Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene57605.t1 | XP_024637947.1 | 96.8 | 93 | 3 | 0 | 1 | 93 | 330 | 422 | 1.50E-42 | 181.8 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene57605.t1 | A0A396IIY0 | 96.8 | 93 | 3 | 0 | 1 | 93 | 330 | 422 | 1.1e-42 | 181.8 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene051856 | MS.gene57605 | 0.868259 | 7.46E-66 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Find 24 sgRNAs with CRISPR-Local
Find 25 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| TAATATCTATCTTGAATTAG+AGG | 0.304710 | 8.4:-9058708 | MS.gene57605:CDS |
| TGTTATGTCAGAGAAGCTTT+TGG | 0.350236 | 8.4:-9058774 | MS.gene57605:CDS |
| GATAAGTAAGCCAACAGATT+GGG | 0.381656 | 8.4:-9058681 | MS.gene57605:CDS |
| TTCTGTTGTTGATGAAGAAA+TGG | 0.389880 | 8.4:-9058744 | MS.gene57605:CDS |
| TGGATCTGCTAGTGGATTGG+TGG | 0.433958 | 8.4:-9058657 | MS.gene57605:CDS |
| TAGCTCTTTACATGACTTGA+GGG | 0.437021 | 8.4:-9058846 | MS.gene57605:CDS |
| AAATATGAACACTGGTAGAA+TGG | 0.455673 | 8.4:-9058906 | None:intergenic |
| TGATAAGTAAGCCAACAGAT+TGG | 0.468059 | 8.4:-9058682 | MS.gene57605:CDS |
| TCTTCCATGGGAGTAGAAAC+AGG | 0.475802 | 8.4:+9058878 | None:intergenic |
| GGGTGGATCTGCTAGTGGAT+TGG | 0.502731 | 8.4:-9058660 | MS.gene57605:CDS |
| TTACATGACTTGAGGGCTTG+TGG | 0.510182 | 8.4:-9058839 | MS.gene57605:CDS |
| GTTTCTACTCCCATGGAAGA+TGG | 0.512936 | 8.4:-9058875 | MS.gene57605:CDS |
| GTAGCTCTTTACATGACTTG+AGG | 0.518635 | 8.4:-9058847 | MS.gene57605:CDS |
| GCTACACAGTCCATCTTCCA+TGG | 0.535967 | 8.4:+9058865 | None:intergenic |
| GGATCTGCTAGTGGATTGGT+GGG | 0.540365 | 8.4:-9058656 | MS.gene57605:CDS |
| ACCACCTGTTTCTACTCCCA+TGG | 0.552610 | 8.4:-9058882 | MS.gene57605:CDS |
| ATAAGTAAGCCAACAGATTG+GGG | 0.574399 | 8.4:-9058680 | MS.gene57605:CDS |
| TTTCCTGAAAATATGAACAC+TGG | 0.585214 | 8.4:-9058914 | None:intergenic |
| GCAGATCCACCCCAATCTGT+TGG | 0.588399 | 8.4:+9058671 | None:intergenic |
| CTACACAGTCCATCTTCCAT+GGG | 0.610941 | 8.4:+9058866 | None:intergenic |
| GATTGGGGTGGATCTGCTAG+TGG | 0.632406 | 8.4:-9058665 | MS.gene57605:CDS |
| CATGACTTGAGGGCTTGTGG+TGG | 0.643012 | 8.4:-9058836 | MS.gene57605:CDS |
| AGTAAGCCAACAGATTGGGG+TGG | 0.660771 | 8.4:-9058677 | MS.gene57605:CDS |
| TCCATGGGAGTAGAAACAGG+TGG | 0.762840 | 8.4:+9058881 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | TAATATCTATCTTGAATTAG+AGG | - | chr8.4:9058837-9058856 | MS.gene57605:CDS | 20.0% |
| ! | TTCTGTTGTTGATGAAGAAA+TGG | - | chr8.4:9058801-9058820 | MS.gene57605:CDS | 30.0% |
| ATAAGTAAGCCAACAGATTG+GGG | - | chr8.4:9058865-9058884 | MS.gene57605:CDS | 35.0% | |
| GATAAGTAAGCCAACAGATT+GGG | - | chr8.4:9058864-9058883 | MS.gene57605:CDS | 35.0% | |
| TAGCTCTTTACATGACTTGA+GGG | - | chr8.4:9058699-9058718 | MS.gene57605:CDS | 35.0% | |
| TGATAAGTAAGCCAACAGAT+TGG | - | chr8.4:9058863-9058882 | MS.gene57605:CDS | 35.0% | |
| ! | GTTATGTCAGAGAAGCTTTT+GGG | - | chr8.4:9058772-9058791 | MS.gene57605:CDS | 35.0% |
| ! | TGTTATGTCAGAGAAGCTTT+TGG | - | chr8.4:9058771-9058790 | MS.gene57605:CDS | 35.0% |
| ! | TTATGTCAGAGAAGCTTTTG+GGG | - | chr8.4:9058773-9058792 | MS.gene57605:CDS | 35.0% |
| GTAGCTCTTTACATGACTTG+AGG | - | chr8.4:9058698-9058717 | MS.gene57605:CDS | 40.0% | |
| ! | TATGTCAGAGAAGCTTTTGG+GGG | - | chr8.4:9058774-9058793 | MS.gene57605:CDS | 40.0% |
| CTACACAGTCCATCTTCCAT+GGG | + | chr8.4:9058682-9058701 | None:intergenic | 45.0% | |
| GTTTCTACTCCCATGGAAGA+TGG | - | chr8.4:9058670-9058689 | MS.gene57605:CDS | 45.0% | |
| TCTTCCATGGGAGTAGAAAC+AGG | + | chr8.4:9058670-9058689 | None:intergenic | 45.0% | |
| TTACATGACTTGAGGGCTTG+TGG | - | chr8.4:9058706-9058725 | MS.gene57605:CDS | 45.0% | |
| AGTAAGCCAACAGATTGGGG+TGG | - | chr8.4:9058868-9058887 | MS.gene57605:CDS | 50.0% | |
| GCTACACAGTCCATCTTCCA+TGG | + | chr8.4:9058683-9058702 | None:intergenic | 50.0% | |
| TCCATGGGAGTAGAAACAGG+TGG | + | chr8.4:9058667-9058686 | None:intergenic | 50.0% | |
| !! | ACCACCTGTTTCTACTCCCA+TGG | - | chr8.4:9058663-9058682 | MS.gene57605:CDS | 50.0% |
| !! | GGATCTGCTAGTGGATTGGT+GGG | - | chr8.4:9058889-9058908 | MS.gene57605:CDS | 50.0% |
| !! | TGGATCTGCTAGTGGATTGG+TGG | - | chr8.4:9058888-9058907 | MS.gene57605:CDS | 50.0% |
| CATGACTTGAGGGCTTGTGG+TGG | - | chr8.4:9058709-9058728 | MS.gene57605:CDS | 55.0% | |
| GATTGGGGTGGATCTGCTAG+TGG | - | chr8.4:9058880-9058899 | MS.gene57605:CDS | 55.0% | |
| GCAGATCCACCCCAATCTGT+TGG | + | chr8.4:9058877-9058896 | None:intergenic | 55.0% | |
| ! | GGGTGGATCTGCTAGTGGAT+TGG | - | chr8.4:9058885-9058904 | MS.gene57605:CDS | 55.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr8.4 | gene | 9058643 | 9058924 | 9058643 | ID=MS.gene57605 |
| chr8.4 | mRNA | 9058643 | 9058924 | 9058643 | ID=MS.gene57605.t1;Parent=MS.gene57605 |
| chr8.4 | exon | 9058643 | 9058924 | 9058643 | ID=MS.gene57605.t1.exon1;Parent=MS.gene57605.t1 |
| chr8.4 | CDS | 9058643 | 9058924 | 9058643 | ID=cds.MS.gene57605.t1;Parent=MS.gene57605.t1 |
| Gene Sequence |
| Protein sequence |
