Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene06044.t1 | XP_024634111.1 | 84.7 | 111 | 17 | 0 | 1 | 111 | 1 | 111 | 4.30E-50 | 207.2 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene06044.t1 | A0A396I567 | 62.9 | 105 | 37 | 1 | 1 | 103 | 1 | 105 | 1.6e-30 | 141.7 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene051932 | MS.gene06044 | 0.896567 | 3.26E-76 | -1.69E-46 |
| MS.gene053491 | MS.gene06044 | 0.920417 | 1.31E-87 | -1.69E-46 |
| MS.gene06044 | MS.gene061245 | 0.873527 | 1.37E-67 | -1.69E-46 |
| MS.gene06044 | MS.gene073742 | 0.823056 | 1.70E-53 | -1.69E-46 |
| MS.gene06044 | MS.gene41006 | 0.849005 | 4.27E-60 | -1.69E-46 |
| MS.gene06044 | MS.gene42436 | 0.852233 | 5.28E-61 | -1.69E-46 |
| MS.gene06044 | MS.gene43238 | 0.811906 | 5.54E-51 | -1.69E-46 |
| MS.gene06044 | MS.gene46426 | 0.849639 | 2.84E-60 | -1.69E-46 |
| MS.gene06044 | MS.gene53341 | 0.80062 | 1.32E-48 | -1.69E-46 |
| MS.gene06044 | MS.gene55278 | 0.905459 | 4.20E-80 | -1.69E-46 |
| MS.gene06044 | MS.gene62748 | 0.802131 | 6.49E-49 | -1.69E-46 |
| MS.gene06044 | MS.gene66513 | 0.879319 | 1.37E-69 | -1.69E-46 |
| MS.gene06044 | MS.gene81140 | 0.835227 | 1.90E-56 | -1.69E-46 |
| MS.gene06044 | MS.gene81193 | 0.840306 | 9.41E-58 | -1.69E-46 |
| MS.gene06044 | MS.gene91022 | 0.80673 | 7.14E-50 | -1.69E-46 |
| MS.gene06044 | MS.gene92171 | 0.823111 | 1.65E-53 | -1.69E-46 |
| MS.gene06044 | MS.gene93845 | 0.918226 | 2.02E-86 | -1.69E-46 |
| MS.gene06044 | MS.gene96600 | 0.853977 | 1.67E-61 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene06044.t1 | MTR_8g007240 | 42.222 | 90 | 46 | 4 | 4 | 91 | 38 | 123 | 4.65e-13 | 62.4 |
| MS.gene06044.t1 | MTR_3g102970 | 45.714 | 70 | 34 | 3 | 17 | 84 | 49 | 116 | 1.03e-11 | 58.9 |
| MS.gene06044.t1 | MTR_1g073480 | 48.980 | 49 | 24 | 1 | 15 | 63 | 93 | 140 | 8.15e-11 | 58.2 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Find 28 sgRNAs with CRISPR-Local
Find 42 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| AAAATCCAAAGAGGAAATTT+TGG | 0.165439 | 4.1:-4866263 | MS.gene06044:CDS |
| CATCTCCAAAATTTCCTCTT+TGG | 0.214318 | 4.1:+4866258 | None:intergenic |
| TTTGGAGATGTCGAAACTTT+TGG | 0.226089 | 4.1:-4866245 | MS.gene06044:CDS |
| ACCATTGTAATTTGTTCATA+TGG | 0.234426 | 4.1:-4866131 | MS.gene06044:CDS |
| TATGTGCGTGAAAGTGTTTC+TGG | 0.277333 | 4.1:-4865853 | MS.gene06044:CDS |
| TTTGGTAAATTTCCTCCAAC+TGG | 0.376381 | 4.1:+4866333 | None:intergenic |
| CCATTGTAATTTGTTCATAT+GGG | 0.394671 | 4.1:-4866130 | MS.gene06044:CDS |
| CCCAACTACAGCCTTCTTTG+AGG | 0.420158 | 4.1:+4865975 | None:intergenic |
| TTAAGCATTGCTCTAACTCT+AGG | 0.453670 | 4.1:+4866090 | None:intergenic |
| GGTAGCAAAGCTTCAGCTGT+TGG | 0.480338 | 4.1:-4866360 | MS.gene06044:CDS |
| GTAGCAAAGCTTCAGCTGTT+GGG | 0.484076 | 4.1:-4866359 | MS.gene06044:CDS |
| TGAGGACACATCATTATTTG+AGG | 0.495657 | 4.1:+4865993 | None:intergenic |
| TCCTCAAAGAAGGCTGTAGT+TGG | 0.512316 | 4.1:-4865976 | MS.gene06044:CDS |
| TAATGATGTGTCCTCAAAGA+AGG | 0.517374 | 4.1:-4865986 | MS.gene06044:CDS |
| TCAGCTGTTGGGTATCCAGT+TGG | 0.521283 | 4.1:-4866348 | MS.gene06044:CDS |
| TTCCAGTACCTTTCAAAATG+TGG | 0.526356 | 4.1:-4866016 | MS.gene06044:CDS |
| GTAATCAGATGATGAAATTG+TGG | 0.528968 | 4.1:-4866302 | MS.gene06044:CDS |
| ATCTGATTACAACCACACTT+TGG | 0.542708 | 4.1:+4866315 | None:intergenic |
| GCTGTTGGGTATCCAGTTGG+AGG | 0.547492 | 4.1:-4866345 | MS.gene06044:CDS |
| CCTCAAAGAAGGCTGTAGTT+GGG | 0.553172 | 4.1:-4865975 | MS.gene06044:intron |
| TAATCAGATGATGAAATTGT+GGG | 0.555715 | 4.1:-4866301 | MS.gene06044:CDS |
| GGAGGAAATTTACCAAAGTG+TGG | 0.580720 | 4.1:-4866327 | MS.gene06044:CDS |
| CCCATATGAACAAATTACAA+TGG | 0.636074 | 4.1:+4866130 | None:intergenic |
| ATTGCTGCAAAGAACAGTGA+AGG | 0.636453 | 4.1:-4866063 | MS.gene06044:CDS |
| ATACAGATGAAAATCCAAAG+AGG | 0.650224 | 4.1:-4866272 | MS.gene06044:CDS |
| AAGAACAGTGAAGGTGATGG+TGG | 0.677445 | 4.1:-4866054 | MS.gene06044:CDS |
| GCAAAGAACAGTGAAGGTGA+TGG | 0.689878 | 4.1:-4866057 | MS.gene06044:CDS |
| ATTAGGGTTCCTGAAATGGG+GGG | 0.693811 | 4.1:-4866381 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | AATTGAATTTGGAAAAGAAT+TGG | - | chr4.1:4866324-4866343 | MS.gene06044:CDS | 20.0% |
| !! | ATTGAATTTGGAAAAGAATT+GGG | - | chr4.1:4866325-4866344 | MS.gene06044:CDS | 20.0% |
| !! | TCTGATTACTTAAAACAATT+TGG | - | chr4.1:4866277-4866296 | MS.gene06044:CDS | 20.0% |
| !!! | AAATCTGATTTGTATGTATT+TGG | - | chr4.1:4866001-4866020 | MS.gene06044:CDS | 20.0% |
| ! | AAGGAAATTGGAATTGAATT+TGG | - | chr4.1:4866313-4866332 | MS.gene06044:CDS | 25.0% |
| ! | TAATCAGATGATGAAATTGT+GGG | - | chr4.1:4865907-4865926 | MS.gene06044:intron | 25.0% |
| !! | AAAATCCAAAGAGGAAATTT+TGG | - | chr4.1:4865945-4865964 | MS.gene06044:intron | 25.0% |
| !!! | ACCATTGTAATTTGTTCATA+TGG | - | chr4.1:4866077-4866096 | MS.gene06044:CDS | 25.0% |
| !!! | CCATTGTAATTTGTTCATAT+GGG | - | chr4.1:4866078-4866097 | MS.gene06044:CDS | 25.0% |
| AAACAATTTGGCAAAGAGAT+TGG | - | chr4.1:4866289-4866308 | MS.gene06044:CDS | 30.0% | |
| AACAATTTGGCAAAGAGATT+GGG | - | chr4.1:4866290-4866309 | MS.gene06044:CDS | 30.0% | |
| ATACAGATGAAAATCCAAAG+AGG | - | chr4.1:4865936-4865955 | MS.gene06044:intron | 30.0% | |
| CCCATATGAACAAATTACAA+TGG | + | chr4.1:4866081-4866100 | None:intergenic | 30.0% | |
| GTAATCAGATGATGAAATTG+TGG | - | chr4.1:4865906-4865925 | MS.gene06044:intron | 30.0% | |
| AAAGAGATTGGGAAGGAAAT+TGG | - | chr4.1:4866301-4866320 | MS.gene06044:CDS | 35.0% | |
| ATCTGATTACAACCACACTT+TGG | + | chr4.1:4865896-4865915 | None:intergenic | 35.0% | |
| ATTTGGAAAAGAATTGGGCA+AGG | - | chr4.1:4866330-4866349 | MS.gene06044:CDS | 35.0% | |
| CATCTCCAAAATTTCCTCTT+TGG | + | chr4.1:4865953-4865972 | None:intergenic | 35.0% | |
| TAATGATGTGTCCTCAAAGA+AGG | - | chr4.1:4866222-4866241 | MS.gene06044:intron | 35.0% | |
| TGAGGACACATCATTATTTG+AGG | + | chr4.1:4866218-4866237 | None:intergenic | 35.0% | |
| TTCCAGTACCTTTCAAAATG+TGG | - | chr4.1:4866192-4866211 | MS.gene06044:intron | 35.0% | |
| TTTGGTAAATTTCCTCCAAC+TGG | + | chr4.1:4865878-4865897 | None:intergenic | 35.0% | |
| ! | TTAAGCATTGCTCTAACTCT+AGG | + | chr4.1:4866121-4866140 | None:intergenic | 35.0% |
| ! | TTGGAGATGTCGAAACTTTT+GGG | - | chr4.1:4865964-4865983 | MS.gene06044:intron | 35.0% |
| ! | TTTGGAGATGTCGAAACTTT+TGG | - | chr4.1:4865963-4865982 | MS.gene06044:intron | 35.0% |
| !! | ATTTGAGGCCACATTTTGAA+AGG | + | chr4.1:4866203-4866222 | None:intergenic | 35.0% |
| ATTGCTGCAAAGAACAGTGA+AGG | - | chr4.1:4866145-4866164 | MS.gene06044:intron | 40.0% | |
| ATTTGGCAAAGAGATTGGGA+AGG | - | chr4.1:4866294-4866313 | MS.gene06044:CDS | 40.0% | |
| GGAGGAAATTTACCAAAGTG+TGG | - | chr4.1:4865881-4865900 | MS.gene06044:intron | 40.0% | |
| !! | TATGTGCGTGAAAGTGTTTC+TGG | - | chr4.1:4866355-4866374 | MS.gene06044:CDS | 40.0% |
| !!! | GATGGTGGTTTTAGCAACAT+TGG | - | chr4.1:4866169-4866188 | MS.gene06044:intron | 40.0% |
| CCTCAAAGAAGGCTGTAGTT+GGG | - | chr4.1:4866233-4866252 | MS.gene06044:intron | 45.0% | |
| GCAAAGAACAGTGAAGGTGA+TGG | - | chr4.1:4866151-4866170 | MS.gene06044:intron | 45.0% | |
| GTAGCAAAGCTTCAGCTGTT+GGG | - | chr4.1:4865849-4865868 | MS.gene06044:CDS | 45.0% | |
| TCCTCAAAGAAGGCTGTAGT+TGG | - | chr4.1:4866232-4866251 | MS.gene06044:intron | 45.0% | |
| ! | AAGAACAGTGAAGGTGATGG+TGG | - | chr4.1:4866154-4866173 | MS.gene06044:intron | 45.0% |
| ! | GAGATGTCGAAACTTTTGGG+AGG | - | chr4.1:4865967-4865986 | MS.gene06044:intron | 45.0% |
| !! | GGCCACATTTTGAAAGGTAC+TGG | + | chr4.1:4866197-4866216 | None:intergenic | 45.0% |
| CCCAACTACAGCCTTCTTTG+AGG | + | chr4.1:4866236-4866255 | None:intergenic | 50.0% | |
| GGTAGCAAAGCTTCAGCTGT+TGG | - | chr4.1:4865848-4865867 | MS.gene06044:CDS | 50.0% | |
| TCAGCTGTTGGGTATCCAGT+TGG | - | chr4.1:4865860-4865879 | MS.gene06044:intron | 50.0% | |
| GCTGTTGGGTATCCAGTTGG+AGG | - | chr4.1:4865863-4865882 | MS.gene06044:intron | 55.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr4.1 | gene | 4865842 | 4866388 | 4865842 | ID=MS.gene06044 |
| chr4.1 | mRNA | 4865842 | 4866388 | 4865842 | ID=MS.gene06044.t1;Parent=MS.gene06044 |
| chr4.1 | exon | 4866242 | 4866388 | 4866242 | ID=MS.gene06044.t1.exon1;Parent=MS.gene06044.t1 |
| chr4.1 | CDS | 4866242 | 4866388 | 4866242 | ID=cds.MS.gene06044.t1;Parent=MS.gene06044.t1 |
| chr4.1 | exon | 4865976 | 4866160 | 4865976 | ID=MS.gene06044.t1.exon2;Parent=MS.gene06044.t1 |
| chr4.1 | CDS | 4865976 | 4866160 | 4865976 | ID=cds.MS.gene06044.t1;Parent=MS.gene06044.t1 |
| chr4.1 | exon | 4865842 | 4865878 | 4865842 | ID=MS.gene06044.t1.exon3;Parent=MS.gene06044.t1 |
| chr4.1 | CDS | 4865842 | 4865878 | 4865842 | ID=cds.MS.gene06044.t1;Parent=MS.gene06044.t1 |
| Gene Sequence |
| Protein sequence |
