Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene55575.t1 | XP_003618944.2 | 93.4 | 542 | 36 | 0 | 1 | 542 | 1 | 542 | 1.20E-216 | 762.7 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene55575.t1 | Q9NQG5 | 42.2 | 116 | 66 | 1 | 5 | 119 | 3 | 118 | 1.3e-19 | 99.4 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene55575.t1 | G7KPK5 | 93.4 | 542 | 36 | 0 | 1 | 542 | 1 | 542 | 8.4e-217 | 762.7 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene057451 | MS.gene55575 | 0.803677 | 3.11E-49 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene55575.t1 | MTR_6g027180 | 96.310 | 542 | 20 | 0 | 1 | 542 | 1 | 542 | 0.0 | 1062 |
| MS.gene55575.t1 | MTR_7g087450 | 63.230 | 514 | 145 | 8 | 27 | 529 | 9 | 489 | 0.0 | 548 |
| MS.gene55575.t1 | MTR_7g087450 | 60.902 | 399 | 120 | 6 | 134 | 529 | 12 | 377 | 1.47e-127 | 378 |
| MS.gene55575.t1 | MTR_7g026740 | 39.753 | 405 | 215 | 7 | 1 | 386 | 1 | 395 | 1.30e-76 | 251 |
| MS.gene55575.t1 | MTR_3g087840 | 36.939 | 490 | 231 | 10 | 10 | 472 | 11 | 449 | 2.35e-76 | 251 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene55575.t1 | AT3G26990 | 42.446 | 417 | 189 | 13 | 1 | 386 | 1 | 397 | 4.40e-77 | 252 |
| MS.gene55575.t1 | AT3G26990 | 42.446 | 417 | 189 | 13 | 1 | 386 | 1 | 397 | 4.40e-77 | 252 |
| MS.gene55575.t1 | AT5G65180 | 37.562 | 402 | 207 | 9 | 6 | 390 | 5 | 379 | 3.23e-75 | 245 |
| MS.gene55575.t1 | AT5G10060 | 38.575 | 407 | 198 | 8 | 5 | 397 | 4 | 372 | 9.27e-71 | 234 |
| MS.gene55575.t1 | AT5G65180 | 31.395 | 258 | 141 | 7 | 142 | 390 | 21 | 251 | 1.13e-25 | 107 |
Find 134 sgRNAs with CRISPR-Local
Find 0 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| AAGGGACACGCTGCTCTTTC+TGG | 0.128362 | 6.2:-18410485 | None:intergenic |
| TCGGGCACCCTTGTAGTTTC+TGG | 0.175172 | 6.2:-18417505 | None:intergenic |
| TCTCTCTTAGCTATCTTTAT+AGG | 0.198808 | 6.2:-18412649 | None:intergenic |
| GGATTGTGATGGCTGATTAC+TGG | 0.206833 | 6.2:-18418136 | None:intergenic |
| TTGAAAATGCTGAGGCTATC+AGG | 0.230933 | 6.2:+18414817 | MS.gene55575:CDS |
| GTGAGTTCGTTAATGAATTC+TGG | 0.236271 | 6.2:+18410556 | MS.gene55575:CDS |
| TAATTGATTTGTTTGTGATA+TGG | 0.269583 | 6.2:-18417929 | None:intergenic |
| AGGCATTTGCTGTTCAAGTT+TGG | 0.291640 | 6.2:-18417803 | None:intergenic |
| GGCATTTGCTGTTCAAGTTT+GGG | 0.318843 | 6.2:-18417802 | None:intergenic |
| CCAGCTTAAAGAAGCATTGC+AGG | 0.323110 | 6.2:+18414854 | MS.gene55575:CDS |
| CCCTTGTAGTTTCTGGTGCT+TGG | 0.323387 | 6.2:-18417498 | None:intergenic |
| AGAGTTCAAACTACCATTCT+TGG | 0.335914 | 6.2:-18417734 | None:intergenic |
| CAGCTGCTGCTGTTGCTGCT+AGG | 0.343943 | 6.2:+18417638 | MS.gene55575:CDS |
| TTGCCTCCACATGTGGCAAT+GGG | 0.344380 | 6.2:+18418102 | MS.gene55575:CDS |
| TTGAGCAAGTAGCCAGTATC+AGG | 0.351819 | 6.2:+18417464 | MS.gene55575:CDS |
| GTCGGTGCCGGTGGTGTTGA+TGG | 0.363221 | 6.2:-18418249 | None:intergenic |
| TGAGTAAAAGAAGGCGGAGG+AGG | 0.370830 | 6.2:-18417562 | None:intergenic |
| GGCGGAGGAGGGGTGCTGTT+TGG | 0.380413 | 6.2:-18417550 | None:intergenic |
| GAACTCTGCTGAGTTTAATT+CGG | 0.384193 | 6.2:+18417750 | MS.gene55575:CDS |
| TTTGCCTCCACATGTGGCAA+TGG | 0.390143 | 6.2:+18418101 | MS.gene55575:CDS |
| GGAGGCAAAGGAGGTGGAGG+TGG | 0.393872 | 6.2:-18418087 | None:intergenic |
| GCTAAACCTCTTCCTGATAC+TGG | 0.394101 | 6.2:-18417476 | None:intergenic |
| TCATTGCAGAAACTAGCTAT+TGG | 0.402677 | 6.2:+18412792 | MS.gene55575:intron |
| GGCAAAGGAGGTGGAGGTGG+AGG | 0.404017 | 6.2:-18418084 | None:intergenic |
| ACAGATGGAGTAGTTTGTTC+GGG | 0.405377 | 6.2:-18417523 | None:intergenic |
| AGGGACACGCTGCTCTTTCT+GGG | 0.408135 | 6.2:-18410484 | None:intergenic |
| TACTGGCTACTTGCTCAATA+TGG | 0.410101 | 6.2:-18417459 | None:intergenic |
| AATCTGCCGGAATGATGGTT+GGG | 0.411020 | 6.2:+18418025 | MS.gene55575:CDS |
| GATTGTGATGGCTGATTACT+GGG | 0.411854 | 6.2:-18418135 | None:intergenic |
| AACAGATGGAGTAGTTTGTT+CGG | 0.419301 | 6.2:-18417524 | None:intergenic |
| CTGTTGTTTAGATTGACATA+TGG | 0.421715 | 6.2:+18412545 | MS.gene55575:intron |
| CCTGCAATGCTTCTTTAAGC+TGG | 0.425948 | 6.2:-18414854 | None:intergenic |
| GAGGTGGAGGTGGAGGTTGT+AGG | 0.428218 | 6.2:-18418077 | None:intergenic |
| GATTGCATGCTTTGTGGTGT+TGG | 0.432680 | 6.2:-18417907 | None:intergenic |
| TGTTGTTTAGATTGACATAT+GGG | 0.436662 | 6.2:+18412546 | MS.gene55575:intron |
| TGACATATGGGAGGAAAGAA+AGG | 0.438296 | 6.2:+18412558 | MS.gene55575:CDS |
| GAGTAAAAGAAGGCGGAGGA+GGG | 0.438801 | 6.2:-18417561 | None:intergenic |
| GCTTGCTGAGCCTGTGATTG+AGG | 0.441371 | 6.2:-18418159 | None:intergenic |
| CTTGCTGAGCCTGTGATTGA+GGG | 0.445266 | 6.2:-18418158 | None:intergenic |
| TCCGGTGAGAAACACACAAT+CGG | 0.453289 | 6.2:-18410413 | None:intergenic |
| TAAAGAAGCATTGCAGGAAC+AGG | 0.456641 | 6.2:+18414860 | MS.gene55575:CDS |
| TCATTAGCCAAGTTCAGAAA+AGG | 0.456784 | 6.2:-18410504 | None:intergenic |
| ATGACATCTTGCAAAATAGT+AGG | 0.462528 | 6.2:+18410523 | MS.gene55575:CDS |
| ATGTGGCAATGGGGTTAACT+AGG | 0.465467 | 6.2:+18418112 | MS.gene55575:CDS |
| AGCAGCATTACACTTGTTCA+AGG | 0.472092 | 6.2:-18412883 | None:intergenic |
| CAGCAACAACAATCGTCAAC+TGG | 0.473378 | 6.2:+18418186 | MS.gene55575:CDS |
| CTGCAATGCTTCTTTAAGCT+GGG | 0.473713 | 6.2:-18414853 | None:intergenic |
| GAAACATGGAATAAATCGTT+CGG | 0.474595 | 6.2:+18410456 | MS.gene55575:CDS |
| CGTTCAATCTGCCGGAATGA+TGG | 0.478975 | 6.2:+18418020 | MS.gene55575:CDS |
| GATATGGATTGCATGCTTTG+TGG | 0.485287 | 6.2:-18417913 | None:intergenic |
| GGTCTAAAGGATGAAATAAC+GGG | 0.488528 | 6.2:+18412601 | MS.gene55575:CDS |
| TGCATGCTTTGTGGTGTTGG+TGG | 0.489605 | 6.2:-18417904 | None:intergenic |
| GTGCCGGTGGTGTTGATGGA+TGG | 0.491994 | 6.2:-18418245 | None:intergenic |
| CAATCTGCCGGAATGATGGT+TGG | 0.492596 | 6.2:+18418024 | MS.gene55575:CDS |
| GAACTGTTGACATCAGAAAC+AGG | 0.493387 | 6.2:-18417823 | None:intergenic |
| ACCTCCTTTGCCTCCACATG+TGG | 0.498400 | 6.2:+18418095 | MS.gene55575:CDS |
| TTGTTTGATCCATATCCATA+AGG | 0.498767 | 6.2:-18418054 | None:intergenic |
| TGACTTGCAACAGCAGGAAA+AGG | 0.503422 | 6.2:+18414770 | MS.gene55575:CDS |
| AGGTCTAAAGGATGAAATAA+CGG | 0.505301 | 6.2:+18412600 | MS.gene55575:CDS |
| AACTACTGAAGATGATAAGA+AGG | 0.505508 | 6.2:+18417612 | MS.gene55575:CDS |
| ACATGTGGAGGCAAAGGAGG+TGG | 0.510516 | 6.2:-18418093 | None:intergenic |
| CCCGATTGTGTGTTTCTCAC+CGG | 0.512217 | 6.2:+18410412 | MS.gene55575:CDS |
| GGTTAATGACTTGCAACAGC+AGG | 0.512456 | 6.2:+18414764 | MS.gene55575:CDS |
| CACTGTCTAGGTGTCGGTGC+CGG | 0.512485 | 6.2:-18418261 | None:intergenic |
| CGAAGAAGGTTGTTGAAAAG+TGG | 0.512623 | 6.2:-18417872 | None:intergenic |
| GAGTTTAATTCGGCACCACC+TGG | 0.514130 | 6.2:+18417760 | MS.gene55575:CDS |
| GATGTCAACAGTTCTGATAC+CGG | 0.516824 | 6.2:+18417832 | MS.gene55575:CDS |
| TGTTTATGCAAGTGATGAGT+CGG | 0.517072 | 6.2:+18410605 | MS.gene55575:CDS |
| GGTGGCATATTTGCCGAAGA+AGG | 0.520474 | 6.2:-18417886 | None:intergenic |
| TAAACACGCTTGAGCGCTGA+TGG | 0.526566 | 6.2:-18410588 | None:intergenic |
| ATCACAATCCCCTCAATCAC+AGG | 0.527639 | 6.2:+18418149 | MS.gene55575:CDS |
| GTGCTGTTTGGTTGAACAGA+TGG | 0.528038 | 6.2:-18417538 | None:intergenic |
| GCCAAGCACCAGAAACTACA+AGG | 0.528803 | 6.2:+18417497 | MS.gene55575:CDS |
| GAAGAAGCTGCCTCCAAGAA+TGG | 0.530265 | 6.2:+18417721 | MS.gene55575:CDS |
| TGAAAATGCTGAGGCTATCA+GGG | 0.534674 | 6.2:+18414818 | MS.gene55575:CDS |
| ATGGGAAACGATGCATATGA+TGG | 0.535957 | 6.2:+18409267 | MS.gene55575:CDS |
| ATCTGCCGGAATGATGGTTG+GGG | 0.536972 | 6.2:+18418026 | MS.gene55575:CDS |
| GTTCAAACTACCATTCTTGG+AGG | 0.537055 | 6.2:-18417731 | None:intergenic |
| TGTGGAGGCAAAGGAGGTGG+AGG | 0.538614 | 6.2:-18418090 | None:intergenic |
| TTCATATGTGAATGAGGAGG+GGG | 0.540904 | 6.2:-18417979 | None:intergenic |
| GGTTGAGTAAAAGAAGGCGG+AGG | 0.546883 | 6.2:-18417565 | None:intergenic |
| CCGGTGAGAAACACACAATC+GGG | 0.547672 | 6.2:-18410412 | None:intergenic |
| AATCAGTACGTTCAATCTGC+CGG | 0.549534 | 6.2:+18418012 | MS.gene55575:CDS |
| AAGTAGCCAGTATCAGGAAG+AGG | 0.551585 | 6.2:+18417470 | MS.gene55575:CDS |
| TCATCTTCAGTAGTTTGAAG+AGG | 0.553774 | 6.2:-18417604 | None:intergenic |
| TTCGCGGTCTCAAGGTCTAA+AGG | 0.554342 | 6.2:+18412588 | MS.gene55575:CDS |
| CATTAGCCAAGTTCAGAAAA+GGG | 0.555475 | 6.2:-18410503 | None:intergenic |
| GAAATAACGGGCAAGAGCAA+TGG | 0.557957 | 6.2:+18412613 | MS.gene55575:CDS |
| GATTCATATGTGAATGAGGA+GGG | 0.561300 | 6.2:-18417981 | None:intergenic |
| TCATTAACCAAGGTGGATCC+CGG | 0.564911 | 6.2:-18414750 | None:intergenic |
| TGTCTAGGTGTCGGTGCCGG+TGG | 0.566815 | 6.2:-18418258 | None:intergenic |
| AGGCAGCTTCTTCAGCAACA+AGG | 0.566859 | 6.2:-18417711 | None:intergenic |
| GTTTATGCAAGTGATGAGTC+GGG | 0.568200 | 6.2:+18410606 | MS.gene55575:CDS |
| TTAAACAATACTCACTGTCT+AGG | 0.568370 | 6.2:-18418273 | None:intergenic |
| TGAACGTACTGATTTGCTTG+TGG | 0.571604 | 6.2:-18418003 | None:intergenic |
| AAATCAACCGGGATCCACCT+TGG | 0.572720 | 6.2:+18414743 | MS.gene55575:CDS |
| AACCCCATTGCCACATGTGG+AGG | 0.573744 | 6.2:-18418105 | None:intergenic |
| CAAAAGATATAGTTGAAACA+TGG | 0.573745 | 6.2:+18410442 | MS.gene55575:CDS |
| ATTGCCACATGTGGAGGCAA+AGG | 0.576539 | 6.2:-18418099 | None:intergenic |
| GGCAGCTTCTTCAGCAACAA+GGG | 0.577697 | 6.2:-18417710 | None:intergenic |
| GCCACATGTGGAGGCAAAGG+AGG | 0.583577 | 6.2:-18418096 | None:intergenic |
| ATGGTAGGTTGAGTAAAAGA+AGG | 0.588830 | 6.2:-18417571 | None:intergenic |
| TTGCTGAGCCTGTGATTGAG+GGG | 0.590248 | 6.2:-18418157 | None:intergenic |
| GTTGAAAATATACTCTCTCA+AGG | 0.590706 | 6.2:+18412936 | MS.gene55575:CDS |
| TCACAAACAAATCAATTACA+AGG | 0.591572 | 6.2:+18417934 | MS.gene55575:CDS |
| AGTAAAAGAAGGCGGAGGAG+GGG | 0.600428 | 6.2:-18417560 | None:intergenic |
| GTTAACCCCATTGCCACATG+TGG | 0.600644 | 6.2:-18418108 | None:intergenic |
| TAATGCTGCTGTGTCTCAAG+TGG | 0.600836 | 6.2:+18412896 | MS.gene55575:CDS |
| TTGCAAAATAGTAGGCGGAA+AGG | 0.603658 | 6.2:+18410531 | MS.gene55575:CDS |
| TGCCTCCACATGTGGCAATG+GGG | 0.617585 | 6.2:+18418103 | MS.gene55575:CDS |
| ATGTCAACAGTTCTGATACC+GGG | 0.617988 | 6.2:+18417833 | MS.gene55575:CDS |
| AATCCATCCATCAACACCAC+CGG | 0.625216 | 6.2:+18418242 | MS.gene55575:CDS |
| TGTGATTGAGGGGATTGTGA+TGG | 0.629108 | 6.2:-18418147 | None:intergenic |
| AGAGGAGCAAATGACATGGT+AGG | 0.637452 | 6.2:-18417586 | None:intergenic |
| TGTTTAGATTGACATATGGG+AGG | 0.637883 | 6.2:+18412549 | MS.gene55575:intron |
| TGTGGATTCATATGTGAATG+AGG | 0.639619 | 6.2:-18417985 | None:intergenic |
| ATTCATATGTGAATGAGGAG+GGG | 0.643039 | 6.2:-18417980 | None:intergenic |
| TCTGCCGGAATGATGGTTGG+GGG | 0.651928 | 6.2:+18418027 | MS.gene55575:CDS |
| AGTGCAACTTGAAAATGCTG+AGG | 0.655360 | 6.2:+18414809 | MS.gene55575:CDS |
| AATACTCACTGTCTAGGTGT+CGG | 0.656627 | 6.2:-18418267 | None:intergenic |
| AATGCTGCTGTGTCTCAAGT+GGG | 0.657611 | 6.2:+18412897 | MS.gene55575:CDS |
| ACATCTTGCAAAATAGTAGG+CGG | 0.662156 | 6.2:+18410526 | MS.gene55575:CDS |
| TTCTCAGGATGGAAACCAGG+TGG | 0.665372 | 6.2:-18417775 | None:intergenic |
| TGGATTTGCCCATAAAACCC+AGG | 0.665888 | 6.2:-18418225 | None:intergenic |
| TTGCAAGTCATTAACCAAGG+TGG | 0.676669 | 6.2:-18414757 | None:intergenic |
| CAACAACAATCGTCAACTGG+AGG | 0.677051 | 6.2:+18418189 | MS.gene55575:CDS |
| CTGTTGCAAGTCATTAACCA+AGG | 0.692415 | 6.2:-18414760 | None:intergenic |
| AGATGTGAGCATGAGAGCTG+AGG | 0.698052 | 6.2:-18417677 | None:intergenic |
| GGATTCATATGTGAATGAGG+AGG | 0.699534 | 6.2:-18417982 | None:intergenic |
| TTGAAGAGGAGCAAATGACA+TGG | 0.701085 | 6.2:-18417590 | None:intergenic |
| GTAGGTTGAGTAAAAGAAGG+CGG | 0.708710 | 6.2:-18417568 | None:intergenic |
| AAATGAAGAACATTCTAACG+AGG | 0.730778 | 6.2:+18412854 | MS.gene55575:CDS |
| CCAAGCACCAGAAACTACAA+GGG | 0.731251 | 6.2:+18417498 | MS.gene55575:CDS |
| ATTTGCCCATAAAACCCAGG+TGG | 0.756034 | 6.2:-18418222 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr6.2 | gene | 18409267 | 18418284 | 18409267 | ID=MS.gene55575 |
| chr6.2 | mRNA | 18409267 | 18418284 | 18409267 | ID=MS.gene55575.t1;Parent=MS.gene55575 |
| chr6.2 | exon | 18409267 | 18409345 | 18409267 | ID=MS.gene55575.t1.exon1;Parent=MS.gene55575.t1 |
| chr6.2 | CDS | 18409267 | 18409345 | 18409267 | ID=cds.MS.gene55575.t1;Parent=MS.gene55575.t1 |
| chr6.2 | exon | 18410406 | 18410650 | 18410406 | ID=MS.gene55575.t1.exon2;Parent=MS.gene55575.t1 |
| chr6.2 | CDS | 18410406 | 18410650 | 18410406 | ID=cds.MS.gene55575.t1;Parent=MS.gene55575.t1 |
| chr6.2 | exon | 18412556 | 18412690 | 18412556 | ID=MS.gene55575.t1.exon3;Parent=MS.gene55575.t1 |
| chr6.2 | CDS | 18412556 | 18412690 | 18412556 | ID=cds.MS.gene55575.t1;Parent=MS.gene55575.t1 |
| chr6.2 | exon | 18412801 | 18412957 | 18412801 | ID=MS.gene55575.t1.exon4;Parent=MS.gene55575.t1 |
| chr6.2 | CDS | 18412801 | 18412957 | 18412801 | ID=cds.MS.gene55575.t1;Parent=MS.gene55575.t1 |
| chr6.2 | exon | 18414742 | 18414881 | 18414742 | ID=MS.gene55575.t1.exon5;Parent=MS.gene55575.t1 |
| chr6.2 | CDS | 18414742 | 18414881 | 18414742 | ID=cds.MS.gene55575.t1;Parent=MS.gene55575.t1 |
| chr6.2 | exon | 18414956 | 18414991 | 18414956 | ID=MS.gene55575.t1.exon6;Parent=MS.gene55575.t1 |
| chr6.2 | CDS | 18414956 | 18414991 | 18414956 | ID=cds.MS.gene55575.t1;Parent=MS.gene55575.t1 |
| chr6.2 | exon | 18417448 | 18418284 | 18417448 | ID=MS.gene55575.t1.exon7;Parent=MS.gene55575.t1 |
| chr6.2 | CDS | 18417448 | 18418284 | 18417448 | ID=cds.MS.gene55575.t1;Parent=MS.gene55575.t1 |
| Gene Sequence |
| Protein sequence |
