AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene54154


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene54154.t1 MTR_5g072510 95.556 225 10 0 1 225 1 225 8.74e-129 362
MS.gene54154.t1 MTR_3g031830 75.000 180 41 2 3 178 4 183 1.40e-84 251
MS.gene54154.t1 MTR_4g094428 63.830 188 52 2 33 204 42 229 7.73e-82 244
MS.gene54154.t1 MTR_1g442860 80.000 125 25 0 37 161 65 189 6.10e-79 236
MS.gene54154.t1 MTR_1g075990 78.014 141 31 0 27 167 43 183 9.17e-76 227
MS.gene54154.t1 MTR_2g016110 67.550 151 48 1 20 170 19 168 1.60e-73 221
MS.gene54154.t1 MTR_2g076400 73.333 135 36 0 36 170 32 166 2.68e-72 218
MS.gene54154.t1 MTR_7g097030 79.104 134 28 0 34 167 61 194 3.51e-72 219
MS.gene54154.t1 MTR_1g069825 80.153 131 26 0 37 167 52 182 2.69e-71 217
MS.gene54154.t1 MTR_2g092950 72.441 127 35 0 34 160 20 146 4.95e-71 214
MS.gene54154.t1 MTR_4g079930 68.462 130 41 0 31 160 23 152 1.96e-70 213
MS.gene54154.t1 MTR_7g115700 67.568 148 42 4 36 180 26 170 4.75e-70 212
MS.gene54154.t1 MTR_1g080210 73.427 143 38 0 37 179 40 182 8.39e-69 209
MS.gene54154.t1 MTR_8g086350 72.800 125 34 0 36 160 30 154 2.80e-59 185
MS.gene54154.t1 MTR_1g114020 66.000 50 17 0 31 80 65 114 7.41e-20 83.6
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene54154.t1 AT1G07090 67.021 188 52 2 1 186 1 180 1.80e-89 262
MS.gene54154.t1 AT5G58500 67.403 181 43 3 35 214 17 182 2.54e-84 248
MS.gene54154.t1 AT2G31160 73.885 157 40 1 13 168 29 185 4.83e-74 224
MS.gene54154.t1 AT2G42610 72.794 136 37 0 34 169 22 157 6.24e-72 217
MS.gene54154.t1 AT2G42610 72.794 136 37 0 34 169 22 157 6.24e-72 217
MS.gene54154.t1 AT5G28490 71.053 152 39 1 15 166 8 154 1.56e-70 214
MS.gene54154.t1 AT3G23290 81.746 126 23 0 36 161 47 172 3.11e-70 213
MS.gene54154.t1 AT3G04510 76.154 130 31 0 37 166 33 162 6.50e-68 207
MS.gene54154.t1 AT1G78815 71.200 125 36 0 36 160 39 163 1.23e-67 206
MS.gene54154.t1 AT4G18610 67.460 126 40 1 36 160 36 161 1.31e-63 196
MS.gene54154.t1 AT1G16910 66.165 133 45 0 33 165 19 151 5.84e-63 194

Find 71 sgRNAs with CRISPR-Local

Find 80 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
ACGCTCTCATCGGACGTCTT+AGG 0.202990 5.4:-61628939 MS.gene54154:CDS
CTGAAGTATCTTGATCAATT+TGG 0.206713 5.4:-61629060 MS.gene54154:CDS
CGGCTTGGTGTTGGTGTTGC+TGG 0.267162 5.4:+61629192 None:intergenic
CTGCACAGTGCTAGGGTTAA+TGG 0.269012 5.4:+61629105 None:intergenic
GTGATAGGTTGTCCGTATTT+CGG 0.338189 5.4:-61629021 MS.gene54154:CDS
CTTCCCCACGCTTGCTTAAG+AGG 0.344190 5.4:+61628967 None:intergenic
CGCTCTCATCGGACGTCTTA+GGG 0.360799 5.4:-61628938 MS.gene54154:CDS
TGTGACGGTGGTAGAATTAT+TGG 0.361961 5.4:+61628657 None:intergenic
GTTGGTGTTGCTGGTGATGA+AGG 0.383238 5.4:+61629201 None:intergenic
GATTTATCTGAGGGAAGTTA+AGG 0.397608 5.4:-61628854 MS.gene54154:CDS
GATGGTAACACCGAGAGAAT+TGG 0.417561 5.4:-61628700 MS.gene54154:CDS
AAAACTAAGGTGCACGTGAT+AGG 0.426316 5.4:-61629036 MS.gene54154:CDS
ATAAGGAACTCCTCTAGCTT+TGG 0.428349 5.4:+61628822 None:intergenic
ACGTCTTAGGGCTGCTTTCG+AGG 0.442268 5.4:-61628926 MS.gene54154:CDS
AGTCACAAAAGCGACGAGAC+TGG 0.455576 5.4:-61629164 MS.gene54154:CDS
GCGCAAGGAGAAGGTGGGTT+AGG 0.457008 5.4:+61628994 None:intergenic
GTGCTCCACTGCACAGTGCT+AGG 0.462382 5.4:+61629097 None:intergenic
TGTGGTTACGTAGATATTGT+TGG 0.476151 5.4:+61629133 None:intergenic
GGTGACGGTGGTGGTATTGA+TGG 0.478331 5.4:-61628718 MS.gene54154:CDS
CTTACCGCCCGAGTTCCAAA+TGG 0.497179 5.4:+61628877 None:intergenic
TCGGCAATGGAAGCTGCATC+TGG 0.503318 5.4:-61629285 None:intergenic
ACCTATTGTTAGTGTTAGTA+CGG 0.504598 5.4:-61628635 MS.gene54154:CDS
AAACCCGAGAGTAATCCATT+TGG 0.505797 5.4:-61628892 MS.gene54154:CDS
GACTCGTAGCGGCTTGGTGT+TGG 0.507637 5.4:+61629183 None:intergenic
CATTTGGAACTCGGGCGGTA+AGG 0.509273 5.4:-61628876 MS.gene54154:CDS
GCCGTACTAACACTAACAAT+AGG 0.510181 5.4:+61628634 None:intergenic
GGGTTAGGTTGTCCGAAATA+CGG 0.513501 5.4:+61629009 None:intergenic
GTTCCAAATGGATTACTCTC+GGG 0.518325 5.4:+61628889 None:intergenic
GTTGCTGGTGATGAAGGTTG+TGG 0.519042 5.4:+61629207 None:intergenic
AGTTCCAAATGGATTACTCT+CGG 0.520097 5.4:+61628888 None:intergenic
TGCTCCACTGCACAGTGCTA+GGG 0.520641 5.4:+61629098 None:intergenic
CTTGTCCTCTTAAGCAAGCG+TGG 0.521556 5.4:-61628972 MS.gene54154:CDS
AGGGCTGCTTTCGAGGAAAA+CGG 0.523449 5.4:-61628919 MS.gene54154:CDS
AGAGGACAAGCGCAAGGAGA+AGG 0.525742 5.4:+61628985 None:intergenic
GGTTGTGGTTGTGCTGCCTC+CGG 0.526300 5.4:+61629222 None:intergenic
GAGTAATCCATTTGGAACTC+GGG 0.526440 5.4:-61628884 MS.gene54154:CDS
AGAGTAATCCATTTGGAACT+CGG 0.528993 5.4:-61628885 MS.gene54154:CDS
GGACAAGCGCAAGGAGAAGG+TGG 0.534211 5.4:+61628988 None:intergenic
TTTGATGGTATTGACGGTGG+TGG 0.538750 5.4:+61629252 None:intergenic
GATGGTATTGACGGTGGTGG+TGG 0.541936 5.4:+61629255 None:intergenic
CTAGGGTTAATGGTGGTTTG+TGG 0.548564 5.4:+61629115 None:intergenic
GCGGTGAATAGTAGTGGACT+TGG 0.557889 5.4:-61628739 MS.gene54154:CDS
TTGTCCTCTTAAGCAAGCGT+GGG 0.563836 5.4:-61628971 MS.gene54154:CDS
AACACCAGCTCCAATTCTCT+CGG 0.568073 5.4:+61628690 None:intergenic
GGAAGTCTTGACGCTCTCAT+CGG 0.568235 5.4:-61628949 MS.gene54154:CDS
AGTGGACTTGGTGACGGTGG+TGG 0.574244 5.4:-61628727 MS.gene54154:CDS
GGCGGTAAGGATTTATCTGA+GGG 0.577139 5.4:-61628863 MS.gene54154:CDS
GGGCGGTAAGGATTTATCTG+AGG 0.579521 5.4:-61628864 MS.gene54154:CDS
TGTGGTTGTGCTGCCTCCGG+TGG 0.598177 5.4:+61629225 None:intergenic
ATTGCAGCAGCGGTGACAGT+GGG 0.601286 5.4:+61628778 None:intergenic
AACACTAACAATAGGTGTGA+CGG 0.607101 5.4:+61628642 None:intergenic
GCAATGGAAGCTGCATCTGG+TGG 0.607187 5.4:-61629282 MS.gene54154:CDS
TATTGCAGCAGCGGTGACAG+TGG 0.608654 5.4:+61628777 None:intergenic
GATGGTCAAGCCAAAGCTAG+AGG 0.608868 5.4:-61628832 MS.gene54154:CDS
TATCTGAGGGAAGTTAAGGA+TGG 0.611775 5.4:-61628850 MS.gene54154:CDS
GACAAGCGCAAGGAGAAGGT+GGG 0.614541 5.4:+61628989 None:intergenic
ACAGCAGCGGTGAATAGTAG+TGG 0.615069 5.4:-61628745 MS.gene54154:CDS
GAGAGAATTGGAGCTGGTGT+TGG 0.623654 5.4:-61628688 MS.gene54154:CDS
AGTTGATATTATTGCAGCAG+CGG 0.629514 5.4:+61628768 None:intergenic
GACGGTGGTAGAATTATTGG+TGG 0.631458 5.4:+61628660 None:intergenic
CACAGTGCTAGGGTTAATGG+TGG 0.634530 5.4:+61629108 None:intergenic
AATAGTAGTGGACTTGGTGA+CGG 0.643866 5.4:-61628733 MS.gene54154:CDS
TGCTTAAGAGGACAAGCGCA+AGG 0.646571 5.4:+61628979 None:intergenic
AACACCGAGAGAATTGGAGC+TGG 0.649987 5.4:-61628694 MS.gene54154:CDS
TTAACCCTAGCACTGTGCAG+TGG 0.666567 5.4:-61629102 MS.gene54154:CDS
AATAATATCAACTACAGCAG+CGG 0.669859 5.4:-61628758 MS.gene54154:CDS
TAATCCATTTGGAACTCGGG+CGG 0.682868 5.4:-61628881 MS.gene54154:CDS
AGTAGTGGACTTGGTGACGG+TGG 0.690950 5.4:-61628730 MS.gene54154:CDS
TGTCCTCTTAAGCAAGCGTG+GGG 0.708587 5.4:-61628970 MS.gene54154:CDS
ACTAACAATAGGTGTGACGG+TGG 0.726512 5.4:+61628645 None:intergenic
GCTGCTTTCGAGGAAAACGG+TGG 0.733556 5.4:-61628916 MS.gene54154:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
! TGATCAATTTGGAAAAACTA+AGG - chr5.4:61628854-61628873 MS.gene54154:CDS 25.0%
! TTCCTTATGAGAAAAAGAAA+CGG - chr5.4:61629096-61629115 MS.gene54154:CDS 25.0%
AATAATATCAACTACAGCAG+CGG - chr5.4:61629145-61629164 MS.gene54154:CDS 30.0%
ACCTATTGTTAGTGTTAGTA+CGG - chr5.4:61629268-61629287 MS.gene54154:CDS 30.0%
! CTGAAGTATCTTGATCAATT+TGG - chr5.4:61628843-61628862 MS.gene54154:CDS 30.0%
! TTCCGTTTCTTTTTCTCATA+AGG + chr5.4:61629101-61629120 None:intergenic 30.0%
AACACTAACAATAGGTGTGA+CGG + chr5.4:61629264-61629283 None:intergenic 35.0%
AATACCATCAAAAACACCAC+CGG - chr5.4:61628662-61628681 MS.gene54154:CDS 35.0%
ATGAGAAAAAGAAACGGAAG+AGG - chr5.4:61629102-61629121 MS.gene54154:CDS 35.0%
GATTTATCTGAGGGAAGTTA+AGG - chr5.4:61629049-61629068 MS.gene54154:CDS 35.0%
TGTGGTTACGTAGATATTGT+TGG + chr5.4:61628773-61628792 None:intergenic 35.0%
! AGAGTAATCCATTTGGAACT+CGG - chr5.4:61629018-61629037 MS.gene54154:CDS 35.0%
! AGTTGATATTATTGCAGCAG+CGG + chr5.4:61629138-61629157 None:intergenic 35.0%
!! AGTTCCAAATGGATTACTCT+CGG + chr5.4:61629018-61629037 None:intergenic 35.0%
!! GGTGTTTTTGATGGTATTGA+CGG + chr5.4:61628660-61628679 None:intergenic 35.0%
AAAACTAAGGTGCACGTGAT+AGG - chr5.4:61628867-61628886 MS.gene54154:CDS 40.0%
AAACCCGAGAGTAATCCATT+TGG - chr5.4:61629011-61629030 MS.gene54154:CDS 40.0%
GCCGTACTAACACTAACAAT+AGG + chr5.4:61629272-61629291 None:intergenic 40.0%
GTGATAGGTTGTCCGTATTT+CGG - chr5.4:61628882-61628901 MS.gene54154:CDS 40.0%
TATCTGAGGGAAGTTAAGGA+TGG - chr5.4:61629053-61629072 MS.gene54154:CDS 40.0%
TGTGACGGTGGTAGAATTAT+TGG + chr5.4:61629249-61629268 None:intergenic 40.0%
! ATAAGGAACTCCTCTAGCTT+TGG + chr5.4:61629084-61629103 None:intergenic 40.0%
! GAGTAATCCATTTGGAACTC+GGG - chr5.4:61629019-61629038 MS.gene54154:CDS 40.0%
!! AATAGTAGTGGACTTGGTGA+CGG - chr5.4:61629170-61629189 MS.gene54154:CDS 40.0%
!! GTTCCAAATGGATTACTCTC+GGG + chr5.4:61629017-61629036 None:intergenic 40.0%
!!! GTTTTTGATGGTATTGACGG+TGG + chr5.4:61628657-61628676 None:intergenic 40.0%
AACACCAGCTCCAATTCTCT+CGG + chr5.4:61629216-61629235 None:intergenic 45.0%
ACTAACAATAGGTGTGACGG+TGG + chr5.4:61629261-61629280 None:intergenic 45.0%
CTAGGGTTAATGGTGGTTTG+TGG + chr5.4:61628791-61628810 None:intergenic 45.0%
GACGGTGGTAGAATTATTGG+TGG + chr5.4:61629246-61629265 None:intergenic 45.0%
GATGGTAACACCGAGAGAAT+TGG - chr5.4:61629203-61629222 MS.gene54154:CDS 45.0%
GGCGGTAAGGATTTATCTGA+GGG - chr5.4:61629040-61629059 MS.gene54154:CDS 45.0%
TTGTCCTCTTAAGCAAGCGT+GGG - chr5.4:61628932-61628951 MS.gene54154:CDS 45.0%
! GGGTTAGGTTGTCCGAAATA+CGG + chr5.4:61628897-61628916 None:intergenic 45.0%
! TAATCCATTTGGAACTCGGG+CGG - chr5.4:61629022-61629041 MS.gene54154:CDS 45.0%
!! TTTGATGGTATTGACGGTGG+TGG + chr5.4:61628654-61628673 None:intergenic 45.0%
AACACCGAGAGAATTGGAGC+TGG - chr5.4:61629209-61629228 MS.gene54154:CDS 50.0%
ACAGCAGCGGTGAATAGTAG+TGG - chr5.4:61629158-61629177 MS.gene54154:CDS 50.0%
ACCATCAAAAACACCACCGG+AGG - chr5.4:61628665-61628684 MS.gene54154:CDS 50.0%
AGTCACAAAAGCGACGAGAC+TGG - chr5.4:61628739-61628758 MS.gene54154:CDS 50.0%
CACAGTGCTAGGGTTAATGG+TGG + chr5.4:61628798-61628817 None:intergenic 50.0%
CTGCACAGTGCTAGGGTTAA+TGG + chr5.4:61628801-61628820 None:intergenic 50.0%
CTTGTCCTCTTAAGCAAGCG+TGG - chr5.4:61628931-61628950 MS.gene54154:CDS 50.0%
GAGAGAATTGGAGCTGGTGT+TGG - chr5.4:61629215-61629234 MS.gene54154:CDS 50.0%
GATGGTCAAGCCAAAGCTAG+AGG - chr5.4:61629071-61629090 MS.gene54154:CDS 50.0%
GGAAGTCTTGACGCTCTCAT+CGG - chr5.4:61628954-61628973 MS.gene54154:CDS 50.0%
GGGCGGTAAGGATTTATCTG+AGG - chr5.4:61629039-61629058 MS.gene54154:CDS 50.0%
TGCTTAAGAGGACAAGCGCA+AGG + chr5.4:61628927-61628946 None:intergenic 50.0%
TGTCCTCTTAAGCAAGCGTG+GGG - chr5.4:61628933-61628952 MS.gene54154:CDS 50.0%
TTAACCCTAGCACTGTGCAG+TGG - chr5.4:61628801-61628820 MS.gene54154:CDS 50.0%
! AGGGCTGCTTTCGAGGAAAA+CGG - chr5.4:61628984-61629003 MS.gene54154:CDS 50.0%
! GTTGCTGGTGATGAAGGTTG+TGG + chr5.4:61628699-61628718 None:intergenic 50.0%
! TTTTGTGACTCGTAGCGGCT+TGG + chr5.4:61628729-61628748 None:intergenic 50.0%
!! GCGGTGAATAGTAGTGGACT+TGG - chr5.4:61629164-61629183 MS.gene54154:CDS 50.0%
!! GTTGGTGTTGCTGGTGATGA+AGG + chr5.4:61628705-61628724 None:intergenic 50.0%
!!! GTCGCTTTTGTGACTCGTAG+CGG + chr5.4:61628734-61628753 None:intergenic 50.0%
ACGCTCTCATCGGACGTCTT+AGG - chr5.4:61628964-61628983 MS.gene54154:CDS 55.0%
AGAGGACAAGCGCAAGGAGA+AGG + chr5.4:61628921-61628940 None:intergenic 55.0%
ATTGCAGCAGCGGTGACAGT+GGG + chr5.4:61629128-61629147 None:intergenic 55.0%
CGCTCTCATCGGACGTCTTA+GGG - chr5.4:61628965-61628984 MS.gene54154:CDS 55.0%
CTTACCGCCCGAGTTCCAAA+TGG + chr5.4:61629029-61629048 None:intergenic 55.0%
CTTCCCCACGCTTGCTTAAG+AGG + chr5.4:61628939-61628958 None:intergenic 55.0%
GACAAGCGCAAGGAGAAGGT+GGG + chr5.4:61628917-61628936 None:intergenic 55.0%
TATTGCAGCAGCGGTGACAG+TGG + chr5.4:61629129-61629148 None:intergenic 55.0%
TGCTCCACTGCACAGTGCTA+GGG + chr5.4:61628808-61628827 None:intergenic 55.0%
! ACGTCTTAGGGCTGCTTTCG+AGG - chr5.4:61628977-61628996 MS.gene54154:CDS 55.0%
! CATTTGGAACTCGGGCGGTA+AGG - chr5.4:61629027-61629046 MS.gene54154:CDS 55.0%
! GCTGCTTTCGAGGAAAACGG+TGG - chr5.4:61628987-61629006 MS.gene54154:CDS 55.0%
! GGTGACGGTGGTGGTATTGA+TGG - chr5.4:61629185-61629204 MS.gene54154:CDS 55.0%
!! AGTAGTGGACTTGGTGACGG+TGG - chr5.4:61629173-61629192 MS.gene54154:CDS 55.0%
!! GATGGTATTGACGGTGGTGG+TGG + chr5.4:61628651-61628670 None:intergenic 55.0%
!! GCCTCCGGTGGTGTTTTTGA+TGG + chr5.4:61628669-61628688 None:intergenic 55.0%
GGACAAGCGCAAGGAGAAGG+TGG + chr5.4:61628918-61628937 None:intergenic 60.0%
GGTTGTGGTTGTGCTGCCTC+CGG + chr5.4:61628684-61628703 None:intergenic 60.0%
GTGCTCCACTGCACAGTGCT+AGG + chr5.4:61628809-61628828 None:intergenic 60.0%
! GCGCAAGGAGAAGGTGGGTT+AGG + chr5.4:61628912-61628931 None:intergenic 60.0%
!! AGTGGACTTGGTGACGGTGG+TGG - chr5.4:61629176-61629195 MS.gene54154:CDS 60.0%
!! CGGCTTGGTGTTGGTGTTGC+TGG + chr5.4:61628714-61628733 None:intergenic 60.0%
!! GACTCGTAGCGGCTTGGTGT+TGG + chr5.4:61628723-61628742 None:intergenic 60.0%
TGTGGTTGTGCTGCCTCCGG+TGG + chr5.4:61628681-61628700 None:intergenic 65.0%


Chromosome Type Strat End Strand Name
chr5.4 gene 61628624 61629301 61628624 ID=MS.gene54154
chr5.4 mRNA 61628624 61629301 61628624 ID=MS.gene54154.t1;Parent=MS.gene54154
chr5.4 exon 61628624 61629301 61628624 ID=MS.gene54154.t1.exon1;Parent=MS.gene54154.t1
chr5.4 CDS 61628624 61629301 61628624 ID=cds.MS.gene54154.t1;Parent=MS.gene54154.t1
Gene Sequence

>MS.gene54154

ATGGAAGCTGCATCTGGTGGAGAACCACCACCACCGTCAATACCATCAAAAACACCACCGGAGGCAGCACAACCACAACCTTCATCACCAGCAACACCAACACCAAGCCGCTACGAGTCACAAAAGCGACGAGACTGGAACACGTTCCAACAATATCTACGTAACCACAAACCACCATTAACCCTAGCACTGTGCAGTGGAGCACACGTGATCGAGTTTCTGAAGTATCTTGATCAATTTGGAAAAACTAAGGTGCACGTGATAGGTTGTCCGTATTTCGGACAACCTAACCCACCTTCTCCTTGCGCTTGTCCTCTTAAGCAAGCGTGGGGAAGTCTTGACGCTCTCATCGGACGTCTTAGGGCTGCTTTCGAGGAAAACGGTGGTAAACCCGAGAGTAATCCATTTGGAACTCGGGCGGTAAGGATTTATCTGAGGGAAGTTAAGGATGGTCAAGCCAAAGCTAGAGGAGTTCCTTATGAGAAAAAGAAACGGAAGAGGCCCACTGTCACCGCTGCTGCAATAATATCAACTACAGCAGCGGTGAATAGTAGTGGACTTGGTGACGGTGGTGGTATTGATGGTAACACCGAGAGAATTGGAGCTGGTGTTGGTGTTACCACCAATAATTCTACCACCGTCACACCTATTGTTAGTGTTAGTACGGCTACAGTATAG

Protein sequence

>MS.gene54154.t1

MEAASGGEPPPPSIPSKTPPEAAQPQPSSPATPTPSRYESQKRRDWNTFQQYLRNHKPPLTLALCSGAHVIEFLKYLDQFGKTKVHVIGCPYFGQPNPPSPCACPLKQAWGSLDALIGRLRAAFEENGGKPESNPFGTRAVRIYLREVKDGQAKARGVPYEKKKRKRPTVTAAAIISTTAAVNSSGLGDGGGIDGNTERIGAGVGVTTNNSTTVTPIVSVSTATV