AlfalfaGEDB | MS.gene53369

M. sativa cultivar XinJiangDaYe / MS.gene53369

KEGG: -
GO: -
PFAM: -

Nr:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene53369.t1	XP_003598045.1	97.5	80	2	0	5	84	1	80	2.00E-35	157.9

Swissprot:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene53369.t1	Q9SF78	83.0	53	9	0	32	84	53	105	4.6e-20	98.2

Trembl:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene53369.t1	G7IYG4	97.5	80	2	0	5	84	1	80	1.5e-35	157.9

TFs/TRs:

Gene ID	Type	Classification

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network:

Gene1	Gene2	correlation coefficient	p_value	FDR
MS.gene050298	MS.gene53369	0.814445	1.53E-51	-1.69E-46
MS.gene050587	MS.gene53369	0.840611	7.83E-58	-1.69E-46
MS.gene05143	MS.gene53369	0.802506	5.43E-49	-1.69E-46
MS.gene05456	MS.gene53369	0.801874	7.33E-49	-1.69E-46

PPI:

Gene1	Gene2	Type

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene53369.t1	MTR_3g005650	97.500	80	2	0	5	84	1	80	3.25e-49	159
MS.gene53369.t1	MTR_0565s0010	64.286	70	25	0	15	84	13	82	4.77e-25	95.9
MS.gene53369.t1	MTR_3g024510	65.714	70	24	0	15	84	19	88	4.10e-24	93.2
MS.gene53369.t1	MTR_8g087890	60.494	81	28	2	7	84	1	80	1.02e-22	89.4
MS.gene53369.t1	MTR_3g005660	62.687	67	25	0	18	84	20	86	1.06e-22	89.4
MS.gene53369.t1	MTR_4g035353	51.899	79	33	1	11	84	3	81	4.20e-22	87.8
MS.gene53369.t1	MTR_2g017925	51.899	79	32	1	12	84	5	83	9.60e-20	81.3
MS.gene53369.t1	MTR_8g087870	54.667	75	31	2	13	84	5	79	1.14e-19	81.3
MS.gene53369.t1	MTR_2g017955	52.439	82	33	2	9	84	2	83	1.30e-19	81.3
MS.gene53369.t1	MTR_2g017915	54.430	79	30	2	12	84	5	83	1.74e-19	80.5
MS.gene53369.t1	MTR_2g033930	57.576	66	27	1	17	82	15	79	2.16e-19	80.5
MS.gene53369.t1	MTR_3g098270	54.217	83	31	2	7	82	3	85	1.58e-18	78.2
MS.gene53369.t1	MTR_3g023220	62.963	54	20	0	31	84	30	83	2.87e-18	77.4
MS.gene53369.t1	MTR_2g461580	50.667	75	37	0	10	84	7	81	5.63e-18	76.3
MS.gene53369.t1	MTR_2g033920	51.250	80	37	2	6	84	6	84	1.06e-17	75.5
MS.gene53369.t1	MTR_4g122200	57.746	71	28	2	14	84	16	84	1.28e-17	75.5
MS.gene53369.t1	MTR_2g033920	51.250	80	37	2	6	84	6	84	1.29e-17	75.5
MS.gene53369.t1	MTR_3g021460	53.623	69	31	1	17	84	13	81	1.42e-17	75.5
MS.gene53369.t1	MTR_2g017875	65.455	55	18	1	31	84	31	85	2.79e-17	74.7
MS.gene53369.t1	MTR_6g049220	69.231	52	15	1	32	82	28	79	3.35e-17	71.6
MS.gene53369.t1	MTR_5g084750	47.297	74	38	1	12	84	8	81	4.74e-17	73.9
MS.gene53369.t1	MTR_2g017965	50.617	81	38	2	6	84	4	84	5.07e-17	73.9
MS.gene53369.t1	MTR_1g025700	53.731	67	27	2	15	77	28	94	2.08e-16	72.4
MS.gene53369.t1	MTR_1g079530	51.250	80	37	2	7	84	1	80	1.00e-15	70.1
MS.gene53369.t1	MTR_8g075230	43.529	85	44	2	2	82	1	85	1.18e-15	70.1
MS.gene53369.t1	MTR_2g084585	53.731	67	29	2	18	82	13	79	1.97e-15	69.3
MS.gene53369.t1	MTR_0009s0250	44.595	74	37	2	13	82	9	82	2.48e-15	69.3
MS.gene53369.t1	MTR_7g116570	51.852	81	32	4	6	84	10	85	4.34e-15	68.6
MS.gene53369.t1	MTR_7g116520	50.000	72	34	2	15	84	14	85	4.53e-15	68.6
MS.gene53369.t1	MTR_4g056280	61.538	52	19	1	32	82	28	79	4.73e-15	68.6
MS.gene53369.t1	MTR_4g056160	52.778	72	29	3	13	82	8	76	5.89e-15	68.2
MS.gene53369.t1	MTR_3g114090	61.111	54	19	2	33	84	35	88	6.24e-15	68.2
MS.gene53369.t1	MTR_5g093090	54.286	70	30	2	17	84	11	80	8.66e-15	66.2
MS.gene53369.t1	MTR_6g021530	46.667	75	34	2	16	84	7	81	1.09e-14	67.4
MS.gene53369.t1	MTR_2g087292	43.836	73	37	2	13	81	9	81	1.58e-14	66.6
MS.gene53369.t1	MTR_2g018015	59.677	62	25	0	23	84	25	86	1.97e-14	66.2
MS.gene53369.t1	MTR_5g093090	54.286	70	30	2	17	84	11	80	2.60e-14	66.2
MS.gene53369.t1	MTR_7g116510	50.000	70	32	2	16	84	9	76	2.85e-14	66.2
MS.gene53369.t1	MTR_5g093090	54.286	70	30	2	17	84	11	80	3.12e-14	66.2
MS.gene53369.t1	MTR_1g025680	57.143	56	22	2	31	84	31	86	3.19e-14	66.2
MS.gene53369.t1	MTR_1g025780	60.417	48	18	1	31	77	38	85	3.43e-14	65.9
MS.gene53369.t1	MTR_4g019880	47.297	74	38	1	12	84	10	83	5.25e-14	65.5
MS.gene53369.t1	MTR_7g116500	45.946	74	39	1	12	84	11	84	6.07e-14	65.5
MS.gene53369.t1	MTR_4g036375	61.538	52	19	1	32	82	28	79	8.03e-14	63.5
MS.gene53369.t1	MTR_3g114120	54.545	55	24	1	31	84	44	98	1.01e-13	64.7
MS.gene53369.t1	MTR_3g082830	54.237	59	26	1	27	84	31	89	1.25e-13	64.3
MS.gene53369.t1	MTR_5g084770	46.377	69	34	2	12	77	4	72	1.59e-13	63.9
MS.gene53369.t1	MTR_1g025760	44.872	78	35	3	15	84	58	135	2.21e-13	63.9
MS.gene53369.t1	MTR_5g022640	50.877	57	26	1	30	84	39	95	2.58e-13	63.5
MS.gene53369.t1	MTR_1g025720	56.604	53	22	1	33	84	36	88	2.72e-13	63.5
MS.gene53369.t1	MTR_1g069175	48.649	74	32	2	12	84	16	84	5.54e-13	62.8
MS.gene53369.t1	MTR_3g464730	43.038	79	44	1	7	84	1	79	7.79e-13	62.0
MS.gene53369.t1	MTR_5g044210	62.500	40	15	0	32	71	67	106	1.06e-12	58.9
MS.gene53369.t1	MTR_8g079050	61.538	52	17	1	34	82	37	88	1.29e-12	61.6
MS.gene53369.t1	MTR_5g084790	47.826	69	34	2	17	84	16	83	1.60e-12	61.2
MS.gene53369.t1	MTR_2g015080	40.476	84	48	2	3	84	6	89	1.63e-12	61.2
MS.gene53369.t1	MTR_1g079590	52.632	57	26	1	29	84	26	82	2.64e-12	59.7
MS.gene53369.t1	MTR_1g079540	47.222	72	37	1	12	82	9	80	2.71e-12	60.8
MS.gene53369.t1	MTR_5g074270	54.000	50	22	1	34	82	33	82	3.05e-12	60.5
MS.gene53369.t1	MTR_3g052490	53.226	62	27	2	25	84	23	84	3.14e-12	60.5
MS.gene53369.t1	MTR_1g024890	54.386	57	25	1	29	84	27	83	3.21e-12	60.5
MS.gene53369.t1	MTR_8g014910	46.479	71	35	2	15	82	11	81	3.69e-12	60.5
MS.gene53369.t1	MTR_3g052580	42.500	80	44	2	7	84	1	80	5.88e-12	59.7
MS.gene53369.t1	MTR_3g052480	53.226	62	27	2	25	84	24	85	6.13e-12	59.7
MS.gene53369.t1	MTR_5g062890	56.604	53	21	2	32	82	26	78	1.90e-11	55.1
MS.gene53369.t1	MTR_3g052580	42.308	78	43	2	9	84	2	79	2.50e-11	58.2
MS.gene53369.t1	MTR_2g100620	55.769	52	22	1	32	82	29	80	4.02e-11	57.4
MS.gene53369.t1	MTR_8g075220	53.061	49	22	1	30	77	36	84	4.53e-11	57.4
MS.gene53369.t1	MTR_5g022630	53.448	58	24	2	30	84	39	96	4.76e-11	57.0
MS.gene53369.t1	MTR_8g075200	53.061	49	22	1	30	77	37	85	8.49e-11	56.6

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene53369.t1	AT1G71691	83.019	53	9	0	32	84	53	105	3.53e-25	95.5
MS.gene53369.t1	AT1G71691	83.019	53	9	0	32	84	53	105	6.09e-25	95.9
MS.gene53369.t1	AT5G45670	62.162	74	21	1	18	84	6	79	1.09e-22	89.4
MS.gene53369.t1	AT1G29670	55.128	78	34	1	7	84	5	81	6.42e-22	87.4
MS.gene53369.t1	AT1G29670	55.128	78	34	1	7	84	73	149	7.27e-22	87.8
MS.gene53369.t1	AT5G08460	51.613	93	36	3	1	84	8	100	1.05e-21	87.0
MS.gene53369.t1	AT2G19060	64.516	62	22	0	21	82	17	78	1.39e-21	86.3
MS.gene53369.t1	AT1G29660	53.947	76	34	1	7	82	5	79	1.72e-21	86.3
MS.gene53369.t1	AT4G18970	73.585	53	14	0	32	84	26	78	3.73e-21	85.1
MS.gene53369.t1	AT4G18970	73.585	53	14	0	32	84	26	78	5.31e-21	85.1
MS.gene53369.t1	AT4G16230	58.108	74	24	2	15	82	5	77	9.40e-21	82.8
MS.gene53369.t1	AT2G19010	49.398	83	42	0	2	84	28	110	9.86e-21	84.3
MS.gene53369.t1	AT2G19010	56.944	72	29	1	15	84	9	80	2.59e-20	82.8
MS.gene53369.t1	AT1G33811	68.519	54	17	0	31	84	31	84	7.69e-20	81.6
MS.gene53369.t1	AT4G16220	56.164	73	30	2	13	84	8	79	1.02e-19	79.7
MS.gene53369.t1	AT1G71250	58.462	65	26	1	19	82	25	89	1.02e-18	78.6
MS.gene53369.t1	AT5G15720	70.588	51	14	1	32	82	28	77	1.07e-18	78.2
MS.gene53369.t1	AT5G15720	70.588	51	14	1	32	82	28	77	1.37e-18	78.2
MS.gene53369.t1	AT2G19050	53.247	77	29	1	15	84	5	81	1.88e-18	77.8
MS.gene53369.t1	AT2G19050	53.247	77	29	1	15	84	5	81	2.03e-18	77.0
MS.gene53369.t1	AT4G30140	62.500	56	21	0	29	84	28	83	9.04e-18	75.9
MS.gene53369.t1	AT2G04020	59.649	57	22	1	29	84	38	94	3.14e-17	73.6
MS.gene53369.t1	AT2G04020	59.649	57	22	1	29	84	38	94	5.19e-17	73.6
MS.gene53369.t1	AT2G03980	57.895	57	23	1	29	84	38	94	6.90e-16	70.9
MS.gene53369.t1	AT2G03980	57.895	57	23	1	29	84	38	94	6.90e-16	70.9
MS.gene53369.t1	AT2G03980	57.895	57	23	1	29	84	38	94	6.90e-16	70.9
MS.gene53369.t1	AT2G03980	57.895	57	23	1	29	84	38	94	6.90e-16	70.9
MS.gene53369.t1	AT3G43570	48.750	80	37	3	9	84	2	81	1.72e-15	69.3
MS.gene53369.t1	AT3G43550	50.000	80	36	3	9	84	2	81	4.20e-15	68.2
MS.gene53369.t1	AT2G42990	45.833	72	38	1	7	77	1	72	4.50e-15	68.6
MS.gene53369.t1	AT5G33370	51.471	68	32	1	18	84	14	81	7.64e-15	67.8
MS.gene53369.t1	AT1G59406	52.778	72	30	3	17	84	10	81	8.65e-15	67.8
MS.gene53369.t1	AT1G59030	52.778	72	30	3	17	84	10	81	8.65e-15	67.8
MS.gene53369.t1	AT1G58725	52.778	72	30	3	17	84	10	81	8.65e-15	67.8
MS.gene53369.t1	AT5G33370	51.471	68	32	1	18	84	14	81	1.20e-14	67.4
MS.gene53369.t1	AT3G53100	47.436	78	39	2	7	82	1	78	1.21e-14	67.4
MS.gene53369.t1	AT1G75880	60.417	48	18	1	31	77	49	96	4.13e-14	65.9
MS.gene53369.t1	AT1G75880	60.417	48	18	1	31	77	49	96	4.33e-14	65.9
MS.gene53369.t1	AT5G18430	52.113	71	30	2	15	84	12	79	4.48e-14	65.9
MS.gene53369.t1	AT5G41890	55.224	67	26	2	19	82	17	82	8.90e-14	65.1
MS.gene53369.t1	AT3G14820	53.704	54	24	1	32	84	30	83	1.28e-13	64.3
MS.gene53369.t1	AT2G04570	44.595	74	38	3	12	82	4	77	1.53e-13	64.3
MS.gene53369.t1	AT1G20130	53.704	54	24	1	30	82	180	233	1.96e-13	63.9
MS.gene53369.t1	AT1G20132	55.357	56	24	1	30	84	57	112	1.98e-13	63.9
MS.gene53369.t1	AT3G50400	44.578	83	33	2	13	82	3	85	2.16e-13	63.9
MS.gene53369.t1	AT1G75920	48.333	60	30	1	26	84	21	80	2.20e-13	63.5
MS.gene53369.t1	AT1G75920	48.333	60	30	1	26	84	16	75	2.53e-13	63.5
MS.gene53369.t1	AT1G20130	53.704	54	24	1	30	82	200	253	2.53e-13	63.5
MS.gene53369.t1	AT1G75920	48.333	60	30	1	26	84	21	80	2.67e-13	63.5
MS.gene53369.t1	AT2G23540	47.500	80	30	2	15	82	20	99	4.11e-13	63.2
MS.gene53369.t1	AT3G04290	57.692	52	21	1	34	84	29	80	4.83e-13	62.8
MS.gene53369.t1	AT4G28780	57.692	52	21	1	34	84	31	82	4.93e-13	62.8
MS.gene53369.t1	AT4G10950	55.769	52	22	1	32	82	69	120	5.77e-13	62.8
MS.gene53369.t1	AT4G10950	55.769	52	22	1	32	82	69	120	5.92e-13	62.8
MS.gene53369.t1	AT5G22810	43.590	78	39	2	6	82	10	83	5.98e-13	62.4
MS.gene53369.t1	AT5G63170	46.667	75	38	2	12	84	5	79	7.78e-13	62.0
MS.gene53369.t1	AT1G58520	52.727	55	25	1	31	84	27	81	8.58e-13	62.0
MS.gene53369.t1	AT1G58525	52.727	55	25	1	31	84	27	81	8.58e-13	62.0
MS.gene53369.t1	AT5G55050	50.794	63	27	3	26	84	29	91	1.58e-12	61.2
MS.gene53369.t1	AT5G03810	46.154	78	33	3	14	82	2	79	4.09e-12	60.1
MS.gene53369.t1	AT4G26790	52.941	51	23	1	28	77	23	73	4.50e-12	60.1
MS.gene53369.t1	AT4G26790	52.941	51	23	1	28	77	23	73	4.50e-12	60.1
MS.gene53369.t1	AT5G45960	50.000	56	27	1	30	84	43	98	4.54e-12	60.1
MS.gene53369.t1	AT1G75930	48.529	68	33	2	17	82	11	78	4.57e-12	60.1
MS.gene53369.t1	AT1G53920	47.297	74	30	2	20	84	27	100	4.86e-12	60.1
MS.gene53369.t1	AT1G73610	54.545	55	24	1	31	84	32	86	8.36e-12	59.3
MS.gene53369.t1	AT5G03820	44.872	78	34	3	14	82	5	82	8.86e-12	59.3
MS.gene53369.t1	AT5G03820	44.872	78	34	3	14	82	33	110	9.55e-12	59.3
MS.gene53369.t1	AT1G71120	50.000	60	29	1	26	84	23	82	1.26e-11	58.9
MS.gene53369.t1	AT1G23500	47.619	84	40	2	2	84	6	86	2.22e-11	58.2
MS.gene53369.t1	AT1G75900	36.842	76	41	2	14	82	17	92	3.74e-11	57.4
MS.gene53369.t1	AT3G16370	52.727	55	25	1	29	82	25	79	4.29e-11	57.4
MS.gene53369.t1	AT1G20120	52.830	53	24	1	33	84	77	129	5.39e-11	57.0
MS.gene53369.t1	AT1G06990	53.226	62	25	2	27	84	27	88	6.27e-11	57.0

Find 20 sgRNAs with CRISPR-Local

Find 19 sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
TGAACATGGCAGGAACATTT+TGG	0.236523	3.2:-413530	None:intergenic
GTTCCTGCCATGTTCATATT+TGG	0.257412	3.2:+413537	MS.gene53369:CDS
GGTGACTCACTTATTGATAA+TGG	0.288022	3.2:+413558	MS.gene53369:CDS
TAGCCATCAACAAGTTTCTA+AGG	0.306918	3.2:-413446	None:intergenic
TGGAGTATCTCAAATATTCT+TGG	0.322971	3.2:+413473	MS.gene53369:CDS
AACTTGTTGATGGCTAAGTT+TGG	0.363571	3.2:+413453	MS.gene53369:CDS
CCATATGGTATTGACTTCAA+TGG	0.404544	3.2:+413624	MS.gene53369:CDS
AATATTCTTGGTGCTTATCA+TGG	0.407270	3.2:+413485	MS.gene53369:CDS
GAACCTTAGAAACTTGTTGA+TGG	0.410056	3.2:+413443	None:intergenic
TACACTATTGTTGATGAGAT+AGG	0.446947	3.2:+413675	MS.gene53369:CDS
CCGACTGGTCGCTTCTCAAA+TGG	0.461318	3.2:+413651	MS.gene53369:CDS
GACTTCAATGGTGGACCGAC+TGG	0.471274	3.2:+413636	MS.gene53369:CDS
GTGCTTATCATGGTCTTAAG+TGG	0.474885	3.2:+413495	MS.gene53369:CDS
CCATTGAAGTCAATACCATA+TGG	0.485315	3.2:-413624	None:intergenic
GTCTTAAGTGGTGCAGTCAC+AGG	0.491965	3.2:+413507	MS.gene53369:CDS
TCACCAAATATGAACATGGC+AGG	0.584345	3.2:-413540	None:intergenic
TATGGTATTGACTTCAATGG+TGG	0.612409	3.2:+413627	MS.gene53369:CDS
TGAGTCACCAAATATGAACA+TGG	0.664105	3.2:-413544	None:intergenic
TAATGGTAACAATAACAACA+TGG	0.669382	3.2:+413575	MS.gene53369:CDS
CCATTTGAGAAGCGACCAGT+CGG	0.684087	3.2:-413651	None:intergenic

CRISPR-GE

badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content
!	TAATGGTAACAATAACAACA+TGG	+	chr3.2:413575-413594	MS.gene53369:CDS	25.0%
!!	AAAGCTAACTATTTTCCATA+TGG	+	chr3.2:413609-413628	MS.gene53369:CDS	25.0%
!	TGGAGTATCTCAAATATTCT+TGG	+	chr3.2:413473-413492	MS.gene53369:CDS	30.0%
!!	AATATTCTTGGTGCTTATCA+TGG	+	chr3.2:413485-413504	MS.gene53369:CDS	30.0%
	CCATTGAAGTCAATACCATA+TGG	-	chr3.2:413627-413646	None:intergenic	35.0%
	GGTGACTCACTTATTGATAA+TGG	+	chr3.2:413558-413577	MS.gene53369:CDS	35.0%
	TAGCCATCAACAAGTTTCTA+AGG	-	chr3.2:413449-413468	None:intergenic	35.0%
	TGAGTCACCAAATATGAACA+TGG	-	chr3.2:413547-413566	None:intergenic	35.0%
!!	AACTTGTTGATGGCTAAGTT+TGG	+	chr3.2:413453-413472	MS.gene53369:CDS	35.0%
!!	CCATATGGTATTGACTTCAA+TGG	+	chr3.2:413624-413643	MS.gene53369:CDS	35.0%
!!	TATGGTATTGACTTCAATGG+TGG	+	chr3.2:413627-413646	MS.gene53369:CDS	35.0%
	GTTCCTGCCATGTTCATATT+TGG	+	chr3.2:413537-413556	MS.gene53369:CDS	40.0%
	TCACCAAATATGAACATGGC+AGG	-	chr3.2:413543-413562	None:intergenic	40.0%
!	GTGCTTATCATGGTCTTAAG+TGG	+	chr3.2:413495-413514	MS.gene53369:CDS	40.0%
!	TGAACATGGCAGGAACATTT+TGG	-	chr3.2:413533-413552	None:intergenic	40.0%
	CCATTTGAGAAGCGACCAGT+CGG	-	chr3.2:413654-413673	None:intergenic	50.0%
	GTCTTAAGTGGTGCAGTCAC+AGG	+	chr3.2:413507-413526	MS.gene53369:CDS	50.0%
!	CCGACTGGTCGCTTCTCAAA+TGG	+	chr3.2:413651-413670	MS.gene53369:CDS	55.0%
!!	GACTTCAATGGTGGACCGAC+TGG	+	chr3.2:413636-413655	MS.gene53369:CDS	55.0%

Chromosome	Type	Strat	End	Strand	Name
chr3.2	gene	413444	413696	413444	ID=MS.gene53369
chr3.2	mRNA	413444	413696	413444	ID=MS.gene53369.t1;Parent=MS.gene53369
chr3.2	exon	413444	413696	413444	ID=MS.gene53369.t1.exon1;Parent=MS.gene53369.t1
chr3.2	CDS	413444	413696	413444	ID=cds.MS.gene53369.t1;Parent=MS.gene53369.t1

Gene Sequence	>MS.gene53369 AACCTTAGAAACTTGTTGATGGCTAAGTTTGGAGTATCTCAAATATTCTTGGTGCTTATCATGGTCTTAAGTGGTGCAGTCACAGGCCAAAATGTTCCTGCCATGTTCATATTTGGTGACTCACTTATTGATAATGGTAACAATAACAACATGGCTTCATTAGCTAAAGCTAACTATTTTCCATATGGTATTGACTTCAATGGTGGACCGACTGGTCGCTTCTCAAATGGTTACACTATTGTTGATGAGATAG
Protein sequence	>MS.gene53369.t1 NLRNLLMAKFGVSQIFLVLIMVLSGAVTGQNVPAMFIFGDSLIDNGNNNNMASLAKANYFPYGIDFNGGPTGRFSNGYTIVDEI