Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene53341.t1 | XP_024628602.1 | 60 | 250 | 76 | 4 | 23 | 264 | 25 | 258 | 5.00E-72 | 281.2 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene53341.t1 | A0A396GDW0 | 54.9 | 257 | 81 | 5 | 1 | 256 | 1 | 223 | 4.4e-62 | 247.7 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene06044 | MS.gene53341 | 0.80062 | 1.32E-48 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene53341.t1 | MTR_8g019450 | 53.086 | 243 | 105 | 4 | 19 | 257 | 2 | 239 | 3.54e-77 | 234 |
| MS.gene53341.t1 | MTR_8g019440 | 61.194 | 201 | 62 | 2 | 65 | 264 | 1 | 186 | 1.47e-74 | 225 |
| MS.gene53341.t1 | MTR_8g019060 | 52.778 | 252 | 85 | 4 | 1 | 252 | 44 | 261 | 4.09e-74 | 233 |
| MS.gene53341.t1 | MTR_8g019060 | 52.525 | 198 | 76 | 4 | 47 | 242 | 262 | 443 | 5.22e-60 | 196 |
| MS.gene53341.t1 | MTR_8g018980 | 49.425 | 261 | 107 | 4 | 1 | 257 | 1 | 240 | 4.44e-74 | 226 |
| MS.gene53341.t1 | MTR_0470s0020 | 48.659 | 261 | 109 | 4 | 1 | 257 | 1 | 240 | 9.78e-74 | 225 |
| MS.gene53341.t1 | MTR_8g019050 | 48.659 | 261 | 109 | 4 | 1 | 257 | 1 | 240 | 9.78e-74 | 225 |
| MS.gene53341.t1 | MTR_0514s0010 | 48.485 | 264 | 105 | 5 | 1 | 257 | 3 | 242 | 8.35e-73 | 223 |
| MS.gene53341.t1 | MTR_8g019380 | 46.531 | 245 | 119 | 7 | 19 | 259 | 2 | 238 | 5.33e-59 | 189 |
| MS.gene53341.t1 | MTR_8g019370 | 53.933 | 178 | 59 | 3 | 83 | 256 | 1 | 159 | 8.77e-53 | 169 |
| MS.gene53341.t1 | MTR_8g019290 | 37.097 | 186 | 90 | 3 | 56 | 237 | 8 | 170 | 1.33e-37 | 130 |
| MS.gene53341.t1 | MTR_8g019260 | 39.130 | 184 | 87 | 3 | 56 | 237 | 8 | 168 | 1.30e-36 | 128 |
| MS.gene53341.t1 | MTR_8g019110 | 39.130 | 184 | 87 | 3 | 56 | 237 | 8 | 168 | 1.30e-36 | 128 |
| MS.gene53341.t1 | MTR_7g055630 | 40.314 | 191 | 85 | 4 | 56 | 241 | 6 | 172 | 9.95e-36 | 126 |
| MS.gene53341.t1 | MTR_0470s0010 | 39.521 | 167 | 85 | 2 | 59 | 225 | 13 | 163 | 2.20e-32 | 117 |
| MS.gene53341.t1 | MTR_8g019020 | 39.521 | 167 | 85 | 2 | 59 | 225 | 13 | 163 | 2.20e-32 | 117 |
| MS.gene53341.t1 | MTR_8g019280 | 43.382 | 136 | 55 | 2 | 80 | 215 | 2 | 115 | 1.59e-29 | 109 |
| MS.gene53341.t1 | MTR_1g069110 | 29.310 | 232 | 129 | 8 | 14 | 242 | 235 | 434 | 4.24e-23 | 97.8 |
| MS.gene53341.t1 | MTR_7g099570 | 31.016 | 187 | 106 | 5 | 57 | 242 | 424 | 588 | 6.71e-19 | 86.3 |
| MS.gene53341.t1 | MTR_7g087540 | 26.289 | 194 | 105 | 4 | 56 | 249 | 28 | 183 | 1.80e-13 | 67.4 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Find 58 sgRNAs with CRISPR-Local
Find 75 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| TCACACTGCGTATTATTATT+TGG | 0.053800 | 8.4:-73842626 | MS.gene53341:CDS |
| TTACAAAGTTGCTTAAAATT+TGG | 0.132850 | 8.4:-73842680 | MS.gene53341:CDS |
| AACAATCAAGTTGATATTTA+TGG | 0.139857 | 8.4:-73843326 | MS.gene53341:CDS |
| CCACTTGATTGTTCATTGTC+TGG | 0.190538 | 8.4:+73843264 | None:intergenic |
| CACACTGCGTATTATTATTT+GGG | 0.201281 | 8.4:-73842625 | MS.gene53341:CDS |
| TCTTTGCTTCCTCTTTGGTT+GGG | 0.300905 | 8.4:+73843237 | None:intergenic |
| TTCTTTGCTTCCTCTTTGGT+TGG | 0.308886 | 8.4:+73843236 | None:intergenic |
| CACTTGATTGTTCATTGTCT+GGG | 0.318928 | 8.4:+73843265 | None:intergenic |
| GCATCATCCCAAACAGGATC+TGG | 0.359385 | 8.4:+73842714 | None:intergenic |
| AGGAAGTGAAAATAGTCTTC+AGG | 0.378179 | 8.4:+73842903 | None:intergenic |
| TGGTTACCCCAGATCCTGTT+TGG | 0.380833 | 8.4:-73842722 | MS.gene53341:CDS |
| CTTTGCTTCCTCTTTGGTTG+GGG | 0.392387 | 8.4:+73843238 | None:intergenic |
| CTGGGTTTCCTCGTTGCAAT+TGG | 0.392981 | 8.4:+73843283 | None:intergenic |
| AATCAAGCGGGTGCAATCAT+TGG | 0.400699 | 8.4:+73842555 | None:intergenic |
| AGCAACAATAACATTTCAAA+TGG | 0.436892 | 8.4:-73843102 | MS.gene53341:CDS |
| GTTTCCTCGTTGCAATTGGT+TGG | 0.445215 | 8.4:+73843287 | None:intergenic |
| TGCCTTGGACATCACAAGAT+AGG | 0.445667 | 8.4:+73842973 | None:intergenic |
| GTTTGTCTCATGCTTGTGAC+TGG | 0.448339 | 8.4:-73842843 | MS.gene53341:CDS |
| GGTTACCCCAGATCCTGTTT+GGG | 0.449719 | 8.4:-73842721 | MS.gene53341:CDS |
| AACATATTGTGTTCCACAAA+AGG | 0.450780 | 8.4:+73842883 | None:intergenic |
| AAGAGGAAGCAAAGAAAAGT+TGG | 0.454102 | 8.4:-73843229 | MS.gene53341:CDS |
| ATAGACTGTCGTTTCTGCCT+TGG | 0.463428 | 8.4:+73842958 | None:intergenic |
| CAGAAACGACAGTCTATGTC+CGG | 0.464179 | 8.4:-73842952 | MS.gene53341:CDS |
| GGAAGTGAAAATAGTCTTCA+GGG | 0.466085 | 8.4:+73842904 | None:intergenic |
| CCCTCAGCATCATCCCAAAC+AGG | 0.472772 | 8.4:+73842708 | None:intergenic |
| TAGAAAACCTATGACTAAAA+CGG | 0.479284 | 8.4:+73842817 | None:intergenic |
| CATCATCCCAAACAGGATCT+GGG | 0.491065 | 8.4:+73842715 | None:intergenic |
| AAGTTGCTTAAAATTTGGAT+TGG | 0.496440 | 8.4:-73842675 | MS.gene53341:CDS |
| CAAATGGCTTTAAATCCACC+CGG | 0.505694 | 8.4:-73843086 | MS.gene53341:CDS |
| TTTAACTGACCTAACGCCCC+CGG | 0.509095 | 8.4:+73843067 | None:intergenic |
| AAAGAAAAGTTGGAGATCAA+AGG | 0.524853 | 8.4:-73843219 | MS.gene53341:CDS |
| TCCTGTTTGGGATGATGCTG+AGG | 0.534190 | 8.4:-73842709 | MS.gene53341:CDS |
| ACACTGCGTATTATTATTTG+GGG | 0.542002 | 8.4:-73842624 | MS.gene53341:CDS |
| GAATGTTGCTATTATACCCA+TGG | 0.561628 | 8.4:+73843127 | None:intergenic |
| TCATCAACAATGGTGGCACT+AGG | 0.562931 | 8.4:+73842999 | None:intergenic |
| AATCAAGTTGATATTTATGG+TGG | 0.565785 | 8.4:-73843323 | MS.gene53341:CDS |
| CCTGTTTGGGATGATGCTGA+GGG | 0.567207 | 8.4:-73842708 | MS.gene53341:CDS |
| ATAGTCTTCAGGGTATATAA+CGG | 0.568060 | 8.4:+73842914 | None:intergenic |
| GGATAATATGAGAGTGACCA+TGG | 0.573596 | 8.4:-73843144 | MS.gene53341:CDS |
| GCACTAGGTGGATTTGCGTA+AGG | 0.584119 | 8.4:+73843014 | None:intergenic |
| CCAGACAATGAACAATCAAG+TGG | 0.595978 | 8.4:-73843264 | MS.gene53341:CDS |
| CTCCTATCTTGTGATGTCCA+AGG | 0.599271 | 8.4:-73842975 | MS.gene53341:CDS |
| AGCGGGTGCAATCATTGGAG+TGG | 0.604232 | 8.4:+73842560 | None:intergenic |
| AGAAACGACAGTCTATGTCC+GGG | 0.619574 | 8.4:-73842951 | MS.gene53341:CDS |
| AAATGGCTTTAAATCCACCC+GGG | 0.620191 | 8.4:-73843085 | MS.gene53341:CDS |
| ATTGTACGATCAAATCAAGC+GGG | 0.626580 | 8.4:+73842543 | None:intergenic |
| TCAACAATGGTGGCACTAGG+TGG | 0.631239 | 8.4:+73843002 | None:intergenic |
| AATGGCTTTAAATCCACCCG+GGG | 0.636763 | 8.4:-73843084 | MS.gene53341:CDS |
| AGTGGAAACCCCAACCAAAG+AGG | 0.641455 | 8.4:-73843246 | MS.gene53341:CDS |
| ATCATCCCAAACAGGATCTG+GGG | 0.642014 | 8.4:+73842716 | None:intergenic |
| GATTGTACGATCAAATCAAG+CGG | 0.644287 | 8.4:+73842542 | None:intergenic |
| GATAATATGAGAGTGACCAT+GGG | 0.661624 | 8.4:-73843143 | MS.gene53341:CDS |
| TAGGAGATCATCAACAATGG+TGG | 0.670648 | 8.4:+73842992 | None:intergenic |
| ACGGCTAATACAGCTTCACC+CGG | 0.678625 | 8.4:+73842933 | None:intergenic |
| AAAGATGACGCAGATACACA+TGG | 0.690019 | 8.4:-73843047 | MS.gene53341:CDS |
| AGATAGGAGATCATCAACAA+TGG | 0.693010 | 8.4:+73842989 | None:intergenic |
| AACTCCAACCAATTGCAACG+AGG | 0.732500 | 8.4:-73843291 | MS.gene53341:CDS |
| ATGGCTTTAAATCCACCCGG+GGG | 0.793886 | 8.4:-73843083 | MS.gene53341:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | CATATAAAAACAAGGATAAA+TGG | - | chr8.4:73842717-73842736 | MS.gene53341:CDS | 20.0% |
| !! | TTACAAAGTTGCTTAAAATT+TGG | - | chr8.4:73843206-73843225 | MS.gene53341:CDS | 20.0% |
| !!! | AACAATCAAGTTGATATTTA+TGG | - | chr8.4:73842560-73842579 | MS.gene53341:CDS | 20.0% |
| ! | AAGTTGCTTAAAATTTGGAT+TGG | - | chr8.4:73843211-73843230 | MS.gene53341:CDS | 25.0% |
| ! | AGCAACAATAACATTTCAAA+TGG | - | chr8.4:73842784-73842803 | MS.gene53341:CDS | 25.0% |
| ! | TAAAAACAAGGATAAATGGT+TGG | - | chr8.4:73842721-73842740 | MS.gene53341:CDS | 25.0% |
| ! | TAGAAAACCTATGACTAAAA+CGG | + | chr8.4:73843072-73843091 | None:intergenic | 25.0% |
| ! | TTACAAAAATGCAGTATCAA+TGG | - | chr8.4:73843144-73843163 | MS.gene53341:CDS | 25.0% |
| ! | TTGGTTGACATATAAAAACA+AGG | - | chr8.4:73842709-73842728 | MS.gene53341:CDS | 25.0% |
| !!! | AATCAAGTTGATATTTATGG+TGG | - | chr8.4:73842563-73842582 | MS.gene53341:CDS | 25.0% |
| AAAGAAAAGTTGGAGATCAA+AGG | - | chr8.4:73842667-73842686 | MS.gene53341:CDS | 30.0% | |
| AACATATTGTGTTCCACAAA+AGG | + | chr8.4:73843006-73843025 | None:intergenic | 30.0% | |
| ACACTGCGTATTATTATTTG+GGG | - | chr8.4:73843262-73843281 | MS.gene53341:CDS | 30.0% | |
| ATAGTCTTCAGGGTATATAA+CGG | + | chr8.4:73842975-73842994 | None:intergenic | 30.0% | |
| CACACTGCGTATTATTATTT+GGG | - | chr8.4:73843261-73843280 | MS.gene53341:CDS | 30.0% | |
| TCACACTGCGTATTATTATT+TGG | - | chr8.4:73843260-73843279 | MS.gene53341:CDS | 30.0% | |
| ! | ACTATTTTCACTTCCTTTTG+TGG | - | chr8.4:73842990-73843009 | MS.gene53341:CDS | 30.0% |
| ! | TCTATGTGGCTTTTATCTAT+GGG | - | chr8.4:73843088-73843107 | MS.gene53341:CDS | 30.0% |
| ! | TTAGTCATAGGTTTTCTATG+TGG | - | chr8.4:73843074-73843093 | MS.gene53341:CDS | 30.0% |
| ! | TTCTATGTGGCTTTTATCTA+TGG | - | chr8.4:73843087-73843106 | MS.gene53341:CDS | 30.0% |
| AAGAGGAAGCAAAGAAAAGT+TGG | - | chr8.4:73842657-73842676 | MS.gene53341:CDS | 35.0% | |
| AGATAGGAGATCATCAACAA+TGG | + | chr8.4:73842900-73842919 | None:intergenic | 35.0% | |
| AGGAAGTGAAAATAGTCTTC+AGG | + | chr8.4:73842986-73843005 | None:intergenic | 35.0% | |
| CAAAAACCGTGAAGGTAAAA+AGG | - | chr8.4:73843300-73843319 | MS.gene53341:CDS | 35.0% | |
| CACTTGATTGTTCATTGTCT+GGG | + | chr8.4:73842624-73842643 | None:intergenic | 35.0% | |
| GATAATATGAGAGTGACCAT+GGG | - | chr8.4:73842743-73842762 | MS.gene53341:CDS | 35.0% | |
| GGAAGTGAAAATAGTCTTCA+GGG | + | chr8.4:73842985-73843004 | None:intergenic | 35.0% | |
| ! | ACTTTTCTTTGCTTCCTCTT+TGG | + | chr8.4:73842657-73842676 | None:intergenic | 35.0% |
| ! | CTATGTGGCTTTTATCTATG+GGG | - | chr8.4:73843089-73843108 | MS.gene53341:CDS | 35.0% |
| ! | GAATGTTGCTATTATACCCA+TGG | + | chr8.4:73842762-73842781 | None:intergenic | 35.0% |
| ! | TGGTTTCCTTTTTACCTTCA+CGG | + | chr8.4:73843309-73843328 | None:intergenic | 35.0% |
| ! | TTTTACCTTCACGGTTTTTG+AGG | + | chr8.4:73843300-73843319 | None:intergenic | 35.0% |
| !!! | AAAGGTTTTAGAACAAGCAG+TGG | - | chr8.4:73842685-73842704 | MS.gene53341:CDS | 35.0% |
| !!! | TTTTAGAACAAGCAGTGGAT+TGG | - | chr8.4:73842690-73842709 | MS.gene53341:CDS | 35.0% |
| AAAGATGACGCAGATACACA+TGG | - | chr8.4:73842839-73842858 | MS.gene53341:CDS | 40.0% | |
| CCACTTGATTGTTCATTGTC+TGG | + | chr8.4:73842625-73842644 | None:intergenic | 40.0% | |
| CCAGACAATGAACAATCAAG+TGG | - | chr8.4:73842622-73842641 | MS.gene53341:CDS | 40.0% | |
| GATTTCCTCAAAAACCGTGA+AGG | - | chr8.4:73843292-73843311 | MS.gene53341:CDS | 40.0% | |
| GGATAATATGAGAGTGACCA+TGG | - | chr8.4:73842742-73842761 | MS.gene53341:CDS | 40.0% | |
| TAGGAGATCATCAACAATGG+TGG | + | chr8.4:73842897-73842916 | None:intergenic | 40.0% | |
| TCTTTGCTTCCTCTTTGGTT+GGG | + | chr8.4:73842652-73842671 | None:intergenic | 40.0% | |
| TTCTTTGCTTCCTCTTTGGT+TGG | + | chr8.4:73842653-73842672 | None:intergenic | 40.0% | |
| !! | AAATGGCTTTAAATCCACCC+GGG | - | chr8.4:73842801-73842820 | MS.gene53341:CDS | 40.0% |
| !! | CAAATGGCTTTAAATCCACC+CGG | - | chr8.4:73842800-73842819 | MS.gene53341:CDS | 40.0% |
| !! | CTGGTATCCGTTTTAGTCAT+AGG | - | chr8.4:73843062-73843081 | MS.gene53341:CDS | 40.0% |
| AACTCCAACCAATTGCAACG+AGG | - | chr8.4:73842595-73842614 | MS.gene53341:CDS | 45.0% | |
| AGAAACGACAGTCTATGTCC+GGG | - | chr8.4:73842935-73842954 | MS.gene53341:CDS | 45.0% | |
| ATCATCCCAAACAGGATCTG+GGG | + | chr8.4:73843173-73843192 | None:intergenic | 45.0% | |
| CAGAAACGACAGTCTATGTC+CGG | - | chr8.4:73842934-73842953 | MS.gene53341:CDS | 45.0% | |
| CATCATCCCAAACAGGATCT+GGG | + | chr8.4:73843174-73843193 | None:intergenic | 45.0% | |
| CTCCTATCTTGTGATGTCCA+AGG | - | chr8.4:73842911-73842930 | MS.gene53341:CDS | 45.0% | |
| CTTTGCTTCCTCTTTGGTTG+GGG | + | chr8.4:73842651-73842670 | None:intergenic | 45.0% | |
| GTTTCCTCGTTGCAATTGGT+TGG | + | chr8.4:73842602-73842621 | None:intergenic | 45.0% | |
| GTTTGTCTCATGCTTGTGAC+TGG | - | chr8.4:73843043-73843062 | MS.gene53341:CDS | 45.0% | |
| TCATCAACAATGGTGGCACT+AGG | + | chr8.4:73842890-73842909 | None:intergenic | 45.0% | |
| TGCCTTGGACATCACAAGAT+AGG | + | chr8.4:73842916-73842935 | None:intergenic | 45.0% | |
| ! | ATAGACTGTCGTTTCTGCCT+TGG | + | chr8.4:73842931-73842950 | None:intergenic | 45.0% |
| !! | AATGGCTTTAAATCCACCCG+GGG | - | chr8.4:73842802-73842821 | MS.gene53341:CDS | 45.0% |
| ACGGCTAATACAGCTTCACC+CGG | + | chr8.4:73842956-73842975 | None:intergenic | 50.0% | |
| AGTGGAAACCCCAACCAAAG+AGG | - | chr8.4:73842640-73842659 | MS.gene53341:CDS | 50.0% | |
| CTGGGTTTCCTCGTTGCAAT+TGG | + | chr8.4:73842606-73842625 | None:intergenic | 50.0% | |
| GCATCATCCCAAACAGGATC+TGG | + | chr8.4:73843175-73843194 | None:intergenic | 50.0% | |
| GGTTACCCCAGATCCTGTTT+GGG | - | chr8.4:73843165-73843184 | MS.gene53341:CDS | 50.0% | |
| TCAACAATGGTGGCACTAGG+TGG | + | chr8.4:73842887-73842906 | None:intergenic | 50.0% | |
| TGGTTACCCCAGATCCTGTT+TGG | - | chr8.4:73843164-73843183 | MS.gene53341:CDS | 50.0% | |
| TTTAACTGACCTAACGCCCC+CGG | + | chr8.4:73842822-73842841 | None:intergenic | 50.0% | |
| ! | CCTGTTTGGGATGATGCTGA+GGG | - | chr8.4:73843178-73843197 | MS.gene53341:CDS | 50.0% |
| ! | GCACTAGGTGGATTTGCGTA+AGG | + | chr8.4:73842875-73842894 | None:intergenic | 50.0% |
| ! | TCCTGTTTGGGATGATGCTG+AGG | - | chr8.4:73843177-73843196 | MS.gene53341:CDS | 50.0% |
| !! | ATGGCTTTAAATCCACCCGG+GGG | - | chr8.4:73842803-73842822 | MS.gene53341:CDS | 50.0% |
| AGCGGGTGCAATCATTGGAG+TGG | + | chr8.4:73843329-73843348 | None:intergenic | 55.0% | |
| CCCTCAGCATCATCCCAAAC+AGG | + | chr8.4:73843181-73843200 | None:intergenic | 55.0% | |
| TTAACTGACCTAACGCCCCC+GGG | + | chr8.4:73842821-73842840 | None:intergenic | 55.0% | |
| !! | TAAATCCACCCGGGGGCGTT+AGG | - | chr8.4:73842810-73842829 | MS.gene53341:CDS | 60.0% |
| ACTGACCTAACGCCCCCGGG+TGG | + | chr8.4:73842818-73842837 | None:intergenic | 70.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr8.4 | gene | 73842557 | 73843351 | 73842557 | ID=MS.gene53341 |
| chr8.4 | mRNA | 73842557 | 73843351 | 73842557 | ID=MS.gene53341.t1;Parent=MS.gene53341 |
| chr8.4 | exon | 73842557 | 73843351 | 73842557 | ID=MS.gene53341.t1.exon1;Parent=MS.gene53341.t1 |
| chr8.4 | CDS | 73842557 | 73843351 | 73842557 | ID=cds.MS.gene53341.t1;Parent=MS.gene53341.t1 |
| Gene Sequence |
| Protein sequence |
