AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene56478

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene56478.t1 MTR_4g082990 99.375 480 3 0 1 480 1 480 0.0 987
MS.gene56478.t1 MTR_5g043970 86.902 481 62 1 1 480 1 481 0.0 885
MS.gene56478.t1 MTR_7g012950 83.750 480 78 0 1 480 1 480 0.0 855
MS.gene56478.t1 MTR_4g109360 73.362 473 124 2 3 473 4 476 0.0 728
MS.gene56478.t1 MTR_6g014890 52.427 103 47 1 12 114 16 116 1.37e-27 108
MS.gene56478.t1 MTR_6g033430 69.444 72 22 0 64 135 1 72 1.55e-23 94.4
MS.gene56478.t1 MTR_6g014930 50.495 101 27 1 17 117 1 78 5.49e-21 87.4
MS.gene56478.t1 MTR_6g014530 39.535 129 44 2 14 142 8 102 7.44e-19 82.4
MS.gene56478.t1 MTR_6g014840 48.276 87 36 1 1 87 1 78 2.93e-17 77.0
MS.gene56478.t1 MTR_6g014870 43.925 107 30 2 49 155 16 92 2.92e-16 76.3
MS.gene56478.t1 MTR_6g014850 34.463 177 65 4 174 348 71 198 4.18e-16 77.8
MS.gene56478.t1 MTR_6g014850 45.098 102 29 2 1 102 1 75 1.87e-13 70.1
MS.gene56478.t1 MTR_6g014820 41.304 92 18 1 1 92 23 78 2.28e-12 64.3
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene56478.t1 AT3G29360 84.167 480 76 0 1 480 1 480 0.0 856
MS.gene56478.t1 AT3G29360 84.167 480 76 0 1 480 1 480 0.0 856
MS.gene56478.t1 AT5G39320 82.845 478 82 0 1 478 1 478 0.0 851
MS.gene56478.t1 AT5G15490 82.708 480 83 0 1 480 1 480 0.0 847
MS.gene56478.t1 AT1G26570 80.457 481 93 1 1 480 1 481 0.0 816
MS.gene56478.t1 AT3G01010 72.393 163 40 2 318 480 1 158 6.24e-83 253

Find 122 sgRNAs with CRISPR-Local

Find 141 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TGAAAGATATTTATGCTAAT+TGG 0.091386 4.4:+30052902 MS.gene56478:CDS
GTTGGTAAGCTCAGGAAAAT+TGG 0.259126 4.4:+30053684 MS.gene56478:CDS
GTACCCTCAGCAAGAAATTC+AGG 0.280180 4.4:-30052714 None:intergenic
TCTAAGAAGATTGCTGTTCT+TGG 0.281241 4.4:+30053300 MS.gene56478:CDS
TTACTGGAAGCAAGTGATTA+AGG 0.289704 4.4:+30053212 MS.gene56478:CDS
CTGTTCCAACCGTTGATTCT+CGG 0.332760 4.4:-30052357 None:intergenic
TTGAATGCTAGTGTTGGTTT+TGG 0.346083 4.4:+30053114 MS.gene56478:CDS
GAAAGATATTTATGCTAATT+GGG 0.354607 4.4:+30052903 MS.gene56478:CDS
TGTTAAATCAGCCGCTTTGC+CGG 0.358742 4.4:-30052538 None:intergenic
AGAGGATTCTGACTCATAAT+AGG 0.360802 4.4:+30052661 MS.gene56478:CDS
ATTGCTTCAGCTGTTCTAAC+AGG 0.364317 4.4:-30052636 None:intergenic
AAGCGGCTGATTTAACATAC+TGG 0.368249 4.4:+30052544 MS.gene56478:CDS
TTCATGTGGTAGCAAATTAC+TGG 0.378490 4.4:+30053196 MS.gene56478:CDS
TGCTTTATCGAAGTTGGACT+TGG 0.380856 4.4:-30053504 None:intergenic
CAAGGGTTTGTTGGGAGATA+AGG 0.381297 4.4:+30053380 MS.gene56478:CDS
GCCACTTGCTTTATCGAAGT+TGG 0.388159 4.4:-30053510 None:intergenic
AAAGCAAGTGGCTGTGGTTT+GGG 0.389789 4.4:+30053521 MS.gene56478:CDS
GTCTGCTGAGCTCTCTAAAC+TGG 0.391926 4.4:+30052960 MS.gene56478:CDS
ACCACGGTTACTGCAATCTT+AGG 0.392452 4.4:-30052321 None:intergenic
GGATTTATTGTTTATTCCAT+TGG 0.392509 4.4:+30053705 MS.gene56478:CDS
ATTTGATGGTAGGAATGTTA+TGG 0.395140 4.4:+30053659 MS.gene56478:CDS
TAAAGCAAGTGGCTGTGGTT+TGG 0.402728 4.4:+30053520 MS.gene56478:CDS
CCACTTCAGTGCTGAAGAAA+AGG 0.417004 4.4:-30052443 None:intergenic
CGATGTGTGCAAGGGTTTGT+TGG 0.421184 4.4:+30053371 MS.gene56478:CDS
AGAGATTCTCTGTGCCAAAA+AGG 0.424500 4.4:-30052992 None:intergenic
TGTGGTTTGGGATGCTTATG+AGG 0.427030 4.4:+30053533 MS.gene56478:CDS
ATCCTTTATGATCTGATCTT+CGG 0.439079 4.4:-30053433 None:intergenic
AAAATTTGTTGCATCGGAGC+TGG 0.445503 4.4:+30052255 MS.gene56478:CDS
AGCCCGGATCGTGTCCTAAT+CGG 0.446831 4.4:+30052838 MS.gene56478:CDS
CAACAACTCGGTTCACAAAC+CGG 0.448174 4.4:-30053254 None:intergenic
GTCTATATTTGCGAGTGTAA+TGG 0.448773 4.4:+30053171 MS.gene56478:CDS
GTGTCTCCCTACCTCCGATT+AGG 0.449487 4.4:-30052852 None:intergenic
TGCAATCTTAGGACACTTCA+AGG 0.467490 4.4:-30052310 None:intergenic
GATCGCCGATGTTTCTAAAT+CGG 0.469822 4.4:+30052584 MS.gene56478:CDS
TGGTGGCTCTTGCTTTCAGA+AGG 0.474973 4.4:+30053134 MS.gene56478:CDS
GGACACGATCCGGGCTGAAA+AGG 0.485556 4.4:-30052831 None:intergenic
ATCGGAACAGACTCGAGGAT+TGG 0.486245 4.4:+30053081 MS.gene56478:CDS
TTATTCCATTGGTAAGCCAC+TGG 0.486901 4.4:+30053716 MS.gene56478:CDS
AAGCCGTGGTGAAGGAGTGC+AGG 0.486980 4.4:+30052412 MS.gene56478:CDS
TTCGATAAAGCAAGTGGCTG+TGG 0.488251 4.4:+30053515 MS.gene56478:CDS
TACCGAAGATCAGATCATAA+AGG 0.492354 4.4:+30053431 MS.gene56478:CDS
GATGTGTGCAAGGGTTTGTT+GGG 0.501277 4.4:+30053372 MS.gene56478:CDS
CCCTTGCACACATCGATAGC+CGG 0.502325 4.4:-30053363 None:intergenic
TACCCTCAGCAAGAAATTCA+GGG 0.503132 4.4:-30052713 None:intergenic
TTCACCACGGCTTCAAGACC+AGG 0.505299 4.4:-30052402 None:intergenic
AGCAACTGGTGCTGATATCT+CGG 0.508415 4.4:+30053044 MS.gene56478:CDS
AAAACTCATGGTCTTGGAGC+CGG 0.509641 4.4:+30052519 MS.gene56478:CDS
TCTTGGTTTCTCTTTCAAGA+AGG 0.511984 4.4:+30053317 MS.gene56478:CDS
TTTATCGAAGTTGGACTTGG+TGG 0.513987 4.4:-30053501 None:intergenic
TCAGAAGGATATAATGAACT+TGG 0.517285 4.4:+30053149 MS.gene56478:CDS
GGTAGGAATGTTATGGATGT+TGG 0.519091 4.4:+30053666 MS.gene56478:CDS
ATGGTGAAAATTTGTTGCAT+CGG 0.519668 4.4:+30052249 MS.gene56478:CDS
TTATGGATGTTGGTAAGCTC+AGG 0.521362 4.4:+30053676 MS.gene56478:CDS
GGAGACACCAGAAGGACAAA+AGG 0.523750 4.4:+30052867 MS.gene56478:CDS
TTGGTAAGCCACTGGATCCA+TGG 0.524811 4.4:+30053724 MS.gene56478:CDS
TTATGCTAATTGGGTCCCTA+AGG 0.524851 4.4:+30052912 MS.gene56478:CDS
AGAAGGACACAGGCGATACA+AGG 0.525027 4.4:+30053334 MS.gene56478:CDS
CTTGGTGGTTGAAGATGAGC+AGG 0.525970 4.4:-30053486 None:intergenic
ACCGGCTATCGATGTGTGCA+AGG 0.530309 4.4:+30053362 MS.gene56478:CDS
TGGTCTTGAAGCCGTGGTGA+AGG 0.539097 4.4:+30052404 MS.gene56478:CDS
CAAGGCAATCACAGCCATTG+TGG 0.541163 4.4:-30052292 None:intergenic
TTCTAAATCGGACAAAATTG+TGG 0.542076 4.4:+30052596 MS.gene56478:CDS
AACCCTGAATTTCTTGCTGA+GGG 0.543013 4.4:+30052711 MS.gene56478:CDS
ATTTCAGCACCGAGAATCAA+CGG 0.543330 4.4:+30052348 MS.gene56478:CDS
TCAAGACCAGGCTCGTAGAT+CGG 0.543878 4.4:-30052390 None:intergenic
TTAATGACTATCAGAAAAGC+CGG 0.546119 4.4:+30053235 MS.gene56478:CDS
TCGGAGGTATCACACTCAAT+CGG 0.548115 4.4:+30053063 MS.gene56478:CDS
GAGCTCAGCAGACCACAGAT+TGG 0.552085 4.4:-30052950 None:intergenic
AAGGCAATCACAGCCATTGT+GGG 0.553236 4.4:-30052291 None:intergenic
TTCTCTTTCAAGAAGGACAC+AGG 0.554715 4.4:+30053324 MS.gene56478:CDS
TCCTTGAGCCATGGATCCAG+TGG 0.560633 4.4:-30053732 None:intergenic
TGGATCCAGTGGCTTACCAA+TGG 0.562663 4.4:-30053721 None:intergenic
CCTCCGATTAGGACACGATC+CGG 0.564937 4.4:-30052841 None:intergenic
TCGTGTCCTAATCGGAGGTA+GGG 0.567068 4.4:+30052846 MS.gene56478:CDS
GCAGGCATGTCCTTGAGCCA+TGG 0.569217 4.4:-30053741 None:intergenic
AGGCGATACAAGGGAAGCAC+CGG 0.573278 4.4:+30053344 MS.gene56478:CDS
TGCATCGGAGCTGGATATGT+TGG 0.574012 4.4:+30052264 MS.gene56478:CDS
CGAGCCTGGTCTTGAAGCCG+TGG 0.575665 4.4:+30052398 MS.gene56478:CDS
TGGTGAAGGAGTGCAGGGGA+AGG 0.576100 4.4:+30052418 MS.gene56478:CDS
CACCTACCGATCTACGAGCC+TGG 0.576813 4.4:+30052384 MS.gene56478:CDS
AGCGGCTGATTTAACATACT+GGG 0.577833 4.4:+30052545 MS.gene56478:CDS
AAACCCTGAATTTCTTGCTG+AGG 0.578960 4.4:+30052710 MS.gene56478:CDS
ATCTGATCTTCGGTAACTTG+AGG 0.579881 4.4:-30053423 None:intergenic
TCAGCACTATGTGAAGCAAC+TGG 0.581495 4.4:+30053030 MS.gene56478:CDS
CAGCACCGAGAATCAACGGT+TGG 0.582970 4.4:+30052352 MS.gene56478:CDS
ACACTAGCATTCAAGAACTT+AGG 0.584911 4.4:-30053105 None:intergenic
AAGTTCTTGAATGCTAGTGT+TGG 0.587122 4.4:+30053108 MS.gene56478:CDS
GTTTGTTGGGAGATAAGGCA+AGG 0.587768 4.4:+30053385 MS.gene56478:CDS
AGAAGCAACCGTGTTGAACA+TGG 0.590789 4.4:-30053280 None:intergenic
GCCACTGGATCCATGGCTCA+AGG 0.597097 4.4:+30053731 MS.gene56478:CDS
TCCAACTTCGATAAAGCAAG+TGG 0.600253 4.4:+30053509 MS.gene56478:CDS
TTTGTCCGATTTAGAAACAT+CGG 0.602624 4.4:-30052589 None:intergenic
ATCGGAGCTGGATATGTTGG+TGG 0.603146 4.4:+30052267 MS.gene56478:CDS
AGCCGTGGTGAAGGAGTGCA+GGG 0.608786 4.4:+30052413 MS.gene56478:CDS
AAATCATTTGCACCAATCTG+TGG 0.612903 4.4:+30052938 MS.gene56478:CDS
ATCGTGTCCTAATCGGAGGT+AGG 0.615001 4.4:+30052845 MS.gene56478:CDS
GCTCAAGGACATGCCTGCAG+TGG 0.625363 4.4:+30053746 MS.gene56478:CDS
TCCCCTGCACTCCTTCACCA+CGG 0.628514 4.4:-30052415 None:intergenic
TGAACATGGACGCAACAACT+CGG 0.630690 4.4:-30053266 None:intergenic
CCGGCTATCGATGTGTGCAA+GGG 0.631110 4.4:+30053363 MS.gene56478:CDS
CTCCGATTAGGACACGATCC+GGG 0.633554 4.4:-30052840 None:intergenic
GACCAGGCTCGTAGATCGGT+AGG 0.634392 4.4:-30052386 None:intergenic
TGTTGCGTCCATGTTCAACA+CGG 0.638044 4.4:+30053272 MS.gene56478:CDS
GAAGGACACAGGCGATACAA+GGG 0.639231 4.4:+30053335 MS.gene56478:CDS
GAACAGCTGAAGCAATTGAG+AGG 0.641777 4.4:+30052643 MS.gene56478:CDS
GGAGGTAGGGAGACACCAGA+AGG 0.655737 4.4:+30052859 MS.gene56478:CDS
CCGGATCGTGTCCTAATCGG+AGG 0.664959 4.4:+30052841 MS.gene56478:CDS
TCCTAAGATTGCAGTAACCG+TGG 0.667449 4.4:+30052320 MS.gene56478:CDS
CGAGTGTAATGGTCTTCATG+TGG 0.667527 4.4:+30053182 MS.gene56478:CDS
ATATGTTGGTGGTCCCACAA+TGG 0.680415 4.4:+30052278 MS.gene56478:CDS
TGGTCTTGGAGCCGGCAAAG+CGG 0.691470 4.4:+30052527 MS.gene56478:CDS
ACTCAATCGGAACAGACTCG+AGG 0.702881 4.4:+30053076 MS.gene56478:CDS
AACTGGTGCTGATATCTCGG+AGG 0.704506 4.4:+30053047 MS.gene56478:CDS
CATACTGGGAGAGTTCCGCT+CGG 0.706063 4.4:+30052559 MS.gene56478:CDS
GTGTTTGCATTATGACTGAG+TGG 0.706540 4.4:+30053574 MS.gene56478:CDS
TGTTTGCATTATGACTGAGT+GGG 0.707780 4.4:+30053575 MS.gene56478:CDS
CGGTGCTGAAATATCGACCA+CGG 0.712301 4.4:-30052337 None:intergenic
CAGGCTCGTAGATCGGTAGG+TGG 0.714440 4.4:-30052383 None:intergenic
GCCGTGGTGAAGGAGTGCAG+GGG 0.744462 4.4:+30052414 MS.gene56478:CDS
GGTTTGGGATGCTTATGAGG+CGG 0.748746 4.4:+30053536 MS.gene56478:CDS
AACATCGGCGATCATCCGAG+CGG 0.754134 4.4:-30052574 None:intergenic
GAGGCGGTGAAAGATGCACA+CGG 0.755151 4.4:+30053552 MS.gene56478:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! GAAAGATATTTATGCTAATT+GGG + chr4.4:30052903-30052922 MS.gene56478:CDS 20.0%
!! TGAAAGATATTTATGCTAAT+TGG + chr4.4:30052902-30052921 MS.gene56478:CDS 20.0%
!!! AATTTTATCTGTAATAGGTA+CGG + chr4.4:30052798-30052817 MS.gene56478:intron 20.0%
!!! GCATATTGTCATAAATTTTT+TGG - chr4.4:30053623-30053642 None:intergenic 20.0%
! AATTCCTGAAATAGTTAGTT+AGG + chr4.4:30052737-30052756 MS.gene56478:intron 25.0%
! ATAACCTAACTAACTATTTC+AGG - chr4.4:30052744-30052763 None:intergenic 25.0%
! GGATTTATTGTTTATTCCAT+TGG + chr4.4:30053705-30053724 MS.gene56478:CDS 25.0%
! GTACCTATTACAGATAAAAT+TGG - chr4.4:30052799-30052818 None:intergenic 25.0%
!! GTACCAATTTTATCTGTAAT+AGG + chr4.4:30052793-30052812 MS.gene56478:intron 25.0%
ATCCTTTATGATCTGATCTT+CGG - chr4.4:30053436-30053455 None:intergenic 30.0%
CTACCATCAAATAAAAATGC+TGG - chr4.4:30053651-30053670 None:intergenic 30.0%
TCAGAAGGATATAATGAACT+TGG + chr4.4:30053149-30053168 MS.gene56478:CDS 30.0%
TTAATGACTATCAGAAAAGC+CGG + chr4.4:30053235-30053254 MS.gene56478:CDS 30.0%
TTCTAAATCGGACAAAATTG+TGG + chr4.4:30052596-30052615 MS.gene56478:CDS 30.0%
TTTGTCCGATTTAGAAACAT+CGG - chr4.4:30052592-30052611 None:intergenic 30.0%
! ATTTGATGGTAGGAATGTTA+TGG + chr4.4:30053659-30053678 MS.gene56478:CDS 30.0%
! TGCAAATGATTTTCTCCTTA+GGG - chr4.4:30052930-30052949 None:intergenic 30.0%
!! AAGCCAGCATTTTTATTTGA+TGG + chr4.4:30053645-30053664 MS.gene56478:CDS 30.0%
!! ATGGTGAAAATTTGTTGCAT+CGG + chr4.4:30052249-30052268 MS.gene56478:CDS 30.0%
!! CAGCATTTTTATTTGATGGT+AGG + chr4.4:30053649-30053668 MS.gene56478:CDS 30.0%
AAATCATTTGCACCAATCTG+TGG + chr4.4:30052938-30052957 MS.gene56478:CDS 35.0%
AACACACCAACAAAAACTCA+TGG + chr4.4:30052507-30052526 MS.gene56478:CDS 35.0%
AAGTTCTTGAATGCTAGTGT+TGG + chr4.4:30053108-30053127 MS.gene56478:CDS 35.0%
ACACTAGCATTCAAGAACTT+AGG - chr4.4:30053108-30053127 None:intergenic 35.0%
AGAGGATTCTGACTCATAAT+AGG + chr4.4:30052661-30052680 MS.gene56478:CDS 35.0%
GTCTATATTTGCGAGTGTAA+TGG + chr4.4:30053171-30053190 MS.gene56478:CDS 35.0%
TACCGAAGATCAGATCATAA+AGG + chr4.4:30053431-30053450 MS.gene56478:CDS 35.0%
TCTTGGTTTCTCTTTCAAGA+AGG + chr4.4:30053317-30053336 MS.gene56478:CDS 35.0%
TGTTTGCATTATGACTGAGT+GGG + chr4.4:30053575-30053594 MS.gene56478:CDS 35.0%
TTACTGGAAGCAAGTGATTA+AGG + chr4.4:30053212-30053231 MS.gene56478:CDS 35.0%
TTCATGTGGTAGCAAATTAC+TGG + chr4.4:30053196-30053215 MS.gene56478:CDS 35.0%
! GTGCAAATGATTTTCTCCTT+AGG - chr4.4:30052931-30052950 None:intergenic 35.0%
! TCTAAGAAGATTGCTGTTCT+TGG + chr4.4:30053300-30053319 MS.gene56478:CDS 35.0%
!!! TTGAATGCTAGTGTTGGTTT+TGG + chr4.4:30053114-30053133 MS.gene56478:CDS 35.0%
AAACCCTGAATTTCTTGCTG+AGG + chr4.4:30052710-30052729 MS.gene56478:CDS 40.0%
AACCCTGAATTTCTTGCTGA+GGG + chr4.4:30052711-30052730 MS.gene56478:CDS 40.0%
AGAGATTCTCTGTGCCAAAA+AGG - chr4.4:30052995-30053014 None:intergenic 40.0%
ATCTGATCTTCGGTAACTTG+AGG - chr4.4:30053426-30053445 None:intergenic 40.0%
ATTGCTTCAGCTGTTCTAAC+AGG - chr4.4:30052639-30052658 None:intergenic 40.0%
CCAACAAAAACTCATGGTCT+TGG + chr4.4:30052513-30052532 MS.gene56478:CDS 40.0%
GGTAGGAATGTTATGGATGT+TGG + chr4.4:30053666-30053685 MS.gene56478:CDS 40.0%
GTGTTTGCATTATGACTGAG+TGG + chr4.4:30053574-30053593 MS.gene56478:CDS 40.0%
TACCCTCAGCAAGAAATTCA+GGG - chr4.4:30052716-30052735 None:intergenic 40.0%
TCCAACTTCGATAAAGCAAG+TGG + chr4.4:30053509-30053528 MS.gene56478:CDS 40.0%
TGCAATCTTAGGACACTTCA+AGG - chr4.4:30052313-30052332 None:intergenic 40.0%
TGCTTTATCGAAGTTGGACT+TGG - chr4.4:30053507-30053526 None:intergenic 40.0%
TTATGCTAATTGGGTCCCTA+AGG + chr4.4:30052912-30052931 MS.gene56478:CDS 40.0%
TTATTCCATTGGTAAGCCAC+TGG + chr4.4:30053716-30053735 MS.gene56478:CDS 40.0%
TTCTCTTTCAAGAAGGACAC+AGG + chr4.4:30053324-30053343 MS.gene56478:CDS 40.0%
TTTATCGAAGTTGGACTTGG+TGG - chr4.4:30053504-30053523 None:intergenic 40.0%
! AAGCGGCTGATTTAACATAC+TGG + chr4.4:30052544-30052563 MS.gene56478:CDS 40.0%
! AATCAAAGACCTTTTCAGCC+CGG + chr4.4:30052822-30052841 MS.gene56478:CDS 40.0%
! AGCGGCTGATTTAACATACT+GGG + chr4.4:30052545-30052564 MS.gene56478:CDS 40.0%
! ATTTCAGCACCGAGAATCAA+CGG + chr4.4:30052348-30052367 MS.gene56478:CDS 40.0%
! CCAAGACCATGAGTTTTTGT+TGG - chr4.4:30052516-30052535 None:intergenic 40.0%
! GATCGCCGATGTTTCTAAAT+CGG + chr4.4:30052584-30052603 MS.gene56478:CDS 40.0%
! GTTGGTAAGCTCAGGAAAAT+TGG + chr4.4:30053684-30053703 MS.gene56478:CDS 40.0%
! TCTATAGCCTTTTGTCCTTC+TGG - chr4.4:30052877-30052896 None:intergenic 40.0%
! TTATGGATGTTGGTAAGCTC+AGG + chr4.4:30053676-30053695 MS.gene56478:CDS 40.0%
!! AAAATTTGTTGCATCGGAGC+TGG + chr4.4:30052255-30052274 MS.gene56478:CDS 40.0%
!!! AATGCTAGTGTTGGTTTTGG+TGG + chr4.4:30053117-30053136 MS.gene56478:CDS 40.0%
AAAGCAAGTGGCTGTGGTTT+GGG + chr4.4:30053521-30053540 MS.gene56478:CDS 45.0%
AAGGCAATCACAGCCATTGT+GGG - chr4.4:30052294-30052313 None:intergenic 45.0%
ACCACGGTTACTGCAATCTT+AGG - chr4.4:30052324-30052343 None:intergenic 45.0%
AGAAGCAACCGTGTTGAACA+TGG - chr4.4:30053283-30053302 None:intergenic 45.0%
CAACAACTCGGTTCACAAAC+CGG - chr4.4:30053257-30053276 None:intergenic 45.0%
CGAGTGTAATGGTCTTCATG+TGG + chr4.4:30053182-30053201 MS.gene56478:CDS 45.0%
GAACAGCTGAAGCAATTGAG+AGG + chr4.4:30052643-30052662 MS.gene56478:CDS 45.0%
GCCACTTGCTTTATCGAAGT+TGG - chr4.4:30053513-30053532 None:intergenic 45.0%
GTACCCTCAGCAAGAAATTC+AGG - chr4.4:30052717-30052736 None:intergenic 45.0%
TAAAGCAAGTGGCTGTGGTT+TGG + chr4.4:30053520-30053539 MS.gene56478:CDS 45.0%
TCAGCACTATGTGAAGCAAC+TGG + chr4.4:30053030-30053049 MS.gene56478:CDS 45.0%
TCCTAAGATTGCAGTAACCG+TGG + chr4.4:30052320-30052339 MS.gene56478:CDS 45.0%
TGAACATGGACGCAACAACT+CGG - chr4.4:30053269-30053288 None:intergenic 45.0%
TGTTGCGTCCATGTTCAACA+CGG + chr4.4:30053272-30053291 MS.gene56478:CDS 45.0%
TTCGATAAAGCAAGTGGCTG+TGG + chr4.4:30053515-30053534 MS.gene56478:CDS 45.0%
! AAAACTCATGGTCTTGGAGC+CGG + chr4.4:30052519-30052538 MS.gene56478:CDS 45.0%
! CCACTTCAGTGCTGAAGAAA+AGG - chr4.4:30052446-30052465 None:intergenic 45.0%
! GATGTGTGCAAGGGTTTGTT+GGG + chr4.4:30053372-30053391 MS.gene56478:CDS 45.0%
! GTTTGTTGGGAGATAAGGCA+AGG + chr4.4:30053385-30053404 MS.gene56478:CDS 45.0%
! TGTGGTTTGGGATGCTTATG+AGG + chr4.4:30053533-30053552 MS.gene56478:CDS 45.0%
! TGTTAAATCAGCCGCTTTGC+CGG - chr4.4:30052541-30052560 None:intergenic 45.0%
!! AGCAACTGGTGCTGATATCT+CGG + chr4.4:30053044-30053063 MS.gene56478:CDS 45.0%
!! ATATGTTGGTGGTCCCACAA+TGG + chr4.4:30052278-30052297 MS.gene56478:CDS 45.0%
!! CAAGGGTTTGTTGGGAGATA+AGG + chr4.4:30053380-30053399 MS.gene56478:CDS 45.0%
!! CCTTTTCTTCAGCACTGAAG+TGG + chr4.4:30052443-30052462 MS.gene56478:CDS 45.0%
!! CTGTTCCAACCGTTGATTCT+CGG - chr4.4:30052360-30052379 None:intergenic 45.0%
!! TCGGAGGTATCACACTCAAT+CGG + chr4.4:30053063-30053082 MS.gene56478:CDS 45.0%
ACTCAATCGGAACAGACTCG+AGG + chr4.4:30053076-30053095 MS.gene56478:CDS 50.0%
AGAAGGACACAGGCGATACA+AGG + chr4.4:30053334-30053353 MS.gene56478:CDS 50.0%
ATCGGAACAGACTCGAGGAT+TGG + chr4.4:30053081-30053100 MS.gene56478:CDS 50.0%
ATCGGAGCTGGATATGTTGG+TGG + chr4.4:30052267-30052286 MS.gene56478:CDS 50.0%
ATCGTGTCCTAATCGGAGGT+AGG + chr4.4:30052845-30052864 MS.gene56478:CDS 50.0%
CAAGGCAATCACAGCCATTG+TGG - chr4.4:30052295-30052314 None:intergenic 50.0%
CGATGTGTGCAAGGGTTTGT+TGG + chr4.4:30053371-30053390 MS.gene56478:CDS 50.0%
GAAGGACACAGGCGATACAA+GGG + chr4.4:30053335-30053354 MS.gene56478:CDS 50.0%
GGAGACACCAGAAGGACAAA+AGG + chr4.4:30052867-30052886 MS.gene56478:CDS 50.0%
GTCTGCTGAGCTCTCTAAAC+TGG + chr4.4:30052960-30052979 MS.gene56478:CDS 50.0%
TCAAGACCAGGCTCGTAGAT+CGG - chr4.4:30052393-30052412 None:intergenic 50.0%
TCGTGTCCTAATCGGAGGTA+GGG + chr4.4:30052846-30052865 MS.gene56478:CDS 50.0%
TGCATCGGAGCTGGATATGT+TGG + chr4.4:30052264-30052283 MS.gene56478:CDS 50.0%
TGGATCCAGTGGCTTACCAA+TGG - chr4.4:30053724-30053743 None:intergenic 50.0%
TGGTGGCTCTTGCTTTCAGA+AGG + chr4.4:30053134-30053153 MS.gene56478:CDS 50.0%
TTGGTAAGCCACTGGATCCA+TGG + chr4.4:30053724-30053743 MS.gene56478:CDS 50.0%
! ACTGGCTGCTAACGCCTTTT+TGG + chr4.4:30052978-30052997 MS.gene56478:CDS 50.0%
! CGGTGCTGAAATATCGACCA+CGG - chr4.4:30052340-30052359 None:intergenic 50.0%
! CTTGGTGGTTGAAGATGAGC+AGG - chr4.4:30053489-30053508 None:intergenic 50.0%
! GGTTTGGGATGCTTATGAGG+CGG + chr4.4:30053536-30053555 MS.gene56478:CDS 50.0%
!! AACTGGTGCTGATATCTCGG+AGG + chr4.4:30053047-30053066 MS.gene56478:CDS 50.0%
AACATCGGCGATCATCCGAG+CGG - chr4.4:30052577-30052596 None:intergenic 55.0%
ACCGGCTATCGATGTGTGCA+AGG + chr4.4:30053362-30053381 MS.gene56478:CDS 55.0%
AGCCCGGATCGTGTCCTAAT+CGG + chr4.4:30052838-30052857 MS.gene56478:CDS 55.0%
CATACTGGGAGAGTTCCGCT+CGG + chr4.4:30052559-30052578 MS.gene56478:CDS 55.0%
CCCTTGCACACATCGATAGC+CGG - chr4.4:30053366-30053385 None:intergenic 55.0%
CCGGCTATCGATGTGTGCAA+GGG + chr4.4:30053363-30053382 MS.gene56478:CDS 55.0%
CCTCCGATTAGGACACGATC+CGG - chr4.4:30052844-30052863 None:intergenic 55.0%
CTCCGATTAGGACACGATCC+GGG - chr4.4:30052843-30052862 None:intergenic 55.0%
GAGCTCAGCAGACCACAGAT+TGG - chr4.4:30052953-30052972 None:intergenic 55.0%
GAGGCGGTGAAAGATGCACA+CGG + chr4.4:30053552-30053571 MS.gene56478:CDS 55.0%
GTGTCTCCCTACCTCCGATT+AGG - chr4.4:30052855-30052874 None:intergenic 55.0%
TCCTTGAGCCATGGATCCAG+TGG - chr4.4:30053735-30053754 None:intergenic 55.0%
TTCACCACGGCTTCAAGACC+AGG - chr4.4:30052405-30052424 None:intergenic 55.0%
! AGGCGATACAAGGGAAGCAC+CGG + chr4.4:30053344-30053363 MS.gene56478:CDS 55.0%
! CAGCACCGAGAATCAACGGT+TGG + chr4.4:30052352-30052371 MS.gene56478:CDS 55.0%
! TGGTCTTGAAGCCGTGGTGA+AGG + chr4.4:30052404-30052423 MS.gene56478:CDS 55.0%
CACCTACCGATCTACGAGCC+TGG + chr4.4:30052384-30052403 MS.gene56478:CDS 60.0%
CAGGCTCGTAGATCGGTAGG+TGG - chr4.4:30052386-30052405 None:intergenic 60.0%
CCGGATCGTGTCCTAATCGG+AGG + chr4.4:30052841-30052860 MS.gene56478:CDS 60.0%
GACCAGGCTCGTAGATCGGT+AGG - chr4.4:30052389-30052408 None:intergenic 60.0%
GCAGGCATGTCCTTGAGCCA+TGG - chr4.4:30053744-30053763 None:intergenic 60.0%
GCCACTGGATCCATGGCTCA+AGG + chr4.4:30053731-30053750 MS.gene56478:CDS 60.0%
GCTCAAGGACATGCCTGCAG+TGG + chr4.4:30053746-30053765 MS.gene56478:CDS 60.0%
GGACACGATCCGGGCTGAAA+AGG - chr4.4:30052834-30052853 None:intergenic 60.0%
GGAGGTAGGGAGACACCAGA+AGG + chr4.4:30052859-30052878 MS.gene56478:CDS 60.0%
TCCCCTGCACTCCTTCACCA+CGG - chr4.4:30052418-30052437 None:intergenic 60.0%
! AAGCCGTGGTGAAGGAGTGC+AGG + chr4.4:30052412-30052431 MS.gene56478:CDS 60.0%
! AGCCGTGGTGAAGGAGTGCA+GGG + chr4.4:30052413-30052432 MS.gene56478:CDS 60.0%
! TGGTCTTGGAGCCGGCAAAG+CGG + chr4.4:30052527-30052546 MS.gene56478:CDS 60.0%
! TGGTGAAGGAGTGCAGGGGA+AGG + chr4.4:30052418-30052437 MS.gene56478:CDS 60.0%
! GCCGTGGTGAAGGAGTGCAG+GGG + chr4.4:30052414-30052433 MS.gene56478:CDS 65.0%
!! CGAGCCTGGTCTTGAAGCCG+TGG + chr4.4:30052398-30052417 MS.gene56478:CDS 65.0%


Chromosome Type Strat End Strand Name
chr4.4 gene 30052249 30053773 30052249 ID=MS.gene56478
chr4.4 mRNA 30052249 30053773 30052249 ID=MS.gene56478.t1;Parent=MS.gene56478
chr4.4 exon 30052249 30052732 30052249 ID=MS.gene56478.t1.exon1;Parent=MS.gene56478.t1
chr4.4 CDS 30052249 30052732 30052249 ID=cds.MS.gene56478.t1;Parent=MS.gene56478.t1
chr4.4 exon 30052815 30053773 30052815 ID=MS.gene56478.t1.exon2;Parent=MS.gene56478.t1
chr4.4 CDS 30052815 30053773 30052815 ID=cds.MS.gene56478.t1;Parent=MS.gene56478.t1
Gene Sequence

>MS.gene56478

ATGGTGAAAATTTGTTGCATCGGAGCTGGATATGTTGGTGGTCCCACAATGGCTGTGATTGCCTTGAAGTGTCCTAAGATTGCAGTAACCGTGGTCGATATTTCAGCACCGAGAATCAACGGTTGGAACAGCGACCACCTACCGATCTACGAGCCTGGTCTTGAAGCCGTGGTGAAGGAGTGCAGGGGAAGGAACCTTTTCTTCAGCACTGAAGTGGAGAAACATGTTGCAGAAGCTGATATAGTCTTTGTATCTGTGAACACACCAACAAAAACTCATGGTCTTGGAGCCGGCAAAGCGGCTGATTTAACATACTGGGAGAGTTCCGCTCGGATGATCGCCGATGTTTCTAAATCGGACAAAATTGTGGTTGAAAAATCAACTGTACCTGTTAGAACAGCTGAAGCAATTGAGAGGATTCTGACTCATAATAGGAAAAAAATCAATTTCACTATTTTGTCAAACCCTGAATTTCTTGCTGAGGGTACAATTCCTGAAATAGTTAGTTAGGTTATTTAATTTTGTTATCTTCGTAACTAATTATGTACCAATTTTATCTGTAATAGGTACGGCAATCAAAGACCTTTTCAGCCCGGATCGTGTCCTAATCGGAGGTAGGGAGACACCAGAAGGACAAAAGGCTATAGAAACATTGAAAGATATTTATGCTAATTGGGTCCCTAAGGAGAAAATCATTTGCACCAATCTGTGGTCTGCTGAGCTCTCTAAACTGGCTGCTAACGCCTTTTTGGCACAGAGAATCTCTTCTGTGAATGCTATGTCAGCACTATGTGAAGCAACTGGTGCTGATATCTCGGAGGTATCACACTCAATCGGAACAGACTCGAGGATTGGACCTAAGTTCTTGAATGCTAGTGTTGGTTTTGGTGGCTCTTGCTTTCAGAAGGATATAATGAACTTGGTCTATATTTGCGAGTGTAATGGTCTTCATGTGGTAGCAAATTACTGGAAGCAAGTGATTAAGGTTAATGACTATCAGAAAAGCCGGTTTGTGAACCGAGTTGTTGCGTCCATGTTCAACACGGTTGCTTCTAAGAAGATTGCTGTTCTTGGTTTCTCTTTCAAGAAGGACACAGGCGATACAAGGGAAGCACCGGCTATCGATGTGTGCAAGGGTTTGTTGGGAGATAAGGCAAGGTTGAGCATATACGATCCTCAAGTTACCGAAGATCAGATCATAAAGGATCTGTCGATGAAGAAATTCGATAAAGAACAGCCTGCTCATCTTCAACCACCAAGTCCAACTTCGATAAAGCAAGTGGCTGTGGTTTGGGATGCTTATGAGGCGGTGAAAGATGCACACGGTGTTTGCATTATGACTGAGTGGGACGAGTTTAAGAAACTCGATTACCAAAAAATTTATGACAATATGCAGAAGCCAGCATTTTTATTTGATGGTAGGAATGTTATGGATGTTGGTAAGCTCAGGAAAATTGGATTTATTGTTTATTCCATTGGTAAGCCACTGGATCCATGGCTCAAGGACATGCCTGCAGTGGCATAA
Protein sequence

>MS.gene56478.t1

MVKICCIGAGYVGGPTMAVIALKCPKIAVTVVDISAPRINGWNSDHLPIYEPGLEAVVKECRGRNLFFSTEVEKHVAEADIVFVSVNTPTKTHGLGAGKAADLTYWESSARMIADVSKSDKIVVEKSTVPVRTAEAIERILTHNRKKINFTILSNPEFLAEGTAIKDLFSPDRVLIGGRETPEGQKAIETLKDIYANWVPKEKIICTNLWSAELSKLAANAFLAQRISSVNAMSALCEATGADISEVSHSIGTDSRIGPKFLNASVGFGGSCFQKDIMNLVYICECNGLHVVANYWKQVIKVNDYQKSRFVNRVVASMFNTVASKKIAVLGFSFKKDTGDTREAPAIDVCKGLLGDKARLSIYDPQVTEDQIIKDLSMKKFDKEQPAHLQPPSPTSIKQVAVVWDAYEAVKDAHGVCIMTEWDEFKKLDYQKIYDNMQKPAFLFDGRNVMDVGKLRKIGFIVYSIGKPLDPWLKDMPAVA