Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene55691.t1 | KEH34542.1 | 88.2 | 161 | 17 | 1 | 1 | 159 | 1 | 161 | 2.70E-73 | 284.6 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene55691.t1 | F4KEV7 | 60.7 | 150 | 57 | 1 | 6 | 155 | 28 | 175 | 1.6e-42 | 173.7 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene55691.t1 | A0A072UY82 | 88.2 | 161 | 17 | 1 | 1 | 159 | 1 | 161 | 2.0e-73 | 284.6 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene055942 | MS.gene55691 | 0.826279 | 2.96E-54 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene55691.t1 | MTR_3g063030 | 88.199 | 161 | 17 | 1 | 1 | 159 | 1 | 161 | 6.12e-103 | 292 |
| MS.gene55691.t1 | MTR_3g062950 | 78.698 | 169 | 22 | 3 | 1 | 159 | 1 | 165 | 2.01e-87 | 253 |
| MS.gene55691.t1 | MTR_3g062940 | 65.217 | 161 | 54 | 1 | 1 | 159 | 36 | 196 | 4.97e-62 | 189 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene55691.t1 | AT5G58240 | 59.868 | 152 | 59 | 1 | 6 | 157 | 8 | 157 | 1.55e-59 | 182 |
| MS.gene55691.t1 | AT5G58240 | 59.868 | 152 | 59 | 1 | 6 | 157 | 28 | 177 | 2.11e-59 | 182 |
| MS.gene55691.t1 | AT5G58240 | 66.667 | 111 | 37 | 0 | 47 | 157 | 15 | 125 | 8.36e-49 | 154 |
Find 25 sgRNAs with CRISPR-Local
Find 100 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| ATGATGAGAACATTGATTTA+TGG | 0.249450 | 3.3:+57007778 | MS.gene55691:CDS |
| GCTGTTGAATACTATGATTT+TGG | 0.282921 | 3.3:+57006370 | MS.gene55691:CDS |
| CAATGATGACATTTATGATT+TGG | 0.306333 | 3.3:+57008077 | MS.gene55691:CDS |
| CTTCTAGTGCCATTTCTTCA+AGG | 0.330045 | 3.3:-57008267 | None:intergenic |
| TGGATTTAGAGAGGAAAGAT+AGG | 0.366670 | 3.3:+57008242 | MS.gene55691:CDS |
| AAAAGGAGAAGGAGTTGAAA+AGG | 0.397103 | 3.3:+57008209 | MS.gene55691:CDS |
| AGATAGGAGCCTTGAAGAAA+TGG | 0.398545 | 3.3:+57008258 | MS.gene55691:CDS |
| GGGGTTTAGATTTAGAGATA+TGG | 0.433595 | 3.3:+57007040 | MS.gene55691:CDS |
| ATGTAAGTGATGATGCATTA+TGG | 0.437542 | 3.3:-57007839 | None:intergenic |
| TTGTATGAAACACTGAACTA+TGG | 0.499435 | 3.3:-57006408 | None:intergenic |
| AACCAGGATGGACCTCAAGC+TGG | 0.511868 | 3.3:+57007994 | MS.gene55691:intron |
| GCAGATAAATGAAAAGGAGA+AGG | 0.524751 | 3.3:+57008198 | MS.gene55691:intron |
| AACCTACGTCCTGCTGTCAC+TGG | 0.530201 | 3.3:+57006454 | MS.gene55691:CDS |
| AATGATGCAGATAAATGAAA+AGG | 0.533909 | 3.3:+57008192 | MS.gene55691:intron |
| TTCATATCCATATACTTCCT+CGG | 0.540594 | 3.3:+57008034 | MS.gene55691:CDS |
| TGGCGCATAGCACATAAACT+TGG | 0.555803 | 3.3:+57007798 | MS.gene55691:CDS |
| CAAGACATACCAGTGACAGC+AGG | 0.605749 | 3.3:-57006463 | None:intergenic |
| GGAACTGATTGTCCAGCTTG+AGG | 0.613109 | 3.3:-57008006 | None:intergenic |
| AGCTAGCTGTGGATTTAGAG+AGG | 0.627680 | 3.3:+57008233 | MS.gene55691:CDS |
| AGTATATGGATATGAACATG+AGG | 0.648556 | 3.3:-57008027 | None:intergenic |
| GTTGAAAAGGAAGCTAGCTG+TGG | 0.662335 | 3.3:+57008222 | MS.gene55691:CDS |
| AGCAGGACGTAGGTTGACAA+AGG | 0.667098 | 3.3:-57006446 | None:intergenic |
| AGATTTAGAGATATGGTACG+TGG | 0.685551 | 3.3:+57007047 | MS.gene55691:CDS |
| TATGAAACACTGAACTATGG+TGG | 0.696537 | 3.3:-57006405 | None:intergenic |
| TACCAGTGACAGCAGGACGT+AGG | 0.754574 | 3.3:-57006456 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | AAAAAAATCCTTAAATATTT+AGG | - | chr3.3:57007017-57007036 | None:intergenic | 10.0% |
| !! | ATTAATCTATCTTTATAATA+TGG | + | chr3.3:57007078-57007097 | MS.gene55691:intron | 10.0% |
| !! | TGTTAATTAAAAAAAAAAGT+TGG | + | chr3.3:57006597-57006616 | MS.gene55691:intron | 10.0% |
| !!! | AATATTTAAGGATTTTTTTT+AGG | + | chr3.3:57007018-57007037 | MS.gene55691:intron | 10.0% |
| !!! | ATATTTAAGGAATTTTTTTA+AGG | + | chr3.3:57006777-57006796 | MS.gene55691:intron | 10.0% |
| !!! | ATATTTAAGGATTTTTTTTA+GGG | + | chr3.3:57007019-57007038 | MS.gene55691:intron | 10.0% |
| !!! | TATTTAAGGAATTTTTTTAA+GGG | + | chr3.3:57006778-57006797 | MS.gene55691:intron | 10.0% |
| !! | AAATAGTGTCTAAATATTTA+AGG | + | chr3.3:57006764-57006783 | MS.gene55691:intron | 15.0% |
| !! | CTAAAAAACAAGTAAAAATT+TGG | + | chr3.3:57008150-57008169 | MS.gene55691:intron | 15.0% |
| !! | TTCTCAAAATCTTAATATTA+AGG | + | chr3.3:57007607-57007626 | MS.gene55691:intron | 15.0% |
| !!! | ATTTAAGGAATTTTTTTAAG+GGG | + | chr3.3:57006779-57006798 | MS.gene55691:intron | 15.0% |
| !!! | CATTTGTGTTTTTAATAATT+TGG | + | chr3.3:57008110-57008129 | MS.gene55691:intron | 15.0% |
| !!! | TATTGTTATTTGAGTATATA+AGG | + | chr3.3:57007891-57007910 | MS.gene55691:intron | 15.0% |
| !!! | TATTTAAGGATTTTTTTTAG+GGG | + | chr3.3:57007020-57007039 | MS.gene55691:intron | 15.0% |
| !! | AAAGAAAGAAATTATTGTGA+GGG | + | chr3.3:57007347-57007366 | MS.gene55691:intron | 20.0% |
| !! | ATTAATCTATCTTTACAATC+TGG | + | chr3.3:57006850-57006869 | MS.gene55691:intron | 20.0% |
| !! | CTAAAATCATTGATAAAAAG+AGG | + | chr3.3:57007400-57007419 | MS.gene55691:intron | 20.0% |
| !! | TAATTAACATCAGACTAAAA+AGG | - | chr3.3:57006586-57006605 | None:intergenic | 20.0% |
| !! | TTTCTTTCTTTATAAACTAG+AGG | - | chr3.3:57007334-57007353 | None:intergenic | 20.0% |
| !!! | ATTTAAGGATTTTTTTTAGG+GGG | + | chr3.3:57007021-57007040 | MS.gene55691:intron | 20.0% |
| !!! | ATTTTGTTAGAAATTTGTCT+GGG | + | chr3.3:57007513-57007532 | MS.gene55691:intron | 20.0% |
| !!! | TCAATTCTGTTTTTTTATCT+TGG | + | chr3.3:57006684-57006703 | MS.gene55691:intron | 20.0% |
| !!! | TTGTAATCTTTTACTATTTG+AGG | - | chr3.3:57007237-57007256 | None:intergenic | 20.0% |
| ! | AATGATGCAGATAAATGAAA+AGG | + | chr3.3:57008192-57008211 | MS.gene55691:intron | 25.0% |
| ! | AATGTAAACATGAATCTACT+TGG | + | chr3.3:57007659-57007678 | MS.gene55691:intron | 25.0% |
| ! | ACATAGTGCCTAAATATTTA+AGG | + | chr3.3:57007006-57007025 | MS.gene55691:intron | 25.0% |
| ! | ATTCTTCAAATTGTCTTACT+TGG | - | chr3.3:57007872-57007891 | None:intergenic | 25.0% |
| ! | CAATGATGACATTTATGATT+TGG | + | chr3.3:57008077-57008096 | MS.gene55691:CDS | 25.0% |
| ! | CTTTATAATATGGTAAGTGT+CGG | + | chr3.3:57007088-57007107 | MS.gene55691:intron | 25.0% |
| ! | GAAAGAAAGAAATTATTGTG+AGG | + | chr3.3:57007346-57007365 | MS.gene55691:intron | 25.0% |
| ! | TACAACAACATTTGATATGT+CGG | + | chr3.3:57007252-57007271 | MS.gene55691:intron | 25.0% |
| ! | TATTTAAGCTTGAAGATCAT+AGG | - | chr3.3:57006518-57006537 | None:intergenic | 25.0% |
| !! | CATTTTGTTAGAAATTTGTC+TGG | + | chr3.3:57007512-57007531 | MS.gene55691:intron | 25.0% |
| !! | TGAATTTGATTGACTTACTT+AGG | + | chr3.3:57006948-57006967 | MS.gene55691:intron | 25.0% |
| !!! | ATGATGAGAACATTGATTTA+TGG | + | chr3.3:57007778-57007797 | MS.gene55691:CDS | 25.0% |
| AATAATAGATCAAAGAGGTG+AGG | - | chr3.3:57006492-57006511 | None:intergenic | 30.0% | |
| ACAAAACAAGTGCTTAAAAG+TGG | - | chr3.3:57007303-57007322 | None:intergenic | 30.0% | |
| ACAACAACATTTGATATGTC+CGG | + | chr3.3:57006915-57006934 | MS.gene55691:intron | 30.0% | |
| ACAACAACATTTGATATGTC+GGG | + | chr3.3:57006915-57006934 | MS.gene55691:intron | 30.0% | |
| AGTATATGGATATGAACATG+AGG | - | chr3.3:57008030-57008049 | None:intergenic | 30.0% | |
| ATAATAGATCAAAGAGGTGA+GGG | - | chr3.3:57006491-57006510 | None:intergenic | 30.0% | |
| ATTGTATAGCCATATAGCAT+TGG | - | chr3.3:57007945-57007964 | None:intergenic | 30.0% | |
| GCTGTATTTGTAATCACATA+AGG | + | chr3.3:57007575-57007594 | MS.gene55691:intron | 30.0% | |
| GCTTCATCATATAATCACAT+TGG | + | chr3.3:57007472-57007491 | MS.gene55691:intron | 30.0% | |
| TCAAATTCAGCTTCAAAATC+CGG | - | chr3.3:57006937-57006956 | None:intergenic | 30.0% | |
| TGGTACGTAGTAATACATAT+CGG | + | chr3.3:57006818-57006837 | MS.gene55691:intron | 30.0% | |
| TTCATATCCATATACTTCCT+CGG | + | chr3.3:57008034-57008053 | MS.gene55691:CDS | 30.0% | |
| TTGTATGAAACACTGAACTA+TGG | - | chr3.3:57006411-57006430 | None:intergenic | 30.0% | |
| TTTGTAATCACATAAGGCTA+TGG | + | chr3.3:57007581-57007600 | MS.gene55691:intron | 30.0% | |
| ! | AATTGACACACTCTTTTGTT+AGG | + | chr3.3:57007373-57007392 | MS.gene55691:intron | 30.0% |
| ! | ATGTAAGTGATGATGCATTA+TGG | - | chr3.3:57007842-57007861 | None:intergenic | 30.0% |
| ! | GCTGTTGAATACTATGATTT+TGG | + | chr3.3:57006370-57006389 | MS.gene55691:CDS | 30.0% |
| !! | ACATTTATGATTTGGTAGGA+TGG | + | chr3.3:57008085-57008104 | MS.gene55691:intron | 30.0% |
| !! | GATGACATTTATGATTTGGT+AGG | + | chr3.3:57008081-57008100 | MS.gene55691:intron | 30.0% |
| !! | TTACTATTTGAGTTGTACCT+AGG | - | chr3.3:57007130-57007149 | None:intergenic | 30.0% |
| !!! | AGATGCTATCTTTTGAGTTT+GGG | - | chr3.3:57007439-57007458 | None:intergenic | 30.0% |
| !!! | TTTTAAGCACTTGTTTTGTG+AGG | + | chr3.3:57007304-57007323 | MS.gene55691:intron | 30.0% |
| AAAAGGAGAAGGAGTTGAAA+AGG | + | chr3.3:57008209-57008228 | MS.gene55691:CDS | 35.0% | |
| ACAACAAGTGCTTAAAAGTG+GGG | - | chr3.3:57007204-57007223 | None:intergenic | 35.0% | |
| ACACAACAAGTGCTTAAAAG+TGG | - | chr3.3:57007206-57007225 | None:intergenic | 35.0% | |
| AGATTTAGAGATATGGTACG+TGG | + | chr3.3:57006805-57006824 | MS.gene55691:intron | 35.0% | |
| AGCATATTCGAATCAACAAC+TGG | - | chr3.3:57007178-57007197 | None:intergenic | 35.0% | |
| ATGACCTGCGAAAGATAAAA+AGG | - | chr3.3:57006561-57006580 | None:intergenic | 35.0% | |
| CAAATCAATCTTTCTGAACC+AGG | + | chr3.3:57007978-57007997 | MS.gene55691:intron | 35.0% | |
| CACAACAAGTGCTTAAAAGT+GGG | - | chr3.3:57007205-57007224 | None:intergenic | 35.0% | |
| CATATACTTCCTCGGAAAAA+CGG | + | chr3.3:57008042-57008061 | MS.gene55691:CDS | 35.0% | |
| CTGGTTCAGAAAGATTGATT+TGG | - | chr3.3:57007980-57007999 | None:intergenic | 35.0% | |
| CTTTACAATCTGGTAAGTGT+GGG | + | chr3.3:57006860-57006879 | MS.gene55691:intron | 35.0% | |
| GCAGATAAATGAAAAGGAGA+AGG | + | chr3.3:57008198-57008217 | MS.gene55691:intron | 35.0% | |
| GGAGCAATAATAGATCAAAG+AGG | - | chr3.3:57006497-57006516 | None:intergenic | 35.0% | |
| GGGGTTTAGATTTAGAGATA+TGG | + | chr3.3:57006798-57006817 | MS.gene55691:intron | 35.0% | |
| GGTACGTAGTAATACATATC+GGG | + | chr3.3:57006819-57006838 | MS.gene55691:intron | 35.0% | |
| TATGAAACACTGAACTATGG+TGG | - | chr3.3:57006408-57006427 | None:intergenic | 35.0% | |
| TCTTTACAATCTGGTAAGTG+TGG | + | chr3.3:57006859-57006878 | MS.gene55691:intron | 35.0% | |
| TGGATTTAGAGAGGAAAGAT+AGG | + | chr3.3:57008242-57008261 | MS.gene55691:CDS | 35.0% | |
| ! | CTTTCCTTTTTATCTTTCGC+AGG | + | chr3.3:57006554-57006573 | MS.gene55691:intron | 35.0% |
| !!! | GAGATGCTATCTTTTGAGTT+TGG | - | chr3.3:57007440-57007459 | None:intergenic | 35.0% |
| !!! | TTTTAAGCACTTGTTGTGTG+AGG | + | chr3.3:57007207-57007226 | MS.gene55691:intron | 35.0% |
| AGATAGGAGCCTTGAAGAAA+TGG | + | chr3.3:57008258-57008277 | MS.gene55691:CDS | 40.0% | |
| GCTTAAAAGTGGAGAATAGC+AGG | - | chr3.3:57007292-57007311 | None:intergenic | 40.0% | |
| TCAATCTTTCTGAACCAGGA+TGG | + | chr3.3:57007982-57008001 | MS.gene55691:intron | 40.0% | |
| TGCCTAAGACCAATGCTATA+TGG | + | chr3.3:57007933-57007952 | MS.gene55691:intron | 40.0% | |
| TTAGAGATATGGTACGTGGT+AGG | + | chr3.3:57007051-57007070 | MS.gene55691:intron | 40.0% | |
| !! | AGCCATATAGCATTGGTCTT+AGG | - | chr3.3:57007938-57007957 | None:intergenic | 40.0% |
| !! | CGTTTTTCCGAGGAAGTATA+TGG | - | chr3.3:57008044-57008063 | None:intergenic | 40.0% |
| !! | CTTCTAGTGCCATTTCTTCA+AGG | - | chr3.3:57008270-57008289 | None:intergenic | 40.0% |
| AGCTAGCTGTGGATTTAGAG+AGG | + | chr3.3:57008233-57008252 | MS.gene55691:CDS | 45.0% | |
| GTTGAAAAGGAAGCTAGCTG+TGG | + | chr3.3:57008222-57008241 | MS.gene55691:CDS | 45.0% | |
| TGGCGCATAGCACATAAACT+TGG | + | chr3.3:57007798-57007817 | MS.gene55691:CDS | 45.0% | |
| !! | TCGAAATCACCGTTTTTCCG+AGG | - | chr3.3:57008054-57008073 | None:intergenic | 45.0% |
| CAAGACATACCAGTGACAGC+AGG | - | chr3.3:57006466-57006485 | None:intergenic | 50.0% | |
| GGAACTGATTGTCCAGCTTG+AGG | - | chr3.3:57008009-57008028 | None:intergenic | 50.0% | |
| GTCTCGTAGGTACAACTCCT+AGG | + | chr3.3:57007110-57007129 | MS.gene55691:intron | 50.0% | |
| ! | AGCAGGACGTAGGTTGACAA+AGG | - | chr3.3:57006449-57006468 | None:intergenic | 50.0% |
| !! | ATGGTAAGTGTCGGTCTCGT+AGG | + | chr3.3:57007097-57007116 | MS.gene55691:intron | 50.0% |
| AACCAGGATGGACCTCAAGC+TGG | + | chr3.3:57007994-57008013 | MS.gene55691:intron | 55.0% | |
| AACCTACGTCCTGCTGTCAC+TGG | + | chr3.3:57006454-57006473 | MS.gene55691:CDS | 55.0% | |
| ACACGCTTCACTTCGCGCTT+CGG | - | chr3.3:57007744-57007763 | None:intergenic | 55.0% | |
| TACCAGTGACAGCAGGACGT+AGG | - | chr3.3:57006459-57006478 | None:intergenic | 55.0% | |
| GTCCAGCTTGAGGTCCATCC+TGG | - | chr3.3:57007999-57008018 | None:intergenic | 60.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr3.3 | gene | 57006367 | 57008318 | 57006367 | ID=MS.gene55691 |
| chr3.3 | mRNA | 57006367 | 57008318 | 57006367 | ID=MS.gene55691.t1;Parent=MS.gene55691 |
| chr3.3 | exon | 57006367 | 57006475 | 57006367 | ID=MS.gene55691.t1.exon1;Parent=MS.gene55691.t1 |
| chr3.3 | CDS | 57006367 | 57006475 | 57006367 | ID=cds.MS.gene55691.t1;Parent=MS.gene55691.t1 |
| chr3.3 | exon | 57007040 | 57007068 | 57007040 | ID=MS.gene55691.t1.exon2;Parent=MS.gene55691.t1 |
| chr3.3 | CDS | 57007040 | 57007068 | 57007040 | ID=cds.MS.gene55691.t1;Parent=MS.gene55691.t1 |
| chr3.3 | exon | 57007747 | 57007872 | 57007747 | ID=MS.gene55691.t1.exon3;Parent=MS.gene55691.t1 |
| chr3.3 | CDS | 57007747 | 57007872 | 57007747 | ID=cds.MS.gene55691.t1;Parent=MS.gene55691.t1 |
| chr3.3 | exon | 57008000 | 57008098 | 57008000 | ID=MS.gene55691.t1.exon4;Parent=MS.gene55691.t1 |
| chr3.3 | CDS | 57008000 | 57008098 | 57008000 | ID=cds.MS.gene55691.t1;Parent=MS.gene55691.t1 |
| chr3.3 | exon | 57008202 | 57008318 | 57008202 | ID=MS.gene55691.t1.exon5;Parent=MS.gene55691.t1 |
| chr3.3 | CDS | 57008202 | 57008318 | 57008202 | ID=cds.MS.gene55691.t1;Parent=MS.gene55691.t1 |
| Gene Sequence |
| Protein sequence |
