Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene56535.t1 | XP_003607747.3 | 84.8 | 171 | 10 | 1 | 1 | 155 | 10 | 180 | 8.60E-72 | 279.6 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene56535.t1 | Q93Z27 | 41.6 | 113 | 63 | 2 | 45 | 154 | 91 | 203 | 5.2e-17 | 89.0 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene56535.t1 | A0A396IB26 | 84.8 | 171 | 10 | 1 | 1 | 155 | 10 | 180 | 6.2e-72 | 279.6 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene051544 | MS.gene56535 | 0.812084 | 5.07E-51 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene56535.t1 | MTR_4g082050 | 94.286 | 140 | 8 | 0 | 16 | 155 | 4 | 143 | 1.21e-89 | 257 |
| MS.gene56535.t1 | MTR_5g025690 | 47.059 | 119 | 59 | 3 | 36 | 151 | 79 | 196 | 3.63e-23 | 90.5 |
| MS.gene56535.t1 | MTR_7g011010 | 45.968 | 124 | 63 | 3 | 31 | 151 | 76 | 198 | 1.84e-22 | 89.0 |
| MS.gene56535.t1 | MTR_4g127560 | 47.009 | 117 | 59 | 2 | 41 | 154 | 81 | 197 | 3.10e-22 | 88.2 |
| MS.gene56535.t1 | MTR_4g067270 | 50.000 | 66 | 31 | 1 | 88 | 151 | 77 | 142 | 4.30e-14 | 65.9 |
| MS.gene56535.t1 | MTR_7g075040 | 51.515 | 66 | 31 | 1 | 88 | 152 | 127 | 192 | 8.72e-14 | 65.9 |
| MS.gene56535.t1 | MTR_2g098890 | 52.459 | 61 | 27 | 1 | 93 | 151 | 143 | 203 | 3.23e-13 | 64.7 |
| MS.gene56535.t1 | MTR_1g032070 | 50.000 | 70 | 34 | 1 | 87 | 155 | 117 | 186 | 3.32e-13 | 64.3 |
| MS.gene56535.t1 | MTR_3g109320 | 36.885 | 122 | 67 | 2 | 39 | 150 | 57 | 178 | 1.04e-12 | 62.8 |
| MS.gene56535.t1 | MTR_0168s0120 | 44.595 | 74 | 41 | 0 | 77 | 150 | 37 | 110 | 1.29e-12 | 60.8 |
| MS.gene56535.t1 | MTR_6g025320 | 44.595 | 74 | 41 | 0 | 77 | 150 | 37 | 110 | 1.29e-12 | 60.8 |
| MS.gene56535.t1 | MTR_5g088320 | 44.595 | 74 | 41 | 0 | 77 | 150 | 73 | 146 | 2.32e-12 | 61.2 |
| MS.gene56535.t1 | MTR_7g087610 | 44.595 | 74 | 41 | 0 | 77 | 150 | 73 | 146 | 2.37e-12 | 61.2 |
| MS.gene56535.t1 | MTR_6g025320 | 44.595 | 74 | 41 | 0 | 77 | 150 | 73 | 146 | 2.42e-12 | 61.2 |
| MS.gene56535.t1 | MTR_7g090450 | 49.231 | 65 | 32 | 1 | 88 | 151 | 149 | 213 | 5.27e-12 | 61.6 |
| MS.gene56535.t1 | MTR_3g089090 | 35.455 | 110 | 68 | 2 | 44 | 151 | 31 | 139 | 5.99e-12 | 60.1 |
| MS.gene56535.t1 | MTR_3g089070 | 35.455 | 110 | 68 | 2 | 44 | 151 | 32 | 140 | 6.04e-12 | 60.1 |
| MS.gene56535.t1 | MTR_5g017550 | 43.333 | 90 | 47 | 2 | 64 | 150 | 93 | 181 | 8.99e-12 | 60.5 |
| MS.gene56535.t1 | MTR_4g086260 | 41.429 | 70 | 41 | 0 | 84 | 153 | 123 | 192 | 9.89e-12 | 60.5 |
| MS.gene56535.t1 | MTR_3g067610 | 31.852 | 135 | 82 | 3 | 29 | 153 | 4 | 138 | 1.02e-11 | 59.3 |
| MS.gene56535.t1 | MTR_8g107110 | 31.579 | 152 | 88 | 4 | 2 | 150 | 19 | 157 | 1.44e-11 | 59.3 |
| MS.gene56535.t1 | MTR_1g076650 | 40.541 | 74 | 44 | 0 | 77 | 150 | 73 | 146 | 1.44e-11 | 59.3 |
| MS.gene56535.t1 | MTR_5g017510 | 36.364 | 121 | 62 | 3 | 44 | 150 | 63 | 182 | 3.95e-11 | 58.9 |
| MS.gene56535.t1 | MTR_5g017560 | 36.364 | 121 | 62 | 3 | 44 | 150 | 63 | 182 | 3.95e-11 | 58.9 |
| MS.gene56535.t1 | MTR_8g036075 | 37.600 | 125 | 61 | 5 | 34 | 151 | 78 | 192 | 5.38e-11 | 58.5 |
| MS.gene56535.t1 | MTR_7g089760 | 47.541 | 61 | 30 | 1 | 93 | 151 | 82 | 142 | 8.04e-11 | 57.4 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene56535.t1 | AT5G39670 | 41.593 | 113 | 63 | 2 | 45 | 154 | 91 | 203 | 5.42e-22 | 87.8 |
| MS.gene56535.t1 | AT3G29000 | 46.429 | 112 | 55 | 4 | 46 | 154 | 84 | 193 | 1.43e-19 | 81.3 |
| MS.gene56535.t1 | AT3G47480 | 53.623 | 69 | 31 | 1 | 88 | 155 | 115 | 183 | 1.41e-15 | 70.5 |
| MS.gene56535.t1 | AT1G05990 | 50.000 | 64 | 30 | 1 | 90 | 151 | 80 | 143 | 2.49e-13 | 63.9 |
| MS.gene56535.t1 | AT4G03290 | 50.769 | 65 | 30 | 1 | 90 | 152 | 82 | 146 | 2.51e-13 | 63.9 |
| MS.gene56535.t1 | AT3G07490 | 49.180 | 61 | 29 | 1 | 93 | 151 | 82 | 142 | 4.88e-13 | 63.2 |
| MS.gene56535.t1 | AT1G66410 | 44.595 | 74 | 41 | 0 | 77 | 150 | 37 | 110 | 6.19e-13 | 62.0 |
| MS.gene56535.t1 | AT1G66410 | 44.595 | 74 | 41 | 0 | 77 | 150 | 37 | 110 | 6.19e-13 | 62.0 |
| MS.gene56535.t1 | AT5G44460 | 47.887 | 71 | 35 | 1 | 83 | 151 | 105 | 175 | 8.22e-13 | 63.2 |
| MS.gene56535.t1 | AT5G37780 | 44.595 | 74 | 41 | 0 | 77 | 150 | 73 | 146 | 1.16e-12 | 62.0 |
| MS.gene56535.t1 | AT1G66410 | 44.595 | 74 | 41 | 0 | 77 | 150 | 73 | 146 | 1.16e-12 | 62.0 |
| MS.gene56535.t1 | AT3G43810 | 44.595 | 74 | 41 | 0 | 77 | 150 | 37 | 110 | 1.35e-12 | 60.8 |
| MS.gene56535.t1 | AT3G43810 | 44.595 | 74 | 41 | 0 | 77 | 150 | 37 | 110 | 1.35e-12 | 60.8 |
| MS.gene56535.t1 | AT1G66410 | 44.595 | 74 | 41 | 0 | 77 | 150 | 83 | 156 | 1.83e-12 | 61.6 |
| MS.gene56535.t1 | AT5G21274 | 44.595 | 74 | 41 | 0 | 77 | 150 | 73 | 146 | 2.14e-12 | 61.2 |
| MS.gene56535.t1 | AT5G37780 | 44.595 | 74 | 41 | 0 | 77 | 150 | 60 | 133 | 2.21e-12 | 61.2 |
| MS.gene56535.t1 | AT3G56800 | 44.595 | 74 | 41 | 0 | 77 | 150 | 73 | 146 | 2.51e-12 | 61.2 |
| MS.gene56535.t1 | AT2G27030 | 44.595 | 74 | 41 | 0 | 77 | 150 | 73 | 146 | 2.51e-12 | 61.2 |
| MS.gene56535.t1 | AT2G41110 | 44.595 | 74 | 41 | 0 | 77 | 150 | 73 | 146 | 2.51e-12 | 61.2 |
| MS.gene56535.t1 | AT3G43810 | 44.595 | 74 | 41 | 0 | 77 | 150 | 73 | 146 | 2.53e-12 | 61.2 |
| MS.gene56535.t1 | AT5G37780 | 44.595 | 74 | 41 | 0 | 77 | 150 | 99 | 172 | 2.97e-12 | 61.6 |
| MS.gene56535.t1 | AT5G37780 | 44.595 | 74 | 41 | 0 | 77 | 150 | 88 | 161 | 3.29e-12 | 61.2 |
| MS.gene56535.t1 | AT2G27030 | 44.595 | 74 | 41 | 0 | 77 | 150 | 73 | 146 | 3.32e-12 | 61.6 |
| MS.gene56535.t1 | AT2G41110 | 44.595 | 74 | 41 | 0 | 77 | 150 | 85 | 158 | 3.72e-12 | 60.8 |
| MS.gene56535.t1 | AT2G43290 | 45.455 | 66 | 34 | 1 | 88 | 151 | 142 | 207 | 5.13e-12 | 61.6 |
| MS.gene56535.t1 | AT4G20780 | 50.769 | 65 | 30 | 1 | 89 | 151 | 120 | 184 | 1.03e-11 | 60.5 |
| MS.gene56535.t1 | AT3G43810 | 44.595 | 74 | 41 | 0 | 77 | 150 | 73 | 146 | 1.14e-11 | 60.5 |
| MS.gene56535.t1 | AT1G24620 | 48.438 | 64 | 33 | 0 | 87 | 150 | 108 | 171 | 1.52e-11 | 59.7 |
| MS.gene56535.t1 | AT2G41100 | 32.857 | 140 | 78 | 4 | 31 | 155 | 29 | 167 | 1.82e-11 | 61.2 |
| MS.gene56535.t1 | AT2G41100 | 32.857 | 140 | 78 | 4 | 31 | 155 | 29 | 167 | 1.82e-11 | 61.2 |
| MS.gene56535.t1 | AT1G73630 | 32.110 | 109 | 67 | 2 | 42 | 150 | 49 | 150 | 1.87e-11 | 59.3 |
| MS.gene56535.t1 | AT2G41100 | 43.478 | 69 | 39 | 0 | 87 | 155 | 64 | 132 | 2.08e-11 | 60.8 |
| MS.gene56535.t1 | AT2G41100 | 43.478 | 69 | 39 | 0 | 87 | 155 | 64 | 132 | 2.08e-11 | 60.8 |
| MS.gene56535.t1 | AT2G41100 | 43.478 | 69 | 39 | 0 | 87 | 155 | 64 | 132 | 2.08e-11 | 60.8 |
| MS.gene56535.t1 | AT4G12860 | 47.541 | 61 | 30 | 1 | 93 | 151 | 82 | 142 | 2.21e-11 | 58.9 |
| MS.gene56535.t1 | AT1G66400 | 38.667 | 75 | 46 | 0 | 76 | 150 | 77 | 151 | 6.00e-11 | 57.8 |
Find 30 sgRNAs with CRISPR-Local
Find 32 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| ATTGCTCATATGTTTGAAAA+TGG | 0.169368 | 4.4:-30625353 | MS.gene56535:CDS |
| GTTTGAAAATGGGGTTAGTT+TGG | 0.294711 | 4.4:-30625342 | MS.gene56535:CDS |
| GAGGATGATCCAGGGGAATT+TGG | 0.310466 | 4.4:-30625389 | MS.gene56535:CDS |
| ATTAATGCAGTTGATCAAAA+TGG | 0.313664 | 4.4:-30625194 | MS.gene56535:CDS |
| CCTTGTAGCATAAATATGTT+TGG | 0.318327 | 4.4:-30625680 | None:intergenic |
| CTATGAGTTTGTTGTGTTGA+TGG | 0.336088 | 4.4:-30625153 | MS.gene56535:CDS |
| AAGAGAGTCTTGTGTTGTTT+GGG | 0.336625 | 4.4:-30625251 | MS.gene56535:CDS |
| GAAGAGAGTCTTGTGTTGTT+TGG | 0.378049 | 4.4:-30625252 | MS.gene56535:CDS |
| TTGCTCATATGTTTGAAAAT+GGG | 0.395795 | 4.4:-30625352 | MS.gene56535:CDS |
| GTGTTGATGGAACAAAGTTT+TGG | 0.423873 | 4.4:-30625140 | MS.gene56535:CDS |
| CATTGTTGTGATGGAAAAGC+TGG | 0.444649 | 4.4:-30625432 | MS.gene56535:CDS |
| TAATCTTGAACAAGTGAAGC+AGG | 0.448279 | 4.4:-30625504 | MS.gene56535:CDS |
| CTTGTAGCATAAATATGTTT+GGG | 0.457566 | 4.4:-30625679 | None:intergenic |
| TGCTCATATGTTTGAAAATG+GGG | 0.468776 | 4.4:-30625351 | MS.gene56535:CDS |
| GAGTGTGATGAGGATGATCC+AGG | 0.478363 | 4.4:-30625398 | MS.gene56535:CDS |
| ATTTAAGTTTAACGTAACAT+AGG | 0.483839 | 4.4:+30625630 | None:intergenic |
| TGTTCATCACCAAATTCCCC+TGG | 0.490165 | 4.4:+30625380 | None:intergenic |
| GTGTTTGATGAAAACAAAGA+TGG | 0.498276 | 4.4:-30625296 | MS.gene56535:CDS |
| AGAAGAAATCATTGTTGTGA+TGG | 0.498467 | 4.4:-30625441 | MS.gene56535:CDS |
| GGAAAAGCTGGGTTTAAAAG+TGG | 0.516441 | 4.4:-30625420 | MS.gene56535:CDS |
| AAACAAAGATGGGTTTATAG+AGG | 0.531105 | 4.4:-30625285 | MS.gene56535:CDS |
| TGTTTGATGAAAACAAAGAT+GGG | 0.535462 | 4.4:-30625295 | MS.gene56535:CDS |
| GCAGTTGATCAAAATGGAGA+TGG | 0.549331 | 4.4:-30625188 | MS.gene56535:CDS |
| AATCTTGAACAAGTGAAGCA+GGG | 0.597395 | 4.4:-30625503 | MS.gene56535:CDS |
| TCAACACAACAAACTCATAG+TGG | 0.604767 | 4.4:+30625156 | None:intergenic |
| ATTGTTGTGATGGAAAAGCT+GGG | 0.609141 | 4.4:-30625431 | MS.gene56535:CDS |
| TTGAATAGCTGCATTCAAAG+TGG | 0.646037 | 4.4:-30625479 | MS.gene56535:CDS |
| TTTAAAAGTGGAGTGTGATG+AGG | 0.674320 | 4.4:-30625408 | MS.gene56535:CDS |
| AGTGTGATGAGGATGATCCA+GGG | 0.694086 | 4.4:-30625397 | MS.gene56535:CDS |
| GTGTGATGAGGATGATCCAG+GGG | 0.709283 | 4.4:-30625396 | MS.gene56535:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | CATAAAAAATTAAAATTGAA+AGG | + | chr4.4:30625201-30625220 | None:intergenic | 10.0% |
| !!! | ATGTTTTCTTTTTCTACAAT+TGG | - | chr4.4:30625239-30625258 | MS.gene56535:CDS | 20.0% |
| !!! | ATTTAAGTTTAACGTAACAT+AGG | + | chr4.4:30625174-30625193 | None:intergenic | 20.0% |
| ! | ATTAATGCAGTTGATCAAAA+TGG | - | chr4.4:30625607-30625626 | MS.gene56535:intron | 25.0% |
| ! | ATTGCTCATATGTTTGAAAA+TGG | - | chr4.4:30625448-30625467 | MS.gene56535:CDS | 25.0% |
| ! | TGTTTGATGAAAACAAAGAT+GGG | - | chr4.4:30625506-30625525 | MS.gene56535:CDS | 25.0% |
| ! | TTGCTCATATGTTTGAAAAT+GGG | - | chr4.4:30625449-30625468 | MS.gene56535:CDS | 25.0% |
| AGAAGAAATCATTGTTGTGA+TGG | - | chr4.4:30625360-30625379 | MS.gene56535:CDS | 30.0% | |
| GTGTTTGATGAAAACAAAGA+TGG | - | chr4.4:30625505-30625524 | MS.gene56535:CDS | 30.0% | |
| TGCTCATATGTTTGAAAATG+GGG | - | chr4.4:30625450-30625469 | MS.gene56535:CDS | 30.0% | |
| ! | AAACAAAGATGGGTTTATAG+AGG | - | chr4.4:30625516-30625535 | MS.gene56535:CDS | 30.0% |
| !!! | TTTTTTATGTGCACACATTG+TGG | - | chr4.4:30625212-30625231 | MS.gene56535:CDS | 30.0% |
| AATCTTGAACAAGTGAAGCA+GGG | - | chr4.4:30625298-30625317 | MS.gene56535:CDS | 35.0% | |
| TAATCTTGAACAAGTGAAGC+AGG | - | chr4.4:30625297-30625316 | MS.gene56535:CDS | 35.0% | |
| TCAACACAACAAACTCATAG+TGG | + | chr4.4:30625648-30625667 | None:intergenic | 35.0% | |
| TTGAATAGCTGCATTCAAAG+TGG | - | chr4.4:30625322-30625341 | MS.gene56535:CDS | 35.0% | |
| TTTAAAAGTGGAGTGTGATG+AGG | - | chr4.4:30625393-30625412 | MS.gene56535:CDS | 35.0% | |
| ! | ATTGTTGTGATGGAAAAGCT+GGG | - | chr4.4:30625370-30625389 | MS.gene56535:CDS | 35.0% |
| !! | AAGAGAGTCTTGTGTTGTTT+GGG | - | chr4.4:30625550-30625569 | MS.gene56535:intron | 35.0% |
| !! | CTATGAGTTTGTTGTGTTGA+TGG | - | chr4.4:30625648-30625667 | MS.gene56535:CDS | 35.0% |
| !! | GTGTTGATGGAACAAAGTTT+TGG | - | chr4.4:30625661-30625680 | MS.gene56535:CDS | 35.0% |
| !! | GTTTGAAAATGGGGTTAGTT+TGG | - | chr4.4:30625459-30625478 | MS.gene56535:CDS | 35.0% |
| GCAGTTGATCAAAATGGAGA+TGG | - | chr4.4:30625613-30625632 | MS.gene56535:intron | 40.0% | |
| GGAAAAGCTGGGTTTAAAAG+TGG | - | chr4.4:30625381-30625400 | MS.gene56535:CDS | 40.0% | |
| ! | CATTGTTGTGATGGAAAAGC+TGG | - | chr4.4:30625369-30625388 | MS.gene56535:CDS | 40.0% |
| ! | GAAGAGAGTCTTGTGTTGTT+TGG | - | chr4.4:30625549-30625568 | MS.gene56535:intron | 40.0% |
| ! | GGGATTGCATAGAGATTTTG+TGG | - | chr4.4:30625570-30625589 | MS.gene56535:intron | 40.0% |
| AGTGTGATGAGGATGATCCA+GGG | - | chr4.4:30625404-30625423 | MS.gene56535:CDS | 45.0% | |
| TGTTCATCACCAAATTCCCC+TGG | + | chr4.4:30625424-30625443 | None:intergenic | 45.0% | |
| GAGGATGATCCAGGGGAATT+TGG | - | chr4.4:30625412-30625431 | MS.gene56535:CDS | 50.0% | |
| GAGTGTGATGAGGATGATCC+AGG | - | chr4.4:30625403-30625422 | MS.gene56535:CDS | 50.0% | |
| GTGTGATGAGGATGATCCAG+GGG | - | chr4.4:30625405-30625424 | MS.gene56535:CDS | 50.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr4.4 | gene | 30625136 | 30625687 | 30625136 | ID=MS.gene56535 |
| chr4.4 | mRNA | 30625136 | 30625687 | 30625136 | ID=MS.gene56535.t1;Parent=MS.gene56535 |
| chr4.4 | exon | 30625643 | 30625687 | 30625643 | ID=MS.gene56535.t1.exon1;Parent=MS.gene56535.t1 |
| chr4.4 | CDS | 30625643 | 30625687 | 30625643 | ID=cds.MS.gene56535.t1;Parent=MS.gene56535.t1 |
| chr4.4 | exon | 30625136 | 30625558 | 30625136 | ID=MS.gene56535.t1.exon2;Parent=MS.gene56535.t1 |
| chr4.4 | CDS | 30625136 | 30625558 | 30625136 | ID=cds.MS.gene56535.t1;Parent=MS.gene56535.t1 |
| Gene Sequence |
| Protein sequence |
