AlfalfaGEDB | MS.gene53961

M. sativa cultivar XinJiangDaYe / MS.gene53961

KEGG: K01183
GO: GO:0003674 GO:0003824 GO:0004553 GO:0004568 GO:0005488 GO:0005975 GO:0006022 GO:0006026 GO:0006030 GO:0006032 GO:0006040 GO:0006807 GO:0008061 GO:0008144 GO:0008150 GO:0008152 GO:0009056 GO:0009057 GO:0009987 GO:0016787 GO:0016798 GO:0016998 GO:0017144 GO:0042737 GO:0043170 GO:0044036 GO:0044237 GO:0044238 GO:0044248 GO:0044260 GO:0044265 GO:0046348 GO:0071704 GO:0097367 GO:1901071 GO:1901072 GO:1901135 GO:1901136 GO:1901564 GO:1901565 GO:1901575
PFAM: PF00182.22 PF00187.22

Nr:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene53961.t1	ACL36992.1	99.2	258	2	0	1	258	25	282	1.90E-145	525

Swissprot:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene53961.t1	P27054	76.4	259	56	3	1	258	16	270	3.1e-107	389.4

Trembl:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene53961.t1	B8Y647	99.2	258	2	0	1	258	25	282	1.4e-145	525.0

TFs/TRs:

Gene ID	Type	Classification

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network:

Gene1	Gene2	correlation coefficient	p_value	FDR
MS.gene052878	MS.gene53961	0.998459	1.05E-265	-1.69E-46

PPI:

Gene1	Gene2	Type

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene53961.t1	MTR_2g099470	97.674	258	6	0	1	258	25	282	0.0	527
MS.gene53961.t1	MTR_2g099465	81.140	228	40	2	34	258	27	254	1.08e-140	395
MS.gene53961.t1	MTR_3g118390	38.567	293	126	9	8	258	80	360	2.80e-57	187
MS.gene53961.t1	MTR_8g074350	38.095	294	136	8	8	258	23	313	5.02e-55	180
MS.gene53961.t1	MTR_7g110780	39.241	237	108	6	57	258	36	271	9.41e-54	175
MS.gene53961.t1	MTR_8g074330	37.584	298	137	9	6	258	20	313	1.71e-53	176
MS.gene53961.t1	MTR_8g074335	37.282	287	140	9	7	258	21	302	2.22e-47	159
MS.gene53961.t1	MTR_2g099450	59.167	120	20	2	138	257	84	174	2.52e-37	130
MS.gene53961.t1	MTR_2g099450	80.357	56	11	0	1	56	25	80	2.24e-27	104
MS.gene53961.t1	MTR_2g034680	31.120	241	121	8	62	258	62	301	3.48e-24	99.0
MS.gene53961.t1	MTR_1g105535	29.185	233	119	6	67	258	80	307	6.09e-21	90.5
MS.gene53961.t1	MTR_7g021640	28.182	220	114	7	82	258	86	304	5.94e-19	84.7

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene53961.t1	AT3G54420	71.315	251	67	2	8	258	28	273	4.66e-134	379
MS.gene53961.t1	AT2G43590	56.863	255	100	3	4	258	20	264	5.50e-106	308
MS.gene53961.t1	AT2G43580	52.941	255	111	3	4	258	20	265	1.01e-96	284
MS.gene53961.t1	AT2G43570	52.652	264	107	7	2	258	25	277	4.15e-87	260
MS.gene53961.t1	AT2G43620	46.863	271	123	8	1	258	21	283	3.84e-82	248
MS.gene53961.t1	AT2G43610	47.328	262	119	7	8	258	28	281	9.87e-81	244
MS.gene53961.t1	AT3G47540	46.875	224	96	5	36	258	13	214	2.73e-63	197
MS.gene53961.t1	AT3G47540	46.396	222	94	6	36	255	13	211	7.23e-59	188
MS.gene53961.t1	AT1G56680	40.149	269	141	11	1	258	21	280	4.64e-58	186
MS.gene53961.t1	AT1G56680	40.149	269	141	11	1	258	44	303	1.33e-57	186
MS.gene53961.t1	AT3G12500	39.286	280	132	6	13	258	45	320	1.09e-55	182
MS.gene53961.t1	AT1G02360	39.496	238	108	5	56	258	29	265	2.91e-53	174
MS.gene53961.t1	AT1G02360	39.496	238	108	5	56	258	81	317	8.65e-53	174
MS.gene53961.t1	AT1G02360	39.496	238	108	5	56	258	75	311	9.16e-53	174
MS.gene53961.t1	AT2G43600	36.667	270	148	11	1	258	15	273	1.12e-51	170
MS.gene53961.t1	AT2G43600	36.667	270	148	11	1	258	36	294	2.91e-51	169
MS.gene53961.t1	AT4G01700	38.912	239	110	5	55	258	36	273	3.12e-50	166
MS.gene53961.t1	AT1G05850	26.609	233	125	7	67	258	77	304	9.54e-21	89.7
MS.gene53961.t1	AT1G05850	26.609	233	125	7	67	258	77	304	9.54e-21	89.7
MS.gene53961.t1	AT3G16920	27.126	247	134	9	57	258	68	313	3.10e-19	85.9
MS.gene53961.t1	AT3G16920	27.016	248	135	9	56	258	91	337	5.19e-19	85.5

Find 0 sgRNAs with CRISPR-Local

Find 75 sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region

CRISPR-GE

badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content
!!	CTTAATTCTTATAATCAGTT+TGG	+	31046:81138-81157	MS.gene53961:CDS	20.0%
!!!	AAATTTGGTAACACAATTAT+TGG	+	31046:81334-81353	MS.gene53961:intron	20.0%
!!!	AATTTGGTAACACAATTATT+GGG	+	31046:81335-81354	MS.gene53961:intron	20.0%
!	ATTCTTATAATCAGTTTGGT+AGG	+	31046:81142-81161	MS.gene53961:CDS	25.0%
!	TATTGAAGAAATCTTGTGTA+AGG	-	31046:81047-81066	None:intergenic	25.0%
!!	CTCATATTCTCTTTTTACTA+GGG	+	31046:81381-81400	MS.gene53961:intron	25.0%
!!	TCTCATATTCTCTTTTTACT+AGG	+	31046:81380-81399	MS.gene53961:intron	25.0%
!!	TTTTGAGCACTAACATTTTT+TGG	-	31046:80874-80893	None:intergenic	25.0%
!!!	CGTTAATTTTGTTTAGTTGA+TGG	-	31046:81278-81297	None:intergenic	25.0%
	AATGTTAGTGCTCAAAATTG+TGG	+	31046:80877-80896	MS.gene53961:CDS	30.0%
	ACTCAATATTGTAGTGAGTT+GGG	+	31046:81802-81821	MS.gene53961:CDS	30.0%
	ATGTTAGTGCTCAAAATTGT+GGG	+	31046:80878-80897	MS.gene53961:CDS	30.0%
	TAGTTCCAAGATAGTTGAAT+CGG	-	31046:81556-81575	None:intergenic	30.0%
	TATTGGATGAATAATGTGCA+CGG	+	31046:81676-81695	MS.gene53961:CDS	30.0%
	ATACCATAATGCTGTCTTGA+AGG	-	31046:81659-81678	None:intergenic	35.0%
	CACTCAATATTGTAGTGAGT+TGG	+	31046:81801-81820	MS.gene53961:CDS	35.0%
	CTATCTTGGAACTACAACTA+TGG	+	31046:81562-81581	MS.gene53961:CDS	35.0%
	TAGCAAGTAAGATTATCACC+AGG	-	31046:81835-81854	None:intergenic	35.0%
	TATAATCAGTTTGGTAGGTC+TGG	+	31046:81147-81166	MS.gene53961:CDS	35.0%
	TCAAGACAGCATTATGGTAT+TGG	+	31046:81659-81678	MS.gene53961:CDS	35.0%
	TGCTATACTGAAGAGATTGA+TGG	+	31046:81454-81473	MS.gene53961:CDS	35.0%
	ACCGTAGTAACCTTTGTTAG+GGG	-	31046:81527-81546	None:intergenic	40.0%
	AGCCACATTAGCATCATTGT+TGG	-	31046:81019-81038	None:intergenic	40.0%
	ATTGAACTCTCCTGAAACAG+TGG	+	31046:81615-81634	MS.gene53961:CDS	40.0%
	CATTACCACAATACCCGTAT+TGG	-	31046:80927-80946	None:intergenic	40.0%
	GACCGTAGTAACCTTTGTTA+GGG	-	31046:81528-81547	None:intergenic	40.0%
	GCAACCATTAGGGCTATTAA+TGG	+	31046:81721-81740	MS.gene53961:CDS	40.0%
	GCCACATTAGCATCATTGTT+GGG	-	31046:81018-81037	None:intergenic	40.0%
	GCTCATTTCACACATGAAAC+TGG	+	31046:81210-81229	MS.gene53961:CDS	40.0%
	GTGATGAAAACAACACAGAG+TGG	+	31046:81494-81513	MS.gene53961:CDS	40.0%
	TCATCACAGTAGTCCTTTGA+TGG	-	31046:81481-81500	None:intergenic	40.0%
	TCGACCATTAATAGCCCTAA+TGG	-	31046:81728-81747	None:intergenic	40.0%
	TGTCCTTCAAGACAGCATTA+TGG	+	31046:81653-81672	MS.gene53961:CDS	40.0%
!	AAACAAGGTCCTTGTTATGC+AGG	+	31046:80973-80992	MS.gene53961:CDS	40.0%
!	AGAGATTGATGGTCCATCAA+AGG	+	31046:81465-81484	MS.gene53961:CDS	40.0%
!	CAATACGGGTATTGTGGTAA+TGG	+	31046:80925-80944	MS.gene53961:CDS	40.0%
!	GGTAATGGTGATGCATATTG+TGG	+	31046:80940-80959	MS.gene53961:CDS	40.0%
!!	AATGGTCGACTTGAATGTGA+TGG	+	31046:81739-81758	MS.gene53961:CDS	40.0%
!!!	GGAAGAGACAATGGTTTTGA+TGG	+	31046:81592-81611	MS.gene53961:CDS	40.0%
	AAACCATTGTCTCTTCCAGC+TGG	-	31046:81589-81608	None:intergenic	45.0%
	AACAAAGGTTACTACGGTCG+TGG	+	31046:81529-81548	MS.gene53961:CDS	45.0%
	AACTACAACTATGGACCAGC+TGG	+	31046:81571-81590	MS.gene53961:CDS	45.0%
	CAAAATTGTGGGTGTGCTGA+AGG	+	31046:80889-80908	MS.gene53961:CDS	45.0%
	CGACCGTAGTAACCTTTGTT+AGG	-	31046:81529-81548	None:intergenic	45.0%
	GAAGGACACAACAGCATCAT+TGG	-	31046:81641-81660	None:intergenic	45.0%
	GCATCATTGTTGGGCAAACT+TGG	-	31046:81009-81028	None:intergenic	45.0%
	GCCCAACAATGATGCTAATG+TGG	+	31046:81014-81033	MS.gene53961:CDS	45.0%
	GCCCCTAACAAAGGTTACTA+CGG	+	31046:81523-81542	MS.gene53961:CDS	45.0%
	GTTTGGTAGGTCTGGTACTT+TGG	+	31046:81155-81174	MS.gene53961:CDS	45.0%
	TAATGGTTGCACCAAAGCCT+TGG	-	31046:81711-81730	None:intergenic	45.0%
	TCATTGTTGGGCAAACTTGG+TGG	-	31046:81006-81025	None:intergenic	45.0%
	TTTGGATGACTCCAAACGTG+AGG	+	31046:81173-81192	MS.gene53961:CDS	45.0%
!	AAGGCTTTGGTGCAACCATT+AGG	+	31046:81710-81729	MS.gene53961:CDS	45.0%
!	AGGCTTTGGTGCAACCATTA+GGG	+	31046:81711-81730	MS.gene53961:CDS	45.0%
!	CAAGCTGATTCTAGCTGTAC+AGG	+	31046:81078-81097	MS.gene53961:CDS	45.0%
!	GTGGACCGATTCAACTATCT+TGG	+	31046:81548-81567	MS.gene53961:CDS	45.0%
!	TAACCTTTGTTAGGGGCACA+TGG	-	31046:81520-81539	None:intergenic	45.0%
!!	TATTAATTTATTATCAAATT+TGG	+	31046:81319-81338	MS.gene53961:intron	5.0%
	ACACGAGTCTGAACTGTGCT+TGG	-	31046:81772-81791	None:intergenic	50.0%
	AGCACAGTTCAGACTCGTGT+TGG	+	31046:81772-81791	MS.gene53961:CDS	50.0%
	CACGAGTCTGAACTGTGCTT+GGG	-	31046:81771-81790	None:intergenic	50.0%
	GAGTGTGTTGCAGCCAATAC+GGG	+	31046:80911-80930	MS.gene53961:CDS	50.0%
	GCATCATTGGCCACTGTTTC+AGG	-	31046:81628-81647	None:intergenic	50.0%
	GGAGTGTGTTGCAGCCAATA+CGG	+	31046:80910-80929	MS.gene53961:CDS	50.0%
	GGTGATGCATATTGTGGCAC+AGG	+	31046:80946-80965	MS.gene53961:CDS	50.0%
	GGTGTTTGGCCTGCATAACA+AGG	-	31046:80985-81004	None:intergenic	50.0%
	TGTGGCACAGGATGCAAACA+AGG	+	31046:80958-80977	MS.gene53961:CDS	50.0%
!	GGTGTCATCAACCAAGGCTT+TGG	+	31046:81697-81716	MS.gene53961:CDS	50.0%
!	TGCAGCCAATACGGGTATTG+TGG	+	31046:80919-80938	MS.gene53961:CDS	50.0%
!!	TGGGCAAACTTGGTGGTGTT+TGG	-	31046:80999-81018	None:intergenic	50.0%
	AGCAGCAGCAACCTCACGTT+TGG	-	31046:81187-81206	None:intergenic	55.0%
	GGACCAGCTGGAAGAGACAA+TGG	+	31046:81583-81602	MS.gene53961:CDS	55.0%
	TGGCCATGTGCCCCTAACAA+AGG	+	31046:81514-81533	MS.gene53961:CDS	55.0%
!!	AGTGAGTTGGGTGTTGCTCC+TGG	+	31046:81814-81833	MS.gene53961:CDS	55.0%
!!	GTGCACGGTGTCATCAACCA+AGG	+	31046:81691-81710	MS.gene53961:CDS	55.0%

Chromosome	Type	Strat	End	Strand	Name
31046	gene	80865	81855	80865	ID=MS.gene53961
31046	mRNA	80865	81855	80865	ID=MS.gene53961.t1;Parent=MS.gene53961
31046	exon	80865	81234	80865	ID=MS.gene53961.t1.exon1;Parent=MS.gene53961.t1
31046	CDS	80865	81234	80865	ID=cds.MS.gene53961.t1;Parent=MS.gene53961.t1
31046	exon	81449	81855	81449	ID=MS.gene53961.t1.exon2;Parent=MS.gene53961.t1
31046	CDS	81449	81855	81449	ID=cds.MS.gene53961.t1;Parent=MS.gene53961.t1

Gene Sequence

>MS.gene53961

ATGGTTCCAAAAAATGTTAGTGCTCAAAATTGTGGGTGTGCTGAAGGAGTGTGTTGCAGCCAATACGGGTATTGTGGTAATGGTGATGCATATTGTGGCACAGGATGCAAACAAGGTCCTTGTTATGCAGGCCAAACACCACCAAGTTTGCCCAACAATGATGCTAATGTGGCTGACATCCTTACACAAGATTTCTTCAATAGTATAATTGATCAAGCTGATTCTAGCTGTACAGGAAAGAACTTCTACACAAGAGCTGCTTTTCTTGATGCACTTAATTCTTATAATCAGTTTGGTAGGTCTGGTACTTTGGATGACTCCAAACGTGAGGTTGCTGCTGCTTTTGCTCATTTCACACATGAAACTGGACGTAAGTATTACGAATCATAATTTATCATTGTATTCAGTAACCATCAACTAAACAAAATTAACGATATATTCAAACTGTGTTAACTATTAATTTATTATCAAATTTGGTAACACAATTATTGGGTAAATAATTTCATGATTTTTAATCTCATATTCTCTTTTTACTAGGGACGTGTTTATGAACACACATAACATGTGATATTAATGTTGTGCAGATTTTTGCTATACTGAAGAGATTGATGGTCCATCAAAGGACTACTGTGATGAAAACAACACAGAGTGGCCATGTGCCCCTAACAAAGGTTACTACGGTCGTGGACCGATTCAACTATCTTGGAACTACAACTATGGACCAGCTGGAAGAGACAATGGTTTTGATGGATTGAACTCTCCTGAAACAGTGGCCAATGATGCTGTTGTGTCCTTCAAGACAGCATTATGGTATTGGATGAATAATGTGCACGGTGTCATCAACCAAGGCTTTGGTGCAACCATTAGGGCTATTAATGGTCGACTTGAATGTGATGGTGCTAACCCAAGCACAGTTCAGACTCGTGTTGGTTACTACACTCAATATTGTAGTGAGTTGGGTGTTGCTCCTGGTGATAATCTTACTTGCTAG

Protein sequence

>MS.gene53961.t1

MVPKNVSAQNCGCAEGVCCSQYGYCGNGDAYCGTGCKQGPCYAGQTPPSLPNNDANVADILTQDFFNSIIDQADSSCTGKNFYTRAAFLDALNSYNQFGRSGTLDDSKREVAAAFAHFTHETGHFCYTEEIDGPSKDYCDENNTEWPCAPNKGYYGRGPIQLSWNYNYGPAGRDNGFDGLNSPETVANDAVVSFKTALWYWMNNVHGVINQGFGATIRAINGRLECDGANPSTVQTRVGYYTQYCSELGVAPGDNLTC