Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene54690.t1 | AGQ42774.1 | 97.7 | 175 | 4 | 0 | 1 | 175 | 161 | 335 | 2.60E-93 | 351.3 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene54690.t1 | Q07185 | 94.3 | 175 | 10 | 0 | 1 | 175 | 147 | 321 | 5.5e-92 | 338.2 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene54690.t1 | S5FNL5 | 97.7 | 175 | 4 | 0 | 1 | 175 | 161 | 335 | 1.9e-93 | 351.3 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene054124 | MS.gene54690 | 0.82016 | 7.94E-53 | -1.69E-46 |
| MS.gene057598 | MS.gene54690 | 0.822123 | 2.80E-53 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene54690.t1 | MTR_5g026620 | 97.143 | 175 | 5 | 0 | 1 | 175 | 88 | 262 | 9.88e-126 | 354 |
| MS.gene54690.t1 | MTR_5g026620 | 97.143 | 175 | 5 | 0 | 1 | 175 | 156 | 330 | 1.28e-124 | 354 |
| MS.gene54690.t1 | MTR_4g094222 | 76.571 | 175 | 41 | 0 | 1 | 175 | 171 | 345 | 6.14e-101 | 294 |
| MS.gene54690.t1 | MTR_5g070680 | 73.143 | 175 | 47 | 0 | 1 | 175 | 149 | 323 | 6.34e-96 | 281 |
| MS.gene54690.t1 | MTR_5g070870 | 73.143 | 175 | 47 | 0 | 1 | 175 | 150 | 324 | 1.50e-89 | 265 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene54690.t1 | AT3G22370 | 92.571 | 175 | 13 | 0 | 1 | 175 | 180 | 354 | 6.76e-119 | 340 |
| MS.gene54690.t1 | AT3G22360 | 88.571 | 175 | 20 | 0 | 1 | 175 | 151 | 325 | 3.62e-114 | 327 |
| MS.gene54690.t1 | AT3G27620 | 85.714 | 175 | 25 | 0 | 1 | 175 | 155 | 329 | 7.36e-110 | 316 |
| MS.gene54690.t1 | AT1G32350 | 77.143 | 175 | 40 | 0 | 1 | 175 | 144 | 318 | 2.25e-102 | 297 |
| MS.gene54690.t1 | AT5G64210 | 78.286 | 175 | 38 | 0 | 1 | 175 | 179 | 353 | 3.43e-102 | 298 |
| MS.gene54690.t1 | AT1G32350 | 78.205 | 156 | 34 | 0 | 1 | 156 | 144 | 299 | 2.79e-91 | 268 |
Find 47 sgRNAs with CRISPR-Local
Find 66 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| AACCATACGGTGTGCAAATT+TGG | 0.112313 | 6.1:+3805295 | None:intergenic |
| TTCCCCAAAACTCTACTTTA+AGG | 0.165997 | 6.1:-3805160 | MS.gene54690:CDS |
| TTCTTCAATGCTTATTTCTT+AGG | 0.252741 | 6.1:-3805332 | MS.gene54690:CDS |
| CATCCCTTAAAGTAGAGTTT+TGG | 0.284733 | 6.1:+3805156 | None:intergenic |
| CCACTTCCATGAAAGTCATT+AGG | 0.338652 | 6.1:+3805405 | None:intergenic |
| CATGCACCTAATGACTTTCA+TGG | 0.370912 | 6.1:-3805411 | MS.gene54690:CDS |
| CAGCTATTGCTATTGACTAT+TGG | 0.379901 | 6.1:-3805187 | MS.gene54690:CDS |
| ACCATACGGTGTGCAAATTT+GGG | 0.381391 | 6.1:+3805296 | None:intergenic |
| GAATCGGCGCAATGATTTGC+AGG | 0.383270 | 6.1:+3805487 | None:intergenic |
| TCCCCAAAACTCTACTTTAA+GGG | 0.386600 | 6.1:-3805159 | MS.gene54690:CDS |
| TCAATAGCAATAGCTGGAAC+AGG | 0.408846 | 6.1:+3805194 | None:intergenic |
| AGTGAATAGCTTCTTCTTCA+AGG | 0.415906 | 6.1:+3805267 | None:intergenic |
| AGGGAGGCTGCTGCTCCAAT+TGG | 0.436968 | 6.1:-3804629 | MS.gene54690:CDS |
| ATTACCAAGGACGAGAGTTA+AGG | 0.452922 | 6.1:-3804649 | MS.gene54690:CDS |
| TTACCAAGGACGAGAGTTAA+GGG | 0.457110 | 6.1:-3804648 | MS.gene54690:CDS |
| AGCACTCCTCCTACCATGCC+AGG | 0.475186 | 6.1:+3805515 | None:intergenic |
| TGCACTTGTCATAACTGTCC+AGG | 0.484144 | 6.1:-3805360 | MS.gene54690:CDS |
| GGTCTCTAGTGATAACCAAT+TGG | 0.490464 | 6.1:+3804614 | None:intergenic |
| TACTTTAAGGGATGTTGTAG+AGG | 0.505163 | 6.1:-3805147 | MS.gene54690:CDS |
| ACCCAAATTTGCACACCGTA+TGG | 0.511597 | 6.1:-3805297 | MS.gene54690:CDS |
| CCTAATGACTTTCATGGAAG+TGG | 0.513462 | 6.1:-3805405 | MS.gene54690:CDS |
| GAGACAGTAGCAGCTGTCCC+TGG | 0.518698 | 6.1:-3805533 | MS.gene54690:CDS |
| ATCCACCACTGTGTTCGAAT+CGG | 0.528479 | 6.1:+3805471 | None:intergenic |
| CCTCCCTTAACTCTCGTCCT+TGG | 0.532033 | 6.1:+3804645 | None:intergenic |
| GCAGCTGTCCCTGGCATGGT+AGG | 0.538784 | 6.1:-3805524 | MS.gene54690:CDS |
| AGTGCACGTTCATACCACTT+TGG | 0.558067 | 6.1:+3805377 | None:intergenic |
| GCTGTCCCTGGCATGGTAGG+AGG | 0.558372 | 6.1:-3805521 | MS.gene54690:CDS |
| CAATAGTCAATAGCAATAGC+TGG | 0.560152 | 6.1:+3805188 | None:intergenic |
| AAATTTGCACACCGTATGGT+TGG | 0.563076 | 6.1:-3805293 | MS.gene54690:CDS |
| GTTTCTCAAGGAGCTTGACA+AGG | 0.563279 | 6.1:-3805234 | MS.gene54690:CDS |
| CTCATACACTGAGTTTCTCA+AGG | 0.566793 | 6.1:-3805246 | MS.gene54690:CDS |
| GATTCGAACACAGTGGTGGA+TGG | 0.577196 | 6.1:-3805469 | MS.gene54690:CDS |
| CGCCGATTCGAACACAGTGG+TGG | 0.599487 | 6.1:-3805473 | MS.gene54690:CDS |
| AATCGGCGCAATGATTTGCA+GGG | 0.601582 | 6.1:+3805488 | None:intergenic |
| AGTAGCAGCTGTCCCTGGCA+TGG | 0.607794 | 6.1:-3805528 | MS.gene54690:CDS |
| CAATAGCAATAGCTGGAACA+GGG | 0.618110 | 6.1:+3805195 | None:intergenic |
| CTTCAAGGTAACCAACCATA+CGG | 0.621895 | 6.1:+3805282 | None:intergenic |
| GCACTCCTCCTACCATGCCA+GGG | 0.622607 | 6.1:+3805516 | None:intergenic |
| CGATGTTGACCACTTTGCAT+CGG | 0.633288 | 6.1:-3805096 | MS.gene54690:intron |
| GCACTTGTCATAACTGTCCA+GGG | 0.642158 | 6.1:-3805359 | MS.gene54690:CDS |
| GACAACATACCGATGCAAAG+TGG | 0.643388 | 6.1:+3805087 | None:intergenic |
| TTTCTCAAGGAGCTTGACAA+GGG | 0.653346 | 6.1:-3805233 | MS.gene54690:CDS |
| TGGAAGTGGCAAAACCAAAG+TGG | 0.670752 | 6.1:-3805391 | MS.gene54690:CDS |
| AAGCATTGAAGAAAACACCC+TGG | 0.672042 | 6.1:+3805342 | None:intergenic |
| TTGCAGGATATACATTACCA+AGG | 0.701646 | 6.1:-3804662 | MS.gene54690:intron |
| CCAAGGACGAGAGTTAAGGG+AGG | 0.747025 | 6.1:-3804645 | MS.gene54690:CDS |
| TTGCGCCGATTCGAACACAG+TGG | 0.778464 | 6.1:-3805476 | MS.gene54690:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !!! | AGTTTTTCAAAAGAAACTAA+TGG | - | chr6.1:3805197-3805216 | MS.gene54690:CDS | 20.0% |
| ! | ACTCCGAATCATAATAAATT+GGG | + | chr6.1:3805334-3805353 | None:intergenic | 25.0% |
| ! | GTACCCAATTTATTATGATT+CGG | - | chr6.1:3805328-3805347 | MS.gene54690:CDS | 25.0% |
| ! | TAAATTGGGTACATTATGTT+TGG | + | chr6.1:3805320-3805339 | None:intergenic | 25.0% |
| ! | TTATCAAGTAAACATGTGTA+AGG | - | chr6.1:3805164-3805183 | MS.gene54690:CDS | 25.0% |
| !! | TTCTTCAATGCTTATTTCTT+AGG | - | chr6.1:3804829-3804848 | MS.gene54690:intron | 25.0% |
| !!! | TAGTTGAGTCTTTTTAGAAA+TGG | - | chr6.1:3805408-3805427 | MS.gene54690:CDS | 25.0% |
| AAAGAAACTAATGGTGTGTT+TGG | - | chr6.1:3805206-3805225 | MS.gene54690:CDS | 30.0% | |
| CACTCCGAATCATAATAAAT+TGG | + | chr6.1:3805335-3805354 | None:intergenic | 30.0% | |
| TTCATGTGAGTATGTTGAAT+GGG | - | chr6.1:3805447-3805466 | MS.gene54690:CDS | 30.0% | |
| ! | ATCCCTTAAAGTAGAGTTTT+GGG | + | chr6.1:3805007-3805026 | None:intergenic | 30.0% |
| ! | GTTTTCATGTAATGAATTGC+AGG | - | chr6.1:3805483-3805502 | MS.gene54690:CDS | 30.0% |
| !! | GTTTCTTTTGAAAAACTGAG+AGG | + | chr6.1:3805194-3805213 | None:intergenic | 30.0% |
| AGTGAATAGCTTCTTCTTCA+AGG | + | chr6.1:3804897-3804916 | None:intergenic | 35.0% | |
| CAATAGTCAATAGCAATAGC+TGG | + | chr6.1:3804976-3804995 | None:intergenic | 35.0% | |
| CATTCAACATACTCACATGA+AGG | + | chr6.1:3805448-3805467 | None:intergenic | 35.0% | |
| CTTCATGTGAGTATGTTGAA+TGG | - | chr6.1:3805446-3805465 | MS.gene54690:CDS | 35.0% | |
| TCCCCAAAACTCTACTTTAA+GGG | - | chr6.1:3805002-3805021 | MS.gene54690:intron | 35.0% | |
| TTCCCCAAAACTCTACTTTA+AGG | - | chr6.1:3805001-3805020 | MS.gene54690:intron | 35.0% | |
| TTGCAGGATATACATTACCA+AGG | - | chr6.1:3805499-3805518 | MS.gene54690:CDS | 35.0% | |
| ! | CAGCTATTGCTATTGACTAT+TGG | - | chr6.1:3804974-3804993 | MS.gene54690:intron | 35.0% |
| ! | CATCCCTTAAAGTAGAGTTT+TGG | + | chr6.1:3805008-3805027 | None:intergenic | 35.0% |
| ! | TACTTTAAGGGATGTTGTAG+AGG | - | chr6.1:3805014-3805033 | MS.gene54690:intron | 35.0% |
| ! | TCCCTTAAAGTAGAGTTTTG+GGG | + | chr6.1:3805006-3805025 | None:intergenic | 35.0% |
| AAATTTGCACACCGTATGGT+TGG | - | chr6.1:3804868-3804887 | MS.gene54690:intron | 40.0% | |
| AACCATACGGTGTGCAAATT+TGG | + | chr6.1:3804869-3804888 | None:intergenic | 40.0% | |
| ACCATACGGTGTGCAAATTT+GGG | + | chr6.1:3804868-3804887 | None:intergenic | 40.0% | |
| ATTACCAAGGACGAGAGTTA+AGG | - | chr6.1:3805512-3805531 | MS.gene54690:CDS | 40.0% | |
| CAATAGCAATAGCTGGAACA+GGG | + | chr6.1:3804969-3804988 | None:intergenic | 40.0% | |
| CATGCACCTAATGACTTTCA+TGG | - | chr6.1:3804750-3804769 | MS.gene54690:intron | 40.0% | |
| CCACTTCCATGAAAGTCATT+AGG | + | chr6.1:3804759-3804778 | None:intergenic | 40.0% | |
| CCTAATGACTTTCATGGAAG+TGG | - | chr6.1:3804756-3804775 | MS.gene54690:intron | 40.0% | |
| CTCATACACTGAGTTTCTCA+AGG | - | chr6.1:3804915-3804934 | MS.gene54690:intron | 40.0% | |
| CTTCAAGGTAACCAACCATA+CGG | + | chr6.1:3804882-3804901 | None:intergenic | 40.0% | |
| TCAATAGCAATAGCTGGAAC+AGG | + | chr6.1:3804970-3804989 | None:intergenic | 40.0% | |
| TTACCAAGGACGAGAGTTAA+GGG | - | chr6.1:3805513-3805532 | MS.gene54690:CDS | 40.0% | |
| TTTCTCAAGGAGCTTGACAA+GGG | - | chr6.1:3804928-3804947 | MS.gene54690:intron | 40.0% | |
| !! | AAGCATTGAAGAAAACACCC+TGG | + | chr6.1:3804822-3804841 | None:intergenic | 40.0% |
| ACCCAAATTTGCACACCGTA+TGG | - | chr6.1:3804864-3804883 | MS.gene54690:intron | 45.0% | |
| AGTGCACGTTCATACCACTT+TGG | + | chr6.1:3804787-3804806 | None:intergenic | 45.0% | |
| ATCCACCACTGTGTTCGAAT+CGG | + | chr6.1:3804693-3804712 | None:intergenic | 45.0% | |
| ATTGAGTGAGAGCGAGACAA+AGG | + | chr6.1:3805104-3805123 | None:intergenic | 45.0% | |
| CGATGTTGACCACTTTGCAT+CGG | - | chr6.1:3805065-3805084 | MS.gene54690:intron | 45.0% | |
| GACAACATACCGATGCAAAG+TGG | + | chr6.1:3805077-3805096 | None:intergenic | 45.0% | |
| GCACTTGTCATAACTGTCCA+GGG | - | chr6.1:3804802-3804821 | MS.gene54690:intron | 45.0% | |
| GTTTCTCAAGGAGCTTGACA+AGG | - | chr6.1:3804927-3804946 | MS.gene54690:intron | 45.0% | |
| TGCACTTGTCATAACTGTCC+AGG | - | chr6.1:3804801-3804820 | MS.gene54690:intron | 45.0% | |
| TGGAAGTGGCAAAACCAAAG+TGG | - | chr6.1:3804770-3804789 | MS.gene54690:intron | 45.0% | |
| TTGAGTGAGAGCGAGACAAA+GGG | + | chr6.1:3805103-3805122 | None:intergenic | 45.0% | |
| ! | AATCGGCGCAATGATTTGCA+GGG | + | chr6.1:3804676-3804695 | None:intergenic | 45.0% |
| CATGCCAATCTCGTGTGCTA+AGG | - | chr6.1:3805241-3805260 | MS.gene54690:CDS | 50.0% | |
| CTTAGCACACGAGATTGGCA+TGG | + | chr6.1:3805243-3805262 | None:intergenic | 50.0% | |
| CTTGCCTTAGCACACGAGAT+TGG | + | chr6.1:3805248-3805267 | None:intergenic | 50.0% | |
| ! | GAATCGGCGCAATGATTTGC+AGG | + | chr6.1:3804677-3804696 | None:intergenic | 50.0% |
| ! | GATTCGAACACAGTGGTGGA+TGG | - | chr6.1:3804692-3804711 | MS.gene54690:intron | 50.0% |
| CCAAGGACGAGAGTTAAGGG+AGG | - | chr6.1:3805516-3805535 | MS.gene54690:CDS | 55.0% | |
| CCTCCCTTAACTCTCGTCCT+TGG | + | chr6.1:3805519-3805538 | None:intergenic | 55.0% | |
| !! | TTGCGCCGATTCGAACACAG+TGG | - | chr6.1:3804685-3804704 | MS.gene54690:intron | 55.0% |
| AGCACTCCTCCTACCATGCC+AGG | + | chr6.1:3804649-3804668 | None:intergenic | 60.0% | |
| AGGGAGGCTGCTGCTCCAAT+TGG | - | chr6.1:3805532-3805551 | MS.gene54690:CDS | 60.0% | |
| AGTAGCAGCTGTCCCTGGCA+TGG | - | chr6.1:3804633-3804652 | MS.gene54690:CDS | 60.0% | |
| GAGACAGTAGCAGCTGTCCC+TGG | - | chr6.1:3804628-3804647 | MS.gene54690:CDS | 60.0% | |
| GCACTCCTCCTACCATGCCA+GGG | + | chr6.1:3804648-3804667 | None:intergenic | 60.0% | |
| !! | CGCCGATTCGAACACAGTGG+TGG | - | chr6.1:3804688-3804707 | MS.gene54690:intron | 60.0% |
| GCAGCTGTCCCTGGCATGGT+AGG | - | chr6.1:3804637-3804656 | MS.gene54690:CDS | 65.0% | |
| GCTGTCCCTGGCATGGTAGG+AGG | - | chr6.1:3804640-3804659 | MS.gene54690:CDS | 65.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr6.1 | gene | 3804619 | 3805564 | 3804619 | ID=MS.gene54690 |
| chr6.1 | mRNA | 3804619 | 3805564 | 3804619 | ID=MS.gene54690.t1;Parent=MS.gene54690 |
| chr6.1 | exon | 3805097 | 3805564 | 3805097 | ID=MS.gene54690.t1.exon1;Parent=MS.gene54690.t1 |
| chr6.1 | CDS | 3805097 | 3805564 | 3805097 | ID=cds.MS.gene54690.t1;Parent=MS.gene54690.t1 |
| chr6.1 | exon | 3804619 | 3804678 | 3804619 | ID=MS.gene54690.t1.exon2;Parent=MS.gene54690.t1 |
| chr6.1 | CDS | 3804619 | 3804678 | 3804619 | ID=cds.MS.gene54690.t1;Parent=MS.gene54690.t1 |
| Gene Sequence |
| Protein sequence |
