AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene059510

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene059510.t1 MTR_4g084480 96.742 399 13 0 1 399 1 399 0.0 789
MS.gene059510.t1 MTR_4g084480 94.286 385 22 0 1 385 1 385 0.0 739
MS.gene059510.t1 MTR_4g084480 96.721 366 12 0 8 373 1 366 0.0 721
MS.gene059510.t1 MTR_2g019345 70.992 393 99 3 12 395 9 395 0.0 551
MS.gene059510.t1 MTR_1g077990 56.231 329 143 1 23 351 22 349 4.63e-136 395
MS.gene059510.t1 MTR_4g052970 52.632 323 153 0 31 353 28 350 8.91e-126 368
MS.gene059510.t1 MTR_2g086530 49.563 343 170 2 9 350 5 345 8.58e-123 361
MS.gene059510.t1 MTR_2g086530 52.038 319 151 1 32 350 8 324 7.16e-122 357
MS.gene059510.t1 MTR_4g010200 48.368 337 173 1 30 365 31 367 4.97e-117 346
MS.gene059510.t1 MTR_4g114850 45.846 325 175 1 30 353 24 348 1.21e-102 309
MS.gene059510.t1 MTR_4g114850 45.846 325 175 1 30 353 24 348 1.28e-102 308
MS.gene059510.t1 MTR_4g114850 45.846 325 175 1 30 353 24 348 1.44e-102 309
MS.gene059510.t1 MTR_5g085580 49.231 325 162 3 29 351 22 345 2.10e-99 304
MS.gene059510.t1 MTR_3g116510 43.407 364 199 3 1 362 1 359 7.83e-99 302
MS.gene059510.t1 MTR_4g069940 44.444 351 193 2 14 362 10 360 7.10e-97 297
MS.gene059510.t1 MTR_1g022160 46.584 322 170 2 32 351 24 345 7.22e-97 295
MS.gene059510.t1 MTR_1g022160 46.584 322 170 2 32 351 24 345 2.30e-96 296
MS.gene059510.t1 MTR_1g022160 46.584 322 170 2 32 351 24 345 3.33e-96 296
MS.gene059510.t1 MTR_1g022160 46.584 322 170 2 32 351 24 345 4.32e-96 296
MS.gene059510.t1 MTR_1g056640 45.706 326 174 3 28 351 34 358 3.86e-95 293
MS.gene059510.t1 MTR_3g080410 45.294 340 178 4 30 362 24 362 6.97e-95 288
MS.gene059510.t1 MTR_1g007810 45.679 324 170 3 26 347 10 329 1.45e-92 283
MS.gene059510.t1 MTR_6g088885 42.265 362 198 4 33 385 28 387 1.88e-88 277
MS.gene059510.t1 MTR_7g026340 44.753 324 171 5 29 350 20 337 2.13e-86 270
MS.gene059510.t1 MTR_7g053500 42.462 325 181 5 33 352 27 350 2.65e-86 270
MS.gene059510.t1 MTR_8g012400 38.655 357 210 6 1 352 1 353 3.43e-82 259
MS.gene059510.t1 MTR_4g088155 39.394 330 192 5 30 352 21 349 1.13e-78 250
MS.gene059510.t1 MTR_4g083500 41.667 348 194 7 7 350 19 361 5.36e-77 248
MS.gene059510.t1 MTR_3g083580 37.576 330 200 4 14 338 7 335 2.74e-76 245
MS.gene059510.t1 MTR_4g132280 39.144 327 194 3 32 354 45 370 4.36e-75 243
MS.gene059510.t1 MTR_1g031900 36.649 382 235 5 8 385 1 379 1.52e-74 241
MS.gene059510.t1 MTR_1g031900 36.649 382 235 5 8 385 1 379 3.81e-74 239
MS.gene059510.t1 MTR_8g102340 38.719 359 179 5 33 385 29 352 6.08e-74 238
MS.gene059510.t1 MTR_8g085720 37.383 321 196 3 30 346 24 343 7.95e-74 238
MS.gene059510.t1 MTR_4g076490 40.123 324 182 6 30 353 95 406 7.94e-73 234
MS.gene059510.t1 MTR_5g078200 36.623 385 230 6 8 385 1 378 2.81e-71 235
MS.gene059510.t1 MTR_3g065460 36.826 334 206 3 10 339 2 334 2.61e-69 226
MS.gene059510.t1 MTR_5g078200 37.464 347 211 4 43 385 1 345 1.15e-67 225
MS.gene059510.t1 MTR_4g076440 37.537 341 198 10 8 348 1 326 1.08e-66 215
MS.gene059510.t1 MTR_4g076470 38.053 339 195 9 8 346 1 324 1.20e-66 215
MS.gene059510.t1 MTR_7g053500 43.750 256 139 4 101 352 19 273 2.42e-66 216
MS.gene059510.t1 MTR_4g076430 37.719 342 196 10 8 348 1 326 3.53e-66 214
MS.gene059510.t1 MTR_1g052430 39.308 318 183 6 33 348 33 342 5.40e-66 216
MS.gene059510.t1 MTR_4g076255 39.009 323 184 6 26 348 19 328 1.57e-65 212
MS.gene059510.t1 MTR_4g076570 38.415 328 192 7 24 349 14 333 1.13e-63 208
MS.gene059510.t1 MTR_2g034480 38.769 325 189 7 30 352 25 341 2.11e-63 207
MS.gene059510.t1 MTR_7g081350 33.431 341 214 7 7 346 5 333 6.26e-63 206
MS.gene059510.t1 MTR_3g116510 44.444 225 125 0 138 362 1 225 1.39e-62 204
MS.gene059510.t1 MTR_4g076500 38.264 311 182 5 31 340 77 378 6.37e-61 202
MS.gene059510.t1 MTR_8g012400 39.927 273 158 5 85 352 2 273 1.24e-60 201
MS.gene059510.t1 MTR_2g034440 36.825 315 187 8 30 341 20 325 1.44e-58 194
MS.gene059510.t1 MTR_2g034470 35.241 332 203 8 30 358 28 350 2.51e-58 194
MS.gene059510.t1 MTR_2g034470 34.985 323 198 8 39 358 2 315 3.74e-55 185
MS.gene059510.t1 MTR_7g081370 31.378 341 209 7 7 346 5 321 4.41e-55 185
MS.gene059510.t1 MTR_1g010120 33.989 356 214 13 6 352 4 347 1.90e-52 182
MS.gene059510.t1 MTR_4g112025 32.231 363 222 14 2 353 6 355 1.88e-48 172
MS.gene059510.t1 MTR_4g124440 31.180 356 226 13 6 352 4 349 2.15e-48 171
MS.gene059510.t1 MTR_2g027560 32.121 330 211 9 30 351 26 350 8.84e-48 170
MS.gene059510.t1 MTR_6g032820 32.653 343 206 11 12 341 9 339 1.23e-47 168
MS.gene059510.t1 MTR_7g085390 33.134 335 204 11 29 352 23 348 6.42e-46 164
MS.gene059510.t1 MTR_8g092070 29.577 355 235 11 13 359 15 362 1.57e-44 162
MS.gene059510.t1 MTR_1g053320 31.143 350 225 11 11 350 9 352 3.69e-44 160
MS.gene059510.t1 MTR_3g108600 30.226 354 214 11 5 352 7 333 8.21e-43 156
MS.gene059510.t1 MTR_5g081720 34.967 306 184 10 48 347 53 349 4.30e-42 152
MS.gene059510.t1 MTR_3g031770 32.335 334 209 10 31 356 26 350 6.58e-41 151
MS.gene059510.t1 MTR_3g095050 29.477 363 238 14 9 362 10 363 2.05e-38 144
MS.gene059510.t1 MTR_4g134280 35.565 239 142 9 119 351 3 235 2.61e-32 125
MS.gene059510.t1 MTR_4g036775 34.694 147 88 4 248 391 80 221 1.23e-19 87.4
MS.gene059510.t1 MTR_4g036680 34.694 147 88 4 248 391 80 221 2.78e-19 86.3
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene059510.t1 AT4G18340 61.340 388 144 1 3 390 5 386 7.65e-177 498
MS.gene059510.t1 AT4G18340 62.803 371 138 0 3 373 5 375 6.28e-176 496
MS.gene059510.t1 AT1G30080 61.399 386 146 1 9 391 13 398 1.92e-171 485
MS.gene059510.t1 AT1G30080 64.306 353 122 2 1 349 1 353 1.34e-168 477
MS.gene059510.t1 AT2G27500 49.304 359 170 3 33 390 30 377 1.82e-123 362
MS.gene059510.t1 AT2G27500 50.877 342 161 2 33 373 30 365 1.35e-121 358
MS.gene059510.t1 AT1G32860 50.155 323 159 1 31 351 28 350 1.95e-120 356
MS.gene059510.t1 AT5G42100 51.692 325 154 2 29 350 24 348 7.44e-116 344
MS.gene059510.t1 AT5G42100 51.692 325 154 2 29 350 24 348 9.75e-116 344
MS.gene059510.t1 AT3G15800 48.036 331 170 2 29 357 40 370 3.80e-114 339
MS.gene059510.t1 AT2G26600 43.860 342 190 2 29 369 31 371 7.50e-106 317
MS.gene059510.t1 AT4G26830 48.286 350 172 5 12 359 8 350 4.88e-105 318
MS.gene059510.t1 AT4G26830 48.936 329 162 4 33 359 30 354 2.53e-103 313
MS.gene059510.t1 AT5G55180 48.598 321 162 3 32 350 27 346 7.50e-101 307
MS.gene059510.t1 AT5G55180 48.598 321 162 3 32 350 27 346 8.57e-101 307
MS.gene059510.t1 AT2G27500 50.512 293 138 2 82 373 1 287 3.14e-100 300
MS.gene059510.t1 AT5G24318 45.399 326 172 3 27 350 25 346 2.37e-97 295
MS.gene059510.t1 AT5G24318 45.238 336 177 4 27 359 25 356 1.52e-96 296
MS.gene059510.t1 AT5G24318 45.238 336 177 4 27 359 25 356 3.51e-96 295
MS.gene059510.t1 AT2G05790 45.963 322 171 3 29 348 22 342 4.48e-96 295
MS.gene059510.t1 AT4G34480 44.798 346 185 2 13 357 12 352 1.80e-94 287
MS.gene059510.t1 AT5G42720 44.058 345 191 2 9 351 3 347 1.12e-93 288
MS.gene059510.t1 AT4G34480 43.236 377 204 4 13 385 12 382 3.96e-93 289
MS.gene059510.t1 AT2G16230 40.833 360 207 2 33 386 26 385 3.03e-91 284
MS.gene059510.t1 AT5G56590 43.925 321 178 2 33 351 27 347 7.84e-91 283
MS.gene059510.t1 AT2G26600 45.221 272 148 1 98 369 7 277 9.18e-85 260
MS.gene059510.t1 AT3G07320 40.923 325 186 5 33 352 26 349 1.08e-84 266
MS.gene059510.t1 AT3G13560 40.683 322 186 3 29 346 23 343 3.21e-80 255
MS.gene059510.t1 AT3G13560 40.683 322 186 3 29 346 23 343 3.21e-80 255
MS.gene059510.t1 AT3G13560 40.683 322 186 3 29 346 23 343 3.21e-80 255
MS.gene059510.t1 AT3G13560 40.683 322 186 3 29 346 23 343 3.21e-80 255
MS.gene059510.t1 AT3G13560 40.683 322 186 3 29 346 23 343 3.21e-80 255
MS.gene059510.t1 AT4G14080 38.720 328 196 4 29 352 39 365 2.02e-77 247
MS.gene059510.t1 AT3G23770 38.507 335 198 5 25 352 30 363 3.48e-77 247
MS.gene059510.t1 AT1G11820 39.329 328 194 3 33 356 45 371 6.76e-77 243
MS.gene059510.t1 AT1G11820 39.628 323 190 3 33 351 45 366 7.58e-76 243
MS.gene059510.t1 AT4G29360 42.236 322 184 2 26 346 21 341 8.17e-76 245
MS.gene059510.t1 AT1G11820 39.937 318 186 3 33 346 45 361 9.83e-76 244
MS.gene059510.t1 AT4G29360 42.236 322 184 2 26 346 31 351 1.15e-75 244
MS.gene059510.t1 AT2G01630 37.989 358 216 4 32 385 23 378 7.35e-75 238
MS.gene059510.t1 AT2G01630 37.989 358 216 4 32 385 23 378 1.70e-74 239
MS.gene059510.t1 AT2G01630 37.989 358 216 4 32 385 23 378 1.25e-73 238
MS.gene059510.t1 AT3G55430 37.228 368 213 10 29 386 26 385 1.40e-73 236
MS.gene059510.t1 AT3G46570 41.515 330 182 8 26 350 21 344 1.03e-69 224
MS.gene059510.t1 AT1G66250 37.151 358 218 5 32 385 32 386 9.47e-69 226
MS.gene059510.t1 AT3G57270 38.871 319 183 8 33 348 28 337 1.03e-65 213
MS.gene059510.t1 AT5G20390 37.101 345 205 8 1 342 1 336 9.68e-65 210
MS.gene059510.t1 AT4G34480 44.444 270 146 2 119 385 3 271 1.11e-63 209
MS.gene059510.t1 AT4G16260 34.870 347 213 6 8 352 1 336 3.52e-63 206
MS.gene059510.t1 AT5G20340 38.924 316 178 7 31 342 42 346 7.03e-63 206
MS.gene059510.t1 AT3G61810 36.061 330 189 6 32 351 55 372 9.80e-62 204
MS.gene059510.t1 AT1G77780 34.483 348 215 9 17 362 8 344 1.78e-61 202
MS.gene059510.t1 AT2G39640 36.070 341 205 7 14 350 9 340 2.38e-61 207
MS.gene059510.t1 AT3G57260 36.686 338 196 10 13 348 15 336 5.57e-60 198
MS.gene059510.t1 AT3G57260 36.686 338 196 10 13 348 31 352 8.77e-60 198
MS.gene059510.t1 AT5G20330 38.291 316 180 7 31 342 33 337 2.03e-59 197
MS.gene059510.t1 AT4G16260 35.350 314 195 4 39 352 2 307 5.32e-59 194
MS.gene059510.t1 AT1G33220 37.870 338 187 9 14 338 8 335 6.94e-59 195
MS.gene059510.t1 AT5G24318 45.662 219 113 3 143 359 3 217 3.77e-58 192
MS.gene059510.t1 AT1G77790 34.493 345 213 8 7 348 7 341 1.12e-57 192
MS.gene059510.t1 AT3G57240 34.579 321 195 8 29 348 32 338 1.72e-52 178
MS.gene059510.t1 AT4G31140 31.180 356 228 11 4 352 3 348 8.19e-48 170
MS.gene059510.t1 AT3G24330 32.222 360 222 14 4 352 14 362 1.26e-47 169
MS.gene059510.t1 AT5G20560 31.642 335 211 9 11 340 15 336 3.77e-46 162
MS.gene059510.t1 AT5G64790 31.765 340 215 11 29 359 25 356 3.58e-45 162
MS.gene059510.t1 AT5G18220 32.229 332 212 8 27 352 25 349 1.36e-44 161
MS.gene059510.t1 AT1G64760 32.249 338 210 11 29 356 22 350 2.14e-42 155
MS.gene059510.t1 AT1G64760 32.249 338 210 11 29 356 22 350 2.14e-42 155
MS.gene059510.t1 AT2G19440 31.897 348 220 11 14 352 3 342 3.29e-42 154
MS.gene059510.t1 AT2G19440 31.897 348 220 11 14 352 3 342 3.29e-42 154
MS.gene059510.t1 AT4G17180 30.514 331 210 11 30 351 23 342 7.38e-42 153
MS.gene059510.t1 AT5G20870 31.956 363 220 14 5 351 6 357 9.14e-42 154
MS.gene059510.t1 AT5G58090 30.422 332 218 9 28 352 18 343 1.02e-41 153
MS.gene059510.t1 AT5G58480 31.498 327 209 9 29 347 23 342 1.30e-41 153
MS.gene059510.t1 AT3G04010 31.965 341 213 11 9 341 14 343 5.10e-41 151
MS.gene059510.t1 AT3G55780 32.075 318 200 10 46 355 49 358 8.91e-41 149

Find 66 sgRNAs with CRISPR-Local

Find 96 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CTGGAACAAGATATTGAATT+AGG 0.153002 4.4:+27497103 None:intergenic
ACATTTGCCATTGCTAAATT+AGG 0.171344 4.4:-27496745 MS.gene059510:CDS
TATGATTCCAATCTCATTCT+AGG 0.189809 4.4:+27496574 None:intergenic
TCATAATGTAAATTTGTATT+AGG 0.242481 4.4:+27496799 None:intergenic
TTCGGACGTGTTGCGTCATT+TGG 0.288335 4.4:-27497423 MS.gene059510:CDS
TAGTCTAGAGATATTTGATT+AGG 0.302492 4.4:+27496856 None:intergenic
TACAATGCTCTAGTTCAATT+AGG 0.318143 4.4:-27497066 MS.gene059510:CDS
GCGTCATTTGGAATAAACTA+TGG 0.327515 4.4:-27497411 MS.gene059510:CDS
TGCTCTAGTTCAATTAGGTT+TGG 0.329748 4.4:-27497061 MS.gene059510:CDS
CTTGTTGTGGATCATTTAAT+TGG 0.341370 4.4:+27497226 None:intergenic
ATCTTTGTGTCTGGAAGATA+TGG 0.358675 4.4:+27497174 None:intergenic
GAGGTTAGGGTTTCTGAGAC+TGG 0.362945 4.4:-27496709 MS.gene059510:CDS
AGTCTAGAGATATTTGATTA+GGG 0.367834 4.4:+27496857 None:intergenic
GTAAGAGAAATTGAAGTAGA+AGG 0.368771 4.4:+27496424 None:intergenic
GATGATCTCCACGTCTGAAT+TGG 0.369675 4.4:+27497275 None:intergenic
TTTGAATCCTAGAATGAGAT+TGG 0.371338 4.4:-27496581 MS.gene059510:CDS
AAAGGTGACTCTGATGAAAT+TGG 0.380257 4.4:-27496676 MS.gene059510:CDS
GGATCAACCATTCCTTGGTT+AGG 0.386797 4.4:+27496820 None:intergenic
TCATTCTTATAAGCAAAATA+AGG 0.388117 4.4:+27496880 None:intergenic
ACATCCAGTGGAATAAGCTT+TGG 0.403600 4.4:+27495719 None:intergenic
TTTAATGAAAATATGAAGAA+TGG 0.404108 4.4:-27496538 MS.gene059510:CDS
TGGAACAAGATATTGAATTA+GGG 0.410253 4.4:+27497104 None:intergenic
TGGAAGATATGGTTTGATAT+TGG 0.415025 4.4:+27497185 None:intergenic
CCAACCTCAGAAAGGAACTA+TGG 0.427631 4.4:-27496514 MS.gene059510:CDS
TAGATAGTCTTCTTAAACAA+AGG 0.440451 4.4:+27495666 None:intergenic
CCATAGTTCCTTTCTGAGGT+TGG 0.446684 4.4:+27496514 None:intergenic
TATTAGGATCAACCATTCCT+TGG 0.446836 4.4:+27496815 None:intergenic
ATCCACAACAAGCACTTCAA+TGG 0.448948 4.4:-27497215 MS.gene059510:CDS
TGATAACATGCTTTATGCTA+TGG 0.465658 4.4:-27496779 MS.gene059510:CDS
AGGATATGCATTGACCCAAA+AGG 0.470969 4.4:+27496900 None:intergenic
TTGTAAGTCATACTTTCATC+AGG 0.472826 4.4:+27496481 None:intergenic
AAAGTATGACTTACAATGTT+GGG 0.473433 4.4:-27496474 MS.gene059510:CDS
TTTGAAGCAGGCATCACAAA+AGG 0.474431 4.4:-27495746 MS.gene059510:intron
ATTCCAGTGATCTTTGTGTC+TGG 0.474898 4.4:+27497165 None:intergenic
AAGATCACTGGAATTCAAGT+AGG 0.477367 4.4:-27497156 MS.gene059510:CDS
GGCAACATTTACTCAAAGAA+TGG 0.488334 4.4:-27497616 MS.gene059510:CDS
GGATATGCATTGACCCAAAA+GGG 0.491865 4.4:+27496901 None:intergenic
TTGTAAATGTTGATCACTGC+TGG 0.497136 4.4:+27497084 None:intergenic
TTTATAACAAGAATTTATTG+AGG 0.499075 4.4:-27496630 MS.gene059510:CDS
TTAGGGTTTCTGAGACTGGT+TGG 0.504743 4.4:-27496705 MS.gene059510:CDS
CTCTTACCTCTTCTGCCACT+AGG 0.508681 4.4:-27496408 MS.gene059510:CDS
TCAAGAACTTCTAATGAACT+AGG 0.514280 4.4:+27497015 None:intergenic
AATCTCATTCTAGGATTCAA+AGG 0.515011 4.4:+27496583 None:intergenic
AGTTTCAGAAGTGAAATCTC+TGG 0.515867 4.4:-27496967 MS.gene059510:CDS
GAATCTTACCCTCCTTCAGC+TGG 0.518663 4.4:-27496991 MS.gene059510:CDS
GAGACTGGTTGGCCATCAAA+AGG 0.519791 4.4:-27496694 MS.gene059510:CDS
TCCACAACAAGCACTTCAAT+GGG 0.523957 4.4:-27497214 MS.gene059510:CDS
ATTTAATCCTAACCAAGGAA+TGG 0.526689 4.4:-27496827 MS.gene059510:CDS
AAAACCAAAGCTTATTCCAC+TGG 0.534591 4.4:-27495723 MS.gene059510:CDS
AGGAGACAAATGAAGAATGA+AGG 0.534907 4.4:-27496610 MS.gene059510:CDS
CTTTGTCCTAGTGGCAGAAG+AGG 0.537253 4.4:+27496402 None:intergenic
CTTCCAGACACAAAGATCAC+TGG 0.544255 4.4:-27497168 MS.gene059510:CDS
CTCTTCTGCCACTAGGACAA+AGG 0.544384 4.4:-27496401 MS.gene059510:intron
ACCCATTGAAGTGCTTGTTG+TGG 0.545635 4.4:+27497213 None:intergenic
GCGAAAGCTTTAAGAATCTG+TGG 0.549065 4.4:+27497297 None:intergenic
ATTAAAACCTAATTTAGCAA+TGG 0.564223 4.4:+27496738 None:intergenic
CAAACCATAGTTCCTTTCTG+AGG 0.570996 4.4:+27496510 None:intergenic
GAAAGTATGACTTACAATGT+TGG 0.572069 4.4:-27496475 MS.gene059510:CDS
AGATCACTGGAATTCAAGTA+GGG 0.581675 4.4:-27497155 MS.gene059510:CDS
CTGAAACTACCAGCTGAAGG+AGG 0.582451 4.4:+27496982 None:intergenic
AGAATGGACCAACCTCAGAA+AGG 0.610841 4.4:-27496522 MS.gene059510:CDS
AGCTTTCGCCAATTCAGACG+TGG 0.617283 4.4:-27497283 MS.gene059510:CDS
GTATTATTTAATCCTAACCA+AGG 0.619362 4.4:-27496832 MS.gene059510:CDS
GCAAATACACAAACATCCAG+TGG 0.623842 4.4:+27495707 None:intergenic
TGAAACTACCAGCTGAAGGA+GGG 0.628259 4.4:+27496983 None:intergenic
CTTCTGAAACTACCAGCTGA+AGG 0.634495 4.4:+27496979 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! AATAACTTTAAAATAATTCA+AGG + chr4.4:27497197-27497216 None:intergenic 10.0%
!!! ATATATAGATTATGTTTTTT+TGG - chr4.4:27497113-27497132 MS.gene059510:CDS 10.0%
!!! ATTATTTTAAAGTTATTGTA+TGG - chr4.4:27497200-27497219 MS.gene059510:CDS 10.0%
!! AAAGTATATAATTTAGATCA+TGG - chr4.4:27497013-27497032 MS.gene059510:CDS 15.0%
!! TCATAATGTAAATTTGTATT+AGG + chr4.4:27496500-27496519 None:intergenic 15.0%
!! TTTAATGAAAATATGAAGAA+TGG - chr4.4:27496758-27496777 MS.gene059510:CDS 15.0%
!! TTTATAACAAGAATTTATTG+AGG - chr4.4:27496666-27496685 MS.gene059510:CDS 15.0%
!! ATTAAAACCTAATTTAGCAA+TGG + chr4.4:27496561-27496580 None:intergenic 20.0%
!! TCATTCTTATAAGCAAAATA+AGG + chr4.4:27496419-27496438 None:intergenic 20.0%
!!! ATTACAGATACAGAAATTTT+CGG - chr4.4:27495855-27495874 MS.gene059510:intron 20.0%
!!! ATTGCTAAATTAGGTTTTAA+TGG - chr4.4:27496560-27496579 MS.gene059510:CDS 20.0%
!!! CTAAATAACTTGTATATTGT+TGG + chr4.4:27496964-27496983 None:intergenic 20.0%
!!! TTGCTAAATTAGGTTTTAAT+GGG - chr4.4:27496561-27496580 MS.gene059510:CDS 20.0%
! AAAGTATGACTTACAATGTT+GGG - chr4.4:27496822-27496841 MS.gene059510:CDS 25.0%
! AGTCTAGAGATATTTGATTA+GGG + chr4.4:27496442-27496461 None:intergenic 25.0%
! ATAATTTAGATCATGGTTGT+TGG - chr4.4:27497020-27497039 MS.gene059510:CDS 25.0%
! GTATTATTTAATCCTAACCA+AGG - chr4.4:27496464-27496483 MS.gene059510:CDS 25.0%
! TAGTCTAGAGATATTTGATT+AGG + chr4.4:27496443-27496462 None:intergenic 25.0%
! TGCATGATGAAAAGAATAAT+AGG + chr4.4:27495770-27495789 None:intergenic 25.0%
! TGGAACAAGATATTGAATTA+GGG + chr4.4:27496195-27496214 None:intergenic 25.0%
!! AAAAACAAAATCACCCTTTT+GGG - chr4.4:27496382-27496401 MS.gene059510:intron 25.0%
!!! ATCAAATTTTACTTTGAAGC+AGG - chr4.4:27497538-27497557 MS.gene059510:intron 25.0%
!!! TCTTGTTTTTGTGATCTTTA+AGG + chr4.4:27495964-27495983 None:intergenic 25.0%
!!! TCTTTCTTTTGTCTAAAACA+CGG + chr4.4:27497370-27497389 None:intergenic 25.0%
ACATTTGCCATTGCTAAATT+AGG - chr4.4:27496551-27496570 MS.gene059510:CDS 30.0%
ATTTAATCCTAACCAAGGAA+TGG - chr4.4:27496469-27496488 MS.gene059510:CDS 30.0%
CTGGAACAAGATATTGAATT+AGG + chr4.4:27496196-27496215 None:intergenic 30.0%
GTAAGAGAAATTGAAGTAGA+AGG + chr4.4:27496875-27496894 None:intergenic 30.0%
GTGAAAAAACAGAACAAACA+GGG + chr4.4:27495748-27495767 None:intergenic 30.0%
TATGATTCCAATCTCATTCT+AGG + chr4.4:27496725-27496744 None:intergenic 30.0%
TCAAGAACTTCTAATGAACT+AGG + chr4.4:27496284-27496303 None:intergenic 30.0%
TGATAACATGCTTTATGCTA+TGG - chr4.4:27496517-27496536 MS.gene059510:CDS 30.0%
TGGAAGATATGGTTTGATAT+TGG + chr4.4:27496114-27496133 None:intergenic 30.0%
TTGTAAGTCATACTTTCATC+AGG + chr4.4:27496818-27496837 None:intergenic 30.0%
TTTGAATCCTAGAATGAGAT+TGG - chr4.4:27496715-27496734 MS.gene059510:CDS 30.0%
! CAAAAACAAAATCACCCTTT+TGG - chr4.4:27496381-27496400 MS.gene059510:intron 30.0%
! CTTGTTGTGGATCATTTAAT+TGG + chr4.4:27496073-27496092 None:intergenic 30.0%
! GAAAGTATGACTTACAATGT+TGG - chr4.4:27496821-27496840 MS.gene059510:CDS 30.0%
! TACAATGCTCTAGTTCAATT+AGG - chr4.4:27496230-27496249 MS.gene059510:intron 30.0%
! TTGATACACGATTTGCATTT+TGG - chr4.4:27497336-27497355 MS.gene059510:CDS 30.0%
! TTTTAATGGGATAGAGGTTA+GGG - chr4.4:27496574-27496593 MS.gene059510:CDS 30.0%
!! AATCTCATTCTAGGATTCAA+AGG + chr4.4:27496716-27496735 None:intergenic 30.0%
!! ATTAGGTTTTAATGGGATAG+AGG - chr4.4:27496568-27496587 MS.gene059510:CDS 30.0%
!!! TTTTTTTACCTTTGTCCTAG+TGG + chr4.4:27496906-27496925 None:intergenic 30.0%
AAAACCAAAGCTTATTCCAC+TGG - chr4.4:27497573-27497592 MS.gene059510:intron 35.0%
AAAGGTGACTCTGATGAAAT+TGG - chr4.4:27496620-27496639 MS.gene059510:CDS 35.0%
AAGATCACTGGAATTCAAGT+AGG - chr4.4:27496140-27496159 MS.gene059510:intron 35.0%
AGATCACTGGAATTCAAGTA+GGG - chr4.4:27496141-27496160 MS.gene059510:intron 35.0%
AGGAGACAAATGAAGAATGA+AGG - chr4.4:27496686-27496705 MS.gene059510:CDS 35.0%
AGTTTCAGAAGTGAAATCTC+TGG - chr4.4:27496329-27496348 MS.gene059510:intron 35.0%
ATCTTTGTGTCTGGAAGATA+TGG + chr4.4:27496125-27496144 None:intergenic 35.0%
GACATAGACAAAATCATAGC+TGG + chr4.4:27497230-27497249 None:intergenic 35.0%
GGCAACATTTACTCAAAGAA+TGG - chr4.4:27495680-27495699 MS.gene059510:CDS 35.0%
GGTGAAAAAACAGAACAAAC+AGG + chr4.4:27495749-27495768 None:intergenic 35.0%
TATTAGGATCAACCATTCCT+TGG + chr4.4:27496484-27496503 None:intergenic 35.0%
TTGAAGTGCATGCAAATTGT+GGG - chr4.4:27497048-27497067 MS.gene059510:CDS 35.0%
TTGTAAATGTTGATCACTGC+TGG + chr4.4:27496215-27496234 None:intergenic 35.0%
! GCGTCATTTGGAATAAACTA+TGG - chr4.4:27495885-27495904 MS.gene059510:intron 35.0%
! TGCTCTAGTTCAATTAGGTT+TGG - chr4.4:27496235-27496254 MS.gene059510:intron 35.0%
! TTTGAAGTGCATGCAAATTG+TGG - chr4.4:27497047-27497066 MS.gene059510:CDS 35.0%
!! CACAACCAGAAAAAGTACTA+GGG - chr4.4:27495928-27495947 MS.gene059510:intron 35.0%
!! GTTTTAATGGGATAGAGGTT+AGG - chr4.4:27496573-27496592 MS.gene059510:CDS 35.0%
ACATCCAGTGGAATAAGCTT+TGG + chr4.4:27497580-27497599 None:intergenic 40.0%
ATCCACAACAAGCACTTCAA+TGG - chr4.4:27496081-27496100 MS.gene059510:intron 40.0%
ATTCCAGTGATCTTTGTGTC+TGG + chr4.4:27496134-27496153 None:intergenic 40.0%
CAAACCATAGTTCCTTTCTG+AGG + chr4.4:27496789-27496808 None:intergenic 40.0%
GCAAATACACAAACATCCAG+TGG + chr4.4:27497592-27497611 None:intergenic 40.0%
TCCACAACAAGCACTTCAAT+GGG - chr4.4:27496082-27496101 MS.gene059510:intron 40.0%
! AGGATATGCATTGACCCAAA+AGG + chr4.4:27496399-27496418 None:intergenic 40.0%
! GGATATGCATTGACCCAAAA+GGG + chr4.4:27496398-27496417 None:intergenic 40.0%
! TCATCAGAGTCACCTTTTGA+TGG + chr4.4:27496617-27496636 None:intergenic 40.0%
! TTTGAAGCAGGCATCACAAA+AGG - chr4.4:27497550-27497569 MS.gene059510:intron 40.0%
!! CCACAACCAGAAAAAGTACT+AGG - chr4.4:27495927-27495946 MS.gene059510:intron 40.0%
!! GCGAAAGCTTTAAGAATCTG+TGG + chr4.4:27496002-27496021 None:intergenic 40.0%
!!! AGAAGCCCTAGTACTTTTTC+TGG + chr4.4:27495936-27495955 None:intergenic 40.0%
!!! CCTAGTACTTTTTCTGGTTG+TGG + chr4.4:27495930-27495949 None:intergenic 40.0%
!!! CTAGCATTTTTTCTCTCTGC+AGG - chr4.4:27495720-27495739 MS.gene059510:CDS 40.0%
AGAATGGACCAACCTCAGAA+AGG - chr4.4:27496774-27496793 MS.gene059510:CDS 45.0%
CCAACCTCAGAAAGGAACTA+TGG - chr4.4:27496782-27496801 MS.gene059510:CDS 45.0%
CTTCCAGACACAAAGATCAC+TGG - chr4.4:27496128-27496147 MS.gene059510:intron 45.0%
GATGATCTCCACGTCTGAAT+TGG + chr4.4:27496024-27496043 None:intergenic 45.0%
GGATCAACCATTCCTTGGTT+AGG + chr4.4:27496479-27496498 None:intergenic 45.0%
TGAAACTACCAGCTGAAGGA+GGG + chr4.4:27496316-27496335 None:intergenic 45.0%
! ACCCATTGAAGTGCTTGTTG+TGG + chr4.4:27496086-27496105 None:intergenic 45.0%
!! CCATAGTTCCTTTCTGAGGT+TGG + chr4.4:27496785-27496804 None:intergenic 45.0%
!! CTTCTGAAACTACCAGCTGA+AGG + chr4.4:27496320-27496339 None:intergenic 45.0%
!! TTAGGGTTTCTGAGACTGGT+TGG - chr4.4:27496591-27496610 MS.gene059510:CDS 45.0%
AGCTTTCGCCAATTCAGACG+TGG - chr4.4:27496013-27496032 MS.gene059510:intron 50.0%
CTCTTACCTCTTCTGCCACT+AGG - chr4.4:27496888-27496907 MS.gene059510:CDS 50.0%
CTCTTCTGCCACTAGGACAA+AGG - chr4.4:27496895-27496914 MS.gene059510:CDS 50.0%
CTGAAACTACCAGCTGAAGG+AGG + chr4.4:27496317-27496336 None:intergenic 50.0%
CTTTGTCCTAGTGGCAGAAG+AGG + chr4.4:27496897-27496916 None:intergenic 50.0%
GAATCTTACCCTCCTTCAGC+TGG - chr4.4:27496305-27496324 MS.gene059510:intron 50.0%
TTCGGACGTGTTGCGTCATT+TGG - chr4.4:27495873-27495892 MS.gene059510:intron 50.0%
! GAGACTGGTTGGCCATCAAA+AGG - chr4.4:27496602-27496621 MS.gene059510:CDS 50.0%
!! GAGGTTAGGGTTTCTGAGAC+TGG - chr4.4:27496587-27496606 MS.gene059510:CDS 50.0%


Chromosome Type Strat End Strand Name
chr4.4 gene 27495678 27497640 27495678 ID=MS.gene059510
chr4.4 mRNA 27495678 27497640 27495678 ID=MS.gene059510.t1;Parent=MS.gene059510
chr4.4 exon 27497577 27497640 27497577 ID=MS.gene059510.t1.exon1;Parent=MS.gene059510.t1
chr4.4 CDS 27497577 27497640 27497577 ID=cds.MS.gene059510.t1;Parent=MS.gene059510.t1
chr4.4 exon 27496402 27497456 27496402 ID=MS.gene059510.t1.exon2;Parent=MS.gene059510.t1
chr4.4 CDS 27496402 27497456 27496402 ID=cds.MS.gene059510.t1;Parent=MS.gene059510.t1
chr4.4 exon 27495678 27495758 27495678 ID=MS.gene059510.t1.exon3;Parent=MS.gene059510.t1
chr4.4 CDS 27495678 27495758 27495678 ID=cds.MS.gene059510.t1;Parent=MS.gene059510.t1
Gene Sequence

>MS.gene059510

ATGGCAACATTTACTCAAAGAATGGCAATATGTTATATTCTTCTAGCATTTTTTCTCTCTGCAGGTACCCTGTTTGTTCTGTTTTTTCACCTATTATTCTTTTCATCATGCATGTTATCTTATCTTTATTGCATTCTATTTTGTAACATTATTGCTAATAGAATTACTGATTATGCAATTACAGATACAGAAATTTTCGGACGTGTTGCGTCATTTGGAATAAACTATGGACAAGTAGCTAACAATTTGCCACAACCAGAAAAAGTACTAGGGCTTCTCAGCACCTTAAAGATCACAAAAACAAGAATCTACGATACGAATCCACAGATTCTTAAAGCTTTCGCCAATTCAGACGTGGAGATCATCGTAACAATTGAAAATCAAATACTAAACCAATTAAATGATCCACAACAAGCACTTCAATGGGTAAATACCAATATCAAACCATATCTTCCAGACACAAAGATCACTGGAATTCAAGTAGGGAATGAAGTGTTCACAGAACTAGACACAACCCTAATTCAATATCTTGTTCCAGCAGTGATCAACATTTACAATGCTCTAGTTCAATTAGGTTTGGACTCAAACATTCACGTGTCAACACCTAGTTCATTAGAAGTTCTTGAAGAATCTTACCCTCCTTCAGCTGGTAGTTTCAGAAGTGAAATCTCTGGAATCATGCATGATTTTTTGAACTTTTTGTCAAAAACAAAATCACCCTTTTGGGTCAATGCATATCCTTATTTTGCTTATAAGAATGACCCTAATCAAATATCTCTAGACTATGTATTATTTAATCCTAACCAAGGAATGGTTGATCCTAATACAAATTTACATTATGATAACATGCTTTATGCTATGGTAGATGCTGTTACATTTGCCATTGCTAAATTAGGTTTTAATGGGATAGAGGTTAGGGTTTCTGAGACTGGTTGGCCATCAAAAGGTGACTCTGATGAAATTGGTGCATCATTGAAAAATGCTGAAATTTATAACAAGAATTTATTGAGGAGACAAATGAAGAATGAAGGAACACCTTTGAATCCTAGAATGAGATTGGAATCATATTTGTTTGCATTATTTAATGAAAATATGAAGAATGGACCAACCTCAGAAAGGAACTATGGTTTGTTTGAGCCTGATGAAAGTATGACTTACAATGTTGGGTTGTCTTCTTTTGCAAAGTCATCAAATCCTTCTACTTCAATTTCTCTTACCTCTTCTGCCACTAGGACAAAGGTAAAAAAAAAAAGAGTAAAGACATTGTTTTTTTTCTTTCTTTTCCAACAATATACAAGTTATTTAGCTTATAGTAAAATTTTGTGATAGAAAATGAAAGTATATAATTTAGATCATGGTTGTTGGTATCTTTGAAGTGCATGCAAATTGTGGGTATATATACTTTCGACATGCAGAAGAATTTAGATTCATTTTGATATATAGATTATGTTTTTTTGGAGTTGAACATTTGTGAAGCAAACAGTTTTTATTTGTTATTTTGATCTATAACTTAGAACCTTGAATTATTTTAAAGTTATTGTATGGTACACCAGCTATGATTTTGTCTATGTCACATGCTTTTTTTAAATATTTTTATTGATCGTGCATCTTATGACAATTTATAAGATTTGAATCATAATTTAAAAAAAAGATGTCTTTTGATACACGATTTGCATTTTGGTTCAATGACCGTGTTTTAGACAAAAGAAAGAAATGTATATCAATTTTTAAAAGAAAGATGTCTAGTGACATATATTAGTCAAATATTTACTTGAAGTATACTATGACTTTTTTGCTATTTTTAGCACTTTATTATATATATTAATTTGATCAACATCAATCTGATTATCTTCTTTTCAAATCAAATTTTACTTTGAAGCAGGCATCACAAAAGGAAAACCAAAGCTTATTCCACTGGATGTTTGTGTATTTGCTGATTTCATCACTTTGTGCCTTTGTTTAA
Protein sequence

>MS.gene059510.t1

MATFTQRMAICYILLAFFLSADTEIFGRVASFGINYGQVANNLPQPEKVLGLLSTLKITKTRIYDTNPQILKAFANSDVEIIVTIENQILNQLNDPQQALQWVNTNIKPYLPDTKITGIQVGNEVFTELDTTLIQYLVPAVINIYNALVQLGLDSNIHVSTPSSLEVLEESYPPSAGSFRSEISGIMHDFLNFLSKTKSPFWVNAYPYFAYKNDPNQISLDYVLFNPNQGMVDPNTNLHYDNMLYAMVDAVTFAIAKLGFNGIEVRVSETGWPSKGDSDEIGASLKNAEIYNKNLLRRQMKNEGTPLNPRMRLESYLFALFNENMKNGPTSERNYGLFEPDESMTYNVGLSSFAKSSNPSTSISLTSSATRTKASQKENQSLFHWMFVYLLISSLCAFV