Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene56126.t1 | AES77968.1 | 98.9 | 188 | 2 | 0 | 1 | 188 | 1 | 188 | 2.90E-98 | 367.9 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene56126.t1 | P50346 | 68.3 | 104 | 32 | 1 | 7 | 110 | 11 | 113 | 6.9e-32 | 138.7 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene56126.t1 | G7KUS1 | 98.9 | 188 | 2 | 0 | 1 | 188 | 1 | 188 | 2.1e-98 | 367.9 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene053224 | MS.gene56126 | 0.801207 | 1.00E-48 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene56126.t1 | MTR_7g022400 | 98.936 | 188 | 2 | 0 | 1 | 188 | 1 | 188 | 1.15e-139 | 387 |
| MS.gene56126.t1 | MTR_7g112750 | 81.818 | 187 | 16 | 1 | 1 | 187 | 1 | 169 | 1.79e-107 | 306 |
| MS.gene56126.t1 | MTR_3g108280 | 67.308 | 104 | 33 | 1 | 7 | 110 | 11 | 113 | 5.54e-41 | 141 |
| MS.gene56126.t1 | MTR_5g082180 | 66.346 | 104 | 34 | 1 | 7 | 110 | 11 | 113 | 2.06e-40 | 139 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene56126.t1 | AT2G40010 | 61.404 | 114 | 39 | 2 | 1 | 110 | 1 | 113 | 5.27e-38 | 133 |
| MS.gene56126.t1 | AT3G09200 | 63.462 | 104 | 37 | 1 | 7 | 110 | 10 | 112 | 7.73e-38 | 132 |
| MS.gene56126.t1 | AT3G11250 | 62.500 | 104 | 38 | 1 | 7 | 110 | 10 | 112 | 3.25e-37 | 131 |
| MS.gene56126.t1 | AT3G09200 | 42.308 | 104 | 26 | 2 | 7 | 110 | 10 | 79 | 1.82e-14 | 70.1 |
Find 48 sgRNAs with CRISPR-Local
Find 51 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| TGTTGGATCGAACCAGCTTC+AGG | 0.326070 | 7.4:-79547069 | MS.gene56126:CDS |
| CGAACCAGCTTCAGGGTATA+AGG | 0.338571 | 7.4:-79547061 | MS.gene56126:CDS |
| AGTTCTTGTTGACAAGTATA+TGG | 0.346686 | 7.4:-79546650 | MS.gene56126:CDS |
| TCGAGAGTACACACAAGTTT+TGG | 0.357954 | 7.4:-79547108 | MS.gene56126:CDS |
| ACCCAAGCAGCGGAGAAAAC+TGG | 0.386159 | 7.4:-79546975 | MS.gene56126:CDS |
| CGGAAGCTTGTGAAGATTGT+TGG | 0.393329 | 7.4:+79546736 | None:intergenic |
| CAAGGAGTGGAACGAGGTTA+AGG | 0.407384 | 7.4:+79546937 | None:intergenic |
| ATCTCATCATGTAACAGTGC+TGG | 0.426008 | 7.4:+79546679 | None:intergenic |
| AACCTCGTTCCACTCCTTGT+GGG | 0.430936 | 7.4:-79546933 | MS.gene56126:CDS |
| ATTTGGATCGGTAACATGGC+TGG | 0.436230 | 7.4:-79547176 | None:intergenic |
| GGCAGCTTACGACGCGAAGA+TGG | 0.466083 | 7.4:-79547141 | MS.gene56126:CDS |
| AACATCCCTCAAGTCACCTT+TGG | 0.471136 | 7.4:+79546890 | None:intergenic |
| GCAATCCGGACAGTTGCCAT+TGG | 0.473141 | 7.4:-79546775 | MS.gene56126:CDS |
| AGTCATATGACATGTACTTC+GGG | 0.478273 | 7.4:+79546820 | None:intergenic |
| TTACCAGCCAAAAGGAGAAA+AGG | 0.483227 | 7.4:+79546624 | None:intergenic |
| CGGTAACATGGCTGGGAAGG+TGG | 0.498093 | 7.4:-79547168 | None:intergenic |
| CAATCCGGACAGTTGCCATT+GGG | 0.503959 | 7.4:-79546774 | MS.gene56126:CDS |
| GTGGTTTCATCAGACAATGT+TGG | 0.507329 | 7.4:-79547086 | MS.gene56126:CDS |
| CTCTCCTTATACCCTGAAGC+TGG | 0.512942 | 7.4:+79547057 | None:intergenic |
| TAACCTCGTTCCACTCCTTG+TGG | 0.515777 | 7.4:-79546934 | MS.gene56126:CDS |
| GAGGCCCAATGGCAACTGTC+CGG | 0.527511 | 7.4:+79546770 | None:intergenic |
| GATCGGTAACATGGCTGGGA+AGG | 0.528105 | 7.4:-79547171 | None:intergenic |
| TACTTCGGGCACATGAGAAT+AGG | 0.529103 | 7.4:+79546834 | None:intergenic |
| TCTCATCATGTAACAGTGCT+GGG | 0.529636 | 7.4:+79546680 | None:intergenic |
| ACCTCGTTCCACTCCTTGTG+GGG | 0.531150 | 7.4:-79546932 | MS.gene56126:CDS |
| GTTGGATCGAACCAGCTTCA+GGG | 0.531277 | 7.4:-79547068 | MS.gene56126:CDS |
| ATCACAAATTACCAGCCAAA+AGG | 0.540624 | 7.4:+79546616 | None:intergenic |
| GAAGATTCAGTGGTTGTGAT+GGG | 0.554294 | 7.4:-79547026 | MS.gene56126:CDS |
| CATGGCTGGGAAGGTGGCGA+AGG | 0.555229 | 7.4:-79547162 | MS.gene56126:CDS |
| TTTGGATCGGTAACATGGCT+GGG | 0.555330 | 7.4:-79547175 | None:intergenic |
| GAGTCATATGACATGTACTT+CGG | 0.556970 | 7.4:+79546819 | None:intergenic |
| GCAGCTTACGACGCGAAGAT+GGG | 0.559567 | 7.4:-79547140 | MS.gene56126:CDS |
| ACTTGTTCCTACATGCAATC+CGG | 0.559664 | 7.4:-79546789 | MS.gene56126:CDS |
| TGAGCTTACAAGAAAAGGCT+CGG | 0.573435 | 7.4:+79546716 | None:intergenic |
| TGAACGAATTGCTAAGCTCA+AGG | 0.576611 | 7.4:-79546865 | MS.gene56126:CDS |
| GCAACGTTCCCCACAAGGAG+TGG | 0.578891 | 7.4:+79546924 | None:intergenic |
| TGAAGATTCAGTGGTTGTGA+TGG | 0.591106 | 7.4:-79547027 | MS.gene56126:CDS |
| CAACTGTCCGGATTGCATGT+AGG | 0.593652 | 7.4:+79546782 | None:intergenic |
| ATCATGTAACAGTGCTGGGG+TGG | 0.595286 | 7.4:+79546684 | None:intergenic |
| TTACAACATGTGAGGCCCAA+TGG | 0.599780 | 7.4:+79546759 | None:intergenic |
| TCAAAGCAACGTTCCCCACA+AGG | 0.602062 | 7.4:+79546919 | None:intergenic |
| GAACGAGGTTAAGGAAAGCA+TGG | 0.617046 | 7.4:+79546946 | None:intergenic |
| AGCACTGCATGAAGATTCAG+TGG | 0.637836 | 7.4:-79547036 | MS.gene56126:CDS |
| ATTGTTGGTTACAACATGTG+AGG | 0.653243 | 7.4:+79546751 | None:intergenic |
| CTCATCATGTAACAGTGCTG+GGG | 0.655453 | 7.4:+79546681 | None:intergenic |
| ACGCTCTATTACCCAAGCAG+CGG | 0.673282 | 7.4:-79546985 | MS.gene56126:CDS |
| TCCCCACAAGGAGTGGAACG+AGG | 0.691919 | 7.4:+79546931 | None:intergenic |
| AAGATTCAGTGGTTGTGATG+GGG | 0.703344 | 7.4:-79547025 | MS.gene56126:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | AATTTTGAGCTTACAAGAAA+AGG | + | chr7.4:79547070-79547089 | None:intergenic | 25.0% |
| AGTTCTTGTTGACAAGTATA+TGG | - | chr7.4:79547128-79547147 | MS.gene56126:CDS | 30.0% | |
| !! | GTTGCTTTGATTTTTACCAA+AGG | - | chr7.4:79546872-79546891 | MS.gene56126:CDS | 30.0% |
| AGTCATATGACATGTACTTC+GGG | + | chr7.4:79546961-79546980 | None:intergenic | 35.0% | |
| GAGTCATATGACATGTACTT+CGG | + | chr7.4:79546962-79546981 | None:intergenic | 35.0% | |
| ! | ATTGTTGGTTACAACATGTG+AGG | + | chr7.4:79547030-79547049 | None:intergenic | 35.0% |
| ! | TTTTACCAAAGGTGACTTGA+GGG | - | chr7.4:79546883-79546902 | MS.gene56126:CDS | 35.0% |
| ! | TTTTTACCAAAGGTGACTTG+AGG | - | chr7.4:79546882-79546901 | MS.gene56126:CDS | 35.0% |
| AAGATTCAGTGGTTGTGATG+GGG | - | chr7.4:79546753-79546772 | MS.gene56126:CDS | 40.0% | |
| ACTTGTTCCTACATGCAATC+CGG | - | chr7.4:79546989-79547008 | MS.gene56126:CDS | 40.0% | |
| ATCTCATCATGTAACAGTGC+TGG | + | chr7.4:79547102-79547121 | None:intergenic | 40.0% | |
| GAAGATTCAGTGGTTGTGAT+GGG | - | chr7.4:79546752-79546771 | MS.gene56126:CDS | 40.0% | |
| GTGGTTTCATCAGACAATGT+TGG | - | chr7.4:79546692-79546711 | MS.gene56126:CDS | 40.0% | |
| TCTCATCATGTAACAGTGCT+GGG | + | chr7.4:79547101-79547120 | None:intergenic | 40.0% | |
| TGAACGAATTGCTAAGCTCA+AGG | - | chr7.4:79546913-79546932 | MS.gene56126:CDS | 40.0% | |
| TGAAGATTCAGTGGTTGTGA+TGG | - | chr7.4:79546751-79546770 | MS.gene56126:CDS | 40.0% | |
| TGAGCTTACAAGAAAAGGCT+CGG | + | chr7.4:79547065-79547084 | None:intergenic | 40.0% | |
| TTACCAGCCAAAAGGAGAAA+AGG | + | chr7.4:79547157-79547176 | None:intergenic | 40.0% | |
| ! | ATGGTCTCCTTTTCTCCTTT+TGG | - | chr7.4:79547147-79547166 | MS.gene56126:CDS | 40.0% |
| !! | AGAGTACACACAAGTTTTGG+TGG | - | chr7.4:79546673-79546692 | MS.gene56126:CDS | 40.0% |
| !! | TCGAGAGTACACACAAGTTT+TGG | - | chr7.4:79546670-79546689 | MS.gene56126:CDS | 40.0% |
| AACATCCCTCAAGTCACCTT+TGG | + | chr7.4:79546891-79546910 | None:intergenic | 45.0% | |
| AGCACTGCATGAAGATTCAG+TGG | - | chr7.4:79546742-79546761 | MS.gene56126:CDS | 45.0% | |
| CGGAAGCTTGTGAAGATTGT+TGG | + | chr7.4:79547045-79547064 | None:intergenic | 45.0% | |
| CTCATCATGTAACAGTGCTG+GGG | + | chr7.4:79547100-79547119 | None:intergenic | 45.0% | |
| GAACGAGGTTAAGGAAAGCA+TGG | + | chr7.4:79546835-79546854 | None:intergenic | 45.0% | |
| TACTTCGGGCACATGAGAAT+AGG | + | chr7.4:79546947-79546966 | None:intergenic | 45.0% | |
| TTACAACATGTGAGGCCCAA+TGG | + | chr7.4:79547022-79547041 | None:intergenic | 45.0% | |
| ! | TCTCCTTTTCTCCTTTTGGC+TGG | - | chr7.4:79547151-79547170 | MS.gene56126:CDS | 45.0% |
| AACCTCGTTCCACTCCTTGT+GGG | - | chr7.4:79546845-79546864 | MS.gene56126:CDS | 50.0% | |
| ACGCTCTATTACCCAAGCAG+CGG | - | chr7.4:79546793-79546812 | MS.gene56126:CDS | 50.0% | |
| CAACTGTCCGGATTGCATGT+AGG | + | chr7.4:79546999-79547018 | None:intergenic | 50.0% | |
| CAAGGAGTGGAACGAGGTTA+AGG | + | chr7.4:79546844-79546863 | None:intergenic | 50.0% | |
| CAATCCGGACAGTTGCCATT+GGG | - | chr7.4:79547004-79547023 | MS.gene56126:CDS | 50.0% | |
| CGAACCAGCTTCAGGGTATA+AGG | - | chr7.4:79546717-79546736 | MS.gene56126:CDS | 50.0% | |
| CTCTCCTTATACCCTGAAGC+TGG | + | chr7.4:79546724-79546743 | None:intergenic | 50.0% | |
| GTTGGATCGAACCAGCTTCA+GGG | - | chr7.4:79546710-79546729 | MS.gene56126:CDS | 50.0% | |
| TAACCTCGTTCCACTCCTTG+TGG | - | chr7.4:79546844-79546863 | MS.gene56126:CDS | 50.0% | |
| TCAAAGCAACGTTCCCCACA+AGG | + | chr7.4:79546862-79546881 | None:intergenic | 50.0% | |
| TGTTGGATCGAACCAGCTTC+AGG | - | chr7.4:79546709-79546728 | MS.gene56126:CDS | 50.0% | |
| ! | ATCATGTAACAGTGCTGGGG+TGG | + | chr7.4:79547097-79547116 | None:intergenic | 50.0% |
| ! | TCCAGTTTTCTCCGCTGCTT+GGG | + | chr7.4:79546807-79546826 | None:intergenic | 50.0% |
| ! | TTCCAGTTTTCTCCGCTGCT+TGG | + | chr7.4:79546808-79546827 | None:intergenic | 50.0% |
| ACCCAAGCAGCGGAGAAAAC+TGG | - | chr7.4:79546803-79546822 | MS.gene56126:CDS | 55.0% | |
| ACCTCGTTCCACTCCTTGTG+GGG | - | chr7.4:79546846-79546865 | MS.gene56126:CDS | 55.0% | |
| GCAATCCGGACAGTTGCCAT+TGG | - | chr7.4:79547003-79547022 | MS.gene56126:CDS | 55.0% | |
| GCAGCTTACGACGCGAAGAT+GGG | - | chr7.4:79546638-79546657 | MS.gene56126:CDS | 55.0% | |
| GAGGCCCAATGGCAACTGTC+CGG | + | chr7.4:79547011-79547030 | None:intergenic | 60.0% | |
| GCAACGTTCCCCACAAGGAG+TGG | + | chr7.4:79546857-79546876 | None:intergenic | 60.0% | |
| GGCAGCTTACGACGCGAAGA+TGG | - | chr7.4:79546637-79546656 | MS.gene56126:CDS | 60.0% | |
| TCCCCACAAGGAGTGGAACG+AGG | + | chr7.4:79546850-79546869 | None:intergenic | 60.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr7.4 | gene | 79546617 | 79547183 | 79546617 | ID=MS.gene56126 |
| chr7.4 | mRNA | 79546617 | 79547183 | 79546617 | ID=MS.gene56126.t1;Parent=MS.gene56126 |
| chr7.4 | exon | 79546617 | 79547183 | 79546617 | ID=MS.gene56126.t1.exon1;Parent=MS.gene56126.t1 |
| chr7.4 | CDS | 79546617 | 79547183 | 79546617 | ID=cds.MS.gene56126.t1;Parent=MS.gene56126.t1 |
| Gene Sequence |
| Protein sequence |
