Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene54342.t1 | XP_013469889.1 | 93.8 | 65 | 4 | 0 | 1 | 65 | 149 | 213 | 1.20E-22 | 115.2 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene54342.t1 | Q8GUN6 | 60.0 | 65 | 23 | 1 | 1 | 65 | 148 | 209 | 2.5e-13 | 75.5 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene54342.t1 | A0A072VQ71 | 93.8 | 65 | 4 | 0 | 1 | 65 | 149 | 213 | 8.4e-23 | 115.2 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene049744 | MS.gene54342 | 0.805704 | 1.17E-49 | -1.69E-46 |
| MS.gene054479 | MS.gene54342 | 0.811925 | 5.49E-51 | -1.69E-46 |
| MS.gene05595 | MS.gene54342 | 0.818841 | 1.59E-52 | -1.69E-46 |
| MS.gene056173 | MS.gene54342 | 0.813948 | 1.98E-51 | -1.69E-46 |
| MS.gene060011 | MS.gene54342 | 0.830407 | 2.99E-55 | -1.69E-46 |
| MS.gene06052 | MS.gene54342 | 0.800453 | 1.43E-48 | -1.69E-46 |
| MS.gene060998 | MS.gene54342 | 0.805175 | 1.52E-49 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene54342.t1 | MTR_1g105540 | 93.846 | 65 | 4 | 0 | 1 | 65 | 149 | 213 | 3.23e-36 | 123 |
| MS.gene54342.t1 | MTR_8g464090 | 92.105 | 38 | 2 | 1 | 1 | 38 | 80 | 116 | 3.35e-16 | 68.9 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene54342.t1 | AT1G61770 | 60.938 | 64 | 22 | 1 | 1 | 64 | 148 | 208 | 4.78e-20 | 80.9 |
Find 20 sgRNAs with CRISPR-Local
Find 27 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| CAAAGTCCAAACCTTATATA+TGG | 0.338721 | 7.3:-82147627 | None:intergenic |
| GAAGAAGGGTCAAAAGAATA+AGG | 0.362359 | 7.3:+82147357 | MS.gene54342:CDS |
| TGAAAGGCCCTCCATATATA+AGG | 0.367855 | 7.3:+82147616 | MS.gene54342:CDS |
| GAACTTGATCTGCAGATAAC+AGG | 0.414089 | 7.3:+82147590 | MS.gene54342:CDS |
| TTGGAGCTTGAGCGCAATGG+TGG | 0.491453 | 7.3:+82147322 | MS.gene54342:CDS |
| GTTAATTAAGGCTGTGGCCA+TGG | 0.502580 | 7.3:+82147261 | None:intergenic |
| GACCTTTATATACAGAAAAG+TGG | 0.524010 | 7.3:+82147541 | MS.gene54342:intron |
| TGGGATTACAAATACGAAGA+AGG | 0.536769 | 7.3:+82147342 | MS.gene54342:CDS |
| GGAGGAAGACCTCAGTAAAG+TGG | 0.539095 | 7.3:+82147562 | MS.gene54342:CDS |
| AGGGTCAAAAGAATAAGGAC+AGG | 0.571889 | 7.3:+82147362 | MS.gene54342:CDS |
| GTCCAAACCTTATATATGGA+GGG | 0.582016 | 7.3:-82147623 | None:intergenic |
| GGGATTACAAATACGAAGAA+GGG | 0.592260 | 7.3:+82147343 | MS.gene54342:CDS |
| AGTCCAAACCTTATATATGG+AGG | 0.596412 | 7.3:-82147624 | None:intergenic |
| TGGAGCTTGAGCGCAATGGT+GGG | 0.598174 | 7.3:+82147323 | MS.gene54342:CDS |
| TGCAGATAACAGGAACTGAA+AGG | 0.599633 | 7.3:+82147600 | MS.gene54342:CDS |
| GTTCTTCCTCCACTTTACTG+AGG | 0.607572 | 7.3:-82147571 | None:intergenic |
| GGAAGACCTCAGTAAAGTGG+AGG | 0.640383 | 7.3:+82147565 | MS.gene54342:CDS |
| GCGTTGGAGCTTGAGCGCAA+TGG | 0.658338 | 7.3:+82147319 | MS.gene54342:CDS |
| AGCAGATGTCTTCTTCACCA+TGG | 0.671975 | 7.3:-82147278 | None:intergenic |
| CTTTATATACAGAAAAGTGG+AGG | 0.682566 | 7.3:+82147544 | MS.gene54342:intron |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | TGCTTATAAAAATAAACTAA+GGG | + | chr7.3:82147297-82147316 | MS.gene54342:CDS | 15.0% |
| !! | ATCAAAAGTAAAGAAATTAC+AGG | - | chr7.3:82147500-82147519 | None:intergenic | 20.0% |
| !! | CTGCTTATAAAAATAAACTA+AGG | + | chr7.3:82147296-82147315 | MS.gene54342:CDS | 20.0% |
| ! | TCTTATGAATCAAAGAGAAA+CGG | - | chr7.3:82147464-82147483 | None:intergenic | 25.0% |
| CTTATGAATCAAAGAGAAAC+GGG | - | chr7.3:82147463-82147482 | None:intergenic | 30.0% | |
| CTTTATATACAGAAAAGTGG+AGG | + | chr7.3:82147544-82147563 | MS.gene54342:intron | 30.0% | |
| GACCTTTATATACAGAAAAG+TGG | + | chr7.3:82147541-82147560 | MS.gene54342:intron | 30.0% | |
| ! | TAAAAATAAACTAAGGGCGT+TGG | + | chr7.3:82147303-82147322 | MS.gene54342:CDS | 30.0% |
| !! | CTCCACTTTTCTGTATATAA+AGG | - | chr7.3:82147546-82147565 | None:intergenic | 30.0% |
| AAGGAGGAAAATTACACACA+CGG | - | chr7.3:82147415-82147434 | None:intergenic | 35.0% | |
| AGACGAAATGAAAGTTTGCA+AGG | - | chr7.3:82147434-82147453 | None:intergenic | 35.0% | |
| CAAAAGAATAAGGACAGGTA+TGG | + | chr7.3:82147367-82147386 | MS.gene54342:intron | 35.0% | |
| GAAGAAGGGTCAAAAGAATA+AGG | + | chr7.3:82147357-82147376 | MS.gene54342:CDS | 35.0% | |
| GGGATTACAAATACGAAGAA+GGG | + | chr7.3:82147343-82147362 | MS.gene54342:CDS | 35.0% | |
| TGGGATTACAAATACGAAGA+AGG | + | chr7.3:82147342-82147361 | MS.gene54342:CDS | 35.0% | |
| AGGGTCAAAAGAATAAGGAC+AGG | + | chr7.3:82147362-82147381 | MS.gene54342:CDS | 40.0% | |
| CGAAATGAAAGTTTGCAAGG+AGG | - | chr7.3:82147431-82147450 | None:intergenic | 40.0% | |
| GAACTTGATCTGCAGATAAC+AGG | + | chr7.3:82147590-82147609 | MS.gene54342:CDS | 40.0% | |
| TGAAAGGCCCTCCATATATA+AGG | + | chr7.3:82147616-82147635 | MS.gene54342:CDS | 40.0% | |
| TGCAGATAACAGGAACTGAA+AGG | + | chr7.3:82147600-82147619 | MS.gene54342:CDS | 40.0% | |
| AGCAGATGTCTTCTTCACCA+TGG | - | chr7.3:82147281-82147300 | None:intergenic | 45.0% | |
| GTTCTTCCTCCACTTTACTG+AGG | - | chr7.3:82147574-82147593 | None:intergenic | 45.0% | |
| GGAAGACCTCAGTAAAGTGG+AGG | + | chr7.3:82147565-82147584 | MS.gene54342:CDS | 50.0% | |
| GGAGGAAGACCTCAGTAAAG+TGG | + | chr7.3:82147562-82147581 | MS.gene54342:CDS | 50.0% | |
| TGGAGCTTGAGCGCAATGGT+GGG | + | chr7.3:82147323-82147342 | MS.gene54342:CDS | 55.0% | |
| TTGGAGCTTGAGCGCAATGG+TGG | + | chr7.3:82147322-82147341 | MS.gene54342:CDS | 55.0% | |
| GCGTTGGAGCTTGAGCGCAA+TGG | + | chr7.3:82147319-82147338 | MS.gene54342:CDS | 60.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr7.3 | gene | 82147271 | 82147637 | 82147271 | ID=MS.gene54342 |
| chr7.3 | mRNA | 82147271 | 82147637 | 82147271 | ID=MS.gene54342.t1;Parent=MS.gene54342 |
| chr7.3 | exon | 82147271 | 82147383 | 82147271 | ID=MS.gene54342.t1.exon1;Parent=MS.gene54342.t1 |
| chr7.3 | CDS | 82147271 | 82147383 | 82147271 | ID=cds.MS.gene54342.t1;Parent=MS.gene54342.t1 |
| chr7.3 | exon | 82147556 | 82147637 | 82147556 | ID=MS.gene54342.t1.exon2;Parent=MS.gene54342.t1 |
| chr7.3 | CDS | 82147556 | 82147637 | 82147556 | ID=cds.MS.gene54342.t1;Parent=MS.gene54342.t1 |
| Gene Sequence |
| Protein sequence |
