AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene56406

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene56406.t1 MTR_4g084480 96.491 399 14 0 1 399 1 399 0.0 788
MS.gene56406.t1 MTR_4g084480 94.026 385 23 0 1 385 1 385 0.0 738
MS.gene56406.t1 MTR_4g084480 96.448 366 13 0 8 373 1 366 0.0 720
MS.gene56406.t1 MTR_2g019345 69.554 404 105 4 1 395 1 395 0.0 551
MS.gene56406.t1 MTR_1g077990 56.535 329 142 1 23 351 22 349 6.51e-137 397
MS.gene56406.t1 MTR_4g052970 52.941 323 152 0 31 353 28 350 1.44e-125 367
MS.gene56406.t1 MTR_2g086530 50.000 338 167 1 13 350 10 345 8.43e-122 358
MS.gene56406.t1 MTR_2g086530 52.351 319 150 1 32 350 8 324 1.32e-121 357
MS.gene56406.t1 MTR_4g010200 48.235 340 175 1 27 365 28 367 5.78e-117 346
MS.gene56406.t1 MTR_4g114850 45.732 328 177 1 27 353 21 348 1.53e-102 308
MS.gene56406.t1 MTR_4g114850 45.732 328 177 1 27 353 21 348 1.60e-102 309
MS.gene56406.t1 MTR_4g114850 45.732 328 177 1 27 353 21 348 1.96e-102 308
MS.gene56406.t1 MTR_5g085580 49.538 325 161 3 29 351 22 345 2.46e-99 303
MS.gene56406.t1 MTR_3g116510 45.299 351 190 2 14 362 9 359 6.66e-99 302
MS.gene56406.t1 MTR_4g069940 45.014 351 191 2 14 362 10 360 4.53e-98 300
MS.gene56406.t1 MTR_1g022160 45.963 322 172 2 32 351 24 345 9.16e-96 293
MS.gene56406.t1 MTR_1g056640 45.706 326 174 3 28 351 34 358 2.41e-95 294
MS.gene56406.t1 MTR_3g080410 45.217 345 181 4 25 362 19 362 2.99e-95 290
MS.gene56406.t1 MTR_1g022160 45.963 322 172 2 32 351 24 345 4.26e-95 293
MS.gene56406.t1 MTR_1g022160 45.963 322 172 2 32 351 24 345 6.37e-95 293
MS.gene56406.t1 MTR_1g022160 45.963 322 172 2 32 351 24 345 6.75e-95 293
MS.gene56406.t1 MTR_1g007810 45.988 324 169 3 26 347 10 329 7.00e-93 283
MS.gene56406.t1 MTR_6g088885 41.828 361 201 3 33 385 28 387 3.18e-88 276
MS.gene56406.t1 MTR_7g053500 42.769 325 180 5 33 352 27 350 2.18e-86 270
MS.gene56406.t1 MTR_7g026340 44.136 324 173 5 29 350 20 337 2.75e-85 267
MS.gene56406.t1 MTR_8g012400 40.923 325 186 5 33 352 30 353 3.91e-81 256
MS.gene56406.t1 MTR_4g083500 39.840 374 214 8 7 374 19 387 8.77e-78 250
MS.gene56406.t1 MTR_4g088155 39.210 329 192 5 30 351 21 348 1.29e-77 247
MS.gene56406.t1 MTR_3g083580 38.658 313 187 3 30 338 24 335 1.09e-76 246
MS.gene56406.t1 MTR_8g085720 36.443 343 208 4 8 346 7 343 1.14e-74 241
MS.gene56406.t1 MTR_4g132280 39.144 327 194 3 32 354 45 370 1.72e-74 241
MS.gene56406.t1 MTR_1g031900 36.649 382 235 5 8 385 1 379 9.08e-74 239
MS.gene56406.t1 MTR_1g031900 36.649 382 235 5 8 385 1 379 1.84e-73 238
MS.gene56406.t1 MTR_8g102340 36.788 386 202 5 8 385 1 352 3.17e-73 236
MS.gene56406.t1 MTR_4g076490 40.123 324 182 6 30 353 95 406 1.99e-72 233
MS.gene56406.t1 MTR_5g078200 36.528 386 229 7 8 385 1 378 5.47e-72 237
MS.gene56406.t1 MTR_3g065460 36.826 334 206 3 10 339 2 334 1.28e-68 225
MS.gene56406.t1 MTR_5g078200 37.644 348 209 5 43 385 1 345 8.07e-68 226
MS.gene56406.t1 MTR_4g076470 38.941 321 183 7 26 346 17 324 1.03e-66 215
MS.gene56406.t1 MTR_4g076440 38.750 320 183 8 29 348 20 326 2.11e-66 214
MS.gene56406.t1 MTR_1g052430 38.973 331 190 7 20 348 22 342 3.17e-66 216
MS.gene56406.t1 MTR_4g076255 39.319 323 183 6 26 348 19 328 4.64e-66 213
MS.gene56406.t1 MTR_7g053500 43.750 256 139 4 101 352 19 273 4.74e-66 215
MS.gene56406.t1 MTR_4g076430 38.580 324 184 8 26 348 17 326 4.93e-66 213
MS.gene56406.t1 MTR_2g034480 38.769 325 189 7 30 352 25 341 2.45e-63 207
MS.gene56406.t1 MTR_3g116510 44.444 225 125 0 138 362 1 225 2.95e-62 203
MS.gene56406.t1 MTR_4g076570 38.629 321 187 7 31 349 21 333 3.66e-62 204
MS.gene56406.t1 MTR_7g081350 34.030 335 204 7 13 346 15 333 4.11e-62 203
MS.gene56406.t1 MTR_4g076500 37.942 311 183 5 31 340 77 378 2.30e-60 200
MS.gene56406.t1 MTR_8g012400 39.927 273 158 5 85 352 2 273 2.42e-60 200
MS.gene56406.t1 MTR_2g034440 37.143 315 186 8 30 341 20 325 1.03e-58 194
MS.gene56406.t1 MTR_2g034470 35.542 332 202 8 30 358 28 350 1.90e-58 194
MS.gene56406.t1 MTR_2g034470 35.294 323 197 8 39 358 2 315 3.33e-55 185
MS.gene56406.t1 MTR_7g081370 31.085 341 210 7 7 346 5 321 8.09e-54 181
MS.gene56406.t1 MTR_1g010120 33.708 356 215 13 6 352 4 347 9.00e-52 180
MS.gene56406.t1 MTR_2g027560 32.132 333 213 9 27 351 23 350 1.22e-47 169
MS.gene56406.t1 MTR_4g112025 31.129 363 226 13 2 353 6 355 1.54e-47 170
MS.gene56406.t1 MTR_6g032820 32.267 344 208 11 11 341 8 339 2.25e-47 167
MS.gene56406.t1 MTR_4g124440 30.899 356 227 13 6 352 4 349 4.73e-46 165
MS.gene56406.t1 MTR_7g085390 32.743 339 208 11 25 352 19 348 8.57e-46 164
MS.gene56406.t1 MTR_8g092070 29.577 355 235 11 13 359 15 362 5.26e-44 161
MS.gene56406.t1 MTR_1g053320 31.118 331 212 11 30 350 28 352 2.30e-42 155
MS.gene56406.t1 MTR_5g081720 34.967 306 184 10 48 347 53 349 1.11e-41 151
MS.gene56406.t1 MTR_3g108600 30.725 345 209 10 14 352 13 333 2.00e-41 152
MS.gene56406.t1 MTR_3g031770 32.635 334 208 10 31 356 26 350 4.47e-41 152
MS.gene56406.t1 MTR_3g095050 29.532 342 224 13 29 362 31 363 2.35e-37 141
MS.gene56406.t1 MTR_4g134280 35.565 239 142 9 119 351 3 235 5.45e-32 124
MS.gene56406.t1 MTR_4g036775 34.694 147 88 4 248 391 80 221 1.68e-19 87.0
MS.gene56406.t1 MTR_4g036680 34.694 147 88 4 248 391 80 221 3.01e-19 86.3
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene56406.t1 AT4G18340 61.082 388 145 1 3 390 5 386 7.63e-175 493
MS.gene56406.t1 AT4G18340 62.534 371 139 0 3 373 5 375 6.99e-174 490
MS.gene56406.t1 AT1G30080 63.514 370 132 1 25 391 29 398 8.67e-171 483
MS.gene56406.t1 AT1G30080 64.589 353 121 3 1 349 1 353 4.45e-168 476
MS.gene56406.t1 AT2G27500 49.582 359 169 3 33 390 30 377 2.95e-123 362
MS.gene56406.t1 AT2G27500 51.170 342 160 2 33 373 30 365 2.35e-121 357
MS.gene56406.t1 AT1G32860 50.155 323 159 1 31 351 28 350 4.18e-120 355
MS.gene56406.t1 AT5G42100 51.692 325 154 2 29 350 24 348 1.10e-115 344
MS.gene56406.t1 AT5G42100 51.692 325 154 2 29 350 24 348 2.59e-115 343
MS.gene56406.t1 AT3G15800 48.348 333 170 2 27 357 38 370 4.62e-114 339
MS.gene56406.t1 AT2G26600 43.895 344 191 2 27 369 29 371 1.02e-105 317
MS.gene56406.t1 AT4G26830 47.175 354 181 4 8 359 1 350 1.59e-104 317
MS.gene56406.t1 AT4G26830 49.240 329 161 4 33 359 30 354 3.11e-103 313
MS.gene56406.t1 AT5G55180 47.164 335 168 4 18 350 19 346 2.57e-100 306
MS.gene56406.t1 AT5G55180 47.164 335 168 4 18 350 19 346 2.87e-100 306
MS.gene56406.t1 AT2G27500 50.512 293 138 2 82 373 1 287 9.30e-100 299
MS.gene56406.t1 AT5G24318 45.820 323 169 3 30 350 28 346 7.00e-97 294
MS.gene56406.t1 AT2G05790 46.273 322 170 3 29 348 22 342 4.99e-96 295
MS.gene56406.t1 AT5G24318 45.646 333 174 4 30 359 28 356 6.75e-96 294
MS.gene56406.t1 AT5G24318 45.646 333 174 4 30 359 28 356 1.04e-95 294
MS.gene56406.t1 AT4G34480 46.012 326 175 1 33 357 27 352 1.47e-93 285
MS.gene56406.t1 AT5G42720 44.444 342 188 2 12 351 6 347 1.81e-93 288
MS.gene56406.t1 AT4G34480 43.085 376 206 4 13 385 12 382 1.10e-91 285
MS.gene56406.t1 AT5G56590 43.614 321 179 2 33 351 27 347 2.73e-90 281
MS.gene56406.t1 AT2G16230 40.833 360 207 2 33 386 26 385 4.63e-90 281
MS.gene56406.t1 AT3G07320 41.231 325 185 5 33 352 26 349 1.40e-84 265
MS.gene56406.t1 AT2G26600 45.221 272 148 1 98 369 7 277 1.67e-84 259
MS.gene56406.t1 AT3G13560 40.741 324 187 3 27 346 21 343 1.09e-80 256
MS.gene56406.t1 AT3G13560 40.741 324 187 3 27 346 21 343 1.09e-80 256
MS.gene56406.t1 AT3G13560 40.741 324 187 3 27 346 21 343 1.09e-80 256
MS.gene56406.t1 AT3G13560 40.741 324 187 3 27 346 21 343 1.09e-80 256
MS.gene56406.t1 AT3G13560 40.741 324 187 3 27 346 21 343 1.09e-80 256
MS.gene56406.t1 AT4G14080 39.144 327 194 4 29 351 39 364 1.37e-77 248
MS.gene56406.t1 AT1G11820 39.329 328 194 3 33 356 45 371 7.45e-77 243
MS.gene56406.t1 AT3G23770 38.739 333 196 5 26 351 31 362 2.21e-76 244
MS.gene56406.t1 AT1G11820 39.628 323 190 3 33 351 45 366 8.17e-76 243
MS.gene56406.t1 AT1G11820 39.937 318 186 3 33 346 45 361 1.18e-75 244
MS.gene56406.t1 AT4G29360 41.925 322 185 2 26 346 21 341 6.22e-75 243
MS.gene56406.t1 AT4G29360 41.925 322 185 2 26 346 31 351 1.04e-74 242
MS.gene56406.t1 AT2G01630 40.432 324 188 3 32 351 23 345 1.49e-74 237
MS.gene56406.t1 AT2G01630 40.432 324 188 3 32 351 23 345 2.99e-74 238
MS.gene56406.t1 AT2G01630 40.432 324 188 3 32 351 23 345 1.18e-73 238
MS.gene56406.t1 AT3G55430 37.027 370 215 10 27 386 24 385 1.44e-72 234
MS.gene56406.t1 AT3G46570 41.212 330 183 8 26 350 21 344 2.91e-69 223
MS.gene56406.t1 AT1G66250 37.027 370 219 7 21 385 26 386 1.33e-68 225
MS.gene56406.t1 AT5G20390 37.101 345 205 8 1 342 1 336 5.56e-67 216
MS.gene56406.t1 AT3G57270 38.871 319 183 8 33 348 28 337 1.53e-65 212
MS.gene56406.t1 AT5G20340 38.700 323 183 7 24 342 35 346 1.34e-63 208
MS.gene56406.t1 AT4G34480 44.238 269 148 2 119 385 3 271 1.63e-63 209
MS.gene56406.t1 AT3G61810 36.364 330 188 6 32 351 55 372 4.96e-62 204
MS.gene56406.t1 AT4G16260 34.670 349 211 6 8 352 1 336 6.22e-62 203
MS.gene56406.t1 AT2G39640 35.942 345 208 7 10 350 5 340 1.27e-61 208
MS.gene56406.t1 AT5G20330 37.537 341 190 8 14 342 8 337 2.21e-61 202
MS.gene56406.t1 AT3G57260 36.982 338 195 10 13 348 15 336 1.05e-60 200
MS.gene56406.t1 AT1G33220 37.278 338 189 8 14 338 8 335 1.19e-60 199
MS.gene56406.t1 AT3G57260 36.982 338 195 10 13 348 31 352 1.89e-60 199
MS.gene56406.t1 AT1G77780 33.807 352 220 9 13 362 4 344 1.16e-59 198
MS.gene56406.t1 AT4G16260 35.350 314 195 4 39 352 2 307 1.53e-58 193
MS.gene56406.t1 AT5G24318 45.662 219 113 3 143 359 3 217 1.05e-57 191
MS.gene56406.t1 AT1G77790 35.174 344 210 8 10 348 6 341 5.00e-57 191
MS.gene56406.t1 AT3G57240 34.462 325 198 8 25 348 28 338 2.60e-52 178
MS.gene56406.t1 AT4G31140 30.899 356 229 11 4 352 3 348 3.52e-47 168
MS.gene56406.t1 AT3G24330 32.861 353 216 14 13 352 18 362 3.54e-46 166
MS.gene56406.t1 AT5G20560 32.907 313 194 8 33 340 35 336 2.07e-45 160
MS.gene56406.t1 AT5G64790 33.230 322 198 11 29 341 25 338 6.11e-45 162
MS.gene56406.t1 AT5G18220 31.818 330 212 8 29 352 27 349 3.65e-43 157
MS.gene56406.t1 AT1G64760 31.953 338 211 11 29 356 22 350 7.97e-42 154
MS.gene56406.t1 AT1G64760 31.953 338 211 11 29 356 22 350 7.97e-42 154
MS.gene56406.t1 AT2G19440 31.609 348 221 11 14 352 3 342 7.99e-42 153
MS.gene56406.t1 AT2G19440 31.609 348 221 11 14 352 3 342 7.99e-42 153
MS.gene56406.t1 AT4G17180 30.514 331 210 11 30 351 23 342 1.62e-41 152
MS.gene56406.t1 AT5G58480 31.804 327 208 9 29 347 23 342 2.10e-41 152
MS.gene56406.t1 AT5G58090 30.422 332 218 9 28 352 18 343 3.28e-41 152
MS.gene56406.t1 AT5G20870 31.680 363 221 14 5 351 6 357 1.05e-40 151
MS.gene56406.t1 AT3G55780 32.390 318 199 10 46 355 49 358 1.31e-40 148
MS.gene56406.t1 AT3G04010 32.087 321 203 10 29 341 30 343 2.29e-39 147

Find 67 sgRNAs with CRISPR-Local

Find 91 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CTGGAACAAGATATTGAATT+AGG 0.153002 4.4:-28639708 None:intergenic
ACATTTGCCATTGCTAAATT+AGG 0.171344 4.4:+28640066 MS.gene56406:CDS
TATGATTCCAATCTCATTCT+AGG 0.189809 4.4:-28640237 None:intergenic
TCATAATGTAAATTTGTATT+AGG 0.242481 4.4:-28640012 None:intergenic
GATAATTTCCACGTCTGAAT+TGG 0.275744 4.4:-28639536 None:intergenic
TTCGCACGTGTCGCGTCATT+TGG 0.301924 4.4:+28639388 MS.gene56406:CDS
TAGTCTAGAGATATTTGATT+AGG 0.302492 4.4:-28639955 None:intergenic
TACAATGCTCTAGTTCAATT+AGG 0.318143 4.4:+28639745 MS.gene56406:CDS
TGCTCTAGTTCAATTAGGTT+TGG 0.329748 4.4:+28639750 MS.gene56406:CDS
CTTGTTGTGGATCATTTAAT+TGG 0.341370 4.4:-28639585 None:intergenic
ATCTTTGTGTCTGGAAGATA+TGG 0.358675 4.4:-28639637 None:intergenic
GAGGTTAGGGTTTCTGAGAC+TGG 0.362945 4.4:+28640102 MS.gene56406:CDS
AGTCTAGAGATATTTGATTA+GGG 0.367834 4.4:-28639954 None:intergenic
GTAAGAGAAATTGAAGTAGA+AGG 0.368771 4.4:-28640387 None:intergenic
TTTGAATCCTAGAATGAGAT+TGG 0.371338 4.4:+28640230 MS.gene56406:CDS
AAAGGTGACTCTGATGAAAT+TGG 0.380257 4.4:+28640135 MS.gene56406:CDS
GGATCAACCATTCCTTGGTT+AGG 0.386797 4.4:-28639991 None:intergenic
TCATTCTTATAAGCAAAATA+AGG 0.388117 4.4:-28639931 None:intergenic
GCGTCATTTGGAATCAACTA+TGG 0.393043 4.4:+28639400 MS.gene56406:CDS
TCTCTTACCTCTTCTGCCAC+TGG 0.396125 4.4:+28640402 MS.gene56406:CDS
ACATCCAGTGGAATAAGCTT+TGG 0.403600 4.4:-28641091 None:intergenic
TTTAATGAAAATATGAAGAA+TGG 0.404108 4.4:+28640273 MS.gene56406:CDS
TGGAACAAGATATTGAATTA+GGG 0.410253 4.4:-28639707 None:intergenic
CTCTTACCTCTTCTGCCACT+GGG 0.413547 4.4:+28640403 MS.gene56406:CDS
TGGAAGATATGGTTTGATAT+TGG 0.415025 4.4:-28639626 None:intergenic
CCAACCTCAGAAAGGAACTA+TGG 0.427631 4.4:+28640297 MS.gene56406:CDS
CCATAGTTCCTTTCTGAGGT+TGG 0.438349 4.4:-28640297 None:intergenic
TAGATAGTCTTCTTAAACAA+AGG 0.440451 4.4:-28641144 None:intergenic
TATTAGGATCAACCATTCCT+TGG 0.446836 4.4:-28639996 None:intergenic
ATCCACAACAAGCACTTCAA+TGG 0.448948 4.4:+28639596 MS.gene56406:CDS
TGATAACATGCTTTATGCTA+TGG 0.465658 4.4:+28640032 MS.gene56406:CDS
AGGATATGCATTGACCCAAA+AGG 0.470969 4.4:-28639911 None:intergenic
TTGTAAGTCATACTTTCATC+AGG 0.472826 4.4:-28640330 None:intergenic
AAAGTATGACTTACAATGTT+GGG 0.473433 4.4:+28640337 MS.gene56406:CDS
TTTGAAGCAGGCATCACAAA+AGG 0.474431 4.4:+28641064 MS.gene56406:intron
ATTCCAGTGATCTTTGTGTC+TGG 0.474898 4.4:-28639646 None:intergenic
AAGATCACTGGAATTCAAGT+AGG 0.477367 4.4:+28639655 MS.gene56406:CDS
CTCTTCTGCCACTGGGACAA+AGG 0.479527 4.4:+28640410 MS.gene56406:CDS
GGCAACATTTACTCAAAGAA+TGG 0.488334 4.4:+28639213 MS.gene56406:CDS
GGATATGCATTGACCCAAAA+GGG 0.491865 4.4:-28639910 None:intergenic
TTGTAAATGTTGATCACTGC+TGG 0.497136 4.4:-28639727 None:intergenic
TTTATAACAAGAATTTATTG+AGG 0.499075 4.4:+28640181 MS.gene56406:CDS
TTAGGGTTTCTGAGACTGGT+TGG 0.504743 4.4:+28640106 MS.gene56406:CDS
TCAAGAACTTCTAATGAACT+AGG 0.514280 4.4:-28639796 None:intergenic
AATCTCATTCTAGGATTCAA+AGG 0.515011 4.4:-28640228 None:intergenic
AGTTTCAGAAGTGAAATCTC+TGG 0.515867 4.4:+28639844 MS.gene56406:CDS
GAGACTGGTTGGCCATCAAA+AGG 0.519791 4.4:+28640117 MS.gene56406:CDS
TCCACAACAAGCACTTCAAT+GGG 0.523957 4.4:+28639597 MS.gene56406:CDS
ATTTAATCCTAACCAAGGAA+TGG 0.526689 4.4:+28639984 MS.gene56406:CDS
CTTTGTCCCAGTGGCAGAAG+AGG 0.529705 4.4:-28640409 None:intergenic
AAAACCAAAGCTTATTCCAC+TGG 0.534591 4.4:+28641087 MS.gene56406:CDS
AGGAGACAAATGAAGAATGA+AGG 0.534907 4.4:+28640201 MS.gene56406:CDS
CTTCCAGACACAAAGATCAC+TGG 0.544255 4.4:+28639643 MS.gene56406:CDS
CTGAAACTACCAACTGAAGG+AGG 0.545386 4.4:-28639829 None:intergenic
ACCCATTGAAGTGCTTGTTG+TGG 0.545635 4.4:-28639598 None:intergenic
GCGAAAGCTTTAAGAATCTG+TGG 0.549065 4.4:-28639514 None:intergenic
GAATCTTACCCTCCTTCAGT+TGG 0.549919 4.4:+28639820 MS.gene56406:CDS
ATTAAAACCTAATTTAGCAA+TGG 0.564223 4.4:-28640073 None:intergenic
TAAACCATAGTTCCTTTCTG+AGG 0.569646 4.4:-28640301 None:intergenic
GAAAGTATGACTTACAATGT+TGG 0.572069 4.4:+28640336 MS.gene56406:CDS
AGATCACTGGAATTCAAGTA+GGG 0.581675 4.4:+28639656 MS.gene56406:CDS
AGCTTTCGCCAATTCAGACG+TGG 0.610463 4.4:+28639528 MS.gene56406:CDS
AGAATGGACCAACCTCAGAA+AGG 0.610841 4.4:+28640289 MS.gene56406:CDS
CTTCTGAAACTACCAACTGA+AGG 0.615063 4.4:-28639832 None:intergenic
GTATTATTTAATCCTAACCA+AGG 0.619362 4.4:+28639979 MS.gene56406:CDS
GCAAATACACAAACATCCAG+TGG 0.623842 4.4:-28641103 None:intergenic
TGAAACTACCAACTGAAGGA+GGG 0.625505 4.4:-28639828 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! AATTATTTAAAGTTATTGTA+TGG + chr4.4:28640715-28640734 MS.gene56406:intron 10.0%
!!! ATATATAGATTATGTTTTTT+TGG + chr4.4:28640629-28640648 MS.gene56406:intron 10.0%
!! AAAGTATATAATTTAGATCA+TGG + chr4.4:28640529-28640548 MS.gene56406:intron 15.0%
!! CAATAACTTTAAATAATTCA+AGG - chr4.4:28640713-28640732 None:intergenic 15.0%
!! TCATAATGTAAATTTGTATT+AGG - chr4.4:28640015-28640034 None:intergenic 15.0%
!! TTTAATGAAAATATGAAGAA+TGG + chr4.4:28640273-28640292 MS.gene56406:CDS 15.0%
!! TTTATAACAAGAATTTATTG+AGG + chr4.4:28640181-28640200 MS.gene56406:CDS 15.0%
!! ATTAAAACCTAATTTAGCAA+TGG - chr4.4:28640076-28640095 None:intergenic 20.0%
!! TCATTCTTATAAGCAAAATA+AGG - chr4.4:28639934-28639953 None:intergenic 20.0%
!!! ATTGCTAAATTAGGTTTTAA+TGG + chr4.4:28640075-28640094 MS.gene56406:CDS 20.0%
!!! CTAAATAACTTGTATATTGT+TGG - chr4.4:28640480-28640499 None:intergenic 20.0%
!!! TTGCTAAATTAGGTTTTAAT+GGG + chr4.4:28640076-28640095 MS.gene56406:CDS 20.0%
! AAAGTATGACTTACAATGTT+GGG + chr4.4:28640337-28640356 MS.gene56406:CDS 25.0%
! AGTCTAGAGATATTTGATTA+GGG - chr4.4:28639957-28639976 None:intergenic 25.0%
! ATAATTTAGATCATGGTTGT+TGG + chr4.4:28640536-28640555 MS.gene56406:intron 25.0%
! ATGAAAAAACAGAACAAACA+GGG - chr4.4:28639281-28639300 None:intergenic 25.0%
! GTATTATTTAATCCTAACCA+AGG + chr4.4:28639979-28639998 MS.gene56406:CDS 25.0%
! TAGTCTAGAGATATTTGATT+AGG - chr4.4:28639958-28639977 None:intergenic 25.0%
! TGGAACAAGATATTGAATTA+GGG - chr4.4:28639710-28639729 None:intergenic 25.0%
!! AAAAACAAAATCACCCTTTT+GGG + chr4.4:28639897-28639916 MS.gene56406:CDS 25.0%
!!! ATCAAATTTTACTTTGAAGC+AGG + chr4.4:28641052-28641071 MS.gene56406:intron 25.0%
!!! TCTTTCTTTTGTCTAAAACA+CGG - chr4.4:28640885-28640904 None:intergenic 25.0%
ACATTTGCCATTGCTAAATT+AGG + chr4.4:28640066-28640085 MS.gene56406:CDS 30.0%
ATTTAATCCTAACCAAGGAA+TGG + chr4.4:28639984-28640003 MS.gene56406:CDS 30.0%
CATGAAAAAACAGAACAAAC+AGG - chr4.4:28639282-28639301 None:intergenic 30.0%
CTGGAACAAGATATTGAATT+AGG - chr4.4:28639711-28639730 None:intergenic 30.0%
GATTATGCAATTACAGATAC+TGG + chr4.4:28639361-28639380 MS.gene56406:intron 30.0%
GTAAGAGAAATTGAAGTAGA+AGG - chr4.4:28640390-28640409 None:intergenic 30.0%
TATGATTCCAATCTCATTCT+AGG - chr4.4:28640240-28640259 None:intergenic 30.0%
TCAAGAACTTCTAATGAACT+AGG - chr4.4:28639799-28639818 None:intergenic 30.0%
TGATAACATGCTTTATGCTA+TGG + chr4.4:28640032-28640051 MS.gene56406:CDS 30.0%
TGGAAGATATGGTTTGATAT+TGG - chr4.4:28639629-28639648 None:intergenic 30.0%
TTGTAAGTCATACTTTCATC+AGG - chr4.4:28640333-28640352 None:intergenic 30.0%
TTTGAATCCTAGAATGAGAT+TGG + chr4.4:28640230-28640249 MS.gene56406:CDS 30.0%
! CAAAAACAAAATCACCCTTT+TGG + chr4.4:28639896-28639915 MS.gene56406:CDS 30.0%
! CTTGTTGTGGATCATTTAAT+TGG - chr4.4:28639588-28639607 None:intergenic 30.0%
! GAAAGTATGACTTACAATGT+TGG + chr4.4:28640336-28640355 MS.gene56406:CDS 30.0%
! TACAATGCTCTAGTTCAATT+AGG + chr4.4:28639745-28639764 MS.gene56406:CDS 30.0%
! TTTTAATGGGATAGAGGTTA+GGG + chr4.4:28640089-28640108 MS.gene56406:CDS 30.0%
!! AATCTCATTCTAGGATTCAA+AGG - chr4.4:28640231-28640250 None:intergenic 30.0%
!! ATTAGGTTTTAATGGGATAG+AGG + chr4.4:28640083-28640102 MS.gene56406:CDS 30.0%
AAAACCAAAGCTTATTCCAC+TGG + chr4.4:28641087-28641106 MS.gene56406:CDS 35.0%
AAAGGTGACTCTGATGAAAT+TGG + chr4.4:28640135-28640154 MS.gene56406:CDS 35.0%
AAGATCACTGGAATTCAAGT+AGG + chr4.4:28639655-28639674 MS.gene56406:CDS 35.0%
AGATCACTGGAATTCAAGTA+GGG + chr4.4:28639656-28639675 MS.gene56406:CDS 35.0%
AGGAGACAAATGAAGAATGA+AGG + chr4.4:28640201-28640220 MS.gene56406:CDS 35.0%
AGTTTCAGAAGTGAAATCTC+TGG + chr4.4:28639844-28639863 MS.gene56406:CDS 35.0%
ATCTTTGTGTCTGGAAGATA+TGG - chr4.4:28639640-28639659 None:intergenic 35.0%
GACATAGACAAAATCATAGC+TGG - chr4.4:28640745-28640764 None:intergenic 35.0%
GATAATTTCCACGTCTGAAT+TGG - chr4.4:28639539-28639558 None:intergenic 35.0%
GGCAACATTTACTCAAAGAA+TGG + chr4.4:28639213-28639232 MS.gene56406:CDS 35.0%
TAAACCATAGTTCCTTTCTG+AGG - chr4.4:28640304-28640323 None:intergenic 35.0%
TATTAGGATCAACCATTCCT+TGG - chr4.4:28639999-28640018 None:intergenic 35.0%
TTGTAAATGTTGATCACTGC+TGG - chr4.4:28639730-28639749 None:intergenic 35.0%
! TGCTCTAGTTCAATTAGGTT+TGG + chr4.4:28639750-28639769 MS.gene56406:CDS 35.0%
!! GTTTTAATGGGATAGAGGTT+AGG + chr4.4:28640088-28640107 MS.gene56406:CDS 35.0%
!!! AGAAGTCCTAGTACTTTTTC+TGG - chr4.4:28639451-28639470 None:intergenic 35.0%
!!! TTTTTTTACCTTTGTCCCAG+TGG - chr4.4:28640421-28640440 None:intergenic 35.0%
ACATCCAGTGGAATAAGCTT+TGG - chr4.4:28641094-28641113 None:intergenic 40.0%
ATCCACAACAAGCACTTCAA+TGG + chr4.4:28639596-28639615 MS.gene56406:CDS 40.0%
ATTCCAGTGATCTTTGTGTC+TGG - chr4.4:28639649-28639668 None:intergenic 40.0%
GCAAATACACAAACATCCAG+TGG - chr4.4:28641106-28641125 None:intergenic 40.0%
TCCACAACAAGCACTTCAAT+GGG + chr4.4:28639597-28639616 MS.gene56406:CDS 40.0%
TGAAACTACCAACTGAAGGA+GGG - chr4.4:28639831-28639850 None:intergenic 40.0%
! AGGATATGCATTGACCCAAA+AGG - chr4.4:28639914-28639933 None:intergenic 40.0%
! GCGTCATTTGGAATCAACTA+TGG + chr4.4:28639400-28639419 MS.gene56406:CDS 40.0%
! GGATATGCATTGACCCAAAA+GGG - chr4.4:28639913-28639932 None:intergenic 40.0%
! TCATCAGAGTCACCTTTTGA+TGG - chr4.4:28640132-28640151 None:intergenic 40.0%
! TTTGAAGCAGGCATCACAAA+AGG + chr4.4:28641064-28641083 MS.gene56406:intron 40.0%
!! CCACAACCAGAAAAAGTACT+AGG + chr4.4:28639442-28639461 MS.gene56406:CDS 40.0%
!! CTTCTGAAACTACCAACTGA+AGG - chr4.4:28639835-28639854 None:intergenic 40.0%
!! GCGAAAGCTTTAAGAATCTG+TGG - chr4.4:28639517-28639536 None:intergenic 40.0%
!!! CCTAGTACTTTTTCTGGTTG+TGG - chr4.4:28639445-28639464 None:intergenic 40.0%
!!! CTAGCATTTTTTCTCTCTGC+AGG + chr4.4:28639253-28639272 MS.gene56406:CDS 40.0%
AGAATGGACCAACCTCAGAA+AGG + chr4.4:28640289-28640308 MS.gene56406:CDS 45.0%
CCAACCTCAGAAAGGAACTA+TGG + chr4.4:28640297-28640316 MS.gene56406:CDS 45.0%
CTGAAACTACCAACTGAAGG+AGG - chr4.4:28639832-28639851 None:intergenic 45.0%
CTTCCAGACACAAAGATCAC+TGG + chr4.4:28639643-28639662 MS.gene56406:CDS 45.0%
GAATCTTACCCTCCTTCAGT+TGG + chr4.4:28639820-28639839 MS.gene56406:CDS 45.0%
GGATCAACCATTCCTTGGTT+AGG - chr4.4:28639994-28640013 None:intergenic 45.0%
! ACCCATTGAAGTGCTTGTTG+TGG - chr4.4:28639601-28639620 None:intergenic 45.0%
!! CCATAGTTCCTTTCTGAGGT+TGG - chr4.4:28640300-28640319 None:intergenic 45.0%
!! TTAGGGTTTCTGAGACTGGT+TGG + chr4.4:28640106-28640125 MS.gene56406:CDS 45.0%
AGCTTTCGCCAATTCAGACG+TGG + chr4.4:28639528-28639547 MS.gene56406:CDS 50.0%
CTCTTACCTCTTCTGCCACT+GGG + chr4.4:28640403-28640422 MS.gene56406:CDS 50.0%
TCTCTTACCTCTTCTGCCAC+TGG + chr4.4:28640402-28640421 MS.gene56406:CDS 50.0%
! GAGACTGGTTGGCCATCAAA+AGG + chr4.4:28640117-28640136 MS.gene56406:CDS 50.0%
!! GAGGTTAGGGTTTCTGAGAC+TGG + chr4.4:28640102-28640121 MS.gene56406:CDS 50.0%
CTCTTCTGCCACTGGGACAA+AGG + chr4.4:28640410-28640429 MS.gene56406:CDS 55.0%
CTTTGTCCCAGTGGCAGAAG+AGG - chr4.4:28640412-28640431 None:intergenic 55.0%
TTCGCACGTGTCGCGTCATT+TGG + chr4.4:28639388-28639407 MS.gene56406:CDS 55.0%


Chromosome Type Strat End Strand Name
chr4.4 gene 28639211 28641154 28639211 ID=MS.gene56406
chr4.4 mRNA 28639211 28641154 28639211 ID=MS.gene56406.t1;Parent=MS.gene56406
chr4.4 exon 28639211 28639274 28639211 ID=MS.gene56406.t1.exon1;Parent=MS.gene56406.t1
chr4.4 CDS 28639211 28639274 28639211 ID=cds.MS.gene56406.t1;Parent=MS.gene56406.t1
chr4.4 exon 28639377 28640431 28639377 ID=MS.gene56406.t1.exon2;Parent=MS.gene56406.t1
chr4.4 CDS 28639377 28640431 28639377 ID=cds.MS.gene56406.t1;Parent=MS.gene56406.t1
chr4.4 exon 28641074 28641154 28641074 ID=MS.gene56406.t1.exon3;Parent=MS.gene56406.t1
chr4.4 CDS 28641074 28641154 28641074 ID=cds.MS.gene56406.t1;Parent=MS.gene56406.t1
Gene Sequence

>MS.gene56406

ATGGCAACATTTACTCAAAGAATGGCAATACGTTATATTCTTCTAGCATTTTTTCTCTCTGCAGGTACCCTGTTTGTTCTGTTTTTTCATGCATGTTATCTTATCTTTATTGCATTCTAGTTTGTAACATTATTGCTAATAGATTTTACTGATTATGCAATTACAGATACTGGATTTTTCGCACGTGTCGCGTCATTTGGAATCAACTATGGACAAGTAGCTAACAATTTGCCACAACCAGAAAAAGTACTAGGACTTCTCAGCAGCTTAAAGATCACAAAAACAAGAATCTACGATACGAATCCACAGATTCTTAAAGCTTTCGCCAATTCAGACGTGGAAATTATCGTAACAATTGAAAATCAAATACTAAACCAATTAAATGATCCACAACAAGCACTTCAATGGGTAAATACCAATATCAAACCATATCTTCCAGACACAAAGATCACTGGAATTCAAGTAGGGAATGAAGTGTTCACAGAACTAGACACAACCCTAATTCAATATCTTGTTCCAGCAGTGATCAACATTTACAATGCTCTAGTTCAATTAGGTTTGGACTCAAACATTCACGTGTCAACACCTAGTTCATTAGAAGTTCTTGAAGAATCTTACCCTCCTTCAGTTGGTAGTTTCAGAAGTGAAATCTCTGGAATCATGCATGATTTTTTGAACTTTTTGTCAAAAACAAAATCACCCTTTTGGGTCAATGCATATCCTTATTTTGCTTATAAGAATGACCCTAATCAAATATCTCTAGACTATGTATTATTTAATCCTAACCAAGGAATGGTTGATCCTAATACAAATTTACATTATGATAACATGCTTTATGCTATGGTAGATGCTGTTACATTTGCCATTGCTAAATTAGGTTTTAATGGGATAGAGGTTAGGGTTTCTGAGACTGGTTGGCCATCAAAAGGTGACTCTGATGAAATTGGTGCATCATTGAAAAATGCTGAAATTTATAACAAGAATTTATTGAGGAGACAAATGAAGAATGAAGGAACACCTTTGAATCCTAGAATGAGATTGGAATCATATTTGTTTGCATTATTTAATGAAAATATGAAGAATGGACCAACCTCAGAAAGGAACTATGGTTTATTTGAGCCTGATGAAAGTATGACTTACAATGTTGGGTTGTCTTCTTTTGCAAAGTCATCAAATCCTTCTACTTCAATTTCTCTTACCTCTTCTGCCACTGGGACAAAGGTAAAAAAAAAAAGAGTAAAGACATTGTTTTTTTTTCTTTCTTTTCCAACAATATACAAGTTATTTAGCTTATAGTAAAATTTTGTGATAGAAAATGAAAGTATATAATTTAGATCATGGTTGTTGGTATCTTTGAAGTGCATGCAAATTGTGAGTATATATACTTTCGACATGCAGAAGAATTTAGATTCATTTTGATATATAGATTATGTTTTTTTGGAGTTGAACATTTGTGAAGCAAACAGTTTTTATTTGTTATTTTGATCTATAACTTAGAACCTTGAATTATTTAAAGTTATTGTATGGTACACCAGCTATGATTTTGTCTATGTCACATGCTTTTTTTAAATATTTTTATTGATCGTGCATCTTATGACAATTTATAATATTTGAATCATAATTTAAAAAAAATATGTCTTTTGATACACGATTTGCATTTTGTTTCAATGACCGTGTTTTAGACAAAAGAAAGAAATGTATATCAATTTTAAAAGAAAGATGTCTAGTGACATATATTAGTCAAATATTTACTTGAAGTATACTATGACTTTTTTGCTATTTTTAGCACTTTATTATATATATTAATTTGATCAACATCAATCTGATTATCTTCTTTTCAAATCAAATTTTACTTTGAAGCAGGCATCACAAAAGGAAAACCAAAGCTTATTCCACTGGATGTTTGTGTATTTGCTGATTTCATCACTTTGTGCCTTTGTTTAA
Protein sequence

>MS.gene56406.t1

MATFTQRMAIRYILLAFFLSADTGFFARVASFGINYGQVANNLPQPEKVLGLLSSLKITKTRIYDTNPQILKAFANSDVEIIVTIENQILNQLNDPQQALQWVNTNIKPYLPDTKITGIQVGNEVFTELDTTLIQYLVPAVINIYNALVQLGLDSNIHVSTPSSLEVLEESYPPSVGSFRSEISGIMHDFLNFLSKTKSPFWVNAYPYFAYKNDPNQISLDYVLFNPNQGMVDPNTNLHYDNMLYAMVDAVTFAIAKLGFNGIEVRVSETGWPSKGDSDEIGASLKNAEIYNKNLLRRQMKNEGTPLNPRMRLESYLFALFNENMKNGPTSERNYGLFEPDESMTYNVGLSSFAKSSNPSTSISLTSSATGTKASQKENQSLFHWMFVYLLISSLCAFV