AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene56015

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene56015.t1 MTR_2g034600 96.646 477 16 0 1 477 20 496 0.0 948
MS.gene56015.t1 MTR_2g034600 96.646 477 16 0 1 477 85 561 0.0 948
MS.gene56015.t1 MTR_2g034600 95.807 477 16 1 1 477 85 557 0.0 933
MS.gene56015.t1 MTR_5g041630 33.636 220 140 5 262 476 87 305 2.38e-30 120
MS.gene56015.t1 MTR_6g003960 30.078 256 174 3 212 463 333 587 5.15e-30 123
MS.gene56015.t1 MTR_5g068860 29.455 275 184 6 198 465 213 484 2.27e-29 121
MS.gene56015.t1 MTR_5g068860 23.050 282 168 6 192 469 139 375 4.33e-13 71.6
MS.gene56015.t1 MTR_1g115415 29.474 285 189 6 192 468 175 455 7.30e-29 119
MS.gene56015.t1 MTR_1g115415 23.485 264 176 5 170 411 67 326 1.44e-13 73.2
MS.gene56015.t1 MTR_4g070060 24.913 289 208 7 192 473 309 595 1.51e-26 113
MS.gene56015.t1 MTR_4g070060 25.292 257 177 6 225 471 271 522 4.82e-20 93.6
MS.gene56015.t1 MTR_4g070060 29.949 197 135 3 189 383 378 573 1.04e-14 77.0
MS.gene56015.t1 MTR_4g084630 26.103 272 195 4 198 463 47 318 1.99e-26 109
MS.gene56015.t1 MTR_4g084630 24.424 217 153 3 268 476 10 223 1.28e-13 72.4
MS.gene56015.t1 MTR_4g084630 28.947 152 106 2 188 337 109 260 1.80e-12 68.9
MS.gene56015.t1 MTR_2g049780 30.992 242 152 6 242 469 70 310 3.32e-26 108
MS.gene56015.t1 MTR_5g094610 31.579 228 141 6 196 414 230 451 2.56e-25 108
MS.gene56015.t1 MTR_5g094610 27.468 233 139 7 251 459 177 403 2.11e-17 85.1
MS.gene56015.t1 MTR_5g094610 30.052 193 130 4 188 376 258 449 5.14e-17 84.0
MS.gene56015.t1 MTR_2g067230 29.333 225 147 5 248 467 86 303 8.54e-22 96.3
MS.gene56015.t1 MTR_3g092710 25.000 324 191 12 196 475 187 502 2.72e-20 94.0
MS.gene56015.t1 MTR_8g105560 25.948 343 196 9 189 475 155 495 3.88e-18 87.4
MS.gene56015.t1 MTR_8g105560 22.874 341 216 10 170 468 45 380 1.70e-12 70.1
MS.gene56015.t1 MTR_7g081270 29.756 205 132 5 268 463 78 279 4.57e-16 79.0
MS.gene56015.t1 MTR_4g119580 25.000 276 181 8 196 460 81 341 1.06e-12 69.7
MS.gene56015.t1 MTR_2g437100 25.498 251 174 7 196 439 99 343 5.22e-11 64.7
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene56015.t1 AT4G14605 63.901 446 150 4 29 464 24 468 0.0 560
MS.gene56015.t1 AT4G14605 63.901 446 150 4 29 464 24 468 0.0 560
MS.gene56015.t1 AT2G21710 28.912 294 194 7 192 475 316 604 3.40e-33 133
MS.gene56015.t1 AT2G21710 30.366 191 130 3 189 377 385 574 1.78e-13 73.2
MS.gene56015.t1 AT2G21710 28.114 281 182 7 58 337 306 567 4.31e-13 72.0
MS.gene56015.t1 AT2G44020 27.726 321 186 6 191 468 143 460 1.10e-29 122
MS.gene56015.t1 AT4G38160 27.372 274 186 5 202 465 52 322 2.37e-27 112
MS.gene56015.t1 AT4G38160 30.719 153 103 3 188 337 110 262 3.46e-14 73.9
MS.gene56015.t1 AT4G38160 27.372 274 186 5 202 465 52 322 2.37e-27 112
MS.gene56015.t1 AT4G38160 30.719 153 103 3 188 337 110 262 3.46e-14 73.9
MS.gene56015.t1 AT1G78930 28.125 256 179 3 212 463 320 574 3.26e-27 115
MS.gene56015.t1 AT4G38160 27.372 274 186 5 202 465 52 322 5.77e-27 112
MS.gene56015.t1 AT4G38160 30.719 153 103 3 188 337 110 262 4.71e-14 73.9
MS.gene56015.t1 AT4G38160 27.372 274 186 5 202 465 52 322 6.17e-27 112
MS.gene56015.t1 AT4G38160 30.719 153 103 3 188 337 110 262 5.33e-14 73.9
MS.gene56015.t1 AT2G34620 30.833 240 151 6 242 467 56 294 3.19e-25 105
MS.gene56015.t1 AT2G34620 28.972 214 143 7 170 379 66 274 2.28e-11 65.1
MS.gene56015.t1 AT2G21710 28.049 246 162 7 192 427 316 556 6.09e-24 105
MS.gene56015.t1 AT2G21710 25.229 218 156 2 228 438 245 462 3.79e-15 78.2
MS.gene56015.t1 AT2G21710 28.685 251 162 6 58 307 306 540 6.74e-13 71.2
MS.gene56015.t1 AT2G21710 31.847 157 104 3 189 343 385 540 4.21e-12 68.9
MS.gene56015.t1 AT2G03050 32.039 206 129 6 270 467 70 272 6.28e-24 101
MS.gene56015.t1 AT2G03050 25.346 217 140 8 168 379 53 252 5.08e-11 63.9
MS.gene56015.t1 AT2G03050 32.039 206 129 6 270 467 71 273 6.78e-24 101
MS.gene56015.t1 AT2G03050 25.346 217 140 8 168 379 54 253 5.52e-11 63.9
MS.gene56015.t1 AT2G21710 28.163 245 161 7 192 426 316 555 1.36e-23 104
MS.gene56015.t1 AT2G21710 25.229 218 156 2 228 438 245 462 3.05e-15 78.6
MS.gene56015.t1 AT2G21710 28.685 251 162 6 58 307 306 540 7.90e-13 71.2
MS.gene56015.t1 AT2G21710 31.847 157 104 3 189 343 385 540 3.96e-12 68.9
MS.gene56015.t1 AT4G02990 26.224 286 193 7 191 465 214 492 6.75e-23 102
MS.gene56015.t1 AT4G02990 25.909 220 145 4 170 375 115 330 9.95e-15 77.0
MS.gene56015.t1 AT4G02990 26.224 286 193 7 191 465 214 492 6.75e-23 102
MS.gene56015.t1 AT4G02990 25.909 220 145 4 170 375 115 330 9.95e-15 77.0
MS.gene56015.t1 AT3G18870 27.962 211 128 5 267 463 68 268 3.31e-20 90.9
MS.gene56015.t1 AT5G55580 25.941 239 162 7 196 422 223 458 3.92e-19 90.5
MS.gene56015.t1 AT5G55580 28.497 193 135 3 188 377 251 443 4.40e-18 87.0
MS.gene56015.t1 AT5G55580 23.864 264 188 8 185 440 180 438 3.33e-16 81.3
MS.gene56015.t1 AT5G55580 26.267 217 131 7 266 459 186 396 6.99e-13 71.2
MS.gene56015.t1 AT5G55580 25.000 188 131 5 268 445 223 410 1.63e-12 70.1
MS.gene56015.t1 AT5G55580 25.941 239 162 7 196 422 117 352 4.81e-19 89.4
MS.gene56015.t1 AT5G55580 28.497 193 135 3 188 377 145 337 3.70e-18 86.7
MS.gene56015.t1 AT5G55580 23.529 255 185 7 194 440 80 332 3.63e-16 80.5
MS.gene56015.t1 AT5G55580 25.778 225 138 7 258 459 72 290 5.06e-13 70.9
MS.gene56015.t1 AT5G55580 25.000 188 131 5 268 445 117 304 1.44e-12 69.7
MS.gene56015.t1 AT2G36000 29.082 196 126 5 279 467 122 311 1.03e-17 84.7
MS.gene56015.t1 AT2G36000 26.840 231 153 7 246 467 88 311 1.36e-17 84.0
MS.gene56015.t1 AT5G55580 27.097 155 110 3 196 347 223 377 2.17e-14 75.1
MS.gene56015.t1 AT5G55580 24.747 198 128 6 266 448 186 377 3.46e-12 68.6
MS.gene56015.t1 AT5G54180 24.555 281 171 9 189 465 247 490 1.41e-13 73.2
MS.gene56015.t1 AT1G79220 30.769 143 92 3 192 332 149 286 1.25e-11 66.6
MS.gene56015.t1 AT1G21150 24.815 270 181 7 196 456 56 312 1.81e-11 65.9
MS.gene56015.t1 AT1G21150 24.815 270 181 7 196 456 92 348 2.99e-11 65.5
MS.gene56015.t1 AT1G21150 24.815 270 181 7 196 456 156 412 6.06e-11 64.7
MS.gene56015.t1 AT1G62110 25.822 213 146 8 232 439 195 400 6.24e-11 64.7

Find 91 sgRNAs with CRISPR-Local

Find 159 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CACAAAACTTGGTACCTTTC+AGG 0.146574 2.3:-56235741 MS.gene56015:intron
TTCAATTGTCCACAAAATTT+TGG 0.188550 2.3:-56234912 MS.gene56015:CDS
AATTTAACCAGCTCAGATTT+TGG 0.213106 2.3:+56234723 None:intergenic
ATGAAATTCTTTGAATCTTT+AGG 0.253896 2.3:-56235170 MS.gene56015:CDS
GGATACACCGTGGAAGAAAT+TGG 0.270831 2.3:-56234837 MS.gene56015:CDS
TTATCCTTAACAGGTGGATT+TGG 0.286270 2.3:+56235524 None:intergenic
AAAGAAAATGCAAGTTGATT+AGG 0.286687 2.3:-56234559 None:intergenic
TTCTCATTATCCTCACTAAA+AGG 0.287136 2.3:-56235238 MS.gene56015:CDS
TTGTGTTACGGGCTTTATAT+TGG 0.339450 2.3:+56234875 None:intergenic
GAGAGAAGGTGATCAATAAA+AGG 0.354084 2.3:+56235780 None:intergenic
GGGATTATGATTATGAGATA+TGG 0.366062 2.3:-56234816 MS.gene56015:CDS
CTATCCAAATCCACCTGTTA+AGG 0.367312 2.3:-56235528 MS.gene56015:CDS
GCGTCCGATTTATCCTTAAC+AGG 0.379368 2.3:+56235515 None:intergenic
CTGAGAATTTGATGCCGAAA+TGG 0.384614 2.3:-56234770 MS.gene56015:CDS
TCCACCTGTTAAGGATAAAT+CGG 0.398567 2.3:-56235519 MS.gene56015:CDS
TAAAGATTCAAAGAATTTCA+TGG 0.398908 2.3:+56235172 None:intergenic
TTTACCCTCCAAACTATAGT+AGG 0.403290 2.3:+56235313 None:intergenic
ACACTGTAACTAACAATAGT+TGG 0.403606 2.3:+56235002 None:intergenic
GGTGAGATTACTGTTGAATC+AGG 0.408489 2.3:-56234631 MS.gene56015:CDS
AGCTTACAACTCTTGAGATC+AGG 0.409547 2.3:-56235622 MS.gene56015:CDS
CTAGGACTAGGAGGTGGAAT+TGG 0.414937 2.3:+56235491 None:intergenic
AAACTATAGTAGGCAAAAGC+TGG 0.426748 2.3:+56235323 None:intergenic
GTCGCATAATAGTCCTAATC+TGG 0.437513 2.3:+56235351 None:intergenic
GCAATGTACTTCCGTTCTCT+AGG 0.440973 2.3:-56234957 MS.gene56015:CDS
AACCCTATTAGCAGCAGAAA+AGG 0.445757 2.3:-56235889 MS.gene56015:CDS
CAATGTACTTCCGTTCTCTA+GGG 0.451044 2.3:-56234956 MS.gene56015:CDS
TGCTATAAGTTAGCAGGGCT+GGG 0.457629 2.3:+56235108 None:intergenic
GTGCTATAAGTTAGCAGGGC+TGG 0.458589 2.3:+56235107 None:intergenic
GTGGACAATTGAATAGAAGA+AGG 0.464746 2.3:+56234922 None:intergenic
TCCTCATATTGCTTATCTGA+TGG 0.465202 2.3:-56235393 MS.gene56015:CDS
TGAGAATTTGATGCCGAAAT+GGG 0.466974 2.3:-56234769 MS.gene56015:CDS
CTCAACTTCCTTCCTCTACA+AGG 0.472025 2.3:-56236084 MS.gene56015:CDS
GAATGAATATTGACCAGATT+AGG 0.481227 2.3:-56235364 MS.gene56015:CDS
GGACGAAGATTTCCCTCAGC+AGG 0.482343 2.3:+56235413 None:intergenic
TCTGCGTGCTATAAGTTAGC+AGG 0.485788 2.3:+56235102 None:intergenic
GATACACCGTGGAAGAAATT+GGG 0.490444 2.3:-56234836 MS.gene56015:CDS
AGGTGTTGACAAGGAACAAT+GGG 0.491795 2.3:-56235150 MS.gene56015:CDS
TCTGCTGCTAATAGGGTTGG+AGG 0.494961 2.3:+56235894 None:intergenic
ACTAAAAGGCCTCAATTATG+TGG 0.498259 2.3:-56235224 MS.gene56015:CDS
CACTGTAACTAACAATAGTT+GGG 0.500646 2.3:+56235003 None:intergenic
TAGAGTGAAGCTTCGAGAGA+AGG 0.507356 2.3:+56235766 None:intergenic
CATAATCCCAATTTCTTCCA+CGG 0.513081 2.3:+56234830 None:intergenic
AAGTTAGCAGGGCTGGGAAA+CGG 0.513243 2.3:+56235114 None:intergenic
CGTTCTCTAGGGGTTGATGT+TGG 0.515834 2.3:-56234945 MS.gene56015:CDS
AAACAAGGTTAATACAGCCT+TGG 0.517039 2.3:+56235860 None:intergenic
CTTCTGAGACACCAAATTCG+CGG 0.517645 2.3:+56235054 None:intergenic
TTCACTCTAAACACAAAACT+TGG 0.527791 2.3:-56235752 MS.gene56015:CDS
ATTGTGGGGTGGTTGAATTG+AGG 0.533017 2.3:+56236737 None:intergenic
ATGGCACGGAATGGAAATTG+TGG 0.536305 2.3:+56236721 None:intergenic
AACTATAGTAGGCAAAAGCT+GGG 0.542556 2.3:+56235324 None:intergenic
GGATTACCCAAAATCTGAGC+TGG 0.542893 2.3:-56234730 MS.gene56015:CDS
TTTGCCTACTATAGTTTGGA+GGG 0.546380 2.3:-56235317 MS.gene56015:CDS
AAGAAGACCCTTGTAGAGGA+AGG 0.547271 2.3:+56236076 None:intergenic
TCCATCAGATAAGCAATATG+AGG 0.550329 2.3:+56235392 None:intergenic
TTAGAGTCTACCGCTGGACT+AGG 0.566955 2.3:+56235473 None:intergenic
AGCAGAAAAGGAAGAAGCCA+AGG 0.567349 2.3:-56235877 MS.gene56015:CDS
GCTTACAACTCTTGAGATCA+GGG 0.568117 2.3:-56235621 MS.gene56015:CDS
TCCGATTTATCCTTAACAGG+TGG 0.568259 2.3:+56235518 None:intergenic
TGGCACGGAATGGAAATTGT+GGG 0.570971 2.3:+56236722 None:intergenic
GATTCAAAGAATTTCATGGT+GGG 0.572446 2.3:+56235176 None:intergenic
AGAAATTCTAGTGGATGTAG+TGG 0.577348 2.3:-56235555 MS.gene56015:CDS
AATGTACTTCCGTTCTCTAG+GGG 0.585781 2.3:-56234955 MS.gene56015:CDS
ACTTTCTTAGAGTCTACCGC+TGG 0.586538 2.3:+56235467 None:intergenic
GGAGGGTAAAATTAAGCCAG+TGG 0.594688 2.3:-56235300 MS.gene56015:CDS
AGAAAAGAAGACCCTTGTAG+AGG 0.600416 2.3:+56236072 None:intergenic
ATTGCTTATCTGATGGAACT+CGG 0.604771 2.3:-56235386 MS.gene56015:CDS
TGAATCTTTAGGTGTTGACA+AGG 0.607852 2.3:-56235159 MS.gene56015:CDS
AGACTGATTCCACATAATTG+AGG 0.610049 2.3:+56235215 None:intergenic
TAGGTGTTGACAAGGAACAA+TGG 0.611520 2.3:-56235151 MS.gene56015:CDS
TATTGTTAGTTACAGTGTAG+AGG 0.611669 2.3:-56234997 MS.gene56015:CDS
TTGTAGCAACCAAACCTGAA+AGG 0.613581 2.3:+56235727 None:intergenic
CAACATCAACCCCTAGAGAA+CGG 0.618334 2.3:+56234946 None:intergenic
CTGCGTGCTATAAGTTAGCA+GGG 0.619373 2.3:+56235103 None:intergenic
GAATCACTTTACGAAGAGCA+TGG 0.623459 2.3:-56235578 MS.gene56015:CDS
TATACCACTCACTCGAGACA+CGG 0.626260 2.3:+56235439 None:intergenic
ACCTCCTAGTCCTAGTCCAG+CGG 0.630206 2.3:-56235483 MS.gene56015:CDS
GACGAAGATTTCCCTCAGCA+GGG 0.635800 2.3:+56235414 None:intergenic
TCTACCGCTGGACTAGGACT+AGG 0.636230 2.3:+56235479 None:intergenic
GGTGTCTCAGAAGAGAACAT+AGG 0.641929 2.3:-56235044 MS.gene56015:CDS
AAAGCCGTGTCTCGAGTGAG+TGG 0.646198 2.3:-56235443 MS.gene56015:CDS
AGAGCATGGAGAAATTCTAG+TGG 0.648996 2.3:-56235564 MS.gene56015:CDS
GCTGGACTAGGACTAGGAGG+TGG 0.650924 2.3:+56235485 None:intergenic
GAGTGGTATAGACCCTGCTG+AGG 0.656302 2.3:-56235426 MS.gene56015:CDS
GAACGGAAGTACATTGCTGT+CGG 0.657257 2.3:+56234963 None:intergenic
TCAACTTCCTTCCTCTACAA+GGG 0.663747 2.3:-56236083 MS.gene56015:CDS
AGTGGTATAGACCCTGCTGA+GGG 0.666006 2.3:-56235425 MS.gene56015:CDS
ACCGCTGGACTAGGACTAGG+AGG 0.679156 2.3:+56235482 None:intergenic
AGATTCAAAGAATTTCATGG+TGG 0.685200 2.3:+56235175 None:intergenic
ACGGAATGGAAATTGTGGGG+TGG 0.687703 2.3:+56236726 None:intergenic
GGCACGGAATGGAAATTGTG+GGG 0.705646 2.3:+56236723 None:intergenic
CTTGGAGAGAGGATACACCG+TGG 0.810527 2.3:-56234847 MS.gene56015:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! AAAAAATTAAATAAGTCAAA+CGG - chr2.3:56234895-56234914 MS.gene56015:CDS 10.0%
!!! ATAATGAGAATTTTTTCTTT+TGG + chr2.3:56236062-56236081 None:intergenic 15.0%
!! ATAAAATAAGAGAGATTTGA+AGG + chr2.3:56235135-56235154 None:intergenic 20.0%
!! ATGAAATTCTTTGAATCTTT+AGG - chr2.3:56236140-56236159 MS.gene56015:intron 20.0%
!! ATTATAGTATTACTGAAACT+CGG - chr2.3:56235169-56235188 MS.gene56015:CDS 20.0%
!! TAAAATAAGAGAGATTTGAA+GGG + chr2.3:56235134-56235153 None:intergenic 20.0%
!! TAAAGATTCAAAGAATTTCA+TGG + chr2.3:56236141-56236160 None:intergenic 20.0%
!!! ATTTTAATATGAAATAGAGG+AGG - chr2.3:56234699-56234718 MS.gene56015:CDS 20.0%
!!! CACATTTTAATATGAAATAG+AGG - chr2.3:56234696-56234715 MS.gene56015:CDS 20.0%
!!! GATAAGTTGAAAACAATTTA+TGG - chr2.3:56234974-56234993 MS.gene56015:CDS 20.0%
!!! TAATAACTTTCTTTTTGCAT+TGG - chr2.3:56235622-56235641 MS.gene56015:CDS 20.0%
!!! TTTTAATATGAAATAGAGGA+GGG - chr2.3:56234700-56234719 MS.gene56015:CDS 20.0%
! AAACTGTATCCAAAAAATTG+AGG + chr2.3:56236614-56236633 None:intergenic 25.0%
! AACTGTATCCAAAAAATTGA+GGG + chr2.3:56236613-56236632 None:intergenic 25.0%
! AATGTTAGTAATTAGCTGAT+AGG + chr2.3:56234807-56234826 None:intergenic 25.0%
! TCTCATTGACATAATAATCA+GGG - chr2.3:56235661-56235680 MS.gene56015:intron 25.0%
!! TAATATGAAGATTTTGCATC+AGG - chr2.3:56235202-56235221 MS.gene56015:CDS 25.0%
!! TTCAATTGTCCACAAAATTT+TGG - chr2.3:56236398-56236417 MS.gene56015:intron 25.0%
!!! GTTAAATTCCCTCAATTTTT+TGG - chr2.3:56236602-56236621 MS.gene56015:intron 25.0%
AAGAAAGAGATTGTACCTTA+TGG + chr2.3:56234630-56234649 None:intergenic 30.0%
ACACTGTAACTAACAATAGT+TGG + chr2.3:56236311-56236330 None:intergenic 30.0%
AGATTCAAAGAATTTCATGG+TGG + chr2.3:56236138-56236157 None:intergenic 30.0%
ATAAGCATTAGACCTTTCAT+TGG - chr2.3:56235347-56235366 MS.gene56015:CDS 30.0%
ATTCGTTTGAGCTTATCAAT+TGG + chr2.3:56235055-56235074 None:intergenic 30.0%
CACTGTAACTAACAATAGTT+GGG + chr2.3:56236310-56236329 None:intergenic 30.0%
CTCCAAGAAAAATTGTGTTA+CGG + chr2.3:56236450-56236469 None:intergenic 30.0%
CTTATCTTCATAAGCTATCT+AGG + chr2.3:56234960-56234979 None:intergenic 30.0%
GAATGAATATTGACCAGATT+AGG - chr2.3:56235946-56235965 MS.gene56015:intron 30.0%
GATTCAAAGAATTTCATGGT+GGG + chr2.3:56236137-56236156 None:intergenic 30.0%
GGGATTATGATTATGAGATA+TGG - chr2.3:56236494-56236513 MS.gene56015:intron 30.0%
GTCAAAAAGTAATCCCATTT+CGG + chr2.3:56236557-56236576 None:intergenic 30.0%
GTCTCATTGACATAATAATC+AGG - chr2.3:56235660-56235679 MS.gene56015:intron 30.0%
TATTGTTAGTTACAGTGTAG+AGG - chr2.3:56236313-56236332 MS.gene56015:intron 30.0%
TCCAAGAAAAATTGTGTTAC+GGG + chr2.3:56236449-56236468 None:intergenic 30.0%
TTCACTCTAAACACAAAACT+TGG - chr2.3:56235558-56235577 MS.gene56015:CDS 30.0%
TTCTCATTATCCTCACTAAA+AGG - chr2.3:56236072-56236091 MS.gene56015:CDS 30.0%
! AATTTAACCAGCTCAGATTT+TGG + chr2.3:56236590-56236609 None:intergenic 30.0%
! ATGCTAAGACCAAAATTTTG+TGG + chr2.3:56236410-56236429 None:intergenic 30.0%
! ATGGAAGTGTAAGAGAAAAA+TGG + chr2.3:56234611-56234630 None:intergenic 30.0%
! ATTTAACCAGCTCAGATTTT+GGG + chr2.3:56236589-56236608 None:intergenic 30.0%
! TTCTTTTCTCAGGAAAAATC+TGG - chr2.3:56235252-56235271 MS.gene56015:CDS 30.0%
! TTTTCTCTTACACTTCCATA+AGG - chr2.3:56234612-56234631 MS.gene56015:CDS 30.0%
!!! AAATTTTGGTCTTAGCATTG+AGG - chr2.3:56236412-56236431 MS.gene56015:intron 30.0%
!!! ATGGGATTACTTTTTGACTA+TGG - chr2.3:56236559-56236578 MS.gene56015:intron 30.0%
!!! GTTGAGTTTTTTCTTGAACT+TGG - chr2.3:56236032-56236051 MS.gene56015:intron 30.0%
AAACAAGGTTAATACAGCCT+TGG + chr2.3:56235453-56235472 None:intergenic 35.0%
AAACTATAGTAGGCAAAAGC+TGG + chr2.3:56235990-56236009 None:intergenic 35.0%
AACTATAGTAGGCAAAAGCT+GGG + chr2.3:56235989-56236008 None:intergenic 35.0%
AATATGAAATAGAGGAGGGT+TGG - chr2.3:56234704-56234723 MS.gene56015:CDS 35.0%
ACCTCGTTTCTTCAAAAACA+AGG + chr2.3:56235468-56235487 None:intergenic 35.0%
ACTAAAAGGCCTCAATTATG+TGG - chr2.3:56236086-56236105 MS.gene56015:CDS 35.0%
AGTGTAAGAGAAAAATGGCA+CGG + chr2.3:56234606-56234625 None:intergenic 35.0%
ATATGAAATAGAGGAGGGTT+GGG - chr2.3:56234705-56234724 MS.gene56015:CDS 35.0%
ATTGCTTATCTGATGGAACT+CGG - chr2.3:56235924-56235943 MS.gene56015:CDS 35.0%
CATAATCCCAATTTCTTCCA+CGG + chr2.3:56236483-56236502 None:intergenic 35.0%
GAGAGAAGGTGATCAATAAA+AGG + chr2.3:56235533-56235552 None:intergenic 35.0%
GGTTGCATGCTATTCATATA+AGG + chr2.3:56235092-56235111 None:intergenic 35.0%
GTGGACAATTGAATAGAAGA+AGG + chr2.3:56236391-56236410 None:intergenic 35.0%
TAGATGTTGCAATTGACATG+AGG - chr2.3:56235301-56235320 MS.gene56015:CDS 35.0%
TCAAGAAAAAACTCAACCAC+TGG + chr2.3:56236029-56236048 None:intergenic 35.0%
TCCACCTGTTAAGGATAAAT+CGG - chr2.3:56235791-56235810 MS.gene56015:CDS 35.0%
TCCATCAGATAAGCAATATG+AGG + chr2.3:56235921-56235940 None:intergenic 35.0%
TCCTCATATTGCTTATCTGA+TGG - chr2.3:56235917-56235936 MS.gene56015:CDS 35.0%
TGAGAATTTGATGCCGAAAT+GGG - chr2.3:56236541-56236560 MS.gene56015:intron 35.0%
TTTACCCTCCAAACTATAGT+AGG + chr2.3:56236000-56236019 None:intergenic 35.0%
TTTGCCTACTATAGTTTGGA+GGG - chr2.3:56235993-56236012 MS.gene56015:intron 35.0%
! ACACAATTTTTCTTGGAGAG+AGG - chr2.3:56236452-56236471 MS.gene56015:intron 35.0%
! AGAAATTCTAGTGGATGTAG+TGG - chr2.3:56235755-56235774 MS.gene56015:CDS 35.0%
! AGACTGATTCCACATAATTG+AGG + chr2.3:56236098-56236117 None:intergenic 35.0%
! AGCTTTTGCCTACTATAGTT+TGG - chr2.3:56235989-56236008 MS.gene56015:intron 35.0%
! ATAATTGAGGCCTTTTAGTG+AGG + chr2.3:56236085-56236104 None:intergenic 35.0%
! CTAATCACCAGAGTTTTACT+CGG + chr2.3:56234774-56234793 None:intergenic 35.0%
! GGAGAGGAAAAAAAGCATAA+TGG + chr2.3:56235113-56235132 None:intergenic 35.0%
! TTATCCTTAACAGGTGGATT+TGG + chr2.3:56235789-56235808 None:intergenic 35.0%
! TTCCTTTTCTGCTGCTAATA+GGG + chr2.3:56235426-56235445 None:intergenic 35.0%
! TTGTGTTACGGGCTTTATAT+TGG + chr2.3:56236438-56236457 None:intergenic 35.0%
! TTTTGCCTACTATAGTTTGG+AGG - chr2.3:56235992-56236011 MS.gene56015:intron 35.0%
!! TGAATCTTTAGGTGTTGACA+AGG - chr2.3:56236151-56236170 MS.gene56015:intron 35.0%
!!! ACCTTGTTTTTGAAGAAACG+AGG - chr2.3:56235464-56235483 MS.gene56015:CDS 35.0%
AACCCTATTAGCAGCAGAAA+AGG - chr2.3:56235421-56235440 MS.gene56015:CDS 40.0%
AAGAGAAAAATGGCACGGAA+TGG + chr2.3:56234601-56234620 None:intergenic 40.0%
AATGTACTTCCGTTCTCTAG+GGG - chr2.3:56236355-56236374 MS.gene56015:intron 40.0%
AGAAAAGAAGACCCTTGTAG+AGG + chr2.3:56235241-56235260 None:intergenic 40.0%
AGAGCATGGAGAAATTCTAG+TGG - chr2.3:56235746-56235765 MS.gene56015:CDS 40.0%
AGCTTACAACTCTTGAGATC+AGG - chr2.3:56235688-56235707 MS.gene56015:intron 40.0%
ATGCACGCGTGAAAAATTCT+GGG - chr2.3:56236657-56236676 MS.gene56015:intron 40.0%
CAATGTACTTCCGTTCTCTA+GGG - chr2.3:56236354-56236373 MS.gene56015:intron 40.0%
CACAAAACTTGGTACCTTTC+AGG - chr2.3:56235569-56235588 MS.gene56015:CDS 40.0%
CTATCCAAATCCACCTGTTA+AGG - chr2.3:56235782-56235801 MS.gene56015:CDS 40.0%
CTGAGAATTTGATGCCGAAA+TGG - chr2.3:56236540-56236559 MS.gene56015:intron 40.0%
GAATCACTTTACGAAGAGCA+TGG - chr2.3:56235732-56235751 MS.gene56015:intron 40.0%
GATACACCGTGGAAGAAATT+GGG - chr2.3:56236474-56236493 MS.gene56015:intron 40.0%
GCTTACAACTCTTGAGATCA+GGG - chr2.3:56235689-56235708 MS.gene56015:intron 40.0%
GGTGAGATTACTGTTGAATC+AGG - chr2.3:56236679-56236698 MS.gene56015:intron 40.0%
GTCGCATAATAGTCCTAATC+TGG + chr2.3:56235962-56235981 None:intergenic 40.0%
TAAGAGAGATTTGAAGGGAG+AGG + chr2.3:56235129-56235148 None:intergenic 40.0%
TATGCACGCGTGAAAAATTC+TGG - chr2.3:56236656-56236675 MS.gene56015:intron 40.0%
TCAACTTCCTTCCTCTACAA+GGG - chr2.3:56235227-56235246 MS.gene56015:CDS 40.0%
TCAATGAGACTGTGATTAGC+AGG + chr2.3:56235650-56235669 None:intergenic 40.0%
TCCGATTTATCCTTAACAGG+TGG + chr2.3:56235795-56235814 None:intergenic 40.0%
TTGTAGCAACCAAACCTGAA+AGG + chr2.3:56235586-56235605 None:intergenic 40.0%
! CTTCCTTTTCTGCTGCTAAT+AGG + chr2.3:56235427-56235446 None:intergenic 40.0%
! GAGTACAAAGCACCAATGAA+AGG + chr2.3:56235362-56235381 None:intergenic 40.0%
! GCCCGTAACACAATTTTTCT+TGG - chr2.3:56236445-56236464 MS.gene56015:intron 40.0%
! GTAGATTTTCTCCGCGAATT+TGG - chr2.3:56236245-56236264 MS.gene56015:intron 40.0%
! TACAAGGGTCTTCTTTTCTC+AGG - chr2.3:56235242-56235261 MS.gene56015:CDS 40.0%
! TTTTCTGCTGCTAATAGGGT+TGG + chr2.3:56235422-56235441 None:intergenic 40.0%
! TTTTTCACGCGTGCATATCT+CGG + chr2.3:56236653-56236672 None:intergenic 40.0%
!! AACTTGGTACCTTTCAGGTT+TGG - chr2.3:56235574-56235593 MS.gene56015:CDS 40.0%
!! AGGTGTTGACAAGGAACAAT+GGG - chr2.3:56236160-56236179 MS.gene56015:intron 40.0%
!! CTGTCCAAAAAAAGTGCTTC+AGG - chr2.3:56234830-56234849 MS.gene56015:CDS 40.0%
!! TAGGTGTTGACAAGGAACAA+TGG - chr2.3:56236159-56236178 MS.gene56015:intron 40.0%
!!! CTGAAGCACTTTTTTTGGAC+AGG + chr2.3:56234832-56234851 None:intergenic 40.0%
AAGAAGACCCTTGTAGAGGA+AGG + chr2.3:56235237-56235256 None:intergenic 45.0%
ACTTTCTTAGAGTCTACCGC+TGG + chr2.3:56235846-56235865 None:intergenic 45.0%
AGAGGATCCGAGTAAAACTC+TGG - chr2.3:56234764-56234783 MS.gene56015:CDS 45.0%
AGCAGAAAAGGAAGAAGCCA+AGG - chr2.3:56235433-56235452 MS.gene56015:CDS 45.0%
ATGGCACGGAATGGAAATTG+TGG + chr2.3:56234592-56234611 None:intergenic 45.0%
ATTGTGGGGTGGTTGAATTG+AGG + chr2.3:56234576-56234595 None:intergenic 45.0%
CAACATCAACCCCTAGAGAA+CGG + chr2.3:56236367-56236386 None:intergenic 45.0%
CTCAACTTCCTTCCTCTACA+AGG - chr2.3:56235226-56235245 MS.gene56015:CDS 45.0%
CTGCGTGCTATAAGTTAGCA+GGG + chr2.3:56236210-56236229 None:intergenic 45.0%
GCAATGTACTTCCGTTCTCT+AGG - chr2.3:56236353-56236372 MS.gene56015:intron 45.0%
GCGTCCGATTTATCCTTAAC+AGG + chr2.3:56235798-56235817 None:intergenic 45.0%
GGAGGGTAAAATTAAGCCAG+TGG - chr2.3:56236010-56236029 MS.gene56015:intron 45.0%
GGATACACCGTGGAAGAAAT+TGG - chr2.3:56236473-56236492 MS.gene56015:intron 45.0%
GGATTACCCAAAATCTGAGC+TGG - chr2.3:56236580-56236599 MS.gene56015:intron 45.0%
GGTGTCTCAGAAGAGAACAT+AGG - chr2.3:56236266-56236285 MS.gene56015:intron 45.0%
TAGAGTGAAGCTTCGAGAGA+AGG + chr2.3:56235547-56235566 None:intergenic 45.0%
TATACCACTCACTCGAGACA+CGG + chr2.3:56235874-56235893 None:intergenic 45.0%
TCAAGGGTTGAAGCAGTTAG+AGG - chr2.3:56234746-56234765 MS.gene56015:CDS 45.0%
TCTGCGTGCTATAAGTTAGC+AGG + chr2.3:56236211-56236230 None:intergenic 45.0%
TGCACGCGTGAAAAATTCTG+GGG - chr2.3:56236658-56236677 MS.gene56015:intron 45.0%
TGCTATAAGTTAGCAGGGCT+GGG + chr2.3:56236205-56236224 None:intergenic 45.0%
TGGCACGGAATGGAAATTGT+GGG + chr2.3:56234591-56234610 None:intergenic 45.0%
! GAACGGAAGTACATTGCTGT+CGG + chr2.3:56236350-56236369 None:intergenic 45.0%
!! AGCTCAACTGGTTTGAGTCA+AGG - chr2.3:56234729-56234748 MS.gene56015:CDS 45.0%
!! CTTCTGAGACACCAAATTCG+CGG + chr2.3:56236259-56236278 None:intergenic 45.0%
!! GCTCAACTGGTTTGAGTCAA+GGG - chr2.3:56234730-56234749 MS.gene56015:CDS 45.0%
!!! AGCGCCTGAAGCACTTTTTT+TGG + chr2.3:56234837-56234856 None:intergenic 45.0%
AAGTTAGCAGGGCTGGGAAA+CGG + chr2.3:56236199-56236218 None:intergenic 50.0%
ACGGAATGGAAATTGTGGGG+TGG + chr2.3:56234587-56234606 None:intergenic 50.0%
AGTGGTATAGACCCTGCTGA+GGG - chr2.3:56235885-56235904 MS.gene56015:CDS 50.0%
CTAGGACTAGGAGGTGGAAT+TGG + chr2.3:56235822-56235841 None:intergenic 50.0%
GACGAAGATTTCCCTCAGCA+GGG + chr2.3:56235899-56235918 None:intergenic 50.0%
GGCACGGAATGGAAATTGTG+GGG + chr2.3:56234590-56234609 None:intergenic 50.0%
GTGCTATAAGTTAGCAGGGC+TGG + chr2.3:56236206-56236225 None:intergenic 50.0%
TCTGCTGCTAATAGGGTTGG+AGG + chr2.3:56235419-56235438 None:intergenic 50.0%
TTAGAGTCTACCGCTGGACT+AGG + chr2.3:56235840-56235859 None:intergenic 50.0%
!! CGTTCTCTAGGGGTTGATGT+TGG - chr2.3:56236365-56236384 MS.gene56015:intron 50.0%
AAAGCCGTGTCTCGAGTGAG+TGG - chr2.3:56235867-56235886 MS.gene56015:CDS 55.0%
ACCTCCTAGTCCTAGTCCAG+CGG - chr2.3:56235827-56235846 MS.gene56015:CDS 55.0%
CTTGGAGAGAGGATACACCG+TGG - chr2.3:56236463-56236482 MS.gene56015:intron 55.0%
GAGTGGTATAGACCCTGCTG+AGG - chr2.3:56235884-56235903 MS.gene56015:CDS 55.0%
GGACGAAGATTTCCCTCAGC+AGG + chr2.3:56235900-56235919 None:intergenic 55.0%
! TCTACCGCTGGACTAGGACT+AGG + chr2.3:56235834-56235853 None:intergenic 55.0%
GGAGGGTTGGGTAGCTCAAC+TGG - chr2.3:56234717-56234736 MS.gene56015:CDS 60.0%
! ACCGCTGGACTAGGACTAGG+AGG + chr2.3:56235831-56235850 None:intergenic 60.0%
! GCTGGACTAGGACTAGGAGG+TGG + chr2.3:56235828-56235847 None:intergenic 60.0%


Chromosome Type Strat End Strand Name
chr2.3 gene 56234560 56236772 56234560 ID=MS.gene56015
chr2.3 mRNA 56234560 56236772 56234560 ID=MS.gene56015.t1;Parent=MS.gene56015
chr2.3 exon 56236699 56236772 56236699 ID=MS.gene56015.t1.exon1;Parent=MS.gene56015.t1
chr2.3 CDS 56236699 56236772 56236699 ID=cds.MS.gene56015.t1;Parent=MS.gene56015.t1
chr2.3 exon 56236059 56236120 56236059 ID=MS.gene56015.t1.exon2;Parent=MS.gene56015.t1
chr2.3 CDS 56236059 56236120 56236059 ID=cds.MS.gene56015.t1;Parent=MS.gene56015.t1
chr2.3 exon 56235742 56235948 56235742 ID=MS.gene56015.t1.exon3;Parent=MS.gene56015.t1
chr2.3 CDS 56235742 56235948 56235742 ID=cds.MS.gene56015.t1;Parent=MS.gene56015.t1
chr2.3 exon 56234560 56235650 56234560 ID=MS.gene56015.t1.exon4;Parent=MS.gene56015.t1
chr2.3 CDS 56234560 56235650 56234560 ID=cds.MS.gene56015.t1;Parent=MS.gene56015.t1
Gene Sequence

>MS.gene56015

ATGAAAACTTTCTCCTCAATTCAACCACCCCACAATTTCCATTCCGTGCCATTTTTCTCTTACACTTCCATAAGGTACAATCTCTTTCTTATTTTCATGTATCAATGTCTTTTTTTTTTTTTTTCTTTTCATTATACACATTTTAATATGAAATAGAGGAGGGTTGGGTAGCTCAACTGGTTTGAGTCAAGGGTTGAAGCAGTTAGAGGATCCGAGTAAAACTCTGGTGATTAGAGAATACTAACCTATCAGCTAATTACTAACATTTGCCTGTCCAAAAAAAGTGCTTCAGGCGCTTATCATATAAGTGCTTATATGTAAGCTATTTCTGTAACAAAAAATTAAATAAGTCAAACGGTTTTTATATATCTTATAAGTTATTTTTTTCGTAAGTTATCCTAGATAGCTTATGAAGATAAGTTGAAAACAATTTATGGACATGTCATAATGTGTTTTCGTAAGCACTCTCAAACAGTCTTACAAGTGTTTATGCCAATTGATAAGCTCAAACGAATCAATTCAAGCAGAGCCTTATATGAATAGCATGCAACCATTATGCTTTTTTTCCTCTCCCTTCAAATCTCTCTTATTTTATCTTCTATATTTATGATTATAGTATTACTGAAACTCGGATTTTTTCTCTAATATGAAGATTTTGCATCAGGACTCAACTTCCTTCCTCTACAAGGGTCTTCTTTTCTCAGGAAAAATCTGGTATGCAATCTTGTTCAAAGCTTCATTTAGATGTTGCAATTGACATGAGGTTATTTAATAATGTACTATTCTAATAAGCATTAGACCTTTCATTGGTGCTTTGTACTCAGACACAGATGAGTCATTAAACGTAAAACTGTTGCCTCCAACCCTATTAGCAGCAGAAAAGGAAGAAGCCAAGGCTGTATTAACCTTGTTTTTGAAGAAACGAGGTTTGAGCAATGCAAATGCAGCAAGAACGATTAACAAGTCAGATCCTTTTATTGATCACCTTCTCTCGAAGCTTCACTCTAAACACAAAACTTGGTACCTTTCAGGTTTGGTTGCTACAAATTAATCACTTCTATTTAATAACTTTCTTTTTGCATTGGATCCTGCTAATCACAGTCTCATTGACATAATAATCAGGGAGAGAGCTTACAACTCTTGAGATCAGGGATGCTCTTATCTCTTATCTCGAATCACTTTACGAAGAGCATGGAGAAATTCTAGTGGATGTAGTGGAAAACTATCCAAATCCACCTGTTAAGGATAAATCGGACGCACCAATTCCACCTCCTAGTCCTAGTCCAGCGGTAGACTCTAAGAAAGTTAAAGCCGTGTCTCGAGTGAGTGGTATAGACCCTGCTGAGGGAAATCTTCGTCCTCATATTGCTTATCTGATGGAACTCGGAATGAATATTGACCAGATTAGGACTATTATGCGACGATTCCCAGCTTTTGCCTACTATAGTTTGGAGGGTAAAATTAAGCCAGTGGTTGAGTTTTTTCTTGAACTTGGAGTACCAAAAGAAAAAATTCTCATTATCCTCACTAAAAGGCCTCAATTATGTGGAATCAGTCTATCTAAAAATCTTAAGCCCACCATGAAATTCTTTGAATCTTTAGGTGTTGACAAGGAACAATGGGCGAAAGTGATCTACCGTTTCCCAGCCCTGCTAACTTATAGCACGCAGAAGATTAATGAAAGTGTAGATTTTCTCCGCGAATTTGGTGTCTCAGAAGAGAACATAGGTAAGATTTTAACTCGCTGCCCAACTATTGTTAGTTACAGTGTAGAGGACAATCTTCGACCGACAGCAATGTACTTCCGTTCTCTAGGGGTTGATGTTGGCCTTCTTCTATTCAATTGTCCACAAAATTTTGGTCTTAGCATTGAGGCCAATATAAAGCCCGTAACACAATTTTTCTTGGAGAGAGGATACACCGTGGAAGAAATTGGGATTATGATTATGAGATATGGAATGCTGTACACATTCAGCTTGACTGAGAATTTGATGCCGAAATGGGATTACTTTTTGACTATGGATTACCCAAAATCTGAGCTGGTTAAATTCCCTCAATTTTTTGGATACAGTTTAGAGCAAAGAATTAAACCGAGATATGCACGCGTGAAAAATTCTGGGGTGAGATTACTGTTGAATCAGGTTCTTTCACTGTCAAGCAGCAACTTTGACGAGATCTTAAGAAAGAAAATAAAGAAAATGCAAGTTGATTAG
Protein sequence

>MS.gene56015.t1

MKTFSSIQPPHNFHSVPFFSYTSIRFCIRTQLPSSTRVFFSQEKSDTDESLNVKLLPPTLLAAEKEEAKAVLTLFLKKRGLSNANAARTINKSDPFIDHLLSKLHSKHKTWYLSGRELTTLEIRDALISYLESLYEEHGEILVDVVENYPNPPVKDKSDAPIPPPSPSPAVDSKKVKAVSRVSGIDPAEGNLRPHIAYLMELGMNIDQIRTIMRRFPAFAYYSLEGKIKPVVEFFLELGVPKEKILIILTKRPQLCGISLSKNLKPTMKFFESLGVDKEQWAKVIYRFPALLTYSTQKINESVDFLREFGVSEENIGKILTRCPTIVSYSVEDNLRPTAMYFRSLGVDVGLLLFNCPQNFGLSIEANIKPVTQFFLERGYTVEEIGIMIMRYGMLYTFSLTENLMPKWDYFLTMDYPKSELVKFPQFFGYSLEQRIKPRYARVKNSGVRLLLNQVLSLSSSNFDEILRKKIKKMQVD