Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene54341.t1 | PNY16455.1 | 92.3 | 39 | 3 | 0 | 6 | 44 | 390 | 428 | 1.30E-10 | 75.5 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene54341.t1 | F2Y4A3 | 59.0 | 39 | 16 | 0 | 6 | 44 | 381 | 419 | 2.1e-06 | 52.8 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene54341.t1 | A0A2K3PME8 | 92.3 | 39 | 3 | 0 | 6 | 44 | 390 | 428 | 9.2e-11 | 75.5 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene051181 | MS.gene54341 | 0.800211 | 1.60E-48 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Find 17 sgRNAs with CRISPR-Local
Find 47 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| TCTCCGATGGTTATCTTATC+TGG | 0.264805 | 7.3:+82150941 | MS.gene54341:CDS |
| AATGATGTCAGAGCTGAATA+AGG | 0.325937 | 7.3:+82151007 | MS.gene54341:CDS |
| ATCGAAAAGACCTCAGAAAA+AGG | 0.380789 | 7.3:+82151823 | MS.gene54341:CDS |
| TGAGAGTGAGAAGGAGAGTG+AGG | 0.458055 | 7.3:+82151793 | MS.gene54341:CDS |
| TTGACAACAAATGTAACTTC+GGG | 0.505763 | 7.3:-82150986 | None:intergenic |
| ATTGACAACAAATGTAACTT+CGG | 0.507378 | 7.3:-82150987 | None:intergenic |
| TGCGTATGTCACATACCTAA+AGG | 0.519525 | 7.3:+82151046 | MS.gene54341:CDS |
| CTTTAGGTGTGGTCATTGCA+AGG | 0.538726 | 7.3:+82152023 | MS.gene54341:intron |
| ATGATGTCAGAGCTGAATAA+GGG | 0.543037 | 7.3:+82151008 | MS.gene54341:CDS |
| TGGTAAATGCAGTTCTCCGA+TGG | 0.552479 | 7.3:+82150928 | None:intergenic |
| CGACTCTAATGAGAGTGAGA+AGG | 0.614895 | 7.3:+82151784 | MS.gene54341:CDS |
| AGTCCAGATAAGATAACCAT+CGG | 0.618277 | 7.3:-82150944 | None:intergenic |
| CTTCTCACTCTCATTAGAGT+CGG | 0.624945 | 7.3:-82151783 | None:intergenic |
| AGAACATCCTTACATAAGCA+AGG | 0.627518 | 7.3:-82152047 | None:intergenic |
| TGTCAGAGCTGAATAAGGGA+GGG | 0.635123 | 7.3:+82151012 | MS.gene54341:CDS |
| ATGTCAGAGCTGAATAAGGG+AGG | 0.639595 | 7.3:+82151011 | MS.gene54341:CDS |
| GGATAAGATGAATGTTGACA+AGG | 0.656941 | 7.3:+82151754 | MS.gene54341:intron |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | TATATATATATAATTGAAAC+CGG | - | chr7.3:82151674-82151693 | None:intergenic | 10.0% |
| !!! | TTTTAACAAAAAATTTGCTT+TGG | + | chr7.3:82151905-82151924 | MS.gene54341:intron | 15.0% |
| !! | ATGATTCTTATAGATCATTT+GGG | + | chr7.3:82151494-82151513 | MS.gene54341:intron | 20.0% |
| !! | ATTCAAACATGCATTAAAAT+TGG | + | chr7.3:82151289-82151308 | MS.gene54341:intron | 20.0% |
| !! | TGTGGATATATTTATATGTT+GGG | + | chr7.3:82151563-82151582 | MS.gene54341:intron | 20.0% |
| !! | TTGTGGATATATTTATATGT+TGG | + | chr7.3:82151562-82151581 | MS.gene54341:intron | 20.0% |
| !!! | CTTTGATTTTGATTTTAGTT+TGG | + | chr7.3:82151875-82151894 | MS.gene54341:intron | 20.0% |
| !!! | TTCATTGTTTTATGTCAATT+TGG | + | chr7.3:82151385-82151404 | MS.gene54341:intron | 20.0% |
| ! | AATTCACTGCTATTATCTTT+AGG | + | chr7.3:82152007-82152026 | MS.gene54341:intron | 25.0% |
| ! | ATTGACAACAAATGTAACTT+CGG | - | chr7.3:82150990-82151009 | None:intergenic | 25.0% |
| ! | CTGTTAAGATTGTAAAATCA+TGG | + | chr7.3:82151152-82151171 | MS.gene54341:intron | 25.0% |
| ! | GAATGATATGTTGATATGTT+GGG | + | chr7.3:82151635-82151654 | MS.gene54341:intron | 25.0% |
| ! | GATGATTCTTATAGATCATT+TGG | + | chr7.3:82151493-82151512 | MS.gene54341:intron | 25.0% |
| ! | TGAATGATATGTTGATATGT+TGG | + | chr7.3:82151634-82151653 | MS.gene54341:intron | 25.0% |
| ! | TGATTCTTATAGATCATTTG+GGG | + | chr7.3:82151495-82151514 | MS.gene54341:intron | 25.0% |
| !! | TAAAACTTTTCGTAATTGAC+AGG | + | chr7.3:82151733-82151752 | MS.gene54341:intron | 25.0% |
| !!! | AACTCACATTTTCATTTTTG+CGG | - | chr7.3:82151363-82151382 | None:intergenic | 25.0% |
| !!! | CTAGCTTTATTTCTTAATGT+AGG | + | chr7.3:82151432-82151451 | MS.gene54341:intron | 25.0% |
| AATGTGATGAAAGATTCTTG+TGG | + | chr7.3:82151545-82151564 | MS.gene54341:intron | 30.0% | |
| ACTGTAAAATTTGTGAGTGT+TGG | + | chr7.3:82151604-82151623 | MS.gene54341:intron | 30.0% | |
| GTTGGGAACTATTTATATTC+CGG | + | chr7.3:82151652-82151671 | MS.gene54341:intron | 30.0% | |
| TATCAGAAAATCACACCTTT+AGG | - | chr7.3:82151064-82151083 | None:intergenic | 30.0% | |
| TCTAATAATAGCTGTGTGTT+TGG | + | chr7.3:82151945-82151964 | MS.gene54341:intron | 30.0% | |
| TTGACAACAAATGTAACTTC+GGG | - | chr7.3:82150989-82151008 | None:intergenic | 30.0% | |
| AATGATGTCAGAGCTGAATA+AGG | + | chr7.3:82151007-82151026 | MS.gene54341:CDS | 35.0% | |
| ACTGCTATTATCTTTAGGTG+TGG | + | chr7.3:82152012-82152031 | MS.gene54341:intron | 35.0% | |
| AGTCCAGATAAGATAACCAT+CGG | - | chr7.3:82150947-82150966 | None:intergenic | 35.0% | |
| ATCGAAAAGACCTCAGAAAA+AGG | + | chr7.3:82151823-82151842 | MS.gene54341:CDS | 35.0% | |
| ATGATGTCAGAGCTGAATAA+GGG | + | chr7.3:82151008-82151027 | MS.gene54341:CDS | 35.0% | |
| GGATAAGATGAATGTTGACA+AGG | + | chr7.3:82151754-82151773 | MS.gene54341:intron | 35.0% | |
| ! | TAATTAGAGTACCTTTGCGA+AGG | + | chr7.3:82151327-82151346 | MS.gene54341:intron | 35.0% |
| ! | TATATGCTGACCTTTTTCTG+AGG | - | chr7.3:82151836-82151855 | None:intergenic | 35.0% |
| !! | ATTGAGCTTTGAAAGAGTCT+TGG | + | chr7.3:82151112-82151131 | MS.gene54341:intron | 35.0% |
| !! | TTTCTGAGTGTTTTGTTACG+TGG | + | chr7.3:82151471-82151490 | MS.gene54341:intron | 35.0% |
| !!! | TTTTTCTGAGGTCTTTTCGA+TGG | - | chr7.3:82151824-82151843 | None:intergenic | 35.0% |
| CTTCTCACTCTCATTAGAGT+CGG | - | chr7.3:82151786-82151805 | None:intergenic | 40.0% | |
| TCTCCGATGGTTATCTTATC+TGG | + | chr7.3:82150941-82150960 | MS.gene54341:CDS | 40.0% | |
| TGCGTATGTCACATACCTAA+AGG | + | chr7.3:82151046-82151065 | MS.gene54341:CDS | 40.0% | |
| ! | AAATTCAGCACTGAACAGAC+AGG | + | chr7.3:82151520-82151539 | MS.gene54341:intron | 40.0% |
| ! | GCTGTGTGTTTGGAGTAAAA+AGG | + | chr7.3:82151955-82151974 | MS.gene54341:intron | 40.0% |
| ATGTCAGAGCTGAATAAGGG+AGG | + | chr7.3:82151011-82151030 | MS.gene54341:CDS | 45.0% | |
| CGACTCTAATGAGAGTGAGA+AGG | + | chr7.3:82151784-82151803 | MS.gene54341:CDS | 45.0% | |
| CTTTAGGTGTGGTCATTGCA+AGG | + | chr7.3:82152023-82152042 | MS.gene54341:intron | 45.0% | |
| GAAAGTAACCTCCTTCGCAA+AGG | - | chr7.3:82151341-82151360 | None:intergenic | 45.0% | |
| TGTCAGAGCTGAATAAGGGA+GGG | + | chr7.3:82151012-82151031 | MS.gene54341:CDS | 45.0% | |
| ! | TTAGAGTACCTTTGCGAAGG+AGG | + | chr7.3:82151330-82151349 | MS.gene54341:intron | 45.0% |
| TGAGAGTGAGAAGGAGAGTG+AGG | + | chr7.3:82151793-82151812 | MS.gene54341:CDS | 50.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr7.3 | gene | 82150940 | 82152056 | 82150940 | ID=MS.gene54341 |
| chr7.3 | mRNA | 82150940 | 82152056 | 82150940 | ID=MS.gene54341.t1;Parent=MS.gene54341 |
| chr7.3 | exon | 82150940 | 82151067 | 82150940 | ID=MS.gene54341.t1.exon1;Parent=MS.gene54341.t1 |
| chr7.3 | CDS | 82150940 | 82151067 | 82150940 | ID=cds.MS.gene54341.t1;Parent=MS.gene54341.t1 |
| chr7.3 | exon | 82151755 | 82151844 | 82151755 | ID=MS.gene54341.t1.exon2;Parent=MS.gene54341.t1 |
| chr7.3 | CDS | 82151755 | 82151844 | 82151755 | ID=cds.MS.gene54341.t1;Parent=MS.gene54341.t1 |
| chr7.3 | exon | 82152029 | 82152056 | 82152029 | ID=MS.gene54341.t1.exon3;Parent=MS.gene54341.t1 |
| chr7.3 | CDS | 82152029 | 82152056 | 82152029 | ID=cds.MS.gene54341.t1;Parent=MS.gene54341.t1 |
| Gene Sequence |
| Protein sequence |
