Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene54815.t1 | XP_013468406.1 | 98.5 | 263 | 4 | 0 | 2 | 264 | 245 | 507 | 2.80E-155 | 557.8 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene54815.t1 | Q9FND4 | 73.4 | 263 | 68 | 1 | 2 | 262 | 253 | 515 | 2.5e-120 | 433.0 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene54815.t1 | A0A072VM11 | 98.5 | 263 | 4 | 0 | 2 | 264 | 245 | 507 | 2.0e-155 | 557.8 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene059032 | MS.gene54815 | 0.816822 | 4.54E-52 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene54815.t1 | MTR_1g069345 | 98.479 | 263 | 4 | 0 | 2 | 264 | 245 | 507 | 0.0 | 546 |
| MS.gene54815.t1 | MTR_5g090420 | 50.794 | 252 | 122 | 1 | 10 | 261 | 300 | 549 | 2.28e-87 | 271 |
| MS.gene54815.t1 | MTR_3g449990 | 48.846 | 260 | 131 | 1 | 2 | 261 | 299 | 556 | 4.37e-87 | 270 |
| MS.gene54815.t1 | MTR_1g072140 | 28.302 | 265 | 181 | 7 | 1 | 260 | 52 | 312 | 9.27e-29 | 111 |
| MS.gene54815.t1 | MTR_4g119620 | 30.159 | 252 | 163 | 9 | 16 | 260 | 76 | 321 | 9.64e-26 | 103 |
| MS.gene54815.t1 | MTR_8g092620 | 26.506 | 249 | 176 | 6 | 13 | 260 | 55 | 297 | 5.49e-17 | 79.3 |
| MS.gene54815.t1 | MTR_8g092620 | 25.911 | 247 | 176 | 6 | 13 | 258 | 62 | 302 | 3.80e-15 | 74.3 |
| MS.gene54815.t1 | MTR_4g105700 | 25.000 | 288 | 174 | 7 | 13 | 260 | 190 | 475 | 1.94e-14 | 73.2 |
| MS.gene54815.t1 | MTR_7g086360 | 24.904 | 261 | 169 | 9 | 14 | 259 | 94 | 342 | 7.94e-13 | 67.8 |
| MS.gene54815.t1 | MTR_1g097140 | 24.739 | 287 | 172 | 10 | 14 | 260 | 119 | 401 | 1.47e-12 | 67.4 |
| MS.gene54815.t1 | MTR_4g102520 | 24.219 | 256 | 182 | 8 | 13 | 260 | 333 | 584 | 3.57e-12 | 66.2 |
| MS.gene54815.t1 | MTR_5g022690 | 22.182 | 275 | 193 | 6 | 2 | 260 | 164 | 433 | 7.86e-11 | 62.4 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene54815.t1 | AT5G43920 | 73.384 | 263 | 68 | 1 | 2 | 262 | 253 | 515 | 7.27e-147 | 421 |
| MS.gene54815.t1 | AT5G08560 | 48.077 | 260 | 133 | 1 | 2 | 261 | 303 | 560 | 1.88e-84 | 263 |
| MS.gene54815.t1 | AT5G08560 | 48.077 | 260 | 133 | 1 | 2 | 261 | 303 | 560 | 1.88e-84 | 263 |
| MS.gene54815.t1 | AT5G08560 | 48.077 | 260 | 133 | 1 | 2 | 261 | 303 | 560 | 1.88e-84 | 263 |
| MS.gene54815.t1 | AT3G49660 | 32.016 | 253 | 159 | 10 | 14 | 260 | 67 | 312 | 3.14e-27 | 107 |
| MS.gene54815.t1 | AT4G02730 | 29.615 | 260 | 169 | 10 | 9 | 260 | 76 | 329 | 1.76e-19 | 86.7 |
| MS.gene54815.t1 | AT5G64730 | 26.984 | 252 | 177 | 6 | 10 | 260 | 52 | 297 | 1.58e-17 | 80.9 |
| MS.gene54815.t1 | AT3G27640 | 23.529 | 289 | 174 | 10 | 16 | 261 | 107 | 391 | 2.77e-14 | 72.8 |
| MS.gene54815.t1 | AT3G27640 | 23.529 | 289 | 174 | 10 | 16 | 261 | 126 | 410 | 2.91e-14 | 72.8 |
| MS.gene54815.t1 | AT1G10580 | 25.098 | 255 | 181 | 6 | 13 | 260 | 321 | 572 | 1.15e-13 | 70.9 |
| MS.gene54815.t1 | AT2G43770 | 23.735 | 257 | 177 | 9 | 14 | 259 | 92 | 340 | 6.32e-13 | 68.2 |
| MS.gene54815.t1 | AT2G41500 | 25.000 | 248 | 176 | 6 | 16 | 260 | 253 | 493 | 1.01e-12 | 68.2 |
| MS.gene54815.t1 | AT2G41500 | 25.000 | 248 | 176 | 6 | 16 | 260 | 253 | 493 | 1.01e-12 | 68.2 |
Find 0 sgRNAs with CRISPR-Local
Find 102 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | TATTATTGGATAAAGAAATT+CGG | - | 10889:10899-10918 | MS.gene54815:CDS | 15.0% |
| !! | TTAATTAATACACTTAAGAA+TGG | + | 10889:11184-11203 | None:intergenic | 15.0% |
| !!! | ATATATGTTTTGATATCTTT+CGG | - | 10889:10252-10271 | MS.gene54815:CDS | 15.0% |
| ! | ATGATCATACAATTCGTATA+TGG | - | 10889:11403-11422 | MS.gene54815:intron | 25.0% |
| ! | GTATCTAATCAGCATATTAT+TGG | - | 10889:10885-10904 | MS.gene54815:CDS | 25.0% |
| ! | TGATCATACAATTCGTATAT+GGG | - | 10889:11404-11423 | MS.gene54815:intron | 25.0% |
| !! | AGAAATTCGGATTTTACATT+TGG | - | 10889:10912-10931 | MS.gene54815:CDS | 25.0% |
| AACAGTAAGATCTACAACTT+TGG | + | 10889:10851-10870 | None:intergenic | 30.0% | |
| AACAGTGCAAATATGTGATT+AGG | - | 10889:11094-11113 | MS.gene54815:intron | 30.0% | |
| ACAGTAAGATCTACAACTTT+GGG | + | 10889:10850-10869 | None:intergenic | 30.0% | |
| CAAATGTGGTAAATTGGTAA+TGG | - | 10889:10469-10488 | MS.gene54815:CDS | 30.0% | |
| CATGAGTATTTCAGAACATT+AGG | + | 10889:10514-10533 | None:intergenic | 30.0% | |
| GATCATACAATTCGTATATG+GGG | - | 10889:11405-11424 | MS.gene54815:intron | 30.0% | |
| TCAAACTATCAAACATCTTG+TGG | + | 10889:10284-10303 | None:intergenic | 30.0% | |
| ! | ACCTGCATGACATAAATTTT+GGG | + | 10889:11267-11286 | None:intergenic | 30.0% |
| ! | TACCTGCATGACATAAATTT+TGG | + | 10889:11268-11287 | None:intergenic | 30.0% |
| !!! | CACTACAAGAAGGTTTTTTT+TGG | + | 10889:10205-10224 | None:intergenic | 30.0% |
| AAACCGAAATGGATGTGATA+GGG | + | 10889:10972-10991 | None:intergenic | 35.0% | |
| AACTCAACACAATTTGTGTG+CGG | - | 10889:10745-10764 | MS.gene54815:CDS | 35.0% | |
| AACTCACACAATTTACAGTC+AGG | + | 10889:11344-11363 | None:intergenic | 35.0% | |
| AAGCATACATTTGGGAATGA+TGG | - | 10889:10691-10710 | MS.gene54815:CDS | 35.0% | |
| ACCACTAGATTGAGATAGTT+TGG | + | 10889:10376-10395 | None:intergenic | 35.0% | |
| ACTCACACAATTTACAGTCA+GGG | + | 10889:11343-11362 | None:intergenic | 35.0% | |
| CAAGCTCAAATGTGGTAAAT+TGG | - | 10889:10463-10482 | MS.gene54815:CDS | 35.0% | |
| CAATTTACCACATTTGAGCT+TGG | + | 10889:10465-10484 | None:intergenic | 35.0% | |
| CACACAAATTGTGTTGAGTT+GGG | + | 10889:10745-10764 | None:intergenic | 35.0% | |
| GATAGTACATTTATTGCCAG+TGG | - | 10889:11135-11154 | MS.gene54815:intron | 35.0% | |
| GCTAACATATCACTACAAGA+AGG | + | 10889:10215-10234 | None:intergenic | 35.0% | |
| GTTTATACAACTTGTCAGAC+AGG | - | 10889:10394-10413 | MS.gene54815:intron | 35.0% | |
| TGACTGTAAATTGTGTGAGT+TGG | - | 10889:11343-11362 | MS.gene54815:intron | 35.0% | |
| TTTACATTTGGCAACCAATG+CGG | - | 10889:10924-10943 | MS.gene54815:CDS | 35.0% | |
| ! | AAAATACAGCGTGTGGATTA+CGG | + | 10889:11224-11243 | None:intergenic | 35.0% |
| ! | AGGTATACATCTGGAATTCT+CGG | - | 10889:11283-11302 | MS.gene54815:intron | 35.0% |
| ! | CCTGCATGACATAAATTTTG+GGG | + | 10889:11266-11285 | None:intergenic | 35.0% |
| ! | GTTTGATAGTTTGACTGTCA+AGG | - | 10889:10290-10309 | MS.gene54815:CDS | 35.0% |
| ! | TATGTGATTAGGTCCTGTTT+TGG | - | 10889:11105-11124 | MS.gene54815:intron | 35.0% |
| !! | ATGCCAATAGAGGTTTTATC+TGG | - | 10889:11312-11331 | MS.gene54815:intron | 35.0% |
| !! | TGCCAATAGAGGTTTTATCT+GGG | - | 10889:11313-11332 | MS.gene54815:intron | 35.0% |
| !! | TGTTCTGAAATACTCATGAC+AGG | - | 10889:10516-10535 | MS.gene54815:CDS | 35.0% |
| ACATTGAAACACACACTCAG+CGG | - | 10889:10559-10578 | MS.gene54815:CDS | 40.0% | |
| ACTATCTGATCCACCAAAAC+AGG | + | 10889:11121-11140 | None:intergenic | 40.0% | |
| ATGGAAACGAACTAAGAGCA+TGG | - | 10889:10809-10828 | MS.gene54815:CDS | 40.0% | |
| ATGTCATGCAGGTATACATC+TGG | - | 10889:11274-11293 | MS.gene54815:intron | 40.0% | |
| CCCCAAAATTTATGTCATGC+AGG | - | 10889:11263-11282 | MS.gene54815:intron | 40.0% | |
| CTTGCAGATGCTAACATTTG+TGG | + | 10889:11381-11400 | None:intergenic | 40.0% | |
| GAAACCGAAATGGATGTGAT+AGG | + | 10889:10973-10992 | None:intergenic | 40.0% | |
| GATAGTTTGACTGTCAAGGA+AGG | - | 10889:10294-10313 | MS.gene54815:CDS | 40.0% | |
| GATCTTACTGTTACTCCAGA+CGG | - | 10889:10859-10878 | MS.gene54815:CDS | 40.0% | |
| GCACACAAATTGTGTTGAGT+TGG | + | 10889:10746-10765 | None:intergenic | 40.0% | |
| GCACATGCAAGCATACATTT+GGG | - | 10889:10683-10702 | MS.gene54815:CDS | 40.0% | |
| GCCAAACTATCTCAATCTAG+TGG | - | 10889:10372-10391 | MS.gene54815:intron | 40.0% | |
| TGACGAAAAAATACAGCGTG+TGG | + | 10889:11231-11250 | None:intergenic | 40.0% | |
| TGCCCAGATAAAACCTCTAT+TGG | + | 10889:11318-11337 | None:intergenic | 40.0% | |
| TTATGCTCCAAGCTCAAATG+TGG | - | 10889:10455-10474 | MS.gene54815:intron | 40.0% | |
| ! | GCTAACATTTGTGGCCTTTT+AGG | + | 10889:11372-11391 | None:intergenic | 40.0% |
| ! | GTGTGAGTTGGAATCCTAAA+AGG | - | 10889:11355-11374 | MS.gene54815:intron | 40.0% |
| ! | TCAACGTGCTGTATCTTTTG+TGG | - | 10889:10585-10604 | MS.gene54815:CDS | 40.0% |
| !!! | ACATGGAAGTTTTGAAGCTG+TGG | - | 10889:10647-10666 | MS.gene54815:CDS | 40.0% |
| !!! | CATGGAAGTTTTGAAGCTGT+GGG | - | 10889:10648-10667 | MS.gene54815:CDS | 40.0% |
| AAGAAGCCACTGAGGTATAC+TGG | - | 10889:11066-11085 | MS.gene54815:CDS | 45.0% | |
| AAGCTGTGGGATGTTGAAAC+TGG | - | 10889:10661-10680 | MS.gene54815:CDS | 45.0% | |
| ACACCAAGTTGCTCACTTGT+GGG | - | 10889:10623-10642 | MS.gene54815:CDS | 45.0% | |
| ACCATCAGCAGAAACCGAAA+TGG | + | 10889:10983-11002 | None:intergenic | 45.0% | |
| ATCTGCATGTGGGATTGTGA+TGG | - | 10889:10790-10809 | MS.gene54815:CDS | 45.0% | |
| CAGTGGCAGTGAAAATTCAG+AGG | - | 10889:11152-11171 | MS.gene54815:intron | 45.0% | |
| CATGACAGGTGCAAGAAGAT+GGG | - | 10889:10530-10549 | MS.gene54815:CDS | 45.0% | |
| CATTGAAACACACACTCAGC+GGG | - | 10889:10560-10579 | MS.gene54815:CDS | 45.0% | |
| CATTGTCAATCTGAACAGCC+AGG | - | 10889:11011-11030 | MS.gene54815:CDS | 45.0% | |
| CTGATTAGATACTCTCCGTC+TGG | + | 10889:10877-10896 | None:intergenic | 45.0% | |
| GCACCCTATCACATCCATTT+CGG | - | 10889:10966-10985 | MS.gene54815:CDS | 45.0% | |
| GGCACATGCAAGCATACATT+TGG | - | 10889:10682-10701 | MS.gene54815:CDS | 45.0% | |
| TCATGACAGGTGCAAGAAGA+TGG | - | 10889:10529-10548 | MS.gene54815:CDS | 45.0% | |
| TCCATTTCGGTTTCTGCTGA+TGG | - | 10889:10979-10998 | MS.gene54815:CDS | 45.0% | |
| TCGGAGTTCAATGCCAATAG+AGG | - | 10889:11302-11321 | MS.gene54815:intron | 45.0% | |
| TGAAATGACTCGTTCCGCAT+TGG | + | 10889:10941-10960 | None:intergenic | 45.0% | |
| TTGTGACCAGTATACCTCAG+TGG | + | 10889:11075-11094 | None:intergenic | 45.0% | |
| ! | AACGAACTAAGAGCATGGAG+AGG | - | 10889:10814-10833 | MS.gene54815:CDS | 45.0% |
| ! | ACATGCAGATGCCCTTTTCA+GGG | + | 10889:10780-10799 | None:intergenic | 45.0% |
| ! | CTCTGAATTTTCACTGCCAC+TGG | + | 10889:11154-11173 | None:intergenic | 45.0% |
| ! | GTGCTGTATCTTTTGTGGCA+TGG | - | 10889:10590-10609 | MS.gene54815:CDS | 45.0% |
| ! | TAAGAGCATGGAGAGGAATG+AGG | - | 10889:10821-10840 | MS.gene54815:CDS | 45.0% |
| !! | GGCAGTTTTGACCCTGAAAA+GGG | - | 10889:10766-10785 | MS.gene54815:CDS | 45.0% |
| !! | GTGATTAGGTCCTGTTTTGG+TGG | - | 10889:11108-11127 | MS.gene54815:intron | 45.0% |
| !! | TGAAAAGGGCATCTGCATGT+GGG | - | 10889:10780-10799 | MS.gene54815:CDS | 45.0% |
| !! | TTGTGTTGAGTTGGGAAACC+AGG | + | 10889:10737-10756 | None:intergenic | 45.0% |
| ACAGCCAGGAGATCCATATG+TGG | - | 10889:11025-11044 | MS.gene54815:CDS | 50.0% | |
| ATCCATATGTGGGACGTAGC+CGG | - | 10889:11036-11055 | MS.gene54815:CDS | 50.0% | |
| CAGCCAGGAGATCCATATGT+GGG | - | 10889:11026-11045 | MS.gene54815:CDS | 50.0% | |
| CAGTGGCTTCTTCCATGTAC+CGG | + | 10889:11058-11077 | None:intergenic | 50.0% | |
| GACACCAAGTTGCTCACTTG+TGG | - | 10889:10622-10641 | MS.gene54815:CDS | 50.0% | |
| GTACATGGAAGAAGCCACTG+AGG | - | 10889:11058-11077 | MS.gene54815:CDS | 50.0% | |
| GTTCCCACAAGTGAGCAACT+TGG | + | 10889:10629-10648 | None:intergenic | 50.0% | |
| GTTGCTCACTTGTGGGAACA+TGG | - | 10889:10630-10649 | MS.gene54815:CDS | 50.0% | |
| TGCGGAACGAGTCATTTCAG+AGG | - | 10889:10942-10961 | MS.gene54815:CDS | 50.0% | |
| TTGTTGTCAGCTCATGTGCC+TGG | - | 10889:10716-10735 | MS.gene54815:CDS | 50.0% | |
| ! | CACATGCAGATGCCCTTTTC+AGG | + | 10889:10781-10800 | None:intergenic | 50.0% |
| !! | CGGCAGTTTTGACCCTGAAA+AGG | - | 10889:10765-10784 | MS.gene54815:CDS | 50.0% |
| !! | CTGAAAAGGGCATCTGCATG+TGG | - | 10889:10779-10798 | MS.gene54815:CDS | 50.0% |
| !! | GTGAGCAACTTGGTGTCATC+AGG | + | 10889:10619-10638 | None:intergenic | 50.0% |
| CGTCCCACATATGGATCTCC+TGG | + | 10889:11032-11051 | None:intergenic | 55.0% | |
| ! | TACCGGCTACGTCCCACATA+TGG | + | 10889:11041-11060 | None:intergenic | 55.0% |
| TGTGGGACGTAGCCGGTACA+TGG | - | 10889:11043-11062 | MS.gene54815:CDS | 60.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| 10889 | gene | 10196 | 11440 | 10196 | ID=MS.gene54815 |
| 10889 | mRNA | 10196 | 11440 | 10196 | ID=MS.gene54815.t1;Parent=MS.gene54815 |
| 10889 | exon | 11438 | 11440 | 11438 | ID=MS.gene54815.t1.exon1;Parent=MS.gene54815.t1 |
| 10889 | CDS | 11438 | 11440 | 11438 | ID=cds.MS.gene54815.t1;Parent=MS.gene54815.t1 |
| 10889 | exon | 10463 | 11098 | 10463 | ID=MS.gene54815.t1.exon2;Parent=MS.gene54815.t1 |
| 10889 | CDS | 10463 | 11098 | 10463 | ID=cds.MS.gene54815.t1;Parent=MS.gene54815.t1 |
| 10889 | exon | 10196 | 10351 | 10196 | ID=MS.gene54815.t1.exon3;Parent=MS.gene54815.t1 |
| 10889 | CDS | 10196 | 10351 | 10196 | ID=cds.MS.gene54815.t1;Parent=MS.gene54815.t1 |
| Gene Sequence |
| Protein sequence |
