AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene53404

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene53404.t1 MTR_4g065077 94.780 364 19 0 1 364 1 364 0.0 727
MS.gene53404.t1 MTR_8g086470 90.385 364 19 1 1 364 17 364 0.0 684
MS.gene53404.t1 MTR_2g015920 46.575 365 180 8 2 357 1 359 1.36e-107 320
MS.gene53404.t1 MTR_2g016180 50.145 345 161 8 18 357 18 356 2.82e-106 321
MS.gene53404.t1 MTR_2g016270 49.855 345 147 8 18 357 18 341 3.12e-104 315
MS.gene53404.t1 MTR_2g015930 46.499 357 176 9 9 357 10 359 9.34e-102 305
MS.gene53404.t1 MTR_3g116080 41.292 356 181 14 14 357 9 348 1.25e-77 243
MS.gene53404.t1 MTR_4g094918 39.678 373 185 12 6 363 1 348 1.75e-76 240
MS.gene53404.t1 MTR_3g102220 41.484 364 186 13 10 357 10 362 2.23e-76 243
MS.gene53404.t1 MTR_3g112430 42.500 320 166 9 49 359 34 344 1.55e-75 240
MS.gene53404.t1 MTR_1g018840 39.628 376 193 14 2 357 1 362 4.69e-74 237
MS.gene53404.t1 MTR_3g075390 40.730 356 186 13 15 359 6 347 5.35e-74 234
MS.gene53404.t1 MTR_1g018840 39.628 376 193 14 2 357 1 362 6.19e-74 237
MS.gene53404.t1 MTR_4g079440 39.779 362 193 14 2 356 1 344 6.63e-74 233
MS.gene53404.t1 MTR_1g107550 42.308 338 171 13 32 357 31 356 2.58e-73 235
MS.gene53404.t1 MTR_2g090890 41.401 314 162 11 51 357 39 337 6.98e-70 222
MS.gene53404.t1 MTR_4g080700 40.063 317 164 11 49 355 38 338 1.20e-68 219
MS.gene53404.t1 MTR_4g080730 40.379 317 163 11 49 355 38 338 6.02e-68 217
MS.gene53404.t1 MTR_4g047610 38.994 318 179 8 45 355 32 341 6.63e-68 217
MS.gene53404.t1 MTR_4g104370 39.665 358 187 15 14 359 6 346 2.15e-67 216
MS.gene53404.t1 MTR_7g078600 40.575 313 166 9 49 356 40 337 5.01e-67 215
MS.gene53404.t1 MTR_5g022560 38.318 321 179 9 44 355 32 342 7.13e-66 212
MS.gene53404.t1 MTR_4g079470 36.957 322 187 8 41 355 31 343 9.15e-64 207
MS.gene53404.t1 MTR_4g079770 37.304 319 184 8 44 355 34 343 9.15e-64 207
MS.gene53404.t1 MTR_4g080360 36.512 367 182 14 9 356 7 341 1.42e-63 206
MS.gene53404.t1 MTR_4g107930 37.695 321 179 10 45 355 34 343 2.79e-63 206
MS.gene53404.t1 MTR_2g075830 35.457 361 186 11 11 355 10 339 2.67e-62 203
MS.gene53404.t1 MTR_7g079230 39.423 312 170 11 49 355 37 334 3.25e-62 202
MS.gene53404.t1 MTR_4g079800 36.760 321 183 9 44 355 34 343 1.60e-61 201
MS.gene53404.t1 MTR_2g083930 37.963 324 178 12 46 356 30 343 2.40e-60 198
MS.gene53404.t1 MTR_1g094135 32.888 374 211 11 6 352 2 362 7.01e-55 185
MS.gene53404.t1 MTR_1g023210 35.423 319 176 12 51 352 51 356 9.86e-55 184
MS.gene53404.t1 MTR_4g071060 33.155 374 204 13 3 352 1 352 1.74e-54 183
MS.gene53404.t1 MTR_2g015850 39.726 292 161 9 71 356 81 363 1.75e-53 181
MS.gene53404.t1 MTR_2g067900 36.533 323 169 10 52 354 17 323 7.78e-53 178
MS.gene53404.t1 MTR_2g067560 35.826 321 168 12 51 348 16 321 3.49e-50 180
MS.gene53404.t1 MTR_4g125300 31.492 362 226 8 3 356 1 348 6.30e-49 168
MS.gene53404.t1 MTR_2g064475 33.918 342 176 13 54 355 19 350 1.28e-47 165
MS.gene53404.t1 MTR_4g072790 31.232 349 190 11 14 352 8 316 4.06e-41 147
MS.gene53404.t1 MTR_4g073660 31.161 353 189 12 14 352 7 319 3.18e-40 145
MS.gene53404.t1 MTR_4g072340 33.010 309 164 10 51 352 48 320 3.25e-40 145
MS.gene53404.t1 MTR_1g023810 38.009 221 126 6 139 354 47 261 3.28e-40 143
MS.gene53404.t1 MTR_2g066980 32.602 319 160 14 54 355 37 317 3.99e-40 144
MS.gene53404.t1 MTR_7g078630 33.696 276 139 8 40 301 695 940 5.09e-35 136
MS.gene53404.t1 MTR_2g016380 45.378 119 60 3 241 357 21 136 3.70e-26 105
MS.gene53404.t1 MTR_2g015840 26.496 351 173 11 8 355 2 270 4.14e-26 105
MS.gene53404.t1 MTR_1g025450 39.655 174 79 7 139 306 53 206 5.74e-26 109
MS.gene53404.t1 MTR_7g078310 36.879 141 77 4 93 229 112 244 6.40e-22 94.0
MS.gene53404.t1 MTR_7g078580 44.954 109 50 6 250 356 1 101 1.26e-18 81.3
MS.gene53404.t1 MTR_3g029560 30.841 214 114 9 152 356 49 237 7.63e-18 82.4
MS.gene53404.t1 MTR_8g087510 30.709 127 84 3 182 306 23 147 2.68e-14 70.9
MS.gene53404.t1 MTR_7g079130 42.708 96 46 5 264 356 8 97 5.46e-14 67.8
MS.gene53404.t1 MTR_7g111050 26.033 242 139 9 141 352 100 331 5.14e-12 66.6
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene53404.t1 AT5G43060 41.944 360 180 12 13 357 9 354 2.80e-82 258
MS.gene53404.t1 AT4G23520 39.516 372 197 13 2 363 3 356 1.07e-80 251
MS.gene53404.t1 AT4G35350 41.111 360 192 12 6 358 8 354 2.54e-79 247
MS.gene53404.t1 AT1G20850 44.543 339 166 13 29 358 30 355 1.57e-78 245
MS.gene53404.t1 AT1G09850 43.252 326 157 10 45 358 26 335 2.51e-78 247
MS.gene53404.t1 AT1G47128 40.331 362 192 12 9 357 3 353 1.50e-76 243
MS.gene53404.t1 AT4G11310 39.178 365 192 13 13 362 9 358 6.74e-75 236
MS.gene53404.t1 AT5G50260 41.341 358 183 14 13 359 4 345 2.76e-74 234
MS.gene53404.t1 AT4G11320 37.197 371 210 11 4 362 6 365 8.90e-73 231
MS.gene53404.t1 AT4G11320 37.197 371 210 11 4 362 6 365 8.90e-73 231
MS.gene53404.t1 AT4G36880 40.000 350 186 12 25 357 20 362 6.80e-72 229
MS.gene53404.t1 AT3G48340 38.315 368 189 12 11 362 4 349 1.53e-70 225
MS.gene53404.t1 AT3G19400 40.483 331 176 13 43 363 36 355 1.03e-69 223
MS.gene53404.t1 AT3G19390 37.940 369 201 16 2 361 1 350 1.01e-68 223
MS.gene53404.t1 AT1G06260 37.394 353 197 10 12 357 8 343 4.45e-67 215
MS.gene53404.t1 AT3G48350 37.883 359 194 15 11 357 3 344 1.22e-66 215
MS.gene53404.t1 AT1G29090 39.335 361 182 14 13 356 14 354 4.17e-66 213
MS.gene53404.t1 AT2G34080 36.517 356 205 12 10 356 1 344 6.40e-66 212
MS.gene53404.t1 AT3G43960 40.244 328 178 12 46 364 36 354 7.51e-65 211
MS.gene53404.t1 AT5G45890 35.598 368 200 14 1 355 1 344 1.22e-64 209
MS.gene53404.t1 AT1G29080 36.134 357 205 12 9 356 3 345 3.60e-64 208
MS.gene53404.t1 AT3G49340 35.942 345 198 11 22 356 9 340 2.00e-63 206
MS.gene53404.t1 AT2G27420 37.363 364 194 15 10 356 1 347 1.08e-60 199
MS.gene53404.t1 AT4G35350 40.070 287 152 11 6 282 8 284 8.14e-57 187
MS.gene53404.t1 AT1G29110 33.894 357 191 13 15 356 7 333 9.50e-56 186
MS.gene53404.t1 AT3G54940 35.758 330 181 9 51 362 51 367 1.59e-55 186
MS.gene53404.t1 AT4G39090 34.234 333 183 11 51 363 51 367 1.06e-53 181
MS.gene53404.t1 AT5G60360 35.256 312 182 8 51 356 59 356 1.35e-52 178
MS.gene53404.t1 AT5G60360 36.934 287 167 7 51 331 59 337 1.98e-51 175
MS.gene53404.t1 AT5G60360 36.934 287 167 7 51 331 59 337 2.36e-51 175
MS.gene53404.t1 AT2G21430 35.974 303 164 11 51 333 48 340 2.85e-51 175
MS.gene53404.t1 AT4G16190 31.551 374 205 14 13 352 6 362 9.47e-50 171
MS.gene53404.t1 AT3G48350 35.254 295 169 11 11 294 3 286 1.88e-48 166
MS.gene53404.t1 AT3G45310 33.333 312 188 9 51 356 59 356 5.13e-48 166
MS.gene53404.t1 AT3G45310 35.192 287 172 8 51 331 59 337 2.26e-47 164
MS.gene53404.t1 AT3G19400 40.496 242 131 8 46 279 39 275 2.52e-47 162
MS.gene53404.t1 AT3G54940 36.139 202 116 3 51 243 51 248 7.34e-36 132
MS.gene53404.t1 AT4G01610 28.692 237 129 11 141 342 103 334 2.88e-12 67.8

Find 88 sgRNAs with CRISPR-Local

Find 121 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CTCATTGATATACTTAAAAT+TGG 0.205591 4.1:+13679924 None:intergenic
TATTGACAACCTTCTGGTTT+TGG 0.238326 4.1:-13679003 MS.gene53404:CDS
CCTATCAGCACAAGAAATTA+TGG 0.278118 4.1:-13679301 MS.gene53404:CDS
CGGTGAATGGTGAAGACTAT+TGG 0.304070 4.1:-13678509 MS.gene53404:CDS
TGCTCTAACCAACCACTAAA+TGG 0.307125 4.1:+13678344 None:intergenic
GTTATTGAAGATTGGCCTTA+TGG 0.314718 4.1:-13678423 MS.gene53404:CDS
TTTACTTTAATGCATTTGGC+TGG 0.318534 4.1:-13679242 MS.gene53404:CDS
TTTGGCTGGGTTATTGAAAA+TGG 0.328155 4.1:-13679228 MS.gene53404:CDS
CCATAATTTCTTGTGCTGAT+AGG 0.338269 4.1:+13679301 None:intergenic
CGTGCTCCTTCTTCCACAAT+TGG 0.339247 4.1:+13680004 None:intergenic
ACAACCTCTTAGTGTGAGTT+TGG 0.347332 4.1:-13678943 MS.gene53404:CDS
CATCTCTATCTTTGGTAAGT+AGG 0.351412 4.1:+13679816 None:intergenic
TGTTAGTATTGACAACCTTC+TGG 0.352494 4.1:-13679009 MS.gene53404:CDS
ACCTTCCCACTTCTGTAGAT+TGG 0.361626 4.1:-13679738 MS.gene53404:CDS
AAGGAGCTGTGACTGAAGTT+AGG 0.364375 4.1:-13679708 MS.gene53404:CDS
CTTTATCTTCAGCCATTTAG+TGG 0.368103 4.1:-13678356 None:intergenic
CATTGGGCATTCTCAGTCAC+TGG 0.368149 4.1:-13679369 MS.gene53404:CDS
AAAGGAATGTTATTGAAGAT+TGG 0.377330 4.1:-13678431 MS.gene53404:CDS
TTTAAATATCTCAAATCTCT+TGG 0.377666 4.1:+13679948 None:intergenic
TGACTAAAATAGGGAGTCTA+TGG 0.386085 4.1:-13678597 MS.gene53404:intron
TCTGTAGATTGGAGGGAAAA+AGG 0.392044 4.1:-13679727 MS.gene53404:CDS
AAACTCATGGGGAAAAGATT+GGG 0.397399 4.1:-13678478 MS.gene53404:CDS
GGAATCTGCATCTCTATCTT+TGG 0.403742 4.1:+13679808 None:intergenic
AAGGTTACTTGTTTATCAAA+AGG 0.408095 4.1:-13678449 MS.gene53404:CDS
GGACTGTGACCCTGCTAGTA+AGG 0.414218 4.1:-13679280 MS.gene53404:CDS
GGCAAATGAAAGACATGTAA+TGG 0.429471 4.1:+13680104 None:intergenic
TCAGTCACTGGGGCAATTGA+AGG 0.431508 4.1:-13679357 MS.gene53404:CDS
TCAAGGAATGCAAATCTTGT+TGG 0.438289 4.1:-13678552 MS.gene53404:CDS
AAAACTCATGGGGAAAAGAT+TGG 0.442756 4.1:-13678479 MS.gene53404:CDS
AAATCTTACGACAATTTCCT+TGG 0.443422 4.1:+13679683 None:intergenic
TTCCCTCCAATCGGGACAAT+GGG 0.454262 4.1:-13679789 MS.gene53404:CDS
TCTAATCGTGGGTTATGATT+CGG 0.463790 4.1:-13678529 MS.gene53404:CDS
AGGAGCTGTGACTGAAGTTA+GGG 0.480944 4.1:-13679707 MS.gene53404:CDS
TTACACCAGCATAGAATTGA+AGG 0.485435 4.1:+13678910 None:intergenic
AAGCTTCCCATTGTCCCGAT+TGG 0.485909 4.1:+13679783 None:intergenic
AGTGTGAGTTTGGATGCAAC+TGG 0.487456 4.1:-13678933 MS.gene53404:CDS
AGATGCAGATTCCCTCCAAT+CGG 0.487597 4.1:-13679798 MS.gene53404:CDS
CCTGCTAGTAAGGGTTGTGC+TGG 0.489644 4.1:-13679270 MS.gene53404:CDS
CTTGAAGGAAAATGCAAACA+AGG 0.490268 4.1:-13679033 MS.gene53404:intron
GCATCCAAACTCACACTAAG+AGG 0.491176 4.1:+13678939 None:intergenic
CCAGCACAACCCTTACTAGC+AGG 0.492421 4.1:+13679270 None:intergenic
TTACTTTAATGCATTTGGCT+GGG 0.503500 4.1:-13679241 MS.gene53404:CDS
GGCTGGGTTATTGAAAATGG+TGG 0.510307 4.1:-13679225 MS.gene53404:CDS
CTTCCCATTGTCCCGATTGG+AGG 0.510526 4.1:+13679786 None:intergenic
ATTCCCTCCAATCGGGACAA+TGG 0.514126 4.1:-13679790 MS.gene53404:CDS
ACTCTTCCAATTGTGGAAGA+AGG 0.517582 4.1:-13680010 MS.gene53404:CDS
GGAAAAGATTGGGGAGAGAA+AGG 0.518533 4.1:-13678468 MS.gene53404:CDS
GCTAGTAAGGGTTGTGCTGG+AGG 0.520226 4.1:-13679267 MS.gene53404:CDS
ACTGGCCTTCAATTCTATGC+TGG 0.521431 4.1:-13678915 MS.gene53404:CDS
TTCCCACTTCTGTAGATTGG+AGG 0.523180 4.1:-13679735 MS.gene53404:CDS
TTCCCTCCAATCTACAGAAG+TGG 0.526210 4.1:+13679732 None:intergenic
TCCCTCCAATCTACAGAAGT+GGG 0.527833 4.1:+13679733 None:intergenic
AAAGACATGTAATGGAAATG+AGG 0.531576 4.1:+13680112 None:intergenic
TGTTTGAACTCTTCCAATTG+TGG 0.531957 4.1:-13680017 MS.gene53404:CDS
TTGATTGCACATACACCATA+AGG 0.533070 4.1:+13678408 None:intergenic
GTGGGTTATGATTCGGTGAA+TGG 0.534697 4.1:-13678522 MS.gene53404:CDS
GATGCAGATTCCCTCCAATC+GGG 0.537566 4.1:-13679797 MS.gene53404:CDS
TTCCCATTGTCCCGATTGGA+GGG 0.544474 4.1:+13679787 None:intergenic
GCTGGGTTATTGAAAATGGT+GGG 0.551387 4.1:-13679224 MS.gene53404:CDS
AATCTTGTTGGTCTAATCGT+GGG 0.553988 4.1:-13678540 MS.gene53404:CDS
TCCCACTTCTGTAGATTGGA+GGG 0.556871 4.1:-13679734 MS.gene53404:CDS
TTCAAACACTTCTTCATCAG+AGG 0.560590 4.1:+13680032 None:intergenic
AATTTCTAGCCATGGCAAGT+TGG 0.565364 4.1:-13680064 MS.gene53404:CDS
GACTGTGACCCTGCTAGTAA+GGG 0.569928 4.1:-13679279 MS.gene53404:CDS
ATTGGGCATTCTCAGTCACT+GGG 0.573858 4.1:-13679368 MS.gene53404:CDS
AAATCTTGTTGGTCTAATCG+TGG 0.581439 4.1:-13678541 MS.gene53404:CDS
CAGAATGAACTTTGTGATGT+GGG 0.582146 4.1:+13680146 None:intergenic
GGACTAAACAAAATAGTCAC+GGG 0.593846 4.1:-13679336 MS.gene53404:CDS
AAAACTTGTCCAACTTGCCA+TGG 0.594424 4.1:+13680055 None:intergenic
GAGTACTCAATTTCTAGCCA+TGG 0.600592 4.1:-13680072 MS.gene53404:CDS
AATTGTGGAAGAAGGAGCAC+GGG 0.602772 4.1:-13680002 MS.gene53404:CDS
CTAAAATAGGGAGTCTATGG+TGG 0.604269 4.1:-13678594 MS.gene53404:intron
CAATTGTGGAAGAAGGAGCA+CGG 0.616767 4.1:-13680003 MS.gene53404:CDS
ACAGAATGAACTTTGTGATG+TGG 0.621850 4.1:+13680145 None:intergenic
AGGACTAAACAAAATAGTCA+CGG 0.622654 4.1:-13679337 MS.gene53404:CDS
GTGACTGAAGTTAGGGACCA+AGG 0.628455 4.1:-13679700 MS.gene53404:CDS
AACTCATGGGGAAAAGATTG+GGG 0.629466 4.1:-13678477 MS.gene53404:CDS
TCCAATCTACAGAAGTGGGA+AGG 0.633136 4.1:+13679737 None:intergenic
CAGCACAACCCTTACTAGCA+GGG 0.633326 4.1:+13679271 None:intergenic
CTGATCCATCCAAAACCAGA+AGG 0.636040 4.1:+13678994 None:intergenic
TGTGCAATCAATGCTGCGGT+TGG 0.646120 4.1:-13678396 MS.gene53404:CDS
CAGTGACTGAGAATGCCCAA+TGG 0.662774 4.1:+13679370 None:intergenic
AATTGAGTACTCACTAGACA+AGG 0.669581 4.1:+13680083 None:intergenic
AGACTATGCCAATTCAGAGG+AGG 0.671634 4.1:-13679977 MS.gene53404:CDS
GCGAGACTATGCCAATTCAG+AGG 0.673652 4.1:-13679980 MS.gene53404:CDS
GTTTGCTGATATGAGTCCTG+AGG 0.681956 4.1:-13679851 MS.gene53404:CDS
TTGGGCATTCTCAGTCACTG+GGG 0.757924 4.1:-13679367 MS.gene53404:CDS
TGTATGTGCAATCAATGCTG+CGG 0.774207 4.1:-13678400 MS.gene53404:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! AAAAAAAACATATAATTTGA+TGG + chr4.1:13679462-13679481 None:intergenic 10.0%
!! TTAAATTCATAAACATATAT+AGG + chr4.1:13678909-13678928 None:intergenic 10.0%
!!! AAATTTTTAAAACATTAACT+TGG - chr4.1:13679736-13679755 MS.gene53404:CDS 10.0%
!! TGAAAATTACTAATAAAGAA+TGG + chr4.1:13678871-13678890 None:intergenic 15.0%
!!! AATATACAAGTGAATTTTTA+AGG - chr4.1:13679017-13679036 MS.gene53404:CDS 15.0%
!!! TAATAATCCATATATTTTCT+TGG - chr4.1:13679088-13679107 MS.gene53404:intron 15.0%
!!! TTATATGTTTTTTTTCTTGA+AGG - chr4.1:13679467-13679486 MS.gene53404:intron 15.0%
!! AATAAGTCCAAGAAAATATA+TGG + chr4.1:13679098-13679117 None:intergenic 20.0%
!! CTCATTGATATACTTAAAAT+TGG + chr4.1:13678594-13678613 None:intergenic 20.0%
!! TTAGAAAAGACAAATAGTTA+AGG + chr4.1:13679394-13679413 None:intergenic 20.0%
!! TTCCTATATATACTTTCTAA+AGG + chr4.1:13678942-13678961 None:intergenic 20.0%
!! TTTAAATATCTCAAATCTCT+TGG + chr4.1:13678570-13678589 None:intergenic 20.0%
!!! ATTGTTGTTTTGACTAAAAT+AGG - chr4.1:13679908-13679927 MS.gene53404:CDS 20.0%
!!! TACCTTTAGAAAGTATATAT+AGG - chr4.1:13678937-13678956 MS.gene53404:CDS 20.0%
!!! TATCCTTATTTAGCTAAAAA+TGG - chr4.1:13679332-13679351 MS.gene53404:CDS 20.0%
!!! TGCACTTAAGAAATTTTTTT+AGG - chr4.1:13679761-13679780 MS.gene53404:CDS 20.0%
!!! TTGTTGTTTTGACTAAAATA+GGG - chr4.1:13679909-13679928 MS.gene53404:CDS 20.0%
! AAAGGAATGTTATTGAAGAT+TGG - chr4.1:13680084-13680103 MS.gene53404:CDS 25.0%
! AAGGTTACTTGTTTATCAAA+AGG - chr4.1:13680066-13680085 MS.gene53404:CDS 25.0%
! TTTGACTATTGAAAGTCAAT+TGG + chr4.1:13679719-13679738 None:intergenic 25.0%
!! AAAGTCAATTGGTTTAACTA+TGG + chr4.1:13679708-13679727 None:intergenic 25.0%
!! GGTTTTTACTTTAATGCATT+TGG - chr4.1:13679269-13679288 MS.gene53404:CDS 25.0%
!!! AAGATAAAGCTGTTTTGATT+GGG + chr4.1:13680148-13680167 None:intergenic 25.0%
!!! GTACCATTTTTAGCTAAATA+AGG + chr4.1:13679338-13679357 None:intergenic 25.0%
AAATCTTACGACAATTTCCT+TGG + chr4.1:13678835-13678854 None:intergenic 30.0%
AGAACTGCAAAAAAGATTCA+AGG - chr4.1:13679946-13679965 MS.gene53404:CDS 30.0%
AGGACTAAACAAAATAGTCA+CGG - chr4.1:13679178-13679197 MS.gene53404:CDS 30.0%
ATCTTTCAAAAGGTTGAATC+TGG - chr4.1:13679817-13679836 MS.gene53404:CDS 30.0%
ATTGGATTGTGAAAAACTCA+TGG - chr4.1:13680024-13680043 MS.gene53404:CDS 30.0%
TTACTTTAATGCATTTGGCT+GGG - chr4.1:13679274-13679293 MS.gene53404:CDS 30.0%
TTGGATTGTGAAAAACTCAT+GGG - chr4.1:13680025-13680044 MS.gene53404:CDS 30.0%
TTTACTTTAATGCATTTGGC+TGG - chr4.1:13679273-13679292 MS.gene53404:CDS 30.0%
! AAAGACATGTAATGGAAATG+AGG + chr4.1:13678406-13678425 None:intergenic 30.0%
! GTGTCGATATATCTTTCAAA+AGG - chr4.1:13679807-13679826 MS.gene53404:CDS 30.0%
!! TTATTTTCTAGAGAGCCATT+GGG - chr4.1:13679130-13679149 MS.gene53404:intron 30.0%
!!! GAAGATAAAGCTGTTTTGAT+TGG + chr4.1:13680149-13680168 None:intergenic 30.0%
AAAACTCATGGGGAAAAGAT+TGG - chr4.1:13680036-13680055 MS.gene53404:CDS 35.0%
AAACTCATGGGGAAAAGATT+GGG - chr4.1:13680037-13680056 MS.gene53404:CDS 35.0%
AATTGAGTACTCACTAGACA+AGG + chr4.1:13678435-13678454 None:intergenic 35.0%
ACAGAATGAACTTTGTGATG+TGG + chr4.1:13678373-13678392 None:intergenic 35.0%
AGCTAAAAATGGTACTTGCA+AGG - chr4.1:13679343-13679362 MS.gene53404:CDS 35.0%
CAGAATGAACTTTGTGATGT+GGG + chr4.1:13678372-13678391 None:intergenic 35.0%
CATCTCTATCTTTGGTAAGT+AGG + chr4.1:13678702-13678721 None:intergenic 35.0%
CCTATCAGCACAAGAAATTA+TGG - chr4.1:13679214-13679233 MS.gene53404:CDS 35.0%
CTTGAAGGAAAATGCAAACA+AGG - chr4.1:13679482-13679501 MS.gene53404:intron 35.0%
GGACTAAACAAAATAGTCAC+GGG - chr4.1:13679179-13679198 MS.gene53404:CDS 35.0%
GGCAAATGAAAGACATGTAA+TGG + chr4.1:13678414-13678433 None:intergenic 35.0%
TAAAGTTTAGTGCTAATGCG+AGG - chr4.1:13679785-13679804 MS.gene53404:CDS 35.0%
TCAAGGAATGCAAATCTTGT+TGG - chr4.1:13679963-13679982 MS.gene53404:CDS 35.0%
TGACTAAAATAGGGAGTCTA+TGG - chr4.1:13679918-13679937 MS.gene53404:CDS 35.0%
TGGATTGTGAAAAACTCATG+GGG - chr4.1:13680026-13680045 MS.gene53404:CDS 35.0%
TGTTTGAACTCTTCCAATTG+TGG - chr4.1:13678498-13678517 MS.gene53404:CDS 35.0%
TTACACCAGCATAGAATTGA+AGG + chr4.1:13679608-13679627 None:intergenic 35.0%
TTCAAACACTTCTTCATCAG+AGG + chr4.1:13678486-13678505 None:intergenic 35.0%
TTGATTGCACATACACCATA+AGG + chr4.1:13680110-13680129 None:intergenic 35.0%
TTTGGCTGGGTTATTGAAAA+TGG - chr4.1:13679287-13679306 MS.gene53404:CDS 35.0%
! AAATCTTGTTGGTCTAATCG+TGG - chr4.1:13679974-13679993 MS.gene53404:CDS 35.0%
! AATCTTGTTGGTCTAATCGT+GGG - chr4.1:13679975-13679994 MS.gene53404:CDS 35.0%
! CATTCTAACATCTTAGCTTG+TGG - chr4.1:13679434-13679453 MS.gene53404:intron 35.0%
! CCATAATTTCTTGTGCTGAT+AGG + chr4.1:13679217-13679236 None:intergenic 35.0%
! TAGGTTTTACTAAACTCCTC+AGG + chr4.1:13678683-13678702 None:intergenic 35.0%
! TCTAATCGTGGGTTATGATT+CGG - chr4.1:13679986-13680005 MS.gene53404:CDS 35.0%
!! GTTATTGAAGATTGGCCTTA+TGG - chr4.1:13680092-13680111 MS.gene53404:CDS 35.0%
!! GTTATTTTCTAGAGAGCCAT+TGG - chr4.1:13679129-13679148 MS.gene53404:intron 35.0%
!! TGTTAGTATTGACAACCTTC+TGG - chr4.1:13679506-13679525 MS.gene53404:intron 35.0%
!!! TATTGACAACCTTCTGGTTT+TGG - chr4.1:13679512-13679531 MS.gene53404:intron 35.0%
AAAACTTGTCCAACTTGCCA+TGG + chr4.1:13678463-13678482 None:intergenic 40.0%
AACTCATGGGGAAAAGATTG+GGG - chr4.1:13680038-13680057 MS.gene53404:CDS 40.0%
ACAACCTCTTAGTGTGAGTT+TGG - chr4.1:13679572-13679591 MS.gene53404:intron 40.0%
ACTCTTCCAATTGTGGAAGA+AGG - chr4.1:13678505-13678524 MS.gene53404:CDS 40.0%
CTAAAATAGGGAGTCTATGG+TGG - chr4.1:13679921-13679940 MS.gene53404:CDS 40.0%
GCTGGGTTATTGAAAATGGT+GGG - chr4.1:13679291-13679310 MS.gene53404:CDS 40.0%
GGAATCTGCATCTCTATCTT+TGG + chr4.1:13678710-13678729 None:intergenic 40.0%
TCTGTAGATTGGAGGGAAAA+AGG - chr4.1:13678788-13678807 MS.gene53404:intron 40.0%
TGTATGTGCAATCAATGCTG+CGG - chr4.1:13680115-13680134 MS.gene53404:CDS 40.0%
!! AATTTCTAGCCATGGCAAGT+TGG - chr4.1:13678451-13678470 MS.gene53404:CDS 40.0%
!! GAGTACTCAATTTCTAGCCA+TGG - chr4.1:13678443-13678462 MS.gene53404:CDS 40.0%
AAGGAGCTGTGACTGAAGTT+AGG - chr4.1:13678807-13678826 MS.gene53404:intron 45.0%
ACCTTCCCACTTCTGTAGAT+TGG - chr4.1:13678777-13678796 MS.gene53404:intron 45.0%
AGACTATGCCAATTCAGAGG+AGG - chr4.1:13678538-13678557 MS.gene53404:CDS 45.0%
AGATGCAGATTCCCTCCAAT+CGG - chr4.1:13678717-13678736 MS.gene53404:intron 45.0%
AGGAGCTGTGACTGAAGTTA+GGG - chr4.1:13678808-13678827 MS.gene53404:intron 45.0%
AGTGTGAGTTTGGATGCAAC+TGG - chr4.1:13679582-13679601 MS.gene53404:intron 45.0%
ATTGGGCATTCTCAGTCACT+GGG - chr4.1:13679147-13679166 MS.gene53404:intron 45.0%
CAATTGTGGAAGAAGGAGCA+CGG - chr4.1:13678512-13678531 MS.gene53404:CDS 45.0%
CGGTGAATGGTGAAGACTAT+TGG - chr4.1:13680006-13680025 MS.gene53404:CDS 45.0%
CTGATCCATCCAAAACCAGA+AGG + chr4.1:13679524-13679543 None:intergenic 45.0%
GCATCCAAACTCACACTAAG+AGG + chr4.1:13679579-13679598 None:intergenic 45.0%
GGAAAAGATTGGGGAGAGAA+AGG - chr4.1:13680047-13680066 MS.gene53404:CDS 45.0%
GGCTGGGTTATTGAAAATGG+TGG - chr4.1:13679290-13679309 MS.gene53404:CDS 45.0%
TCCAATCTACAGAAGTGGGA+AGG + chr4.1:13678781-13678800 None:intergenic 45.0%
TCCCACTTCTGTAGATTGGA+GGG - chr4.1:13678781-13678800 MS.gene53404:intron 45.0%
TCCCTCCAATCTACAGAAGT+GGG + chr4.1:13678785-13678804 None:intergenic 45.0%
TTCCCACTTCTGTAGATTGG+AGG - chr4.1:13678780-13678799 MS.gene53404:intron 45.0%
TTCCCTCCAATCTACAGAAG+TGG + chr4.1:13678786-13678805 None:intergenic 45.0%
! AATTGTGGAAGAAGGAGCAC+GGG - chr4.1:13678513-13678532 MS.gene53404:CDS 45.0%
! GGCATTTTCCTCCTCTGAAT+TGG + chr4.1:13678549-13678568 None:intergenic 45.0%
! GTTTGCTGATATGAGTCCTG+AGG - chr4.1:13678664-13678683 MS.gene53404:intron 45.0%
!! ACTGGCCTTCAATTCTATGC+TGG - chr4.1:13679600-13679619 MS.gene53404:intron 45.0%
!! GTGGGTTATGATTCGGTGAA+TGG - chr4.1:13679993-13680012 MS.gene53404:CDS 45.0%
!!! GACAACCTTCTGGTTTTGGA+TGG - chr4.1:13679516-13679535 MS.gene53404:intron 45.0%
AAGCTTCCCATTGTCCCGAT+TGG + chr4.1:13678735-13678754 None:intergenic 50.0%
ATTCCCTCCAATCGGGACAA+TGG - chr4.1:13678725-13678744 MS.gene53404:intron 50.0%
CAGCACAACCCTTACTAGCA+GGG + chr4.1:13679247-13679266 None:intergenic 50.0%
CAGTGACTGAGAATGCCCAA+TGG + chr4.1:13679148-13679167 None:intergenic 50.0%
CATTGGGCATTCTCAGTCAC+TGG - chr4.1:13679146-13679165 MS.gene53404:intron 50.0%
CGTGCTCCTTCTTCCACAAT+TGG + chr4.1:13678514-13678533 None:intergenic 50.0%
GACTGTGACCCTGCTAGTAA+GGG - chr4.1:13679236-13679255 MS.gene53404:CDS 50.0%
GATGCAGATTCCCTCCAATC+GGG - chr4.1:13678718-13678737 MS.gene53404:intron 50.0%
GCGAGACTATGCCAATTCAG+AGG - chr4.1:13678535-13678554 MS.gene53404:CDS 50.0%
GTGACTGAAGTTAGGGACCA+AGG - chr4.1:13678815-13678834 MS.gene53404:intron 50.0%
TCAGTCACTGGGGCAATTGA+AGG - chr4.1:13679158-13679177 MS.gene53404:intron 50.0%
TGTGCAATCAATGCTGCGGT+TGG - chr4.1:13680119-13680138 MS.gene53404:CDS 50.0%
TTCCCATTGTCCCGATTGGA+GGG + chr4.1:13678731-13678750 None:intergenic 50.0%
TTCCCTCCAATCGGGACAAT+GGG - chr4.1:13678726-13678745 MS.gene53404:intron 50.0%
TTGGGCATTCTCAGTCACTG+GGG - chr4.1:13679148-13679167 MS.gene53404:intron 50.0%
CCAGCACAACCCTTACTAGC+AGG + chr4.1:13679248-13679267 None:intergenic 55.0%
CCTGCTAGTAAGGGTTGTGC+TGG - chr4.1:13679245-13679264 MS.gene53404:CDS 55.0%
CTTCCCATTGTCCCGATTGG+AGG + chr4.1:13678732-13678751 None:intergenic 55.0%
GCTAGTAAGGGTTGTGCTGG+AGG - chr4.1:13679248-13679267 MS.gene53404:CDS 55.0%
GGACTGTGACCCTGCTAGTA+AGG - chr4.1:13679235-13679254 MS.gene53404:CDS 55.0%


Chromosome Type Strat End Strand Name
chr4.1 gene 13678359 13680178 13678359 ID=MS.gene53404
chr4.1 mRNA 13678359 13680178 13678359 ID=MS.gene53404.t1;Parent=MS.gene53404
chr4.1 exon 13679692 13680178 13679692 ID=MS.gene53404.t1.exon1;Parent=MS.gene53404.t1
chr4.1 CDS 13679692 13680178 13679692 ID=cds.MS.gene53404.t1;Parent=MS.gene53404.t1
chr4.1 exon 13679173 13679396 13679173 ID=MS.gene53404.t1.exon2;Parent=MS.gene53404.t1
chr4.1 CDS 13679173 13679396 13679173 ID=cds.MS.gene53404.t1;Parent=MS.gene53404.t1
chr4.1 exon 13678914 13679048 13678914 ID=MS.gene53404.t1.exon3;Parent=MS.gene53404.t1
chr4.1 CDS 13678914 13679048 13678914 ID=cds.MS.gene53404.t1;Parent=MS.gene53404.t1
chr4.1 exon 13678359 13678607 13678359 ID=MS.gene53404.t1.exon4;Parent=MS.gene53404.t1
chr4.1 CDS 13678359 13678607 13678359 ID=cds.MS.gene53404.t1;Parent=MS.gene53404.t1
Gene Sequence

>MS.gene53404

ATGATGATATCCCACATCACAAAGTTCATTCTGTCTTTTTTAATCCTCATTTCCATTACATGTCTTTCATTTGCCTTGTCTAGTGAGTACTCAATTTCTAGCCATGGCAAGTTGGACAAGTTTTCCTCTGATGAAGAAGTGTTTGAACTCTTCCAATTGTGGAAGAAGGAGCACGGGCGAGACTATGCCAATTCAGAGGAGGAAAATGCCAAGAGATTTGAGATATTTAAAACCAATTTTAAGTATATCAATGAGATGAATGCAAAGAGAAAATCGCAAACACAACATCGTTTGAGTCTCAACAAGTTTGCTGATATGAGTCCTGAGGAGTTTAGTAAAACCTACTTACCAAAGATAGAGATGCAGATTCCCTCCAATCGGGACAATGGGAAGCTTAGAGATGATGATGATTGTGAAAACCTTCCCACTTCTGTAGATTGGAGGGAAAAAGGAGCTGTGACTGAAGTTAGGGACCAAGGAAATTGTCGTAAGATTTTATATTAATTATACCATTCTTTATTAGTAATTTTCATTTTTGCATTTCATTCCTATATATGTTTATGAATTTAATAATTTTATACCTTTAGAAAGTATATATAGGAATATAGAGATTTGAAGTTATTTTAGATGTTATTATGATGAAAAAAGTTAACTACAAAATATACAAGTGAATTTTTAAGGTACTTTAAAAAGTTAAAATGAAAACGTGTTTATTTTTGCAAGATTAGCTAATAATCCATATATTTTCTTGGACTTATTCAAGTAAATTTGTTATTTTCTAGAGAGCCATTGGGCATTCTCAGTCACTGGGGCAATTGAAGGACTAAACAAAATAGTCACGGGAAATCTTATCAACCTATCAGCACAAGAAATTATGGACTGTGACCCTGCTAGTAAGGGTTGTGCTGGAGGTTTTTACTTTAATGCATTTGGCTGGGTTATTGAAAATGGTGGGATTGATACAGAAGCTAACTATCCTTATTTAGCTAAAAATGGTACTTGCAAGGTATGATTTGCAATTTTTCATTTTCTCCTTAACTATTTGTCTTTTCTAATTAATAAGAAACTTTAGTCACATTCTAACATCTTAGCTTGTGGAACCATCAAATTATATGTTTTTTTTCTTGAAGGAAAATGCAAACAAGGTTGTTAGTATTGACAACCTTCTGGTTTTGGATGGATCAGAAGAAGCACTTTTGTGTCGTTCTAGTAAACAACCTCTTAGTGTGAGTTTGGATGCAACTGGCCTTCAATTCTATGCTGGTGTAAGTATTAGTATTTATTGATATATTAATTGACAAGACTAGTGATTTTGTACAATTTTTTTCTTTTTTTATCAGATTCAACCATAGTTAAACCAATTGACTTTCAATAGTCAAATTTTTAAAACATTAACTTGGTATGCACTTAAGAAATTTTTTTAGGCTAAAGTTTAGTGCTAATGCGAGGTGTCGATATATCTTTCAAAAGGTTGAATCTGGTGTCATCTATCTAATGTGAGACTAGTTATTTCAATAGAAAGTATATATATATATATATATTTAATGAAATTGTTGTTTTGACTAAAATAGGGAGTCTATGGTGGTGAGAACTGCAAAAAAGATTCAAGGAATGCAAATCTTGTTGGTCTAATCGTGGGTTATGATTCGGTGAATGGTGAAGACTATTGGATTGTGAAAAACTCATGGGGAAAAGATTGGGGAGAGAAAGGTTACTTGTTTATCAAAAGGAATGTTATTGAAGATTGGCCTTATGGTGTATGTGCAATCAATGCTGCGGTTGGTTACCCAATCAAAACAGCTTTATCTTCAGCCATTTAG
Protein sequence

>MS.gene53404.t1

MMISHITKFILSFLILISITCLSFALSSEYSISSHGKLDKFSSDEEVFELFQLWKKEHGRDYANSEEENAKRFEIFKTNFKYINEMNAKRKSQTQHRLSLNKFADMSPEEFSKTYLPKIEMQIPSNRDNGKLRDDDDCENLPTSVDWREKGAVTEVRDQGNCQSHWAFSVTGAIEGLNKIVTGNLINLSAQEIMDCDPASKGCAGGFYFNAFGWVIENGGIDTEANYPYLAKNGTCKENANKVVSIDNLLVLDGSEEALLCRSSKQPLSVSLDATGLQFYAGGVYGGENCKKDSRNANLVGLIVGYDSVNGEDYWIVKNSWGKDWGEKGYLFIKRNVIEDWPYGVCAINAAVGYPIKTALSSAI