AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene56480

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene56480.t1 MTR_4g082990 99.375 480 3 0 1 480 1 480 0.0 987
MS.gene56480.t1 MTR_5g043970 86.902 481 62 1 1 480 1 481 0.0 885
MS.gene56480.t1 MTR_7g012950 83.750 480 78 0 1 480 1 480 0.0 855
MS.gene56480.t1 MTR_4g109360 73.362 473 124 2 3 473 4 476 0.0 728
MS.gene56480.t1 MTR_6g014890 52.427 103 47 1 12 114 16 116 1.37e-27 108
MS.gene56480.t1 MTR_6g033430 69.444 72 22 0 64 135 1 72 1.55e-23 94.4
MS.gene56480.t1 MTR_6g014930 50.495 101 27 1 17 117 1 78 5.49e-21 87.4
MS.gene56480.t1 MTR_6g014530 39.535 129 44 2 14 142 8 102 7.44e-19 82.4
MS.gene56480.t1 MTR_6g014840 48.276 87 36 1 1 87 1 78 2.93e-17 77.0
MS.gene56480.t1 MTR_6g014870 43.925 107 30 2 49 155 16 92 2.92e-16 76.3
MS.gene56480.t1 MTR_6g014850 34.463 177 65 4 174 348 71 198 4.18e-16 77.8
MS.gene56480.t1 MTR_6g014850 45.098 102 29 2 1 102 1 75 1.87e-13 70.1
MS.gene56480.t1 MTR_6g014820 41.304 92 18 1 1 92 23 78 2.28e-12 64.3
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene56480.t1 AT3G29360 84.167 480 76 0 1 480 1 480 0.0 856
MS.gene56480.t1 AT3G29360 84.167 480 76 0 1 480 1 480 0.0 856
MS.gene56480.t1 AT5G39320 82.845 478 82 0 1 478 1 478 0.0 851
MS.gene56480.t1 AT5G15490 82.708 480 83 0 1 480 1 480 0.0 847
MS.gene56480.t1 AT1G26570 80.457 481 93 1 1 480 1 481 0.0 816
MS.gene56480.t1 AT3G01010 72.393 163 40 2 318 480 1 158 6.24e-83 253

Find 122 sgRNAs with CRISPR-Local

Find 141 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TGAAAGATATTTATGCTAAT+TGG 0.091386 4.4:+30081303 MS.gene56480:CDS
GTTGGTAAGCTCAGGAAAAT+TGG 0.259126 4.4:+30082085 MS.gene56480:CDS
GTACCCTCAGCAAGAAATTC+AGG 0.280180 4.4:-30081115 None:intergenic
TCTAAGAAGATTGCTGTTCT+TGG 0.281241 4.4:+30081701 MS.gene56480:CDS
TTACTGGAAGCAAGTGATTA+AGG 0.289704 4.4:+30081613 MS.gene56480:CDS
CTGTTCCAACCGTTGATTCT+CGG 0.332760 4.4:-30080758 None:intergenic
TTGAATGCTAGTGTTGGTTT+TGG 0.346083 4.4:+30081515 MS.gene56480:CDS
GAAAGATATTTATGCTAATT+GGG 0.354607 4.4:+30081304 MS.gene56480:CDS
TGTTAAATCAGCCGCTTTGC+CGG 0.358742 4.4:-30080939 None:intergenic
AGAGGATTCTGACTCATAAT+AGG 0.360802 4.4:+30081062 MS.gene56480:CDS
ATTGCTTCAGCTGTTCTAAC+AGG 0.364317 4.4:-30081037 None:intergenic
AAGCGGCTGATTTAACATAC+TGG 0.368249 4.4:+30080945 MS.gene56480:CDS
TTCATGTGGTAGCAAATTAC+TGG 0.378490 4.4:+30081597 MS.gene56480:CDS
TGCTTTATCGAAGTTGGACT+TGG 0.380856 4.4:-30081905 None:intergenic
CAAGGGTTTGTTGGGAGATA+AGG 0.381297 4.4:+30081781 MS.gene56480:CDS
GCCACTTGCTTTATCGAAGT+TGG 0.388159 4.4:-30081911 None:intergenic
AAAGCAAGTGGCTGTGGTTT+GGG 0.389789 4.4:+30081922 MS.gene56480:CDS
GTCTGCTGAGCTCTCTAAAC+TGG 0.391926 4.4:+30081361 MS.gene56480:CDS
ACCACGGTTACTGCAATCTT+AGG 0.392452 4.4:-30080722 None:intergenic
GGATTTATTGTTTATTCCAT+TGG 0.392509 4.4:+30082106 MS.gene56480:CDS
ATTTGATGGTAGGAATGTTA+TGG 0.395140 4.4:+30082060 MS.gene56480:CDS
TAAAGCAAGTGGCTGTGGTT+TGG 0.402728 4.4:+30081921 MS.gene56480:CDS
CCACTTCAGTGCTGAAGAAA+AGG 0.417004 4.4:-30080844 None:intergenic
CGATGTGTGCAAGGGTTTGT+TGG 0.421184 4.4:+30081772 MS.gene56480:CDS
AGAGATTCTCTGTGCCAAAA+AGG 0.424500 4.4:-30081393 None:intergenic
TGTGGTTTGGGATGCTTATG+AGG 0.427030 4.4:+30081934 MS.gene56480:CDS
ATCCTTTATGATCTGATCTT+CGG 0.439079 4.4:-30081834 None:intergenic
AAAATTTGTTGCATCGGAGC+TGG 0.445503 4.4:+30080656 MS.gene56480:CDS
AGCCCGGATCGTGTCCTAAT+CGG 0.446831 4.4:+30081239 MS.gene56480:CDS
CAACAACTCGGTTCACAAAC+CGG 0.448174 4.4:-30081655 None:intergenic
GTCTATATTTGCGAGTGTAA+TGG 0.448773 4.4:+30081572 MS.gene56480:CDS
GTGTCTCCCTACCTCCGATT+AGG 0.449487 4.4:-30081253 None:intergenic
TGCAATCTTAGGACACTTCA+AGG 0.467490 4.4:-30080711 None:intergenic
GATCGCCGATGTTTCTAAAT+CGG 0.469822 4.4:+30080985 MS.gene56480:CDS
TGGTGGCTCTTGCTTTCAGA+AGG 0.474973 4.4:+30081535 MS.gene56480:CDS
GGACACGATCCGGGCTGAAA+AGG 0.485556 4.4:-30081232 None:intergenic
ATCGGAACAGACTCGAGGAT+TGG 0.486245 4.4:+30081482 MS.gene56480:CDS
TTATTCCATTGGTAAGCCAC+TGG 0.486901 4.4:+30082117 MS.gene56480:CDS
AAGCCGTGGTGAAGGAGTGC+AGG 0.486980 4.4:+30080813 MS.gene56480:CDS
TTCGATAAAGCAAGTGGCTG+TGG 0.488251 4.4:+30081916 MS.gene56480:CDS
TACCGAAGATCAGATCATAA+AGG 0.492354 4.4:+30081832 MS.gene56480:CDS
GATGTGTGCAAGGGTTTGTT+GGG 0.501277 4.4:+30081773 MS.gene56480:CDS
CCCTTGCACACATCGATAGC+CGG 0.502325 4.4:-30081764 None:intergenic
TACCCTCAGCAAGAAATTCA+GGG 0.503132 4.4:-30081114 None:intergenic
TTCACCACGGCTTCAAGACC+AGG 0.505299 4.4:-30080803 None:intergenic
AGCAACTGGTGCTGATATCT+CGG 0.508415 4.4:+30081445 MS.gene56480:CDS
AAAACTCATGGTCTTGGAGC+CGG 0.509641 4.4:+30080920 MS.gene56480:CDS
TCTTGGTTTCTCTTTCAAGA+AGG 0.511984 4.4:+30081718 MS.gene56480:CDS
TTTATCGAAGTTGGACTTGG+TGG 0.513987 4.4:-30081902 None:intergenic
TCAGAAGGATATAATGAACT+TGG 0.517285 4.4:+30081550 MS.gene56480:CDS
GGTAGGAATGTTATGGATGT+TGG 0.519091 4.4:+30082067 MS.gene56480:CDS
ATGGTGAAAATTTGTTGCAT+CGG 0.519668 4.4:+30080650 MS.gene56480:CDS
TTATGGATGTTGGTAAGCTC+AGG 0.521362 4.4:+30082077 MS.gene56480:CDS
GGAGACACCAGAAGGACAAA+AGG 0.523750 4.4:+30081268 MS.gene56480:CDS
TTGGTAAGCCACTGGATCCA+TGG 0.524811 4.4:+30082125 MS.gene56480:CDS
TTATGCTAATTGGGTCCCTA+AGG 0.524851 4.4:+30081313 MS.gene56480:CDS
AGAAGGACACAGGCGATACA+AGG 0.525027 4.4:+30081735 MS.gene56480:CDS
CTTGGTGGTTGAAGATGAGC+AGG 0.525970 4.4:-30081887 None:intergenic
ACCGGCTATCGATGTGTGCA+AGG 0.530309 4.4:+30081763 MS.gene56480:CDS
TGGTCTTGAAGCCGTGGTGA+AGG 0.539097 4.4:+30080805 MS.gene56480:CDS
CAAGGCAATCACAGCCATTG+TGG 0.541163 4.4:-30080693 None:intergenic
TTCTAAATCGGACAAAATTG+TGG 0.542076 4.4:+30080997 MS.gene56480:CDS
AACCCTGAATTTCTTGCTGA+GGG 0.543013 4.4:+30081112 MS.gene56480:CDS
ATTTCAGCACCGAGAATCAA+CGG 0.543330 4.4:+30080749 MS.gene56480:CDS
TCAAGACCAGGCTCGTAGAT+CGG 0.543878 4.4:-30080791 None:intergenic
TTAATGACTATCAGAAAAGC+CGG 0.546119 4.4:+30081636 MS.gene56480:CDS
TCGGAGGTATCACACTCAAT+CGG 0.548115 4.4:+30081464 MS.gene56480:CDS
GAGCTCAGCAGACCACAGAT+TGG 0.552085 4.4:-30081351 None:intergenic
AAGGCAATCACAGCCATTGT+GGG 0.553236 4.4:-30080692 None:intergenic
TTCTCTTTCAAGAAGGACAC+AGG 0.554715 4.4:+30081725 MS.gene56480:CDS
TCCTTGAGCCATGGATCCAG+TGG 0.560633 4.4:-30082133 None:intergenic
TGGATCCAGTGGCTTACCAA+TGG 0.562663 4.4:-30082122 None:intergenic
CCTCCGATTAGGACACGATC+CGG 0.564937 4.4:-30081242 None:intergenic
TCGTGTCCTAATCGGAGGTA+GGG 0.567068 4.4:+30081247 MS.gene56480:CDS
GCAGGCATGTCCTTGAGCCA+TGG 0.569217 4.4:-30082142 None:intergenic
AGGCGATACAAGGGAAGCAC+CGG 0.573278 4.4:+30081745 MS.gene56480:CDS
TGCATCGGAGCTGGATATGT+TGG 0.574012 4.4:+30080665 MS.gene56480:CDS
CGAGCCTGGTCTTGAAGCCG+TGG 0.575665 4.4:+30080799 MS.gene56480:CDS
TGGTGAAGGAGTGCAGGGGA+AGG 0.576100 4.4:+30080819 MS.gene56480:CDS
CACCTACCGATCTACGAGCC+TGG 0.576813 4.4:+30080785 MS.gene56480:CDS
AGCGGCTGATTTAACATACT+GGG 0.577833 4.4:+30080946 MS.gene56480:CDS
AAACCCTGAATTTCTTGCTG+AGG 0.578960 4.4:+30081111 MS.gene56480:CDS
ATCTGATCTTCGGTAACTTG+AGG 0.579881 4.4:-30081824 None:intergenic
TCAGCACTATGTGAAGCAAC+TGG 0.581495 4.4:+30081431 MS.gene56480:CDS
CAGCACCGAGAATCAACGGT+TGG 0.582970 4.4:+30080753 MS.gene56480:CDS
ACACTAGCATTCAAGAACTT+AGG 0.584911 4.4:-30081506 None:intergenic
AAGTTCTTGAATGCTAGTGT+TGG 0.587122 4.4:+30081509 MS.gene56480:CDS
GTTTGTTGGGAGATAAGGCA+AGG 0.587768 4.4:+30081786 MS.gene56480:CDS
AGAAGCAACCGTGTTGAACA+TGG 0.590789 4.4:-30081681 None:intergenic
GCCACTGGATCCATGGCTCA+AGG 0.597097 4.4:+30082132 MS.gene56480:CDS
TCCAACTTCGATAAAGCAAG+TGG 0.600253 4.4:+30081910 MS.gene56480:CDS
TTTGTCCGATTTAGAAACAT+CGG 0.602624 4.4:-30080990 None:intergenic
ATCGGAGCTGGATATGTTGG+TGG 0.603146 4.4:+30080668 MS.gene56480:CDS
AGCCGTGGTGAAGGAGTGCA+GGG 0.608786 4.4:+30080814 MS.gene56480:CDS
AAATCATTTGCACCAATCTG+TGG 0.612903 4.4:+30081339 MS.gene56480:CDS
ATCGTGTCCTAATCGGAGGT+AGG 0.615001 4.4:+30081246 MS.gene56480:CDS
GCTCAAGGACATGCCTGCAG+TGG 0.625363 4.4:+30082147 MS.gene56480:CDS
TCCCCTGCACTCCTTCACCA+CGG 0.628514 4.4:-30080816 None:intergenic
TGAACATGGACGCAACAACT+CGG 0.630690 4.4:-30081667 None:intergenic
CCGGCTATCGATGTGTGCAA+GGG 0.631110 4.4:+30081764 MS.gene56480:CDS
CTCCGATTAGGACACGATCC+GGG 0.633554 4.4:-30081241 None:intergenic
GACCAGGCTCGTAGATCGGT+AGG 0.634392 4.4:-30080787 None:intergenic
TGTTGCGTCCATGTTCAACA+CGG 0.638044 4.4:+30081673 MS.gene56480:CDS
GAAGGACACAGGCGATACAA+GGG 0.639231 4.4:+30081736 MS.gene56480:CDS
GAACAGCTGAAGCAATTGAG+AGG 0.641777 4.4:+30081044 MS.gene56480:CDS
GGAGGTAGGGAGACACCAGA+AGG 0.655737 4.4:+30081260 MS.gene56480:CDS
CCGGATCGTGTCCTAATCGG+AGG 0.664959 4.4:+30081242 MS.gene56480:CDS
TCCTAAGATTGCAGTAACCG+TGG 0.667449 4.4:+30080721 MS.gene56480:CDS
CGAGTGTAATGGTCTTCATG+TGG 0.667527 4.4:+30081583 MS.gene56480:CDS
ATATGTTGGTGGTCCCACAA+TGG 0.680415 4.4:+30080679 MS.gene56480:CDS
TGGTCTTGGAGCCGGCAAAG+CGG 0.691470 4.4:+30080928 MS.gene56480:CDS
ACTCAATCGGAACAGACTCG+AGG 0.702881 4.4:+30081477 MS.gene56480:CDS
AACTGGTGCTGATATCTCGG+AGG 0.704506 4.4:+30081448 MS.gene56480:CDS
CATACTGGGAGAGTTCCGCT+CGG 0.706063 4.4:+30080960 MS.gene56480:CDS
GTGTTTGCATTATGACTGAG+TGG 0.706540 4.4:+30081975 MS.gene56480:CDS
TGTTTGCATTATGACTGAGT+GGG 0.707780 4.4:+30081976 MS.gene56480:CDS
CGGTGCTGAAATATCGACCA+CGG 0.712301 4.4:-30080738 None:intergenic
CAGGCTCGTAGATCGGTAGG+TGG 0.714440 4.4:-30080784 None:intergenic
GCCGTGGTGAAGGAGTGCAG+GGG 0.744462 4.4:+30080815 MS.gene56480:CDS
GGTTTGGGATGCTTATGAGG+CGG 0.748746 4.4:+30081937 MS.gene56480:CDS
AACATCGGCGATCATCCGAG+CGG 0.754134 4.4:-30080975 None:intergenic
GAGGCGGTGAAAGATGCACA+CGG 0.755151 4.4:+30081953 MS.gene56480:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! GAAAGATATTTATGCTAATT+GGG + chr4.4:30081304-30081323 MS.gene56480:CDS 20.0%
!! TGAAAGATATTTATGCTAAT+TGG + chr4.4:30081303-30081322 MS.gene56480:CDS 20.0%
!!! AATTTTATCTGTAATAGGTA+CGG + chr4.4:30081199-30081218 MS.gene56480:intron 20.0%
!!! GCATATTGTCATAAATTTTT+TGG - chr4.4:30082024-30082043 None:intergenic 20.0%
! AATTCCTGAAATAGTTAGTT+AGG + chr4.4:30081138-30081157 MS.gene56480:intron 25.0%
! ATAACCTAACTAACTATTTC+AGG - chr4.4:30081145-30081164 None:intergenic 25.0%
! GGATTTATTGTTTATTCCAT+TGG + chr4.4:30082106-30082125 MS.gene56480:CDS 25.0%
! GTACCTATTACAGATAAAAT+TGG - chr4.4:30081200-30081219 None:intergenic 25.0%
!! GTACCAATTTTATCTGTAAT+AGG + chr4.4:30081194-30081213 MS.gene56480:intron 25.0%
ATCCTTTATGATCTGATCTT+CGG - chr4.4:30081837-30081856 None:intergenic 30.0%
CTACCATCAAATAAAAATGC+TGG - chr4.4:30082052-30082071 None:intergenic 30.0%
TCAGAAGGATATAATGAACT+TGG + chr4.4:30081550-30081569 MS.gene56480:CDS 30.0%
TTAATGACTATCAGAAAAGC+CGG + chr4.4:30081636-30081655 MS.gene56480:CDS 30.0%
TTCTAAATCGGACAAAATTG+TGG + chr4.4:30080997-30081016 MS.gene56480:CDS 30.0%
TTTGTCCGATTTAGAAACAT+CGG - chr4.4:30080993-30081012 None:intergenic 30.0%
! ATTTGATGGTAGGAATGTTA+TGG + chr4.4:30082060-30082079 MS.gene56480:CDS 30.0%
! TGCAAATGATTTTCTCCTTA+GGG - chr4.4:30081331-30081350 None:intergenic 30.0%
!! AAGCCAGCATTTTTATTTGA+TGG + chr4.4:30082046-30082065 MS.gene56480:CDS 30.0%
!! ATGGTGAAAATTTGTTGCAT+CGG + chr4.4:30080650-30080669 MS.gene56480:CDS 30.0%
!! CAGCATTTTTATTTGATGGT+AGG + chr4.4:30082050-30082069 MS.gene56480:CDS 30.0%
AAATCATTTGCACCAATCTG+TGG + chr4.4:30081339-30081358 MS.gene56480:CDS 35.0%
AACACACCAACAAAAACTCA+TGG + chr4.4:30080908-30080927 MS.gene56480:CDS 35.0%
AAGTTCTTGAATGCTAGTGT+TGG + chr4.4:30081509-30081528 MS.gene56480:CDS 35.0%
ACACTAGCATTCAAGAACTT+AGG - chr4.4:30081509-30081528 None:intergenic 35.0%
AGAGGATTCTGACTCATAAT+AGG + chr4.4:30081062-30081081 MS.gene56480:CDS 35.0%
GTCTATATTTGCGAGTGTAA+TGG + chr4.4:30081572-30081591 MS.gene56480:CDS 35.0%
TACCGAAGATCAGATCATAA+AGG + chr4.4:30081832-30081851 MS.gene56480:CDS 35.0%
TCTTGGTTTCTCTTTCAAGA+AGG + chr4.4:30081718-30081737 MS.gene56480:CDS 35.0%
TGTTTGCATTATGACTGAGT+GGG + chr4.4:30081976-30081995 MS.gene56480:CDS 35.0%
TTACTGGAAGCAAGTGATTA+AGG + chr4.4:30081613-30081632 MS.gene56480:CDS 35.0%
TTCATGTGGTAGCAAATTAC+TGG + chr4.4:30081597-30081616 MS.gene56480:CDS 35.0%
! GTGCAAATGATTTTCTCCTT+AGG - chr4.4:30081332-30081351 None:intergenic 35.0%
! TCTAAGAAGATTGCTGTTCT+TGG + chr4.4:30081701-30081720 MS.gene56480:CDS 35.0%
!!! TTGAATGCTAGTGTTGGTTT+TGG + chr4.4:30081515-30081534 MS.gene56480:CDS 35.0%
AAACCCTGAATTTCTTGCTG+AGG + chr4.4:30081111-30081130 MS.gene56480:CDS 40.0%
AACCCTGAATTTCTTGCTGA+GGG + chr4.4:30081112-30081131 MS.gene56480:CDS 40.0%
AGAGATTCTCTGTGCCAAAA+AGG - chr4.4:30081396-30081415 None:intergenic 40.0%
ATCTGATCTTCGGTAACTTG+AGG - chr4.4:30081827-30081846 None:intergenic 40.0%
ATTGCTTCAGCTGTTCTAAC+AGG - chr4.4:30081040-30081059 None:intergenic 40.0%
CCAACAAAAACTCATGGTCT+TGG + chr4.4:30080914-30080933 MS.gene56480:CDS 40.0%
GGTAGGAATGTTATGGATGT+TGG + chr4.4:30082067-30082086 MS.gene56480:CDS 40.0%
GTGTTTGCATTATGACTGAG+TGG + chr4.4:30081975-30081994 MS.gene56480:CDS 40.0%
TACCCTCAGCAAGAAATTCA+GGG - chr4.4:30081117-30081136 None:intergenic 40.0%
TCCAACTTCGATAAAGCAAG+TGG + chr4.4:30081910-30081929 MS.gene56480:CDS 40.0%
TGCAATCTTAGGACACTTCA+AGG - chr4.4:30080714-30080733 None:intergenic 40.0%
TGCTTTATCGAAGTTGGACT+TGG - chr4.4:30081908-30081927 None:intergenic 40.0%
TTATGCTAATTGGGTCCCTA+AGG + chr4.4:30081313-30081332 MS.gene56480:CDS 40.0%
TTATTCCATTGGTAAGCCAC+TGG + chr4.4:30082117-30082136 MS.gene56480:CDS 40.0%
TTCTCTTTCAAGAAGGACAC+AGG + chr4.4:30081725-30081744 MS.gene56480:CDS 40.0%
TTTATCGAAGTTGGACTTGG+TGG - chr4.4:30081905-30081924 None:intergenic 40.0%
! AAGCGGCTGATTTAACATAC+TGG + chr4.4:30080945-30080964 MS.gene56480:CDS 40.0%
! AATCAAAGACCTTTTCAGCC+CGG + chr4.4:30081223-30081242 MS.gene56480:CDS 40.0%
! AGCGGCTGATTTAACATACT+GGG + chr4.4:30080946-30080965 MS.gene56480:CDS 40.0%
! ATTTCAGCACCGAGAATCAA+CGG + chr4.4:30080749-30080768 MS.gene56480:CDS 40.0%
! CCAAGACCATGAGTTTTTGT+TGG - chr4.4:30080917-30080936 None:intergenic 40.0%
! GATCGCCGATGTTTCTAAAT+CGG + chr4.4:30080985-30081004 MS.gene56480:CDS 40.0%
! GTTGGTAAGCTCAGGAAAAT+TGG + chr4.4:30082085-30082104 MS.gene56480:CDS 40.0%
! TCTATAGCCTTTTGTCCTTC+TGG - chr4.4:30081278-30081297 None:intergenic 40.0%
! TTATGGATGTTGGTAAGCTC+AGG + chr4.4:30082077-30082096 MS.gene56480:CDS 40.0%
!! AAAATTTGTTGCATCGGAGC+TGG + chr4.4:30080656-30080675 MS.gene56480:CDS 40.0%
!!! AATGCTAGTGTTGGTTTTGG+TGG + chr4.4:30081518-30081537 MS.gene56480:CDS 40.0%
AAAGCAAGTGGCTGTGGTTT+GGG + chr4.4:30081922-30081941 MS.gene56480:CDS 45.0%
AAGGCAATCACAGCCATTGT+GGG - chr4.4:30080695-30080714 None:intergenic 45.0%
ACCACGGTTACTGCAATCTT+AGG - chr4.4:30080725-30080744 None:intergenic 45.0%
AGAAGCAACCGTGTTGAACA+TGG - chr4.4:30081684-30081703 None:intergenic 45.0%
CAACAACTCGGTTCACAAAC+CGG - chr4.4:30081658-30081677 None:intergenic 45.0%
CGAGTGTAATGGTCTTCATG+TGG + chr4.4:30081583-30081602 MS.gene56480:CDS 45.0%
GAACAGCTGAAGCAATTGAG+AGG + chr4.4:30081044-30081063 MS.gene56480:CDS 45.0%
GCCACTTGCTTTATCGAAGT+TGG - chr4.4:30081914-30081933 None:intergenic 45.0%
GTACCCTCAGCAAGAAATTC+AGG - chr4.4:30081118-30081137 None:intergenic 45.0%
TAAAGCAAGTGGCTGTGGTT+TGG + chr4.4:30081921-30081940 MS.gene56480:CDS 45.0%
TCAGCACTATGTGAAGCAAC+TGG + chr4.4:30081431-30081450 MS.gene56480:CDS 45.0%
TCCTAAGATTGCAGTAACCG+TGG + chr4.4:30080721-30080740 MS.gene56480:CDS 45.0%
TGAACATGGACGCAACAACT+CGG - chr4.4:30081670-30081689 None:intergenic 45.0%
TGTTGCGTCCATGTTCAACA+CGG + chr4.4:30081673-30081692 MS.gene56480:CDS 45.0%
TTCGATAAAGCAAGTGGCTG+TGG + chr4.4:30081916-30081935 MS.gene56480:CDS 45.0%
! AAAACTCATGGTCTTGGAGC+CGG + chr4.4:30080920-30080939 MS.gene56480:CDS 45.0%
! CCACTTCAGTGCTGAAGAAA+AGG - chr4.4:30080847-30080866 None:intergenic 45.0%
! GATGTGTGCAAGGGTTTGTT+GGG + chr4.4:30081773-30081792 MS.gene56480:CDS 45.0%
! GTTTGTTGGGAGATAAGGCA+AGG + chr4.4:30081786-30081805 MS.gene56480:CDS 45.0%
! TGTGGTTTGGGATGCTTATG+AGG + chr4.4:30081934-30081953 MS.gene56480:CDS 45.0%
! TGTTAAATCAGCCGCTTTGC+CGG - chr4.4:30080942-30080961 None:intergenic 45.0%
!! AGCAACTGGTGCTGATATCT+CGG + chr4.4:30081445-30081464 MS.gene56480:CDS 45.0%
!! ATATGTTGGTGGTCCCACAA+TGG + chr4.4:30080679-30080698 MS.gene56480:CDS 45.0%
!! CAAGGGTTTGTTGGGAGATA+AGG + chr4.4:30081781-30081800 MS.gene56480:CDS 45.0%
!! CCTTTTCTTCAGCACTGAAG+TGG + chr4.4:30080844-30080863 MS.gene56480:CDS 45.0%
!! CTGTTCCAACCGTTGATTCT+CGG - chr4.4:30080761-30080780 None:intergenic 45.0%
!! TCGGAGGTATCACACTCAAT+CGG + chr4.4:30081464-30081483 MS.gene56480:CDS 45.0%
ACTCAATCGGAACAGACTCG+AGG + chr4.4:30081477-30081496 MS.gene56480:CDS 50.0%
AGAAGGACACAGGCGATACA+AGG + chr4.4:30081735-30081754 MS.gene56480:CDS 50.0%
ATCGGAACAGACTCGAGGAT+TGG + chr4.4:30081482-30081501 MS.gene56480:CDS 50.0%
ATCGGAGCTGGATATGTTGG+TGG + chr4.4:30080668-30080687 MS.gene56480:CDS 50.0%
ATCGTGTCCTAATCGGAGGT+AGG + chr4.4:30081246-30081265 MS.gene56480:CDS 50.0%
CAAGGCAATCACAGCCATTG+TGG - chr4.4:30080696-30080715 None:intergenic 50.0%
CGATGTGTGCAAGGGTTTGT+TGG + chr4.4:30081772-30081791 MS.gene56480:CDS 50.0%
GAAGGACACAGGCGATACAA+GGG + chr4.4:30081736-30081755 MS.gene56480:CDS 50.0%
GGAGACACCAGAAGGACAAA+AGG + chr4.4:30081268-30081287 MS.gene56480:CDS 50.0%
GTCTGCTGAGCTCTCTAAAC+TGG + chr4.4:30081361-30081380 MS.gene56480:CDS 50.0%
TCAAGACCAGGCTCGTAGAT+CGG - chr4.4:30080794-30080813 None:intergenic 50.0%
TCGTGTCCTAATCGGAGGTA+GGG + chr4.4:30081247-30081266 MS.gene56480:CDS 50.0%
TGCATCGGAGCTGGATATGT+TGG + chr4.4:30080665-30080684 MS.gene56480:CDS 50.0%
TGGATCCAGTGGCTTACCAA+TGG - chr4.4:30082125-30082144 None:intergenic 50.0%
TGGTGGCTCTTGCTTTCAGA+AGG + chr4.4:30081535-30081554 MS.gene56480:CDS 50.0%
TTGGTAAGCCACTGGATCCA+TGG + chr4.4:30082125-30082144 MS.gene56480:CDS 50.0%
! ACTGGCTGCTAACGCCTTTT+TGG + chr4.4:30081379-30081398 MS.gene56480:CDS 50.0%
! CGGTGCTGAAATATCGACCA+CGG - chr4.4:30080741-30080760 None:intergenic 50.0%
! CTTGGTGGTTGAAGATGAGC+AGG - chr4.4:30081890-30081909 None:intergenic 50.0%
! GGTTTGGGATGCTTATGAGG+CGG + chr4.4:30081937-30081956 MS.gene56480:CDS 50.0%
!! AACTGGTGCTGATATCTCGG+AGG + chr4.4:30081448-30081467 MS.gene56480:CDS 50.0%
AACATCGGCGATCATCCGAG+CGG - chr4.4:30080978-30080997 None:intergenic 55.0%
ACCGGCTATCGATGTGTGCA+AGG + chr4.4:30081763-30081782 MS.gene56480:CDS 55.0%
AGCCCGGATCGTGTCCTAAT+CGG + chr4.4:30081239-30081258 MS.gene56480:CDS 55.0%
CATACTGGGAGAGTTCCGCT+CGG + chr4.4:30080960-30080979 MS.gene56480:CDS 55.0%
CCCTTGCACACATCGATAGC+CGG - chr4.4:30081767-30081786 None:intergenic 55.0%
CCGGCTATCGATGTGTGCAA+GGG + chr4.4:30081764-30081783 MS.gene56480:CDS 55.0%
CCTCCGATTAGGACACGATC+CGG - chr4.4:30081245-30081264 None:intergenic 55.0%
CTCCGATTAGGACACGATCC+GGG - chr4.4:30081244-30081263 None:intergenic 55.0%
GAGCTCAGCAGACCACAGAT+TGG - chr4.4:30081354-30081373 None:intergenic 55.0%
GAGGCGGTGAAAGATGCACA+CGG + chr4.4:30081953-30081972 MS.gene56480:CDS 55.0%
GTGTCTCCCTACCTCCGATT+AGG - chr4.4:30081256-30081275 None:intergenic 55.0%
TCCTTGAGCCATGGATCCAG+TGG - chr4.4:30082136-30082155 None:intergenic 55.0%
TTCACCACGGCTTCAAGACC+AGG - chr4.4:30080806-30080825 None:intergenic 55.0%
! AGGCGATACAAGGGAAGCAC+CGG + chr4.4:30081745-30081764 MS.gene56480:CDS 55.0%
! CAGCACCGAGAATCAACGGT+TGG + chr4.4:30080753-30080772 MS.gene56480:CDS 55.0%
! TGGTCTTGAAGCCGTGGTGA+AGG + chr4.4:30080805-30080824 MS.gene56480:CDS 55.0%
CACCTACCGATCTACGAGCC+TGG + chr4.4:30080785-30080804 MS.gene56480:CDS 60.0%
CAGGCTCGTAGATCGGTAGG+TGG - chr4.4:30080787-30080806 None:intergenic 60.0%
CCGGATCGTGTCCTAATCGG+AGG + chr4.4:30081242-30081261 MS.gene56480:CDS 60.0%
GACCAGGCTCGTAGATCGGT+AGG - chr4.4:30080790-30080809 None:intergenic 60.0%
GCAGGCATGTCCTTGAGCCA+TGG - chr4.4:30082145-30082164 None:intergenic 60.0%
GCCACTGGATCCATGGCTCA+AGG + chr4.4:30082132-30082151 MS.gene56480:CDS 60.0%
GCTCAAGGACATGCCTGCAG+TGG + chr4.4:30082147-30082166 MS.gene56480:CDS 60.0%
GGACACGATCCGGGCTGAAA+AGG - chr4.4:30081235-30081254 None:intergenic 60.0%
GGAGGTAGGGAGACACCAGA+AGG + chr4.4:30081260-30081279 MS.gene56480:CDS 60.0%
TCCCCTGCACTCCTTCACCA+CGG - chr4.4:30080819-30080838 None:intergenic 60.0%
! AAGCCGTGGTGAAGGAGTGC+AGG + chr4.4:30080813-30080832 MS.gene56480:CDS 60.0%
! AGCCGTGGTGAAGGAGTGCA+GGG + chr4.4:30080814-30080833 MS.gene56480:CDS 60.0%
! TGGTCTTGGAGCCGGCAAAG+CGG + chr4.4:30080928-30080947 MS.gene56480:CDS 60.0%
! TGGTGAAGGAGTGCAGGGGA+AGG + chr4.4:30080819-30080838 MS.gene56480:CDS 60.0%
! GCCGTGGTGAAGGAGTGCAG+GGG + chr4.4:30080815-30080834 MS.gene56480:CDS 65.0%
!! CGAGCCTGGTCTTGAAGCCG+TGG + chr4.4:30080799-30080818 MS.gene56480:CDS 65.0%


Chromosome Type Strat End Strand Name
chr4.4 gene 30080650 30082174 30080650 ID=MS.gene56480
chr4.4 mRNA 30080650 30082174 30080650 ID=MS.gene56480.t1;Parent=MS.gene56480
chr4.4 exon 30080650 30081133 30080650 ID=MS.gene56480.t1.exon1;Parent=MS.gene56480.t1
chr4.4 CDS 30080650 30081133 30080650 ID=cds.MS.gene56480.t1;Parent=MS.gene56480.t1
chr4.4 exon 30081216 30082174 30081216 ID=MS.gene56480.t1.exon2;Parent=MS.gene56480.t1
chr4.4 CDS 30081216 30082174 30081216 ID=cds.MS.gene56480.t1;Parent=MS.gene56480.t1
Gene Sequence

>MS.gene56480

ATGGTGAAAATTTGTTGCATCGGAGCTGGATATGTTGGTGGTCCCACAATGGCTGTGATTGCCTTGAAGTGTCCTAAGATTGCAGTAACCGTGGTCGATATTTCAGCACCGAGAATCAACGGTTGGAACAGCGACCACCTACCGATCTACGAGCCTGGTCTTGAAGCCGTGGTGAAGGAGTGCAGGGGAAGGAACCTTTTCTTCAGCACTGAAGTGGAGAAACATGTTGCAGAAGCTGATATAGTCTTTGTATCTGTGAACACACCAACAAAAACTCATGGTCTTGGAGCCGGCAAAGCGGCTGATTTAACATACTGGGAGAGTTCCGCTCGGATGATCGCCGATGTTTCTAAATCGGACAAAATTGTGGTTGAAAAATCAACTGTACCTGTTAGAACAGCTGAAGCAATTGAGAGGATTCTGACTCATAATAGGAAAAAAATCAATTTCACTATTTTGTCAAACCCTGAATTTCTTGCTGAGGGTACAATTCCTGAAATAGTTAGTTAGGTTATTTAATTTTGTTATCTTCGTAACTAATTATGTACCAATTTTATCTGTAATAGGTACGGCAATCAAAGACCTTTTCAGCCCGGATCGTGTCCTAATCGGAGGTAGGGAGACACCAGAAGGACAAAAGGCTATAGAAACATTGAAAGATATTTATGCTAATTGGGTCCCTAAGGAGAAAATCATTTGCACCAATCTGTGGTCTGCTGAGCTCTCTAAACTGGCTGCTAACGCCTTTTTGGCACAGAGAATCTCTTCTGTGAATGCTATGTCAGCACTATGTGAAGCAACTGGTGCTGATATCTCGGAGGTATCACACTCAATCGGAACAGACTCGAGGATTGGACCTAAGTTCTTGAATGCTAGTGTTGGTTTTGGTGGCTCTTGCTTTCAGAAGGATATAATGAACTTGGTCTATATTTGCGAGTGTAATGGTCTTCATGTGGTAGCAAATTACTGGAAGCAAGTGATTAAGGTTAATGACTATCAGAAAAGCCGGTTTGTGAACCGAGTTGTTGCGTCCATGTTCAACACGGTTGCTTCTAAGAAGATTGCTGTTCTTGGTTTCTCTTTCAAGAAGGACACAGGCGATACAAGGGAAGCACCGGCTATCGATGTGTGCAAGGGTTTGTTGGGAGATAAGGCAAGGTTGAGCATATACGATCCTCAAGTTACCGAAGATCAGATCATAAAGGATCTGTCGATGAAGAAATTCGATAAAGAACAGCCTGCTCATCTTCAACCACCAAGTCCAACTTCGATAAAGCAAGTGGCTGTGGTTTGGGATGCTTATGAGGCGGTGAAAGATGCACACGGTGTTTGCATTATGACTGAGTGGGACGAGTTTAAGAAACTCGATTACCAAAAAATTTATGACAATATGCAGAAGCCAGCATTTTTATTTGATGGTAGGAATGTTATGGATGTTGGTAAGCTCAGGAAAATTGGATTTATTGTTTATTCCATTGGTAAGCCACTGGATCCATGGCTCAAGGACATGCCTGCAGTGGCATAA
Protein sequence

>MS.gene56480.t1

MVKICCIGAGYVGGPTMAVIALKCPKIAVTVVDISAPRINGWNSDHLPIYEPGLEAVVKECRGRNLFFSTEVEKHVAEADIVFVSVNTPTKTHGLGAGKAADLTYWESSARMIADVSKSDKIVVEKSTVPVRTAEAIERILTHNRKKINFTILSNPEFLAEGTAIKDLFSPDRVLIGGRETPEGQKAIETLKDIYANWVPKEKIICTNLWSAELSKLAANAFLAQRISSVNAMSALCEATGADISEVSHSIGTDSRIGPKFLNASVGFGGSCFQKDIMNLVYICECNGLHVVANYWKQVIKVNDYQKSRFVNRVVASMFNTVASKKIAVLGFSFKKDTGDTREAPAIDVCKGLLGDKARLSIYDPQVTEDQIIKDLSMKKFDKEQPAHLQPPSPTSIKQVAVVWDAYEAVKDAHGVCIMTEWDEFKKLDYQKIYDNMQKPAFLFDGRNVMDVGKLRKIGFIVYSIGKPLDPWLKDMPAVA