AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene53377

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene53377.t1 MTR_8g087770 85.373 670 92 3 1 669 1 665 0.0 1152
MS.gene53377.t1 MTR_8g087750 82.406 665 112 3 6 669 11 671 0.0 1121
MS.gene53377.t1 MTR_8g087763 81.858 226 40 1 373 598 4 228 3.46e-131 391
MS.gene53377.t1 MTR_4g093750 33.666 401 241 10 66 451 35 425 8.08e-44 168
MS.gene53377.t1 MTR_4g093750 39.373 287 154 7 66 338 35 315 6.23e-43 166
MS.gene53377.t1 MTR_2g009690 38.246 285 164 6 62 336 40 322 3.56e-40 158
MS.gene53377.t1 MTR_5g009150 28.726 463 304 12 68 517 35 484 1.80e-34 139
MS.gene53377.t1 MTR_1g032550 34.062 320 179 10 68 368 35 341 3.08e-34 139
MS.gene53377.t1 MTR_8g105580 33.758 314 187 7 68 368 35 340 7.47e-34 137
MS.gene53377.t1 MTR_8g105580 33.758 314 187 7 68 368 35 340 9.93e-34 137
MS.gene53377.t1 MTR_8g009510 35.202 321 174 11 68 368 40 346 2.36e-33 136
MS.gene53377.t1 MTR_7g451010 34.688 320 177 10 68 368 40 346 1.52e-32 134
MS.gene53377.t1 MTR_4g098920 34.169 319 180 9 68 368 34 340 2.33e-31 130
MS.gene53377.t1 MTR_8g099550 32.803 314 191 7 68 368 34 340 5.08e-31 129
MS.gene53377.t1 MTR_8g027675 41.176 187 105 3 68 251 34 218 7.56e-31 128
MS.gene53377.t1 MTR_4g085860 73.684 76 19 1 205 279 2 77 6.33e-30 113
MS.gene53377.t1 MTR_6g045107 72.368 76 20 1 205 279 2 77 5.73e-28 107
MS.gene53377.t1 MTR_8g009510 31.474 251 142 8 135 368 2 239 1.99e-18 89.7
MS.gene53377.t1 MTR_8g463270 29.907 214 139 4 64 268 42 253 1.03e-16 84.7
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene53377.t1 AT1G60500 59.027 637 258 2 34 669 31 665 0.0 784
MS.gene53377.t1 AT1G60530 71.379 290 75 1 17 306 19 300 1.58e-142 419
MS.gene53377.t1 AT2G14120 29.505 444 290 11 24 451 1 437 7.21e-39 154
MS.gene53377.t1 AT2G14120 33.234 337 207 7 24 345 1 334 8.07e-39 154
MS.gene53377.t1 AT2G14120 29.505 444 290 11 24 451 1 437 8.88e-39 154
MS.gene53377.t1 AT2G14120 29.505 444 290 11 24 451 1 437 1.01e-38 154
MS.gene53377.t1 AT4G33650 29.705 441 268 11 35 451 30 452 2.08e-38 153
MS.gene53377.t1 AT4G33650 29.705 441 268 11 35 451 30 452 2.33e-38 152
MS.gene53377.t1 AT1G14830 33.439 314 189 7 68 368 35 341 7.22e-35 140
MS.gene53377.t1 AT5G42080 29.806 463 299 11 68 517 34 483 1.53e-34 140
MS.gene53377.t1 AT5G42080 29.806 463 299 11 68 517 34 483 1.53e-34 140
MS.gene53377.t1 AT3G60190 34.177 316 184 9 68 368 40 346 1.74e-33 137
MS.gene53377.t1 AT2G44590 24.682 628 398 23 68 671 35 611 7.40e-33 134
MS.gene53377.t1 AT5G42080 29.501 461 297 11 68 517 34 477 1.80e-32 133
MS.gene53377.t1 AT5G42080 33.548 310 194 6 68 368 34 340 1.91e-32 130
MS.gene53377.t1 AT3G61760 33.228 316 187 9 68 368 34 340 2.45e-30 125
MS.gene53377.t1 AT3G61760 33.228 316 187 9 68 368 34 340 2.88e-30 126
MS.gene53377.t1 AT3G61760 33.228 316 187 9 68 368 34 340 3.07e-30 127
MS.gene53377.t1 AT2G44590 23.567 628 388 24 68 671 35 594 1.22e-23 106
MS.gene53377.t1 AT2G44590 29.641 334 186 12 68 385 35 335 5.53e-23 104
MS.gene53377.t1 AT1G53140 27.848 237 157 6 43 268 36 269 1.09e-14 78.6

Find 131 sgRNAs with CRISPR-Local

Find 148 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TACAATAGATTTATCTATTT+TGG 0.051549 4.1:-13029439 None:intergenic
TTAATCTTCTTAATGATTTC+AGG 0.171207 4.1:-13029525 None:intergenic
GGAAAATTTGCCTGCCAATT+TGG 0.204709 4.1:+13029578 MS.gene53377:CDS
CTTGAGGAAGCCAAGATTAT+TGG 0.224459 4.1:+13029825 MS.gene53377:CDS
TTCTTAACAATCAAAGTTAA+AGG 0.234341 4.1:-13029018 None:intergenic
GCTGTTCTTGGCATGAAATT+TGG 0.254803 4.1:-13029852 None:intergenic
GAACTTTGATTTCGTCCATT+AGG 0.265918 4.1:-13029803 None:intergenic
GGGAAGTTGGATTCCCTCTT+TGG 0.273448 4.1:-13028734 None:intergenic
TGACAGTGTTTGGAACTATT+TGG 0.274905 4.1:+13029935 MS.gene53377:CDS
GAGGAGTCTCCTTCAATATC+AGG 0.303692 4.1:+13030464 MS.gene53377:CDS
TTACTACTTGTGAGTCCATT+AGG 0.307685 4.1:+13029205 MS.gene53377:CDS
GCCTGCCAATTTGGCATCAT+TGG 0.308754 4.1:+13029587 MS.gene53377:CDS
CAAAGCACCAGAAGGCTTGC+TGG 0.312662 4.1:+13029287 MS.gene53377:CDS
ATTTGATCATAGATATTATC+AGG 0.327362 4.1:-13029108 None:intergenic
TATTGTAGGAATTCCTGTTT+TGG 0.327596 4.1:+13029455 MS.gene53377:CDS
TTCAAGATAATACTTTCTTC+TGG 0.339329 4.1:-13029159 None:intergenic
ATCAGCCAATGATGCCAAAT+TGG 0.340224 4.1:-13029592 None:intergenic
AATGTAAGAAAAGCTGTTCT+TGG 0.343865 4.1:-13029864 None:intergenic
TAATATCTATGATCAAATTA+AGG 0.347385 4.1:+13029113 MS.gene53377:CDS
TTTGTGTAAGGAATAGAATT+GGG 0.352743 4.1:+13029352 MS.gene53377:CDS
TCTTAACAATCAAAGTTAAA+GGG 0.360721 4.1:-13029017 None:intergenic
AATGATTTCAGGCAAAGTTT+TGG 0.361226 4.1:-13029514 None:intergenic
TTAACCATGGTTGATCTTCC+AGG 0.367637 4.1:+13029057 MS.gene53377:CDS
CTTCCCACCATTGTTGTTGT+TGG 0.371021 4.1:+13028751 MS.gene53377:CDS
ACACTGTCAACAAAATTAAC+TGG 0.371215 4.1:-13029921 None:intergenic
TCACAAACTGTTGATAAAAC+TGG 0.375821 4.1:+13029231 MS.gene53377:CDS
GTTTGTGTAAGGAATAGAAT+TGG 0.375877 4.1:+13029351 MS.gene53377:CDS
AAAATAGATAAATCTATTGT+AGG 0.376484 4.1:+13029441 MS.gene53377:CDS
GACTCAGGCCTTTGACTTGA+AGG 0.382103 4.1:+13030280 MS.gene53377:CDS
ATTTGGAAGACCAATAATCT+TGG 0.383624 4.1:-13029835 None:intergenic
AGATCAACCATGGTTAAATC+TGG 0.385650 4.1:-13029051 None:intergenic
GGCTAATAGTTTACATGAGT+TGG 0.395161 4.1:+13029557 MS.gene53377:CDS
TCATTGTAAGATGATACTAT+TGG 0.397762 4.1:-13028658 None:intergenic
GTCTTGGTTATGTTTGTGTA+AGG 0.401697 4.1:+13029340 MS.gene53377:CDS
TGTCTCTTCCCTGATATTGA+AGG 0.410432 4.1:-13030473 None:intergenic
CATTACTTCAGAGGAATGTA+AGG 0.420635 4.1:+13029883 MS.gene53377:CDS
CAAGAGTACCATTGGTTATG+AGG 0.425077 4.1:+13028851 MS.gene53377:CDS
AACAGTTTGTGACATCCTAA+TGG 0.425745 4.1:-13029220 None:intergenic
ACAAGCTTCTGTGCCAAAAC+AGG 0.426561 4.1:-13029468 None:intergenic
ACAGCCACTGAGGAGCTTGC+TGG 0.430442 4.1:+13028970 MS.gene53377:CDS
TCAAGTACTGTGAATCTTGA+AGG 0.433218 4.1:+13030206 MS.gene53377:CDS
CAATGCATTTGCTTATCATC+AGG 0.438737 4.1:-13029699 None:intergenic
TAAGAAGATTAATGAGAAGT+TGG 0.440046 4.1:+13029536 MS.gene53377:CDS
CCTCAGCACAATCTTCCAAT+AGG 0.443004 4.1:-13030315 None:intergenic
GCATTGCACCGCTAGATTAG+TGG 0.443244 4.1:+13029716 MS.gene53377:CDS
ACCAGAAGGCTTGCTGGAAA+AGG 0.448222 4.1:+13029293 MS.gene53377:CDS
AGGCAAGGCTAATTGCCTAT+TGG 0.448827 4.1:+13030300 MS.gene53377:CDS
ATGTAACCCTGAGTACTTGC+AGG 0.450401 4.1:+13030118 MS.gene53377:CDS
ACAAGCTCTGGCTGTGGAAG+TGG 0.452099 4.1:-13028886 None:intergenic
TTATACTCCTGCAAGTACTC+AGG 0.459259 4.1:-13030125 None:intergenic
GGAAGAGACAGAAGTTGAGC+AGG 0.460748 4.1:+13030486 MS.gene53377:CDS
AGGGGTGTTGGAAATCCCTT+TGG 0.462637 4.1:-13028998 None:intergenic
CAACAACAATGGTGGGAAGT+TGG 0.467309 4.1:-13028747 None:intergenic
GATGATGTTAACATCGGTCT+TGG 0.467639 4.1:+13029324 MS.gene53377:CDS
AACAAAACAGACATGACAAA+AGG 0.469116 4.1:-13030593 None:intergenic
TTCGTCCATTAGGAAATCCT+TGG 0.470036 4.1:-13029793 None:intergenic
TTATCAGGTTGGCCATGTAC+AGG 0.471735 4.1:-13029093 None:intergenic
GAGCTTGCTGGCACTGCCAA+AGG 0.478600 4.1:+13028982 MS.gene53377:CDS
GTACTCTTGTGCAAATTCCT+TGG 0.481903 4.1:-13028837 None:intergenic
GCCAATGATGCCAAATTGGC+AGG 0.486397 4.1:-13029588 None:intergenic
TATGACCAAGGATTTCCTAA+TGG 0.487515 4.1:+13029788 MS.gene53377:CDS
TTATACTCTAAAACAAGCTC+TGG 0.488227 4.1:-13028898 None:intergenic
AATCAAAGTTAAAGGGGTGT+TGG 0.500893 4.1:-13029010 None:intergenic
TGTGCTGCAAGGTGTTGAAA+TGG 0.500919 4.1:+13030076 MS.gene53377:CDS
TGCAAACTATTATCAGCTGC+AGG 0.502339 4.1:+13029992 MS.gene53377:CDS
CTTGAATGTTCTTTCTGCCA+CGG 0.502379 4.1:+13029176 MS.gene53377:CDS
TCAGCATTTGTTGTTGACTC+AGG 0.505197 4.1:+13030265 MS.gene53377:CDS
ATTACTCGTGTCCCTGTACA+TGG 0.509421 4.1:+13029081 MS.gene53377:CDS
GTTGTTGGTGATCAATCGTC+TGG 0.509934 4.1:+13028766 MS.gene53377:CDS
GATCATAGATATTATCAGGT+TGG 0.512244 4.1:-13029104 None:intergenic
AATTTGCACAAGAGTACCAT+TGG 0.513388 4.1:+13028843 MS.gene53377:CDS
AGTGTCCTTGAGTCATTAGC+TGG 0.515596 4.1:+13028796 MS.gene53377:CDS
TCAAATTAAGGATATTATCA+TGG 0.518955 4.1:+13029125 MS.gene53377:CDS
ATATAATCATAGCTTGCACT+TGG 0.531392 4.1:-13029491 None:intergenic
AATGAACTGTTATCAGCAAA+TGG 0.537861 4.1:+13030419 MS.gene53377:CDS
ATAAGTTTGCCACGTGGCCA+AGG 0.541062 4.1:+13028820 MS.gene53377:CDS
TGAAGAATCATCACTTGCAT+TGG 0.541598 4.1:+13028591 MS.gene53377:CDS
AGCTTGCTGGCACTGCCAAA+GGG 0.547647 4.1:+13028983 MS.gene53377:CDS
CTTAACAATCAAAGTTAAAG+GGG 0.548616 4.1:-13029016 None:intergenic
TCTAAAACAAGCTCTGGCTG+TGG 0.552834 4.1:-13028892 None:intergenic
GGACGAAATCAAAGTTCTTG+AGG 0.554038 4.1:+13029809 MS.gene53377:CDS
AACTAAAACAAACATAATGT+TGG 0.557553 4.1:-13030546 None:intergenic
TAGCCTTGCCTTCAAGTCAA+AGG 0.560724 4.1:-13030288 None:intergenic
TGGCAATGTTGATACGCCGT+AGG 0.566250 4.1:-13028714 None:intergenic
ACAGGGACACGAGTAATGCC+TGG 0.569922 4.1:-13029075 None:intergenic
ATCACCAACAACAACAATGG+TGG 0.572674 4.1:-13028755 None:intergenic
AACAAACATAATGTTGGCCA+CGG 0.573159 4.1:-13030540 None:intergenic
CGTATCAACATTGCCAAAGA+GGG 0.575382 4.1:+13028721 MS.gene53377:CDS
AGTGCCAGCAAGCTCCTCAG+TGG 0.578957 4.1:-13028974 None:intergenic
TATACTCCTGCAAGTACTCA+GGG 0.580347 4.1:-13030124 None:intergenic
TGTGAATGTTGATCTGCAAA+AGG 0.585319 4.1:+13030388 MS.gene53377:CDS
AGGAGTCTCCTTCAATATCA+GGG 0.586223 4.1:+13030465 MS.gene53377:CDS
AGCTAATGACTCAAGGACAC+TGG 0.593764 4.1:-13028794 None:intergenic
ATTGGAAGATTGTGCTGAGG+AGG 0.597029 4.1:+13030318 MS.gene53377:CDS
ATTACTTCAGAGGAATGTAA+GGG 0.598482 4.1:+13029884 MS.gene53377:CDS
TTATACCAGCTAATGACTCA+AGG 0.606048 4.1:-13028801 None:intergenic
TATCAGGTTGGCCATGTACA+GGG 0.611782 4.1:-13029092 None:intergenic
CAAGCAAGTTTCAACTGATG+AGG 0.612510 4.1:+13028924 MS.gene53377:CDS
GCGTATCAACATTGCCAAAG+AGG 0.613041 4.1:+13028720 MS.gene53377:CDS
TCTATTCAACATGTGCTGCA+AGG 0.615822 4.1:+13030065 MS.gene53377:CDS
TAGATGCCCTAGAAAACCTA+CGG 0.617872 4.1:+13028698 MS.gene53377:CDS
TGGTGTTGAGAGGCTGCTTG+AGG 0.618613 4.1:+13030445 MS.gene53377:CDS
TCACCAACAACAACAATGGT+GGG 0.623546 4.1:-13028754 None:intergenic
AGGCCTTTGACTTGAAGGCA+AGG 0.625900 4.1:+13030285 MS.gene53377:CDS
CTGTTATCAGCAAATGGTGG+TGG 0.626367 4.1:+13030425 MS.gene53377:CDS
GTGCAAATTCCTTGGCCACG+TGG 0.626948 4.1:-13028829 None:intergenic
TGGTATTCCAGATTTAACCA+TGG 0.634354 4.1:+13029044 MS.gene53377:CDS
TTGATCACCAACAACAACAA+TGG 0.636905 4.1:-13028758 None:intergenic
CAAATGGTGGTGGTGTTGAG+AGG 0.637671 4.1:+13030435 MS.gene53377:CDS
TTGTGTAAGGAATAGAATTG+GGG 0.639370 4.1:+13029353 MS.gene53377:CDS
GCTCTGGCTGTGGAAGTGGA+TGG 0.648549 4.1:-13028882 None:intergenic
GGTGCAACAACAAGTGGTTG+AGG 0.648777 4.1:-13028637 None:intergenic
AAAGCTGACAAAGCACCAGA+AGG 0.649435 4.1:+13029279 MS.gene53377:CDS
AATTTGCAGAAAAGCAGACA+TGG 0.650037 4.1:-13029616 None:intergenic
TGCTGAAAGTAATATGACCA+AGG 0.653435 4.1:+13029776 MS.gene53377:CDS
GAGAGAAAGCAAAGAGACCG+TGG 0.654345 4.1:+13030523 MS.gene53377:CDS
CAACATATCCACTAATCTAG+CGG 0.654700 4.1:-13029724 None:intergenic
ACTATTGGTGCAACAACAAG+TGG 0.655043 4.1:-13028643 None:intergenic
TGCTATAAACACAGCCACTG+AGG 0.658388 4.1:+13028960 MS.gene53377:CDS
ACCGCTGATGATGTTAACAT+CGG 0.662734 4.1:+13029318 MS.gene53377:CDS
ACCGATGTTAACATCATCAG+CGG 0.666783 4.1:-13029319 None:intergenic
CTGTTGATAAAACTGGTCTG+AGG 0.690962 4.1:+13029238 MS.gene53377:CDS
TTACTTCAGAGGAATGTAAG+GGG 0.701485 4.1:+13029885 MS.gene53377:CDS
AATGCCTGGAAGATCAACCA+TGG 0.705380 4.1:-13029061 None:intergenic
CCTATTGGAAGATTGTGCTG+AGG 0.716472 4.1:+13030315 MS.gene53377:CDS
GTTGATCATCAACATCAACA+TGG 0.719635 4.1:-13028615 None:intergenic
ACAAGTAGTAAAATCAACCG+TGG 0.733510 4.1:-13029193 None:intergenic
GCTGATAATAGTTTGCAGAG+TGG 0.741343 4.1:-13029986 None:intergenic
GAACTGTTATCAGCAAATGG+TGG 0.747464 4.1:+13030422 MS.gene53377:CDS
GCTGGTATAAGTTTGCCACG+TGG 0.749894 4.1:+13028814 MS.gene53377:CDS
ACTGTGAATCTTGAAGGACA+TGG 0.765829 4.1:+13030212 MS.gene53377:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! AAAATAGATAAATCTATTGT+AGG + chr4.1:13029441-13029460 MS.gene53377:CDS 15.0%
!! TAATATCTATGATCAAATTA+AGG + chr4.1:13029113-13029132 MS.gene53377:CDS 15.0%
!!! TACAATAGATTTATCTATTT+TGG - chr4.1:13029442-13029461 None:intergenic 15.0%
!! AACTAAAACAAACATAATGT+TGG - chr4.1:13030549-13030568 None:intergenic 20.0%
!! ACTTTGATTGTTAAGAAAAA+TGG + chr4.1:13029024-13029043 MS.gene53377:CDS 20.0%
!! ATTTGATCATAGATATTATC+AGG - chr4.1:13029111-13029130 None:intergenic 20.0%
!! TCAAATTAAGGATATTATCA+TGG + chr4.1:13029125-13029144 MS.gene53377:CDS 20.0%
!! TCTTAACAATCAAAGTTAAA+GGG - chr4.1:13029020-13029039 None:intergenic 20.0%
!! TTAAAAAGATTCTTCTGATA+GGG + chr4.1:13029661-13029680 MS.gene53377:CDS 20.0%
!! TTAATCTTCTTAATGATTTC+AGG - chr4.1:13029528-13029547 None:intergenic 20.0%
!! TTCTTAACAATCAAAGTTAA+AGG - chr4.1:13029021-13029040 None:intergenic 20.0%
!!! AATAGAATTCTTTTTCTTCT+TGG - chr4.1:13030051-13030070 None:intergenic 20.0%
! CTTAAAAAGATTCTTCTGAT+AGG + chr4.1:13029660-13029679 MS.gene53377:CDS 25.0%
! CTTAACAATCAAAGTTAAAG+GGG - chr4.1:13029019-13029038 None:intergenic 25.0%
! TAAAAAGATTCTTCTGATAG+GGG + chr4.1:13029662-13029681 MS.gene53377:CDS 25.0%
! TAAGAAGATTAATGAGAAGT+TGG + chr4.1:13029536-13029555 MS.gene53377:CDS 25.0%
! TCATTGTAAGATGATACTAT+TGG - chr4.1:13028661-13028680 None:intergenic 25.0%
! TTCAAGATAATACTTTCTTC+TGG - chr4.1:13029162-13029181 None:intergenic 25.0%
! TTTGTGTAAGGAATAGAATT+GGG + chr4.1:13029352-13029371 MS.gene53377:CDS 25.0%
!! TTAATTTTGTTGACAGTGTT+TGG + chr4.1:13029925-13029944 MS.gene53377:CDS 25.0%
AACAAAACAGACATGACAAA+AGG - chr4.1:13030596-13030615 None:intergenic 30.0%
AATGAACTGTTATCAGCAAA+TGG + chr4.1:13030419-13030438 MS.gene53377:CDS 30.0%
AATGTAAGAAAAGCTGTTCT+TGG - chr4.1:13029867-13029886 None:intergenic 30.0%
ACACTGTCAACAAAATTAAC+TGG - chr4.1:13029924-13029943 None:intergenic 30.0%
ATTACTTCAGAGGAATGTAA+GGG + chr4.1:13029884-13029903 MS.gene53377:CDS 30.0%
ATTTGGAAGACCAATAATCT+TGG - chr4.1:13029838-13029857 None:intergenic 30.0%
GATCATAGATATTATCAGGT+TGG - chr4.1:13029107-13029126 None:intergenic 30.0%
GTTTGTGTAAGGAATAGAAT+TGG + chr4.1:13029351-13029370 MS.gene53377:CDS 30.0%
TTGTGTAAGGAATAGAATTG+GGG + chr4.1:13029353-13029372 MS.gene53377:CDS 30.0%
! AATGATTTCAGGCAAAGTTT+TGG - chr4.1:13029517-13029536 None:intergenic 30.0%
! ATATAATCATAGCTTGCACT+TGG - chr4.1:13029494-13029513 None:intergenic 30.0%
! CTTTTCTTACATTACTTCAG+AGG + chr4.1:13029874-13029893 MS.gene53377:CDS 30.0%
! TATTGTAGGAATTCCTGTTT+TGG + chr4.1:13029455-13029474 MS.gene53377:CDS 30.0%
! TCACAAACTGTTGATAAAAC+TGG + chr4.1:13029231-13029250 MS.gene53377:CDS 30.0%
! TTCACGTTTAGTAGATTTTG+TGG - chr4.1:13028575-13028594 None:intergenic 30.0%
!! TTATACTCTAAAACAAGCTC+TGG - chr4.1:13028901-13028920 None:intergenic 30.0%
!!! GAGCTTGTTTTAGAGTATAA+TGG + chr4.1:13028901-13028920 MS.gene53377:CDS 30.0%
AACAAACATAATGTTGGCCA+CGG - chr4.1:13030543-13030562 None:intergenic 35.0%
AACAGTTTGTGACATCCTAA+TGG - chr4.1:13029223-13029242 None:intergenic 35.0%
AATTTGCAGAAAAGCAGACA+TGG - chr4.1:13029619-13029638 None:intergenic 35.0%
ACAAGTAGTAAAATCAACCG+TGG - chr4.1:13029196-13029215 None:intergenic 35.0%
AGATCAACCATGGTTAAATC+TGG - chr4.1:13029054-13029073 None:intergenic 35.0%
CAACATATCCACTAATCTAG+CGG - chr4.1:13029727-13029746 None:intergenic 35.0%
CAATGCATTTGCTTATCATC+AGG - chr4.1:13029702-13029721 None:intergenic 35.0%
CATTACTTCAGAGGAATGTA+AGG + chr4.1:13029883-13029902 MS.gene53377:CDS 35.0%
GGCTAATAGTTTACATGAGT+TGG + chr4.1:13029557-13029576 MS.gene53377:CDS 35.0%
GTCTTGGTTATGTTTGTGTA+AGG + chr4.1:13029340-13029359 MS.gene53377:CDS 35.0%
GTTGATCATCAACATCAACA+TGG - chr4.1:13028618-13028637 None:intergenic 35.0%
TATGACCAAGGATTTCCTAA+TGG + chr4.1:13029788-13029807 MS.gene53377:CDS 35.0%
TGAAGAATCATCACTTGCAT+TGG + chr4.1:13028591-13028610 MS.gene53377:CDS 35.0%
TGCTGAAAGTAATATGACCA+AGG + chr4.1:13029776-13029795 MS.gene53377:CDS 35.0%
TGGTATTCCAGATTTAACCA+TGG + chr4.1:13029044-13029063 MS.gene53377:CDS 35.0%
TGTGAATGTTGATCTGCAAA+AGG + chr4.1:13030388-13030407 MS.gene53377:CDS 35.0%
TTACTACTTGTGAGTCCATT+AGG + chr4.1:13029205-13029224 MS.gene53377:CDS 35.0%
TTACTTCAGAGGAATGTAAG+GGG + chr4.1:13029885-13029904 MS.gene53377:CDS 35.0%
TTATACCAGCTAATGACTCA+AGG - chr4.1:13028804-13028823 None:intergenic 35.0%
TTGATCACCAACAACAACAA+TGG - chr4.1:13028761-13028780 None:intergenic 35.0%
! AATCAAAGTTAAAGGGGTGT+TGG - chr4.1:13029013-13029032 None:intergenic 35.0%
! GAACTTTGATTTCGTCCATT+AGG - chr4.1:13029806-13029825 None:intergenic 35.0%
! GAAGAAGAGAAGCTTTTTGA+GGG + chr4.1:13029402-13029421 MS.gene53377:CDS 35.0%
! TCAAGTACTGTGAATCTTGA+AGG + chr4.1:13030206-13030225 MS.gene53377:CDS 35.0%
! TGAAGAAGAGAAGCTTTTTG+AGG + chr4.1:13029401-13029420 MS.gene53377:CDS 35.0%
! TGACAGTGTTTGGAACTATT+TGG + chr4.1:13029935-13029954 MS.gene53377:CDS 35.0%
!! AATTTGCACAAGAGTACCAT+TGG + chr4.1:13028843-13028862 MS.gene53377:CDS 35.0%
!!! TGTCTGTTTTGTTTGTCTGT+TGG + chr4.1:13030603-13030622 MS.gene53377:CDS 35.0%
ACCGATGTTAACATCATCAG+CGG - chr4.1:13029322-13029341 None:intergenic 40.0%
ACTATTGGTGCAACAACAAG+TGG - chr4.1:13028646-13028665 None:intergenic 40.0%
ACTGTGAATCTTGAAGGACA+TGG + chr4.1:13030212-13030231 MS.gene53377:CDS 40.0%
AGGAGTCTCCTTCAATATCA+GGG + chr4.1:13030465-13030484 MS.gene53377:CDS 40.0%
ATCACCAACAACAACAATGG+TGG - chr4.1:13028758-13028777 None:intergenic 40.0%
ATCAGCCAATGATGCCAAAT+TGG - chr4.1:13029595-13029614 None:intergenic 40.0%
CAAGCAAGTTTCAACTGATG+AGG + chr4.1:13028924-13028943 MS.gene53377:CDS 40.0%
CGTATCAACATTGCCAAAGA+GGG + chr4.1:13028721-13028740 MS.gene53377:CDS 40.0%
CTTGAATGTTCTTTCTGCCA+CGG + chr4.1:13029176-13029195 MS.gene53377:CDS 40.0%
CTTGAGGAAGCCAAGATTAT+TGG + chr4.1:13029825-13029844 MS.gene53377:CDS 40.0%
GAACTGTTATCAGCAAATGG+TGG + chr4.1:13030422-13030441 MS.gene53377:CDS 40.0%
GATGATGTTAACATCGGTCT+TGG + chr4.1:13029324-13029343 MS.gene53377:CDS 40.0%
GCTGTTCTTGGCATGAAATT+TGG - chr4.1:13029855-13029874 None:intergenic 40.0%
GGAAAATTTGCCTGCCAATT+TGG + chr4.1:13029578-13029597 MS.gene53377:CDS 40.0%
GGACGAAATCAAAGTTCTTG+AGG + chr4.1:13029809-13029828 MS.gene53377:CDS 40.0%
GTACTCTTGTGCAAATTCCT+TGG - chr4.1:13028840-13028859 None:intergenic 40.0%
TAGATGCCCTAGAAAACCTA+CGG + chr4.1:13028698-13028717 MS.gene53377:CDS 40.0%
TATACTCCTGCAAGTACTCA+GGG - chr4.1:13030127-13030146 None:intergenic 40.0%
TCACCAACAACAACAATGGT+GGG - chr4.1:13028757-13028776 None:intergenic 40.0%
TCTATTCAACATGTGCTGCA+AGG + chr4.1:13030065-13030084 MS.gene53377:CDS 40.0%
TGCAAACTATTATCAGCTGC+AGG + chr4.1:13029992-13030011 MS.gene53377:CDS 40.0%
TGTCTCTTCCCTGATATTGA+AGG - chr4.1:13030476-13030495 None:intergenic 40.0%
TTAACCATGGTTGATCTTCC+AGG + chr4.1:13029057-13029076 MS.gene53377:CDS 40.0%
TTATACTCCTGCAAGTACTC+AGG - chr4.1:13030128-13030147 None:intergenic 40.0%
TTCGTCCATTAGGAAATCCT+TGG - chr4.1:13029796-13029815 None:intergenic 40.0%
! ACCGCTGATGATGTTAACAT+CGG + chr4.1:13029318-13029337 MS.gene53377:CDS 40.0%
! CTGTTGATAAAACTGGTCTG+AGG + chr4.1:13029238-13029257 MS.gene53377:CDS 40.0%
! GCTGATAATAGTTTGCAGAG+TGG - chr4.1:13029989-13030008 None:intergenic 40.0%
! TTTTGCAGCCTCATAACCAA+TGG - chr4.1:13028862-13028881 None:intergenic 40.0%
!! CAAGAGTACCATTGGTTATG+AGG + chr4.1:13028851-13028870 MS.gene53377:CDS 40.0%
!! TCAGCATTTGTTGTTGACTC+AGG + chr4.1:13030265-13030284 MS.gene53377:CDS 40.0%
!!! TTTTCTAGGGCATCTAGAAC+AGG - chr4.1:13028694-13028713 None:intergenic 40.0%
AATGCCTGGAAGATCAACCA+TGG - chr4.1:13029064-13029083 None:intergenic 45.0%
ACAAGCTTCTGTGCCAAAAC+AGG - chr4.1:13029471-13029490 None:intergenic 45.0%
AGCTAATGACTCAAGGACAC+TGG - chr4.1:13028797-13028816 None:intergenic 45.0%
AGGCAAGGCTAATTGCCTAT+TGG + chr4.1:13030300-13030319 MS.gene53377:CDS 45.0%
AGTGTCCTTGAGTCATTAGC+TGG + chr4.1:13028796-13028815 MS.gene53377:CDS 45.0%
ATGTAACCCTGAGTACTTGC+AGG + chr4.1:13030118-13030137 MS.gene53377:CDS 45.0%
ATTACTCGTGTCCCTGTACA+TGG + chr4.1:13029081-13029100 MS.gene53377:CDS 45.0%
ATTGGAAGATTGTGCTGAGG+AGG + chr4.1:13030318-13030337 MS.gene53377:CDS 45.0%
CCTATTGGAAGATTGTGCTG+AGG + chr4.1:13030315-13030334 MS.gene53377:CDS 45.0%
CCTCAGCACAATCTTCCAAT+AGG - chr4.1:13030318-13030337 None:intergenic 45.0%
CTGTTATCAGCAAATGGTGG+TGG + chr4.1:13030425-13030444 MS.gene53377:CDS 45.0%
GAGGAGTCTCCTTCAATATC+AGG + chr4.1:13030464-13030483 MS.gene53377:CDS 45.0%
GCGTATCAACATTGCCAAAG+AGG + chr4.1:13028720-13028739 MS.gene53377:CDS 45.0%
TGCTATAAACACAGCCACTG+AGG + chr4.1:13028960-13028979 MS.gene53377:CDS 45.0%
! AAAGCTGACAAAGCACCAGA+AGG + chr4.1:13029279-13029298 MS.gene53377:CDS 45.0%
! CAACAACAATGGTGGGAAGT+TGG - chr4.1:13028750-13028769 None:intergenic 45.0%
! CTTCCCACCATTGTTGTTGT+TGG + chr4.1:13028751-13028770 MS.gene53377:CDS 45.0%
! GATACGCCGTAGGTTTTCTA+GGG - chr4.1:13028707-13028726 None:intergenic 45.0%
! TAGCCTTGCCTTCAAGTCAA+AGG - chr4.1:13030291-13030310 None:intergenic 45.0%
! TCTAAAACAAGCTCTGGCTG+TGG - chr4.1:13028895-13028914 None:intergenic 45.0%
! TGATACGCCGTAGGTTTTCT+AGG - chr4.1:13028708-13028727 None:intergenic 45.0%
!! GTTGTTGGTGATCAATCGTC+TGG + chr4.1:13028766-13028785 MS.gene53377:CDS 45.0%
!! TATCAGGTTGGCCATGTACA+GGG - chr4.1:13029095-13029114 None:intergenic 45.0%
!! TGTGCTGCAAGGTGTTGAAA+TGG + chr4.1:13030076-13030095 MS.gene53377:CDS 45.0%
!! TTATCAGGTTGGCCATGTAC+AGG - chr4.1:13029096-13029115 None:intergenic 45.0%
ATAAGTTTGCCACGTGGCCA+AGG + chr4.1:13028820-13028839 MS.gene53377:CDS 50.0%
GAGAGAAAGCAAAGAGACCG+TGG + chr4.1:13030523-13030542 MS.gene53377:CDS 50.0%
GCATTGCACCGCTAGATTAG+TGG + chr4.1:13029716-13029735 MS.gene53377:CDS 50.0%
GCCAATGATGCCAAATTGGC+AGG - chr4.1:13029591-13029610 None:intergenic 50.0%
GCCTGCCAATTTGGCATCAT+TGG + chr4.1:13029587-13029606 MS.gene53377:CDS 50.0%
GCTGGTATAAGTTTGCCACG+TGG + chr4.1:13028814-13028833 MS.gene53377:CDS 50.0%
GGAAGAGACAGAAGTTGAGC+AGG + chr4.1:13030486-13030505 MS.gene53377:CDS 50.0%
GGGAAGTTGGATTCCCTCTT+TGG - chr4.1:13028737-13028756 None:intergenic 50.0%
GGTGCAACAACAAGTGGTTG+AGG - chr4.1:13028640-13028659 None:intergenic 50.0%
TGGCAATGTTGATACGCCGT+AGG - chr4.1:13028717-13028736 None:intergenic 50.0%
! ACCTTTTCCAGCAAGCCTTC+TGG - chr4.1:13029297-13029316 None:intergenic 50.0%
!! ACCAGAAGGCTTGCTGGAAA+AGG + chr4.1:13029293-13029312 MS.gene53377:CDS 50.0%
!! AGGCCTTTGACTTGAAGGCA+AGG + chr4.1:13030285-13030304 MS.gene53377:CDS 50.0%
!! AGGGGTGTTGGAAATCCCTT+TGG - chr4.1:13029001-13029020 None:intergenic 50.0%
!! CAAATGGTGGTGGTGTTGAG+AGG + chr4.1:13030435-13030454 MS.gene53377:CDS 50.0%
!! GACTCAGGCCTTTGACTTGA+AGG + chr4.1:13030280-13030299 MS.gene53377:CDS 50.0%
ACAGGGACACGAGTAATGCC+TGG - chr4.1:13029078-13029097 None:intergenic 55.0%
GTGCAAATTCCTTGGCCACG+TGG - chr4.1:13028832-13028851 None:intergenic 55.0%
! ACAAGCTCTGGCTGTGGAAG+TGG - chr4.1:13028889-13028908 None:intergenic 55.0%
! CAAAGCACCAGAAGGCTTGC+TGG + chr4.1:13029287-13029306 MS.gene53377:CDS 55.0%
! TGGTGTTGAGAGGCTGCTTG+AGG + chr4.1:13030445-13030464 MS.gene53377:CDS 55.0%
!! AGCTTGCTGGCACTGCCAAA+GGG + chr4.1:13028983-13029002 MS.gene53377:CDS 55.0%
ACAGCCACTGAGGAGCTTGC+TGG + chr4.1:13028970-13028989 MS.gene53377:CDS 60.0%
AGTGCCAGCAAGCTCCTCAG+TGG - chr4.1:13028977-13028996 None:intergenic 60.0%
GCTCTGGCTGTGGAAGTGGA+TGG - chr4.1:13028885-13028904 None:intergenic 60.0%
!! GAGCTTGCTGGCACTGCCAA+AGG + chr4.1:13028982-13029001 MS.gene53377:CDS 60.0%


Chromosome Type Strat End Strand Name
chr4.1 gene 13028550 13030634 13028550 ID=MS.gene53377
chr4.1 mRNA 13028550 13030634 13028550 ID=MS.gene53377.t1;Parent=MS.gene53377
chr4.1 exon 13028550 13030634 13028550 ID=MS.gene53377.t1.exon1;Parent=MS.gene53377.t1
chr4.1 CDS 13028550 13030634 13028550 ID=cds.MS.gene53377.t1;Parent=MS.gene53377.t1
Gene Sequence

>MS.gene53377

ATGGCTTTTGGAAAGAAGAGTTCCACAAAATCTACTAAACGTGAAGAATCATCACTTGCATTGGTCCATGTTGATGTTGATGATCAACCTCAACCACTTGTTGTTGCACCAATAGTATCATCTTACAATGAGAAGATACGTCCTGTTCTAGATGCCCTAGAAAACCTACGGCGTATCAACATTGCCAAAGAGGGAATCCAACTTCCCACCATTGTTGTTGTTGGTGATCAATCGTCTGGCAAGTCCAGTGTCCTTGAGTCATTAGCTGGTATAAGTTTGCCACGTGGCCAAGGAATTTGCACAAGAGTACCATTGGTTATGAGGCTGCAAAACCATCCACTTCCACAGCCAGAGCTTGTTTTAGAGTATAATGGCAAGCAAGTTTCAACTGATGAGGAAAATGTATCTGATGCTATAAACACAGCCACTGAGGAGCTTGCTGGCACTGCCAAAGGGATTTCCAACACCCCTTTAACTTTGATTGTTAAGAAAAATGGTATTCCAGATTTAACCATGGTTGATCTTCCAGGCATTACTCGTGTCCCTGTACATGGCCAACCTGATAATATCTATGATCAAATTAAGGATATTATCATGGAGTACATAACACCAGAAGAAAGTATTATCTTGAATGTTCTTTCTGCCACGGTTGATTTTACTACTTGTGAGTCCATTAGGATGTCACAAACTGTTGATAAAACTGGTCTGAGGACACTTGCTGTCGTAACAAAAGCTGACAAAGCACCAGAAGGCTTGCTGGAAAAGGTAACCGCTGATGATGTTAACATCGGTCTTGGTTATGTTTGTGTAAGGAATAGAATTGGGGATGAGTCTTATGAAGATGCTAGAAATGAAGAAGAGAAGCTTTTTGAGGGACATTCACTTCTTTCCAAAATAGATAAATCTATTGTAGGAATTCCTGTTTTGGCACAGAAGCTTGTCCAAGTGCAAGCTATGATTATATCCAAAACTTTGCCTGAAATCATTAAGAAGATTAATGAGAAGTTGGCTAATAGTTTACATGAGTTGGAAAATTTGCCTGCCAATTTGGCATCATTGGCTGATGCCATGTCTGCTTTTCTGCAAATTATCTCTTTGTCAAGAGACTCTCTTAAAAAGATTCTTCTGATAGGGGAGTTTGAAGAGTATCCTGATGATAAGCAAATGCATTGCACCGCTAGATTAGTGGATATGTTGAATTCATATGCAACTGAACTTCAAAACTGTGCTGAAAGTAATATGACCAAGGATTTCCTAATGGACGAAATCAAAGTTCTTGAGGAAGCCAAGATTATTGGTCTTCCAAATTTCATGCCAAGAACAGCTTTTCTTACATTACTTCAGAGGAATGTAAGGGGCGTTTCTTATTTGCCAGTTAATTTTGTTGACAGTGTTTGGAACTATTTGGAAACTGTTGTTACTTCTGTTTTGAATCGCCACTCTGCAAACTATTATCAGCTGCAGGTTTCAATTCGTAGAGCTGCTGAGCATCTTATTGCCAAGAAGAAAAAGAATTCTATTCAACATGTGCTGCAAGGTGTTGAAATGGAGAAGCTTACAGACTACACATGTAACCCTGAGTACTTGCAGGAGTATAACAAGCTGATGTCTAATCAAGAAGCGTTTTTGAAAGAAGTGTTGAATGCAAATAGAGAATCAAGTACTGTGAATCTTGAAGGACATGGTGAGATTGAAGTTGCTCATCTGAAGAACTATCAGCATTTGTTGTTGACTCAGGCCTTTGACTTGAAGGCAAGGCTAATTGCCTATTGGAAGATTGTGCTGAGGAGGCTCATTGATGTTATTGCTTTGCATTTGATGTTAAGCATCAATGAGCTTGTGAATGTTGATCTGCAAAAGGAGATCTGTAATGAACTGTTATCAGCAAATGGTGGTGGTGTTGAGAGGCTGCTTGAGGAGTCTCCTTCAATATCAGGGAAGAGACAGAAGTTGAGCAGGAGTGTGACAGTTTTGAGAGAAAGCAAAGAGACCGTGGCCAACATTATGTTTGTTTTAGTTTGTTTTTATCTATCTTGCAAGTGTCCTTTTGTCATGTCTGTTTTGTTTGTCTGTTGGAACTTTTAG
Protein sequence

>MS.gene53377.t1

MAFGKKSSTKSTKREESSLALVHVDVDDQPQPLVVAPIVSSYNEKIRPVLDALENLRRINIAKEGIQLPTIVVVGDQSSGKSSVLESLAGISLPRGQGICTRVPLVMRLQNHPLPQPELVLEYNGKQVSTDEENVSDAINTATEELAGTAKGISNTPLTLIVKKNGIPDLTMVDLPGITRVPVHGQPDNIYDQIKDIIMEYITPEESIILNVLSATVDFTTCESIRMSQTVDKTGLRTLAVVTKADKAPEGLLEKVTADDVNIGLGYVCVRNRIGDESYEDARNEEEKLFEGHSLLSKIDKSIVGIPVLAQKLVQVQAMIISKTLPEIIKKINEKLANSLHELENLPANLASLADAMSAFLQIISLSRDSLKKILLIGEFEEYPDDKQMHCTARLVDMLNSYATELQNCAESNMTKDFLMDEIKVLEEAKIIGLPNFMPRTAFLTLLQRNVRGVSYLPVNFVDSVWNYLETVVTSVLNRHSANYYQLQVSIRRAAEHLIAKKKKNSIQHVLQGVEMEKLTDYTCNPEYLQEYNKLMSNQEAFLKEVLNANRESSTVNLEGHGEIEVAHLKNYQHLLLTQAFDLKARLIAYWKIVLRRLIDVIALHLMLSINELVNVDLQKEICNELLSANGGGVERLLEESPSISGKRQKLSRSVTVLRESKETVANIMFVLVCFYLSCKCPFVMSVLFVCWNF