AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene53438

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene53438.t1 MTR_4g065077 95.330 364 17 0 1 364 1 364 0.0 731
MS.gene53438.t1 MTR_8g086470 90.934 364 17 1 1 364 17 364 0.0 688
MS.gene53438.t1 MTR_2g015920 46.575 365 180 8 2 357 1 359 7.59e-108 321
MS.gene53438.t1 MTR_2g016180 50.435 345 160 8 18 357 18 356 7.53e-107 322
MS.gene53438.t1 MTR_2g016270 50.145 345 146 8 18 357 18 341 8.89e-105 316
MS.gene53438.t1 MTR_2g015930 46.499 357 176 9 9 357 10 359 3.75e-102 306
MS.gene53438.t1 MTR_3g116080 41.292 356 181 14 14 357 9 348 6.54e-78 243
MS.gene53438.t1 MTR_4g094918 39.678 373 185 12 6 363 1 348 9.36e-77 241
MS.gene53438.t1 MTR_3g102220 41.758 364 185 13 10 357 10 362 2.05e-76 243
MS.gene53438.t1 MTR_3g112430 42.500 320 166 9 49 359 34 344 8.14e-76 241
MS.gene53438.t1 MTR_3g075390 40.730 356 186 13 15 359 6 347 3.05e-74 234
MS.gene53438.t1 MTR_1g018840 39.894 376 192 14 2 357 1 362 4.35e-74 237
MS.gene53438.t1 MTR_1g018840 39.894 376 192 14 2 357 1 362 7.18e-74 237
MS.gene53438.t1 MTR_4g079440 39.779 362 193 14 2 356 1 344 9.17e-74 233
MS.gene53438.t1 MTR_1g107550 42.308 338 171 13 32 357 31 356 1.36e-73 236
MS.gene53438.t1 MTR_2g090890 41.401 314 162 11 51 357 39 337 3.99e-70 223
MS.gene53438.t1 MTR_4g080700 40.063 317 164 11 49 355 38 338 9.07e-69 219
MS.gene53438.t1 MTR_4g080730 40.379 317 163 11 49 355 38 338 3.40e-68 218
MS.gene53438.t1 MTR_4g047610 38.994 318 179 8 45 355 32 341 3.91e-68 218
MS.gene53438.t1 MTR_7g078600 40.575 313 166 9 49 356 40 337 2.63e-67 216
MS.gene53438.t1 MTR_4g104370 39.665 358 187 15 14 359 6 346 3.03e-67 216
MS.gene53438.t1 MTR_5g022560 38.318 321 179 9 44 355 32 342 4.26e-66 213
MS.gene53438.t1 MTR_4g079470 36.957 322 187 8 41 355 31 343 4.86e-64 207
MS.gene53438.t1 MTR_4g079770 37.304 319 184 8 44 355 34 343 5.13e-64 207
MS.gene53438.t1 MTR_4g080360 36.512 367 182 14 9 356 7 341 9.08e-64 207
MS.gene53438.t1 MTR_4g107930 37.695 321 179 10 45 355 34 343 1.63e-63 206
MS.gene53438.t1 MTR_2g075830 35.457 361 186 11 11 355 10 339 1.26e-62 204
MS.gene53438.t1 MTR_7g079230 39.423 312 170 11 49 355 37 334 1.78e-62 203
MS.gene53438.t1 MTR_4g079800 36.760 321 183 9 44 355 34 343 8.14e-62 202
MS.gene53438.t1 MTR_2g083930 37.963 324 178 12 46 356 30 343 1.55e-60 198
MS.gene53438.t1 MTR_1g094135 32.888 374 211 11 6 352 2 362 3.67e-55 186
MS.gene53438.t1 MTR_1g023210 35.423 319 176 12 51 352 51 356 5.55e-55 184
MS.gene53438.t1 MTR_4g071060 33.155 374 204 13 3 352 1 352 9.37e-55 184
MS.gene53438.t1 MTR_2g015850 39.726 292 161 9 71 356 81 363 1.27e-53 181
MS.gene53438.t1 MTR_2g067900 36.533 323 169 10 52 354 17 323 5.53e-53 178
MS.gene53438.t1 MTR_2g067560 35.826 321 168 12 51 348 16 321 1.78e-50 181
MS.gene53438.t1 MTR_4g125300 31.215 362 227 9 3 356 1 348 7.46e-49 168
MS.gene53438.t1 MTR_2g064475 33.918 342 176 13 54 355 19 350 5.20e-48 166
MS.gene53438.t1 MTR_4g072790 31.232 349 190 11 14 352 8 316 3.74e-41 147
MS.gene53438.t1 MTR_1g023810 38.009 221 126 6 139 354 47 261 1.64e-40 144
MS.gene53438.t1 MTR_2g066980 32.915 319 159 14 54 355 37 317 2.22e-40 145
MS.gene53438.t1 MTR_4g073660 31.161 353 189 12 14 352 7 319 3.53e-40 145
MS.gene53438.t1 MTR_4g072340 33.010 309 164 10 51 352 48 320 3.84e-40 145
MS.gene53438.t1 MTR_7g078630 33.696 276 139 8 40 301 695 940 2.86e-35 137
MS.gene53438.t1 MTR_2g016380 46.218 119 59 3 241 357 21 136 1.43e-26 106
MS.gene53438.t1 MTR_2g015840 26.496 351 173 11 8 355 2 270 2.09e-26 106
MS.gene53438.t1 MTR_1g025450 39.655 174 79 7 139 306 53 206 3.53e-26 110
MS.gene53438.t1 MTR_7g078310 36.879 141 77 4 93 229 112 244 5.97e-22 94.4
MS.gene53438.t1 MTR_7g078580 44.954 109 50 6 250 356 1 101 7.56e-19 82.0
MS.gene53438.t1 MTR_3g029560 30.841 214 114 9 152 356 49 237 4.36e-18 82.8
MS.gene53438.t1 MTR_8g087510 30.709 127 84 3 182 306 23 147 1.34e-14 71.6
MS.gene53438.t1 MTR_7g079130 42.708 96 46 5 264 356 8 97 5.57e-14 67.8
MS.gene53438.t1 MTR_7g111050 26.230 244 136 9 141 352 100 331 6.83e-12 66.2
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene53438.t1 AT5G43060 41.944 360 180 12 13 357 9 354 1.92e-82 259
MS.gene53438.t1 AT4G23520 39.516 372 197 13 2 363 3 356 1.39e-80 250
MS.gene53438.t1 AT4G35350 41.598 363 186 14 6 358 8 354 1.50e-79 248
MS.gene53438.t1 AT1G20850 44.543 339 166 13 29 358 30 355 1.11e-78 246
MS.gene53438.t1 AT1G09850 43.252 326 157 10 45 358 26 335 1.48e-78 248
MS.gene53438.t1 AT1G47128 40.331 362 192 12 9 357 3 353 7.25e-77 244
MS.gene53438.t1 AT4G11310 39.452 365 191 13 13 362 9 358 5.61e-75 236
MS.gene53438.t1 AT5G50260 41.341 358 183 14 13 359 4 345 1.48e-74 235
MS.gene53438.t1 AT4G11320 37.197 371 210 11 4 362 6 365 1.43e-72 230
MS.gene53438.t1 AT4G11320 37.197 371 210 11 4 362 6 365 1.43e-72 230
MS.gene53438.t1 AT4G36880 40.571 350 184 12 25 357 20 362 9.29e-72 228
MS.gene53438.t1 AT3G48340 38.315 368 189 12 11 362 4 349 5.62e-71 226
MS.gene53438.t1 AT3G19400 40.663 332 176 13 43 364 36 356 8.50e-70 223
MS.gene53438.t1 AT3G19390 38.211 369 200 16 2 361 1 350 7.43e-69 223
MS.gene53438.t1 AT3G48350 37.883 359 194 15 11 357 3 344 6.07e-67 216
MS.gene53438.t1 AT1G06260 37.394 353 197 10 12 357 8 343 7.78e-67 214
MS.gene53438.t1 AT1G29090 39.335 361 182 14 13 356 14 354 1.89e-66 214
MS.gene53438.t1 AT2G34080 36.517 356 205 12 10 356 1 344 3.09e-66 213
MS.gene53438.t1 AT5G45890 35.598 368 200 14 1 355 1 344 6.13e-65 210
MS.gene53438.t1 AT3G43960 40.244 328 178 12 46 364 36 354 8.72e-65 210
MS.gene53438.t1 AT1G29080 36.134 357 205 12 9 356 3 345 1.97e-64 208
MS.gene53438.t1 AT3G49340 35.942 345 198 11 22 356 9 340 9.75e-64 206
MS.gene53438.t1 AT2G27420 37.363 364 194 15 10 356 1 347 6.99e-61 199
MS.gene53438.t1 AT4G35350 40.070 287 152 11 6 282 8 284 4.98e-57 187
MS.gene53438.t1 AT1G29110 33.894 357 191 13 15 356 7 333 5.06e-56 186
MS.gene53438.t1 AT3G54940 35.758 330 181 9 51 362 51 367 1.26e-55 186
MS.gene53438.t1 AT4G39090 34.234 333 183 11 51 363 51 367 6.87e-54 182
MS.gene53438.t1 AT5G60360 34.936 312 183 8 51 356 59 356 1.48e-52 178
MS.gene53438.t1 AT2G21430 35.974 303 164 11 51 333 48 340 1.42e-51 176
MS.gene53438.t1 AT5G60360 36.585 287 168 7 51 331 59 337 2.27e-51 175
MS.gene53438.t1 AT5G60360 36.585 287 168 7 51 331 59 337 3.34e-51 174
MS.gene53438.t1 AT4G16190 31.551 374 205 14 13 352 6 362 1.25e-49 171
MS.gene53438.t1 AT3G48350 35.254 295 169 11 11 294 3 286 9.53e-49 166
MS.gene53438.t1 AT3G45310 33.333 312 188 9 51 356 59 356 3.70e-48 166
MS.gene53438.t1 AT3G45310 35.192 287 172 8 51 331 59 337 1.53e-47 165
MS.gene53438.t1 AT3G19400 40.909 242 130 8 46 279 39 275 1.93e-47 162
MS.gene53438.t1 AT3G54940 36.139 202 116 3 51 243 51 248 8.41e-36 132
MS.gene53438.t1 AT4G01610 28.870 239 126 11 141 342 103 334 2.25e-12 67.8

Find 87 sgRNAs with CRISPR-Local

Find 118 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CTCATTGATATACTTAAAAT+TGG 0.205591 4.3:+14076991 None:intergenic
CCTATCAGCACAAGAAATTA+TGG 0.278118 4.3:-14076370 MS.gene53438:CDS
TATTGACAACCTTCTAGTTT+TGG 0.290143 4.3:-14076071 MS.gene53438:CDS
CGGTGAATGGTGAAGACTAT+TGG 0.304070 4.3:-14075548 MS.gene53438:CDS
TTTACTTTAATGCGTTTGGC+TGG 0.308198 4.3:-14076311 MS.gene53438:CDS
GTTATTGAAGATTGGCCTTA+TGG 0.314718 4.3:-14075462 MS.gene53438:CDS
CCATAATTTCTTGTGCTGAT+AGG 0.338269 4.3:+14076370 None:intergenic
CGTGCTCCTTCTTCCACAAT+TGG 0.339247 4.3:+14077071 None:intergenic
ACAACCTCTTAGTGTGAGTT+TGG 0.347332 4.3:-14076011 MS.gene53438:CDS
CATCTCTATCTTTGGTAAGT+AGG 0.351412 4.3:+14076883 None:intergenic
TTTGGCTGGGTTATTGAAAA+TGG 0.356971 4.3:-14076297 MS.gene53438:CDS
ACCTTCCCACTTCTGTAGAT+TGG 0.361626 4.3:-14076805 MS.gene53438:CDS
AAGGAGCTGTGACTGAAGTT+AGG 0.364375 4.3:-14076775 MS.gene53438:CDS
CATTGGGCATTCTCAGTCAC+TGG 0.368149 4.3:-14076438 MS.gene53438:CDS
AAAGGAATGTTATTGAAGAT+TGG 0.377330 4.3:-14075470 MS.gene53438:CDS
TTTAAATATCTCAAATCTCT+TGG 0.377666 4.3:+14077015 None:intergenic
TGACTAAAATAGGGAGTCTA+TGG 0.386085 4.3:-14075636 MS.gene53438:intron
TCTGTAGATTGGAGGGAAAA+AGG 0.392044 4.3:-14076794 MS.gene53438:CDS
AAACTCATGGGGAAAAGATT+GGG 0.397399 4.3:-14075517 MS.gene53438:CDS
GGAATCTGCATCTCTATCTT+TGG 0.403742 4.3:+14076875 None:intergenic
AAGGTTACTTGTTTATCAAA+AGG 0.408095 4.3:-14075488 MS.gene53438:CDS
GGACTGTGACCCTGCTAGTA+AGG 0.414218 4.3:-14076349 MS.gene53438:CDS
CTGATCCATCCAAAACTAGA+AGG 0.428334 4.3:+14076062 None:intergenic
GGCAAATGAAAGACATGTAA+TGG 0.429471 4.3:+14077171 None:intergenic
TCAGTCACTGGGGCAATTGA+AGG 0.431508 4.3:-14076426 MS.gene53438:CDS
TCAAGGAATGCAAATCTTGT+TGG 0.438289 4.3:-14075591 MS.gene53438:CDS
AAAACTCATGGGGAAAAGAT+TGG 0.442756 4.3:-14075518 MS.gene53438:CDS
AAATCTTACGACAATTTCCT+TGG 0.443422 4.3:+14076750 None:intergenic
TTCCCTCCAATCGGGACAAT+GGG 0.454262 4.3:-14076856 MS.gene53438:CDS
TCTAATCGTGGGTTATGATT+CGG 0.463790 4.3:-14075568 MS.gene53438:CDS
GGCTGGGTTATTGAAAATGG+TGG 0.476570 4.3:-14076294 MS.gene53438:CDS
AGGAGCTGTGACTGAAGTTA+GGG 0.480944 4.3:-14076774 MS.gene53438:CDS
TTACACCAGCATAGAATTGA+AGG 0.485435 4.3:+14075978 None:intergenic
AAGCTTCCCATTGTCCCGAT+TGG 0.485909 4.3:+14076850 None:intergenic
AGTGTGAGTTTGGATGCAAC+TGG 0.487456 4.3:-14076001 MS.gene53438:CDS
AGATGCAGATTCCCTCCAAT+CGG 0.487597 4.3:-14076865 MS.gene53438:CDS
CCTGCTAGTAAGGGTTGTGC+TGG 0.489644 4.3:-14076339 MS.gene53438:CDS
CTTGAAGGAAAATGCAAACA+AGG 0.490268 4.3:-14076101 MS.gene53438:intron
GCATCCAAACTCACACTAAG+AGG 0.491176 4.3:+14076007 None:intergenic
CCAGCACAACCCTTACTAGC+AGG 0.492421 4.3:+14076339 None:intergenic
TTACTTTAATGCGTTTGGCT+GGG 0.494755 4.3:-14076310 MS.gene53438:CDS
CTTCCCATTGTCCCGATTGG+AGG 0.510526 4.3:+14076853 None:intergenic
ATTCCCTCCAATCGGGACAA+TGG 0.514126 4.3:-14076857 MS.gene53438:CDS
ACTCTTCCAATTGTGGAAGA+AGG 0.517582 4.3:-14077077 MS.gene53438:CDS
GGAAAAGATTGGGGAGAGAA+AGG 0.518533 4.3:-14075507 MS.gene53438:CDS
GCTAGTAAGGGTTGTGCTGG+AGG 0.520226 4.3:-14076336 MS.gene53438:CDS
ACTGGCCTTCAATTCTATGC+TGG 0.521431 4.3:-14075983 MS.gene53438:CDS
TTCCCACTTCTGTAGATTGG+AGG 0.523180 4.3:-14076802 MS.gene53438:CDS
TTCCCTCCAATCTACAGAAG+TGG 0.526210 4.3:+14076799 None:intergenic
TCCCTCCAATCTACAGAAGT+GGG 0.527833 4.3:+14076800 None:intergenic
AAAGACATGTAATGGAAATG+AGG 0.531576 4.3:+14077179 None:intergenic
TGTTTGAACTCTTCCAATTG+TGG 0.531957 4.3:-14077084 MS.gene53438:CDS
CTTTATCTTCAGCCATGTAG+TGG 0.532318 4.3:-14075395 None:intergenic
TTGATTGCACATACACCATA+AGG 0.533070 4.3:+14075447 None:intergenic
GTGGGTTATGATTCGGTGAA+TGG 0.534697 4.3:-14075561 MS.gene53438:CDS
TGCTCTAACCAACCACTACA+TGG 0.537247 4.3:+14075383 None:intergenic
GATGCAGATTCCCTCCAATC+GGG 0.537566 4.3:-14076864 MS.gene53438:CDS
TTCCCATTGTCCCGATTGGA+GGG 0.544474 4.3:+14076854 None:intergenic
GCTGGGTTATTGAAAATGGT+GGG 0.551387 4.3:-14076293 MS.gene53438:CDS
AATCTTGTTGGTCTAATCGT+GGG 0.553988 4.3:-14075579 MS.gene53438:CDS
TCCCACTTCTGTAGATTGGA+GGG 0.556871 4.3:-14076801 MS.gene53438:CDS
TTCAAACACTTCTTCATCAG+AGG 0.560590 4.3:+14077099 None:intergenic
AATTTCTAGCCATGGCAAGT+TGG 0.565364 4.3:-14077131 MS.gene53438:CDS
GACTGTGACCCTGCTAGTAA+GGG 0.569928 4.3:-14076348 MS.gene53438:CDS
ATTGGGCATTCTCAGTCACT+GGG 0.573858 4.3:-14076437 MS.gene53438:CDS
AAATCTTGTTGGTCTAATCG+TGG 0.581439 4.3:-14075580 MS.gene53438:CDS
CAGAATGAACTTTGTGATGT+GGG 0.582146 4.3:+14077213 None:intergenic
GGACTAAACAAAATAGTCAC+GGG 0.593846 4.3:-14076405 MS.gene53438:CDS
AAAACTTGTCCAACTTGCCA+TGG 0.594424 4.3:+14077122 None:intergenic
GAGTACTCAATTTCTAGCCA+TGG 0.600592 4.3:-14077139 MS.gene53438:CDS
AATTGTGGAAGAAGGAGCAC+GGG 0.602772 4.3:-14077069 MS.gene53438:CDS
CTAAAATAGGGAGTCTATGG+TGG 0.604269 4.3:-14075633 MS.gene53438:intron
CAATTGTGGAAGAAGGAGCA+CGG 0.616767 4.3:-14077070 MS.gene53438:CDS
ACAGAATGAACTTTGTGATG+TGG 0.621850 4.3:+14077212 None:intergenic
AGGACTAAACAAAATAGTCA+CGG 0.622654 4.3:-14076406 MS.gene53438:CDS
GTGACTGAAGTTAGGGACCA+AGG 0.628455 4.3:-14076767 MS.gene53438:CDS
AACTCATGGGGAAAAGATTG+GGG 0.629466 4.3:-14075516 MS.gene53438:CDS
TCCAATCTACAGAAGTGGGA+AGG 0.633136 4.3:+14076804 None:intergenic
CAGCACAACCCTTACTAGCA+GGG 0.633326 4.3:+14076340 None:intergenic
TGTGCAATCAATGCTGCGGT+TGG 0.646120 4.3:-14075435 MS.gene53438:CDS
CAGTGACTGAGAATGCCCAA+TGG 0.662774 4.3:+14076439 None:intergenic
AATTGAGTACTCACTAGACA+AGG 0.669581 4.3:+14077150 None:intergenic
AGACTATGCCAATTCAGAGG+AGG 0.671634 4.3:-14077044 MS.gene53438:CDS
GCGAGACTATGCCAATTCAG+AGG 0.673652 4.3:-14077047 MS.gene53438:CDS
GTTTGCTGATATGAGTCCTG+AGG 0.681956 4.3:-14076918 MS.gene53438:CDS
TTGGGCATTCTCAGTCACTG+GGG 0.757924 4.3:-14076436 MS.gene53438:CDS
TGTATGTGCAATCAATGCTG+CGG 0.774207 4.3:-14075439 MS.gene53438:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! AAAAAAAACATATAATTTGA+TGG + chr4.3:14076499-14076518 None:intergenic 10.0%
!! TTAAATTCATAAACATATAT+AGG + chr4.3:14075948-14075967 None:intergenic 10.0%
!!! AATATATAAGTGAATTTTTA+AGG - chr4.3:14076054-14076073 MS.gene53438:CDS 10.0%
!! TGAAAATTACTAATAAAGAA+TGG + chr4.3:14075910-14075929 None:intergenic 15.0%
!! TTAGAAAAGACAAATAATTA+AGG + chr4.3:14076431-14076450 None:intergenic 15.0%
!!! CAAGTTAATGTTTTAAAAAT+TGG + chr4.3:14076758-14076777 None:intergenic 15.0%
!!! CAATTTTTAAAACATTAACT+TGG - chr4.3:14076756-14076775 MS.gene53438:intron 15.0%
!!! TAATAATCCATATATTTTCT+TGG - chr4.3:14076125-14076144 MS.gene53438:intron 15.0%
!!! TATATGTTTTTTTTTCTTGA+AGG - chr4.3:14076505-14076524 MS.gene53438:intron 15.0%
!! AATAAGTCCAAGAAAATATA+TGG + chr4.3:14076135-14076154 None:intergenic 20.0%
!! CTCATTGATATACTTAAAAT+TGG + chr4.3:14075633-14075652 None:intergenic 20.0%
!! TACCTTTACAAAGTATATAT+AGG - chr4.3:14075976-14075995 MS.gene53438:intron 20.0%
!! TTCCTATATATACTTTGTAA+AGG + chr4.3:14075981-14076000 None:intergenic 20.0%
!! TTTAAATATCTCAAATCTCT+TGG + chr4.3:14075609-14075628 None:intergenic 20.0%
!!! ATTGTTGTTTTGACTAAAAT+AGG - chr4.3:14076975-14076994 MS.gene53438:CDS 20.0%
!!! TATCCTTATTTAGCTAAAAA+TGG - chr4.3:14076369-14076388 MS.gene53438:CDS 20.0%
!!! TTGTTGTTTTGACTAAAATA+GGG - chr4.3:14076976-14076995 MS.gene53438:CDS 20.0%
! AAAGGAATGTTATTGAAGAT+TGG - chr4.3:14077151-14077170 MS.gene53438:CDS 25.0%
! AAGGTTACTTGTTTATCAAA+AGG - chr4.3:14077133-14077152 MS.gene53438:CDS 25.0%
! GTGTTCATATATGTTTCAAA+AGG - chr4.3:14076827-14076846 MS.gene53438:CDS 25.0%
! TTAGATATATAGATCACATC+AGG + chr4.3:14076858-14076877 None:intergenic 25.0%
!!! AAGATAAAGCTGTTTTGATT+GGG + chr4.3:14077215-14077234 None:intergenic 25.0%
!!! GTACCATTTTTAGCTAAATA+AGG + chr4.3:14076375-14076394 None:intergenic 25.0%
AAATCTTACGACAATTTCCT+TGG + chr4.3:14075874-14075893 None:intergenic 30.0%
AGAACTGCAAAAAAGATTCA+AGG - chr4.3:14077013-14077032 MS.gene53438:CDS 30.0%
AGGACTAAACAAAATAGTCA+CGG - chr4.3:14076215-14076234 MS.gene53438:intron 30.0%
ATTGGATTGTGAAAAACTCA+TGG - chr4.3:14077091-14077110 MS.gene53438:CDS 30.0%
TAAACTTTAGTGCTAATGTG+AGG - chr4.3:14076805-14076824 MS.gene53438:CDS 30.0%
TTGGATTGTGAAAAACTCAT+GGG - chr4.3:14077092-14077111 MS.gene53438:CDS 30.0%
! AAAGACATGTAATGGAAATG+AGG + chr4.3:14075445-14075464 None:intergenic 30.0%
! GGTTTTTACTTTAATGCGTT+TGG - chr4.3:14076306-14076325 MS.gene53438:CDS 30.0%
!! TTATTTTCTAGAGAGCCATT+GGG - chr4.3:14076167-14076186 MS.gene53438:intron 30.0%
!!! GAAGATAAAGCTGTTTTGAT+TGG + chr4.3:14077216-14077235 None:intergenic 30.0%
!!! TATTGACAACCTTCTAGTTT+TGG - chr4.3:14076550-14076569 MS.gene53438:intron 30.0%
AAAACTCATGGGGAAAAGAT+TGG - chr4.3:14077103-14077122 MS.gene53438:CDS 35.0%
AAACTCATGGGGAAAAGATT+GGG - chr4.3:14077104-14077123 MS.gene53438:CDS 35.0%
AATTGAGTACTCACTAGACA+AGG + chr4.3:14075474-14075493 None:intergenic 35.0%
ACAGAATGAACTTTGTGATG+TGG + chr4.3:14075412-14075431 None:intergenic 35.0%
AGCTAAAAATGGTACTTGCA+AGG - chr4.3:14076380-14076399 MS.gene53438:CDS 35.0%
CAGAATGAACTTTGTGATGT+GGG + chr4.3:14075411-14075430 None:intergenic 35.0%
CATCTCTATCTTTGGTAAGT+AGG + chr4.3:14075741-14075760 None:intergenic 35.0%
CATTCTAACATCTTTGCTTG+TGG - chr4.3:14076471-14076490 MS.gene53438:intron 35.0%
CCTATCAGCACAAGAAATTA+TGG - chr4.3:14076251-14076270 MS.gene53438:CDS 35.0%
CTTGAAGGAAAATGCAAACA+AGG - chr4.3:14076520-14076539 MS.gene53438:intron 35.0%
GGACTAAACAAAATAGTCAC+GGG - chr4.3:14076216-14076235 MS.gene53438:intron 35.0%
GGCAAATGAAAGACATGTAA+TGG + chr4.3:14075453-14075472 None:intergenic 35.0%
TCAAGGAATGCAAATCTTGT+TGG - chr4.3:14077030-14077049 MS.gene53438:CDS 35.0%
TGACTAAAATAGGGAGTCTA+TGG - chr4.3:14076985-14077004 MS.gene53438:CDS 35.0%
TGGATTGTGAAAAACTCATG+GGG - chr4.3:14077093-14077112 MS.gene53438:CDS 35.0%
TGTTTGAACTCTTCCAATTG+TGG - chr4.3:14075537-14075556 MS.gene53438:CDS 35.0%
TTACACCAGCATAGAATTGA+AGG + chr4.3:14076646-14076665 None:intergenic 35.0%
TTACTTTAATGCGTTTGGCT+GGG - chr4.3:14076311-14076330 MS.gene53438:CDS 35.0%
TTCAAACACTTCTTCATCAG+AGG + chr4.3:14075525-14075544 None:intergenic 35.0%
TTGATTGCACATACACCATA+AGG + chr4.3:14077177-14077196 None:intergenic 35.0%
TTTACTTTAATGCGTTTGGC+TGG - chr4.3:14076310-14076329 MS.gene53438:CDS 35.0%
TTTGGCTGGGTTATTGAAAA+TGG - chr4.3:14076324-14076343 MS.gene53438:CDS 35.0%
! AAATCTTGTTGGTCTAATCG+TGG - chr4.3:14077041-14077060 MS.gene53438:CDS 35.0%
! AATCTTGTTGGTCTAATCGT+GGG - chr4.3:14077042-14077061 MS.gene53438:CDS 35.0%
! CCATAATTTCTTGTGCTGAT+AGG + chr4.3:14076254-14076273 None:intergenic 35.0%
! TAGGTTTTACTAAACTCCTC+AGG + chr4.3:14075722-14075741 None:intergenic 35.0%
! TCTAATCGTGGGTTATGATT+CGG - chr4.3:14077053-14077072 MS.gene53438:CDS 35.0%
!! GTTATTGAAGATTGGCCTTA+TGG - chr4.3:14077159-14077178 MS.gene53438:CDS 35.0%
!! GTTATTTTCTAGAGAGCCAT+TGG - chr4.3:14076166-14076185 MS.gene53438:intron 35.0%
AAAACTTGTCCAACTTGCCA+TGG + chr4.3:14075502-14075521 None:intergenic 40.0%
AACTCATGGGGAAAAGATTG+GGG - chr4.3:14077105-14077124 MS.gene53438:CDS 40.0%
ACAACCTCTTAGTGTGAGTT+TGG - chr4.3:14076610-14076629 MS.gene53438:intron 40.0%
ACTCTTCCAATTGTGGAAGA+AGG - chr4.3:14075544-14075563 MS.gene53438:CDS 40.0%
CTAAAATAGGGAGTCTATGG+TGG - chr4.3:14076988-14077007 MS.gene53438:CDS 40.0%
CTGATCCATCCAAAACTAGA+AGG + chr4.3:14076562-14076581 None:intergenic 40.0%
GCTGGGTTATTGAAAATGGT+GGG - chr4.3:14076328-14076347 MS.gene53438:CDS 40.0%
GGAATCTGCATCTCTATCTT+TGG + chr4.3:14075749-14075768 None:intergenic 40.0%
TCTGTAGATTGGAGGGAAAA+AGG - chr4.3:14075827-14075846 MS.gene53438:intron 40.0%
TGTATGTGCAATCAATGCTG+CGG - chr4.3:14077182-14077201 MS.gene53438:CDS 40.0%
!! AATTTCTAGCCATGGCAAGT+TGG - chr4.3:14075490-14075509 MS.gene53438:CDS 40.0%
!! GAGTACTCAATTTCTAGCCA+TGG - chr4.3:14075482-14075501 MS.gene53438:CDS 40.0%
!!! GACAACCTTCTAGTTTTGGA+TGG - chr4.3:14076554-14076573 MS.gene53438:intron 40.0%
AAGGAGCTGTGACTGAAGTT+AGG - chr4.3:14075846-14075865 MS.gene53438:intron 45.0%
ACCTTCCCACTTCTGTAGAT+TGG - chr4.3:14075816-14075835 MS.gene53438:intron 45.0%
AGACTATGCCAATTCAGAGG+AGG - chr4.3:14075577-14075596 MS.gene53438:CDS 45.0%
AGATGCAGATTCCCTCCAAT+CGG - chr4.3:14075756-14075775 MS.gene53438:intron 45.0%
AGGAGCTGTGACTGAAGTTA+GGG - chr4.3:14075847-14075866 MS.gene53438:intron 45.0%
AGTGTGAGTTTGGATGCAAC+TGG - chr4.3:14076620-14076639 MS.gene53438:intron 45.0%
ATTGGGCATTCTCAGTCACT+GGG - chr4.3:14076184-14076203 MS.gene53438:intron 45.0%
CAATTGTGGAAGAAGGAGCA+CGG - chr4.3:14075551-14075570 MS.gene53438:CDS 45.0%
CGGTGAATGGTGAAGACTAT+TGG - chr4.3:14077073-14077092 MS.gene53438:CDS 45.0%
GCATCCAAACTCACACTAAG+AGG + chr4.3:14076617-14076636 None:intergenic 45.0%
GGAAAAGATTGGGGAGAGAA+AGG - chr4.3:14077114-14077133 MS.gene53438:CDS 45.0%
GGCTGGGTTATTGAAAATGG+TGG - chr4.3:14076327-14076346 MS.gene53438:CDS 45.0%
TCCAATCTACAGAAGTGGGA+AGG + chr4.3:14075820-14075839 None:intergenic 45.0%
TCCCACTTCTGTAGATTGGA+GGG - chr4.3:14075820-14075839 MS.gene53438:intron 45.0%
TCCCTCCAATCTACAGAAGT+GGG + chr4.3:14075824-14075843 None:intergenic 45.0%
TTCCCACTTCTGTAGATTGG+AGG - chr4.3:14075819-14075838 MS.gene53438:intron 45.0%
TTCCCTCCAATCTACAGAAG+TGG + chr4.3:14075825-14075844 None:intergenic 45.0%
! AATTGTGGAAGAAGGAGCAC+GGG - chr4.3:14075552-14075571 MS.gene53438:CDS 45.0%
! GGCATTTTCCTCCTCTGAAT+TGG + chr4.3:14075588-14075607 None:intergenic 45.0%
! GTTTGCTGATATGAGTCCTG+AGG - chr4.3:14075703-14075722 MS.gene53438:intron 45.0%
!! ACTGGCCTTCAATTCTATGC+TGG - chr4.3:14076638-14076657 MS.gene53438:intron 45.0%
!! GTGGGTTATGATTCGGTGAA+TGG - chr4.3:14077060-14077079 MS.gene53438:CDS 45.0%
AAGCTTCCCATTGTCCCGAT+TGG + chr4.3:14075774-14075793 None:intergenic 50.0%
ATTCCCTCCAATCGGGACAA+TGG - chr4.3:14075764-14075783 MS.gene53438:intron 50.0%
CAGCACAACCCTTACTAGCA+GGG + chr4.3:14076284-14076303 None:intergenic 50.0%
CAGTGACTGAGAATGCCCAA+TGG + chr4.3:14076185-14076204 None:intergenic 50.0%
CATTGGGCATTCTCAGTCAC+TGG - chr4.3:14076183-14076202 MS.gene53438:intron 50.0%
CGTGCTCCTTCTTCCACAAT+TGG + chr4.3:14075553-14075572 None:intergenic 50.0%
GACTGTGACCCTGCTAGTAA+GGG - chr4.3:14076273-14076292 MS.gene53438:CDS 50.0%
GATGCAGATTCCCTCCAATC+GGG - chr4.3:14075757-14075776 MS.gene53438:intron 50.0%
GCGAGACTATGCCAATTCAG+AGG - chr4.3:14075574-14075593 MS.gene53438:CDS 50.0%
GTGACTGAAGTTAGGGACCA+AGG - chr4.3:14075854-14075873 MS.gene53438:intron 50.0%
TCAGTCACTGGGGCAATTGA+AGG - chr4.3:14076195-14076214 MS.gene53438:intron 50.0%
TGTGCAATCAATGCTGCGGT+TGG - chr4.3:14077186-14077205 MS.gene53438:CDS 50.0%
TTCCCATTGTCCCGATTGGA+GGG + chr4.3:14075770-14075789 None:intergenic 50.0%
TTCCCTCCAATCGGGACAAT+GGG - chr4.3:14075765-14075784 MS.gene53438:intron 50.0%
TTGGGCATTCTCAGTCACTG+GGG - chr4.3:14076185-14076204 MS.gene53438:intron 50.0%
CCAGCACAACCCTTACTAGC+AGG + chr4.3:14076285-14076304 None:intergenic 55.0%
CCTGCTAGTAAGGGTTGTGC+TGG - chr4.3:14076282-14076301 MS.gene53438:CDS 55.0%
CTTCCCATTGTCCCGATTGG+AGG + chr4.3:14075771-14075790 None:intergenic 55.0%
GCTAGTAAGGGTTGTGCTGG+AGG - chr4.3:14076285-14076304 MS.gene53438:CDS 55.0%
GGACTGTGACCCTGCTAGTA+AGG - chr4.3:14076272-14076291 MS.gene53438:CDS 55.0%


Chromosome Type Strat End Strand Name
chr4.3 gene 14075398 14077245 14075398 ID=MS.gene53438
chr4.3 mRNA 14075398 14077245 14075398 ID=MS.gene53438.t1;Parent=MS.gene53438
chr4.3 exon 14076759 14077245 14076759 ID=MS.gene53438.t1.exon1;Parent=MS.gene53438.t1
chr4.3 CDS 14076759 14077245 14076759 ID=cds.MS.gene53438.t1;Parent=MS.gene53438.t1
chr4.3 exon 14076242 14076465 14076242 ID=MS.gene53438.t1.exon2;Parent=MS.gene53438.t1
chr4.3 CDS 14076242 14076465 14076242 ID=cds.MS.gene53438.t1;Parent=MS.gene53438.t1
chr4.3 exon 14075982 14076116 14075982 ID=MS.gene53438.t1.exon3;Parent=MS.gene53438.t1
chr4.3 CDS 14075982 14076116 14075982 ID=cds.MS.gene53438.t1;Parent=MS.gene53438.t1
chr4.3 exon 14075398 14075646 14075398 ID=MS.gene53438.t1.exon4;Parent=MS.gene53438.t1
chr4.3 CDS 14075398 14075646 14075398 ID=cds.MS.gene53438.t1;Parent=MS.gene53438.t1
Gene Sequence

>MS.gene53438

ATGATGATATCCCACATCACAAAGTTCATTCTGTCTTTTTTAATCCTCATTTCCATTACATGTCTTTCATTTGCCTTGTCTAGTGAGTACTCAATTTCTAGCCATGGCAAGTTGGACAAGTTTTCCTCTGATGAAGAAGTGTTTGAACTCTTCCAATTGTGGAAGAAGGAGCACGGGCGAGACTATGCCAATTCAGAGGAGGAAAATGCCAAGAGATTTGAGATATTTAAAACCAATTTTAAGTATATCAATGAGATGAATGCAAAGAGAAAATCGCAAACACAACATCGTTTGAGTCTCAACAAGTTTGCTGATATGAGTCCTGAGGAGTTTAGTAAAACCTACTTACCAAAGATAGAGATGCAGATTCCCTCCAATCGGGACAATGGGAAGCTTAGAGATGATGATGATTGTGAAAACCTTCCCACTTCTGTAGATTGGAGGGAAAAAGGAGCTGTGACTGAAGTTAGGGACCAAGGAAATTGTCGTAAGATTTTATATTAATTATACCATTCTTTATTAGTAATTTTCATTTTTGCATTTCATTCCTATATATGTTTATGAATTTAATTATTTTATACCTTTACAAAGTATATATAGGAATAGAGATTTGAAGTTATTTTAGATGTTATTATGATGAAAAAAGTTAACTACAAAATATATAAGTGAATTTTTAAGGTACTTTAAAAAGTTAAAATGAAAACGTGTTTATTTTTGCAAGATTAGCTAATAATCCATATATTTTCTTGGACTTATTCAAGTAAATTTGTTATTTTCTAGAGAGCCATTGGGCATTCTCAGTCACTGGGGCAATTGAAGGACTAAACAAAATAGTCACGGGAAATCTTATCAACCTATCAGCACAAGAAATTATGGACTGTGACCCTGCTAGTAAGGGTTGTGCTGGAGGTTTTTACTTTAATGCGTTTGGCTGGGTTATTGAAAATGGTGGGATTGATACAGAAGCTAACTATCCTTATTTAGCTAAAAATGGTACTTGCAAGGTATGATTTGCAATTTTTCATTTTCTCCTTAATTATTTGTCTTTTCTAATTAATAAGAAACTTTACTCACATTCTAACATCTTTGCTTGTGGAACCATCAAATTATATGTTTTTTTTTCTTGAAGGAAAATGCAAACAAGGTTGTTAGTATTGACAACCTTCTAGTTTTGGATGGATCAGAAGAAGCACTCTTGTGTCGTACTAGTAAACAACCTCTTAGTGTGAGTTTGGATGCAACTGGCCTTCAATTCTATGCTGGTGTAAGTATTAGTATTTATTGATATATTAATTGACAAGACTAGTCATTTTGTACAATTTTTTCTTATTTTATTTATCAATTGAATCTCTATAGTCCAATTTTTAAAACATTAACTTGGTATGTACTTAAGACATTTTTTTAGACTAAACTTTAGTGCTAATGTGAGGTGTTCATATATGTTTCAAAAGGTTGAACCTGATGTGATCTATATATCTAATGTGAGACTAGTTATTTCAATAGAAAGTATATATATATATATATATATATATATATATATATATATATATATATATATATATATATATATTAATGAAATTGTTGTTTTGACTAAAATAGGGAGTCTATGGTGGTGAGAACTGCAAAAAAGATTCAAGGAATGCAAATCTTGTTGGTCTAATCGTGGGTTATGATTCGGTGAATGGTGAAGACTATTGGATTGTGAAAAACTCATGGGGAAAAGATTGGGGAGAGAAAGGTTACTTGTTTATCAAAAGGAATGTTATTGAAGATTGGCCTTATGGTGTATGTGCAATCAATGCTGCGGTTGGTTACCCAATCAAAACAGCTTTATCTTCAGCCATGTAG
Protein sequence

>MS.gene53438.t1

MMISHITKFILSFLILISITCLSFALSSEYSISSHGKLDKFSSDEEVFELFQLWKKEHGRDYANSEEENAKRFEIFKTNFKYINEMNAKRKSQTQHRLSLNKFADMSPEEFSKTYLPKIEMQIPSNRDNGKLRDDDDCENLPTSVDWREKGAVTEVRDQGNCQSHWAFSVTGAIEGLNKIVTGNLINLSAQEIMDCDPASKGCAGGFYFNAFGWVIENGGIDTEANYPYLAKNGTCKENANKVVSIDNLLVLDGSEEALLCRTSKQPLSVSLDATGLQFYAGGVYGGENCKKDSRNANLVGLIVGYDSVNGEDYWIVKNSWGKDWGEKGYLFIKRNVIEDWPYGVCAINAAVGYPIKTALSSAM