Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene049631.t1 | KEH34139.1 | 43.5 | 124 | 41 | 3 | 1 | 100 | 1 | 119 | 2.20E-12 | 81.6 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene049631.t1 | A0A072UWC0 | 43.5 | 124 | 41 | 3 | 1 | 100 | 1 | 119 | 1.6e-12 | 81.6 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene049631 | MS.gene065391 | -0.80861 | 2.85E-50 | -1.69E-46 |
| MS.gene049631 | MS.gene24628 | 0.800352 | 1.50E-48 | -1.69E-46 |
| MS.gene049631 | MS.gene54043 | 0.805345 | 1.40E-49 | -1.69E-46 |
| MS.gene049631 | MS.gene55680 | 0.878376 | 2.95E-69 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene049631.t1 | MTR_3g462080 | 44.355 | 124 | 40 | 4 | 1 | 100 | 1 | 119 | 4.20e-22 | 83.6 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Find 27 sgRNAs with CRISPR-Local
Find 39 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| AGGAGATGTTTACTCGTTAT+TGG | 0.269194 | 3.2:+59237633 | MS.gene049631:CDS |
| TACTACCCTCCATACCCTTC+TGG | 0.348454 | 3.2:-59237658 | None:intergenic |
| CGAGCCTCCGTTTCTGCATC+CGG | 0.409336 | 3.2:+59237770 | MS.gene049631:CDS |
| GGATGCAGAAACGGAGGCTC+GGG | 0.468227 | 3.2:-59237768 | None:intergenic |
| AACATGTCCGGATGCAGAAA+CGG | 0.483189 | 3.2:-59237777 | None:intergenic |
| AGCAGCAGCAGCAACATGTC+CGG | 0.500108 | 3.2:-59237789 | None:intergenic |
| CGGATGCAGAAACGGAGGCT+CGG | 0.503641 | 3.2:-59237769 | None:intergenic |
| TACTCGTTATTGGTTCCAGA+AGG | 0.510115 | 3.2:+59237643 | MS.gene049631:CDS |
| GACACCACGAACTCCATCCT+TGG | 0.528708 | 3.2:-59237528 | None:intergenic |
| TTATTGGTTCCAGAAGGGTA+TGG | 0.546823 | 3.2:+59237649 | MS.gene049631:CDS |
| GGAGTTCGTGGTGTCGAGGA+AGG | 0.547698 | 3.2:+59237536 | MS.gene049631:CDS |
| GGGACGTCAAGAATGGAGAG+GGG | 0.556376 | 3.2:+59237591 | MS.gene049631:CDS |
| GATACCAAGGATGGAGTTCG+TGG | 0.560422 | 3.2:+59237524 | MS.gene049631:CDS |
| GGATGGACTCAAGGTTGCAC+AGG | 0.574542 | 3.2:+59237613 | MS.gene049631:CDS |
| GCTGTTGATTGTTTCAACCA+AGG | 0.576773 | 3.2:+59237737 | MS.gene049631:CDS |
| GGGGACGTCAAGAATGGAGA+GGG | 0.581994 | 3.2:+59237590 | MS.gene049631:CDS |
| GGACGTCAAGAATGGAGAGG+GGG | 0.582490 | 3.2:+59237592 | MS.gene049631:CDS |
| TGCAAAAGGAATGGATACCA+AGG | 0.584177 | 3.2:+59237511 | None:intergenic |
| AGGCTCGGGAAGCAGAACCT+TGG | 0.590344 | 3.2:-59237754 | None:intergenic |
| AAAGGAATGGATACCAAGGA+TGG | 0.599291 | 3.2:+59237515 | None:intergenic |
| TGGTTCCAGAAGGGTATGGA+GGG | 0.601975 | 3.2:+59237653 | MS.gene049631:CDS |
| GGATGGAGTTCGTGGTGTCG+AGG | 0.604594 | 3.2:+59237532 | MS.gene049631:CDS |
| TTGGTTCCAGAAGGGTATGG+AGG | 0.608678 | 3.2:+59237652 | MS.gene049631:CDS |
| AACATAGTCTACTCCTCACT+CGG | 0.615857 | 3.2:-59237819 | None:intergenic |
| ACTCGTTATTGGTTCCAGAA+GGG | 0.618430 | 3.2:+59237644 | MS.gene049631:CDS |
| GCTGCTACTGATACCGAGTG+AGG | 0.682392 | 3.2:+59237806 | MS.gene049631:CDS |
| ATGTCCGGATGCAGAAACGG+AGG | 0.716074 | 3.2:-59237774 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| AGGAGATGTTTACTCGTTAT+TGG | + | chr3.2:59237633-59237652 | MS.gene049631:CDS | 35.0% | |
| !! | AAGTCCAGCTTTTTGAAAGA+TGG | - | chr3.2:59237699-59237718 | None:intergenic | 35.0% |
| ! | AGTTCGTGATCTCGTTTTAG+GGG | + | chr3.2:59237559-59237578 | MS.gene049631:CDS | 40.0% |
| ! | GAGTTCGTGATCTCGTTTTA+GGG | + | chr3.2:59237558-59237577 | MS.gene049631:CDS | 40.0% |
| ! | GCTGTTGATTGTTTCAACCA+AGG | + | chr3.2:59237737-59237756 | MS.gene049631:CDS | 40.0% |
| !! | ACTCGTTATTGGTTCCAGAA+GGG | + | chr3.2:59237644-59237663 | MS.gene049631:CDS | 40.0% |
| !! | TACTCGTTATTGGTTCCAGA+AGG | + | chr3.2:59237643-59237662 | MS.gene049631:CDS | 40.0% |
| !! | TTATTGGTTCCAGAAGGGTA+TGG | + | chr3.2:59237649-59237668 | MS.gene049631:CDS | 40.0% |
| !! | TTTTGAAAGATGGCAGGTGA+TGG | - | chr3.2:59237689-59237708 | None:intergenic | 40.0% |
| AACATGTCCGGATGCAGAAA+CGG | - | chr3.2:59237780-59237799 | None:intergenic | 45.0% | |
| CCTGCCATCTTTCAAAAAGC+TGG | + | chr3.2:59237692-59237711 | MS.gene049631:CDS | 45.0% | |
| ! | GGAGTTCGTGATCTCGTTTT+AGG | + | chr3.2:59237557-59237576 | MS.gene049631:CDS | 45.0% |
| !! | CCAGCTTTTTGAAAGATGGC+AGG | - | chr3.2:59237695-59237714 | None:intergenic | 45.0% |
| GATACCAAGGATGGAGTTCG+TGG | + | chr3.2:59237524-59237543 | MS.gene049631:CDS | 50.0% | |
| TACTACCCTCCATACCCTTC+TGG | - | chr3.2:59237661-59237680 | None:intergenic | 50.0% | |
| ! | TGGTTCCAGAAGGGTATGGA+GGG | + | chr3.2:59237653-59237672 | MS.gene049631:CDS | 50.0% |
| ! | TTGGTTCCAGAAGGGTATGG+AGG | + | chr3.2:59237652-59237671 | MS.gene049631:CDS | 50.0% |
| !!! | CTCGTTTTAGGGGTGGTAGA+GGG | + | chr3.2:59237569-59237588 | MS.gene049631:CDS | 50.0% |
| !!! | TCGTGATCTCGTTTTAGGGG+TGG | + | chr3.2:59237562-59237581 | MS.gene049631:CDS | 50.0% |
| !!! | TCGTTTTAGGGGTGGTAGAG+GGG | + | chr3.2:59237570-59237589 | MS.gene049631:CDS | 50.0% |
| !!! | TCTCGTTTTAGGGGTGGTAG+AGG | + | chr3.2:59237568-59237587 | MS.gene049631:CDS | 50.0% |
| AGCAGCAGCAGCAACATGTC+CGG | - | chr3.2:59237792-59237811 | None:intergenic | 55.0% | |
| ATGTCCGGATGCAGAAACGG+AGG | - | chr3.2:59237777-59237796 | None:intergenic | 55.0% | |
| GACACCACGAACTCCATCCT+TGG | - | chr3.2:59237531-59237550 | None:intergenic | 55.0% | |
| GGACGTCAAGAATGGAGAGG+GGG | + | chr3.2:59237592-59237611 | MS.gene049631:CDS | 55.0% | |
| GGGACGTCAAGAATGGAGAG+GGG | + | chr3.2:59237591-59237610 | MS.gene049631:CDS | 55.0% | |
| GGGGACGTCAAGAATGGAGA+GGG | + | chr3.2:59237590-59237609 | MS.gene049631:CDS | 55.0% | |
| GTAGAGGGGGACGTCAAGAA+TGG | + | chr3.2:59237584-59237603 | MS.gene049631:CDS | 55.0% | |
| GTCAAGAATGGAGAGGGGGA+TGG | + | chr3.2:59237596-59237615 | MS.gene049631:CDS | 55.0% | |
| ! | GGATGGACTCAAGGTTGCAC+AGG | + | chr3.2:59237613-59237632 | MS.gene049631:CDS | 55.0% |
| !!! | CGTTTTAGGGGTGGTAGAGG+GGG | + | chr3.2:59237571-59237590 | MS.gene049631:CDS | 55.0% |
| AGGCTCGGGAAGCAGAACCT+TGG | - | chr3.2:59237757-59237776 | None:intergenic | 60.0% | |
| CGAGCCTCCGTTTCTGCATC+CGG | + | chr3.2:59237770-59237789 | MS.gene049631:CDS | 60.0% | |
| CGGATGCAGAAACGGAGGCT+CGG | - | chr3.2:59237772-59237791 | None:intergenic | 60.0% | |
| GGATGCAGAAACGGAGGCTC+GGG | - | chr3.2:59237771-59237790 | None:intergenic | 60.0% | |
| GGGGGACGTCAAGAATGGAG+AGG | + | chr3.2:59237589-59237608 | MS.gene049631:CDS | 60.0% | |
| ! | GGAGTTCGTGGTGTCGAGGA+AGG | + | chr3.2:59237536-59237555 | MS.gene049631:CDS | 60.0% |
| ! | GGATGGAGTTCGTGGTGTCG+AGG | + | chr3.2:59237532-59237551 | MS.gene049631:CDS | 60.0% |
| ! | TGGAGAGGGGGATGGACTCA+AGG | + | chr3.2:59237604-59237623 | MS.gene049631:CDS | 60.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr3.2 | gene | 59237521 | 59237826 | 59237521 | ID=MS.gene049631 |
| chr3.2 | mRNA | 59237521 | 59237826 | 59237521 | ID=MS.gene049631.t1;Parent=MS.gene049631 |
| chr3.2 | exon | 59237521 | 59237826 | 59237521 | ID=MS.gene049631.t1.exon1;Parent=MS.gene049631.t1 |
| chr3.2 | CDS | 59237521 | 59237826 | 59237521 | ID=cds.MS.gene049631.t1;Parent=MS.gene049631.t1 |
| Gene Sequence |
| Protein sequence |
