AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene55360

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score

Find 79 sgRNAs with CRISPR-Local

Find 145 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
AAAGGGAAGGTTGAAATCTT+TGG 0.253458 5.2:-33323041 MS.gene55360:CDS
TTCCTTGAAGTCTTCCTAAT+TGG 0.258180 5.2:+33323737 None:intergenic
TTTGTTAGGGTACAACTTAC+TGG 0.294670 5.2:-33324817 None:intergenic
GGCAGTGAAGGAGGAGCTTT+TGG 0.312565 5.2:+33323773 None:intergenic
CCCTGCACTATCAATGAAAA+AGG 0.317899 5.2:-33323834 MS.gene55360:CDS
GTCAGCTCTCTTTGAGGGTC+AGG 0.337676 5.2:+33323713 None:intergenic
AGTATATCCAAGCGATTATC+AGG 0.348650 5.2:-33324027 MS.gene55360:intron
CGTCACTACCATCACCAATT+AGG 0.351450 5.2:-33323751 MS.gene55360:CDS
ACGGGGAAGGAAGAAATCTT+TGG 0.373980 5.2:-33322939 MS.gene55360:CDS
GTACCTTTGTTTGATCTTGT+CGG 0.377983 5.2:+33323589 None:intergenic
ATCTTTGCTTCTCAAACTCC+TGG 0.384252 5.2:+33322728 None:intergenic
TACCTTTGTTTGATCTTGTC+GGG 0.388889 5.2:+33323590 None:intergenic
CGATAGAGTTGATAGATTCC+TGG 0.389860 5.2:-33323430 MS.gene55360:CDS
CATTGATAGTGCAGGGGTAA+TGG 0.391128 5.2:+33323841 None:intergenic
TTGCTTCATTAGCTTGCGTT+TGG 0.400523 5.2:+33323385 None:intergenic
AATGAAAAGTTACAAGCAAA+AGG 0.407225 5.2:-33322836 MS.gene55360:CDS
TGAGAACCCGGAGGCCTCAT+TGG 0.408314 5.2:-33324103 MS.gene55360:CDS
TATCTTCTTCTTGTCTTCCC+AGG 0.408442 5.2:+33323412 None:intergenic
ATTGCAGAGTGAACTTGAAC+GGG 0.409595 5.2:-33322957 MS.gene55360:CDS
ATTGCAGAGTGAACTTGAAA+AGG 0.417996 5.2:-33323059 MS.gene55360:intron
AAATCTTCAAAGTCCCTTCT+CGG 0.422429 5.2:-33324051 MS.gene55360:CDS
TTGTAGTCAGCTCTCTTTGA+GGG 0.424701 5.2:+33323708 None:intergenic
AAACAGCTTTCAAGTAAAAC+AGG 0.432687 5.2:-33324219 MS.gene55360:CDS
ACCTTGGCCAATGAGGCCTC+CGG 0.441700 5.2:+33324096 None:intergenic
CAAAGAGAGCTGACTACAAC+AGG 0.441809 5.2:-33323704 MS.gene55360:CDS
AAGGCAGCTTTCCACAGCCT+TGG 0.447928 5.2:+33322862 None:intergenic
TTGCAGAGTGAACTTGAAAA+GGG 0.452559 5.2:-33323058 MS.gene55360:intron
TCTTCAATTAACTCCGCACT+TGG 0.453600 5.2:+33322797 None:intergenic
CCTTGGCCAATGAGGCCTCC+GGG 0.464827 5.2:+33324097 None:intergenic
GGGAGACAAGTGCAGATGAA+TGG 0.466604 5.2:-33323670 MS.gene55360:CDS
AGAGTTAATTGACAAATATC+CGG 0.468352 5.2:-33322777 MS.gene55360:CDS
CTACAACAGGATCAAACAAT+GGG 0.469067 5.2:-33323691 MS.gene55360:CDS
GTTGTAGTCAGCTCTCTTTG+AGG 0.470341 5.2:+33323707 None:intergenic
ATATTTACCTGATAATCGCT+TGG 0.479753 5.2:+33324020 None:intergenic
GAGGAGAACAAAGCTGATCT+TGG 0.481626 5.2:-33324790 MS.gene55360:CDS
TGATAACGACTACATAGAAA+TGG 0.483998 5.2:-33324193 MS.gene55360:CDS
ACGCAAGCTAATGAAGCAAC+AGG 0.492493 5.2:-33323381 MS.gene55360:intron
GGAGACAAGTGCAGATGAAT+GGG 0.493349 5.2:-33323669 MS.gene55360:CDS
CGGAGCGAGAGCCAAGGCTG+AGG 0.494588 5.2:-33322993 MS.gene55360:CDS
CACCAATTAGGAAGACTTCA+AGG 0.497206 5.2:-33323739 MS.gene55360:CDS
AACATTACCTTTGAAAGACT+TGG 0.500839 5.2:+33324732 None:intergenic
CAGAGTGAGAGCCAAGGCTG+TGG 0.508607 5.2:-33322873 MS.gene55360:CDS
TGAGCAGAAGAGTGAGAACC+CGG 0.518079 5.2:-33324115 MS.gene55360:CDS
AGTTGTAGAAGAAAAGAAAC+CGG 0.524878 5.2:-33323870 MS.gene55360:CDS
ATGAAAAGTTACAAGCAAAA+GGG 0.529836 5.2:-33322835 MS.gene55360:CDS
GAAGTCTTCCTAATTGGTGA+TGG 0.542948 5.2:+33323743 None:intergenic
GATAGAGTTGATAGATTCCT+GGG 0.545013 5.2:-33323429 MS.gene55360:CDS
AGAAGAAAAGAAACCGGAAA+AGG 0.548483 5.2:-33323864 MS.gene55360:CDS
CAGAAAACCAAGTCTTTCAA+AGG 0.551506 5.2:-33324739 MS.gene55360:intron
ATCTTGGTGCCACAAGGGAT+CGG 0.552116 5.2:-33324774 MS.gene55360:CDS
AATTGCAGAGTGAACTTGAA+CGG 0.553990 5.2:-33322958 MS.gene55360:CDS
GATCGACAAGATCAAACAAT+GGG 0.555882 5.2:-33323636 MS.gene55360:CDS
CCCGGAGGCCTCATTGGCCA+AGG 0.558107 5.2:-33324097 MS.gene55360:CDS
AAGCTGATCTTGGTGCCACA+AGG 0.575606 5.2:-33324780 MS.gene55360:CDS
TAGGGTACAACTTACTGGTA+CGG 0.577931 5.2:-33324812 MS.gene55360:CDS
AGAGTGAACTTGAACGGGGA+AGG 0.580311 5.2:-33322952 MS.gene55360:CDS
GGTAGTGACGGCAGTGAAGG+AGG 0.587583 5.2:+33323764 None:intergenic
CTAATTGGTGATGGTAGTGA+CGG 0.596804 5.2:+33323752 None:intergenic
GCTTGGATATACTACCGAGA+AGG 0.608566 5.2:+33324037 None:intergenic
TATGTAAATCTAATTGCAGA+CGG 0.610209 5.2:-33323013 MS.gene55360:CDS
AGAGTGAACTTGAAAAGGGA+AGG 0.614135 5.2:-33323054 MS.gene55360:CDS
AGCTGATCTTGGTGCCACAA+GGG 0.616690 5.2:-33324779 MS.gene55360:CDS
ACTACAACAGGATCAAACAA+TGG 0.620345 5.2:-33323692 MS.gene55360:CDS
GATGGTAGTGACGGCAGTGA+AGG 0.622090 5.2:+33323761 None:intergenic
CTTGGATATACTACCGAGAA+GGG 0.626044 5.2:+33324038 None:intergenic
GTTCTCACTCTTCTGCTCAG+AGG 0.636166 5.2:+33324119 None:intergenic
ATTGGCCAAGGTCAAAAGCA+TGG 0.638135 5.2:-33324085 MS.gene55360:CDS
GGCAAGTACAATTCCAAGTG+CGG 0.640534 5.2:-33322810 MS.gene55360:CDS
TGCAGACAGAGTGAGAGCCA+AGG 0.641925 5.2:-33322879 MS.gene55360:CDS
GGTACAACTTACTGGTACGG+AGG 0.642305 5.2:-33324809 MS.gene55360:CDS
AAAGTTACAAGCAAAAGGGA+AGG 0.645842 5.2:-33322831 MS.gene55360:CDS
TGATCGACAAGATCAAACAA+TGG 0.655153 5.2:-33323637 MS.gene55360:CDS
TGCAGACGGAGCGAGAGCCA+AGG 0.657239 5.2:-33322999 MS.gene55360:CDS
GACTACATAGAAATGGTGCA+TGG 0.661525 5.2:-33324186 MS.gene55360:CDS
AGCCCGACAAGATCAAACAA+AGG 0.719711 5.2:-33323592 MS.gene55360:intron
GCAGAAGAGTGAGAACCCGG+AGG 0.721846 5.2:-33324112 MS.gene55360:CDS
GGCCAAGGTCAAAAGCATGG+TGG 0.722518 5.2:-33324082 MS.gene55360:CDS
TTGCAGAGTGAACTTGAACG+GGG 0.735768 5.2:-33322956 MS.gene55360:CDS
TACAACAGGATCAAACAATG+GGG 0.783663 5.2:-33323690 MS.gene55360:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! AAGTTGGATATATTATATAT+TGG + chr5.2:33322893-33322912 None:intergenic 15.0%
!!! TTTGTCAATTTTTTTGTAAA+TGG + chr5.2:33324797-33324816 None:intergenic 15.0%
!! AAAAAAATTGACAAATATCC+AGG - chr5.2:33324802-33324821 MS.gene55360:CDS 20.0%
!! TGGTAATTATGATTCATAAA+TGG + chr5.2:33323568-33323587 None:intergenic 20.0%
!!! ATGAAATAGACTCATTTTTA+TGG + chr5.2:33323048-33323067 None:intergenic 20.0%
!!! CTTGTAACTTTTCATTTTTA+AGG + chr5.2:33324708-33324727 None:intergenic 20.0%
!!! GGATATATTATATATTGGTT+CGG + chr5.2:33322888-33322907 None:intergenic 20.0%
!!! GTTTTTTTACTTAAGAGTAA+TGG + chr5.2:33323588-33323607 None:intergenic 20.0%
! AAAAAAAATTATGCCATGCA+TGG - chr5.2:33323141-33323160 MS.gene55360:intron 25.0%
! AAGCATTTACCATAAAAATG+AGG - chr5.2:33323085-33323104 MS.gene55360:intron 25.0%
! AATGAAAAGTTACAAGCAAA+AGG - chr5.2:33324712-33324731 MS.gene55360:intron 25.0%
! AATGATAAAAAAATAGAGGC+CGG - chr5.2:33324402-33324421 MS.gene55360:intron 25.0%
! ACATTATCTGATTATAGGTA+AGG + chr5.2:33324271-33324290 None:intergenic 25.0%
! AGAGTTAATTGACAAATATC+CGG - chr5.2:33324771-33324790 MS.gene55360:CDS 25.0%
! ATGAAAAGTTACAAGCAAAA+GGG - chr5.2:33324713-33324732 MS.gene55360:intron 25.0%
! ATTATGATTCATAAATGGAG+TGG + chr5.2:33323563-33323582 None:intergenic 25.0%
! CACTAAATGAATAAGATCTA+AGG + chr5.2:33324220-33324239 None:intergenic 25.0%
! CTAGAACATTATCTGATTAT+AGG + chr5.2:33324276-33324295 None:intergenic 25.0%
! CTATAATCAGATAATGTTCT+AGG - chr5.2:33324274-33324293 MS.gene55360:intron 25.0%
! CTGGAATGATAAAAAAATAG+AGG - chr5.2:33324398-33324417 MS.gene55360:intron 25.0%
! TACTAATGAAAAACATTGTC+AGG - chr5.2:33324085-33324104 MS.gene55360:CDS 25.0%
! TATGTAAATCTAATTGCAGA+CGG - chr5.2:33324535-33324554 MS.gene55360:intron 25.0%
!!! GTTTCTTTTCCTCATTTTTA+TGG + chr5.2:33323097-33323116 None:intergenic 25.0%
!!! TCAATTTTTTTGTAAATGGC+CGG + chr5.2:33324793-33324812 None:intergenic 25.0%
AGTTGTAGAAGAAAAGAAAC+CGG - chr5.2:33323678-33323697 MS.gene55360:CDS 30.0%
ATATTTACCTGATAATCGCT+TGG + chr5.2:33323531-33323550 None:intergenic 30.0%
ATGATAAAAAAATAGAGGCC+GGG - chr5.2:33324403-33324422 MS.gene55360:intron 30.0%
ATTTAGTGCTTTCTTTAAGC+AGG - chr5.2:33324232-33324251 MS.gene55360:CDS 30.0%
TCTAGTATGAACAATAAGTC+TGG - chr5.2:33324379-33324398 MS.gene55360:intron 30.0%
! AAACAGCTTTCAAGTAAAAC+AGG - chr5.2:33323329-33323348 MS.gene55360:intron 30.0%
! AACATTACCTTTGAAAGACT+TGG + chr5.2:33322819-33322838 None:intergenic 30.0%
! AACTTTTCAATAACCATGCA+TGG + chr5.2:33323157-33323176 None:intergenic 30.0%
! ATTTTCAGCAGAGAAGAAAA+AGG - chr5.2:33323289-33323308 MS.gene55360:intron 30.0%
! GTTTCTTTTCTTCTACAACT+TGG + chr5.2:33323678-33323697 None:intergenic 30.0%
! TGATAACGACTACATAGAAA+TGG - chr5.2:33323355-33323374 MS.gene55360:intron 30.0%
! TTTTCAGCAGAGAAGAAAAA+GGG - chr5.2:33323290-33323309 MS.gene55360:intron 30.0%
! TTTTTCTTCTCTGCTGAAAA+TGG + chr5.2:33323290-33323309 None:intergenic 30.0%
!!! GTTCACTCTGCAATTTTTTT+CGG + chr5.2:33324585-33324604 None:intergenic 30.0%
AAAAAGGCTTCATCTTTCAC+TGG - chr5.2:33323730-33323749 MS.gene55360:CDS 35.0%
AAAGGGAAGGTTGAAATCTT+TGG - chr5.2:33324507-33324526 MS.gene55360:intron 35.0%
AAAGTTACAAGCAAAAGGGA+AGG - chr5.2:33324717-33324736 MS.gene55360:intron 35.0%
AAATCTTCAAAGTCCCTTCT+CGG - chr5.2:33323497-33323516 MS.gene55360:intron 35.0%
AAATGAGAGAAGTGAAGGTA+TGG + chr5.2:33322857-33322876 None:intergenic 35.0%
AAGTCAGTTCAGAAAAACGT+GGG - chr5.2:33323227-33323246 MS.gene55360:intron 35.0%
AATTGCAGAGTGAACTTGAA+CGG - chr5.2:33324488-33324507 MS.gene55360:intron 35.0%
ACTACAACAGGATCAAACAA+TGG - chr5.2:33323856-33323875 MS.gene55360:CDS 35.0%
ACTTTGTCTGCATCATGTTT+AGG - chr5.2:33323631-33323650 MS.gene55360:CDS 35.0%
AGAAGAAAAGAAACCGGAAA+AGG - chr5.2:33323684-33323703 MS.gene55360:CDS 35.0%
AGTATATCCAAGCGATTATC+AGG - chr5.2:33323521-33323540 MS.gene55360:intron 35.0%
ATACCTTCACTTCTCTCATT+TGG - chr5.2:33322856-33322875 MS.gene55360:CDS 35.0%
ATTGCAGAGTGAACTTGAAA+AGG - chr5.2:33324489-33324508 MS.gene55360:intron 35.0%
CAGAAAACCAAGTCTTTCAA+AGG - chr5.2:33322809-33322828 MS.gene55360:CDS 35.0%
CTACAACAGGATCAAACAAT+GGG - chr5.2:33323857-33323876 MS.gene55360:CDS 35.0%
GATCGACAAGATCAAACAAT+GGG - chr5.2:33323912-33323931 MS.gene55360:intron 35.0%
GGTTGAAATGAGTACCTATT+GGG - chr5.2:33322935-33322954 MS.gene55360:CDS 35.0%
TACAACAGGATCAAACAATG+GGG - chr5.2:33323858-33323877 MS.gene55360:CDS 35.0%
TCTCTCAGACACTCTTTATT+AGG - chr5.2:33324192-33324211 MS.gene55360:CDS 35.0%
TGATCGACAAGATCAAACAA+TGG - chr5.2:33323911-33323930 MS.gene55360:intron 35.0%
TGGTTGAAATGAGTACCTAT+TGG - chr5.2:33322934-33322953 MS.gene55360:CDS 35.0%
TTCCTTGAAGTCTTCCTAAT+TGG + chr5.2:33323814-33323833 None:intergenic 35.0%
TTGCAGAGTGAACTTGAAAA+GGG - chr5.2:33324490-33324509 MS.gene55360:intron 35.0%
! AATGAGAGAAGTGAAGGTAT+GGG + chr5.2:33322856-33322875 None:intergenic 35.0%
! GTACCTTTGTTTGATCTTGT+CGG + chr5.2:33323962-33323981 None:intergenic 35.0%
! TACCTTTGTTTGATCTTGTC+GGG + chr5.2:33323961-33323980 None:intergenic 35.0%
!! CAAGATCAAACAAAGGTACT+TGG - chr5.2:33323963-33323982 MS.gene55360:intron 35.0%
!! GATAGAGTTGATAGATTCCT+GGG - chr5.2:33324119-33324138 MS.gene55360:CDS 35.0%
!!! CCTTTTTCATTGATAGTGCA+GGG + chr5.2:33323717-33323736 None:intergenic 35.0%
!!! CTTTTTCATTGATAGTGCAG+GGG + chr5.2:33323716-33323735 None:intergenic 35.0%
AGAGTGAACTTGAAAAGGGA+AGG - chr5.2:33324494-33324513 MS.gene55360:intron 40.0%
ATTGCAGAGTGAACTTGAAC+GGG - chr5.2:33324591-33324610 MS.gene55360:intron 40.0%
CAACTTATTATGTACCCCAC+TGG - chr5.2:33322907-33322926 MS.gene55360:CDS 40.0%
CAAGTCAGTTCAGAAAAACG+TGG - chr5.2:33323226-33323245 MS.gene55360:intron 40.0%
CACCAATTAGGAAGACTTCA+AGG - chr5.2:33323809-33323828 MS.gene55360:CDS 40.0%
CAGTGGGGTACATAATAAGT+TGG + chr5.2:33322909-33322928 None:intergenic 40.0%
CCCTGCACTATCAATGAAAA+AGG - chr5.2:33323714-33323733 MS.gene55360:CDS 40.0%
CTTGGATATACTACCGAGAA+GGG + chr5.2:33323513-33323532 None:intergenic 40.0%
GAAGTCTTCCTAATTGGTGA+TGG + chr5.2:33323808-33323827 None:intergenic 40.0%
GACAAATATCCAGGAGTTTG+AGG - chr5.2:33324811-33324830 MS.gene55360:CDS 40.0%
GACTACATAGAAATGGTGCA+TGG - chr5.2:33323362-33323381 MS.gene55360:intron 40.0%
TATCTTCTTCTTGTCTTCCC+AGG + chr5.2:33324139-33324158 None:intergenic 40.0%
TCTTCAATTAACTCCGCACT+TGG + chr5.2:33324754-33324773 None:intergenic 40.0%
TGCTAGACTTCATTCCCAAT+AGG + chr5.2:33322952-33322971 None:intergenic 40.0%
TTGTAGTCAGCTCTCTTTGA+GGG + chr5.2:33323843-33323862 None:intergenic 40.0%
! AAGTGAAGGTATGGGGAAAA+AGG + chr5.2:33322848-33322867 None:intergenic 40.0%
! ATGAGAGAAGTGAAGGTATG+GGG + chr5.2:33322855-33322874 None:intergenic 40.0%
! CACGTTTTTCTGAACTGACT+TGG + chr5.2:33323228-33323247 None:intergenic 40.0%
! CTCACACCACATATTTTACC+CGG + chr5.2:33324424-33324443 None:intergenic 40.0%
! GTCAAGACAGTTTTTACACG+AGG + chr5.2:33323268-33323287 None:intergenic 40.0%
!! AAAGACTTGGTTTTCTGCAC+AGG + chr5.2:33322806-33322825 None:intergenic 40.0%
!! CGATAGAGTTGATAGATTCC+TGG - chr5.2:33324118-33324137 MS.gene55360:CDS 40.0%
!! CTAATTGGTGATGGTAGTGA+CGG + chr5.2:33323799-33323818 None:intergenic 40.0%
!! TTGCTTCATTAGCTTGCGTT+TGG + chr5.2:33324166-33324185 None:intergenic 40.0%
!!! GCCTTTTTCATTGATAGTGC+AGG + chr5.2:33323718-33323737 None:intergenic 40.0%
!!! GCTGTTTTTGTAACCAACTC+TGG + chr5.2:33323316-33323335 None:intergenic 40.0%
ACGCAAGCTAATGAAGCAAC+AGG - chr5.2:33324167-33324186 MS.gene55360:CDS 45.0%
ACGGGGAAGGAAGAAATCTT+TGG - chr5.2:33324609-33324628 MS.gene55360:intron 45.0%
AGAAGAAAAAGGGCCAGAGT+TGG - chr5.2:33323300-33323319 MS.gene55360:intron 45.0%
AGCCCGACAAGATCAAACAA+AGG - chr5.2:33323956-33323975 MS.gene55360:intron 45.0%
ATTGGCCAAGGTCAAAAGCA+TGG - chr5.2:33323463-33323482 MS.gene55360:intron 45.0%
ATTTCAACCACTCTCCAGTG+GGG + chr5.2:33322924-33322943 None:intergenic 45.0%
CAAAGAGAGCTGACTACAAC+AGG - chr5.2:33323844-33323863 MS.gene55360:CDS 45.0%
CATTTCAACCACTCTCCAGT+GGG + chr5.2:33322925-33322944 None:intergenic 45.0%
CCACCAAATGAGAGAAGTGA+AGG + chr5.2:33322862-33322881 None:intergenic 45.0%
CCTTCACTTCTCTCATTTGG+TGG - chr5.2:33322859-33322878 MS.gene55360:CDS 45.0%
CGTCACTACCATCACCAATT+AGG - chr5.2:33323797-33323816 MS.gene55360:CDS 45.0%
GAGGAGAACAAAGCTGATCT+TGG - chr5.2:33322758-33322777 MS.gene55360:CDS 45.0%
GCTTGGATATACTACCGAGA+AGG + chr5.2:33323514-33323533 None:intergenic 45.0%
GGAGACAAGTGCAGATGAAT+GGG - chr5.2:33323879-33323898 MS.gene55360:CDS 45.0%
GGCAAGTACAATTCCAAGTG+CGG - chr5.2:33324738-33324757 MS.gene55360:intron 45.0%
GTTGTAGTCAGCTCTCTTTG+AGG + chr5.2:33323844-33323863 None:intergenic 45.0%
TAGAGGCCGGGTAAAATATG+TGG - chr5.2:33324415-33324434 MS.gene55360:intron 45.0%
TCATTTCAACCACTCTCCAG+TGG + chr5.2:33322926-33322945 None:intergenic 45.0%
TTGCAGAGTGAACTTGAACG+GGG - chr5.2:33324592-33324611 MS.gene55360:intron 45.0%
! AGGTATTTTCCGATCCCTTG+TGG + chr5.2:33322786-33322805 None:intergenic 45.0%
! TTCCACCATGCTTTTGACCT+TGG + chr5.2:33323471-33323490 None:intergenic 45.0%
!! CATTGATAGTGCAGGGGTAA+TGG + chr5.2:33323710-33323729 None:intergenic 45.0%
!! TTCTGAACTGACTTGGCTGT+TGG + chr5.2:33323221-33323240 None:intergenic 45.0%
AGAGTGAACTTGAACGGGGA+AGG - chr5.2:33324596-33324615 MS.gene55360:intron 50.0%
GGGAGACAAGTGCAGATGAA+TGG - chr5.2:33323878-33323897 MS.gene55360:CDS 50.0%
GGTACAACTTACTGGTACGG+AGG - chr5.2:33322739-33322758 MS.gene55360:CDS 50.0%
GTTCTCACTCTTCTGCTCAG+AGG + chr5.2:33323432-33323451 None:intergenic 50.0%
TGAGCAGAAGAGTGAGAACC+CGG - chr5.2:33323433-33323452 MS.gene55360:CDS 50.0%
TTATGTACCCCACTGGAGAG+TGG - chr5.2:33322914-33322933 MS.gene55360:CDS 50.0%
! GCTTTTGACCTTGGCCAATG+AGG + chr5.2:33323462-33323481 None:intergenic 50.0%
! TTTTCGGCTTTCCTCAGCCT+TGG + chr5.2:33324569-33324588 None:intergenic 50.0%
!! AAGCTGATCTTGGTGCCACA+AGG - chr5.2:33322768-33322787 MS.gene55360:CDS 50.0%
!! AGCTGATCTTGGTGCCACAA+GGG - chr5.2:33322769-33322788 MS.gene55360:CDS 50.0%
!! ATCTTGGTGCCACAAGGGAT+CGG - chr5.2:33322774-33322793 MS.gene55360:CDS 50.0%
AAGGCAGCTTTCCACAGCCT+TGG + chr5.2:33324689-33324708 None:intergenic 55.0%
TGCAGACAGAGTGAGAGCCA+AGG - chr5.2:33324669-33324688 MS.gene55360:intron 55.0%
! GATGGTAGTGACGGCAGTGA+AGG + chr5.2:33323790-33323809 None:intergenic 55.0%
! GGCAGTGAAGGAGGAGCTTT+TGG + chr5.2:33323778-33323797 None:intergenic 55.0%
! GGCCAAGGTCAAAAGCATGG+TGG - chr5.2:33323466-33323485 MS.gene55360:intron 55.0%
! GTCAGCTCTCTTTGAGGGTC+AGG + chr5.2:33323838-33323857 None:intergenic 55.0%
!! GCAGGGGTAATGGCCTTTTC+CGG + chr5.2:33323700-33323719 None:intergenic 55.0%
!!! AGGAGGAGCTTTTGGTGCTG+TGG + chr5.2:33323770-33323789 None:intergenic 55.0%
ACCTTGGCCAATGAGGCCTC+CGG + chr5.2:33323455-33323474 None:intergenic 60.0%
CAGAGTGAGAGCCAAGGCTG+TGG - chr5.2:33324675-33324694 MS.gene55360:intron 60.0%
GCAGAAGAGTGAGAACCCGG+AGG - chr5.2:33323436-33323455 MS.gene55360:CDS 60.0%
GGTAGTGACGGCAGTGAAGG+AGG + chr5.2:33323787-33323806 None:intergenic 60.0%
TGAGAACCCGGAGGCCTCAT+TGG - chr5.2:33323445-33323464 MS.gene55360:intron 60.0%
CCTTGGCCAATGAGGCCTCC+GGG + chr5.2:33323454-33323473 None:intergenic 65.0%
TGCAGACGGAGCGAGAGCCA+AGG - chr5.2:33324549-33324568 MS.gene55360:intron 65.0%
CCCGGAGGCCTCATTGGCCA+AGG - chr5.2:33323451-33323470 MS.gene55360:intron 70.0%
CGGAGCGAGAGCCAAGGCTG+AGG - chr5.2:33324555-33324574 MS.gene55360:intron 70.0%


Chromosome Type Strat End Strand Name
chr5.2 gene 33322739 33324831 33322739 ID=MS.gene55360
chr5.2 mRNA 33322739 33324831 33322739 ID=MS.gene55360.t1;Parent=MS.gene55360
chr5.2 exon 33324740 33324831 33324740 ID=MS.gene55360.t1.exon1;Parent=MS.gene55360.t1
chr5.2 CDS 33324740 33324831 33324740 ID=cds.MS.gene55360.t1;Parent=MS.gene55360.t1
chr5.2 exon 33324028 33324270 33324028 ID=MS.gene55360.t1.exon2;Parent=MS.gene55360.t1
chr5.2 CDS 33324028 33324270 33324028 ID=cds.MS.gene55360.t1;Parent=MS.gene55360.t1
chr5.2 exon 33323593 33323917 33323593 ID=MS.gene55360.t1.exon3;Parent=MS.gene55360.t1
chr5.2 CDS 33323593 33323917 33323593 ID=cds.MS.gene55360.t1;Parent=MS.gene55360.t1
chr5.2 exon 33323382 33323463 33323382 ID=MS.gene55360.t1.exon4;Parent=MS.gene55360.t1
chr5.2 CDS 33323382 33323463 33323382 ID=cds.MS.gene55360.t1;Parent=MS.gene55360.t1
chr5.2 exon 33322739 33323074 33322739 ID=MS.gene55360.t1.exon5;Parent=MS.gene55360.t1
chr5.2 CDS 33322739 33323074 33322739 ID=cds.MS.gene55360.t1;Parent=MS.gene55360.t1
Gene Sequence

>MS.gene55360

GGTACAACTTACTGGTACGGAGGAGAACAAAGCTGATCTTGGTGCCACAAGGGATCGGAAAATACCTGTGCAGAAAACCAAGTCTTTCAAAGGTAATGTTTTGTTACCTTTTTCCCCATACCTTCACTTCTCTCATTTGGTGGTTTCCGAACCAATATATAATATATCCAACTTATTATGTACCCCACTGGAGAGTGGTTGAAATGAGTACCTATTGGGAATGAAGTCTAGCACAGCTAATCTCTCGTTCACTTCTCTCTTGTAGTGTATGCTTAGTGTTTGTTTGATAACTAGATGTAGAACTTACCATAAAAATGAGTCTATTTCATAATTTTACTAATTTGTCAAGCATTTACCATAAAAATGAGGAAAAGAAACAGAAAGAAACAAAGATAAAAAAAAAAAAAAAATTATGCCATGCATGGTTATTGAAAAGTTTTAGAAATGAAGACTAGACAATTACACATAAGAGCATGCAACCAACAGCCAAGTCAGTTCAGAAAAACGTGGGATAATTTTCATTTGACCTCGTGTAAAAACTGTCTTGACCATTTTCAGCAGAGAAGAAAAAGGGCCAGAGTTGGTTACAAAAACAGCTTTCAAGTAAAACAGGTCGTGATAACGACTACATAGAAATGGTGCATGGTGCAGCAGTAGCTGCTGCTGCATTCTCAATTAATTTGCAAGAGACCTCTGAGCAGAAGAGTGAGAACCCGGAGGCCTCATTGGCCAAGGTCAAAAGCATGGTGGAAAGCTCAAAATCTTCAAAGTCCCTTCTCGGTAGTATATCCAAGCGATTATCAGGTAAATATGAGTACATACCACTCCATTTATGAATCATAATTACCATTACTCTTAAGTAAAAAAACAGAAAGAAAATCAAGTTTATTAAACTTTGTCTGCATCATGTTTAGGTTCATTTAGATCTAAAGATGACCAAGTTGTAGAAGAAAAGAAACCGGAAAAGGCCATTACCCCTGCACTATCAATGAAAAAGGCTTCATCTTTCACTGGAAAAAAAAATGACACCACAGCACCAAAAGCTCCTCCTTCACTGCCGTCACTACCATCACCAATTAGGAAGACTTCAAGGAAACCTGACCCTCAAAGAGAGCTGACTACAACAGGATCAAACAATGGGGAGACAAGTGCAGATGAATGGGAGAGAACTGTGATCGACAAGATCAAACAATGGGCAAATAAACAGCAGAGAACTGAGCCCGACAAGATCAAACAAAGGTACTTGGAAGCATGATTACGTTGAATTTCTAACAAAGAAAACACTCTCAAACATAATTACAATAAATATTATCATCTCTTGAATCAAGCATTACATGCAGTTTATATACTAATGAAAAACATTGTCAGGTATGAGAAGTCGATAGAGTTGATAGATTCCTGGGAAGACAAGAAGAAGATAAAAGCCAAACGCAAGCTAATGAAGCAACAGGTTTCTCTCAGACACTCTTTATTAGGTTCCTTAGATCTTATTCATTTAGTGCTTTCTTTAAGCAGGTCATGAAATGCTTCCTTACCTATAATCAGATAATGTTCTAGGTCTTGTTAAATTTGTGTCTTATTCATAACTTTTTCAGAGTTCAAAACATGTGAGTAGATGCTATCTGTAAAACAATTAGCAGTCTAGTATGAACAATAAGTCTGGAATGATAAAAAAATAGAGGCCGGGTAAAATATGTGGTGTGAGATGAAAAGTTGAACTTTAATTTAAAAACTAATTGCAAACTTCTAAATTGCAGAGTGAACTTGAAAAGGGAAGGTTGAAATCTTTGGAGAAGTATGTAAATCTAATTGCAGACGGAGCGAGAGCCAAGGCTGAGGAAAGCCGAAAAAAATTGCAGAGTGAACTTGAACGGGGAAGGAAGAAATCTTTGGAGAAATTTCAAAATAAGATGACGTATGTAAATCTAGTTGCAGACAGAGTGAGAGCCAAGGCTGTGGAAAGCTGCCTTAAAAATGAAAAGTTACAAGCAAAAGGGAAGGCAAGTACAATTCCAAGTGCGGAGTTAATTGAAGAGTTAATTGACAAATATCCGGCCATTTACAAAAAAATTGACAAATATCCAGGAGTTTGA
Protein sequence

>MS.gene55360.t1

GTTYWYGGEQSUSWCHKGSENTCAENQVFQREEKGPELVTKTAFKUNRSUURLHRNGAWCSSSCCCILNUFARDLUAEEUEPGGLIGQGQKHGGKLKIFKVPSRUYIQAIIRFIUIURUPSCRRKETGKGHYPCTINEKGFIFHWKKKUHHSTKSSSFTAVTTITNUEDFKETUPSKRADYNRIKQWGDKCRUMGENCDRQDQTMGKUTAENUARQDQTKVUEVDRVDRFLGRQEEDKSQTQANEATEUTUKGKVEIFGEVCKSNCRRSESQGUGKPKKIAEUTUTGKEEIFGEISKUDDVCKSSCRQSESQGCGKLPUKUKVTSKREGKYNSKCGVNURVNUQISGHLQKNUQISRSL