AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene57472

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene57472.t1 MTR_2g035790 91.051 447 29 1 1 436 42 488 0.0 793
MS.gene57472.t1 MTR_4g094732 71.230 431 119 3 9 436 52 480 0.0 615
MS.gene57472.t1 MTR_2g035760 53.269 413 175 7 17 424 82 481 1.45e-142 416
MS.gene57472.t1 MTR_6g015785 52.804 428 178 8 13 436 83 490 1.28e-134 396
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene57472.t1 AT4G20040 63.146 426 150 5 13 436 63 483 0.0 546
MS.gene57472.t1 AT4G20050 54.588 425 169 6 17 436 99 504 2.37e-152 442
MS.gene57472.t1 AT4G20050 54.588 425 169 6 17 436 74 479 3.47e-152 441
MS.gene57472.t1 AT4G20050 54.588 425 169 6 17 436 74 479 3.47e-152 441
MS.gene57472.t1 AT4G20050 54.588 425 169 6 17 436 74 479 3.47e-152 441
MS.gene57472.t1 AT4G20050 54.588 425 169 6 17 436 74 479 3.47e-152 441
MS.gene57472.t1 AT4G20050 54.000 400 160 6 42 436 1 381 9.48e-140 405
MS.gene57472.t1 AT4G20050 54.000 400 160 6 42 436 1 381 9.48e-140 405

Find 83 sgRNAs with CRISPR-Local

Find 179 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
ACCATAAACTTTGCCTTTAA+TGG 0.026748 2.2:+50787755 None:intergenic
GCATTTGATATTGATAATTT+AGG 0.107857 2.2:-50790033 MS.gene57472:CDS
ACAATTGGAACATTATTATT+AGG 0.129299 2.2:+50787699 None:intergenic
TCCATTAAAGGCAAAGTTTA+TGG 0.143235 2.2:-50787756 MS.gene57472:CDS
GGATCTGCACCATACTTAAT+TGG 0.153680 2.2:+50790105 None:intergenic
CAGCCATTGGAATTGTTCTT+AGG 0.195596 2.2:-50787986 MS.gene57472:CDS
CATTTGATATTGATAATTTA+GGG 0.251344 2.2:-50790032 MS.gene57472:CDS
GTTCCAATTGTAAAATTAGA+TGG 0.268649 2.2:-50787687 MS.gene57472:CDS
CATCAATGACCAAAGGTAAA+TGG 0.278889 2.2:-50788428 MS.gene57472:intron
CAATTGGAACATTATTATTA+GGG 0.285274 2.2:+50787700 None:intergenic
TATAACAAAGCAACAGGTTT+TGG 0.286379 2.2:-50787930 MS.gene57472:CDS
TGAAAATGACTAATCCTATT+AGG 0.294977 2.2:+50787525 None:intergenic
ATTGTTAACAATATGTTTAG+TGG 0.301676 2.2:-50787726 MS.gene57472:CDS
GTTCAAGTTCATGTTACTAA+TGG 0.312426 2.2:-50787813 MS.gene57472:CDS
TGGATGGTAATGGCACAAAA+TGG 0.367127 2.2:-50787578 MS.gene57472:CDS
TAATTCCTTCTGTGTTGAAA+TGG 0.369391 2.2:+50788162 None:intergenic
AAATGTTTCAGAAGCTCTTA+AGG 0.373297 2.2:+50788400 None:intergenic
TACTTGATATGAAGGTTTCA+TGG 0.388165 2.2:+50788126 None:intergenic
CCTCCAAGGTCTCTAATTCC+TGG 0.397675 2.2:+50789997 None:intergenic
CATTGTTATAACAAAGCAAC+AGG 0.404226 2.2:-50787936 MS.gene57472:CDS
AGCTTCTACAGCTTTCATAA+TGG 0.424025 2.2:+50790056 None:intergenic
AAGTATGGTGCAGATCCAAC+TGG 0.430294 2.2:-50790099 MS.gene57472:CDS
TTGATCAAGTGGTGATTGAT+AGG 0.431617 2.2:-50787650 MS.gene57472:CDS
AGCCATTGGAATTGTTCTTA+GGG 0.433955 2.2:-50787985 MS.gene57472:CDS
ACTGAATGTGCAACAAACTT+TGG 0.434126 2.2:+50787477 None:intergenic
AAGAAAACTACTTGATATGA+AGG 0.434177 2.2:+50788118 None:intergenic
GTATTCATTTCATGCATTAG+AGG 0.440197 2.2:-50787502 MS.gene57472:CDS
GATCTGCACCATACTTAATT+GGG 0.441924 2.2:+50790106 None:intergenic
TTAGTAACATGAACTTGAAC+AGG 0.445078 2.2:+50787816 None:intergenic
TTGTTCAAGTAATCTGTTGT+TGG 0.449557 2.2:+50788307 None:intergenic
ATCACATTTCCTTCATCGGC+TGG 0.454793 2.2:-50789928 MS.gene57472:CDS
TTCCAATTGTAAAATTAGAT+GGG 0.461356 2.2:-50787686 MS.gene57472:CDS
TCCCATCACATTTCCTTCAT+CGG 0.471050 2.2:-50789932 MS.gene57472:CDS
AAGGAAACAACACCATGAAC+TGG 0.487041 2.2:+50787411 None:intergenic
GATTCGAGCTACCGAGGTGG+AGG 0.488285 2.2:-50788236 MS.gene57472:CDS
TTACCAGGAATTAGAGACCT+TGG 0.494991 2.2:-50790000 MS.gene57472:CDS
CTCGGTAGCTCGAATCGAAA+AGG 0.508146 2.2:+50788243 None:intergenic
TTCCTCCATTTCAACACAGA+AGG 0.508555 2.2:-50788167 MS.gene57472:CDS
AAGAATTGATAATTGTTACA+TGG 0.510868 2.2:-50787865 MS.gene57472:CDS
AACTCATTGTTCAACAAAGA+AGG 0.513850 2.2:+50787392 None:intergenic
TTAGGGCTTGAATTATTACC+AGG 0.514044 2.2:-50790015 MS.gene57472:CDS
TCAACAAGGTATCTATCAGT+TGG 0.515242 2.2:+50788377 None:intergenic
TATTCATTTCATGCATTAGA+GGG 0.516937 2.2:-50787501 MS.gene57472:CDS
GTGGTGTACCCAATTAAGTA+TGG 0.526549 2.2:-50790114 MS.gene57472:CDS
ACAAGATTGCCAGCCGATGA+AGG 0.526613 2.2:+50789919 None:intergenic
GAAAGTGAGAAACCAGTTCA+TGG 0.531426 2.2:-50787423 MS.gene57472:CDS
CTTGGACAACACTCAACAGT+CGG 0.538774 2.2:-50788098 MS.gene57472:CDS
ATTGATAGGAACAATGTGAT+TGG 0.546202 2.2:-50787636 MS.gene57472:CDS
TGGTAATGGCACAAAATGGG+TGG 0.549729 2.2:-50787574 MS.gene57472:CDS
TTCCTTCTGTGTTGAAATGG+AGG 0.550409 2.2:+50788165 None:intergenic
GGATGGTAATGGCACAAAAT+GGG 0.551911 2.2:-50787577 MS.gene57472:CDS
TTCATCGGCTGGCAATCTTG+TGG 0.566505 2.2:-50789917 MS.gene57472:intron
TCCAATTGTAAAATTAGATG+GGG 0.567825 2.2:-50787685 MS.gene57472:CDS
AATGAAGAAGAGTGGAAGAG+TGG 0.571785 2.2:-50790133 MS.gene57472:intron
GGATTATAATAGTATAGTCA+TGG 0.575274 2.2:-50787844 MS.gene57472:CDS
ATTTAGTAACATTGATCAAG+TGG 0.575859 2.2:-50787661 MS.gene57472:CDS
GCCGATGAAGGAAATGTGAT+GGG 0.577733 2.2:+50789931 None:intergenic
AAAGGCGAAAGACAATTCTC+AGG 0.579523 2.2:-50788068 MS.gene57472:CDS
TCAACAGTCGGTGGAGACAA+AGG 0.584797 2.2:-50788086 MS.gene57472:CDS
CCAGGAATTAGAGACCTTGG+AGG 0.587401 2.2:-50789997 MS.gene57472:CDS
GGACAAGCTAACATGATCAC+AGG 0.590351 2.2:-50787963 MS.gene57472:CDS
AGTATGGTGCAGATCCAACT+GGG 0.590855 2.2:-50790098 MS.gene57472:CDS
GCCATTGGAATTGTTCTTAG+GGG 0.599615 2.2:-50787984 MS.gene57472:CDS
TGCCGAGAAAATTGCAACAT+CGG 0.599656 2.2:+50788007 None:intergenic
CCAATTGTAAAATTAGATGG+GGG 0.605323 2.2:-50787684 MS.gene57472:CDS
TTCGATTCGAGCTACCGAGG+TGG 0.606373 2.2:-50788239 MS.gene57472:CDS
AGATTACTTGAACAAACGAT+AGG 0.611568 2.2:-50788299 MS.gene57472:CDS
AGCCGATGAAGGAAATGTGA+TGG 0.612995 2.2:+50789930 None:intergenic
TCCCCTAAGAACAATTCCAA+TGG 0.613399 2.2:+50787983 None:intergenic
AAGTTGAATGTGGATGGTAA+TGG 0.619073 2.2:-50787588 MS.gene57472:CDS
AATCAATGATAACACCTCCA+AGG 0.627415 2.2:+50789983 None:intergenic
AACGGTTGGTAAGTTGAATG+TGG 0.630673 2.2:-50787598 MS.gene57472:CDS
GTGTGAAGATGGCTGAGACA+TGG 0.634929 2.2:+50790428 None:intergenic
GAAGGAATTAAAGTTCAAAG+TGG 0.637402 2.2:-50788149 MS.gene57472:CDS
GTTGGTAAGTTGAATGTGGA+TGG 0.638186 2.2:-50787594 MS.gene57472:CDS
GGTAATGGCACAAAATGGGT+GGG 0.641219 2.2:-50787573 MS.gene57472:CDS
GTTGCTATCAAGATCAATTG+CGG 0.644562 2.2:+50788043 None:intergenic
TTGATGCATTTAATTCAACA+AGG 0.652870 2.2:+50788363 None:intergenic
ATTGTCGATTCTGATTCTAG+CGG 0.659026 2.2:+50788196 None:intergenic
CAATGACCAAAGGTAAATGG+AGG 0.667241 2.2:-50788425 MS.gene57472:intron
GGACAACACTCAACAGTCGG+TGG 0.682248 2.2:-50788095 MS.gene57472:CDS
CGATGAATATTCCTCCACCT+CGG 0.718566 2.2:+50788225 None:intergenic
GTATGGTGCAGATCCAACTG+GGG 0.760947 2.2:-50790097 MS.gene57472:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! CTAATTTATATAACAAATTA+AGG + chr2.2:50788041-50788060 None:intergenic 10.0%
!! TCAAAATAATAAATTTGTAT+TGG - chr2.2:50789154-50789173 MS.gene57472:intron 10.0%
!!! ATGAGATTTTAAAAAAATTA+TGG - chr2.2:50788816-50788835 MS.gene57472:intron 10.0%
!!! TTTTGATAATATTTATTAAG+AGG - chr2.2:50788439-50788458 MS.gene57472:intron 10.0%
!!! TTTTTTTTGATGATTTTAAT+TGG - chr2.2:50787490-50787509 MS.gene57472:CDS 10.0%
!! AAATAAAACATTATTTGGAA+TGG + chr2.2:50788405-50788424 None:intergenic 15.0%
!! AATAATATGTAAAAGTTAAG+TGG + chr2.2:50789364-50789383 None:intergenic 15.0%
!!! AATAAAACATTATTTGGAAT+GGG + chr2.2:50788404-50788423 None:intergenic 15.0%
!!! CATTTGATATTGATAATTTA+GGG - chr2.2:50787801-50787820 MS.gene57472:CDS 15.0%
!!! CATTTTATACAATGAAAAAA+AGG + chr2.2:50787637-50787656 None:intergenic 15.0%
!!! TCAAATTTAATTTGTAAACA+TGG - chr2.2:50788932-50788951 MS.gene57472:intron 15.0%
!!! TCTCTTTTGATTTTTTTTAT+TGG - chr2.2:50788535-50788554 MS.gene57472:intron 15.0%
!! AAGCATATTAAGATGTTAAA+AGG + chr2.2:50789258-50789277 None:intergenic 20.0%
!! ACAATTGGAACATTATTATT+AGG + chr2.2:50790137-50790156 None:intergenic 20.0%
!! ATTGTTAACAATATGTTTAG+TGG - chr2.2:50790107-50790126 MS.gene57472:CDS 20.0%
!! CAATTGGAACATTATTATTA+GGG + chr2.2:50790136-50790155 None:intergenic 20.0%
!! CATAACATAACAATATTTGA+GGG - chr2.2:50789296-50789315 MS.gene57472:intron 20.0%
!! TCATAACATAACAATATTTG+AGG - chr2.2:50789295-50789314 MS.gene57472:intron 20.0%
!! TCGATTCAATAGAATAAAAA+AGG - chr2.2:50788774-50788793 MS.gene57472:intron 20.0%
!! TTCCAATTGTAAAATTAGAT+GGG - chr2.2:50790147-50790166 MS.gene57472:intron 20.0%
!!! AAGAATTGATAATTGTTACA+TGG - chr2.2:50789968-50789987 MS.gene57472:CDS 20.0%
!!! AGTTGCAAAATTTTATTGAT+AGG + chr2.2:50789228-50789247 None:intergenic 20.0%
!!! ATAAAACATTATTTGGAATG+GGG + chr2.2:50788403-50788422 None:intergenic 20.0%
!!! ATATTATTTTCCTTTTTCCT+AGG - chr2.2:50787465-50787484 MS.gene57472:CDS 20.0%
!!! GCATTTGATATTGATAATTT+AGG - chr2.2:50787800-50787819 MS.gene57472:CDS 20.0%
!!! TTAACTAAGTATTTGTTTTC+TGG + chr2.2:50788234-50788253 None:intergenic 20.0%
!!! TTACTAATGGTTTTTTCTTA+GGG - chr2.2:50790033-50790052 MS.gene57472:CDS 20.0%
! AAAAAAAACTCCTAGGAAAA+AGG + chr2.2:50787478-50787497 None:intergenic 25.0%
! AAGAAAACTACTTGATATGA+AGG + chr2.2:50789718-50789737 None:intergenic 25.0%
! AAGAGTTAAAACAAAGAAGA+GGG - chr2.2:50788252-50788271 MS.gene57472:CDS 25.0%
! AATCATCAAAAAAAACTCCT+AGG + chr2.2:50787485-50787504 None:intergenic 25.0%
! ATTTAGTAACATTGATCAAG+TGG - chr2.2:50790172-50790191 MS.gene57472:intron 25.0%
! GGATTATAATAGTATAGTCA+TGG - chr2.2:50789989-50790008 MS.gene57472:CDS 25.0%
! GTTCCAATTGTAAAATTAGA+TGG - chr2.2:50790146-50790165 MS.gene57472:intron 25.0%
! TAAGAGTTAAAACAAAGAAG+AGG - chr2.2:50788251-50788270 MS.gene57472:CDS 25.0%
! TATTCATTTCATGCATTAGA+GGG - chr2.2:50790332-50790351 MS.gene57472:intron 25.0%
! TCCAATTGTAAAATTAGATG+GGG - chr2.2:50790148-50790167 MS.gene57472:intron 25.0%
! TGAAAATGACTAATCCTATT+AGG + chr2.2:50790311-50790330 None:intergenic 25.0%
! TTAAATGTAGTATATGAGAG+AGG - chr2.2:50788154-50788173 MS.gene57472:CDS 25.0%
! TTCTATGATGTACATATGTT+AGG - chr2.2:50789127-50789146 MS.gene57472:intron 25.0%
! TTGATGCATTTAATTCAACA+AGG + chr2.2:50789473-50789492 None:intergenic 25.0%
!! CATTTTTCTAACATTGTTTG+AGG - chr2.2:50787562-50787581 MS.gene57472:CDS 25.0%
!! GAATTTTGTTGAAATTAGCA+GGG - chr2.2:50789934-50789953 MS.gene57472:CDS 25.0%
!! TATTCAATGCAAGAACATTT+TGG + chr2.2:50788563-50788582 None:intergenic 25.0%
!! TTCATATCAAGTAGTTTTCT+TGG - chr2.2:50789717-50789736 MS.gene57472:intron 25.0%
!! TTTTCTTGAATGATTCTTGA+AGG + chr2.2:50788375-50788394 None:intergenic 25.0%
!!! ATATTTTGGTGTTTCCTAAT+AGG - chr2.2:50790294-50790313 MS.gene57472:intron 25.0%
!!! ATTGTTTTGAAGTCCATTAA+AGG - chr2.2:50790065-50790084 MS.gene57472:CDS 25.0%
!!! GGGTGATTTTTCAAATATTT+TGG - chr2.2:50790280-50790299 MS.gene57472:intron 25.0%
!!! GTTACTAATGGTTTTTTCTT+AGG - chr2.2:50790032-50790051 MS.gene57472:CDS 25.0%
!!! TACTAATGGTTTTTTCTTAG+GGG - chr2.2:50790034-50790053 MS.gene57472:CDS 25.0%
!!! TGGTATGATTTTAAGATCAA+CGG - chr2.2:50790217-50790236 MS.gene57472:intron 25.0%
AAATGTTTCAGAAGCTCTTA+AGG + chr2.2:50789436-50789455 None:intergenic 30.0%
ACCATAAACTTTGCCTTTAA+TGG + chr2.2:50790081-50790100 None:intergenic 30.0%
AGATTACTTGAACAAACGAT+AGG - chr2.2:50789534-50789553 MS.gene57472:intron 30.0%
ATCAATCAAAGTAGTAAAGG+AGG + chr2.2:50787977-50787996 None:intergenic 30.0%
ATTGATAGGAACAATGTGAT+TGG - chr2.2:50790197-50790216 MS.gene57472:intron 30.0%
CATTGTTATAACAAAGCAAC+AGG - chr2.2:50789897-50789916 MS.gene57472:intron 30.0%
CCAATTGTAAAATTAGATGG+GGG - chr2.2:50790149-50790168 MS.gene57472:intron 30.0%
GAAGGAATTAAAGTTCAAAG+TGG - chr2.2:50789684-50789703 MS.gene57472:intron 30.0%
GTATTCATTTCATGCATTAG+AGG - chr2.2:50790331-50790350 MS.gene57472:intron 30.0%
TAATTCCTTCTGTGTTGAAA+TGG + chr2.2:50789674-50789693 None:intergenic 30.0%
TACTTGATATGAAGGTTTCA+TGG + chr2.2:50789710-50789729 None:intergenic 30.0%
TCCATTAAAGGCAAAGTTTA+TGG - chr2.2:50790077-50790096 MS.gene57472:CDS 30.0%
TCTCACCAAACAATACTAAA+GGG - chr2.2:50788690-50788709 MS.gene57472:intron 30.0%
TGCATCAATCAAAGTAGTAA+AGG + chr2.2:50787980-50787999 None:intergenic 30.0%
TGGAGTTATTAAGAAATTGC+AGG + chr2.2:50788628-50788647 None:intergenic 30.0%
TTAGTAACATGAACTTGAAC+AGG + chr2.2:50790020-50790039 None:intergenic 30.0%
TTGAGTGAGACTTAAATTGA+TGG + chr2.2:50788648-50788667 None:intergenic 30.0%
TTGTTCAAGTAATCTGTTGT+TGG + chr2.2:50789529-50789548 None:intergenic 30.0%
! ACTTTTACATCAATGACCAA+AGG - chr2.2:50789398-50789417 MS.gene57472:intron 30.0%
! AGAACTCGAAAAAAGTACAA+GGG - chr2.2:50788196-50788215 MS.gene57472:CDS 30.0%
! GGAATTTTGTTGAAATTAGC+AGG - chr2.2:50789933-50789952 MS.gene57472:CDS 30.0%
! GTTCAAGTTCATGTTACTAA+TGG - chr2.2:50790020-50790039 MS.gene57472:CDS 30.0%
! TATAACAAAGCAACAGGTTT+TGG - chr2.2:50789903-50789922 MS.gene57472:intron 30.0%
!! ACTAAGTATTTGTTTTCTGG+TGG + chr2.2:50788231-50788250 None:intergenic 30.0%
!! CTAAGTATTTGTTTTCTGGT+GGG + chr2.2:50788230-50788249 None:intergenic 30.0%
!! GATTTGTTCATTTTCCAACA+AGG + chr2.2:50788752-50788771 None:intergenic 30.0%
!! TCACGTAGATATTTTTGAAG+AGG - chr2.2:50788996-50789015 MS.gene57472:intron 30.0%
!!! ACCTTTTTTTTGTGTGAAGA+TGG + chr2.2:50787419-50787438 None:intergenic 30.0%
!!! ATGATTTTAAGATCAACGGT+TGG - chr2.2:50790221-50790240 MS.gene57472:intron 30.0%
!!! GGTGTTATCATTGATTTTCA+AGG - chr2.2:50787857-50787876 MS.gene57472:CDS 30.0%
!!! GTTATCATTGATTTTCAAGG+TGG - chr2.2:50787860-50787879 MS.gene57472:CDS 30.0%
AAGTTGAATGTGGATGGTAA+TGG - chr2.2:50790245-50790264 MS.gene57472:intron 35.0%
AATCAATGATAACACCTCCA+AGG + chr2.2:50787853-50787872 None:intergenic 35.0%
ACTGAATGTGCAACAAACTT+TGG + chr2.2:50790359-50790378 None:intergenic 35.0%
AGCCATTGGAATTGTTCTTA+GGG - chr2.2:50789848-50789867 MS.gene57472:intron 35.0%
AGCTTCTACAGCTTTCATAA+TGG + chr2.2:50787780-50787799 None:intergenic 35.0%
CAAAGTAGTAAAGGAGGAAA+AGG + chr2.2:50787971-50787990 None:intergenic 35.0%
CATCAATGACCAAAGGTAAA+TGG - chr2.2:50789405-50789424 MS.gene57472:intron 35.0%
CTCACCAAACAATACTAAAG+GGG - chr2.2:50788691-50788710 MS.gene57472:intron 35.0%
CTCTCACCAAACAATACTAA+AGG - chr2.2:50788689-50788708 MS.gene57472:intron 35.0%
GATCTGCACCATACTTAATT+GGG + chr2.2:50787730-50787749 None:intergenic 35.0%
GATTGGAAAATGAAGAAGAG+TGG - chr2.2:50787692-50787711 MS.gene57472:CDS 35.0%
GCACAAACCAGACAAAATAA+AGG - chr2.2:50788886-50788905 MS.gene57472:intron 35.0%
GCCATCTTCACACAAAAAAA+AGG - chr2.2:50787415-50787434 MS.gene57472:CDS 35.0%
GTTGCTATCAAGATCAATTG+CGG + chr2.2:50789793-50789812 None:intergenic 35.0%
TCACCAAACAATACTAAAGG+GGG - chr2.2:50788692-50788711 MS.gene57472:intron 35.0%
TTAGGGCTTGAATTATTACC+AGG - chr2.2:50787818-50787837 MS.gene57472:CDS 35.0%
TTCCAGCTAGGAAAAAATTG+AGG + chr2.2:50788307-50788326 None:intergenic 35.0%
TTGATCAAGTGGTGATTGAT+AGG - chr2.2:50790183-50790202 MS.gene57472:intron 35.0%
! AGTGGGTTCAAAATTGTTAG+AGG + chr2.2:50788671-50788690 None:intergenic 35.0%
! ATGCTTTTGTTGCATTTTGG+AGG + chr2.2:50789506-50789525 None:intergenic 35.0%
! CAGAACTCGAAAAAAGTACA+AGG - chr2.2:50788195-50788214 MS.gene57472:CDS 35.0%
! CCCCCATCTAATTTTACAAT+TGG + chr2.2:50790152-50790171 None:intergenic 35.0%
! TACCGATGTTGCAATTTTCT+CGG - chr2.2:50789824-50789843 MS.gene57472:intron 35.0%
! TAGTATTGTTTGGTGAGAGT+GGG + chr2.2:50788688-50788707 None:intergenic 35.0%
! TCAACAAGGTATCTATCAGT+TGG + chr2.2:50789459-50789478 None:intergenic 35.0%
! TGCTTTTGTTGCATTTTGGA+GGG + chr2.2:50789505-50789524 None:intergenic 35.0%
! TGGATGCTTTTGTTGCATTT+TGG + chr2.2:50789509-50789528 None:intergenic 35.0%
! TTAGTATTGTTTGGTGAGAG+TGG + chr2.2:50788689-50788708 None:intergenic 35.0%
! TTCATTTTCCAACAAGGCTT+TGG + chr2.2:50788746-50788765 None:intergenic 35.0%
!! ATTGTCGATTCTGATTCTAG+CGG + chr2.2:50789640-50789659 None:intergenic 35.0%
!!! AACCTCAATTTTTTCCTAGC+TGG - chr2.2:50788302-50788321 MS.gene57472:CDS 35.0%
!!! CAGATCTCCTTTATTTTGTC+TGG + chr2.2:50788896-50788915 None:intergenic 35.0%
AAAGGCGAAAGACAATTCTC+AGG - chr2.2:50789765-50789784 MS.gene57472:intron 40.0%
AAGGAAACAACACCATGAAC+TGG + chr2.2:50790425-50790444 None:intergenic 40.0%
AAGGTTCCTCCATTTACCTT+TGG + chr2.2:50789417-50789436 None:intergenic 40.0%
AATGAAGAAGAGTGGAAGAG+TGG - chr2.2:50787700-50787719 MS.gene57472:CDS 40.0%
ACAATCTCATTCTCTCTGTG+TGG - chr2.2:50787664-50787683 MS.gene57472:CDS 40.0%
CAATGACCAAAGGTAAATGG+AGG - chr2.2:50789408-50789427 MS.gene57472:intron 40.0%
CAGCCATTGGAATTGTTCTT+AGG - chr2.2:50789847-50789866 MS.gene57472:intron 40.0%
GAAAGTGAGAAACCAGTTCA+TGG - chr2.2:50790410-50790429 MS.gene57472:intron 40.0%
GCCATTGGAATTGTTCTTAG+GGG - chr2.2:50789849-50789868 MS.gene57472:intron 40.0%
GGATCTGCACCATACTTAAT+TGG + chr2.2:50787731-50787750 None:intergenic 40.0%
GTGGTGTACCCAATTAAGTA+TGG - chr2.2:50787719-50787738 MS.gene57472:CDS 40.0%
TAGCCCCCTTTAGTATTGTT+TGG + chr2.2:50788698-50788717 None:intergenic 40.0%
TCCCATCACATTTCCTTCAT+CGG - chr2.2:50787901-50787920 MS.gene57472:CDS 40.0%
TCCCCTAAGAACAATTCCAA+TGG + chr2.2:50789853-50789872 None:intergenic 40.0%
TGCCGAGAAAATTGCAACAT+CGG + chr2.2:50789829-50789848 None:intergenic 40.0%
TTACCAGGAATTAGAGACCT+TGG - chr2.2:50787833-50787852 MS.gene57472:CDS 40.0%
TTCCTCCATTTCAACACAGA+AGG - chr2.2:50789666-50789685 MS.gene57472:intron 40.0%
TTCCTTCTGTGTTGAAATGG+AGG + chr2.2:50789671-50789690 None:intergenic 40.0%
! GTTGGTAAGTTGAATGTGGA+TGG - chr2.2:50790239-50790258 MS.gene57472:intron 40.0%
! TCATTCTCTCTGTGTGGTTT+TGG - chr2.2:50787670-50787689 MS.gene57472:CDS 40.0%
! TGAGAAATCCAAAGCCTTGT+TGG - chr2.2:50788735-50788754 MS.gene57472:intron 40.0%
! TTCTCTGTTTTGCACTGCTA+AGG + chr2.2:50789068-50789087 None:intergenic 40.0%
!! AACAAAGCAACAGGTTTTGG+TGG - chr2.2:50789906-50789925 MS.gene57472:intron 40.0%
!! AACGGTTGGTAAGTTGAATG+TGG - chr2.2:50790235-50790254 MS.gene57472:intron 40.0%
!! ACAAGGCTTTGGATTTCTCA+AGG + chr2.2:50788735-50788754 None:intergenic 40.0%
!! CCTCGAAGAGGTTGATATTA+CGG - chr2.2:50788331-50788350 MS.gene57472:CDS 40.0%
!! GGATGGTAATGGCACAAAAT+GGG - chr2.2:50790256-50790275 MS.gene57472:intron 40.0%
!! TGGATGGTAATGGCACAAAA+TGG - chr2.2:50790255-50790274 MS.gene57472:intron 40.0%
!! TGTTGCATTTTGGAGGGTTT+TGG + chr2.2:50789499-50789518 None:intergenic 40.0%
AAGTATGGTGCAGATCCAAC+TGG - chr2.2:50787734-50787753 MS.gene57472:CDS 45.0%
AGCCGATGAAGGAAATGTGA+TGG + chr2.2:50787906-50787925 None:intergenic 45.0%
AGTATGGTGCAGATCCAACT+GGG - chr2.2:50787735-50787754 MS.gene57472:CDS 45.0%
ATCACATTTCCTTCATCGGC+TGG - chr2.2:50787905-50787924 MS.gene57472:CDS 45.0%
CCGTAATATCAACCTCTTCG+AGG + chr2.2:50788334-50788353 None:intergenic 45.0%
CGATGAATATTCCTCCACCT+CGG + chr2.2:50789611-50789630 None:intergenic 45.0%
CTTGGACAACACTCAACAGT+CGG - chr2.2:50789735-50789754 MS.gene57472:intron 45.0%
GCCGATGAAGGAAATGTGAT+GGG + chr2.2:50787905-50787924 None:intergenic 45.0%
GGACAAGCTAACATGATCAC+AGG - chr2.2:50789870-50789889 MS.gene57472:intron 45.0%
GTGTTTCCTAGTACGCTTCT+AGG + chr2.2:50788602-50788621 None:intergenic 45.0%
! CTGCTTTCTTTTTCCCCAGT+TGG + chr2.2:50787752-50787771 None:intergenic 45.0%
! GGTAATGGCACAAAATGGGT+GGG - chr2.2:50790260-50790279 MS.gene57472:intron 45.0%
!! GCAACAGGTTTTGGTGGAAT+TGG - chr2.2:50789912-50789931 MS.gene57472:intron 45.0%
!! TGGTAATGGCACAAAATGGG+TGG - chr2.2:50790259-50790278 MS.gene57472:intron 45.0%
!!! CTCTCTGTGTGGTTTTGGAT+TGG - chr2.2:50787675-50787694 MS.gene57472:CDS 45.0%
!! AATTTAAATAAAACATTATT+TGG + chr2.2:50788410-50788429 None:intergenic 5.0%
!!! ATTTTTTTTTTTTTTGAATA+AGG - chr2.2:50789019-50789038 MS.gene57472:intron 5.0%
ACAAGATTGCCAGCCGATGA+AGG + chr2.2:50787917-50787936 None:intergenic 50.0%
AGCTGGAAAACACCTCGAAG+AGG - chr2.2:50788319-50788338 MS.gene57472:CDS 50.0%
CCAGGAATTAGAGACCTTGG+AGG - chr2.2:50787836-50787855 MS.gene57472:CDS 50.0%
CCTAGTACGCTTCTAGGTAC+AGG + chr2.2:50788596-50788615 None:intergenic 50.0%
CCTCCAAGGTCTCTAATTCC+TGG + chr2.2:50787839-50787858 None:intergenic 50.0%
CTCGGTAGCTCGAATCGAAA+AGG + chr2.2:50789593-50789612 None:intergenic 50.0%
GTATGGTGCAGATCCAACTG+GGG - chr2.2:50787736-50787755 MS.gene57472:CDS 50.0%
GTGTGAAGATGGCTGAGACA+TGG + chr2.2:50787408-50787427 None:intergenic 50.0%
TCAACAGTCGGTGGAGACAA+AGG - chr2.2:50789747-50789766 MS.gene57472:intron 50.0%
! CCTGTACCTAGAAGCGTACT+AGG - chr2.2:50788593-50788612 MS.gene57472:intron 50.0%
! GCAATTTTCTCGGCAGCCAT+TGG - chr2.2:50789834-50789853 MS.gene57472:intron 50.0%
! TTCATCGGCTGGCAATCTTG+TGG - chr2.2:50787916-50787935 MS.gene57472:CDS 50.0%
!! CTTCGAGGTGTTTTCCAGCT+AGG + chr2.2:50788319-50788338 None:intergenic 50.0%
!! CTTTTCGATTCGAGCTACCG+AGG - chr2.2:50789591-50789610 MS.gene57472:intron 50.0%
GCTTCTAGGTACAGGCTCAC+TGG + chr2.2:50788588-50788607 None:intergenic 55.0%
GGACAACACTCAACAGTCGG+TGG - chr2.2:50789738-50789757 MS.gene57472:intron 55.0%
! TTCGATTCGAGCTACCGAGG+TGG - chr2.2:50789594-50789613 MS.gene57472:intron 55.0%
GATTCGAGCTACCGAGGTGG+AGG - chr2.2:50789597-50789616 MS.gene57472:intron 60.0%


Chromosome Type Strat End Strand Name
chr2.2 gene 50787395 50790460 50787395 ID=MS.gene57472
chr2.2 mRNA 50787395 50790460 50787395 ID=MS.gene57472.t1;Parent=MS.gene57472
chr2.2 exon 50790419 50790460 50790419 ID=MS.gene57472.t1.exon1;Parent=MS.gene57472.t1
chr2.2 CDS 50790419 50790460 50790419 ID=cds.MS.gene57472.t1;Parent=MS.gene57472.t1
chr2.2 exon 50789918 50790145 50789918 ID=MS.gene57472.t1.exon2;Parent=MS.gene57472.t1
chr2.2 CDS 50789918 50790145 50789918 ID=cds.MS.gene57472.t1;Parent=MS.gene57472.t1
chr2.2 exon 50787395 50788435 50787395 ID=MS.gene57472.t1.exon3;Parent=MS.gene57472.t1
chr2.2 CDS 50787395 50788435 50787395 ID=cds.MS.gene57472.t1;Parent=MS.gene57472.t1
Gene Sequence

>MS.gene57472

ATGGACATTGCCATGTCTCAGCCATCTTCACACAAAAAAAAGGTATGTCACAAATTTATATAAATTTTTTATATTATTTTCCTTTTTCCTAGGAGTTTTTTTTGATGATTTTAATTGGTATGCATCATGAGTATCTCACAATCAATCATAATTTTTTTTTAGTTTTACATTTTTCTAACATTGTTTGAGGATGTAAACAATTTTACAATGACAATGTATCAAAATTACAATTACAATCTCCTTTTTTTCATTGTATAAAATGTAAAATTACAATCTCATTCTCTCTGTGTGGTTTTGGATTGGAAAATGAAGAAGAGTGGAAGAGTGGTGTACCCAATTAAGTATGGTGCAGATCCAACTGGGGAAAAAGAAAGCAGTGATGCCATTATGAAAGCTGTAGAAGCTGCATTTGATATTGATAATTTAGGGCTTGAATTATTACCAGGAATTAGAGACCTTGGAGGTGTTATCATTGATTTTCAAGGTGGAAACTATAAAATCAGCAATCCCATCACATTTCCTTCATCGGCTGGCAATCTTGTGGTAATCATCTTTCTCTTAGTTAATTTCTTTCCTTTTCCTCCTTTACTACTTTGATTGATGCAATTTTTTTTACTGCAAGATTAATTATGAACTTGTTGATCCTTAATTTGTTATATAAATTAGCATGTGTTTTGCTAAAATGATTTTTTTTAATATACTTGTAAAAAAATGACAGTTGTCGTTCTCAATAATGTGTTCATTTAACATCAAAATTTATTAAATGTAGTATATGAGAGAGGTACAAAGAATACTTAACTCAGAACTCGAAAAAAGTACAAGGGATATTCGACCCACCAGAAAACAAATACTTAGTTAAGAGTTAAAACAAAGAAGAGGGTTCAAAAACTAGTCAAAATACATGCAAAACCTCAATTTTTTCCTAGCTGGAAAACACCTCGAAGAGGTTGATATTACGGAGTCTACTATCTCTTCTACCTTCAAGAATCATTCAAGAAAAAATAACCCCATTCCAAATAATGTTTTATTTAAATTATATATTTTTTTTGATAATATTTATTAAGAGGAATGTTATGAACACTTCTTTTCAACGCTCTCTTTAATACTTGTTTCATTGTCTGTCTTATAAATAAATATTTTTCTCTTTTGATTTTTTTTATTGGCGCCAAAATGTTCTTGCATTGAATACTCCAGTGAGCCTGTACCTAGAAGCGTACTAGGAAACACTATCCTGCAATTTCTTAATAACTCCATCAATTTAAGTCTCACTCAACCTCTAACAATTTTGAACCCACTCTCACCAAACAATACTAAAGGGGGCTAACAAGATATGAATACCTTGAGAAATCCAAAGCCTTGTTGGAAAATGAACAAATCTATCGATTCAATAGAATAAAAAAGGTAAAAGTAAACAATAAAAAATGAGATTTTAAAAAAATTATGGAGTGACAAGACTAGATACGAGAGTATAAATAAAATCTTTCAAAAGATGCACAAACCAGACAAAATAAAGGAGATCTGTGTTATCAATTATAATTCAAATTTAATTTGTAAACATGGATAGTGTAAATCTATATATTTTGCACATTCATCTAATCAAATCACGTAGATATTTTTGAAGAGGATTTTTTTTTTTTTTGAATAAGGAATATATTATTAATATCAATATTCCTTAGCAGTGCAAAACAGAGAAAAATGTGTAATATAGAATTTTTCTATGATGTGCAAATTTTTCTATGATGTACATATGTTAGGAAGTTCAAAATAATAAATTTGTATTGGAACTTGTGCATTTTATATTAAATATTATTTTTTAATAAATAATTGTTGCCTATCAATAAAATTTTGCAACTTTTAGTTCCTTTTAACATCTTAATATGCTTCTTGATGATTGTATAAATCATAACATAACAATATTTGAGGGAGTCTTTTTTCTTGAAAAATGCTGCAGTGCTATTTTCTTCTTCCACTTAACTTTTACATATTATTATTTCTTACACTTAACTTTTACATCAATGACCAAAGGTAAATGGAGGAACCTTAAGAGCTTCTGAAACATTTCCAACTGATAGATACCTTGTTGAATTAAATGCATCAAATTCCAAAACCCTCCAAAATGCAACAAAAGCATCCAACAACAGATTACTTGAACAAACGATAGGATTTTACTATGAAGACATAACATTTAGAGACATCCTTTTCGATTCGAGCTACCGAGGTGGAGGAATATTCATCGTCGATTCCGCTAGAATCAGAATCGACAATTGTTTCTTCCTCCATTTCAACACAGAAGGAATTAAAGTTCAAAGTGGCCATGAAACCTTCATATCAAGTAGTTTTCTTGGACAACACTCAACAGTCGGTGGAGACAAAGGCGAAAGACAATTCTCAGGAACCGCAATTGATCTTGATAGCAACGACAATGCAGTTACCGATGTTGCAATTTTCTCGGCAGCCATTGGAATTGTTCTTAGGGGACAAGCTAACATGATCACAGGTGTGCATTGTTATAACAAAGCAACAGGTTTTGGTGGAATTGGAATTTTGTTGAAATTAGCAGGGAATTCACAAACAAGAATTGATAATTGTTACATGGATTATAATAGTATAGTCATGGAAGATCCTGTTCAAGTTCATGTTACTAATGGTTTTTTCTTAGGGGATGCTAATATTGTTTTGAAGTCCATTAAAGGCAAAGTTTATGGTTTAAATATTGTTAACAATATGTTTAGTGGAAACCCTAATAATAATGTTCCAATTGTAAAATTAGATGGGGGATTTAGTAACATTGATCAAGTGGTGATTGATAGGAACAATGTGATTGGTATGATTTTAAGATCAACGGTTGGTAAGTTGAATGTGGATGGTAATGGCACAAAATGGGTGGGTGATTTTTCAAATATTTTGGTGTTTCCTAATAGGATTAGTCATTTTCAGTATTCATTTCATGCATTAGAGGGACCAAAGTTTGTTGCACATTCAGTGAGTAATGTGTCAAATAATGTTGTAGTTGTTGAAAGTGAGAAACCAGTTCATGGTGTTGTTTCCTTCTTTGTTGAACAATGA
Protein sequence

>MS.gene57472.t1

MDIAMSQPSSHKKKSGRVVYPIKYGADPTGEKESSDAIMKAVEAAFDIDNLGLELLPGIRDLGGVIIDFQGGNYKISNPITFPSSAGNLVVNGGTLRASETFPTDRYLVELNASNSKTLQNATKASNNRLLEQTIGFYYEDITFRDILFDSSYRGGGIFIVDSARIRIDNCFFLHFNTEGIKVQSGHETFISSSFLGQHSTVGGDKGERQFSGTAIDLDSNDNAVTDVAIFSAAIGIVLRGQANMITGVHCYNKATGFGGIGILLKLAGNSQTRIDNCYMDYNSIVMEDPVQVHVTNGFFLGDANIVLKSIKGKVYGLNIVNNMFSGNPNNNVPIVKLDGGFSNIDQVVIDRNNVIGMILRSTVGKLNVDGNGTKWVGDFSNILVFPNRISHFQYSFHALEGPKFVAHSVSNVSNNVVVVESEKPVHGVVSFFVEQ