTTGGATTCTGAAATCAGTTACTAGTTTGTTTGCAATGACAGAGGCAGTTAAGAAATGGTTAGGATTGAACAAGAAGAAATTCAACTTGTCAAACAAAGCACTTGACACAGATAAAGAGAAAATCAAGAAATATGAACAAGGTAGGTTTGATTTTCAAGGTGCAGCTTTGTACATGTCTCCAATGGTTGTGTTACTCATAGTGAACACTATTTGCTTCTTTGGTGGTTTATGGAGACTATTCAATACAAGAGATTTTGAAGACATGTTTGGTCAACTTTTCCTGGTTAGCTATGTAATGGCTCTTAGCTATCCAATTTTTGAGGGGATTATAACTATGAAAAGTAAGAGTGGGTAG
Protein sequence

>MS.gene53239.t1

MTLAELILSVLWFFNQAYRWRPVSRSVMIEKLPADEKLPGLDIFVCTIDPEKEPTVEVMNTVVSAIAMDYPSNKLSIYLSDDGASAVTLFGIKEATQFAKVWVPFCKKYGVKSRCPKVKYEKMEKNIEKFGSDPKNLRMVTDRPSRIEIINEEPEIPRVVYVSRERRPSLPHKFKGGALNTLLRVSGLISNGPYVLAVDCDMYCNDPSSAKQAMCFFLDPETSKYIAFVQFPQMFHNLSKKDIYDNQSRTAFKTMWQGMDGLRGPGLSGTGNYLNRSALLFGSPIQKDDYLLDAQNYFGKSTTYIESLKAIRGQQTIKKNLSKEEILREAQVVASSSYESNTQWGTEIGFSYGILLESTITGYLLHCRGWKSAYLYPKTPCFLGCAPTDIKEGMLQLVKWLSELCLFAVSKYSPFTYGFSRMSAIHNFTYCFMSISSIYAIGFILYGIVPQVCFLKGISVFPKVTDPWFAVFAFLYVATQIQHLIEVISGDGSVSMWWDEQRIWILKSVTSLFAMTEAVKKWLGLNKKKFNLSNKALDTDKEKIKKYEQGRFDFQGAALYMSPMVVLLIVNTICFFGGLWRLFNTRDFEDMFGQLFLVSYVMALSYPIFEGIITMKSKSG