Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene55042.t1 | PNX90329.1 | 85.1 | 282 | 23 | 3 | 1 | 266 | 5 | 283 | 5.90E-121 | 443.7 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene55042.t1 | Q94KL8 | 51.1 | 262 | 110 | 3 | 8 | 251 | 13 | 274 | 1.9e-67 | 257.3 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene55042.t1 | A0A2K3MHV1 | 85.1 | 282 | 23 | 3 | 1 | 266 | 5 | 283 | 4.2e-121 | 443.7 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene060998 | MS.gene55042 | 0.80855 | 2.93E-50 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene55042.t1 | MTR_7g105580 | 76.190 | 252 | 55 | 3 | 1 | 251 | 1 | 248 | 4.68e-130 | 369 |
| MS.gene55042.t1 | MTR_7g105590 | 89.119 | 193 | 4 | 2 | 1 | 177 | 1 | 192 | 3.56e-118 | 336 |
| MS.gene55042.t1 | MTR_4g052350 | 51.953 | 256 | 104 | 2 | 7 | 246 | 12 | 264 | 1.76e-82 | 248 |
| MS.gene55042.t1 | MTR_4g052300 | 51.765 | 255 | 104 | 2 | 8 | 246 | 7 | 258 | 1.07e-80 | 244 |
| MS.gene55042.t1 | MTR_4g052340 | 49.805 | 257 | 110 | 2 | 7 | 247 | 12 | 265 | 5.16e-80 | 242 |
| MS.gene55042.t1 | MTR_7g105460 | 49.804 | 255 | 112 | 1 | 8 | 246 | 12 | 266 | 2.07e-79 | 241 |
| MS.gene55042.t1 | MTR_7g071680 | 49.077 | 271 | 102 | 4 | 7 | 242 | 27 | 296 | 8.51e-79 | 240 |
| MS.gene55042.t1 | MTR_4g052320 | 50.196 | 255 | 109 | 2 | 8 | 246 | 7 | 259 | 3.37e-77 | 235 |
| MS.gene55042.t1 | MTR_7g110130 | 45.627 | 263 | 126 | 2 | 7 | 252 | 4 | 266 | 3.35e-70 | 217 |
| MS.gene55042.t1 | MTR_7g107670 | 43.542 | 271 | 130 | 4 | 1 | 249 | 25 | 294 | 1.39e-68 | 214 |
| MS.gene55042.t1 | MTR_2g090165 | 43.726 | 263 | 130 | 3 | 7 | 252 | 4 | 265 | 6.78e-62 | 196 |
| MS.gene55042.t1 | MTR_7g083870 | 42.804 | 271 | 131 | 5 | 2 | 249 | 28 | 297 | 1.80e-61 | 196 |
| MS.gene55042.t1 | MTR_6g023590 | 39.044 | 251 | 135 | 3 | 8 | 240 | 12 | 262 | 3.75e-61 | 194 |
| MS.gene55042.t1 | MTR_7g105570 | 44.398 | 241 | 99 | 1 | 6 | 246 | 18 | 223 | 1.15e-60 | 191 |
| MS.gene55042.t1 | MTR_7g110160 | 43.701 | 254 | 107 | 3 | 7 | 243 | 4 | 238 | 1.52e-60 | 192 |
| MS.gene55042.t1 | MTR_6g035395 | 43.983 | 241 | 130 | 4 | 7 | 243 | 3 | 242 | 8.58e-60 | 190 |
| MS.gene55042.t1 | MTR_3g020670 | 43.346 | 263 | 126 | 5 | 7 | 246 | 14 | 276 | 1.32e-58 | 188 |
| MS.gene55042.t1 | MTR_7g110140 | 44.000 | 250 | 122 | 4 | 8 | 240 | 5 | 253 | 2.09e-58 | 187 |
| MS.gene55042.t1 | MTR_4g078630 | 41.353 | 266 | 127 | 6 | 7 | 246 | 17 | 279 | 5.54e-57 | 184 |
| MS.gene55042.t1 | MTR_6g023610 | 40.079 | 252 | 133 | 3 | 6 | 239 | 10 | 261 | 6.61e-56 | 181 |
| MS.gene55042.t1 | MTR_6g023990 | 37.849 | 251 | 138 | 3 | 8 | 240 | 13 | 263 | 8.45e-55 | 177 |
| MS.gene55042.t1 | MTR_6g023950 | 39.442 | 251 | 134 | 3 | 8 | 240 | 12 | 262 | 2.40e-54 | 176 |
| MS.gene55042.t1 | MTR_6g023910 | 38.400 | 250 | 136 | 3 | 8 | 239 | 12 | 261 | 1.80e-53 | 174 |
| MS.gene55042.t1 | MTR_6g023600 | 37.450 | 251 | 139 | 3 | 8 | 240 | 12 | 262 | 6.15e-53 | 173 |
| MS.gene55042.t1 | MTR_6g023600 | 37.551 | 245 | 141 | 3 | 8 | 240 | 12 | 256 | 1.00e-50 | 167 |
| MS.gene55042.t1 | MTR_3g113870 | 40.097 | 207 | 102 | 3 | 17 | 202 | 29 | 234 | 3.49e-42 | 144 |
| MS.gene55042.t1 | MTR_4g084800 | 52.985 | 134 | 47 | 1 | 5 | 122 | 9 | 142 | 4.91e-41 | 139 |
| MS.gene55042.t1 | MTR_3g085740 | 27.668 | 253 | 154 | 8 | 7 | 238 | 73 | 317 | 2.35e-22 | 94.4 |
| MS.gene55042.t1 | MTR_1g051165 | 27.473 | 273 | 166 | 8 | 7 | 250 | 12 | 281 | 1.32e-21 | 91.7 |
| MS.gene55042.t1 | MTR_7g094510 | 29.779 | 272 | 140 | 6 | 1 | 239 | 1 | 254 | 2.65e-21 | 90.5 |
| MS.gene55042.t1 | MTR_4g058840 | 29.699 | 266 | 160 | 7 | 9 | 250 | 11 | 273 | 4.19e-21 | 90.1 |
| MS.gene55042.t1 | MTR_4g058820 | 29.699 | 266 | 160 | 7 | 9 | 250 | 11 | 273 | 4.19e-21 | 90.1 |
| MS.gene55042.t1 | MTR_4g132850 | 28.958 | 259 | 151 | 9 | 5 | 241 | 3 | 250 | 4.36e-21 | 89.7 |
| MS.gene55042.t1 | MTR_4g058810 | 28.947 | 266 | 162 | 7 | 9 | 250 | 11 | 273 | 2.60e-20 | 88.2 |
| MS.gene55042.t1 | MTR_8g442750 | 31.034 | 261 | 139 | 9 | 9 | 238 | 14 | 264 | 3.40e-20 | 88.2 |
| MS.gene55042.t1 | MTR_7g063320 | 28.854 | 253 | 154 | 7 | 9 | 239 | 15 | 263 | 1.69e-19 | 85.5 |
| MS.gene55042.t1 | MTR_7g113650 | 28.682 | 258 | 149 | 9 | 8 | 238 | 39 | 288 | 2.09e-19 | 85.9 |
| MS.gene55042.t1 | MTR_3g068070 | 26.415 | 265 | 169 | 5 | 2 | 245 | 6 | 265 | 4.92e-19 | 84.3 |
| MS.gene55042.t1 | MTR_1196s0010 | 29.084 | 251 | 151 | 6 | 7 | 239 | 272 | 513 | 5.35e-19 | 86.7 |
| MS.gene55042.t1 | MTR_3g068175 | 29.020 | 255 | 149 | 8 | 9 | 240 | 17 | 262 | 3.73e-18 | 82.0 |
| MS.gene55042.t1 | MTR_7g094520 | 27.969 | 261 | 146 | 4 | 9 | 239 | 11 | 259 | 8.11e-18 | 80.9 |
| MS.gene55042.t1 | MTR_1g068955 | 31.538 | 260 | 121 | 11 | 9 | 230 | 14 | 254 | 1.14e-17 | 80.9 |
| MS.gene55042.t1 | MTR_7g105560 | 63.793 | 58 | 21 | 0 | 5 | 62 | 9 | 66 | 1.21e-17 | 78.2 |
| MS.gene55042.t1 | MTR_3g068065 | 26.562 | 256 | 154 | 5 | 9 | 240 | 17 | 262 | 1.50e-17 | 80.5 |
| MS.gene55042.t1 | MTR_3g068085 | 26.984 | 252 | 158 | 6 | 9 | 240 | 15 | 260 | 2.73e-17 | 79.7 |
| MS.gene55042.t1 | MTR_1g082760 | 29.070 | 258 | 148 | 8 | 8 | 238 | 39 | 288 | 4.19e-17 | 79.3 |
| MS.gene55042.t1 | MTR_4g064887 | 25.882 | 255 | 157 | 7 | 9 | 240 | 15 | 260 | 6.62e-17 | 78.6 |
| MS.gene55042.t1 | MTR_3g068155 | 26.562 | 256 | 162 | 7 | 6 | 240 | 14 | 264 | 2.12e-16 | 77.4 |
| MS.gene55042.t1 | MTR_3g068175 | 28.571 | 259 | 149 | 9 | 9 | 240 | 17 | 266 | 4.57e-16 | 76.3 |
| MS.gene55042.t1 | MTR_7g063360 | 26.877 | 253 | 161 | 6 | 9 | 240 | 16 | 265 | 5.47e-16 | 75.9 |
| MS.gene55042.t1 | MTR_7g063270 | 25.758 | 264 | 169 | 7 | 1 | 241 | 6 | 265 | 5.04e-15 | 73.2 |
| MS.gene55042.t1 | MTR_7g063390 | 25.984 | 254 | 162 | 7 | 9 | 240 | 16 | 265 | 5.74e-15 | 73.2 |
| MS.gene55042.t1 | MTR_7g063260 | 26.316 | 266 | 166 | 8 | 1 | 241 | 6 | 266 | 6.93e-15 | 72.8 |
| MS.gene55042.t1 | MTR_7g063330 | 25.984 | 254 | 163 | 7 | 9 | 241 | 15 | 264 | 5.30e-14 | 70.5 |
| MS.gene55042.t1 | MTR_4g064887 | 27.083 | 192 | 119 | 4 | 9 | 181 | 15 | 204 | 9.81e-14 | 68.9 |
| MS.gene55042.t1 | MTR_8g096580 | 28.497 | 193 | 111 | 4 | 9 | 179 | 45 | 232 | 2.87e-13 | 68.9 |
| MS.gene55042.t1 | MTR_7g080410 | 29.381 | 194 | 112 | 4 | 9 | 179 | 38 | 229 | 6.85e-13 | 67.8 |
| MS.gene55042.t1 | MTR_7g080410 | 29.381 | 194 | 112 | 4 | 9 | 179 | 38 | 229 | 7.73e-13 | 67.4 |
| MS.gene55042.t1 | MTR_4g058830 | 31.515 | 165 | 108 | 4 | 89 | 250 | 57 | 219 | 4.08e-12 | 64.3 |
| MS.gene55042.t1 | MTR_4g064883 | 26.396 | 197 | 124 | 5 | 9 | 186 | 14 | 208 | 8.96e-11 | 60.5 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene55042.t1 | AT3G51680 | 48.689 | 267 | 105 | 4 | 7 | 241 | 30 | 296 | 9.85e-69 | 214 |
| MS.gene55042.t1 | AT1G52340 | 42.105 | 266 | 128 | 4 | 7 | 246 | 16 | 281 | 4.38e-63 | 199 |
| MS.gene55042.t1 | AT3G29250 | 44.231 | 260 | 125 | 6 | 6 | 247 | 3 | 260 | 1.72e-62 | 197 |
| MS.gene55042.t1 | AT4G03140 | 43.529 | 255 | 124 | 2 | 7 | 241 | 76 | 330 | 3.99e-62 | 199 |
| MS.gene55042.t1 | AT3G29250 | 44.231 | 260 | 125 | 6 | 6 | 247 | 39 | 296 | 8.02e-62 | 197 |
| MS.gene55042.t1 | AT2G47130 | 43.874 | 253 | 121 | 5 | 6 | 240 | 3 | 252 | 1.07e-61 | 195 |
| MS.gene55042.t1 | AT2G47140 | 41.667 | 252 | 129 | 3 | 6 | 240 | 3 | 253 | 3.49e-61 | 194 |
| MS.gene55042.t1 | AT3G26770 | 41.961 | 255 | 127 | 3 | 7 | 240 | 39 | 293 | 9.17e-61 | 194 |
| MS.gene55042.t1 | AT3G29250 | 43.969 | 257 | 124 | 6 | 9 | 247 | 44 | 298 | 2.20e-60 | 193 |
| MS.gene55042.t1 | AT2G47120 | 41.245 | 257 | 131 | 6 | 6 | 245 | 3 | 256 | 6.91e-60 | 191 |
| MS.gene55042.t1 | AT2G47120 | 41.176 | 255 | 130 | 6 | 8 | 245 | 6 | 257 | 1.57e-59 | 190 |
| MS.gene55042.t1 | AT3G29260 | 41.245 | 257 | 132 | 4 | 6 | 245 | 3 | 257 | 5.16e-59 | 188 |
| MS.gene55042.t1 | AT3G26760 | 40.613 | 261 | 124 | 4 | 7 | 240 | 34 | 290 | 1.37e-57 | 186 |
| MS.gene55042.t1 | AT3G42960 | 41.045 | 268 | 126 | 9 | 7 | 246 | 6 | 269 | 1.41e-47 | 159 |
| MS.gene55042.t1 | AT2G47150 | 30.172 | 232 | 123 | 5 | 9 | 239 | 1 | 194 | 2.96e-26 | 102 |
| MS.gene55042.t1 | AT5G18210 | 31.801 | 261 | 139 | 7 | 9 | 239 | 8 | 259 | 4.10e-23 | 95.5 |
| MS.gene55042.t1 | AT3G03980 | 29.615 | 260 | 150 | 4 | 9 | 239 | 14 | 269 | 1.08e-21 | 91.7 |
| MS.gene55042.t1 | AT3G04000 | 28.044 | 271 | 139 | 5 | 9 | 238 | 14 | 269 | 7.40e-20 | 86.7 |
| MS.gene55042.t1 | AT5G18210 | 30.375 | 293 | 153 | 10 | 1 | 263 | 1 | 272 | 1.54e-19 | 85.9 |
| MS.gene55042.t1 | AT2G17845 | 25.912 | 274 | 163 | 8 | 9 | 250 | 47 | 312 | 1.82e-19 | 86.3 |
| MS.gene55042.t1 | AT5G06060 | 27.626 | 257 | 162 | 5 | 9 | 246 | 9 | 260 | 6.21e-19 | 84.0 |
| MS.gene55042.t1 | AT2G29330 | 26.820 | 261 | 159 | 7 | 9 | 246 | 7 | 258 | 7.90e-19 | 84.0 |
| MS.gene55042.t1 | AT1G63380 | 26.217 | 267 | 169 | 6 | 9 | 250 | 22 | 285 | 1.42e-18 | 83.6 |
| MS.gene55042.t1 | AT1G63380 | 26.217 | 267 | 169 | 6 | 9 | 250 | 15 | 278 | 1.47e-18 | 83.6 |
| MS.gene55042.t1 | AT1G63380 | 26.217 | 267 | 169 | 6 | 9 | 250 | 17 | 280 | 1.51e-18 | 83.6 |
| MS.gene55042.t1 | AT1G63380 | 26.217 | 267 | 169 | 6 | 9 | 250 | 19 | 282 | 1.57e-18 | 83.2 |
| MS.gene55042.t1 | AT3G12800 | 30.534 | 262 | 140 | 10 | 9 | 238 | 10 | 261 | 2.14e-18 | 83.2 |
| MS.gene55042.t1 | AT1G62610 | 26.217 | 267 | 169 | 6 | 9 | 250 | 14 | 277 | 2.63e-18 | 82.8 |
| MS.gene55042.t1 | AT1G62610 | 26.217 | 267 | 169 | 6 | 9 | 250 | 15 | 278 | 2.69e-18 | 82.8 |
| MS.gene55042.t1 | AT1G62610 | 26.217 | 267 | 169 | 6 | 9 | 250 | 19 | 282 | 2.96e-18 | 82.4 |
| MS.gene55042.t1 | AT1G62610 | 26.217 | 267 | 169 | 6 | 9 | 250 | 13 | 276 | 3.04e-18 | 82.4 |
| MS.gene55042.t1 | AT2G29340 | 25.287 | 261 | 163 | 6 | 9 | 246 | 7 | 258 | 4.93e-18 | 81.6 |
| MS.gene55042.t1 | AT1G54870 | 31.100 | 209 | 114 | 7 | 8 | 190 | 83 | 287 | 5.75e-18 | 82.4 |
| MS.gene55042.t1 | AT1G54870 | 31.100 | 209 | 114 | 7 | 8 | 190 | 81 | 285 | 5.98e-18 | 82.4 |
| MS.gene55042.t1 | AT2G29340 | 25.287 | 261 | 163 | 6 | 9 | 246 | 7 | 258 | 9.61e-18 | 81.6 |
| MS.gene55042.t1 | AT3G55310 | 25.940 | 266 | 170 | 5 | 9 | 250 | 17 | 279 | 1.52e-17 | 80.5 |
| MS.gene55042.t1 | AT4G05530 | 26.996 | 263 | 159 | 8 | 1 | 241 | 1 | 252 | 1.13e-16 | 77.8 |
| MS.gene55042.t1 | AT2G29310 | 25.188 | 266 | 157 | 7 | 9 | 246 | 7 | 258 | 1.94e-16 | 77.4 |
| MS.gene55042.t1 | AT3G55290 | 25.188 | 266 | 172 | 5 | 9 | 250 | 18 | 280 | 2.07e-16 | 77.4 |
| MS.gene55042.t1 | AT3G55290 | 25.188 | 266 | 172 | 5 | 9 | 250 | 17 | 279 | 2.11e-16 | 77.4 |
| MS.gene55042.t1 | AT1G07440 | 26.459 | 257 | 164 | 7 | 9 | 246 | 12 | 262 | 2.46e-16 | 77.0 |
| MS.gene55042.t1 | AT3G05260 | 25.969 | 258 | 155 | 7 | 8 | 238 | 36 | 284 | 6.57e-16 | 76.3 |
| MS.gene55042.t1 | AT3G46170 | 25.940 | 266 | 170 | 5 | 9 | 250 | 26 | 288 | 2.04e-15 | 74.7 |
| MS.gene55042.t1 | AT2G29340 | 26.768 | 198 | 124 | 4 | 9 | 187 | 7 | 202 | 4.16e-15 | 72.4 |
| MS.gene55042.t1 | AT2G29340 | 26.768 | 198 | 124 | 4 | 9 | 187 | 7 | 202 | 4.16e-15 | 72.4 |
| MS.gene55042.t1 | AT2G30670 | 26.054 | 261 | 161 | 7 | 9 | 246 | 7 | 258 | 5.56e-15 | 73.2 |
| MS.gene55042.t1 | AT1G07450 | 26.070 | 257 | 165 | 7 | 9 | 246 | 8 | 258 | 1.96e-14 | 71.6 |
| MS.gene55042.t1 | AT2G29360 | 26.070 | 257 | 166 | 6 | 9 | 246 | 16 | 267 | 5.95e-14 | 70.5 |
| MS.gene55042.t1 | AT1G24360 | 23.922 | 255 | 165 | 7 | 7 | 240 | 72 | 318 | 6.81e-14 | 70.9 |
| MS.gene55042.t1 | AT4G13180 | 27.165 | 254 | 157 | 6 | 9 | 239 | 13 | 261 | 7.98e-14 | 70.1 |
| MS.gene55042.t1 | AT2G29320 | 24.809 | 262 | 164 | 7 | 9 | 246 | 13 | 265 | 1.96e-13 | 68.9 |
| MS.gene55042.t1 | AT2G29310 | 27.083 | 192 | 119 | 4 | 9 | 181 | 7 | 196 | 1.15e-12 | 65.5 |
| MS.gene55042.t1 | AT2G29370 | 26.459 | 257 | 165 | 7 | 9 | 246 | 16 | 267 | 1.05e-11 | 63.9 |
| MS.gene55042.t1 | AT2G29300 | 23.346 | 257 | 172 | 7 | 9 | 245 | 7 | 258 | 1.45e-11 | 63.5 |
| MS.gene55042.t1 | AT2G29350 | 26.744 | 258 | 162 | 8 | 9 | 246 | 15 | 265 | 1.72e-11 | 63.2 |
| MS.gene55042.t1 | AT2G29300 | 22.878 | 271 | 179 | 8 | 9 | 254 | 7 | 272 | 1.92e-11 | 63.5 |
| MS.gene55042.t1 | AT5G50600 | 26.396 | 197 | 121 | 6 | 1 | 177 | 37 | 229 | 1.98e-11 | 63.9 |
| MS.gene55042.t1 | AT5G50700 | 26.396 | 197 | 121 | 6 | 1 | 177 | 37 | 229 | 1.98e-11 | 63.9 |
| MS.gene55042.t1 | AT5G10050 | 27.604 | 192 | 121 | 5 | 10 | 186 | 6 | 194 | 2.10e-11 | 63.2 |
| MS.gene55042.t1 | AT1G49670 | 28.788 | 198 | 111 | 5 | 11 | 179 | 6 | 202 | 5.36e-11 | 63.2 |
| MS.gene55042.t1 | AT1G49670 | 28.788 | 198 | 111 | 5 | 11 | 179 | 6 | 202 | 5.91e-11 | 62.8 |
| MS.gene55042.t1 | AT5G50690 | 28.947 | 190 | 113 | 6 | 7 | 177 | 43 | 229 | 8.42e-11 | 61.2 |
| MS.gene55042.t1 | AT5G50590 | 28.947 | 190 | 113 | 6 | 7 | 177 | 43 | 229 | 8.42e-11 | 61.2 |
Find 46 sgRNAs with CRISPR-Local
Find 54 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| GTATTTGATGTTAATGTTTA+TGG | 0.126315 | 7.2:-24755615 | MS.gene55042:CDS |
| TCTTCCATGGCTTCAATTAT+TGG | 0.161831 | 7.2:-24756043 | None:intergenic |
| AAGAATTTGTGTGTAGAATT+AGG | 0.223231 | 7.2:-24755444 | MS.gene55042:CDS |
| GATACCGTAGTTTCGAATTA+TGG | 0.242135 | 7.2:-24755726 | MS.gene55042:intron |
| TGTGTAGAATTAGGACAATA+TGG | 0.323778 | 7.2:-24755435 | MS.gene55042:CDS |
| TAATGTTTATGGAGCTTTCT+TGG | 0.326854 | 7.2:-24755604 | MS.gene55042:CDS |
| TTCAGTGCATTGGTTAGAAT+TGG | 0.330425 | 7.2:+24755372 | None:intergenic |
| GAATGGAGTCAACTTTGTTT+TGG | 0.335482 | 7.2:-24755226 | MS.gene55042:CDS |
| CTCTCTTTGTGCAGGCTTGA+AGG | 0.373704 | 7.2:-24755948 | MS.gene55042:intron |
| ACAGGAGGTGCTAGTGGAAT+TGG | 0.390234 | 7.2:-24755909 | MS.gene55042:CDS |
| CAATCCCTTTGCAATGAACT+TGG | 0.395230 | 7.2:-24755813 | MS.gene55042:CDS |
| AATGTTTATGGAGCTTTCTT+GGG | 0.395810 | 7.2:-24755603 | MS.gene55042:CDS |
| TATCATAATTTAATATTTCA+CGG | 0.396520 | 7.2:+24755655 | None:intergenic |
| AAATGTTTGATGAATGGTTA+TGG | 0.429070 | 7.2:-24755156 | MS.gene55042:CDS |
| ATGATAGCGAAGCTTTCAAA+AGG | 0.448243 | 7.2:-24755638 | MS.gene55042:CDS |
| CTTGTTCATATTCAGTGCAT+TGG | 0.455778 | 7.2:+24755362 | None:intergenic |
| TGGCTTCAATTATTGGCAAA+AGG | 0.456299 | 7.2:-24756036 | MS.gene55042:intron |
| GACATCATGTTTAACAATGC+TGG | 0.458821 | 7.2:-24755696 | MS.gene55042:CDS |
| GAATATGAACAAGAGTGAAA+TGG | 0.462229 | 7.2:-24755352 | MS.gene55042:CDS |
| TCTTCCAAGTTCATTGCAAA+GGG | 0.463133 | 7.2:+24755809 | None:intergenic |
| TTCTTCCAAGTTCATTGCAA+AGG | 0.472083 | 7.2:+24755808 | None:intergenic |
| GATGCTTCAAAGTTTGTGAA+TGG | 0.492301 | 7.2:-24755243 | MS.gene55042:CDS |
| TGCAGAAGTGAATGACATAT+TGG | 0.498711 | 7.2:+24755185 | None:intergenic |
| TGGGTGCTAAGCATGCTGCT+AGG | 0.498748 | 7.2:-24755584 | MS.gene55042:CDS |
| GTGTAGAATTAGGACAATAT+GGG | 0.500905 | 7.2:-24755434 | MS.gene55042:CDS |
| GGGTGCTAAGCATGCTGCTA+GGG | 0.521675 | 7.2:-24755583 | MS.gene55042:CDS |
| AGAGGCTATTCTTGAAGTTG+AGG | 0.532968 | 7.2:-24755298 | MS.gene55042:CDS |
| CTACTGATCAATGTGCTTGG+TGG | 0.533586 | 7.2:+24755131 | None:intergenic |
| TTTGCCAATAATTGAAGCCA+TGG | 0.539515 | 7.2:+24756039 | None:intergenic |
| TTCTTCAAAGCATGCATCAG+TGG | 0.545758 | 7.2:-24755475 | MS.gene55042:CDS |
| GGTAAAGTAGCAATAATCAC+AGG | 0.547973 | 7.2:-24755927 | MS.gene55042:CDS |
| TCTTCAAAGCATGCATCAGT+GGG | 0.552690 | 7.2:-24755474 | MS.gene55042:CDS |
| TTACTACTGATCAATGTGCT+TGG | 0.561074 | 7.2:+24755128 | None:intergenic |
| ATAATCACAGGAGGTGCTAG+TGG | 0.563533 | 7.2:-24755915 | MS.gene55042:CDS |
| GCAGATATTCAAGATGAAGT+AGG | 0.564586 | 7.2:-24755837 | MS.gene55042:CDS |
| CTCAGTTGGTGTGTTGAAAG+AGG | 0.576270 | 7.2:-24755316 | MS.gene55042:CDS |
| TGATAGCGAAGCTTTCAAAA+GGG | 0.590961 | 7.2:-24755637 | MS.gene55042:CDS |
| GCATTGGTTAGAATTGGAGT+AGG | 0.601696 | 7.2:+24755378 | None:intergenic |
| ATTGGAGTAGGAATAGCTCC+TGG | 0.611949 | 7.2:+24755390 | None:intergenic |
| AAAGTGAATTGTATCTCACC+AGG | 0.618157 | 7.2:-24755408 | MS.gene55042:CDS |
| CTCTTTCAACACACCAACTG+AGG | 0.623548 | 7.2:+24755317 | None:intergenic |
| TGAAGTTGAGGATATAGCTG+AGG | 0.641258 | 7.2:-24755286 | MS.gene55042:CDS |
| GCTAAACTATTTGTTCAACA+TGG | 0.643126 | 7.2:-24755876 | MS.gene55042:CDS |
| AGTGTTGCTACAGAAACTGC+TGG | 0.652960 | 7.2:-24755519 | MS.gene55042:CDS |
| AGAAGTGAATGACATATTGG+TGG | 0.654410 | 7.2:+24755188 | None:intergenic |
| AAAGTAGCAATAATCACAGG+AGG | 0.676889 | 7.2:-24755924 | MS.gene55042:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | TATCATAATTTAATATTTCA+CGG | + | chr7.2:24755516-24755535 | None:intergenic | 10.0% |
| !! | CTTAAAAAATGTTTGATGAA+TGG | - | chr7.2:24756006-24756025 | MS.gene55042:intron | 20.0% |
| !! | GTATTTGATGTTAATGTTTA+TGG | - | chr7.2:24755553-24755572 | MS.gene55042:CDS | 20.0% |
| ! | AAATGTTTGATGAATGGTTA+TGG | - | chr7.2:24756012-24756031 | MS.gene55042:intron | 25.0% |
| ! | AAGAATTTGTGTGTAGAATT+AGG | - | chr7.2:24755724-24755743 | MS.gene55042:intron | 25.0% |
| ! | TGAATGAGACATAAAAAATG+TGG | - | chr7.2:24755417-24755436 | MS.gene55042:CDS | 25.0% |
| !!! | AGAATAACACCTTTTTTGTT+TGG | + | chr7.2:24755619-24755638 | None:intergenic | 25.0% |
| !!! | GAATAACACCTTTTTTGTTT+GGG | + | chr7.2:24755618-24755637 | None:intergenic | 25.0% |
| AATGTTTATGGAGCTTTCTT+GGG | - | chr7.2:24755565-24755584 | MS.gene55042:CDS | 30.0% | |
| CTTACCATAATTCGAAACTA+CGG | + | chr7.2:24755449-24755468 | None:intergenic | 30.0% | |
| GAATATGAACAAGAGTGAAA+TGG | - | chr7.2:24755816-24755835 | MS.gene55042:CDS | 30.0% | |
| GCTAAACTATTTGTTCAACA+TGG | - | chr7.2:24755292-24755311 | MS.gene55042:CDS | 30.0% | |
| GTAATGATCCCAAACAAAAA+AGG | - | chr7.2:24755607-24755626 | MS.gene55042:CDS | 30.0% | |
| GTGTAGAATTAGGACAATAT+GGG | - | chr7.2:24755734-24755753 | MS.gene55042:intron | 30.0% | |
| TAATGTTTATGGAGCTTTCT+TGG | - | chr7.2:24755564-24755583 | MS.gene55042:CDS | 30.0% | |
| TGTGTAGAATTAGGACAATA+TGG | - | chr7.2:24755733-24755752 | MS.gene55042:intron | 30.0% | |
| AAAGTAGCAATAATCACAGG+AGG | - | chr7.2:24755244-24755263 | MS.gene55042:CDS | 35.0% | |
| AAAGTGAATTGTATCTCACC+AGG | - | chr7.2:24755760-24755779 | MS.gene55042:intron | 35.0% | |
| AGAAGTGAATGACATATTGG+TGG | + | chr7.2:24755983-24756002 | None:intergenic | 35.0% | |
| CTTGTTCATATTCAGTGCAT+TGG | + | chr7.2:24755809-24755828 | None:intergenic | 35.0% | |
| GACATCATGTTTAACAATGC+TGG | - | chr7.2:24755472-24755491 | MS.gene55042:CDS | 35.0% | |
| GATACCGTAGTTTCGAATTA+TGG | - | chr7.2:24755442-24755461 | MS.gene55042:CDS | 35.0% | |
| GATGCTTCAAAGTTTGTGAA+TGG | - | chr7.2:24755925-24755944 | MS.gene55042:CDS | 35.0% | |
| GCAGATATTCAAGATGAAGT+AGG | - | chr7.2:24755331-24755350 | MS.gene55042:CDS | 35.0% | |
| GGCAAAAGGTAACTATATTG+TGG | - | chr7.2:24755146-24755165 | MS.gene55042:CDS | 35.0% | |
| GGTAAAGTAGCAATAATCAC+AGG | - | chr7.2:24755241-24755260 | MS.gene55042:CDS | 35.0% | |
| TCTTCCAAGTTCATTGCAAA+GGG | + | chr7.2:24755362-24755381 | None:intergenic | 35.0% | |
| TGCAGAAGTGAATGACATAT+TGG | + | chr7.2:24755986-24756005 | None:intergenic | 35.0% | |
| TGGCTTCAATTATTGGCAAA+AGG | - | chr7.2:24755132-24755151 | MS.gene55042:CDS | 35.0% | |
| TTCTTCCAAGTTCATTGCAA+AGG | + | chr7.2:24755363-24755382 | None:intergenic | 35.0% | |
| ! | ATGATAGCGAAGCTTTCAAA+AGG | - | chr7.2:24755530-24755549 | MS.gene55042:CDS | 35.0% |
| ! | TGATAGCGAAGCTTTCAAAA+GGG | - | chr7.2:24755531-24755550 | MS.gene55042:CDS | 35.0% |
| !! | GAATGGAGTCAACTTTGTTT+TGG | - | chr7.2:24755942-24755961 | MS.gene55042:intron | 35.0% |
| !! | TTCAGTGCATTGGTTAGAAT+TGG | + | chr7.2:24755799-24755818 | None:intergenic | 35.0% |
| AGAGGCTATTCTTGAAGTTG+AGG | - | chr7.2:24755870-24755889 | MS.gene55042:CDS | 40.0% | |
| CAAGTAATCTCTCTTTGTGC+AGG | - | chr7.2:24755212-24755231 | MS.gene55042:CDS | 40.0% | |
| CAATCCCTTTGCAATGAACT+TGG | - | chr7.2:24755355-24755374 | MS.gene55042:CDS | 40.0% | |
| TCTTCAAAGCATGCATCAGT+GGG | - | chr7.2:24755694-24755713 | MS.gene55042:CDS | 40.0% | |
| TGAAGTTGAGGATATAGCTG+AGG | - | chr7.2:24755882-24755901 | MS.gene55042:CDS | 40.0% | |
| TTCTTCAAAGCATGCATCAG+TGG | - | chr7.2:24755693-24755712 | MS.gene55042:CDS | 40.0% | |
| !! | GAGAAAGTTTTGTCCTCAGT+TGG | - | chr7.2:24755838-24755857 | MS.gene55042:CDS | 40.0% |
| !! | GCATTGGTTAGAATTGGAGT+AGG | + | chr7.2:24755793-24755812 | None:intergenic | 40.0% |
| !!! | GGAGTCAACTTTGTTTTGGA+TGG | - | chr7.2:24755946-24755965 | MS.gene55042:intron | 40.0% |
| !!! | GTCAACTTTGTTTTGGATGG+AGG | - | chr7.2:24755949-24755968 | MS.gene55042:intron | 40.0% |
| ATTGGAGTAGGAATAGCTCC+TGG | + | chr7.2:24755781-24755800 | None:intergenic | 45.0% | |
| CTACTGATCAATGTGCTTGG+TGG | + | chr7.2:24756040-24756059 | None:intergenic | 45.0% | |
| CTCTTTCAACACACCAACTG+AGG | + | chr7.2:24755854-24755873 | None:intergenic | 45.0% | |
| ! | AGTGTTGCTACAGAAACTGC+TGG | - | chr7.2:24755649-24755668 | MS.gene55042:CDS | 45.0% |
| ! | CTCAGTTGGTGTGTTGAAAG+AGG | - | chr7.2:24755852-24755871 | MS.gene55042:CDS | 45.0% |
| !! | ATAATCACAGGAGGTGCTAG+TGG | - | chr7.2:24755253-24755272 | MS.gene55042:CDS | 45.0% |
| CTCTCTTTGTGCAGGCTTGA+AGG | - | chr7.2:24755220-24755239 | MS.gene55042:CDS | 50.0% | |
| !! | ACAGGAGGTGCTAGTGGAAT+TGG | - | chr7.2:24755259-24755278 | MS.gene55042:CDS | 50.0% |
| GGGTGCTAAGCATGCTGCTA+GGG | - | chr7.2:24755585-24755604 | MS.gene55042:CDS | 55.0% | |
| TGGGTGCTAAGCATGCTGCT+AGG | - | chr7.2:24755584-24755603 | MS.gene55042:CDS | 55.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr7.2 | gene | 24755131 | 24756059 | 24755131 | ID=MS.gene55042 |
| chr7.2 | mRNA | 24755131 | 24756059 | 24755131 | ID=MS.gene55042.t1;Parent=MS.gene55042 |
| chr7.2 | exon | 24756037 | 24756059 | 24756037 | ID=MS.gene55042.t1.exon1;Parent=MS.gene55042.t1 |
| chr7.2 | CDS | 24756037 | 24756059 | 24756037 | ID=cds.MS.gene55042.t1;Parent=MS.gene55042.t1 |
| chr7.2 | exon | 24755787 | 24755956 | 24755787 | ID=MS.gene55042.t1.exon2;Parent=MS.gene55042.t1 |
| chr7.2 | CDS | 24755787 | 24755956 | 24755787 | ID=cds.MS.gene55042.t1;Parent=MS.gene55042.t1 |
| chr7.2 | exon | 24755131 | 24755738 | 24755131 | ID=MS.gene55042.t1.exon3;Parent=MS.gene55042.t1 |
| chr7.2 | CDS | 24755131 | 24755738 | 24755131 | ID=cds.MS.gene55042.t1;Parent=MS.gene55042.t1 |
| Gene Sequence |
| Protein sequence |
