Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene53557.t1 | XP_003590175.2 | 95.9 | 145 | 6 | 0 | 1 | 145 | 152 | 296 | 4.10E-68 | 267.3 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene53557.t1 | G7I6P2 | 95.9 | 145 | 6 | 0 | 1 | 145 | 152 | 296 | 3.0e-68 | 267.3 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene051949 | MS.gene53557 | 0.823521 | 1.32E-53 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene53557.t1 | MTR_1g045520 | 95.862 | 145 | 6 | 0 | 1 | 145 | 152 | 296 | 2.88e-87 | 256 |
| MS.gene53557.t1 | MTR_4g088590 | 62.651 | 83 | 7 | 1 | 1 | 83 | 74 | 132 | 1.49e-16 | 72.0 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene53557.t1 | AT3G04310 | 36.486 | 148 | 87 | 4 | 1 | 145 | 125 | 268 | 4.17e-19 | 81.3 |
| MS.gene53557.t1 | AT3G04310 | 36.486 | 148 | 87 | 4 | 1 | 145 | 125 | 268 | 4.17e-19 | 81.3 |
Find 40 sgRNAs with CRISPR-Local
Find 51 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| CACTAGCATTTGAGAAATTA+TGG | 0.154846 | 1.2:+28452017 | MS.gene53557:CDS |
| TTCATCCTATACTTGCTCTT+TGG | 0.267500 | 1.2:-28452058 | None:intergenic |
| GCTTGAGATCTCCTTGTTGT+TGG | 0.332877 | 1.2:-28452259 | None:intergenic |
| AGCCGAGATACACCAAGTTT+TGG | 0.371713 | 1.2:+28452208 | MS.gene53557:CDS |
| ACTAGCATTTGAGAAATTAT+GGG | 0.409975 | 1.2:+28452018 | MS.gene53557:CDS |
| CTCGTGTATCGTTTACATTT+CGG | 0.423090 | 1.2:-28452090 | None:intergenic |
| CTTGCTCTTTGGTTTGCTCT+CGG | 0.443826 | 1.2:-28452047 | None:intergenic |
| AGGGTCACAAAGGATGAATA+TGG | 0.454789 | 1.2:+28452288 | MS.gene53557:CDS |
| AAAACGAATACGAGGAAGGC+AGG | 0.470687 | 1.2:+28452139 | MS.gene53557:CDS |
| CACCAAAACTTGGTGTATCT+CGG | 0.471519 | 1.2:-28452210 | None:intergenic |
| TGGAAGGTGGTAGGTTGAGT+AGG | 0.473865 | 1.2:+28452308 | MS.gene53557:CDS |
| GCAAACCAAAGAGCAAGTAT+AGG | 0.487735 | 1.2:+28452053 | MS.gene53557:CDS |
| TCATCCCATCCACCAAAACT+TGG | 0.508215 | 1.2:-28452220 | None:intergenic |
| GGAGATCTCAAGCAAGGAAA+GGG | 0.523252 | 1.2:+28452269 | MS.gene53557:CDS |
| AGAGCAAGTATAGGATGAAG+AGG | 0.539420 | 1.2:+28452062 | MS.gene53557:CDS |
| AACAACGGTTCGGTTGATAG+TGG | 0.539590 | 1.2:+28452184 | MS.gene53557:CDS |
| AATAAGCAATCGAACCAAGA+GGG | 0.559725 | 1.2:-28452353 | None:intergenic |
| GGAAGGCAGGGATGCAATCG+TGG | 0.559821 | 1.2:+28452152 | MS.gene53557:CDS |
| AGGAGATCTCAAGCAAGGAA+AGG | 0.568063 | 1.2:+28452268 | MS.gene53557:CDS |
| TACACGAGTTGAGGAAGAGC+CGG | 0.568386 | 1.2:+28452105 | MS.gene53557:CDS |
| GGTAGTTGAGAACAACGGTT+CGG | 0.582840 | 1.2:+28452174 | MS.gene53557:CDS |
| TGTAAACGATACACGAGTTG+AGG | 0.583956 | 1.2:+28452096 | MS.gene53557:CDS |
| GGATGAATATGGAAGGTGGT+AGG | 0.584534 | 1.2:+28452299 | MS.gene53557:CDS |
| GGGATGACTTGGAGAGAGCA+AGG | 0.588570 | 1.2:+28452236 | MS.gene53557:CDS |
| CATTTGAGAAATTATGGGGA+CGG | 0.588715 | 1.2:+28452023 | MS.gene53557:CDS |
| CCAGAAAACGAATACGAGGA+AGG | 0.603804 | 1.2:+28452135 | MS.gene53557:CDS |
| CTAGCATTTGAGAAATTATG+GGG | 0.620286 | 1.2:+28452019 | MS.gene53557:CDS |
| AGAGAGCAAGGCCAACAACA+AGG | 0.623233 | 1.2:+28452248 | MS.gene53557:CDS |
| GAAGGCAGGGATGCAATCGT+GGG | 0.637402 | 1.2:+28452153 | MS.gene53557:CDS |
| CAACAAGGAGATCTCAAGCA+AGG | 0.639362 | 1.2:+28452263 | MS.gene53557:CDS |
| AAACGAATACGAGGAAGGCA+GGG | 0.640599 | 1.2:+28452140 | MS.gene53557:CDS |
| CAAAGGATGAATATGGAAGG+TGG | 0.642176 | 1.2:+28452295 | MS.gene53557:CDS |
| TCACAAAGGATGAATATGGA+AGG | 0.645284 | 1.2:+28452292 | MS.gene53557:CDS |
| ATAAGCAATCGAACCAAGAG+GGG | 0.649272 | 1.2:-28452352 | None:intergenic |
| AAGCAAGGAAAGGGTCACAA+AGG | 0.661727 | 1.2:+28452278 | MS.gene53557:CDS |
| AAAACCAGAAAACGAATACG+AGG | 0.662375 | 1.2:+28452131 | MS.gene53557:CDS |
| GCAAGTGCTGACTGACTGAG+AGG | 0.672424 | 1.2:-28451995 | None:intergenic |
| CAAGTGCTGACTGACTGAGA+GGG | 0.679661 | 1.2:-28451994 | None:intergenic |
| CAATAAGCAATCGAACCAAG+AGG | 0.681035 | 1.2:-28452354 | None:intergenic |
| TCGTGGGTAGTTGAGAACAA+CGG | 0.734928 | 1.2:+28452169 | MS.gene53557:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !!! | ATTGCTATTTTTCCATTTTT+GGG | + | chr1.2:28452373-28452392 | MS.gene53557:CDS | 20.0% |
| !!! | TATTGCTATTTTTCCATTTT+TGG | + | chr1.2:28452372-28452391 | MS.gene53557:CDS | 20.0% |
| ! | ACTAGCATTTGAGAAATTAT+GGG | + | chr1.2:28452018-28452037 | MS.gene53557:CDS | 25.0% |
| CACTAGCATTTGAGAAATTA+TGG | + | chr1.2:28452017-28452036 | MS.gene53557:CDS | 30.0% | |
| CTAGCATTTGAGAAATTATG+GGG | + | chr1.2:28452019-28452038 | MS.gene53557:CDS | 30.0% | |
| AAAACCAGAAAACGAATACG+AGG | + | chr1.2:28452131-28452150 | MS.gene53557:CDS | 35.0% | |
| AATAAGCAATCGAACCAAGA+GGG | - | chr1.2:28452356-28452375 | None:intergenic | 35.0% | |
| CATTTGAGAAATTATGGGGA+CGG | + | chr1.2:28452023-28452042 | MS.gene53557:CDS | 35.0% | |
| CTCGTGTATCGTTTACATTT+CGG | - | chr1.2:28452093-28452112 | None:intergenic | 35.0% | |
| TCACAAAGGATGAATATGGA+AGG | + | chr1.2:28452292-28452311 | MS.gene53557:CDS | 35.0% | |
| TTCATCCTATACTTGCTCTT+TGG | - | chr1.2:28452061-28452080 | None:intergenic | 35.0% | |
| !! | TTTTTCCATTTTTGGGCACA+TGG | + | chr1.2:28452380-28452399 | MS.gene53557:CDS | 35.0% |
| !!! | TTCGTTTTCTGGTTTTCTTC+CGG | - | chr1.2:28452127-28452146 | None:intergenic | 35.0% |
| AGAGCAAGTATAGGATGAAG+AGG | + | chr1.2:28452062-28452081 | MS.gene53557:CDS | 40.0% | |
| AGGGTCACAAAGGATGAATA+TGG | + | chr1.2:28452288-28452307 | MS.gene53557:CDS | 40.0% | |
| ATAAGCAATCGAACCAAGAG+GGG | - | chr1.2:28452355-28452374 | None:intergenic | 40.0% | |
| CAAAGGATGAATATGGAAGG+TGG | + | chr1.2:28452295-28452314 | MS.gene53557:CDS | 40.0% | |
| CAATAAGCAATCGAACCAAG+AGG | - | chr1.2:28452357-28452376 | None:intergenic | 40.0% | |
| GCAAACCAAAGAGCAAGTAT+AGG | + | chr1.2:28452053-28452072 | MS.gene53557:CDS | 40.0% | |
| TGTAAACGATACACGAGTTG+AGG | + | chr1.2:28452096-28452115 | MS.gene53557:CDS | 40.0% | |
| !! | ATACACCAAGTTTTGGTGGA+TGG | + | chr1.2:28452215-28452234 | MS.gene53557:CDS | 40.0% |
| !! | CACCAAAACTTGGTGTATCT+CGG | - | chr1.2:28452213-28452232 | None:intergenic | 40.0% |
| !! | TACACCAAGTTTTGGTGGAT+GGG | + | chr1.2:28452216-28452235 | MS.gene53557:CDS | 40.0% |
| AAAAAGTGATACGCCCCTCT+TGG | + | chr1.2:28452339-28452358 | MS.gene53557:CDS | 45.0% | |
| AAAACGAATACGAGGAAGGC+AGG | + | chr1.2:28452139-28452158 | MS.gene53557:CDS | 45.0% | |
| AAACGAATACGAGGAAGGCA+GGG | + | chr1.2:28452140-28452159 | MS.gene53557:CDS | 45.0% | |
| AAGCAAGGAAAGGGTCACAA+AGG | + | chr1.2:28452278-28452297 | MS.gene53557:CDS | 45.0% | |
| AGGAGATCTCAAGCAAGGAA+AGG | + | chr1.2:28452268-28452287 | MS.gene53557:CDS | 45.0% | |
| CAACAAGGAGATCTCAAGCA+AGG | + | chr1.2:28452263-28452282 | MS.gene53557:CDS | 45.0% | |
| CCAGAAAACGAATACGAGGA+AGG | + | chr1.2:28452135-28452154 | MS.gene53557:CDS | 45.0% | |
| GGAGATCTCAAGCAAGGAAA+GGG | + | chr1.2:28452269-28452288 | MS.gene53557:CDS | 45.0% | |
| GGATGAATATGGAAGGTGGT+AGG | + | chr1.2:28452299-28452318 | MS.gene53557:CDS | 45.0% | |
| GGTAGTTGAGAACAACGGTT+CGG | + | chr1.2:28452174-28452193 | MS.gene53557:CDS | 45.0% | |
| TCATCCCATCCACCAAAACT+TGG | - | chr1.2:28452223-28452242 | None:intergenic | 45.0% | |
| TCGTGGGTAGTTGAGAACAA+CGG | + | chr1.2:28452169-28452188 | MS.gene53557:CDS | 45.0% | |
| TTGGTCCATGTGCCCAAAAA+TGG | - | chr1.2:28452388-28452407 | None:intergenic | 45.0% | |
| ! | AGCCGAGATACACCAAGTTT+TGG | + | chr1.2:28452208-28452227 | MS.gene53557:CDS | 45.0% |
| ! | CCTTCCTCGTATTCGTTTTC+TGG | - | chr1.2:28452138-28452157 | None:intergenic | 45.0% |
| ! | GCTTGAGATCTCCTTGTTGT+TGG | - | chr1.2:28452262-28452281 | None:intergenic | 45.0% |
| !! | AACAACGGTTCGGTTGATAG+TGG | + | chr1.2:28452184-28452203 | MS.gene53557:CDS | 45.0% |
| !! | CGAGATACACCAAGTTTTGG+TGG | + | chr1.2:28452211-28452230 | MS.gene53557:CDS | 45.0% |
| !! | CTTGCTCTTTGGTTTGCTCT+CGG | - | chr1.2:28452050-28452069 | None:intergenic | 45.0% |
| !! | TTTTGGTGGATGGGATGACT+TGG | + | chr1.2:28452225-28452244 | MS.gene53557:CDS | 45.0% |
| AGAGAGCAAGGCCAACAACA+AGG | + | chr1.2:28452248-28452267 | MS.gene53557:CDS | 50.0% | |
| ! | TACACGAGTTGAGGAAGAGC+CGG | + | chr1.2:28452105-28452124 | MS.gene53557:CDS | 50.0% |
| ! | TGGAAGGTGGTAGGTTGAGT+AGG | + | chr1.2:28452308-28452327 | MS.gene53557:CDS | 50.0% |
| !! | CAAGTGCTGACTGACTGAGA+GGG | - | chr1.2:28451997-28452016 | None:intergenic | 50.0% |
| GAAGGCAGGGATGCAATCGT+GGG | + | chr1.2:28452153-28452172 | MS.gene53557:CDS | 55.0% | |
| ! | GGGATGACTTGGAGAGAGCA+AGG | + | chr1.2:28452236-28452255 | MS.gene53557:CDS | 55.0% |
| !! | GCAAGTGCTGACTGACTGAG+AGG | - | chr1.2:28451998-28452017 | None:intergenic | 55.0% |
| GGAAGGCAGGGATGCAATCG+TGG | + | chr1.2:28452152-28452171 | MS.gene53557:CDS | 60.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr1.2 | gene | 28451980 | 28452417 | 28451980 | ID=MS.gene53557 |
| chr1.2 | mRNA | 28451980 | 28452417 | 28451980 | ID=MS.gene53557.t1;Parent=MS.gene53557 |
| chr1.2 | exon | 28451980 | 28452417 | 28451980 | ID=MS.gene53557.t1.exon1;Parent=MS.gene53557.t1 |
| chr1.2 | CDS | 28451980 | 28452417 | 28451980 | ID=cds.MS.gene53557.t1;Parent=MS.gene53557.t1 |
| Gene Sequence |
| Protein sequence |
