Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene57238.t1 | XP_003589004.1 | 79.5 | 229 | 47 | 0 | 1 | 229 | 1 | 229 | 2.80E-57 | 232.3 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene57238.t1 | G7I9Z4 | 79.5 | 229 | 47 | 0 | 1 | 229 | 1 | 229 | 2.0e-57 | 232.3 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene052434 | MS.gene57238 | 0.80816 | 3.55E-50 | -1.69E-46 |
| MS.gene053109 | MS.gene57238 | 0.808961 | 2.39E-50 | -1.69E-46 |
| MS.gene058654 | MS.gene57238 | 0.804614 | 1.98E-49 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene57238.t1 | MTR_1g016270 | 92.029 | 276 | 22 | 0 | 1 | 276 | 1 | 276 | 8.26e-148 | 415 |
| MS.gene57238.t1 | MTR_8g097140 | 47.464 | 276 | 93 | 9 | 4 | 271 | 3 | 234 | 2.22e-51 | 168 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene57238.t1 | AT5G60030 | 59.322 | 59 | 24 | 0 | 1 | 59 | 1 | 59 | 5.15e-17 | 79.0 |
| MS.gene57238.t1 | AT1G75335 | 59.322 | 59 | 24 | 0 | 1 | 59 | 1 | 59 | 9.02e-16 | 71.6 |
Find 64 sgRNAs with CRISPR-Local
Find 70 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| GGAAAGAAGAAACTAGAATT+TGG | 0.282317 | 1.4:-6070171 | MS.gene57238:CDS |
| AAGAAGGAGAAGAGTGTAGA+AGG | 0.312453 | 1.4:-6070057 | MS.gene57238:CDS |
| AAGAAGAATGGAAGTGAGTT+AGG | 0.374907 | 1.4:-6070138 | MS.gene57238:CDS |
| TGAAAAGGAGAAGAATTTAG+AGG | 0.379641 | 1.4:-6069950 | MS.gene57238:CDS |
| GTTGTCAGCAGAGACAAATT+TGG | 0.400587 | 1.4:+6070428 | None:intergenic |
| ATTCTCGGGATGTAGAAATT+AGG | 0.415570 | 1.4:-6069840 | MS.gene57238:CDS |
| AGAAGGAGAAGAGTGTAGAA+GGG | 0.423276 | 1.4:-6070056 | MS.gene57238:CDS |
| AATGGTGTGAAGAGTGAAAA+TGG | 0.435529 | 1.4:-6069874 | MS.gene57238:CDS |
| GAAGACACGGAAGAGAGATC+TGG | 0.440783 | 1.4:-6069733 | MS.gene57238:CDS |
| ATGGAGGGCTAGTTGATTCT+CGG | 0.450550 | 1.4:-6069855 | MS.gene57238:CDS |
| AAGGAGAAGAATTTAGAGGT+TGG | 0.455722 | 1.4:-6069946 | MS.gene57238:CDS |
| TTAAACCGGCGCAGTCTCAT+AGG | 0.455996 | 1.4:-6070326 | MS.gene57238:CDS |
| TGAGCTTAATCAACTTCATA+AGG | 0.457180 | 1.4:-6070352 | MS.gene57238:CDS |
| AGTCTAAGAAGAAAATGAAA+AGG | 0.460042 | 1.4:-6069705 | MS.gene57238:CDS |
| ATGTGAAGGTTGAAAGTAAA+GGG | 0.462456 | 1.4:-6070029 | MS.gene57238:CDS |
| GGAGAGAGTGTAAAGAAGAA+TGG | 0.469789 | 1.4:-6070150 | MS.gene57238:CDS |
| CTCCTCAGGAGTTAGAAATT+AGG | 0.475036 | 1.4:-6069999 | MS.gene57238:CDS |
| GAGAAGCGTGATAAGAAAGA+TGG | 0.475565 | 1.4:-6070192 | MS.gene57238:CDS |
| AAATGAAGTGGGGAGTGAAA+AGG | 0.480460 | 1.4:-6069965 | MS.gene57238:CDS |
| AACAGATGATAGTGGTAGAT+TGG | 0.498404 | 1.4:-6070283 | MS.gene57238:CDS |
| TGGAGGGCTAGTTGATTCTC+GGG | 0.502825 | 1.4:-6069854 | MS.gene57238:CDS |
| AAGACACGGAAGAGAGATCT+GGG | 0.505868 | 1.4:-6069732 | MS.gene57238:CDS |
| ACTTCATAAGGAGCTTAAAC+CGG | 0.507615 | 1.4:-6070340 | MS.gene57238:CDS |
| GAATGGAAGTGAGTTAGGTC+AGG | 0.510402 | 1.4:-6070133 | MS.gene57238:CDS |
| GAAGTTGTTGGTTCTGTCGT+GGG | 0.512214 | 1.4:-6070222 | MS.gene57238:CDS |
| GAAGGAGAAGAGTGTAGAAG+GGG | 0.517307 | 1.4:-6070055 | MS.gene57238:CDS |
| CGAGAAGAAGAACAAGAAGA+AGG | 0.521154 | 1.4:-6070073 | MS.gene57238:CDS |
| TAAAGGGATAGTTCCTCCTC+AGG | 0.521560 | 1.4:-6070013 | MS.gene57238:CDS |
| TTTAGAGGTTGGAAATGTGA+AGG | 0.536582 | 1.4:-6069935 | MS.gene57238:CDS |
| TGAAGTTGTTGGTTCTGTCG+TGG | 0.542751 | 1.4:-6070223 | MS.gene57238:CDS |
| AATGTGAAGGTTGAAAGTAA+AGG | 0.544852 | 1.4:-6070030 | MS.gene57238:CDS |
| AGACGGAGCAAAGGAAGAAG+AGG | 0.546821 | 1.4:-6069762 | MS.gene57238:CDS |
| CATTGAAAGCCGTGGATGCA+CGG | 0.548708 | 1.4:+6070373 | None:intergenic |
| AAGAGGGAAAGACGGAGCAA+AGG | 0.550638 | 1.4:-6069771 | MS.gene57238:CDS |
| AAACCTGGTCGTGAAGTTGT+TGG | 0.551304 | 1.4:-6070234 | MS.gene57238:CDS |
| GAGTTAGGTCAGGACGATGA+AGG | 0.567638 | 1.4:-6070123 | MS.gene57238:CDS |
| GAGTAAATTACATGCTGAAG+AGG | 0.574245 | 1.4:-6069788 | MS.gene57238:CDS |
| GGGACGAGAACAACAGAAAG+AGG | 0.576625 | 1.4:-6069914 | MS.gene57238:CDS |
| TGTAGAAGGGGAGAATGTGA+AGG | 0.579317 | 1.4:-6070043 | MS.gene57238:CDS |
| TTAGAGGTTGGAAATGTGAA+GGG | 0.581388 | 1.4:-6069934 | MS.gene57238:CDS |
| GATTGGTCGAAAGTACTGTT+CGG | 0.581744 | 1.4:-6070266 | MS.gene57238:CDS |
| TACCACTATCATCTGTTGCA+TGG | 0.584555 | 1.4:+6070289 | None:intergenic |
| AGCCATGCAACAGATGATAG+TGG | 0.587057 | 1.4:-6070291 | MS.gene57238:CDS |
| ATTAAGCTCATTGAAAGCCG+TGG | 0.591302 | 1.4:+6070365 | None:intergenic |
| GACGATGAAGGAAGAACGGA+GGG | 0.595325 | 1.4:-6070111 | MS.gene57238:CDS |
| TTATCTGCTCCGTGCATCCA+CGG | 0.595538 | 1.4:-6070382 | MS.gene57238:CDS |
| AGTAAATTACATGCTGAAGA+GGG | 0.598011 | 1.4:-6069787 | MS.gene57238:CDS |
| GACCTAATTTCTAACTCCTG+AGG | 0.598620 | 1.4:+6069997 | None:intergenic |
| GAACCAACAACTTCACGACC+AGG | 0.602335 | 1.4:+6070231 | None:intergenic |
| GTTCGGAGTGATGTTAAACC+TGG | 0.607851 | 1.4:-6070249 | MS.gene57238:CDS |
| GTTGTTGGTTCTGTCGTGGG+TGG | 0.608189 | 1.4:-6070219 | MS.gene57238:CDS |
| ACGAGAACAACAGAAAGAGG+TGG | 0.612657 | 1.4:-6069911 | MS.gene57238:CDS |
| TCAGGACGATGAAGGAAGAA+CGG | 0.621358 | 1.4:-6070115 | MS.gene57238:CDS |
| ACATGCTGAAGAGGGAAAGA+CGG | 0.635975 | 1.4:-6069779 | MS.gene57238:CDS |
| TTCTTCCTATGAGACTGCGC+CGG | 0.636496 | 1.4:+6070321 | None:intergenic |
| GAAGAGGAAGAATGAAGACA+CGG | 0.643138 | 1.4:-6069746 | MS.gene57238:CDS |
| AAATTTGTCTCTGCTGACAA+CGG | 0.644626 | 1.4:-6070426 | MS.gene57238:CDS |
| CTAATTTCTAACTCCTGAGG+AGG | 0.647828 | 1.4:+6070000 | None:intergenic |
| GGACGATGAAGGAAGAACGG+AGG | 0.651421 | 1.4:-6070112 | MS.gene57238:CDS |
| GAAAATGAAAAGGAAACACG+AGG | 0.691720 | 1.4:-6069695 | MS.gene57238:CDS |
| GTGTGAAGAGTGAAAATGGA+GGG | 0.692434 | 1.4:-6069870 | MS.gene57238:CDS |
| AAAATGAAAAGGAAACACGA+GGG | 0.733622 | 1.4:-6069694 | MS.gene57238:CDS |
| AGACACGGAAGAGAGATCTG+GGG | 0.742079 | 1.4:-6069731 | MS.gene57238:CDS |
| GGTGTGAAGAGTGAAAATGG+AGG | 0.808410 | 1.4:-6069871 | MS.gene57238:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| ! | AGTCTAAGAAGAAAATGAAA+AGG | - | chr1.4:6070483-6070502 | MS.gene57238:CDS | 25.0% |
| AAAATGAAAAGGAAACACGA+GGG | - | chr1.4:6070494-6070513 | MS.gene57238:CDS | 30.0% | |
| AATGTGAAGGTTGAAAGTAA+AGG | - | chr1.4:6070158-6070177 | MS.gene57238:CDS | 30.0% | |
| AGTAAATTACATGCTGAAGA+GGG | - | chr1.4:6070401-6070420 | MS.gene57238:CDS | 30.0% | |
| ATGTGAAGGTTGAAAGTAAA+GGG | - | chr1.4:6070159-6070178 | MS.gene57238:CDS | 30.0% | |
| GGAAAGAAGAAACTAGAATT+TGG | - | chr1.4:6070017-6070036 | MS.gene57238:CDS | 30.0% | |
| TCGAAAAAGAGAAATGAAGT+GGG | - | chr1.4:6070212-6070231 | MS.gene57238:CDS | 30.0% | |
| TGAAAAGGAGAAGAATTTAG+AGG | - | chr1.4:6070238-6070257 | MS.gene57238:CDS | 30.0% | |
| TGAGCTTAATCAACTTCATA+AGG | - | chr1.4:6069836-6069855 | MS.gene57238:CDS | 30.0% | |
| AAATTTGTCTCTGCTGACAA+CGG | - | chr1.4:6069762-6069781 | MS.gene57238:CDS | 35.0% | |
| AAGAAGAATGGAAGTGAGTT+AGG | - | chr1.4:6070050-6070069 | MS.gene57238:CDS | 35.0% | |
| AAGGAGAAGAATTTAGAGGT+TGG | - | chr1.4:6070242-6070261 | MS.gene57238:CDS | 35.0% | |
| AATGGTGTGAAGAGTGAAAA+TGG | - | chr1.4:6070314-6070333 | MS.gene57238:CDS | 35.0% | |
| ACTTCATAAGGAGCTTAAAC+CGG | - | chr1.4:6069848-6069867 | MS.gene57238:CDS | 35.0% | |
| ATTCTCGGGATGTAGAAATT+AGG | - | chr1.4:6070348-6070367 | MS.gene57238:CDS | 35.0% | |
| CGAAAAAGAGAAATGAAGTG+GGG | - | chr1.4:6070213-6070232 | MS.gene57238:CDS | 35.0% | |
| GAAAATGAAAAGGAAACACG+AGG | - | chr1.4:6070493-6070512 | MS.gene57238:CDS | 35.0% | |
| GAGTAAATTACATGCTGAAG+AGG | - | chr1.4:6070400-6070419 | MS.gene57238:CDS | 35.0% | |
| GTCGAAAAAGAGAAATGAAG+TGG | - | chr1.4:6070211-6070230 | MS.gene57238:CDS | 35.0% | |
| GTGGAGAAAAAACTAAGCAA+TGG | - | chr1.4:6070296-6070315 | MS.gene57238:CDS | 35.0% | |
| TTAGAGGTTGGAAATGTGAA+GGG | - | chr1.4:6070254-6070273 | MS.gene57238:CDS | 35.0% | |
| TTTAGAGGTTGGAAATGTGA+AGG | - | chr1.4:6070253-6070272 | MS.gene57238:CDS | 35.0% | |
| ! | CGAAAAAGAAGCATGAAACT+GGG | - | chr1.4:6070372-6070391 | MS.gene57238:CDS | 35.0% |
| ! | TCGAAAAAGAAGCATGAAAC+TGG | - | chr1.4:6070371-6070390 | MS.gene57238:CDS | 35.0% |
| !! | AACAGATGATAGTGGTAGAT+TGG | - | chr1.4:6069905-6069924 | MS.gene57238:CDS | 35.0% |
| AAATGAAGTGGGGAGTGAAA+AGG | - | chr1.4:6070223-6070242 | MS.gene57238:CDS | 40.0% | |
| ATTAAGCTCATTGAAAGCCG+TGG | + | chr1.4:6069826-6069845 | None:intergenic | 40.0% | |
| CGAGAAGAAGAACAAGAAGA+AGG | - | chr1.4:6070115-6070134 | MS.gene57238:CDS | 40.0% | |
| CTAATTTCTAACTCCTGAGG+AGG | + | chr1.4:6070191-6070210 | None:intergenic | 40.0% | |
| CTCCTCAGGAGTTAGAAATT+AGG | - | chr1.4:6070189-6070208 | MS.gene57238:CDS | 40.0% | |
| GAAGAGGAAGAATGAAGACA+CGG | - | chr1.4:6070442-6070461 | MS.gene57238:CDS | 40.0% | |
| GACCTAATTTCTAACTCCTG+AGG | + | chr1.4:6070194-6070213 | None:intergenic | 40.0% | |
| GTGTGAAGAGTGAAAATGGA+GGG | - | chr1.4:6070318-6070337 | MS.gene57238:CDS | 40.0% | |
| GTTGTCAGCAGAGACAAATT+TGG | + | chr1.4:6069763-6069782 | None:intergenic | 40.0% | |
| TACCACTATCATCTGTTGCA+TGG | + | chr1.4:6069902-6069921 | None:intergenic | 40.0% | |
| ! | AAGAAGGAGAAGAGTGTAGA+AGG | - | chr1.4:6070131-6070150 | MS.gene57238:CDS | 40.0% |
| ! | AGAAGGAGAAGAGTGTAGAA+GGG | - | chr1.4:6070132-6070151 | MS.gene57238:CDS | 40.0% |
| ! | GAGAAGCGTGATAAGAAAGA+TGG | - | chr1.4:6069996-6070015 | MS.gene57238:CDS | 40.0% |
| ! | GGAGAGAGTGTAAAGAAGAA+TGG | - | chr1.4:6070038-6070057 | MS.gene57238:CDS | 40.0% |
| !! | GATTGGTCGAAAGTACTGTT+CGG | - | chr1.4:6069922-6069941 | MS.gene57238:CDS | 40.0% |
| AAACCTGGTCGTGAAGTTGT+TGG | - | chr1.4:6069954-6069973 | MS.gene57238:CDS | 45.0% | |
| AAGACACGGAAGAGAGATCT+GGG | - | chr1.4:6070456-6070475 | MS.gene57238:CDS | 45.0% | |
| ACATGCTGAAGAGGGAAAGA+CGG | - | chr1.4:6070409-6070428 | MS.gene57238:CDS | 45.0% | |
| ACGAGAACAACAGAAAGAGG+TGG | - | chr1.4:6070277-6070296 | MS.gene57238:CDS | 45.0% | |
| AGCCATGCAACAGATGATAG+TGG | - | chr1.4:6069897-6069916 | MS.gene57238:CDS | 45.0% | |
| GAATGGAAGTGAGTTAGGTC+AGG | - | chr1.4:6070055-6070074 | MS.gene57238:CDS | 45.0% | |
| GGTGTGAAGAGTGAAAATGG+AGG | - | chr1.4:6070317-6070336 | MS.gene57238:CDS | 45.0% | |
| GTTCGGAGTGATGTTAAACC+TGG | - | chr1.4:6069939-6069958 | MS.gene57238:CDS | 45.0% | |
| TAAAGGGATAGTTCCTCCTC+AGG | - | chr1.4:6070175-6070194 | MS.gene57238:CDS | 45.0% | |
| TCAGGACGATGAAGGAAGAA+CGG | - | chr1.4:6070073-6070092 | MS.gene57238:CDS | 45.0% | |
| TGTAGAAGGGGAGAATGTGA+AGG | - | chr1.4:6070145-6070164 | MS.gene57238:CDS | 45.0% | |
| ! | GAAGGAGAAGAGTGTAGAAG+GGG | - | chr1.4:6070133-6070152 | MS.gene57238:CDS | 45.0% |
| !! | ATGGAGGGCTAGTTGATTCT+CGG | - | chr1.4:6070333-6070352 | MS.gene57238:CDS | 45.0% |
| !! | GAAGTTGTTGGTTCTGTCGT+GGG | - | chr1.4:6069966-6069985 | MS.gene57238:CDS | 45.0% |
| !! | TGAAGTTGTTGGTTCTGTCG+TGG | - | chr1.4:6069965-6069984 | MS.gene57238:CDS | 45.0% |
| AAGAGGGAAAGACGGAGCAA+AGG | - | chr1.4:6070417-6070436 | MS.gene57238:CDS | 50.0% | |
| AGACACGGAAGAGAGATCTG+GGG | - | chr1.4:6070457-6070476 | MS.gene57238:CDS | 50.0% | |
| AGACGGAGCAAAGGAAGAAG+AGG | - | chr1.4:6070426-6070445 | MS.gene57238:CDS | 50.0% | |
| CATTGAAAGCCGTGGATGCA+CGG | + | chr1.4:6069818-6069837 | None:intergenic | 50.0% | |
| GAACCAACAACTTCACGACC+AGG | + | chr1.4:6069960-6069979 | None:intergenic | 50.0% | |
| GAAGACACGGAAGAGAGATC+TGG | - | chr1.4:6070455-6070474 | MS.gene57238:CDS | 50.0% | |
| GACGATGAAGGAAGAACGGA+GGG | - | chr1.4:6070077-6070096 | MS.gene57238:CDS | 50.0% | |
| GAGTTAGGTCAGGACGATGA+AGG | - | chr1.4:6070065-6070084 | MS.gene57238:CDS | 50.0% | |
| GGGACGAGAACAACAGAAAG+AGG | - | chr1.4:6070274-6070293 | MS.gene57238:CDS | 50.0% | |
| TTAAACCGGCGCAGTCTCAT+AGG | - | chr1.4:6069862-6069881 | MS.gene57238:CDS | 50.0% | |
| TTATCTGCTCCGTGCATCCA+CGG | - | chr1.4:6069806-6069825 | MS.gene57238:CDS | 50.0% | |
| TTCTTCCTATGAGACTGCGC+CGG | + | chr1.4:6069870-6069889 | None:intergenic | 50.0% | |
| !! | TGGAGGGCTAGTTGATTCTC+GGG | - | chr1.4:6070334-6070353 | MS.gene57238:CDS | 50.0% |
| GGACGATGAAGGAAGAACGG+AGG | - | chr1.4:6070076-6070095 | MS.gene57238:CDS | 55.0% | |
| !! | GTTGTTGGTTCTGTCGTGGG+TGG | - | chr1.4:6069969-6069988 | MS.gene57238:CDS | 55.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr1.4 | gene | 6069690 | 6070520 | 6069690 | ID=MS.gene57238 |
| chr1.4 | mRNA | 6069690 | 6070520 | 6069690 | ID=MS.gene57238.t1;Parent=MS.gene57238 |
| chr1.4 | exon | 6069690 | 6070520 | 6069690 | ID=MS.gene57238.t1.exon1;Parent=MS.gene57238.t1 |
| chr1.4 | CDS | 6069690 | 6070520 | 6069690 | ID=cds.MS.gene57238.t1;Parent=MS.gene57238.t1 |
| Gene Sequence |
| Protein sequence |
