Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene57491.t1 | RHN40044.1 | 67.9 | 296 | 69 | 10 | 1 | 290 | 1 | 276 | 3.70E-100 | 374.8 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene57491.t1 | Q7M443 | 35.0 | 274 | 151 | 9 | 14 | 280 | 2 | 255 | 6.3e-32 | 139.4 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene57491.t1 | A0A396GKD7 | 67.9 | 296 | 69 | 10 | 1 | 290 | 1 | 276 | 2.7e-100 | 374.8 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene049681 | MS.gene57491 | 0.804801 | 1.81E-49 | -1.69E-46 |
| MS.gene051860 | MS.gene57491 | 0.830668 | 2.58E-55 | -1.69E-46 |
| MS.gene054183 | MS.gene57491 | 0.813081 | 3.07E-51 | -1.69E-46 |
| MS.gene059294 | MS.gene57491 | 0.815537 | 8.79E-52 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene57491.t1 | MTR_0002s0040 | 53.698 | 311 | 103 | 10 | 1 | 290 | 1 | 291 | 2.50e-95 | 283 |
| MS.gene57491.t1 | MTR_0263s0020 | 56.164 | 292 | 96 | 9 | 1 | 280 | 1 | 272 | 4.21e-94 | 280 |
| MS.gene57491.t1 | MTR_1457s0010 | 66.234 | 231 | 54 | 8 | 65 | 291 | 1 | 211 | 2.88e-93 | 275 |
| MS.gene57491.t1 | MTR_0002s1060 | 51.546 | 291 | 108 | 11 | 4 | 280 | 6 | 277 | 3.70e-75 | 232 |
| MS.gene57491.t1 | MTR_0062s0020 | 38.542 | 288 | 140 | 8 | 14 | 280 | 4 | 275 | 1.39e-48 | 164 |
| MS.gene57491.t1 | MTR_0062s0090 | 39.510 | 286 | 138 | 10 | 14 | 280 | 4 | 273 | 8.98e-48 | 161 |
| MS.gene57491.t1 | MTR_0062s0040 | 39.510 | 286 | 138 | 10 | 14 | 280 | 4 | 273 | 8.98e-48 | 161 |
| MS.gene57491.t1 | MTR_0062s0060 | 39.510 | 286 | 138 | 10 | 14 | 280 | 4 | 273 | 8.98e-48 | 161 |
| MS.gene57491.t1 | MTR_0443s0040 | 39.175 | 291 | 132 | 12 | 14 | 280 | 16 | 285 | 1.26e-47 | 161 |
| MS.gene57491.t1 | MTR_0062s0170 | 36.304 | 303 | 151 | 10 | 1 | 280 | 1 | 284 | 8.30e-39 | 139 |
| MS.gene57491.t1 | MTR_0062s0110 | 36.304 | 303 | 151 | 10 | 1 | 280 | 1 | 284 | 8.30e-39 | 139 |
| MS.gene57491.t1 | MTR_0062s0130 | 36.304 | 303 | 151 | 10 | 1 | 280 | 1 | 284 | 8.30e-39 | 139 |
| MS.gene57491.t1 | MTR_2g036240 | 36.304 | 303 | 151 | 10 | 1 | 280 | 1 | 284 | 8.30e-39 | 139 |
| MS.gene57491.t1 | MTR_2g036260 | 36.304 | 303 | 151 | 10 | 1 | 280 | 1 | 284 | 8.30e-39 | 139 |
| MS.gene57491.t1 | MTR_2g036290 | 36.304 | 303 | 151 | 10 | 1 | 280 | 1 | 284 | 8.30e-39 | 139 |
| MS.gene57491.t1 | MTR_8g046730 | 36.304 | 303 | 151 | 10 | 1 | 280 | 1 | 284 | 8.30e-39 | 139 |
| MS.gene57491.t1 | MTR_6g079630 | 34.182 | 275 | 156 | 8 | 12 | 280 | 32 | 287 | 3.22e-37 | 134 |
| MS.gene57491.t1 | MTR_2g096030 | 32.990 | 291 | 163 | 10 | 14 | 291 | 31 | 302 | 1.39e-33 | 124 |
| MS.gene57491.t1 | MTR_2g096040 | 33.202 | 253 | 145 | 6 | 39 | 283 | 26 | 262 | 2.08e-28 | 110 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Find 0 sgRNAs with CRISPR-Local
Find 80 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !!! | AATATAAAAGATTTTGGGTA+TGG | - | 57513:11480-11499 | MS.gene57491:intergenic | 20.0% |
| !! | CAGGGAATATAAAAGATTTT+GGG | - | 57513:11485-11504 | MS.gene57491:intergenic | 25.0% |
| !! | TCAGGGAATATAAAAGATTT+TGG | - | 57513:11486-11505 | MS.gene57491:intergenic | 25.0% |
| !! | TTCATTTTACGAGAATACAT+TGG | + | 57513:11447-11466 | MS.gene57491:CDS | 25.0% |
| AGAGAAAGGATATTTAGTAG+GGG | - | 57513:11739-11758 | MS.gene57491:intergenic | 30.0% | |
| ATTTCGTTGTAGTCAATATC+AGG | - | 57513:11504-11523 | MS.gene57491:intergenic | 30.0% | |
| GAGAGAAAGGATATTTAGTA+GGG | - | 57513:11740-11759 | MS.gene57491:intergenic | 30.0% | |
| TATAAAAAGACTCAAGGAAG+AGG | + | 57513:11902-11921 | MS.gene57491:CDS | 30.0% | |
| TTTCGTTGTAGTCAATATCA+GGG | - | 57513:11503-11522 | MS.gene57491:intergenic | 30.0% | |
| ! | GTAGTGTTCATTAGTTTCAT+GGG | - | 57513:11958-11977 | MS.gene57491:intergenic | 30.0% |
| ! | TGTAGTGTTCATTAGTTTCA+TGG | - | 57513:11959-11978 | MS.gene57491:intergenic | 30.0% |
| !! | AATCGATTTCCATGTCATTT+TGG | + | 57513:11521-11540 | MS.gene57491:CDS | 30.0% |
| !! | ACTTTTGAAAAACTCCTAGT+AGG | + | 57513:12107-12126 | MS.gene57491:CDS | 30.0% |
| !!! | TCTTTTTCAATAGGAGAGAA+AGG | - | 57513:11753-11772 | MS.gene57491:intergenic | 30.0% |
| AAGATTTCTCCAACTACGTT+GGG | + | 57513:11874-11893 | MS.gene57491:CDS | 35.0% | |
| ACCACAGTGACGATATTAAT+TGG | + | 57513:11994-12013 | MS.gene57491:CDS | 35.0% | |
| ACTTACAACGAAGATGGAAA+AGG | + | 57513:11558-11577 | MS.gene57491:CDS | 35.0% | |
| ATCTTCGTTGTAAGTCTCAT+GGG | - | 57513:11553-11572 | MS.gene57491:intergenic | 35.0% | |
| CCACAGTGACGATATTAATT+GGG | + | 57513:11995-12014 | MS.gene57491:CDS | 35.0% | |
| CTCCTATTGAAAAAGAATCC+TGG | + | 57513:11757-11776 | MS.gene57491:CDS | 35.0% | |
| GAGAAAGGATATTTAGTAGG+GGG | - | 57513:11738-11757 | MS.gene57491:intergenic | 35.0% | |
| GGACGTTATAAAAAGACTCA+AGG | + | 57513:11896-11915 | MS.gene57491:CDS | 35.0% | |
| GGAGAGAAAGGATATTTAGT+AGG | - | 57513:11741-11760 | MS.gene57491:intergenic | 35.0% | |
| GTTGGAGAAATCTTCTTCAT+TGG | - | 57513:11868-11887 | MS.gene57491:intergenic | 35.0% | |
| ! | TGTTCATTAGTTTCATGGGA+TGG | - | 57513:11954-11973 | MS.gene57491:intergenic | 35.0% |
| !!! | CACCAGGATTCTTTTTCAAT+AGG | - | 57513:11762-11781 | MS.gene57491:intergenic | 35.0% |
| AAAAGACTCAAGGAAGAGGT+AGG | + | 57513:11906-11925 | MS.gene57491:CDS | 40.0% | |
| AACACTACAAGCTACTCTAC+AGG | + | 57513:11970-11989 | MS.gene57491:CDS | 40.0% | |
| ACACTACAAGCTACTCTACA+GGG | + | 57513:11971-11990 | MS.gene57491:CDS | 40.0% | |
| AGATTTCTCCAACTACGTTG+GGG | + | 57513:11875-11894 | MS.gene57491:CDS | 40.0% | |
| ATCCAAGACTACACGTTTGA+TGG | + | 57513:11813-11832 | MS.gene57491:CDS | 40.0% | |
| CAGATATCAAGATCCCAATC+GGG | - | 57513:11600-11619 | MS.gene57491:intergenic | 40.0% | |
| CATCTTCGTTGTAAGTCTCA+TGG | - | 57513:11554-11573 | MS.gene57491:intergenic | 40.0% | |
| CATGAGACTTACAACGAAGA+TGG | + | 57513:11552-11571 | MS.gene57491:CDS | 40.0% | |
| CCCAATTAATATCGTCACTG+TGG | - | 57513:11998-12017 | MS.gene57491:intergenic | 40.0% | |
| CGGAAAATTCTATCCCGATT+GGG | + | 57513:11584-11603 | MS.gene57491:CDS | 40.0% | |
| GAAGATTTCTCCAACTACGT+TGG | + | 57513:11873-11892 | MS.gene57491:CDS | 40.0% | |
| GAATCGATCAACAGTGTTAC+CGG | - | 57513:11430-11449 | MS.gene57491:intergenic | 40.0% | |
| GATCTATGTTAGACTCGCAA+GGG | + | 57513:12076-12095 | MS.gene57491:CDS | 40.0% | |
| GATTGGGATCTTGATATCTG+CGG | + | 57513:11600-11619 | MS.gene57491:CDS | 40.0% | |
| GCAGATATCAAGATCCCAAT+CGG | - | 57513:11601-11620 | MS.gene57491:intergenic | 40.0% | |
| TATCAAGCTCAAGTATCCCA+AGG | + | 57513:11641-11660 | MS.gene57491:CDS | 40.0% | |
| TCGTGATATACAAAGTGTGC+AGG | + | 57513:11689-11708 | MS.gene57491:CDS | 40.0% | |
| TGATCTATGTTAGACTCGCA+AGG | + | 57513:12075-12094 | MS.gene57491:CDS | 40.0% | |
| TTGCGAGTCTAACATAGATC+AGG | - | 57513:12076-12095 | MS.gene57491:intergenic | 40.0% | |
| ! | AACGCAGTGGCATTTTCATT+TGG | + | 57513:12216-12235 | MS.gene57491:CDS | 40.0% |
| ! | CCAATCGGGATAGAATTTTC+CGG | - | 57513:11586-11605 | MS.gene57491:intergenic | 40.0% |
| !! | GATCTTTTTGAGCGAATCGA+CGG | - | 57513:11793-11812 | MS.gene57491:intergenic | 40.0% |
| ACAAAGTCAGTCTCGTCAGT+GGG | - | 57513:12050-12069 | MS.gene57491:intergenic | 45.0% | |
| ACGAAGACATCTAGTGGAAG+TGG | - | 57513:12167-12186 | MS.gene57491:intergenic | 45.0% | |
| AGTTTCATGGGATGGAGCAA+TGG | - | 57513:11946-11965 | MS.gene57491:intergenic | 45.0% | |
| CCGGAAAATTCTATCCCGAT+TGG | + | 57513:11583-11602 | MS.gene57491:CDS | 45.0% | |
| CTAAGTACCACCTTGACCTT+GGG | - | 57513:11660-11679 | MS.gene57491:intergenic | 45.0% | |
| GCAGGATATCATCAAGCACA+AGG | + | 57513:11707-11726 | MS.gene57491:CDS | 45.0% | |
| GCGTTGAACCTTCATGAGAT+CGG | - | 57513:12201-12220 | MS.gene57491:intergenic | 45.0% | |
| GGAAGAGGTAGGTATCAATG+TGG | + | 57513:11917-11936 | MS.gene57491:CDS | 45.0% | |
| GGTTAGGCTGCATGTAGAAT+TGG | - | 57513:12028-12047 | MS.gene57491:intergenic | 45.0% | |
| GTCAAGGTGGTACTTAGCAT+TGG | + | 57513:11663-11682 | MS.gene57491:CDS | 45.0% | |
| GTTGTAAGTCTCATGGGCAA+AGG | - | 57513:11547-11566 | MS.gene57491:intergenic | 45.0% | |
| TACAAAGTCAGTCTCGTCAG+TGG | - | 57513:12051-12070 | MS.gene57491:intergenic | 45.0% | |
| TATAACGTCCCCAACGTAGT+TGG | - | 57513:11886-11905 | MS.gene57491:intergenic | 45.0% | |
| TGCCATCAAACGTGTAGTCT+TGG | - | 57513:11818-11837 | MS.gene57491:intergenic | 45.0% | |
| ! | AACGAAGATGGAAAAGGCAC+CGG | + | 57513:11564-11583 | MS.gene57491:CDS | 45.0% |
| ! | AAGGTGGTACTTAGCATTGG+TGG | + | 57513:11666-11685 | MS.gene57491:CDS | 45.0% |
| AGCTTGATATCCTGCACACG+TGG | - | 57513:11630-11649 | MS.gene57491:intergenic | 50.0% | |
| ATCGACGGCATTATCACACC+AGG | - | 57513:11778-11797 | MS.gene57491:intergenic | 50.0% | |
| ATTACGTCCGTCACGTGGAT+CGG | - | 57513:12141-12160 | MS.gene57491:intergenic | 50.0% | |
| CAAAGTCAGTCTCGTCAGTG+GGG | - | 57513:12049-12068 | MS.gene57491:intergenic | 50.0% | |
| CAAGTATCCCAAGGTCAAGG+TGG | + | 57513:11650-11669 | MS.gene57491:CDS | 50.0% | |
| CTCATGAAGGTTCAACGCAG+TGG | + | 57513:12203-12222 | MS.gene57491:CDS | 50.0% | |
| CTCGCAACGAAGACATCTAG+TGG | - | 57513:12173-12192 | MS.gene57491:intergenic | 50.0% | |
| GAGTTGCTCCGATCTCATGA+AGG | + | 57513:12190-12209 | MS.gene57491:CDS | 50.0% | |
| GCTAAGTACCACCTTGACCT+TGG | - | 57513:11661-11680 | MS.gene57491:intergenic | 50.0% | |
| GCTCAAGTATCCCAAGGTCA+AGG | + | 57513:11647-11666 | MS.gene57491:CDS | 50.0% | |
| GGGCAAAGGCCAAAATGACA+TGG | - | 57513:11533-11552 | MS.gene57491:intergenic | 50.0% | |
| GGAACATTACGTCCGTCACG+TGG | - | 57513:12146-12165 | MS.gene57491:intergenic | 55.0% | |
| TCAGTCTCGTCAGTGGGGTT+AGG | - | 57513:12044-12063 | MS.gene57491:intergenic | 55.0% | |
| ! | GATCGGTGCTTGCTCCTACT+AGG | - | 57513:12124-12143 | MS.gene57491:intergenic | 55.0% |
| CTGCGGTATACCACGTGTGC+AGG | + | 57513:11617-11636 | MS.gene57491:CDS | 60.0% | |
| !! | GCAAGCACCGATCCACGTGA+CGG | + | 57513:12131-12150 | MS.gene57491:CDS | 60.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| 57513 | gene | 11411 | 12290 | 11411 | ID=MS.gene57491 |
| 57513 | mRNA | 11411 | 12290 | 11411 | ID=MS.gene57491.t1;Parent=MS.gene57491 |
| 57513 | exon | 11411 | 12290 | 11411 | ID=MS.gene57491.t1.exon1;Parent=MS.gene57491.t1 |
| 57513 | CDS | 11411 | 12290 | 11411 | ID=cds.MS.gene57491.t1;Parent=MS.gene57491.t1 |
| Gene Sequence |
| Protein sequence |
