AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene050901


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene050901.t1 MTR_7g029510 99.029 103 1 0 1 103 1 103 9.33e-68 198
MS.gene050901.t1 MTR_7g099610 99.029 103 1 0 1 103 1 103 9.33e-68 198
MS.gene050901.t1 MTR_5g063620 99.029 103 1 0 1 103 1 103 9.33e-68 198
MS.gene050901.t1 MTR_4g061827 99.029 103 1 0 1 103 1 103 9.33e-68 198
MS.gene050901.t1 MTR_4g061920 99.029 103 1 0 1 103 1 103 9.33e-68 198
MS.gene050901.t1 MTR_3g462400 99.029 103 1 0 1 103 1 103 9.33e-68 198
MS.gene050901.t1 MTR_3g054380 99.029 103 1 0 1 103 1 103 9.33e-68 198
MS.gene050901.t1 MTR_3g462730 99.029 103 1 0 1 103 1 103 9.33e-68 198
MS.gene050901.t1 MTR_3g462510 99.029 103 1 0 1 103 1 103 9.33e-68 198
MS.gene050901.t1 MTR_3g070920 99.029 103 1 0 1 103 1 103 9.33e-68 198
MS.gene050901.t1 MTR_3g061440 99.029 103 1 0 1 103 1 103 9.33e-68 198
MS.gene050901.t1 MTR_3g462410 99.029 103 1 0 1 103 1 103 9.33e-68 198
MS.gene050901.t1 MTR_2g096100 99.029 103 1 0 1 103 1 103 9.33e-68 198
MS.gene050901.t1 MTR_8g038460 99.029 103 1 0 1 103 1 103 9.33e-68 198
MS.gene050901.t1 MTR_4g128150 100.000 101 0 0 1 101 1 101 5.99e-66 203
MS.gene050901.t1 MTR_7g029520 84.000 50 8 0 27 76 1 50 2.85e-21 86.7
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene050901.t1 AT5G59970 99.029 103 1 0 1 103 1 103 9.77e-68 198
MS.gene050901.t1 AT5G59690 99.029 103 1 0 1 103 1 103 9.77e-68 198
MS.gene050901.t1 AT5G59970 99.029 103 1 0 1 103 1 103 9.77e-68 198
MS.gene050901.t1 AT3G46320 99.029 103 1 0 1 103 1 103 9.77e-68 198
MS.gene050901.t1 AT3G45930 99.029 103 1 0 1 103 1 103 9.77e-68 198
MS.gene050901.t1 AT3G53730 99.029 103 1 0 1 103 1 103 9.77e-68 198
MS.gene050901.t1 AT2G28740 99.029 103 1 0 1 103 1 103 9.77e-68 198
MS.gene050901.t1 AT1G07660 99.029 103 1 0 1 103 1 103 9.77e-68 198
MS.gene050901.t1 AT1G07820 99.029 103 1 0 1 103 1 103 9.77e-68 198
MS.gene050901.t1 AT1G07820 99.029 103 1 0 1 103 1 103 9.77e-68 198
MS.gene050901.t1 AT1G07660 82.524 103 1 1 1 103 1 86 1.44e-50 154

Find 32 sgRNAs with CRISPR-Local

Find 29 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
AGGCAGGGAAGGACTTTATA+TGG 0.314150 7.1:+3278305 MS.gene050901:CDS
GGAAAAGGAGGAAAGGGATT+AGG 0.403262 7.1:+3278041 MS.gene050901:CDS
AAAATGTCAGGCAGAGGAAA+AGG 0.476544 7.1:+3278026 None:intergenic
GTGCTTCGTGACAACATTCA+AGG 0.477240 7.1:+3278092 MS.gene050901:CDS
GGCAGAGGAAAAGGAGGAAA+GGG 0.498699 7.1:+3278035 MS.gene050901:CDS
TCACTGCAAGCGAAAATGTC+AGG 0.502586 7.1:+3278014 None:intergenic
CTGCGATTCGTCGTCTTGCA+AGG 0.502831 7.1:+3278126 MS.gene050901:CDS
TGTTTATGCTCTGAAGAGGC+AGG 0.507468 7.1:+3278289 MS.gene050901:CDS
AGGAGGAAAGGGATTAGGAA+AGG 0.515958 7.1:+3278046 MS.gene050901:CDS
GGAGGAAAGGGATTAGGAAA+GGG 0.527356 7.1:+3278047 MS.gene050901:CDS
GGAAAGGGATTAGGAAAGGG+AGG 0.538569 7.1:+3278050 MS.gene050901:CDS
AAGACGACGAATCGCAGGCT+TGG 0.548379 7.1:-3278120 None:intergenic
CTTGCAAGACGACGAATCGC+AGG 0.552192 7.1:-3278125 None:intergenic
AGGCAGAGGAAAAGGAGGAA+AGG 0.557567 7.1:+3278034 MS.gene050901:CDS
ATGCTCTGAAGAGGCAGGGA+AGG 0.564763 7.1:+3278294 MS.gene050901:CDS
AGGACTTTATATGGTTTCAG+TGG 0.568394 7.1:+3278314 MS.gene050901:CDS
GAGGAAGACTGTTACCGCCA+TGG 0.588179 7.1:+3278262 MS.gene050901:CDS
CAAGCGAAAATGTCAGGCAG+AGG 0.590069 7.1:+3278020 None:intergenic
CATTCTCGAGGAAGATCTTG+AGG 0.597682 7.1:-3278202 None:intergenic
TTACATACACTGAACACGCT+AGG 0.600176 7.1:+3278240 MS.gene050901:CDS
GTTTATGCTCTGAAGAGGCA+GGG 0.605263 7.1:+3278290 MS.gene050901:CDS
ATTCGTCGTCTTGCAAGGCG+TGG 0.611450 7.1:+3278131 MS.gene050901:CDS
GGCGGTGTTAAGAGAATCAG+TGG 0.619776 7.1:+3278152 MS.gene050901:CDS
CAGAGCATAAACAACATCCA+TGG 0.631933 7.1:-3278279 None:intergenic
CATACACTGAACACGCTAGG+AGG 0.635713 7.1:+3278243 MS.gene050901:CDS
CGTCGTCTTGCAAGGCGTGG+CGG 0.636706 7.1:+3278134 MS.gene050901:CDS
ATGTCAGGCAGAGGAAAAGG+AGG 0.661131 7.1:+3278029 MS.gene050901:CDS
ATGTTGTTTATGCTCTGAAG+AGG 0.672733 7.1:+3278285 MS.gene050901:CDS
TAGGAAAGGGAGGAGCAAAG+AGG 0.691969 7.1:+3278060 MS.gene050901:CDS
CTCATATATGAAGAAACTCG+CGG 0.708309 7.1:+3278176 MS.gene050901:CDS
CATCACGAATCACATTCTCG+AGG 0.731876 7.1:-3278214 None:intergenic
AGCATAAACAACATCCATGG+CGG 0.735882 7.1:-3278276 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
AGGACTTTATATGGTTTCAG+TGG + chr7.1:3278314-3278333 MS.gene050901:CDS 35.0%
CTCATATATGAAGAAACTCG+CGG + chr7.1:3278176-3278195 MS.gene050901:CDS 35.0%
!! ATGTTGTTTATGCTCTGAAG+AGG + chr7.1:3278285-3278304 MS.gene050901:CDS 35.0%
AGCATAAACAACATCCATGG+CGG - chr7.1:3278279-3278298 None:intergenic 40.0%
CAGAGCATAAACAACATCCA+TGG - chr7.1:3278282-3278301 None:intergenic 40.0%
TTACATACACTGAACACGCT+AGG + chr7.1:3278240-3278259 MS.gene050901:CDS 40.0%
AGGAGGAAAGGGATTAGGAA+AGG + chr7.1:3278046-3278065 MS.gene050901:CDS 45.0%
AGGCAGGGAAGGACTTTATA+TGG + chr7.1:3278305-3278324 MS.gene050901:CDS 45.0%
CATCACGAATCACATTCTCG+AGG - chr7.1:3278217-3278236 None:intergenic 45.0%
CATTCTCGAGGAAGATCTTG+AGG - chr7.1:3278205-3278224 None:intergenic 45.0%
GGAAAAGGAGGAAAGGGATT+AGG + chr7.1:3278041-3278060 MS.gene050901:CDS 45.0%
GGAGGAAAGGGATTAGGAAA+GGG + chr7.1:3278047-3278066 MS.gene050901:CDS 45.0%
GTGCTTCGTGACAACATTCA+AGG + chr7.1:3278092-3278111 MS.gene050901:CDS 45.0%
!! GTTTATGCTCTGAAGAGGCA+GGG + chr7.1:3278290-3278309 MS.gene050901:CDS 45.0%
!! TGTTTATGCTCTGAAGAGGC+AGG + chr7.1:3278289-3278308 MS.gene050901:CDS 45.0%
AGGCAGAGGAAAAGGAGGAA+AGG + chr7.1:3278034-3278053 MS.gene050901:CDS 50.0%
ATGTCAGGCAGAGGAAAAGG+AGG + chr7.1:3278029-3278048 MS.gene050901:CDS 50.0%
CATACACTGAACACGCTAGG+AGG + chr7.1:3278243-3278262 MS.gene050901:CDS 50.0%
GGAAAGGGATTAGGAAAGGG+AGG + chr7.1:3278050-3278069 MS.gene050901:CDS 50.0%
GGCAGAGGAAAAGGAGGAAA+GGG + chr7.1:3278035-3278054 MS.gene050901:CDS 50.0%
TAGGAAAGGGAGGAGCAAAG+AGG + chr7.1:3278060-3278079 MS.gene050901:CDS 50.0%
! GGCGGTGTTAAGAGAATCAG+TGG + chr7.1:3278152-3278171 MS.gene050901:CDS 50.0%
AAGACGACGAATCGCAGGCT+TGG - chr7.1:3278123-3278142 None:intergenic 55.0%
ATTCGTCGTCTTGCAAGGCG+TGG + chr7.1:3278131-3278150 MS.gene050901:CDS 55.0%
CTGCGATTCGTCGTCTTGCA+AGG + chr7.1:3278126-3278145 MS.gene050901:CDS 55.0%
CTTGCAAGACGACGAATCGC+AGG - chr7.1:3278128-3278147 None:intergenic 55.0%
GAGGAAGACTGTTACCGCCA+TGG + chr7.1:3278262-3278281 MS.gene050901:CDS 55.0%
!! ATGCTCTGAAGAGGCAGGGA+AGG + chr7.1:3278294-3278313 MS.gene050901:CDS 55.0%
CGTCGTCTTGCAAGGCGTGG+CGG + chr7.1:3278134-3278153 MS.gene050901:CDS 65.0%


Chromosome Type Strat End Strand Name
chr7.1 gene 3278029 3278340 3278029 ID=MS.gene050901
chr7.1 mRNA 3278029 3278340 3278029 ID=MS.gene050901.t1;Parent=MS.gene050901
chr7.1 exon 3278029 3278340 3278029 ID=MS.gene050901.t1.exon1;Parent=MS.gene050901.t1
chr7.1 CDS 3278029 3278340 3278029 ID=cds.MS.gene050901.t1;Parent=MS.gene050901.t1
Gene Sequence

>MS.gene050901

ATGTCAGGCAGAGGAAAAGGAGGAAAGGGATTAGGAAAGGGAGGAGCAAAGAGGCACAGAAAAGTGCTTCGTGACAACATTCAAGGAATAACCAAGCCTGCGATTCGTCGTCTTGCAAGGCGTGGCGGTGTTAAGAGAATCAGTGGTCTCATATATGAAGAAACTCGCGGTGTCCTCAAGATCTTCCTCGAGAATGTGATTCGTGATGCTGTTACATACACTGAACACGCTAGGAGGAAGACTGTTACCGCCATGGATGTTGTTTATGCTCTGAAGAGGCAGGGAAGGACTTTATATGGTTTCAGTGGTTGA

Protein sequence

>MS.gene050901.t1

MSGRGKGGKGLGKGGAKRHRKVLRDNIQGITKPAIRRLARRGGVKRISGLIYEETRGVLKIFLENVIRDAVTYTEHARRKTVTAMDVVYALKRQGRTLYGFSG