Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene55020.t1 | GAU39460.1 | 38 | 108 | 43 | 2 | 1 | 87 | 1 | 105 | 7.00E-10 | 73.2 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene55020.t1 | A0A2Z6NRS6 | 38.0 | 108 | 43 | 2 | 1 | 87 | 1 | 105 | 5.0e-10 | 73.2 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene05548 | MS.gene55020 | 0.808446 | 3.09E-50 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Find 31 sgRNAs with CRISPR-Local
Find 29 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| ATGATCAGTGTAAGGCAATT+TGG | 0.228687 | 7.3:+25206842 | MS.gene55020:CDS |
| GTCTACCAATGGAGAGGATT+TGG | 0.264471 | 7.3:+25206974 | MS.gene55020:CDS |
| TCTACCAATGGAGAGGATTT+GGG | 0.310714 | 7.3:+25206975 | MS.gene55020:CDS |
| GAAGCTTCATTGCTCATATC+TGG | 0.338136 | 7.3:-25207009 | None:intergenic |
| CAGTGGCTCATAGGACCTTC+TGG | 0.346451 | 7.3:-25206799 | None:intergenic |
| TGATCAGTGTAAGGCAATTT+GGG | 0.366956 | 7.3:+25206843 | MS.gene55020:CDS |
| GTACCTTACTTTGGAGTTTG+TGG | 0.381746 | 7.3:+25206951 | MS.gene55020:CDS |
| AACGAGTTAATCACCATTCC+TGG | 0.388834 | 7.3:-25207046 | None:intergenic |
| TCTATCTTGCAGTGGCTCAT+AGG | 0.404363 | 7.3:-25206808 | None:intergenic |
| TACCTTACTTTGGAGTTTGT+GGG | 0.423061 | 7.3:+25206952 | MS.gene55020:CDS |
| TCTTGAGGATGATCAGTGTA+AGG | 0.470969 | 7.3:+25206834 | MS.gene55020:CDS |
| CTTCTACCTCTCGAGTATCC+AGG | 0.495212 | 7.3:+25207028 | MS.gene55020:CDS |
| GGAGCTGAAAGGTGAGACTC+TGG | 0.501297 | 7.3:+25206864 | MS.gene55020:CDS |
| CCTCATTTGTAGTGCGAACA+AGG | 0.509994 | 7.3:-25206897 | None:intergenic |
| GAAGGTAGAGTACCTTACTT+TGG | 0.514894 | 7.3:+25206942 | MS.gene55020:CDS |
| GGAGTTTGTGGGTCTACCAA+TGG | 0.516668 | 7.3:+25206963 | MS.gene55020:CDS |
| GACCCACAAACTCCAAAGTA+AGG | 0.517101 | 7.3:-25206954 | None:intergenic |
| ATTTGTAGTGCGAACAAGGC+CGG | 0.534580 | 7.3:-25206893 | None:intergenic |
| GCTCCCCAAATCCTCTCCAT+TGG | 0.554872 | 7.3:-25206979 | None:intergenic |
| CCTTGTTCGCACTACAAATG+AGG | 0.557277 | 7.3:+25206897 | MS.gene55020:CDS |
| ACCTCTCGAGTATCCAGGAA+TGG | 0.576121 | 7.3:+25207033 | MS.gene55020:CDS |
| CTACCAATGGAGAGGATTTG+GGG | 0.599763 | 7.3:+25206976 | MS.gene55020:CDS |
| CGCACTACAAATGAGGTCGA+TGG | 0.605943 | 7.3:+25206904 | MS.gene55020:CDS |
| CTGCAAGATAGAGTTTCTTG+AGG | 0.626115 | 7.3:+25206819 | MS.gene55020:CDS |
| TTGTGGGTCTACCAATGGAG+AGG | 0.626640 | 7.3:+25206968 | MS.gene55020:CDS |
| TTCCTACAGATGTTGCCAGA+AGG | 0.629071 | 7.3:+25206784 | None:intergenic |
| AAGGCAATTTGGGAGCTGAA+AGG | 0.638835 | 7.3:+25206853 | MS.gene55020:CDS |
| CAAGAAACTCTATCTTGCAG+TGG | 0.642358 | 7.3:-25206816 | None:intergenic |
| ACCATTCCTGGATACTCGAG+AGG | 0.658945 | 7.3:-25207034 | None:intergenic |
| GGTGAGACTCTGGTTTACAC+CGG | 0.688295 | 7.3:+25206874 | MS.gene55020:CDS |
| TGGTTTGCTTGTGAGCACGA+AGG | 0.691002 | 7.3:+25206924 | MS.gene55020:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| ATGATCAGTGTAAGGCAATT+TGG | + | chr7.3:25206842-25206861 | MS.gene55020:CDS | 35.0% | |
| TGATCAGTGTAAGGCAATTT+GGG | + | chr7.3:25206843-25206862 | MS.gene55020:CDS | 35.0% | |
| !! | TACCTTACTTTGGAGTTTGT+GGG | + | chr7.3:25206952-25206971 | MS.gene55020:CDS | 35.0% |
| CAAGAAACTCTATCTTGCAG+TGG | - | chr7.3:25206819-25206838 | None:intergenic | 40.0% | |
| CTGCAAGATAGAGTTTCTTG+AGG | + | chr7.3:25206819-25206838 | MS.gene55020:CDS | 40.0% | |
| GAAGCTTCATTGCTCATATC+TGG | - | chr7.3:25207012-25207031 | None:intergenic | 40.0% | |
| TCTACCAATGGAGAGGATTT+GGG | + | chr7.3:25206975-25206994 | MS.gene55020:CDS | 40.0% | |
| TCTTGAGGATGATCAGTGTA+AGG | + | chr7.3:25206834-25206853 | MS.gene55020:CDS | 40.0% | |
| ! | GAAGGTAGAGTACCTTACTT+TGG | + | chr7.3:25206942-25206961 | MS.gene55020:CDS | 40.0% |
| !! | GTACCTTACTTTGGAGTTTG+TGG | + | chr7.3:25206951-25206970 | MS.gene55020:CDS | 40.0% |
| AAGGCAATTTGGGAGCTGAA+AGG | + | chr7.3:25206853-25206872 | MS.gene55020:CDS | 45.0% | |
| ATTTGTAGTGCGAACAAGGC+CGG | - | chr7.3:25206896-25206915 | None:intergenic | 45.0% | |
| CCTCATTTGTAGTGCGAACA+AGG | - | chr7.3:25206900-25206919 | None:intergenic | 45.0% | |
| CCTTGTTCGCACTACAAATG+AGG | + | chr7.3:25206897-25206916 | MS.gene55020:CDS | 45.0% | |
| CTACCAATGGAGAGGATTTG+GGG | + | chr7.3:25206976-25206995 | MS.gene55020:CDS | 45.0% | |
| GACCCACAAACTCCAAAGTA+AGG | - | chr7.3:25206957-25206976 | None:intergenic | 45.0% | |
| GTCTACCAATGGAGAGGATT+TGG | + | chr7.3:25206974-25206993 | MS.gene55020:CDS | 45.0% | |
| TCTATCTTGCAGTGGCTCAT+AGG | - | chr7.3:25206811-25206830 | None:intergenic | 45.0% | |
| ACCATTCCTGGATACTCGAG+AGG | - | chr7.3:25207037-25207056 | None:intergenic | 50.0% | |
| ACCTCTCGAGTATCCAGGAA+TGG | + | chr7.3:25207033-25207052 | MS.gene55020:CDS | 50.0% | |
| CGCACTACAAATGAGGTCGA+TGG | + | chr7.3:25206904-25206923 | MS.gene55020:CDS | 50.0% | |
| CTTCTACCTCTCGAGTATCC+AGG | + | chr7.3:25207028-25207047 | MS.gene55020:CDS | 50.0% | |
| GGAGTTTGTGGGTCTACCAA+TGG | + | chr7.3:25206963-25206982 | MS.gene55020:CDS | 50.0% | |
| TTGTGGGTCTACCAATGGAG+AGG | + | chr7.3:25206968-25206987 | MS.gene55020:CDS | 50.0% | |
| ! | GGTGAGACTCTGGTTTACAC+CGG | + | chr7.3:25206874-25206893 | MS.gene55020:CDS | 50.0% |
| !! | TGGTTTGCTTGTGAGCACGA+AGG | + | chr7.3:25206924-25206943 | MS.gene55020:CDS | 50.0% |
| CAGTGGCTCATAGGACCTTC+TGG | - | chr7.3:25206802-25206821 | None:intergenic | 55.0% | |
| GCTCCCCAAATCCTCTCCAT+TGG | - | chr7.3:25206982-25207001 | None:intergenic | 55.0% | |
| GGAGCTGAAAGGTGAGACTC+TGG | + | chr7.3:25206864-25206883 | MS.gene55020:CDS | 55.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr7.3 | gene | 25206793 | 25207062 | 25206793 | ID=MS.gene55020 |
| chr7.3 | mRNA | 25206793 | 25207062 | 25206793 | ID=MS.gene55020.t1;Parent=MS.gene55020 |
| chr7.3 | exon | 25206793 | 25207062 | 25206793 | ID=MS.gene55020.t1.exon1;Parent=MS.gene55020.t1 |
| chr7.3 | CDS | 25206793 | 25207062 | 25206793 | ID=cds.MS.gene55020.t1;Parent=MS.gene55020.t1 |
| Gene Sequence |
| Protein sequence |
