AlfalfaGEDB | MS.gene54690

M. sativa cultivar XinJiangDaYe / MS.gene54690

KEGG: K17893
GO: GO:0003674 GO:0003824 GO:0008150 GO:0008152 GO:0009916 GO:0016491 GO:0016679 GO:0016682 GO:0055114
PFAM: PF01786.20

Nr:

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene54690.t1	AGQ42774.1	97.7	175	4	0	1	175	161	335	2.60E-93	351.3

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Swissprot:

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene54690.t1	Q07185	94.3	175	10	0	1	175	147	321	5.5e-92	338.2

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Trembl:

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene54690.t1	S5FNL5	97.7	175	4	0	1	175	161	335	1.9e-93	351.3

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TFs/TRs:

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Gene ID	Type	Classification
No data available in table

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Protein Kinases:

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Gene ID	Type	Classification
No data available in table

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Network:

Co-expression Network:

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Gene1	Gene2	correlation coefficient	p_value	FDR
MS.gene054124	MS.gene54690	0.82016	7.94E-53	-1.69E-46
MS.gene057598	MS.gene54690	0.822123	2.80E-53	-1.69E-46

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PPI:

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Gene1	Gene2	Type
No data available in table

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Query id	Subject id	identity %	alignment length	mismatches	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene54690.t1	MTR_5g026620	97.143	175	5	1	175	88	262	9.88e-126	354
MS.gene54690.t1	MTR_5g026620	97.143	175	5	1	175	156	330	1.28e-124	354
MS.gene54690.t1	MTR_4g094222	76.571	175	41	1	175	171	345	6.14e-101	294
MS.gene54690.t1	MTR_5g070680	73.143	175	47	1	175	149	323	6.34e-96	281
MS.gene54690.t1	MTR_5g070870	73.143	175	47	1	175	150	324	1.50e-89	265

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Query id	Subject id	identity %	alignment length	mismatches	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene54690.t1	AT3G22370	92.571	175	13	1	175	180	354	6.76e-119	340
MS.gene54690.t1	AT3G22360	88.571	175	20	1	175	151	325	3.62e-114	327
MS.gene54690.t1	AT3G27620	85.714	175	25	1	175	155	329	7.36e-110	316
MS.gene54690.t1	AT1G32350	77.143	175	40	1	175	144	318	2.25e-102	297
MS.gene54690.t1	AT5G64210	78.286	175	38	1	175	179	353	3.43e-102	298
MS.gene54690.t1	AT1G32350	78.205	156	34	1	156	144	299	2.79e-91	268

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Find 47 sgRNAs with CRISPR-Local

Find 66 sgRNAs with CRISPR-GE

CRISPR-Local

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CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
AAATTTGCACACCGTATGGT+TGG	0.563076	6.1:-3805293	MS.gene54690:CDS
AACCATACGGTGTGCAAATT+TGG	0.112313	6.1:+3805295	None:intergenic
AAGCATTGAAGAAAACACCC+TGG	0.672042	6.1:+3805342	None:intergenic
AATCGGCGCAATGATTTGCA+GGG	0.601582	6.1:+3805488	None:intergenic
ACCATACGGTGTGCAAATTT+GGG	0.381391	6.1:+3805296	None:intergenic
ACCCAAATTTGCACACCGTA+TGG	0.511597	6.1:-3805297	MS.gene54690:CDS
AGCACTCCTCCTACCATGCC+AGG	0.475186	6.1:+3805515	None:intergenic
AGGGAGGCTGCTGCTCCAAT+TGG	0.436968	6.1:-3804629	MS.gene54690:CDS
AGTAGCAGCTGTCCCTGGCA+TGG	0.607794	6.1:-3805528	MS.gene54690:CDS
AGTGAATAGCTTCTTCTTCA+AGG	0.415906	6.1:+3805267	None:intergenic

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CRISPR-GE

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sgRNA_sequence	Strand	Position	Region	GC_content
AAAGAAACTAATGGTGTGTT+TGG	-	chr6.1:3805206-3805225	MS.gene54690:CDS	30.0%
CACTCCGAATCATAATAAAT+TGG	+	chr6.1:3805335-3805354	None:intergenic	30.0%
TTCATGTGAGTATGTTGAAT+GGG	-	chr6.1:3805447-3805466	MS.gene54690:CDS	30.0%
AGTGAATAGCTTCTTCTTCA+AGG	+	chr6.1:3804897-3804916	None:intergenic	35.0%
CAATAGTCAATAGCAATAGC+TGG	+	chr6.1:3804976-3804995	None:intergenic	35.0%
CATTCAACATACTCACATGA+AGG	+	chr6.1:3805448-3805467	None:intergenic	35.0%
CTTCATGTGAGTATGTTGAA+TGG	-	chr6.1:3805446-3805465	MS.gene54690:CDS	35.0%
TCCCCAAAACTCTACTTTAA+GGG	-	chr6.1:3805002-3805021	MS.gene54690:intron	35.0%
TTCCCCAAAACTCTACTTTA+AGG	-	chr6.1:3805001-3805020	MS.gene54690:intron	35.0%
TTGCAGGATATACATTACCA+AGG	-	chr6.1:3805499-3805518	MS.gene54690:CDS	35.0%

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Chromosome	Type	Strat	End	Strand	Name
chr6.1	gene	3804619	3805564	3804619	ID=MS.gene54690
chr6.1	mRNA	3804619	3805564	3804619	ID=MS.gene54690.t1;Parent=MS.gene54690
chr6.1	exon	3805097	3805564	3805097	ID=MS.gene54690.t1.exon1;Parent=MS.gene54690.t1
chr6.1	CDS	3805097	3805564	3805097	ID=cds.MS.gene54690.t1;Parent=MS.gene54690.t1
chr6.1	exon	3804619	3804678	3804619	ID=MS.gene54690.t1.exon2;Parent=MS.gene54690.t1
chr6.1	CDS	3804619	3804678	3804619	ID=cds.MS.gene54690.t1;Parent=MS.gene54690.t1

Gene Sequence

>MS.gene54690

ATGATGCTTGAGACAGTAGCAGCTGTCCCTGGCATGGTAGGAGGAGTGCTGCTTCCCTGCAAATCATTGCGCCGATTCGAACACAGTGGTGGATGGATCAAAGCTTTGTTAGAAGAAGCAGAGAACGAGCGCATGCACCTAATGACTTTCATGGAAGTGGCAAAACCAAAGTGGTATGAACGTGCACTTGTCATAACTGTCCAGGGTGTTTTCTTCAATGCTTATTTCTTAGGCTATTTGCTCTCACCCAAATTTGCACACCGTATGGTTGGTTACCTTGAAGAAGAAGCTATTCACTCATACACTGAGTTTCTCAAGGAGCTTGACAAGGGTAATATTGAAAATGTCCCTGTTCCAGCTATTGCTATTGACTATTGGCAGCTTCCCCAAAACTCTACTTTAAGGGATGTTGTAGAGGTTGTCAGAGCTGATGAAGCACATCATCGCGATGTTGACCACTTTGCATCGGTATGTTGTCTATCCCTTTGTCTCGCTCTCACTCAATCTATCTATTTATTTATCTATACATCAATAATAATAATAAATTATCAAGTAAACATGTGTAAGGTTATCCTCTCAGTTTTTCAAAAGAAACTAATGGTGTGTTTGGTTTTATGTCAACCATGCCAATCTCGTGTGCTAAGGCAAGATTTACTGTGAATTTGTGAAGCTACAAATTGATCTGTCTTTTTATTTTTCCAAACATAATGTACCCAATTTATTATGATTCGGAGTGTATACACATTTCTCAAACTGTGACATAGATAAGTCTTTATACATTCAAAATCATAGTTGAGTCTTTTTAGAAATGGAAAAAATCTTAGATCCTTCATGTGAGTATGTTGAATGGGATTCAAAAGTTGTGTTTTCATGTAATGAATTGCAGGATATACATTACCAAGGACGAGAGTTAAGGGAGGCTGCTGCTCCAATTGGTTATCACTAG

Protein sequence

>MS.gene54690.t1

MMLETVAAVPGMVGGVLLPCKSLRRFEHSGGWIKALLEEAENERMHLMTFMEVAKPKWYERALVITVQGVFFNAYFLGYLLSPKFAHRMVGYLEEEAIHSYTEFLKELDKGNIENVPVPAIAIDYWQLPQNSTLRDVVEVVRADEAHHRDVDHFASDIHYQGRELREAAAPIGYH