AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene56532

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene56532.t1 MTR_4g081950 99.597 248 1 0 1 248 37 284 0.0 506
MS.gene56532.t1 MTR_5g041700 86.235 247 34 0 1 247 1 247 1.73e-163 452
MS.gene56532.t1 MTR_3g094040 80.165 242 48 0 6 247 12 253 7.15e-146 408
MS.gene56532.t1 MTR_3g094060 80.165 242 48 0 6 247 12 253 1.35e-145 407
MS.gene56532.t1 MTR_3g435430 82.051 234 42 0 14 247 14 247 2.19e-144 404
MS.gene56532.t1 MTR_5g075320 76.829 246 56 1 3 247 6 251 2.57e-139 391
MS.gene56532.t1 MTR_3g466790 77.328 247 56 0 1 247 8 254 1.63e-136 384
MS.gene56532.t1 MTR_3g466760 76.613 248 56 2 1 247 14 260 1.24e-134 380
MS.gene56532.t1 MTR_7g011060 74.297 249 53 3 1 247 1 240 1.95e-132 374
MS.gene56532.t1 MTR_5g079950 70.732 246 69 2 1 244 10 254 9.34e-128 362
MS.gene56532.t1 MTR_6g012520 75.299 251 51 3 1 248 1 243 6.00e-123 349
MS.gene56532.t1 MTR_2g097030 66.667 255 74 2 1 244 1 255 7.40e-123 350
MS.gene56532.t1 MTR_7g061720 67.984 253 72 3 1 244 1 253 7.45e-123 350
MS.gene56532.t1 MTR_1g026020 64.315 241 82 3 6 243 11 250 1.12e-114 329
MS.gene56532.t1 MTR_4g063975 66.000 250 78 3 1 244 5 253 5.46e-114 327
MS.gene56532.t1 MTR_1g085460 64.502 231 78 3 16 242 21 251 4.22e-107 310
MS.gene56532.t1 MTR_7g111010 60.816 245 91 4 3 242 4 248 7.77e-104 301
MS.gene56532.t1 MTR_7g110990 65.315 222 73 3 25 242 12 233 3.64e-103 299
MS.gene56532.t1 MTR_4g124340 57.778 225 90 3 23 244 59 281 6.58e-97 285
MS.gene56532.t1 MTR_7g013120 59.556 225 86 3 25 246 29 251 9.44e-95 278
MS.gene56532.t1 MTR_7g013150 59.091 220 86 2 25 242 30 247 6.67e-94 276
MS.gene56532.t1 MTR_7g013140 58.108 222 89 2 25 244 28 247 2.17e-91 270
MS.gene56532.t1 MTR_8g058990 57.399 223 91 2 25 245 30 250 3.02e-91 270
MS.gene56532.t1 MTR_5g076950 56.250 224 97 1 25 247 41 264 3.38e-90 267
MS.gene56532.t1 MTR_8g464350 56.364 220 92 2 25 242 31 248 5.00e-88 261
MS.gene56532.t1 MTR_5g011210 55.405 222 95 3 25 243 37 257 1.32e-86 258
MS.gene56532.t1 MTR_0040s0140 50.000 250 118 3 1 247 11 256 2.26e-86 257
MS.gene56532.t1 MTR_2g090255 50.000 246 119 2 3 246 8 251 1.93e-84 252
MS.gene56532.t1 MTR_4g102450 55.605 223 93 4 25 243 36 256 2.74e-84 252
MS.gene56532.t1 MTR_3g064510 52.814 231 100 5 25 246 53 283 2.80e-78 238
MS.gene56532.t1 MTR_0008s0120 43.439 221 69 3 25 245 32 196 2.77e-52 168
MS.gene56532.t1 MTR_6g012500 77.228 101 23 0 148 248 181 281 1.41e-51 169
MS.gene56532.t1 MTR_2g090270 30.769 234 132 9 25 243 35 253 1.10e-26 104
MS.gene56532.t1 MTR_2g090265 31.197 234 131 10 25 243 33 251 1.35e-25 101
MS.gene56532.t1 MTR_0118s0070 32.314 229 127 10 30 242 53 269 2.18e-24 98.6
MS.gene56532.t1 MTR_0122s0020 31.602 231 134 9 25 243 41 259 4.90e-24 97.4
MS.gene56532.t1 MTR_4g028640 29.218 243 139 11 16 242 33 258 1.60e-20 88.2
MS.gene56532.t1 MTR_0809s0010 29.536 237 129 12 25 242 47 264 3.46e-20 87.0
MS.gene56532.t1 MTR_4g099370 27.347 245 161 9 6 242 9 244 5.27e-18 80.9
MS.gene56532.t1 MTR_5g013440 26.446 242 163 8 8 242 12 245 1.55e-16 77.0
MS.gene56532.t1 MTR_1g085365 60.784 51 20 0 192 242 4 54 2.00e-15 68.9
MS.gene56532.t1 MTR_4g075790 28.700 223 126 8 36 242 32 237 2.56e-15 73.6
MS.gene56532.t1 MTR_4g098930 29.461 241 135 12 25 247 29 252 3.37e-15 73.2
MS.gene56532.t1 MTR_0122s0020 31.792 173 95 8 25 186 41 201 5.86e-14 68.9
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene56532.t1 AT1G69530 81.928 249 43 2 1 247 1 249 1.53e-150 419
MS.gene56532.t1 AT1G69530 81.928 249 43 2 1 247 1 249 1.53e-150 419
MS.gene56532.t1 AT2G03090 78.947 247 52 0 1 247 6 252 1.78e-149 417
MS.gene56532.t1 AT1G69530 82.041 245 42 2 1 243 1 245 1.88e-148 415
MS.gene56532.t1 AT1G26770 83.471 242 40 0 6 247 7 248 3.57e-141 396
MS.gene56532.t1 AT1G26770 83.471 242 40 0 6 247 17 258 4.83e-141 396
MS.gene56532.t1 AT5G56320 72.908 251 64 1 1 247 1 251 6.65e-136 383
MS.gene56532.t1 AT5G56320 72.908 251 64 1 1 247 1 251 6.65e-136 383
MS.gene56532.t1 AT5G56320 72.908 251 64 1 1 247 1 251 2.64e-135 383
MS.gene56532.t1 AT2G40610 74.074 243 62 1 6 247 11 253 3.91e-135 381
MS.gene56532.t1 AT2G39700 69.444 252 69 2 1 244 1 252 3.01e-127 361
MS.gene56532.t1 AT5G05290 69.231 247 73 2 4 247 9 255 2.03e-125 356
MS.gene56532.t1 AT1G69530 71.486 249 39 3 1 247 1 219 5.92e-125 353
MS.gene56532.t1 AT5G02260 68.145 248 75 2 1 244 6 253 8.96e-125 355
MS.gene56532.t1 AT1G69530 72.917 240 53 4 1 238 1 230 1.04e-124 354
MS.gene56532.t1 AT3G55500 73.333 225 58 1 22 244 31 255 3.20e-124 353
MS.gene56532.t1 AT2G37640 70.124 241 69 2 6 244 18 257 5.26e-124 353
MS.gene56532.t1 AT3G29030 74.107 224 49 2 25 247 38 253 1.02e-119 342
MS.gene56532.t1 AT2G28950 67.063 252 75 2 1 244 1 252 1.05e-117 337
MS.gene56532.t1 AT1G20190 71.300 223 61 2 24 243 25 247 1.96e-114 328
MS.gene56532.t1 AT2G37640 71.707 205 56 1 42 244 28 232 9.26e-110 316
MS.gene56532.t1 AT4G01630 62.241 241 87 3 6 242 9 249 7.28e-107 309
MS.gene56532.t1 AT5G39280 56.067 239 100 2 9 244 35 271 4.34e-98 288
MS.gene56532.t1 AT5G39300 58.636 220 89 1 25 244 55 272 2.66e-97 286
MS.gene56532.t1 AT5G39270 58.182 220 90 1 25 244 58 275 1.90e-93 276
MS.gene56532.t1 AT5G39290 57.273 220 92 1 25 244 58 275 9.79e-93 274
MS.gene56532.t1 AT1G20190 68.449 187 56 2 60 243 2 188 2.88e-91 267
MS.gene56532.t1 AT5G39310 56.757 222 93 2 24 244 89 308 4.37e-89 266
MS.gene56532.t1 AT1G12560 53.527 241 109 3 4 242 16 255 1.60e-86 258
MS.gene56532.t1 AT1G62980 52.893 242 108 5 6 242 10 250 1.19e-85 255
MS.gene56532.t1 AT3G03220 52.679 224 105 1 25 247 40 263 3.84e-84 252
MS.gene56532.t1 AT3G15370 52.675 243 105 6 6 243 8 245 3.89e-82 246
MS.gene56532.t1 AT5G39260 53.846 221 94 2 25 244 45 258 6.07e-79 239
MS.gene56532.t1 AT4G38210 50.661 227 108 2 25 247 28 254 2.74e-78 237
MS.gene56532.t1 AT3G15370 46.886 273 105 7 6 243 8 275 1.47e-75 231
MS.gene56532.t1 AT4G28250 33.190 232 130 9 25 243 36 255 8.27e-28 107
MS.gene56532.t1 AT2G20750 33.047 233 132 9 25 243 40 262 4.81e-26 102
MS.gene56532.t1 AT4G28250 32.468 231 127 9 25 243 36 249 1.98e-24 98.2
MS.gene56532.t1 AT1G65680 30.901 233 134 10 25 242 47 267 7.89e-23 94.4
MS.gene56532.t1 AT1G65681 29.954 217 127 9 39 242 12 216 3.23e-19 83.6
MS.gene56532.t1 AT2G45110 28.750 240 132 9 24 242 32 253 5.11e-19 84.0
MS.gene56532.t1 AT4G17030 26.820 261 162 9 2 248 4 249 6.73e-18 80.5
MS.gene56532.t1 AT4G28250 33.143 175 90 7 25 189 36 193 8.47e-17 77.0
MS.gene56532.t1 AT2G20750 31.214 173 99 6 25 186 40 203 1.95e-16 76.3
MS.gene56532.t1 AT3G60570 25.000 232 146 8 25 242 34 251 2.03e-16 76.6
MS.gene56532.t1 AT4G28250 33.140 172 88 7 25 186 36 190 4.29e-16 74.7
MS.gene56532.t1 AT3G45970 26.147 218 138 10 43 247 14 221 6.00e-14 69.7
MS.gene56532.t1 AT3G45960 29.577 213 126 10 48 247 46 247 1.45e-13 68.9
MS.gene56532.t1 AT4G38400 27.830 212 131 10 48 247 47 248 2.11e-12 65.5
MS.gene56532.t1 AT3G60570 23.832 214 135 8 43 242 4 203 2.88e-12 64.7
MS.gene56532.t1 AT3G45970 25.352 213 136 10 48 247 46 248 2.26e-11 62.8

Find 67 sgRNAs with CRISPR-Local

Find 89 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TTCTCCTCTGTTTATGCTTA+TGG 0.146828 4.4:+30543940 MS.gene56532:CDS
AATGCACATGCTACCTTCTA+TGG 0.227219 4.4:+30543976 MS.gene56532:CDS
GCTCCAGCTGGATGGTCTTT+TGG 0.250334 4.4:+30544819 MS.gene56532:CDS
CACCAACCACCTTGATTGTT+TGG 0.269349 4.4:-30544358 None:intergenic
TCCAGCTGGAGCAACATTAT+TGG 0.273928 4.4:-30544808 None:intergenic
GACCATAGATGGTGCCTTCC+TGG 0.298042 4.4:+30544286 MS.gene56532:CDS
GGCTTTCATTGGCTTGGTTT+TGG 0.300511 4.4:+30543900 MS.gene56532:CDS
GTAGGTGGGGCTTGTGGATA+TGG 0.300951 4.4:+30544157 MS.gene56532:intron
GGATATCTATGTCAAGAAAC+TGG 0.302501 4.4:+30544700 MS.gene56532:CDS
GTGGCTCTTGCTATGAAATT+AGG 0.339101 4.4:+30544254 MS.gene56532:CDS
TTCCATACCCTTGACTGTAT+AGG 0.380723 4.4:-30544183 None:intergenic
TCCAATAATGTTGCTCCAGC+TGG 0.392823 4.4:+30544807 MS.gene56532:CDS
AAACAGGAAATGGCTTTCAT+TGG 0.394019 4.4:+30543889 None:intergenic
AACCAGGAAGGCACCATCTA+TGG 0.400538 4.4:-30544288 None:intergenic
TTGTTTGGTAAGGCATTGTT+TGG 0.404496 4.4:-30544343 None:intergenic
CAAGAAACTGGGGACAAAAC+TGG 0.410611 4.4:+30544712 MS.gene56532:CDS
ATCACAAACGTTGGTGGTGC+TGG 0.417253 4.4:+30544636 MS.gene56532:CDS
TGCATCACTTCCTCCATAGA+AGG 0.441161 4.4:-30543989 None:intergenic
GGAAATGGCTTTCATTGGCT+TGG 0.445049 4.4:+30543894 None:intergenic
CAAAGTAACAATTATCTCAA+TGG 0.478172 4.4:+30544735 MS.gene56532:CDS
TCTGTTTATGCTTATGGTGG+AGG 0.479574 4.4:+30543946 MS.gene56532:CDS
AGGGTTTCATGCAGAAGAAA+GGG 0.483984 4.4:+30544564 MS.gene56532:intron
CAACGTTTGTGATAAGAACT+AGG 0.485658 4.4:-30544626 None:intergenic
TCCTCTGTTTATGCTTATGG+TGG 0.486226 4.4:+30543943 MS.gene56532:CDS
TAGGGTTTCATGCAGAAGAA+AGG 0.496633 4.4:+30544563 MS.gene56532:intron
GGTATAAGGTTCACTATCAA+TGG 0.504908 4.4:+30544588 MS.gene56532:CDS
AGTGATGCATCAGGAACAAT+GGG 0.509766 4.4:+30544003 MS.gene56532:CDS
TATGGAGGAAGTGATGCATC+AGG 0.510885 4.4:+30543994 MS.gene56532:CDS
CGTATTGCTCAATACAAAGC+TGG 0.513556 4.4:+30544427 MS.gene56532:CDS
ACCACCTTGATTGTTTGGTA+AGG 0.522218 4.4:-30544353 None:intergenic
TTTGGTAAGGCATTGTTTGG+AGG 0.522270 4.4:-30544340 None:intergenic
GTTGAGCAAGATCAAAATGT+TGG 0.527634 4.4:-30544393 None:intergenic
TTTAAGGTTACAACTAGTGA+TGG 0.529102 4.4:+30544771 MS.gene56532:CDS
AACCTATACAGTCAAGGGTA+TGG 0.535142 4.4:+30544181 MS.gene56532:CDS
TACATGCTGCATCCATCAAA+GGG 0.543754 4.4:+30544664 MS.gene56532:CDS
TTCAACAATGGCTTAAGCTG+TGG 0.546607 4.4:+30544235 MS.gene56532:CDS
GATCAAAATGTTGGAGAGGA+GGG 0.550651 4.4:-30544384 None:intergenic
TTTATGCTTATGGTGGAGGA+TGG 0.551717 4.4:+30543950 MS.gene56532:CDS
AGGTACCTTCTAAAATCTAC+AGG 0.552530 4.4:-30544457 None:intergenic
GTTCTTATCACAAACGTTGG+TGG 0.552684 4.4:+30544630 MS.gene56532:CDS
GTTTGGAGGACAGAAATTTG+TGG 0.554497 4.4:-30544326 None:intergenic
AAGTGATGCATCAGGAACAA+TGG 0.564601 4.4:+30544002 MS.gene56532:CDS
GTACATGCTGCATCCATCAA+AGG 0.567778 4.4:+30544663 MS.gene56532:CDS
TGACCAAAAGACCATCCAGC+TGG 0.568697 4.4:-30544822 None:intergenic
GATATCTATGTCAAGAAACT+GGG 0.570919 4.4:+30544701 MS.gene56532:CDS
GTGCCTTCCTGGTTCCATTG+TGG 0.583469 4.4:+30544297 MS.gene56532:CDS
AGCATAAACAGAGGAGAACA+TGG 0.584006 4.4:-30543935 None:intergenic
AGATCAAAATGTTGGAGAGG+AGG 0.586254 4.4:-30544385 None:intergenic
ATAATGTTGCTCCAGCTGGA+TGG 0.590574 4.4:+30544811 MS.gene56532:CDS
TATGGTAACCTATACAGTCA+AGG 0.596340 4.4:+30544175 MS.gene56532:CDS
GCAGTAACCACAATGGAACC+AGG 0.598129 4.4:-30544304 None:intergenic
ATGGTAACCTATACAGTCAA+GGG 0.598519 4.4:+30544176 MS.gene56532:CDS
TACCAAACAATCAAGGTGGT+TGG 0.598522 4.4:+30544356 MS.gene56532:CDS
GGTTTCATGCAGAAGAAAGG+GGG 0.603010 4.4:+30544566 MS.gene56532:CDS
AATGCCTTACCAAACAATCA+AGG 0.612141 4.4:+30544349 MS.gene56532:CDS
GGGTTTCATGCAGAAGAAAG+GGG 0.623572 4.4:+30544565 MS.gene56532:intron
GAGGATTTGAGCACCAGTGT+AGG 0.625146 4.4:-30544847 None:intergenic
GGTGTGCTAATGACCATAGA+TGG 0.625411 4.4:+30544275 MS.gene56532:CDS
GCACATGCTACCTTCTATGG+AGG 0.632518 4.4:+30543979 MS.gene56532:CDS
CTAGTTCTTATCACAAACGT+TGG 0.640263 4.4:+30544627 MS.gene56532:CDS
GCAAGATCAAAATGTTGGAG+AGG 0.641783 4.4:-30544388 None:intergenic
TAACCACAATGGAACCAGGA+AGG 0.677839 4.4:-30544300 None:intergenic
ATATCTATGTCAAGAAACTG+GGG 0.706710 4.4:+30544702 MS.gene56532:CDS
ATTTGTGGCAGTAACCACAA+TGG 0.711966 4.4:-30544311 None:intergenic
GCCTTACCAAACAATCAAGG+TGG 0.713350 4.4:+30544352 MS.gene56532:CDS
GTTTCATGCAGAAGAAAGGG+GGG 0.725105 4.4:+30544567 MS.gene56532:CDS
TCCACCATAAGCATAAACAG+AGG 0.731325 4.4:-30543944 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! ATTTTCACTTTTTTTGTATT+TGG + chr4.4:30544502-30544521 MS.gene56532:intron 15.0%
!! AAAATCAAAACATTATTTCG+TGG - chr4.4:30544053-30544072 None:intergenic 20.0%
!! ATATAAAAATGACAAGAGAA+AGG - chr4.4:30544480-30544499 None:intergenic 20.0%
! AAACAACAAAATCAGTGAAA+TGG - chr4.4:30544132-30544151 None:intergenic 25.0%
! AACAACAAAATCAGTGAAAT+GGG - chr4.4:30544131-30544150 None:intergenic 25.0%
! CAAAGTAACAATTATCTCAA+TGG + chr4.4:30544735-30544754 MS.gene56532:CDS 25.0%
!! CGTGGTTTTTATTGAAAAAA+TGG - chr4.4:30544035-30544054 None:intergenic 25.0%
!!! GATTTGTGTTTTTATTGTGT+TGG + chr4.4:30544533-30544552 MS.gene56532:intron 25.0%
!!! GATTTTGTTGTTTTTTGTGT+AGG + chr4.4:30544139-30544158 MS.gene56532:intron 25.0%
ATATCTATGTCAAGAAACTG+GGG + chr4.4:30544702-30544721 MS.gene56532:CDS 30.0%
GATATCTATGTCAAGAAACT+GGG + chr4.4:30544701-30544720 MS.gene56532:CDS 30.0%
! TGGTCAAAGTTTGTCTTTTA+AGG + chr4.4:30544755-30544774 MS.gene56532:CDS 30.0%
! TTGTTCCTGTAGATTTTAGA+AGG + chr4.4:30544452-30544471 MS.gene56532:CDS 30.0%
! TTTAAGGTTACAACTAGTGA+TGG + chr4.4:30544771-30544790 MS.gene56532:CDS 30.0%
!!! TATTGTGTTGGTTTTGTGTA+GGG + chr4.4:30544545-30544564 MS.gene56532:intron 30.0%
!!! TTATTGTGTTGGTTTTGTGT+AGG + chr4.4:30544544-30544563 MS.gene56532:intron 30.0%
!!! TTGTTGTTTTTTGTGTAGGT+GGG + chr4.4:30544143-30544162 MS.gene56532:intron 30.0%
!!! TTGTTTTTTGCTTCAAGATC+TGG - chr4.4:30544104-30544123 None:intergenic 30.0%
!!! TTTGTTGTTTTTTGTGTAGG+TGG + chr4.4:30544142-30544161 MS.gene56532:intron 30.0%
AATGCCTTACCAAACAATCA+AGG + chr4.4:30544349-30544368 MS.gene56532:CDS 35.0%
ATGGTAACCTATACAGTCAA+GGG + chr4.4:30544176-30544195 MS.gene56532:CDS 35.0%
CAACGTTTGTGATAAGAACT+AGG - chr4.4:30544629-30544648 None:intergenic 35.0%
GGATATCTATGTCAAGAAAC+TGG + chr4.4:30544700-30544719 MS.gene56532:CDS 35.0%
GGTATAAGGTTCACTATCAA+TGG + chr4.4:30544588-30544607 MS.gene56532:CDS 35.0%
GTTGAGCAAGATCAAAATGT+TGG - chr4.4:30544396-30544415 None:intergenic 35.0%
TATGGTAACCTATACAGTCA+AGG + chr4.4:30544175-30544194 MS.gene56532:CDS 35.0%
TGAGCAATACGAAGAAAAAC+AGG - chr4.4:30544418-30544437 None:intergenic 35.0%
TTCTCCTCTGTTTATGCTTA+TGG + chr4.4:30543940-30543959 MS.gene56532:CDS 35.0%
! AGTACAGCACTTTTCAACAA+TGG + chr4.4:30544223-30544242 MS.gene56532:CDS 35.0%
! CTAGTTCTTATCACAAACGT+TGG + chr4.4:30544627-30544646 MS.gene56532:CDS 35.0%
!! AGGTACCTTCTAAAATCTAC+AGG - chr4.4:30544460-30544479 None:intergenic 35.0%
!! TTGTTTGGTAAGGCATTGTT+TGG - chr4.4:30544346-30544365 None:intergenic 35.0%
!!! TGTTGTTTTTTGTGTAGGTG+GGG + chr4.4:30544144-30544163 MS.gene56532:intron 35.0%
AACCTATACAGTCAAGGGTA+TGG + chr4.4:30544181-30544200 MS.gene56532:CDS 40.0%
AAGTGATGCATCAGGAACAA+TGG + chr4.4:30544002-30544021 MS.gene56532:CDS 40.0%
AATGCACATGCTACCTTCTA+TGG + chr4.4:30543976-30543995 MS.gene56532:CDS 40.0%
ACCACCTTGATTGTTTGGTA+AGG - chr4.4:30544356-30544375 None:intergenic 40.0%
AGATCAAAATGTTGGAGAGG+AGG - chr4.4:30544388-30544407 None:intergenic 40.0%
AGCATAAACAGAGGAGAACA+TGG - chr4.4:30543938-30543957 None:intergenic 40.0%
AGGGTTTCATGCAGAAGAAA+GGG + chr4.4:30544564-30544583 MS.gene56532:intron 40.0%
AGTGATGCATCAGGAACAAT+GGG + chr4.4:30544003-30544022 MS.gene56532:CDS 40.0%
ATTTGTGGCAGTAACCACAA+TGG - chr4.4:30544314-30544333 None:intergenic 40.0%
CGTATTGCTCAATACAAAGC+TGG + chr4.4:30544427-30544446 MS.gene56532:CDS 40.0%
GATCAAAATGTTGGAGAGGA+GGG - chr4.4:30544387-30544406 None:intergenic 40.0%
GCAAGATCAAAATGTTGGAG+AGG - chr4.4:30544391-30544410 None:intergenic 40.0%
GTTCTTATCACAAACGTTGG+TGG + chr4.4:30544630-30544649 MS.gene56532:CDS 40.0%
GTTTGGAGGACAGAAATTTG+TGG - chr4.4:30544329-30544348 None:intergenic 40.0%
TACATGCTGCATCCATCAAA+GGG + chr4.4:30544664-30544683 MS.gene56532:CDS 40.0%
TACCAAACAATCAAGGTGGT+TGG + chr4.4:30544356-30544375 MS.gene56532:CDS 40.0%
TAGGGTTTCATGCAGAAGAA+AGG + chr4.4:30544563-30544582 MS.gene56532:intron 40.0%
TCAAAGGGTCAAAAACTGGT+TGG + chr4.4:30544679-30544698 MS.gene56532:CDS 40.0%
TCCACCATAAGCATAAACAG+AGG - chr4.4:30543947-30543966 None:intergenic 40.0%
TCCATCAAAGGGTCAAAAAC+TGG + chr4.4:30544675-30544694 MS.gene56532:CDS 40.0%
TCCTCTGTTTATGCTTATGG+TGG + chr4.4:30543943-30543962 MS.gene56532:CDS 40.0%
TCTGTTTATGCTTATGGTGG+AGG + chr4.4:30543946-30543965 MS.gene56532:CDS 40.0%
TTCAACAATGGCTTAAGCTG+TGG + chr4.4:30544235-30544254 MS.gene56532:CDS 40.0%
TTCCATACCCTTGACTGTAT+AGG - chr4.4:30544186-30544205 None:intergenic 40.0%
! ACCAGTTTTTGACCCTTTGA+TGG - chr4.4:30544679-30544698 None:intergenic 40.0%
! GTGGCTCTTGCTATGAAATT+AGG + chr4.4:30544254-30544273 MS.gene56532:CDS 40.0%
! TCTTTTGGTCAAACCTACAC+TGG + chr4.4:30544834-30544853 MS.gene56532:CDS 40.0%
! TTTATGCTTATGGTGGAGGA+TGG + chr4.4:30543950-30543969 MS.gene56532:CDS 40.0%
!! TTTGGTAAGGCATTGTTTGG+AGG - chr4.4:30544343-30544362 None:intergenic 40.0%
ATAATGTTGCTCCAGCTGGA+TGG + chr4.4:30544811-30544830 MS.gene56532:CDS 45.0%
CAAGAAACTGGGGACAAAAC+TGG + chr4.4:30544712-30544731 MS.gene56532:CDS 45.0%
CACCAACCACCTTGATTGTT+TGG - chr4.4:30544361-30544380 None:intergenic 45.0%
GCCTTACCAAACAATCAAGG+TGG + chr4.4:30544352-30544371 MS.gene56532:CDS 45.0%
GGGTTTCATGCAGAAGAAAG+GGG + chr4.4:30544565-30544584 MS.gene56532:intron 45.0%
GGTGTGCTAATGACCATAGA+TGG + chr4.4:30544275-30544294 MS.gene56532:CDS 45.0%
GGTTTCATGCAGAAGAAAGG+GGG + chr4.4:30544566-30544585 MS.gene56532:CDS 45.0%
GTACATGCTGCATCCATCAA+AGG + chr4.4:30544663-30544682 MS.gene56532:CDS 45.0%
GTTTCATGCAGAAGAAAGGG+GGG + chr4.4:30544567-30544586 MS.gene56532:CDS 45.0%
TAACCACAATGGAACCAGGA+AGG - chr4.4:30544303-30544322 None:intergenic 45.0%
TATGGAGGAAGTGATGCATC+AGG + chr4.4:30543994-30544013 MS.gene56532:CDS 45.0%
TCCAATAATGTTGCTCCAGC+TGG + chr4.4:30544807-30544826 MS.gene56532:CDS 45.0%
TCCAGCTGGAGCAACATTAT+TGG - chr4.4:30544811-30544830 None:intergenic 45.0%
TGCATCACTTCCTCCATAGA+AGG - chr4.4:30543992-30544011 None:intergenic 45.0%
!!! GGCTTTCATTGGCTTGGTTT+TGG + chr4.4:30543900-30543919 MS.gene56532:CDS 45.0%
GAGGATTTGAGCACCAGTGT+AGG - chr4.4:30544850-30544869 None:intergenic 50.0%
GCACATGCTACCTTCTATGG+AGG + chr4.4:30543979-30543998 MS.gene56532:CDS 50.0%
GCAGTAACCACAATGGAACC+AGG - chr4.4:30544307-30544326 None:intergenic 50.0%
TGACCAAAAGACCATCCAGC+TGG - chr4.4:30544825-30544844 None:intergenic 50.0%
! GCAGAAGAAAGGGGGGTATA+AGG + chr4.4:30544574-30544593 MS.gene56532:CDS 50.0%
! TTTTGTGTAGGTGGGGCTTG+TGG + chr4.4:30544151-30544170 MS.gene56532:intron 50.0%
!! AACCAGGAAGGCACCATCTA+TGG - chr4.4:30544291-30544310 None:intergenic 50.0%
!! ATCACAAACGTTGGTGGTGC+TGG + chr4.4:30544636-30544655 MS.gene56532:CDS 50.0%
GACCATAGATGGTGCCTTCC+TGG + chr4.4:30544286-30544305 MS.gene56532:CDS 55.0%
GTAGGTGGGGCTTGTGGATA+TGG + chr4.4:30544157-30544176 MS.gene56532:intron 55.0%
GTGCCTTCCTGGTTCCATTG+TGG + chr4.4:30544297-30544316 MS.gene56532:CDS 55.0%
! GCTCCAGCTGGATGGTCTTT+TGG + chr4.4:30544819-30544838 MS.gene56532:CDS 55.0%


Chromosome Type Strat End Strand Name
chr4.4 gene 30543898 30544872 30543898 ID=MS.gene56532
chr4.4 mRNA 30543898 30544872 30543898 ID=MS.gene56532.t1;Parent=MS.gene56532
chr4.4 exon 30543898 30544024 30543898 ID=MS.gene56532.t1.exon1;Parent=MS.gene56532.t1
chr4.4 CDS 30543898 30544024 30543898 ID=cds.MS.gene56532.t1;Parent=MS.gene56532.t1
chr4.4 exon 30544161 30544473 30544161 ID=MS.gene56532.t1.exon2;Parent=MS.gene56532.t1
chr4.4 CDS 30544161 30544473 30544161 ID=cds.MS.gene56532.t1;Parent=MS.gene56532.t1
chr4.4 exon 30544566 30544872 30544566 ID=MS.gene56532.t1.exon3;Parent=MS.gene56532.t1
chr4.4 CDS 30544566 30544872 30544566 ID=cds.MS.gene56532.t1;Parent=MS.gene56532.t1
Gene Sequence

>MS.gene56532

ATGGCTTTCATTGGCTTGGTTTTGGTGTGTTCTCTTACCATGTTCTCCTCTGTTTATGCTTATGGTGGAGGATGGACTAATGCACATGCTACCTTCTATGGAGGAAGTGATGCATCAGGAACAATGGGTATGCACCATTTTTTCAATAAAAACCACGAAATAATGTTTTGATTTTTATGCTTCTTTGTAAAAAAAAAAAAAAACCAGATCTTGAAGCAAAAAACAAGTAACCCATTTCACTGATTTTGTTGTTTTTTGTGTAGGTGGGGCTTGTGGATATGGTAACCTATACAGTCAAGGGTATGGAACAAACACTGCTGCTTTGAGTACAGCACTTTTCAACAATGGCTTAAGCTGTGGCTCTTGCTATGAAATTAGGTGTGCTAATGACCATAGATGGTGCCTTCCTGGTTCCATTGTGGTTACTGCCACAAATTTCTGTCCTCCAAACAATGCCTTACCAAACAATCAAGGTGGTTGGTGTAACCCTCCTCTCCAACATTTTGATCTTGCTCAACCTGTTTTTCTTCGTATTGCTCAATACAAAGCTGGAATTGTTCCTGTAGATTTTAGAAGGTACCTTTCTCTTGTCATTTTTATATAAATTTTCACTTTTTTTGTATTTGGTATATACTGATTTGTGTTTTTATTGTGTTGGTTTTGTGTAGGGTTTCATGCAGAAGAAAGGGGGGTATAAGGTTCACTATCAATGGACATTCTTACTTTAACCTAGTTCTTATCACAAACGTTGGTGGTGCTGGAGATGTACATGCTGCATCCATCAAAGGGTCAAAAACTGGTTGGATATCTATGTCAAGAAACTGGGGACAAAACTGGCAAAGTAACAATTATCTCAATGGTCAAAGTTTGTCTTTTAAGGTTACAACTAGTGATGGTCGTACTGTTGTTTCCAATAATGTTGCTCCAGCTGGATGGTCTTTTGGTCAAACCTACACTGGTGCTCAAATCCTCTAG
Protein sequence

>MS.gene56532.t1

MAFIGLVLVCSLTMFSSVYAYGGGWTNAHATFYGGSDASGTMGGACGYGNLYSQGYGTNTAALSTALFNNGLSCGSCYEIRCANDHRWCLPGSIVVTATNFCPPNNALPNNQGGWCNPPLQHFDLAQPVFLRIAQYKAGIVPVDFRRVSCRRKGGIRFTINGHSYFNLVLITNVGGAGDVHAASIKGSKTGWISMSRNWGQNWQSNNYLNGQSLSFKVTTSDGRTVVSNNVAPAGWSFGQTYTGAQIL