AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene57478

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene57478.t1 MTR_2g035760 96.930 456 14 0 1 456 42 497 0.0 904
MS.gene57478.t1 MTR_6g015785 72.867 457 116 3 1 456 45 494 0.0 631
MS.gene57478.t1 MTR_4g094732 46.467 467 219 10 2 452 29 480 3.45e-140 410
MS.gene57478.t1 MTR_2g035790 52.113 426 184 7 41 452 69 488 4.99e-137 403
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene57478.t1 AT4G20050 61.842 456 157 4 1 454 66 506 0.0 565
MS.gene57478.t1 AT4G20050 61.842 456 157 5 1 454 41 481 0.0 564
MS.gene57478.t1 AT4G20050 61.842 456 157 5 1 454 41 481 0.0 564
MS.gene57478.t1 AT4G20050 61.842 456 157 5 1 454 41 481 0.0 564
MS.gene57478.t1 AT4G20050 61.842 456 157 5 1 454 41 481 0.0 564
MS.gene57478.t1 AT4G20050 64.962 391 127 3 66 454 1 383 0.0 517
MS.gene57478.t1 AT4G20050 64.962 391 127 3 66 454 1 383 0.0 517
MS.gene57478.t1 AT4G20040 49.173 423 198 4 41 452 67 483 2.82e-137 403

Find 96 sgRNAs with CRISPR-Local

Find 167 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
AACTCTCAAGGATCTCTTAT+TGG 0.214387 2.2:-50835824 MS.gene57478:CDS
TGGTGTACATTGTTACAATA+AGG 0.274737 2.2:-50835509 MS.gene57478:CDS
CATAGATTTCAACACAATAT+TGG 0.278645 2.2:+50835339 None:intergenic
TGCGACGGGGATGAATTTAA+AGG 0.314297 2.2:-50835182 MS.gene57478:CDS
TACAATAAGGCCACTGGATT+CGG 0.316472 2.2:-50835496 MS.gene57478:CDS
AGGTGTTGAAATTGTTCATT+TGG 0.332781 2.2:-50835260 MS.gene57478:CDS
TTATTGGATTCAAACTTTAG+AGG 0.335089 2.2:-50835808 MS.gene57478:CDS
AGTTTCTTATGTATGTTTCA+TGG 0.352507 2.2:+50835692 None:intergenic
ACTTGATCAACTTGATGAAA+TGG 0.356705 2.2:+50835223 None:intergenic
TGCTATCTGAATTGCCTGTT+GGG 0.359312 2.2:+50836784 None:intergenic
TTGATCAAGTTATTGTTGAT+AGG 0.379420 2.2:-50835210 MS.gene57478:CDS
ATGTTCCCAACGCTGGTTAC+CGG 0.382500 2.2:+50836621 None:intergenic
ATTGTTGATAACATGTTCTC+TGG 0.395023 2.2:-50835292 MS.gene57478:CDS
CTCTGCAGCTATAGGAATCA+TGG 0.414635 2.2:-50835557 MS.gene57478:CDS
GCCACTGGATTCGGCGGTAC+CGG 0.416083 2.2:-50835487 MS.gene57478:CDS
CAGTACTCATTAAGTTCAAC+AGG 0.423624 2.2:-50835064 MS.gene57478:CDS
ACATTTCTGATAGCATGATT+AGG 0.424152 2.2:+50835031 None:intergenic
CAGAAAGTCACTCAAGTTAC+CGG 0.424279 2.2:-50836640 MS.gene57478:CDS
CTAGAAATATGAAGTTGAAC+AGG 0.426194 2.2:+50835382 None:intergenic
GCTATAGGAATCATGGTAAC+TGG 0.427133 2.2:-50835550 MS.gene57478:CDS
ATATCCTTCGCTTGGACCAT+TGG 0.432642 2.2:+50836728 None:intergenic
TACATAAGAAACTCTTTCCT+TGG 0.440272 2.2:-50835682 MS.gene57478:CDS
TCAATGATGTAGCCATCATT+AGG 0.440588 2.2:+50835916 None:intergenic
ATTTCTAGTAGTTTCTTCCT+TGG 0.441880 2.2:-50835367 MS.gene57478:CDS
ACCTCAGCTGGTTGAGAAGA+AGG 0.449049 2.2:+50837011 None:intergenic
TCCATCAAATATCCTTCGCT+TGG 0.449157 2.2:+50836720 None:intergenic
TGCAAGGGAATGGAACTTCA+TGG 0.449669 2.2:-50835138 MS.gene57478:CDS
GTTGATAGGAACGTTGCGAC+GGG 0.451880 2.2:-50835196 MS.gene57478:CDS
ATTACACATTTCACAACCAA+TGG 0.452369 2.2:-50835733 MS.gene57478:CDS
ACCTCATCAAATGAAAATGA+TGG 0.468336 2.2:-50835886 MS.gene57478:CDS
GATGTCACGTGGTATACTCT+TGG 0.468628 2.2:+50836819 None:intergenic
CCTTTCGTGTTTATCGCCTC+CGG 0.470986 2.2:+50835645 None:intergenic
GGAATCATGTCTTGTTAAAG+AGG 0.474247 2.2:+50837070 None:intergenic
TCCAAGCGAAGGATATTTGA+TGG 0.475862 2.2:-50836721 MS.gene57478:CDS
GTGCTATCTGAATTGCCTGT+TGG 0.477189 2.2:+50836783 None:intergenic
CGCCTCCGGCGGTTATGTGT+TGG 0.483145 2.2:+50835659 None:intergenic
TGATTGAATTATACCTCAGC+TGG 0.483711 2.2:+50836999 None:intergenic
TAAAGGCTACTGTCGCGAAA+AGG 0.486640 2.2:-50835165 MS.gene57478:CDS
AGTTATGTACTCGAAATTGT+AGG 0.496998 2.2:+50835843 None:intergenic
ACCATTGGCTGCATCTGCTA+TGG 0.500352 2.2:+50836743 None:intergenic
TGTATGTTTCATGGCCACTT+TGG 0.501309 2.2:+50835701 None:intergenic
AAAAGGTCGTTGCAAGGGAA+TGG 0.502322 2.2:-50835148 MS.gene57478:CDS
GTATACCACGTGACATCATA+TGG 0.507968 2.2:-50836813 MS.gene57478:CDS
GGTGCACAAATTAATCTTGA+AGG 0.512636 2.2:-50836678 MS.gene57478:CDS
GATTCAAACTTTAGAGGAGG+AGG 0.515787 2.2:-50835802 MS.gene57478:CDS
TCCTTCTTCTCAACCAGCTG+AGG 0.516756 2.2:-50837012 MS.gene57478:intron
CATTGTTACAATAAGGCCAC+TGG 0.522380 2.2:-50835502 MS.gene57478:CDS
ATGGAAGGCATCAGTAACCT+TGG 0.524813 2.2:-50836702 MS.gene57478:CDS
ACTTTGGACTAAAATTCCAT+TGG 0.525845 2.2:+50835717 None:intergenic
GTCGCGAAAAGGTCGTTGCA+AGG 0.526188 2.2:-50835154 MS.gene57478:CDS
TCATATGGTGCAGACCCAAC+AGG 0.528783 2.2:-50836798 MS.gene57478:CDS
TGTTGATAGGAACGTTGCGA+CGG 0.532688 2.2:-50835197 MS.gene57478:CDS
AGGTAGTTTCAAATAAATTC+CGG 0.535034 2.2:+50835468 None:intergenic
AAACACGAAAGGAATTTCTC+AGG 0.538491 2.2:-50835634 MS.gene57478:CDS
AGTTACCGGTAACCAGCGTT+GGG 0.539499 2.2:-50836626 MS.gene57478:CDS
AAGTTACCGGTAACCAGCGT+TGG 0.543200 2.2:-50836627 MS.gene57478:CDS
GATTAATTTGTGCACCTCCA+AGG 0.547641 2.2:+50836685 None:intergenic
ATGTTCTCTGGATCAAATCA+AGG 0.548900 2.2:-50835280 MS.gene57478:CDS
TCGAAATTGTAGGATGATGA+AGG 0.552977 2.2:+50835853 None:intergenic
CGAAAGGAATTTCTCAGGCA+CGG 0.554053 2.2:-50835629 MS.gene57478:CDS
AAGGATTGTGAATTCATACA+TGG 0.557886 2.2:-50835431 MS.gene57478:CDS
AGATGATGGTGTTGAAGCAA+TGG 0.559821 2.2:+50837049 None:intergenic
GGAATTTATTTGAAACTACC+TGG 0.560862 2.2:-50835466 MS.gene57478:CDS
GAAGGTGCAAAAGAAGATGA+TGG 0.564254 2.2:+50837035 None:intergenic
TGACAGCGTTATCGTTACCT+TGG 0.565717 2.2:+50835596 None:intergenic
GCCATAGCAGATGCAGCCAA+TGG 0.568367 2.2:-50836744 MS.gene57478:CDS
GAAAGGAATTTCTCAGGCAC+GGG 0.570441 2.2:-50835628 MS.gene57478:CDS
GCACAAATTAATCTTGAAGG+TGG 0.580445 2.2:-50836675 MS.gene57478:CDS
TCGCGAAAAGGTCGTTGCAA+GGG 0.583554 2.2:-50835153 MS.gene57478:CDS
TTGGATTCAAACTTTAGAGG+AGG 0.586410 2.2:-50835805 MS.gene57478:CDS
TACCATGATGTTCCCAACGC+TGG 0.586939 2.2:+50836614 None:intergenic
AGCGAAGGATATTTGATGGA+AGG 0.588639 2.2:-50836717 MS.gene57478:CDS
CTTGGCCAACACATAACCGC+CGG 0.589091 2.2:-50835664 MS.gene57478:CDS
GAAGGCATCAGTAACCTTGG+AGG 0.592934 2.2:-50836699 MS.gene57478:CDS
ATCCTTGTTTGTGTTAAACC+AGG 0.593807 2.2:+50835448 None:intergenic
CGGCGGTTATGTGTTGGCCA+AGG 0.595647 2.2:+50835665 None:intergenic
TCATGCTATCAGAAATGTGT+CGG 0.600119 2.2:-50835026 MS.gene57478:CDS
TCCGGTACCGCCGAATCCAG+TGG 0.602767 2.2:+50835486 None:intergenic
GCTGGTTGAGAAGAAGGAGA+AGG 0.608402 2.2:+50837017 None:intergenic
TTGATGGAAATACAGATACA+TGG 0.610495 2.2:-50835967 MS.gene57478:intron
GCAGCCAATGGTCCAAGCGA+AGG 0.613400 2.2:-50836732 MS.gene57478:CDS
GGCACGGGAATAAACATCCA+AGG 0.627419 2.2:-50835613 MS.gene57478:CDS
TTTCGAGTACATAACTCTCA+AGG 0.629286 2.2:-50835836 MS.gene57478:CDS
AATAAGGCCACTGGATTCGG+CGG 0.629975 2.2:-50835493 MS.gene57478:CDS
TACCTGGTTTAACACAAACA+AGG 0.631189 2.2:-50835450 MS.gene57478:CDS
AACCAGCGTTGGGAACATCA+TGG 0.637299 2.2:-50836616 MS.gene57478:intron
TTGATAGGAACGTTGCGACG+GGG 0.641777 2.2:-50835195 MS.gene57478:CDS
TGCAGAGAAAATTACAACAT+CGG 0.644559 2.2:+50835573 None:intergenic
ATCATTAGGAAAATCATCTG+AGG 0.645215 2.2:+50835930 None:intergenic
GGCCAACACATAACCGCCGG+AGG 0.651132 2.2:-50835661 MS.gene57478:CDS
TTCGTGTTTATCGCCTCCGG+CGG 0.651957 2.2:+50835648 None:intergenic
CTGCACCATATGATGTCACG+TGG 0.660523 2.2:+50836808 None:intergenic
CCGGAGGCGATAAACACGAA+AGG 0.669288 2.2:-50835645 MS.gene57478:CDS
ATTGTGTTGAAATCTATGAA+AGG 0.670877 2.2:-50835334 MS.gene57478:CDS
ACACAATATTGGCATCACCA+AGG 0.694010 2.2:+50835350 None:intergenic
ATGGAAATACAGATACATGG+AGG 0.764204 2.2:-50835964 MS.gene57478:intron

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! AAAACTAATTATTTGACAAT+TGG - chr2.2:50835613-50835632 MS.gene57478:CDS 15.0%
!! TTTCATACTGTAAAAAAAAA+AGG + chr2.2:50835574-50835593 None:intergenic 15.0%
!!! GACTATTTCTATTAATATAT+TGG - chr2.2:50835757-50835776 MS.gene57478:CDS 15.0%
!! ATGTACTAAACAAAAATCAA+AGG + chr2.2:50835084-50835103 None:intergenic 20.0%
!!! AATTTTTGATTTCTTTGGAA+TGG + chr2.2:50836020-50836039 None:intergenic 20.0%
!!! AGTACTGAACATTTTTAATA+AGG + chr2.2:50836964-50836983 None:intergenic 20.0%
!!! ATAATTAGTTTTTCTACAGT+TGG + chr2.2:50835605-50835624 None:intergenic 20.0%
!!! ATAGTCAATATTCATTTTCT+TGG + chr2.2:50835743-50835762 None:intergenic 20.0%
!!! GTCAAAATTTTTGATTTCTT+TGG + chr2.2:50836025-50836044 None:intergenic 20.0%
!!! TGAACATTTTTAATAAGGTT+AGG + chr2.2:50836959-50836978 None:intergenic 20.0%
!!! TTAGTATATTTTAGTCATGA+TGG - chr2.2:50835695-50835714 MS.gene57478:CDS 20.0%
!!! TTTGGAATGGAAAAAAAAAA+GGG + chr2.2:50836007-50836026 None:intergenic 20.0%
! AAGTTTGTCATATCTATTGT+CGG - chr2.2:50835150-50835169 MS.gene57478:CDS 25.0%
! ACAATAGATATGACAAACTT+AGG + chr2.2:50835150-50835169 None:intergenic 25.0%
! AGGTAGTTTCAAATAAATTC+CGG + chr2.2:50836576-50836595 None:intergenic 25.0%
! AGTTTCTTATGTATGTTTCA+TGG + chr2.2:50836352-50836371 None:intergenic 25.0%
! ATTGTGTTGAAATCTATGAA+AGG - chr2.2:50836707-50836726 MS.gene57478:CDS 25.0%
! CATAGATTTCAACACAATAT+TGG + chr2.2:50836705-50836724 None:intergenic 25.0%
! CTTGTCTCAAAAATTGAATT+TGG + chr2.2:50835665-50835684 None:intergenic 25.0%
! TTATTGGATTCAAACTTTAG+AGG - chr2.2:50836233-50836252 MS.gene57478:intron 25.0%
! TTGATCAAGTTATTGTTGAT+AGG - chr2.2:50836831-50836850 MS.gene57478:CDS 25.0%
! TTGTTTATTCAATAAAGCGA+GGG - chr2.2:50835798-50835817 MS.gene57478:CDS 25.0%
! TTTGTTTATTCAATAAAGCG+AGG - chr2.2:50835797-50835816 MS.gene57478:CDS 25.0%
!!! CTTTGGAATGGAAAAAAAAA+AGG + chr2.2:50836008-50836027 None:intergenic 25.0%
!!! GATGATTTTTTTGTTGTGTA+GGG - chr2.2:50835189-50835208 MS.gene57478:CDS 25.0%
!!! TCTATGAAAGGTGTTTTAAA+TGG - chr2.2:50836719-50836738 MS.gene57478:CDS 25.0%
!!! TGATGATTTTTTTGTTGTGT+AGG - chr2.2:50835188-50835207 MS.gene57478:CDS 25.0%
!!! TTTTTGTTGTTGTGATTTGA+TGG - chr2.2:50836058-50836077 MS.gene57478:intron 25.0%
!!! TTTTTTTAGTAGAGAACACT+AGG - chr2.2:50835103-50835122 MS.gene57478:CDS 25.0%
AAACAAAAATCAAAGGCTCA+AGG + chr2.2:50835077-50835096 None:intergenic 30.0%
AAGGATTGTGAATTCATACA+TGG - chr2.2:50836610-50836629 MS.gene57478:intron 30.0%
ACATTTCTGATAGCATGATT+AGG + chr2.2:50837013-50837032 None:intergenic 30.0%
ACCTCATCAAATGAAAATGA+TGG - chr2.2:50836155-50836174 MS.gene57478:intron 30.0%
ACTTGATCAACTTGATGAAA+TGG + chr2.2:50836821-50836840 None:intergenic 30.0%
ACTTTGGACTAAAATTCCAT+TGG + chr2.2:50836327-50836346 None:intergenic 30.0%
AGTTATGTACTCGAAATTGT+AGG + chr2.2:50836201-50836220 None:intergenic 30.0%
ATCATTAGGAAAATCATCTG+AGG + chr2.2:50836114-50836133 None:intergenic 30.0%
ATTACACATTTCACAACCAA+TGG - chr2.2:50836308-50836327 MS.gene57478:intron 30.0%
CTAGAAATATGAAGTTGAAC+AGG + chr2.2:50836662-50836681 None:intergenic 30.0%
TACATAAGAAACTCTTTCCT+TGG - chr2.2:50836359-50836378 MS.gene57478:intron 30.0%
TGCAGAGAAAATTACAACAT+CGG + chr2.2:50836471-50836490 None:intergenic 30.0%
TGGTGTACATTGTTACAATA+AGG - chr2.2:50836532-50836551 MS.gene57478:intron 30.0%
TGTTTATTCAATAAAGCGAG+GGG - chr2.2:50835799-50835818 MS.gene57478:CDS 30.0%
TTGATGGAAATACAGATACA+TGG - chr2.2:50836074-50836093 MS.gene57478:intron 30.0%
! AGCAAATGAAACAATTTTGC+AGG + chr2.2:50835455-50835474 None:intergenic 30.0%
! GGAATTTATTTGAAACTACC+TGG - chr2.2:50836575-50836594 MS.gene57478:intron 30.0%
! TCAGATGATTTTCCTAATGA+TGG - chr2.2:50836113-50836132 MS.gene57478:intron 30.0%
! TCCATCATTTTCATTTGATG+AGG + chr2.2:50836159-50836178 None:intergenic 30.0%
!! AAAATCATCTGAGGCTTTTA+TGG + chr2.2:50836105-50836124 None:intergenic 30.0%
!! AATGGAATTTTAGTCCAAAG+TGG - chr2.2:50836326-50836345 MS.gene57478:intron 30.0%
!! AGGTGTTGAAATTGTTCATT+TGG - chr2.2:50836781-50836800 MS.gene57478:CDS 30.0%
!! ATTGTTGATAACATGTTCTC+TGG - chr2.2:50836749-50836768 MS.gene57478:CDS 30.0%
!! ATTTCTAGTAGTTTCTTCCT+TGG - chr2.2:50836674-50836693 MS.gene57478:CDS 30.0%
!!! GGAAGTGTTTTAAGACTTTA+TGG - chr2.2:50835634-50835653 MS.gene57478:CDS 30.0%
!!! TGTTTTAAGACTTTATGGCT+AGG - chr2.2:50835639-50835658 MS.gene57478:CDS 30.0%
AAACACGAAAGGAATTTCTC+AGG - chr2.2:50836407-50836426 MS.gene57478:intron 35.0%
AACTCTCAAGGATCTCTTAT+TGG - chr2.2:50836217-50836236 MS.gene57478:intron 35.0%
ACTGTAGAAACTAATTCTCG+AGG - chr2.2:50835518-50835537 MS.gene57478:CDS 35.0%
ATGGAAATACAGATACATGG+AGG - chr2.2:50836077-50836096 MS.gene57478:intron 35.0%
ATGTTCTCTGGATCAAATCA+AGG - chr2.2:50836761-50836780 MS.gene57478:CDS 35.0%
CAGTACTCATTAAGTTCAAC+AGG - chr2.2:50836977-50836996 MS.gene57478:intron 35.0%
GCACAAATTAATCTTGAAGG+TGG - chr2.2:50835366-50835385 MS.gene57478:CDS 35.0%
GGTGCACAAATTAATCTTGA+AGG - chr2.2:50835363-50835382 MS.gene57478:CDS 35.0%
TACCTGGTTTAACACAAACA+AGG - chr2.2:50836591-50836610 MS.gene57478:intron 35.0%
TCAATGATGTAGCCATCATT+AGG + chr2.2:50836128-50836147 None:intergenic 35.0%
TCATGCTATCAGAAATGTGT+CGG - chr2.2:50837015-50837034 MS.gene57478:CDS 35.0%
TCGAAATTGTAGGATGATGA+AGG + chr2.2:50836191-50836210 None:intergenic 35.0%
TGATTGAATTATACCTCAGC+TGG + chr2.2:50835045-50835064 None:intergenic 35.0%
! ATCCTTGTTTGTGTTAAACC+AGG + chr2.2:50836596-50836615 None:intergenic 35.0%
! GGAATCATGTCTTGTTAAAG+AGG + chr2.2:50834974-50834993 None:intergenic 35.0%
! GTAATTTTCTCTGCAGCTAT+AGG - chr2.2:50836476-50836495 MS.gene57478:intron 35.0%
! TTGGATTCAAACTTTAGAGG+AGG - chr2.2:50836236-50836255 MS.gene57478:intron 35.0%
! TTTCGAGTACATAACTCTCA+AGG - chr2.2:50836205-50836224 MS.gene57478:intron 35.0%
AACAGCCTCTTGTGTAAAAC+AGG - chr2.2:50835887-50835906 MS.gene57478:CDS 40.0%
ACACAATATTGGCATCACCA+AGG + chr2.2:50836694-50836713 None:intergenic 40.0%
ACAGCCTCTTGTGTAAAACA+GGG - chr2.2:50835888-50835907 MS.gene57478:CDS 40.0%
AGCGAAGGATATTTGATGGA+AGG - chr2.2:50835324-50835343 MS.gene57478:CDS 40.0%
CAGAAAGTCACTCAAGTTAC+CGG - chr2.2:50835401-50835420 MS.gene57478:CDS 40.0%
CATTGTTACAATAAGGCCAC+TGG - chr2.2:50836539-50836558 MS.gene57478:intron 40.0%
CGTACAATACACCAAATGGT+GGG - chr2.2:50835919-50835938 MS.gene57478:CDS 40.0%
CTCTTGTGTAAAACAGGGTA+AGG - chr2.2:50835893-50835912 MS.gene57478:CDS 40.0%
GCTATAGGAATCATGGTAAC+TGG - chr2.2:50836491-50836510 MS.gene57478:intron 40.0%
GGAAAAAAAAAAGGGCAACC+CGG + chr2.2:50835999-50836018 None:intergenic 40.0%
GTATACCACGTGACATCATA+TGG - chr2.2:50835228-50835247 MS.gene57478:CDS 40.0%
GTTGCGTACAATACACCAAA+TGG - chr2.2:50835915-50835934 MS.gene57478:CDS 40.0%
GTTGTTGTCATGTGACTGAA+AGG - chr2.2:50835844-50835863 MS.gene57478:CDS 40.0%
TACAATAAGGCCACTGGATT+CGG - chr2.2:50836545-50836564 MS.gene57478:intron 40.0%
TCCAAGCGAAGGATATTTGA+TGG - chr2.2:50835320-50835339 MS.gene57478:CDS 40.0%
TCCATCAAATATCCTTCGCT+TGG + chr2.2:50835324-50835343 None:intergenic 40.0%
TGCTATCTGAATTGCCTGTT+GGG + chr2.2:50835260-50835279 None:intergenic 40.0%
TGTATGTTTCATGGCCACTT+TGG + chr2.2:50836343-50836362 None:intergenic 40.0%
! AGATGATGGTGTTGAAGCAA+TGG + chr2.2:50834995-50835014 None:intergenic 40.0%
! CTTACCCTGTTTTACACAAG+AGG + chr2.2:50835895-50835914 None:intergenic 40.0%
! GAAGGTGCAAAAGAAGATGA+TGG + chr2.2:50835009-50835028 None:intergenic 40.0%
! GATTCAAACTTTAGAGGAGG+AGG - chr2.2:50836239-50836258 MS.gene57478:intron 40.0%
! GGTCAAGCAAACACATTTTC+TGG - chr2.2:50836512-50836531 MS.gene57478:intron 40.0%
!! GATTAATTTGTGCACCTCCA+AGG + chr2.2:50835359-50835378 None:intergenic 40.0%
AAAAGGTCGTTGCAAGGGAA+TGG - chr2.2:50836893-50836912 MS.gene57478:intron 45.0%
AATAAAGCGAGGGGTAACCT+TGG - chr2.2:50835808-50835827 MS.gene57478:CDS 45.0%
ATATCCTTCGCTTGGACCAT+TGG + chr2.2:50835316-50835335 None:intergenic 45.0%
CGAAAGGAATTTCTCAGGCA+CGG - chr2.2:50836412-50836431 MS.gene57478:intron 45.0%
CTCTGCAGCTATAGGAATCA+TGG - chr2.2:50836484-50836503 MS.gene57478:intron 45.0%
GAAAGGAATTTCTCAGGCAC+GGG - chr2.2:50836413-50836432 MS.gene57478:intron 45.0%
GATGTCACGTGGTATACTCT+TGG + chr2.2:50835225-50835244 None:intergenic 45.0%
GCGTACAATACACCAAATGG+TGG - chr2.2:50835918-50835937 MS.gene57478:CDS 45.0%
GTGCTATCTGAATTGCCTGT+TGG + chr2.2:50835261-50835280 None:intergenic 45.0%
TAAAGGCTACTGTCGCGAAA+AGG - chr2.2:50836876-50836895 MS.gene57478:intron 45.0%
TCATGTGACTGAAAGGTCAC+TGG - chr2.2:50835851-50835870 MS.gene57478:CDS 45.0%
TGACAGCGTTATCGTTACCT+TGG + chr2.2:50836448-50836467 None:intergenic 45.0%
TGCAAGGGAATGGAACTTCA+TGG - chr2.2:50836903-50836922 MS.gene57478:intron 45.0%
TGCGACGGGGATGAATTTAA+AGG - chr2.2:50836859-50836878 MS.gene57478:intron 45.0%
! AGTCATGATGGTGAAGCAGT+TGG - chr2.2:50835707-50835726 MS.gene57478:CDS 45.0%
! TGTTGATAGGAACGTTGCGA+CGG - chr2.2:50836844-50836863 MS.gene57478:intron 45.0%
! TTACACAAGAGGCTGTTTCC+AGG + chr2.2:50835884-50835903 None:intergenic 45.0%
!! ATGGAAGGCATCAGTAACCT+TGG - chr2.2:50835339-50835358 MS.gene57478:CDS 45.0%
AAGTTACCGGTAACCAGCGT+TGG - chr2.2:50835414-50835433 MS.gene57478:CDS 50.0%
AATAAGGCCACTGGATTCGG+CGG - chr2.2:50836548-50836567 MS.gene57478:intron 50.0%
ACCTCAGCTGGTTGAGAAGA+AGG + chr2.2:50835033-50835052 None:intergenic 50.0%
AGTTACCGGTAACCAGCGTT+GGG - chr2.2:50835415-50835434 MS.gene57478:CDS 50.0%
CAACTTTACCAGTTGCGCCA+AGG + chr2.2:50835828-50835847 None:intergenic 50.0%
CCTTTCGTGTTTATCGCCTC+CGG + chr2.2:50836399-50836418 None:intergenic 50.0%
CTGCACCATATGATGTCACG+TGG + chr2.2:50835236-50835255 None:intergenic 50.0%
GCTGGTTGAGAAGAAGGAGA+AGG + chr2.2:50835027-50835046 None:intergenic 50.0%
GGCACGGGAATAAACATCCA+AGG - chr2.2:50836428-50836447 MS.gene57478:intron 50.0%
TACCATGATGTTCCCAACGC+TGG + chr2.2:50835430-50835449 None:intergenic 50.0%
TCATATGGTGCAGACCCAAC+AGG - chr2.2:50835243-50835262 MS.gene57478:CDS 50.0%
TCCTTCTTCTCAACCAGCTG+AGG - chr2.2:50835029-50835048 MS.gene57478:CDS 50.0%
TCGCGAAAAGGTCGTTGCAA+GGG - chr2.2:50836888-50836907 MS.gene57478:intron 50.0%
TTGATAGGAACGTTGCGACG+GGG - chr2.2:50836846-50836865 MS.gene57478:intron 50.0%
! AACCAGCGTTGGGAACATCA+TGG - chr2.2:50835425-50835444 MS.gene57478:CDS 50.0%
! ACCATTGGCTGCATCTGCTA+TGG + chr2.2:50835301-50835320 None:intergenic 50.0%
! ATGTTCCCAACGCTGGTTAC+CGG + chr2.2:50835423-50835442 None:intergenic 50.0%
! GTTGATAGGAACGTTGCGAC+GGG - chr2.2:50836845-50836864 MS.gene57478:intron 50.0%
!! AAGGTCACTGGTTCAAGTCC+TGG - chr2.2:50835863-50835882 MS.gene57478:CDS 50.0%
!! CGCTGCGAATGTTTTTGTCG+CGG - chr2.2:50837072-50837091 MS.gene57478:CDS 50.0%
!! GAAGGCATCAGTAACCTTGG+AGG - chr2.2:50835342-50835361 MS.gene57478:CDS 50.0%
!! TGCGAATGTTTTTGTCGCGG+TGG - chr2.2:50837075-50837094 MS.gene57478:CDS 50.0%
ACTAGAGCTCCCGCATATGC+AGG + chr2.2:50835973-50835992 None:intergenic 55.0%
CCGGAGGCGATAAACACGAA+AGG - chr2.2:50836396-50836415 MS.gene57478:intron 55.0%
CTAGAGCTCCCGCATATGCA+GGG + chr2.2:50835972-50835991 None:intergenic 55.0%
CTTGGCCAACACATAACCGC+CGG - chr2.2:50836377-50836396 MS.gene57478:intron 55.0%
GCCATAGCAGATGCAGCCAA+TGG - chr2.2:50835297-50835316 MS.gene57478:CDS 55.0%
GTCGCGAAAAGGTCGTTGCA+AGG - chr2.2:50836887-50836906 MS.gene57478:intron 55.0%
TTCGTGTTTATCGCCTCCGG+CGG + chr2.2:50836396-50836415 None:intergenic 55.0%
AGGGGTAACCTTGGCGCAAC+TGG - chr2.2:50835817-50835836 MS.gene57478:CDS 60.0%
ATATGCAGGGTCCGGGATGC+AGG + chr2.2:50835959-50835978 None:intergenic 60.0%
ATCCCGGACCCTGCATATGC+GGG - chr2.2:50835961-50835980 MS.gene57478:intron 60.0%
CATCCCGGACCCTGCATATG+CGG - chr2.2:50835960-50835979 MS.gene57478:intron 60.0%
GCAGCCAATGGTCCAAGCGA+AGG - chr2.2:50835309-50835328 MS.gene57478:CDS 60.0%
TATGCAGGGTCCGGGATGCA+GGG + chr2.2:50835958-50835977 None:intergenic 60.0%
! ATGCGGGAGCTCTAGTGCAC+CGG - chr2.2:50835977-50835996 MS.gene57478:intron 60.0%
! CAAATGGTGGGACCCCTTCC+CGG - chr2.2:50835931-50835950 MS.gene57478:CDS 60.0%
! CGGCGGTTATGTGTTGGCCA+AGG + chr2.2:50836379-50836398 None:intergenic 60.0%
CGCCTCCGGCGGTTATGTGT+TGG + chr2.2:50836385-50836404 None:intergenic 65.0%
CGGGAAGGGGTCCCACCATT+TGG + chr2.2:50835933-50835952 None:intergenic 65.0%
CTCCCGCATATGCAGGGTCC+GGG + chr2.2:50835966-50835985 None:intergenic 65.0%
GCCACTGGATTCGGCGGTAC+CGG - chr2.2:50836554-50836573 MS.gene57478:intron 65.0%
GCTCCCGCATATGCAGGGTC+CGG + chr2.2:50835967-50835986 None:intergenic 65.0%
GGCCAACACATAACCGCCGG+AGG - chr2.2:50836380-50836399 MS.gene57478:intron 65.0%
TCCGGTACCGCCGAATCCAG+TGG + chr2.2:50836558-50836577 None:intergenic 65.0%
! TGCGGGAGCTCTAGTGCACC+GGG - chr2.2:50835978-50835997 MS.gene57478:intron 65.0%
AGGGTCCGGGATGCAGGGTC+CGG + chr2.2:50835953-50835972 None:intergenic 70.0%
CCTTCCCGGACCCTGCATCC+CGG - chr2.2:50835945-50835964 MS.gene57478:CDS 70.0%
CGGGATGCAGGGTCCGGGAA+GGG + chr2.2:50835947-50835966 None:intergenic 70.0%
GGGATGCAGGGTCCGGGAAG+GGG + chr2.2:50835946-50835965 None:intergenic 70.0%
CCGGGATGCAGGGTCCGGGA+AGG + chr2.2:50835948-50835967 None:intergenic 75.0%
GGGTCCGGGATGCAGGGTCC+GGG + chr2.2:50835952-50835971 None:intergenic 75.0%


Chromosome Type Strat End Strand Name
chr2.2 gene 50834946 50837117 50834946 ID=MS.gene57478
chr2.2 mRNA 50834946 50837117 50834946 ID=MS.gene57478.t1;Parent=MS.gene57478
chr2.2 exon 50837013 50837117 50837013 ID=MS.gene57478.t1.exon1;Parent=MS.gene57478.t1
chr2.2 CDS 50837013 50837117 50837013 ID=cds.MS.gene57478.t1;Parent=MS.gene57478.t1
chr2.2 exon 50836617 50836853 50836617 ID=MS.gene57478.t1.exon2;Parent=MS.gene57478.t1
chr2.2 CDS 50836617 50836853 50836617 ID=cds.MS.gene57478.t1;Parent=MS.gene57478.t1
chr2.2 exon 50834946 50835974 50834946 ID=MS.gene57478.t1.exon3;Parent=MS.gene57478.t1
chr2.2 CDS 50834946 50835974 50834946 ID=cds.MS.gene57478.t1;Parent=MS.gene57478.t1
Gene Sequence

>MS.gene57478

ATGAGAAGATTGCAATCATTTAAGACCTCTTTAACAAGACATGATTCCATTGCTTCAACACCATCATCTTCTTTTGCACCTTCTCCTTCTTCTCAACCAGCTGAGGTATAATTCAATCATATACACAACCTTGAGCCTTTGATTTTTGTTTAGTACATTTTTTTAGTAGAGAACACTAGGAACAATGTCTATAACATTGTACCTAAGTTTGTCATATCTATTGTCGGAAAATATCGAATATTTGATGATTTTTTTGTTGTGTAGGGTATGAACAATCCAAGAGTATACCACGTGACATCATATGGTGCAGACCCAACAGGCAATTCAGATAGCACTGAAGCATTACTTGCAGCCATAGCAGATGCAGCCAATGGTCCAAGCGAAGGATATTTGATGGAAGGCATCAGTAACCTTGGAGGTGCACAAATTAATCTTGAAGGTGGAAATTACATGATCAGAAAGTCACTCAAGTTACCGGTAACCAGCGTTGGGAACATCATGGTACGCCTGCAAAATTGTTTCATTTGCTAATTACATAAAAAAAAACTATAAATCAAATATCTCTGCGTACAACTGTAGAAACTAATTCTCGAGGATGCACAACTGTAGAAAAATTTCATGCTGTCCTTTTTTTTTTACAGTATGAAAAGTATCTACCAACTGTAGAAAAACTAATTATTTGACAATTGGAAGTGTTTTAAGACTTTATGGCTAGGCCAAATTCAATTTTTGAGACAAGTAGTCTCAATTTAGTATATTTTAGTCATGATGGTGAAGCAGTTGGTATAACTAGACCAAGAAAATGAATATTGACTATTTCTATTAATATATTGGAAAAGTATTTTTTTTCATTTGTTTATTCAATAAAGCGAGGGGTAACCTTGGCGCAACTGGTAAAGTTGTTGTCATGTGACTGAAAGGTCACTGGTTCAAGTCCTGGAAACAGCCTCTTGTGTAAAACAGGGTAAGGTTGCGTACAATACACCAAATGGTGGGACCCCTTCCCGGACCCTGCATCCCGGACCCTGCATATGCGGGAGCTCTAGTGCACCGGGTTGCCCTTTTTTTTTTCCATTCCAAAGAAATCAAAAATTTTGACATGTTGATTTGATTTTTTGTTGTTGTGATTTGATGGAAATACAGATACATGGAGGAACCATAAAAGCCTCAGATGATTTTCCTAATGATGGCTACATCATTGATTTATCAACCTCATCAAATGAAAATGATGGAAAAAATTCACCTTCATCATCCTACAATTTCGAGTACATAACTCTCAAGGATCTCTTATTGGATTCAAACTTTAGAGGAGGAGGCATTTCAGTCATAAACTCACTCAGAACAAACATAGACAACTGTTACATTACACATTTCACAACCAATGGAATTTTAGTCCAAAGTGGCCATGAAACATACATAAGAAACTCTTTCCTTGGCCAACACATAACCGCCGGAGGCGATAAACACGAAAGGAATTTCTCAGGCACGGGAATAAACATCCAAGGTAACGATAACGCTGTCACCGATGTTGTAATTTTCTCTGCAGCTATAGGAATCATGGTAACTGGTCAAGCAAACACATTTTCTGGTGTACATTGTTACAATAAGGCCACTGGATTCGGCGGTACCGGAATTTATTTGAAACTACCTGGTTTAACACAAACAAGGATTGTGAATTCATACATGGATTATACAAGTATTGTTGCTGAAGATCCTGTTCAACTTCATATTTCTAGTAGTTTCTTCCTTGGTGATGCCAATATTGTGTTGAAATCTATGAAAGGTGTTTTAAATGGTGTTACTATTGTTGATAACATGTTCTCTGGATCAAATCAAGGTGTTGAAATTGTTCATTTGGATAAATCAAATAGTCCATTTCATCAAGTTGATCAAGTTATTGTTGATAGGAACGTTGCGACGGGGATGAATTTAAAGGCTACTGTCGCGAAAAGGTCGTTGCAAGGGAATGGAACTTCATGGAATGTTGATTTTAACAACATTCTTCTTTTTCCTAACCTTATTAAAAATGTTCAGTACTCATTAAGTTCAACAGGTAGCAGTTTTCCTAATCATGCTATCAGAAATGTGTCGGACAATCGCGTTGTGATTGAAACGAATGAAGCAGTCGCTGCGAATGTTTTTGTCGCGGTGGATCAAAGTATGTCAAGTTGA
Protein sequence

>MS.gene57478.t1

MRRLQSFKTSLTRHDSIASTPSSSFAPSPSSQPAEGMNNPRVYHVTSYGADPTGNSDSTEALLAAIADAANGPSEGYLMEGISNLGGAQINLEGGNYMIRKSLKLPVTSVGNIMIHGGTIKASDDFPNDGYIIDLSTSSNENDGKNSPSSSYNFEYITLKDLLLDSNFRGGGISVINSLRTNIDNCYITHFTTNGILVQSGHETYIRNSFLGQHITAGGDKHERNFSGTGINIQGNDNAVTDVVIFSAAIGIMVTGQANTFSGVHCYNKATGFGGTGIYLKLPGLTQTRIVNSYMDYTSIVAEDPVQLHISSSFFLGDANIVLKSMKGVLNGVTIVDNMFSGSNQGVEIVHLDKSNSPFHQVDQVIVDRNVATGMNLKATVAKRSLQGNGTSWNVDFNNILLFPNLIKNVQYSLSSTGSSFPNHAIRNVSDNRVVIETNEAVAANVFVAVDQSMSS