Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene57603.t1 | XP_024637947.1 | 96.8 | 93 | 3 | 0 | 1 | 93 | 330 | 422 | 1.50E-42 | 181.8 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene57603.t1 | A0A396IIY0 | 96.8 | 93 | 3 | 0 | 1 | 93 | 330 | 422 | 1.1e-42 | 181.8 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene051856 | MS.gene57603 | 0.862648 | 4.37E-64 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Find 24 sgRNAs with CRISPR-Local
Find 25 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| TAATATCTATCTTGAATTAG+AGG | 0.304710 | 8.4:-9042956 | MS.gene57603:CDS |
| TGTTATGTCAGAGAAGCTTT+TGG | 0.350236 | 8.4:-9043022 | MS.gene57603:CDS |
| GATAAGTAAGCCAACAGATT+GGG | 0.381656 | 8.4:-9042929 | MS.gene57603:CDS |
| TTCTGTTGTTGATGAAGAAA+TGG | 0.389880 | 8.4:-9042992 | MS.gene57603:CDS |
| TGGATCTGCTAGTGGATTGG+TGG | 0.433958 | 8.4:-9042905 | MS.gene57603:CDS |
| TAGCTCTTTACATGACTTGA+GGG | 0.437021 | 8.4:-9043094 | MS.gene57603:CDS |
| AAATATGAACACTGGTAGAA+TGG | 0.455673 | 8.4:-9043154 | None:intergenic |
| TGATAAGTAAGCCAACAGAT+TGG | 0.468059 | 8.4:-9042930 | MS.gene57603:CDS |
| TCTTCCATGGGAGTAGAAAC+AGG | 0.475802 | 8.4:+9043126 | None:intergenic |
| GGGTGGATCTGCTAGTGGAT+TGG | 0.502731 | 8.4:-9042908 | MS.gene57603:CDS |
| TTACATGACTTGAGGGCTTG+TGG | 0.510182 | 8.4:-9043087 | MS.gene57603:CDS |
| GTTTCTACTCCCATGGAAGA+TGG | 0.512936 | 8.4:-9043123 | MS.gene57603:CDS |
| GTAGCTCTTTACATGACTTG+AGG | 0.518635 | 8.4:-9043095 | MS.gene57603:CDS |
| GCTACACAGTCCATCTTCCA+TGG | 0.535967 | 8.4:+9043113 | None:intergenic |
| GGATCTGCTAGTGGATTGGT+GGG | 0.540365 | 8.4:-9042904 | MS.gene57603:CDS |
| ACCACCTGTTTCTACTCCCA+TGG | 0.552610 | 8.4:-9043130 | MS.gene57603:CDS |
| ATAAGTAAGCCAACAGATTG+GGG | 0.574399 | 8.4:-9042928 | MS.gene57603:CDS |
| TTTCCTGAAAATATGAACAC+TGG | 0.585214 | 8.4:-9043162 | None:intergenic |
| GCAGATCCACCCCAATCTGT+TGG | 0.588399 | 8.4:+9042919 | None:intergenic |
| CTACACAGTCCATCTTCCAT+GGG | 0.610941 | 8.4:+9043114 | None:intergenic |
| GATTGGGGTGGATCTGCTAG+TGG | 0.632406 | 8.4:-9042913 | MS.gene57603:CDS |
| CATGACTTGAGGGCTTGTGG+TGG | 0.643012 | 8.4:-9043084 | MS.gene57603:CDS |
| AGTAAGCCAACAGATTGGGG+TGG | 0.660771 | 8.4:-9042925 | MS.gene57603:CDS |
| TCCATGGGAGTAGAAACAGG+TGG | 0.762840 | 8.4:+9043129 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | TAATATCTATCTTGAATTAG+AGG | - | chr8.4:9043085-9043104 | MS.gene57603:CDS | 20.0% |
| ! | TTCTGTTGTTGATGAAGAAA+TGG | - | chr8.4:9043049-9043068 | MS.gene57603:CDS | 30.0% |
| ATAAGTAAGCCAACAGATTG+GGG | - | chr8.4:9043113-9043132 | MS.gene57603:CDS | 35.0% | |
| GATAAGTAAGCCAACAGATT+GGG | - | chr8.4:9043112-9043131 | MS.gene57603:CDS | 35.0% | |
| TAGCTCTTTACATGACTTGA+GGG | - | chr8.4:9042947-9042966 | MS.gene57603:CDS | 35.0% | |
| TGATAAGTAAGCCAACAGAT+TGG | - | chr8.4:9043111-9043130 | MS.gene57603:CDS | 35.0% | |
| ! | GTTATGTCAGAGAAGCTTTT+GGG | - | chr8.4:9043020-9043039 | MS.gene57603:CDS | 35.0% |
| ! | TGTTATGTCAGAGAAGCTTT+TGG | - | chr8.4:9043019-9043038 | MS.gene57603:CDS | 35.0% |
| ! | TTATGTCAGAGAAGCTTTTG+GGG | - | chr8.4:9043021-9043040 | MS.gene57603:CDS | 35.0% |
| GTAGCTCTTTACATGACTTG+AGG | - | chr8.4:9042946-9042965 | MS.gene57603:CDS | 40.0% | |
| ! | TATGTCAGAGAAGCTTTTGG+GGG | - | chr8.4:9043022-9043041 | MS.gene57603:CDS | 40.0% |
| CTACACAGTCCATCTTCCAT+GGG | + | chr8.4:9042930-9042949 | None:intergenic | 45.0% | |
| GTTTCTACTCCCATGGAAGA+TGG | - | chr8.4:9042918-9042937 | MS.gene57603:CDS | 45.0% | |
| TCTTCCATGGGAGTAGAAAC+AGG | + | chr8.4:9042918-9042937 | None:intergenic | 45.0% | |
| TTACATGACTTGAGGGCTTG+TGG | - | chr8.4:9042954-9042973 | MS.gene57603:CDS | 45.0% | |
| AGTAAGCCAACAGATTGGGG+TGG | - | chr8.4:9043116-9043135 | MS.gene57603:CDS | 50.0% | |
| GCTACACAGTCCATCTTCCA+TGG | + | chr8.4:9042931-9042950 | None:intergenic | 50.0% | |
| TCCATGGGAGTAGAAACAGG+TGG | + | chr8.4:9042915-9042934 | None:intergenic | 50.0% | |
| !! | ACCACCTGTTTCTACTCCCA+TGG | - | chr8.4:9042911-9042930 | MS.gene57603:CDS | 50.0% |
| !! | GGATCTGCTAGTGGATTGGT+GGG | - | chr8.4:9043137-9043156 | MS.gene57603:CDS | 50.0% |
| !! | TGGATCTGCTAGTGGATTGG+TGG | - | chr8.4:9043136-9043155 | MS.gene57603:CDS | 50.0% |
| CATGACTTGAGGGCTTGTGG+TGG | - | chr8.4:9042957-9042976 | MS.gene57603:CDS | 55.0% | |
| GATTGGGGTGGATCTGCTAG+TGG | - | chr8.4:9043128-9043147 | MS.gene57603:CDS | 55.0% | |
| GCAGATCCACCCCAATCTGT+TGG | + | chr8.4:9043125-9043144 | None:intergenic | 55.0% | |
| ! | GGGTGGATCTGCTAGTGGAT+TGG | - | chr8.4:9043133-9043152 | MS.gene57603:CDS | 55.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr8.4 | gene | 9042891 | 9043172 | 9042891 | ID=MS.gene57603 |
| chr8.4 | mRNA | 9042891 | 9043172 | 9042891 | ID=MS.gene57603.t1;Parent=MS.gene57603 |
| chr8.4 | exon | 9042891 | 9043172 | 9042891 | ID=MS.gene57603.t1.exon1;Parent=MS.gene57603.t1 |
| chr8.4 | CDS | 9042891 | 9043172 | 9042891 | ID=cds.MS.gene57603.t1;Parent=MS.gene57603.t1 |
| Gene Sequence |
| Protein sequence |
