AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene57471

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene57471.t1 MTR_2g035790 91.275 447 28 1 1 436 42 488 0.0 792
MS.gene57471.t1 MTR_4g094732 71.694 431 117 3 9 436 52 480 0.0 617
MS.gene57471.t1 MTR_2g035760 52.542 413 178 7 17 424 82 481 1.49e-140 411
MS.gene57471.t1 MTR_6g015785 52.336 428 180 8 13 436 83 490 1.28e-133 394
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene57471.t1 AT4G20040 64.085 426 146 5 13 436 63 483 0.0 551
MS.gene57471.t1 AT4G20050 54.353 425 170 6 17 436 74 479 4.76e-150 435
MS.gene57471.t1 AT4G20050 54.353 425 170 6 17 436 74 479 4.76e-150 435
MS.gene57471.t1 AT4G20050 54.353 425 170 6 17 436 74 479 4.76e-150 435
MS.gene57471.t1 AT4G20050 54.353 425 170 6 17 436 74 479 4.76e-150 435
MS.gene57471.t1 AT4G20050 54.353 425 170 6 17 436 99 504 5.41e-150 436
MS.gene57471.t1 AT4G20050 53.750 400 161 6 42 436 1 381 7.48e-138 400
MS.gene57471.t1 AT4G20050 53.750 400 161 6 42 436 1 381 7.48e-138 400

Find 85 sgRNAs with CRISPR-Local

Find 200 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
GCATTTGATATTGATAATTT+AGG 0.107857 2.2:-50783836 MS.gene57471:CDS
ACAATTGGAACATTATTATT+AGG 0.129299 2.2:+50781217 None:intergenic
TCAATTAAAGGGAAAGTTTA+TGG 0.147921 2.2:-50781274 MS.gene57471:CDS
GGATCTGCACCATACTTAAT+TGG 0.153680 2.2:+50783908 None:intergenic
CATTTGATATTGATAATTTA+GGG 0.251344 2.2:-50783835 MS.gene57471:CDS
GTTCCAATTGTGAAATTAGA+TGG 0.269638 2.2:-50781205 MS.gene57471:CDS
CATCAATGACCAAAGGTAAA+TGG 0.278889 2.2:-50781946 MS.gene57471:intron
CAATTGGAACATTATTATTA+GGG 0.285274 2.2:+50781218 None:intergenic
TATAACAAAGCAACAGGTTT+TGG 0.286379 2.2:-50781448 MS.gene57471:CDS
ATTGTTAACAATATGTTTAG+TGG 0.301676 2.2:-50781244 MS.gene57471:CDS
TGAAAATGACTAATCCTATT+AGG 0.302520 2.2:+50781043 None:intergenic
CAGCCATAGGAATTGTTGTT+AGG 0.306110 2.2:-50781504 MS.gene57471:CDS
TGGATGGTAATGGCACAAAA+TGG 0.367127 2.2:-50781096 MS.gene57471:CDS
AAATGTTTCAGAAGCTCTTA+AGG 0.373297 2.2:+50781918 None:intergenic
TACTTGATATGAAGGTTTCA+TGG 0.388165 2.2:+50781644 None:intergenic
CCTCCAAGGTCTCTAATTCC+TGG 0.397675 2.2:+50783800 None:intergenic
CATTGTTATAACAAAGCAAC+AGG 0.404226 2.2:-50781454 MS.gene57471:CDS
TAATTCCTTCGGTGGTGAAA+TGG 0.411730 2.2:+50781680 None:intergenic
ACTTTGAACTTTAATTCCTT+CGG 0.420815 2.2:+50781669 None:intergenic
AGCTTCTACAGCTTTCATAA+TGG 0.424025 2.2:+50783859 None:intergenic
TTCGTAACATGAACTTGAAC+AGG 0.426663 2.2:+50781334 None:intergenic
TTGTTCAAGTAATCTGTTGT+TGG 0.430783 2.2:+50781825 None:intergenic
AATTCATTGTTCAACAAAGA+AGG 0.431005 2.2:+50780910 None:intergenic
ACTGAATGTGCAACAAACTT+TGG 0.434126 2.2:+50780995 None:intergenic
AAGAAAACTACTTGATATGA+AGG 0.434177 2.2:+50781636 None:intergenic
AAGTATGGTGCAGATCCAAC+AGG 0.439437 2.2:-50783902 MS.gene57471:CDS
ATATTCATTTCATGCATTAG+AGG 0.439787 2.2:-50781020 MS.gene57471:CDS
GATCTGCACCATACTTAATT+GGG 0.441924 2.2:+50783909 None:intergenic
ATCACATTTCCTTCATCGGC+TGG 0.454793 2.2:-50783731 MS.gene57471:CDS
AGATTACTTGAACAAACAAT+AGG 0.463517 2.2:-50781817 MS.gene57471:CDS
TCCCATCACATTTCCTTCAT+CGG 0.471050 2.2:-50783735 MS.gene57471:CDS
AGCCATAGGAATTGTTGTTA+GGG 0.472547 2.2:-50781503 MS.gene57471:CDS
GAAAGTGAGAAACCAGTTCA+TGG 0.473042 2.2:-50780941 MS.gene57471:CDS
TTCCAATTGTGAAATTAGAT+GGG 0.473930 2.2:-50781204 MS.gene57471:CDS
AAGGAAACAACCCCATGAAC+TGG 0.475950 2.2:+50780929 None:intergenic
TTACCAGGAATTAGAGACCT+TGG 0.494991 2.2:-50783803 MS.gene57471:CDS
TCCCCTAACAACAATTCCTA+TGG 0.501817 2.2:+50781501 None:intergenic
TATTCATTTCATGCATTAGA+GGG 0.507592 2.2:-50781019 MS.gene57471:CDS
AAGAATTGATAATTGTTACA+TGG 0.510868 2.2:-50781383 MS.gene57471:CDS
GTCAAATAATGTTGTAGTTG+TGG 0.512201 2.2:-50780963 MS.gene57471:CDS
TTAGGGCTTGAATTATTACC+AGG 0.514044 2.2:-50783818 MS.gene57471:CDS
TTCCTCCATTTCACCACCGA+AGG 0.521417 2.2:-50781685 MS.gene57471:CDS
TCAACAAGGTGTCTATCAGT+TGG 0.524915 2.2:+50781895 None:intergenic
GTGGTGTACCCAATTAAGTA+TGG 0.526549 2.2:-50783917 MS.gene57471:CDS
ACAAGATTGCCAGCCGATGA+AGG 0.526613 2.2:+50783722 None:intergenic
CTTGGACAACACTCAACAGT+CGG 0.527472 2.2:-50781616 MS.gene57471:CDS
GTTCAAGTTCATGTTACGAA+TGG 0.528355 2.2:-50781331 MS.gene57471:CDS
GATTCAAGTTACCGAGGTGG+AGG 0.539152 2.2:-50781754 MS.gene57471:CDS
TTGAACTTTAATTCCTTCGG+TGG 0.542054 2.2:+50781672 None:intergenic
ATTGATATGAACAATGTGAT+TGG 0.543536 2.2:-50781154 MS.gene57471:CDS
TGGTAATGGCACAAAATGGG+TGG 0.549729 2.2:-50781092 MS.gene57471:CDS
GGATGGTAATGGCACAAAAT+GGG 0.551911 2.2:-50781095 MS.gene57471:CDS
GGATTATAATTCTATAGTCA+TGG 0.563335 2.2:-50781362 MS.gene57471:CDS
AAAGTGAGAAACCAGTTCAT+GGG 0.566325 2.2:-50780940 MS.gene57471:CDS
TTCATCGGCTGGCAATCTTG+TGG 0.566505 2.2:-50783720 MS.gene57471:intron
TCAACAGTCGGCGGAGACAA+AGG 0.569193 2.2:-50781604 MS.gene57471:CDS
AATGAAGAAGAGTGGAAGAG+TGG 0.571785 2.2:-50783936 MS.gene57471:intron
TTCCTTCGGTGGTGAAATGG+AGG 0.577526 2.2:+50781683 None:intergenic
GCCGATGAAGGAAATGTGAT+GGG 0.577733 2.2:+50783734 None:intergenic
CCAGGAATTAGAGACCTTGG+AGG 0.587401 2.2:-50783800 MS.gene57471:CDS
GGACAAGCTAACATGATCAC+AGG 0.590351 2.2:-50781481 MS.gene57471:CDS
ATTTAGTGACATTGATCAAG+TGG 0.593403 2.2:-50781179 MS.gene57471:CDS
TCCAATTGTGAAATTAGATG+GGG 0.603106 2.2:-50781203 MS.gene57471:CDS
CTCGGTAACTTGAATCGAAG+AGG 0.611266 2.2:+50781761 None:intergenic
AGCCGATGAAGGAAATGTGA+TGG 0.612995 2.2:+50783733 None:intergenic
AAGTTGAATGTGGATGGTAA+TGG 0.619073 2.2:-50781106 MS.gene57471:CDS
AATCAATGATAACACCTCCA+AGG 0.627415 2.2:+50783786 None:intergenic
AACCGTTGGGAAGTTGAATG+TGG 0.628722 2.2:-50781116 MS.gene57471:CDS
TGCTGAGAAAATTGCAACAT+CGG 0.630023 2.2:+50781525 None:intergenic
GTGTGAAGATGGCTGAGACA+TGG 0.634929 2.2:+50784231 None:intergenic
TTCGATTCAAGTTACCGAGG+TGG 0.636613 2.2:-50781757 MS.gene57471:CDS
GCCATAGGAATTGTTGTTAG+GGG 0.637301 2.2:-50781502 MS.gene57471:CDS
CCAATTGTGAAATTAGATGG+GGG 0.638303 2.2:-50781202 MS.gene57471:CDS
GGTAATGGCACAAAATGGGT+GGG 0.641219 2.2:-50781091 MS.gene57471:CDS
AGTATGGTGCAGATCCAACA+GGG 0.649273 2.2:-50783901 MS.gene57471:CDS
ATCCACATTCAACTTCCCAA+CGG 0.649744 2.2:+50781114 None:intergenic
TTGATGCATTTAATTCAACA+AGG 0.650431 2.2:+50781881 None:intergenic
AAGTGAGAAACCAGTTCATG+GGG 0.654495 2.2:-50780939 MS.gene57471:CDS
GTTGGGAAGTTGAATGTGGA+TGG 0.656072 2.2:-50781112 MS.gene57471:CDS
GAAGGAATTAAAGTTCAAAG+TGG 0.662811 2.2:-50781667 MS.gene57471:CDS
CAATGACCAAAGGTAAATGG+AGG 0.667241 2.2:-50781943 MS.gene57471:intron
CTCTTCGATTCAAGTTACCG+AGG 0.668555 2.2:-50781760 MS.gene57471:CDS
GGACAACACTCAACAGTCGG+CGG 0.701448 2.2:-50781613 MS.gene57471:CDS
CGATGAATATTCCTCCACCT+CGG 0.718566 2.2:+50781743 None:intergenic
GTATGGTGCAGATCCAACAG+GGG 0.763386 2.2:-50783900 MS.gene57471:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! AGAGAAAAATATTTATTTAT+AGG + chr2.2:50782047-50782066 None:intergenic 10.0%
!! CTAATTTATATAACAAATTA+AGG + chr2.2:50781559-50781578 None:intergenic 10.0%
!! TCAAAATAATAAATTTGTAT+TGG - chr2.2:50782669-50782688 MS.gene57471:intron 10.0%
!!! ATGAGATTTTAAAAAAATTA+TGG - chr2.2:50782343-50782362 MS.gene57471:intron 10.0%
!!! TTTTGATAATATTTATTAAG+AGG - chr2.2:50781949-50781968 MS.gene57471:intron 10.0%
!!! TTTTTTTTGATGATTTTAAT+TGG - chr2.2:50781009-50781028 MS.gene57471:CDS 10.0%
!! AAATAAAACATTATTTGGAA+TGG + chr2.2:50781915-50781934 None:intergenic 15.0%
!! AAATCATAACATAACAATAT+TGG - chr2.2:50783083-50783102 MS.gene57471:intron 15.0%
!! AATAATATGTAAAAGTTAAG+TGG + chr2.2:50783167-50783186 None:intergenic 15.0%
!! TGTGAATTTAATGATAAATA+AGG + chr2.2:50782886-50782905 None:intergenic 15.0%
!!! AATAAAACATTATTTGGAAT+GGG + chr2.2:50781914-50781933 None:intergenic 15.0%
!!! ATTGTTTTGAAATCAATTAA+AGG - chr2.2:50783868-50783887 MS.gene57471:CDS 15.0%
!!! CATTTGATATTGATAATTTA+GGG - chr2.2:50781319-50781338 MS.gene57471:CDS 15.0%
!!! CATTTTATACAATGAAAAAA+AGG + chr2.2:50781155-50781174 None:intergenic 15.0%
!!! GGAATTTTTTTTTTTGAATA+AGG - chr2.2:50782534-50782553 MS.gene57471:intron 15.0%
!!! TCAAATTTAATTTGTAAACA+TGG - chr2.2:50782459-50782478 MS.gene57471:intron 15.0%
!!! TCTCTTTTGATTTTTTTTAT+TGG - chr2.2:50782062-50782081 MS.gene57471:intron 15.0%
!!! TTGTTTTGAAATCAATTAAA+GGG - chr2.2:50783869-50783888 MS.gene57471:CDS 15.0%
!! ACAATTGGAACATTATTATT+AGG + chr2.2:50783940-50783959 None:intergenic 20.0%
!! ACATCTACTTTAATAGTTTA+TGG - chr2.2:50782924-50782943 MS.gene57471:intron 20.0%
!! ATCATCAAAAAAAAATTCCT+AGG + chr2.2:50781003-50781022 None:intergenic 20.0%
!! ATTGTTAACAATATGTTTAG+TGG - chr2.2:50783910-50783929 MS.gene57471:CDS 20.0%
!! CAATTGGAACATTATTATTA+GGG + chr2.2:50783939-50783958 None:intergenic 20.0%
!! TCGATTCAATAGAATAAAAA+AGG - chr2.2:50782301-50782320 MS.gene57471:intron 20.0%
!!! AAGAATTGATAATTGTTACA+TGG - chr2.2:50783771-50783790 MS.gene57471:CDS 20.0%
!!! AGTTGCAAAATTTTATTGAA+AGG + chr2.2:50782743-50782762 None:intergenic 20.0%
!!! ATAAAACATTATTTGGAATG+GGG + chr2.2:50781913-50781932 None:intergenic 20.0%
!!! GCATTTGATATTGATAATTT+AGG - chr2.2:50781318-50781337 MS.gene57471:CDS 20.0%
!!! TTAACTCTTAACTTGTTTTT+TGG + chr2.2:50781752-50781771 None:intergenic 20.0%
! AAAAAAAATTCCTAGGAAGA+AGG + chr2.2:50780996-50781015 None:intergenic 25.0%
! AAACATCAAAAGAAAAGGAA+TGG - chr2.2:50783030-50783049 MS.gene57471:intron 25.0%
! AAACATCAAAAGAAAAGGAA+TGG + chr2.2:50783049-50783030 None:intergenic 25.0%
! AAGAAAACTACTTGATATGA+AGG + chr2.2:50783521-50783540 None:intergenic 25.0%
! AAGAGTTAAAACAAAGAAGA+GGG - chr2.2:50781763-50781782 MS.gene57471:CDS 25.0%
! ACTTTGAACTTTAATTCCTT+CGG + chr2.2:50783488-50783507 None:intergenic 25.0%
! AGATTACTTGAACAAACAAT+AGG - chr2.2:50783337-50783356 MS.gene57471:intron 25.0%
! AGCTAAAACATCAAAAGAAA+AGG + chr2.2:50782799-50782818 None:intergenic 25.0%
! ATATTCATTTCATGCATTAG+AGG - chr2.2:50784134-50784153 MS.gene57471:intron 25.0%
! ATTGATATGAACAATGTGAT+TGG - chr2.2:50784000-50784019 MS.gene57471:intron 25.0%
! CATAACATAACAATATTGGA+GGG - chr2.2:50783087-50783106 MS.gene57471:intron 25.0%
! GGATTATAATTCTATAGTCA+TGG - chr2.2:50783792-50783811 MS.gene57471:CDS 25.0%
! TAAGAGTTAAAACAAAGAAG+AGG - chr2.2:50781762-50781781 MS.gene57471:CDS 25.0%
! TATTCATTTCATGCATTAGA+GGG - chr2.2:50784135-50784154 MS.gene57471:intron 25.0%
! TCAATTAAAGGGAAAGTTTA+TGG - chr2.2:50783880-50783899 MS.gene57471:CDS 25.0%
! TCATAACATAACAATATTGG+AGG - chr2.2:50783086-50783105 MS.gene57471:intron 25.0%
! TGAAAATGACTAATCCTATT+AGG + chr2.2:50784114-50784133 None:intergenic 25.0%
! TTCCAATTGTGAAATTAGAT+GGG - chr2.2:50783950-50783969 MS.gene57471:intron 25.0%
! TTCTATGATGTACATATGTT+AGG - chr2.2:50782642-50782661 MS.gene57471:intron 25.0%
! TTGATGCATTTAATTCAACA+AGG + chr2.2:50783276-50783295 None:intergenic 25.0%
!! ATATTATTTTCCTTCTTCCT+AGG - chr2.2:50780983-50781002 MS.gene57471:CDS 25.0%
!! CACTAAAACATCAAAAGTTT+TGG - chr2.2:50782989-50783008 MS.gene57471:intron 25.0%
!! CATTTTTCTAACATTGTTTG+AGG - chr2.2:50781080-50781099 MS.gene57471:CDS 25.0%
!! TATTCAATGCAAGAACATTT+TGG + chr2.2:50782090-50782109 None:intergenic 25.0%
!! TTCATATCAAGTAGTTTTCT+TGG - chr2.2:50783520-50783539 MS.gene57471:intron 25.0%
!! TTTTCTTGAATGATTCTTGA+AGG + chr2.2:50781885-50781904 None:intergenic 25.0%
!!! ATCAGTAGATATTTTTGAAG+AGG - chr2.2:50782513-50782532 MS.gene57471:intron 25.0%
!!! ATGTTTTGGTATTTCCTAAT+AGG - chr2.2:50784097-50784116 MS.gene57471:intron 25.0%
!!! CTCTAAAACATCAAAAGAAA+AGG - chr2.2:50783025-50783044 MS.gene57471:intron 25.0%
!!! TTACGAATGGTTTTTTCTTA+GGG - chr2.2:50783836-50783855 MS.gene57471:CDS 25.0%
AAATGTTTCAGAAGCTCTTA+AGG + chr2.2:50783239-50783258 None:intergenic 30.0%
ATCAAAAGAAAAGGAATGGA+GGG - chr2.2:50783034-50783053 MS.gene57471:intron 30.0%
ATCAAAAGAAAAGGAATGGA+TGG + chr2.2:50783053-50783034 None:intergenic 30.0%
ATCAATCAAAGTAGTAAAGG+AGG + chr2.2:50781495-50781514 None:intergenic 30.0%
ATTAAAGTAGATGTGACCTT+TGG + chr2.2:50782918-50782937 None:intergenic 30.0%
ATTCACATGTGATAAACCAA+AGG - chr2.2:50782899-50782918 MS.gene57471:intron 30.0%
ATTTAGTGACATTGATCAAG+TGG - chr2.2:50783975-50783994 MS.gene57471:intron 30.0%
CATTGTTATAACAAAGCAAC+AGG - chr2.2:50783700-50783719 MS.gene57471:intron 30.0%
GAAGGAATTAAAGTTCAAAG+TGG - chr2.2:50783487-50783506 MS.gene57471:intron 30.0%
GTCAAATAATGTTGTAGTTG+TGG - chr2.2:50784191-50784210 MS.gene57471:intron 30.0%
GTTCCAATTGTGAAATTAGA+TGG - chr2.2:50783949-50783968 MS.gene57471:intron 30.0%
TACTTGATATGAAGGTTTCA+TGG + chr2.2:50783513-50783532 None:intergenic 30.0%
TCCAATTGTGAAATTAGATG+GGG - chr2.2:50783951-50783970 MS.gene57471:intron 30.0%
TCTCACCAAACAATACTAAA+GGG - chr2.2:50782217-50782236 MS.gene57471:intron 30.0%
TGCATCAATCAAAGTAGTAA+AGG + chr2.2:50781498-50781517 None:intergenic 30.0%
TGGAGTTATTAAGAAATTGC+AGG + chr2.2:50782155-50782174 None:intergenic 30.0%
TTGAGTGAGACTTAAATTGA+TGG + chr2.2:50782175-50782194 None:intergenic 30.0%
TTGTTCAAGTAATCTGTTGT+TGG + chr2.2:50783332-50783351 None:intergenic 30.0%
! AAAGGTGAAAGACAATTTTC+GGG - chr2.2:50783568-50783587 MS.gene57471:intron 30.0%
! ACTTTTACATCAATGACCAA+AGG - chr2.2:50783201-50783220 MS.gene57471:intron 30.0%
! AGAACTCGAAAAAAGTACAA+GGG - chr2.2:50781714-50781733 MS.gene57471:CDS 30.0%
! CAAAGGTGAAAGACAATTTT+CGG - chr2.2:50783567-50783586 MS.gene57471:intron 30.0%
! GAATTTTGTTGAAGTTAGCA+GGG - chr2.2:50783737-50783756 MS.gene57471:CDS 30.0%
! TATAACAAAGCAACAGGTTT+TGG - chr2.2:50783706-50783725 MS.gene57471:intron 30.0%
! TTTATGGTGTTTTCCAACAA+GGG - chr2.2:50782940-50782959 MS.gene57471:intron 30.0%
!! GATTTGTTCATTTTCCAACA+AGG + chr2.2:50782279-50782298 None:intergenic 30.0%
!! GGGTGATTTTTCAAATGTTT+TGG - chr2.2:50784083-50784102 MS.gene57471:intron 30.0%
!!! ACCTTTTTTTTGTGTGAAGA+TGG + chr2.2:50780937-50780956 None:intergenic 30.0%
!!! ACTCTTAACTTGTTTTTTGG+TGG + chr2.2:50781749-50781768 None:intergenic 30.0%
!!! ATGATTTTAAGATCAACCGT+TGG - chr2.2:50784024-50784043 MS.gene57471:intron 30.0%
!!! CTCTTAACTTGTTTTTTGGT+GGG + chr2.2:50781748-50781767 None:intergenic 30.0%
!!! GGTGTTATCATTGATTTTCA+AGG - chr2.2:50781375-50781394 MS.gene57471:CDS 30.0%
!!! GTTACGAATGGTTTTTTCTT+AGG - chr2.2:50783835-50783854 MS.gene57471:CDS 30.0%
!!! GTTATCATTGATTTTCAAGG+TGG - chr2.2:50781378-50781397 MS.gene57471:CDS 30.0%
!!! TACGAATGGTTTTTTCTTAG+GGG - chr2.2:50783837-50783856 MS.gene57471:CDS 30.0%
!!! TCTTTTGATGTTTTAGCTCT+TGG - chr2.2:50782801-50782820 MS.gene57471:intron 30.0%
!!! TGATTTTAAGATCAACCGTT+GGG - chr2.2:50784025-50784044 MS.gene57471:intron 30.0%
AAAGTGAGAAACCAGTTCAT+GGG - chr2.2:50784214-50784233 MS.gene57471:intron 35.0%
AAGTTGAATGTGGATGGTAA+TGG - chr2.2:50784048-50784067 MS.gene57471:intron 35.0%
AATCAATGATAACACCTCCA+AGG + chr2.2:50781371-50781390 None:intergenic 35.0%
ACAATCTCATTCTCTCTATG+TGG - chr2.2:50781182-50781201 MS.gene57471:CDS 35.0%
ACTGAATGTGCAACAAACTT+TGG + chr2.2:50784162-50784181 None:intergenic 35.0%
AGCTTCTACAGCTTTCATAA+TGG + chr2.2:50781298-50781317 None:intergenic 35.0%
CAAAGTAGTAAAGGAGGAAA+AGG + chr2.2:50781489-50781508 None:intergenic 35.0%
CATCAAAAGAAAAGGAATGG+AGG - chr2.2:50783033-50783052 MS.gene57471:intron 35.0%
CATCAATGACCAAAGGTAAA+TGG - chr2.2:50783208-50783227 MS.gene57471:intron 35.0%
CCAATTGTGAAATTAGATGG+GGG - chr2.2:50783952-50783971 MS.gene57471:intron 35.0%
CTCACCAAACAATACTAAAG+GGG - chr2.2:50782218-50782237 MS.gene57471:intron 35.0%
CTCTCACCAAACAATACTAA+AGG - chr2.2:50782216-50782235 MS.gene57471:intron 35.0%
GATCTGCACCATACTTAATT+GGG + chr2.2:50781248-50781267 None:intergenic 35.0%
GATTGGAAAATGAAGAAGAG+TGG - chr2.2:50781210-50781229 MS.gene57471:CDS 35.0%
GCACAAACCAGACAAAATAA+AGG - chr2.2:50782413-50782432 MS.gene57471:intron 35.0%
GCCATCTTCACACAAAAAAA+AGG - chr2.2:50780933-50780952 MS.gene57471:CDS 35.0%
GGATGGAGTAAGATGTTAAA+AGG + chr2.2:50782773-50782792 None:intergenic 35.0%
TCACCAAACAATACTAAAGG+GGG - chr2.2:50782219-50782238 MS.gene57471:intron 35.0%
TGCTGAGAAAATTGCAACAT+CGG + chr2.2:50783632-50783651 None:intergenic 35.0%
TTAGGGCTTGAATTATTACC+AGG - chr2.2:50781336-50781355 MS.gene57471:CDS 35.0%
TTCCAGCTACGAAAAAATTG+AGG + chr2.2:50781818-50781837 None:intergenic 35.0%
TTCGTAACATGAACTTGAAC+AGG + chr2.2:50783823-50783842 None:intergenic 35.0%
TTGAACTTTAATTCCTTCGG+TGG + chr2.2:50783485-50783504 None:intergenic 35.0%
TTTCTCAAGTTTACCCTTGT+TGG + chr2.2:50782956-50782975 None:intergenic 35.0%
! AGCCATAGGAATTGTTGTTA+GGG - chr2.2:50783651-50783670 MS.gene57471:intron 35.0%
! AGTGGGTTCAAAATTGTTAG+AGG + chr2.2:50782198-50782217 None:intergenic 35.0%
! ATGCTTTTGTTGCATTTTGG+AGG + chr2.2:50783309-50783328 None:intergenic 35.0%
! CAGAACTCGAAAAAAGTACA+AGG - chr2.2:50781713-50781732 MS.gene57471:CDS 35.0%
! GGAATTTTGTTGAAGTTAGC+AGG - chr2.2:50783736-50783755 MS.gene57471:CDS 35.0%
! GTTCAAGTTCATGTTACGAA+TGG - chr2.2:50783823-50783842 MS.gene57471:CDS 35.0%
! GTTTATGGTGTTTTCCAACA+AGG - chr2.2:50782939-50782958 MS.gene57471:intron 35.0%
! TAGTATTGTTTGGTGAGAGT+GGG + chr2.2:50782215-50782234 None:intergenic 35.0%
! TCATTCTCTCTATGTGGTTT+TGG - chr2.2:50781188-50781207 MS.gene57471:CDS 35.0%
! TGCTTTTGTTGCATTTTGGA+GGG + chr2.2:50783308-50783327 None:intergenic 35.0%
! TGGATGCTTTTGTTGCATTT+TGG + chr2.2:50783312-50783331 None:intergenic 35.0%
! TTAGTATTGTTTGGTGAGAG+TGG + chr2.2:50782216-50782235 None:intergenic 35.0%
! TTATCTGTTTTGCACTGCTA+AGG + chr2.2:50782583-50782602 None:intergenic 35.0%
! TTCATTTTCCAACAAGGCTT+TGG + chr2.2:50782273-50782292 None:intergenic 35.0%
!!! AACCTCAATTTTTTCGTAGC+TGG - chr2.2:50781813-50781832 MS.gene57471:CDS 35.0%
!!! AATGGTTTTTTCTTAGGGGA+TGG - chr2.2:50783841-50783860 MS.gene57471:CDS 35.0%
!!! CAGATCTCCTTTATTTTGTC+TGG + chr2.2:50782423-50782442 None:intergenic 35.0%
AAGGTTCCTCCATTTACCTT+TGG + chr2.2:50783220-50783239 None:intergenic 40.0%
AAGTGAGAAACCAGTTCATG+GGG - chr2.2:50784215-50784234 MS.gene57471:intron 40.0%
AATGAAGAAGAGTGGAAGAG+TGG - chr2.2:50781218-50781237 MS.gene57471:CDS 40.0%
ATCCACATTCAACTTCCCAA+CGG + chr2.2:50784043-50784062 None:intergenic 40.0%
CAATGACCAAAGGTAAATGG+AGG - chr2.2:50783211-50783230 MS.gene57471:intron 40.0%
CCCCCATCTAATTTCACAAT+TGG + chr2.2:50783955-50783974 None:intergenic 40.0%
GAAAGTGAGAAACCAGTTCA+TGG - chr2.2:50784213-50784232 MS.gene57471:intron 40.0%
GGATCTGCACCATACTTAAT+TGG + chr2.2:50781249-50781268 None:intergenic 40.0%
GTGGTGTACCCAATTAAGTA+TGG - chr2.2:50781237-50781256 MS.gene57471:CDS 40.0%
TAATTCCTTCGGTGGTGAAA+TGG + chr2.2:50783477-50783496 None:intergenic 40.0%
TAGCCCCCTTTAGTATTGTT+TGG + chr2.2:50782225-50782244 None:intergenic 40.0%
TCCCATCACATTTCCTTCAT+CGG - chr2.2:50781419-50781438 MS.gene57471:CDS 40.0%
TCCCCTAACAACAATTCCTA+TGG + chr2.2:50783656-50783675 None:intergenic 40.0%
TTACCAGGAATTAGAGACCT+TGG - chr2.2:50781351-50781370 MS.gene57471:CDS 40.0%
! AGCTCTTGGCACAAGTTTTA+AGG - chr2.2:50782815-50782834 MS.gene57471:intron 40.0%
! CAGCCATAGGAATTGTTGTT+AGG - chr2.2:50783650-50783669 MS.gene57471:intron 40.0%
! GCCATAGGAATTGTTGTTAG+GGG - chr2.2:50783652-50783671 MS.gene57471:intron 40.0%
! TCAACAAGGTGTCTATCAGT+TGG + chr2.2:50783262-50783281 None:intergenic 40.0%
! TGAGAAATCCAAAGCCTTGT+TGG - chr2.2:50782262-50782281 MS.gene57471:intron 40.0%
!! AACAAAGCAACAGGTTTTGG+TGG - chr2.2:50783709-50783728 MS.gene57471:intron 40.0%
!! ACAAGGCTTTGGATTTCTCA+AGG + chr2.2:50782262-50782281 None:intergenic 40.0%
!! ACCTCGAAGAGGTTGATATA+CGG - chr2.2:50781841-50781860 MS.gene57471:CDS 40.0%
!! GGATGGTAATGGCACAAAAT+GGG - chr2.2:50784059-50784078 MS.gene57471:intron 40.0%
!! TGGATGGTAATGGCACAAAA+TGG - chr2.2:50784058-50784077 MS.gene57471:intron 40.0%
!! TGTTGCATTTTGGAGGGTTT+TGG + chr2.2:50783302-50783321 None:intergenic 40.0%
!!! CTCTCTATGTGGTTTTGGAT+TGG - chr2.2:50781193-50781212 MS.gene57471:CDS 40.0%
AACCGTTGGGAAGTTGAATG+TGG - chr2.2:50784038-50784057 MS.gene57471:intron 45.0%
AAGGAAACAACCCCATGAAC+TGG + chr2.2:50784228-50784247 None:intergenic 45.0%
AAGTATGGTGCAGATCCAAC+AGG - chr2.2:50781252-50781271 MS.gene57471:CDS 45.0%
AGCCGATGAAGGAAATGTGA+TGG + chr2.2:50781424-50781443 None:intergenic 45.0%
AGTATGGTGCAGATCCAACA+GGG - chr2.2:50781253-50781272 MS.gene57471:CDS 45.0%
ATCACATTTCCTTCATCGGC+TGG - chr2.2:50781423-50781442 MS.gene57471:CDS 45.0%
CGATGAATATTCCTCCACCT+CGG + chr2.2:50783414-50783433 None:intergenic 45.0%
CTCGGTAACTTGAATCGAAG+AGG + chr2.2:50783396-50783415 None:intergenic 45.0%
CTTGGACAACACTCAACAGT+CGG - chr2.2:50783538-50783557 MS.gene57471:intron 45.0%
GCCGATGAAGGAAATGTGAT+GGG + chr2.2:50781423-50781442 None:intergenic 45.0%
GGACAAGCTAACATGATCAC+AGG - chr2.2:50783673-50783692 MS.gene57471:intron 45.0%
GTGTTTCCTAGTACGCTTCT+AGG + chr2.2:50782129-50782148 None:intergenic 45.0%
GTTGGGAAGTTGAATGTGGA+TGG - chr2.2:50784042-50784061 MS.gene57471:intron 45.0%
TCCGTATATCAACCTCTTCG+AGG + chr2.2:50781845-50781864 None:intergenic 45.0%
! CTCTTCGATTCAAGTTACCG+AGG - chr2.2:50783394-50783413 MS.gene57471:intron 45.0%
! CTGCTTTCATTTTCCCCTGT+TGG + chr2.2:50781270-50781289 None:intergenic 45.0%
! GCAATTTTCTCAGCAGCCAT+AGG - chr2.2:50783637-50783656 MS.gene57471:intron 45.0%
! GGTAATGGCACAAAATGGGT+GGG - chr2.2:50784063-50784082 MS.gene57471:intron 45.0%
! TTCGATTCAAGTTACCGAGG+TGG - chr2.2:50783397-50783416 MS.gene57471:intron 45.0%
!! GCAACAGGTTTTGGTGGAAT+TGG - chr2.2:50783715-50783734 MS.gene57471:intron 45.0%
!! TGGTAATGGCACAAAATGGG+TGG - chr2.2:50784062-50784081 MS.gene57471:intron 45.0%
!! AATTTAAATAAAACATTATT+TGG + chr2.2:50781920-50781939 None:intergenic 5.0%
ACAAGATTGCCAGCCGATGA+AGG + chr2.2:50781435-50781454 None:intergenic 50.0%
AGCTGGAAAACACCTCGAAG+AGG - chr2.2:50781830-50781849 MS.gene57471:CDS 50.0%
CCAGGAATTAGAGACCTTGG+AGG - chr2.2:50781354-50781373 MS.gene57471:CDS 50.0%
CCTAGTACGCTTCTAGGTAC+AGG + chr2.2:50782123-50782142 None:intergenic 50.0%
CCTCCAAGGTCTCTAATTCC+TGG + chr2.2:50781357-50781376 None:intergenic 50.0%
GTATGGTGCAGATCCAACAG+GGG - chr2.2:50781254-50781273 MS.gene57471:CDS 50.0%
GTGTGAAGATGGCTGAGACA+TGG + chr2.2:50780926-50780945 None:intergenic 50.0%
TTCCTCCATTTCACCACCGA+AGG - chr2.2:50783469-50783488 MS.gene57471:intron 50.0%
TTCCTTCGGTGGTGAAATGG+AGG + chr2.2:50783474-50783493 None:intergenic 50.0%
! CCTGTACCTAGAAGCGTACT+AGG - chr2.2:50782120-50782139 MS.gene57471:intron 50.0%
! GATTCAAGTTACCGAGGTGG+AGG - chr2.2:50783400-50783419 MS.gene57471:intron 50.0%
! TTCATCGGCTGGCAATCTTG+TGG - chr2.2:50781434-50781453 MS.gene57471:CDS 50.0%
GCTTCTAGGTACAGGCTCAC+TGG + chr2.2:50782115-50782134 None:intergenic 55.0%
GGACAACACTCAACAGTCGG+CGG - chr2.2:50783541-50783560 MS.gene57471:intron 55.0%
TCAACAGTCGGCGGAGACAA+AGG - chr2.2:50783550-50783569 MS.gene57471:intron 55.0%


Chromosome Type Strat End Strand Name
chr2.2 gene 50780913 50784263 50780913 ID=MS.gene57471
chr2.2 mRNA 50780913 50784263 50780913 ID=MS.gene57471.t1;Parent=MS.gene57471
chr2.2 exon 50784222 50784263 50784222 ID=MS.gene57471.t1.exon1;Parent=MS.gene57471.t1
chr2.2 CDS 50784222 50784263 50784222 ID=cds.MS.gene57471.t1;Parent=MS.gene57471.t1
chr2.2 exon 50783721 50783948 50783721 ID=MS.gene57471.t1.exon2;Parent=MS.gene57471.t1
chr2.2 CDS 50783721 50783948 50783721 ID=cds.MS.gene57471.t1;Parent=MS.gene57471.t1
chr2.2 exon 50780913 50781953 50780913 ID=MS.gene57471.t1.exon3;Parent=MS.gene57471.t1
chr2.2 CDS 50780913 50781953 50780913 ID=cds.MS.gene57471.t1;Parent=MS.gene57471.t1
Gene Sequence

>MS.gene57471

ATGGACATTGCCATGTCTCAGCCATCTTCACACAAAAAAAAGGTATGTCACAAATTTATATAAATTTTTTATATTATTTTCCTTCTTCCTAGGAATTTTTTTTTGATGATTTTAATTGGTATGCATCATGAGTATCTCACAATCAATCATAATTTTTTTTAGTTTTACATTTTTCTAACATTGTTTGAGGATGTAAACAATTTTACAATGACAATGTATCAAAATTACAATTACAGTCTCCTTTTTTTCATTGTATAAAATGTAAAATTACAATCTCATTCTCTCTATGTGGTTTTGGATTGGAAAATGAAGAAGAGTGGAAGAGTGGTGTACCCAATTAAGTATGGTGCAGATCCAACAGGGGAAAATGAAAGCAGTGATGCCATTATGAAAGCTGTAGAAGCTGCATTTGATATTGATAATTTAGGGCTTGAATTATTACCAGGAATTAGAGACCTTGGAGGTGTTATCATTGATTTTCAAGGTGGAAACTATAAAATCAGCAATCCCATCACATTTCCTTCATCGGCTGGCAATCTTGTGGTAATCATCTTTCTCTTAGTTAATTTCTTTCCTTTTCCTCCTTTACTACTTTGATTGATGCAATTTTTTTTACTGCAAGATTAATTATGAACTTGTTGATCCTTAATTTGTTATATAAATTAGCATGTGTTTTTCTAAAATGATTTTTTTTAATATACTTGTAAAAAAATGACAGTTGTCGTTCTCAATAATGTGTTCATTTAACATCAAAATTTATTAAATGTAGTAAATGAGAGAAGTACAAAGAATACTTAACTCAGAACTCGAAAAAAGTACAAGGGATACTCGACCCACCAAAAAACAAGTTAAGAGTTAAAACAAAGAAGAGGGTTCAAAAACTAGTCAAAATACATGCAAAACCTCAATTTTTTCGTAGCTGGAAAACACCTCGAAGAGGTTGATATACGGAGTCTACTATCTCTTCTACCTTCAAGAATCATTCAAGAAAAAATAACCCCATTCCAAATAATGTTTTATTTAAATTATATATATTTTTTGATAATATTTATTAAGAGGAATGTTTTTTTTTTTTTGAAATTATGAACACTTCTTTTCAACGCTCTCTTTAATACTTGTTTCATTGTCTGTCCTATAAATAAATATTTTTCTCTTTTGATTTTTTTTATTGGCGCCAAAATGTTCTTGCATTGAATACTCCAGTGAGCCTGTACCTAGAAGCGTACTAGGAAACACTATCCTGCAATTTCTTAATAACTCCATCAATTTAAGTCTCACTCAACCTCTAACAATTTTGAACCCACTCTCACCAAACAATACTAAAGGGGGCTAACAAGATATGAATACCTTGAGAAATCCAAAGCCTTGTTGGAAAATGAACAAATCTATCGATTCAATAGAATAAAAAAGGTAAAAGTAAACAATAAAAAATGAGATTTTAAAAAAATTATGGAGTGACAAGACTAGATACGAGAGTATAAATAAAATCTTTCAAAAGATGCACAAACCAGACAAAATAAAGGAGATCTGTGTTATCAATTATAATTCAAATTTAATTTGTAAACATGGATAGTGTAAATCTATTTTGCACATTCATCTAATCAGTAGATATTTTTGAAGAGGAATTTTTTTTTTTGAATAAGGAATATATTATTAATATCAATATTCCTTAGCAGTGCAAAACAGATAAAAATGTGTAATATAGAATTTTTCTATGATGTGCAAATTTTTCTATGATGTACATATGTTAGGAAGTTCAAAATAATAAATTTGTATTGGAACTTGTGCATTTTATATTAAATATTATTTTTTAATAAATAATTGTTGCCTTTCAATAAAATTTTGCAACTTTTAGTTCCTTTTAACATCTTACTCCATCCATTCCTTTTCTTTTGATGTTTTAGCTCTTGGCACAAGTTTTAAGGAGTAATTAAAGTATATAAATATTTATGTGTTTTTGACTAAAATGTCCTTATTTATCATTAAATTCACATGTGATAAACCAAAGGTCACATCTACTTTAATAGTTTATGGTGTTTTCCAACAAGGGTAAACTTGAGAAAACAATAATAAATTCACTAAAACATCAAAAGTTTTGGAATATAATTGAAACTCTAAAACATCAAAAGAAAAGGAATGGAGGGAGTAATATGCTTCTTGATAATTGTATAAATCATAACATAACAATATTGGAGGGAAGTTAAATTGCAGTCTTTTTTCTTGAAAAATGCTGCAGTGCTATTTTCTTCTTCCACTTAACTTTTACATATTATTATTTCTTACACTTAACTTTTACATCAATGACCAAAGGTAAATGGAGGAACCTTAAGAGCTTCTGAAACATTTCCAACTGATAGACACCTTGTTGAATTAAATGCATCAAATTCCAAAACCCTCCAAAATGCAACAAAAGCATCCAACAACAGATTACTTGAACAAACAATAGGAATTTACTATGAAGACATAACATTTAGAGACATCCTCTTCGATTCAAGTTACCGAGGTGGAGGAATATTCATCGTCGATTCAGCTAGAATCAGAATCGACAATTGTTTCTTCCTCCATTTCACCACCGAAGGAATTAAAGTTCAAAGTGGCCATGAAACCTTCATATCAAGTAGTTTTCTTGGACAACACTCAACAGTCGGCGGAGACAAAGGTGAAAGACAATTTTCGGGAACTGCAATTGATCTTGCTAGTAATGACAATGCTATCACCGATGTTGCAATTTTCTCAGCAGCCATAGGAATTGTTGTTAGGGGACAAGCTAACATGATCACAGGTGTGCATTGTTATAACAAAGCAACAGGTTTTGGTGGAATTGGAATTTTGTTGAAGTTAGCAGGGAATTCACAAACAAGAATTGATAATTGTTACATGGATTATAATTCTATAGTCATGGAAGATCCTGTTCAAGTTCATGTTACGAATGGTTTTTTCTTAGGGGATGGTAATATTGTTTTGAAATCAATTAAAGGGAAAGTTTATGGCTTAAATATTGTTAACAATATGTTTAGTGGAAACCCTAATAATAATGTTCCAATTGTGAAATTAGATGGGGGATTTAGTGACATTGATCAAGTGGTGATTGATATGAACAATGTGATTGGTATGATTTTAAGATCAACCGTTGGGAAGTTGAATGTGGATGGTAATGGCACAAAATGGGTGGGTGATTTTTCAAATGTTTTGGTATTTCCTAATAGGATTAGTCATTTTCAATATTCATTTCATGCATTAGAGGGACCAAAGTTTGTTGCACATTCAGTGAGTAATGTGTCAAATAATGTTGTAGTTGTGGAAAGTGAGAAACCAGTTCATGGGGTTGTTTCCTTCTTTGTTGAACAATGA
Protein sequence

>MS.gene57471.t1

MDIAMSQPSSHKKKSGRVVYPIKYGADPTGENESSDAIMKAVEAAFDIDNLGLELLPGIRDLGGVIIDFQGGNYKISNPITFPSSAGNLVVNGGTLRASETFPTDRHLVELNASNSKTLQNATKASNNRLLEQTIGIYYEDITFRDILFDSSYRGGGIFIVDSARIRIDNCFFLHFTTEGIKVQSGHETFISSSFLGQHSTVGGDKGERQFSGTAIDLASNDNAITDVAIFSAAIGIVVRGQANMITGVHCYNKATGFGGIGILLKLAGNSQTRIDNCYMDYNSIVMEDPVQVHVTNGFFLGDGNIVLKSIKGKVYGLNIVNNMFSGNPNNNVPIVKLDGGFSDIDQVVIDMNNVIGMILRSTVGKLNVDGNGTKWVGDFSNVLVFPNRISHFQYSFHALEGPKFVAHSVSNVSNNVVVVESEKPVHGVVSFFVEQ