Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene55680.t1 | KEH34139.1 | 43.5 | 124 | 41 | 3 | 1 | 100 | 1 | 119 | 2.20E-12 | 81.6 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene55680.t1 | A0A072UWC0 | 43.5 | 124 | 41 | 3 | 1 | 100 | 1 | 119 | 1.6e-12 | 81.6 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene049631 | MS.gene55680 | 0.878376 | 2.95E-69 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene55680.t1 | MTR_3g462080 | 44.355 | 124 | 40 | 4 | 1 | 100 | 1 | 119 | 4.20e-22 | 83.6 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Find 27 sgRNAs with CRISPR-Local
Find 39 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| AGGAGATGTTTACTCGTTAT+TGG | 0.269194 | 3.3:+57137187 | MS.gene55680:CDS |
| TACTACCCTCCATACCCTTC+TGG | 0.348454 | 3.3:-57137212 | None:intergenic |
| CGAGCCTCCGTTTCTGCATC+CGG | 0.409336 | 3.3:+57137324 | MS.gene55680:CDS |
| GGATGCAGAAACGGAGGCTC+GGG | 0.468227 | 3.3:-57137322 | None:intergenic |
| AACATGTCCGGATGCAGAAA+CGG | 0.483189 | 3.3:-57137331 | None:intergenic |
| AGCAGCAGCAGCAACATGTC+CGG | 0.500108 | 3.3:-57137343 | None:intergenic |
| CGGATGCAGAAACGGAGGCT+CGG | 0.503641 | 3.3:-57137323 | None:intergenic |
| TACTCGTTATTGGTTCCAGA+AGG | 0.510115 | 3.3:+57137197 | MS.gene55680:CDS |
| GACACCACGAACTCCATCCT+TGG | 0.528708 | 3.3:-57137082 | None:intergenic |
| TTATTGGTTCCAGAAGGGTA+TGG | 0.546823 | 3.3:+57137203 | MS.gene55680:CDS |
| GGAGTTCGTGGTGTCGAGGA+AGG | 0.547698 | 3.3:+57137090 | MS.gene55680:CDS |
| GGGACGTCAAGAATGGAGAG+GGG | 0.556376 | 3.3:+57137145 | MS.gene55680:CDS |
| GATACCAAGGATGGAGTTCG+TGG | 0.560422 | 3.3:+57137078 | MS.gene55680:CDS |
| GGATGGACTCAAGGTTGCAC+AGG | 0.574542 | 3.3:+57137167 | MS.gene55680:CDS |
| GCTGTTGATTGTTTCAACCA+AGG | 0.576773 | 3.3:+57137291 | MS.gene55680:CDS |
| GGGGACGTCAAGAATGGAGA+GGG | 0.581994 | 3.3:+57137144 | MS.gene55680:CDS |
| GGACGTCAAGAATGGAGAGG+GGG | 0.582490 | 3.3:+57137146 | MS.gene55680:CDS |
| TGCAAAAGGAATGGATACCA+AGG | 0.584177 | 3.3:+57137065 | None:intergenic |
| AGGCTCGGGAAGCAGAACCT+TGG | 0.590344 | 3.3:-57137308 | None:intergenic |
| AAAGGAATGGATACCAAGGA+TGG | 0.599291 | 3.3:+57137069 | None:intergenic |
| TGGTTCCAGAAGGGTATGGA+GGG | 0.601975 | 3.3:+57137207 | MS.gene55680:CDS |
| GGATGGAGTTCGTGGTGTCG+AGG | 0.604594 | 3.3:+57137086 | MS.gene55680:CDS |
| TTGGTTCCAGAAGGGTATGG+AGG | 0.608678 | 3.3:+57137206 | MS.gene55680:CDS |
| AACATAGTCTACTCCTCACT+CGG | 0.615857 | 3.3:-57137373 | None:intergenic |
| ACTCGTTATTGGTTCCAGAA+GGG | 0.618430 | 3.3:+57137198 | MS.gene55680:CDS |
| GCTGCTACTGATACCGAGTG+AGG | 0.682392 | 3.3:+57137360 | MS.gene55680:CDS |
| ATGTCCGGATGCAGAAACGG+AGG | 0.716074 | 3.3:-57137328 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| AGGAGATGTTTACTCGTTAT+TGG | + | chr3.3:57137187-57137206 | MS.gene55680:CDS | 35.0% | |
| !! | AAGTCCAGCTTTTTGAAAGA+TGG | - | chr3.3:57137253-57137272 | None:intergenic | 35.0% |
| ! | AGTTCGTGATCTCGTTTTAG+GGG | + | chr3.3:57137113-57137132 | MS.gene55680:CDS | 40.0% |
| ! | GAGTTCGTGATCTCGTTTTA+GGG | + | chr3.3:57137112-57137131 | MS.gene55680:CDS | 40.0% |
| ! | GCTGTTGATTGTTTCAACCA+AGG | + | chr3.3:57137291-57137310 | MS.gene55680:CDS | 40.0% |
| !! | ACTCGTTATTGGTTCCAGAA+GGG | + | chr3.3:57137198-57137217 | MS.gene55680:CDS | 40.0% |
| !! | TACTCGTTATTGGTTCCAGA+AGG | + | chr3.3:57137197-57137216 | MS.gene55680:CDS | 40.0% |
| !! | TTATTGGTTCCAGAAGGGTA+TGG | + | chr3.3:57137203-57137222 | MS.gene55680:CDS | 40.0% |
| !! | TTTTGAAAGATGGCAGGTGA+TGG | - | chr3.3:57137243-57137262 | None:intergenic | 40.0% |
| AACATGTCCGGATGCAGAAA+CGG | - | chr3.3:57137334-57137353 | None:intergenic | 45.0% | |
| CCTGCCATCTTTCAAAAAGC+TGG | + | chr3.3:57137246-57137265 | MS.gene55680:CDS | 45.0% | |
| ! | GGAGTTCGTGATCTCGTTTT+AGG | + | chr3.3:57137111-57137130 | MS.gene55680:CDS | 45.0% |
| !! | CCAGCTTTTTGAAAGATGGC+AGG | - | chr3.3:57137249-57137268 | None:intergenic | 45.0% |
| GATACCAAGGATGGAGTTCG+TGG | + | chr3.3:57137078-57137097 | MS.gene55680:CDS | 50.0% | |
| TACTACCCTCCATACCCTTC+TGG | - | chr3.3:57137215-57137234 | None:intergenic | 50.0% | |
| ! | TGGTTCCAGAAGGGTATGGA+GGG | + | chr3.3:57137207-57137226 | MS.gene55680:CDS | 50.0% |
| ! | TTGGTTCCAGAAGGGTATGG+AGG | + | chr3.3:57137206-57137225 | MS.gene55680:CDS | 50.0% |
| !!! | CTCGTTTTAGGGGTGGTAGA+GGG | + | chr3.3:57137123-57137142 | MS.gene55680:CDS | 50.0% |
| !!! | TCGTGATCTCGTTTTAGGGG+TGG | + | chr3.3:57137116-57137135 | MS.gene55680:CDS | 50.0% |
| !!! | TCGTTTTAGGGGTGGTAGAG+GGG | + | chr3.3:57137124-57137143 | MS.gene55680:CDS | 50.0% |
| !!! | TCTCGTTTTAGGGGTGGTAG+AGG | + | chr3.3:57137122-57137141 | MS.gene55680:CDS | 50.0% |
| AGCAGCAGCAGCAACATGTC+CGG | - | chr3.3:57137346-57137365 | None:intergenic | 55.0% | |
| ATGTCCGGATGCAGAAACGG+AGG | - | chr3.3:57137331-57137350 | None:intergenic | 55.0% | |
| GACACCACGAACTCCATCCT+TGG | - | chr3.3:57137085-57137104 | None:intergenic | 55.0% | |
| GGACGTCAAGAATGGAGAGG+GGG | + | chr3.3:57137146-57137165 | MS.gene55680:CDS | 55.0% | |
| GGGACGTCAAGAATGGAGAG+GGG | + | chr3.3:57137145-57137164 | MS.gene55680:CDS | 55.0% | |
| GGGGACGTCAAGAATGGAGA+GGG | + | chr3.3:57137144-57137163 | MS.gene55680:CDS | 55.0% | |
| GTAGAGGGGGACGTCAAGAA+TGG | + | chr3.3:57137138-57137157 | MS.gene55680:CDS | 55.0% | |
| GTCAAGAATGGAGAGGGGGA+TGG | + | chr3.3:57137150-57137169 | MS.gene55680:CDS | 55.0% | |
| ! | GGATGGACTCAAGGTTGCAC+AGG | + | chr3.3:57137167-57137186 | MS.gene55680:CDS | 55.0% |
| !!! | CGTTTTAGGGGTGGTAGAGG+GGG | + | chr3.3:57137125-57137144 | MS.gene55680:CDS | 55.0% |
| AGGCTCGGGAAGCAGAACCT+TGG | - | chr3.3:57137311-57137330 | None:intergenic | 60.0% | |
| CGAGCCTCCGTTTCTGCATC+CGG | + | chr3.3:57137324-57137343 | MS.gene55680:CDS | 60.0% | |
| CGGATGCAGAAACGGAGGCT+CGG | - | chr3.3:57137326-57137345 | None:intergenic | 60.0% | |
| GGATGCAGAAACGGAGGCTC+GGG | - | chr3.3:57137325-57137344 | None:intergenic | 60.0% | |
| GGGGGACGTCAAGAATGGAG+AGG | + | chr3.3:57137143-57137162 | MS.gene55680:CDS | 60.0% | |
| ! | GGAGTTCGTGGTGTCGAGGA+AGG | + | chr3.3:57137090-57137109 | MS.gene55680:CDS | 60.0% |
| ! | GGATGGAGTTCGTGGTGTCG+AGG | + | chr3.3:57137086-57137105 | MS.gene55680:CDS | 60.0% |
| ! | TGGAGAGGGGGATGGACTCA+AGG | + | chr3.3:57137158-57137177 | MS.gene55680:CDS | 60.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr3.3 | gene | 57137075 | 57137380 | 57137075 | ID=MS.gene55680 |
| chr3.3 | mRNA | 57137075 | 57137380 | 57137075 | ID=MS.gene55680.t1;Parent=MS.gene55680 |
| chr3.3 | exon | 57137075 | 57137380 | 57137075 | ID=MS.gene55680.t1.exon1;Parent=MS.gene55680.t1 |
| chr3.3 | CDS | 57137075 | 57137380 | 57137075 | ID=cds.MS.gene55680.t1;Parent=MS.gene55680.t1 |
| Gene Sequence |
| Protein sequence |
